Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,937,523 A→G L239P (CTG→CCG)  yieO ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,5230AG100.0% 779.7 / NA 244L239P (CTG→CCG) yieOputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (120/124);  total (120/124)

Only 100 of 245 total aligned reads displayed.

ACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCAGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGA  >  NC_000913/3937433‑3937612
                                                                                          |                                                                                         
aCGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGt                                                                                         >  1:30898/1‑93 (MQ=255)
  gATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTaa                                                                                       >  2:2675183/1‑93 (MQ=255)
   aTACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAAc                                                                                      <  1:1484875/93‑1 (MQ=255)
     aCCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAAccc                                                                                    <  2:4637707/93‑1 (MQ=255)
     aCCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAAccc                                                                                    >  1:2576475/1‑93 (MQ=255)
      ccGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCg                                                                                   >  1:4005589/1‑93 (MQ=255)
       cGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGa                                                                                  <  1:2214131/93‑1 (MQ=255)
       cGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGa                                                                                  <  1:246778/93‑1 (MQ=255)
        gATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAt                                                                                 <  2:3105293/93‑1 (MQ=255)
        gATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAt                                                                                 >  2:4162731/1‑93 (MQ=255)
        gATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAt                                                                                 >  2:3902104/1‑93 (MQ=255)
         aTCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATg                                                                                >  2:4108858/1‑93 (MQ=255)
          tCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGc                                                                               <  1:1349933/93‑1 (MQ=255)
               aaaGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTg                                                                          >  1:4266313/1‑93 (MQ=255)
                  gTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACa                                                                       >  2:4486356/1‑93 (MQ=255)
                   tGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACaa                                                                      <  2:3830876/93‑1 (MQ=255)
                   tGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACaa                                                                      >  2:4275835/1‑93 (MQ=255)
                    gCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAAt                                                                     >  1:543712/1‑93 (MQ=255)
                    gCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAAt                                                                     <  1:3795558/93‑1 (MQ=255)
                     cGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAAtt                                                                    <  1:500745/93‑1 (MQ=255)
                        gTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTACATGGAGAATATAGAGAGGCGGTAACCCGATGCTGGTGACAATTccc                                                                 <  2:824086/93‑1 (MQ=255)
                          tttAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGt                                                               <  2:4203773/93‑1 (MQ=255)
                           ttAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTc                                                              <  1:3279447/93‑1 (MQ=255)
                            taaataaaTCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCa                                                             >  2:2975035/1‑93 (MQ=255)
                             aaataaatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCaa                                                            <  1:4342822/93‑1 (MQ=255)
                             aaataaatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCaa                                                            <  1:1268698/93‑1 (MQ=255)
                               ataaatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAgg                                                          >  1:84234/1‑93 (MQ=255)
                                taaatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGc                                                         >  2:1665680/1‑93 (MQ=255)
                                 aaatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCa                                                        >  2:2635938/1‑93 (MQ=255)
                                  aatCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCaa                                                       <  1:4444037/93‑1 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATc                                                     >  2:3053310/1‑93 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATc                                                     <  2:1764700/93‑1 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATc                                                     <  1:529990/93‑1 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATc                                                     <  1:1824949/93‑1 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATc                                                     >  1:1728408/1‑93 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGAAATc                                                     >  1:1596929/1‑93 (MQ=255)
                                    tCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTAACGTCAAGGCAATc                                                     >  1:2778637/1‑93 (MQ=255)
                                     cTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATcc                                                    >  1:4476138/1‑93 (MQ=255)
                                      tAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCa                                                   <  1:2674297/93‑1 (MQ=255)
                                      tAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCa                                                   <  2:319459/93‑1 (MQ=255)
                                       aaTGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAg                                                  >  2:3776711/1‑93 (MQ=255)
                                        aTGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGc                                                 >  1:1236588/1‑93 (MQ=255)
                                         tGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCt                                                >  1:3775509/1‑93 (MQ=255)
                                         tGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCt                                                <  1:1309282/93‑1 (MQ=255)
                                         tGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCt                                                >  2:4427073/1‑93 (MQ=255)
                                            aaTTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGc                                             <  1:340853/93‑1 (MQ=255)
                                            aaTTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGc                                             >  1:3998456/1‑93 (MQ=255)
                                            aaTTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGc                                             >  1:2262567/1‑93 (MQ=255)
                                             aTTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCg                                            >  1:2817857/1‑93 (MQ=255)
                                             aTTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCg                                            >  1:681822/1‑93 (MQ=255)
                                               tAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGAc                                          <  2:1736662/93‑1 (MQ=255)
                                                aaTGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACa                                         >  1:2872256/1‑93 (MQ=255)
                                                  tGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAAt                                       >  2:283177/1‑93 (MQ=255)
                                                   gggTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATc                                      <  1:2892373/93‑1 (MQ=255)
                                                     gTTTGGCGTGCGTCTTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCtt                                    <  2:1084428/93‑1 (MQ=255)
                                                     gTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCtt                                    >  2:1200201/1‑93 (MQ=255)
                                                      tttGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCttt                                   >  1:745454/1‑93 (MQ=255)
                                                      tttGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCttt                                   >  1:1544653/1‑93 (MQ=255)
                                                        tGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTc                                 <  2:2586767/93‑1 (MQ=255)
                                                         ggCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTcc                                <  1:4378829/93‑1 (MQ=255)
                                                          gcgtgcgtCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTccc                               <  1:3581703/93‑1 (MQ=255)
                                                          gcgtgcgtCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTccc                               <  1:4103250/93‑1 (MQ=255)
                                                            gtgcgtCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCg                             <  1:4640001/93‑1 (MQ=255)
                                                              gcgtCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGaa                           <  2:1878208/93‑1 (MQ=255)
                                                              gcgtCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGaa                           <  2:2563486/93‑1 (MQ=255)
                                                               cgtcgtGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAAt                          <  2:836516/93‑1 (MQ=255)
                                                                gtcgtGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATa                         >  1:3477679/1‑93 (MQ=255)
                                                                gtcgtGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATa                         >  2:2035457/1‑93 (MQ=255)
                                                                 tcgtGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAg                        >  2:2773123/1‑93 (MQ=255)
                                                                 tcgtGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAg                        <  1:2097123/93‑1 (MQ=255)
                                                                   gtGCATGGAGAATATAGAGAAGCGGTAATCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGCCGACACTATTTTTCCCGAATAgtc                      >  2:3230569/1‑91 (MQ=255)
                                                                    tGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGctc                     <  1:2613324/93‑1 (MQ=255)
                                                                    tGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGctc                     <  2:941185/93‑1 (MQ=255)
                                                                     gCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGctct                    <  1:995044/93‑1 (MQ=255)
                                                                      cATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTa                   <  2:2304839/93‑1 (MQ=255)
                                                                      cATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTa                   <  1:594148/93‑1 (MQ=255)
                                                                      cATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTa                   <  2:2231815/93‑1 (MQ=255)
                                                                       aTGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAt                  <  2:4586791/93‑1 (MQ=255)
                                                                       aTGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAt                  <  2:1433110/93‑1 (MQ=255)
                                                                       aTGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAt                  <  1:1424887/93‑1 (MQ=255)
                                                                       aTGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAt                  <  2:4053061/93‑1 (MQ=255)
                                                                        tGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAtt                 >  1:2167300/1‑93 (MQ=255)
                                                                        tGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAtt                 >  2:723617/1‑93 (MQ=255)
                                                                        tGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAtt                 >  1:3643999/1‑93 (MQ=255)
                                                                        tGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAtt                 >  2:894238/1‑93 (MQ=255)
                                                                         ggAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTc                <  2:1334271/93‑1 (MQ=255)
                                                                         ggAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTGCCCCGAATAGCTCTATTc                <  2:4348765/93‑1 (MQ=255)
                                                                          gagaATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTcc               <  2:3450373/93‑1 (MQ=255)
                                                                             aaTATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCt            >  2:4533556/1‑93 (MQ=255)
                                                                               tataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTg          >  2:3710463/1‑93 (MQ=255)
                                                                               tataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTg          >  2:1495811/1‑93 (MQ=255)
                                                                               tataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTg          <  2:599879/93‑1 (MQ=255)
                                                                               tataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTg          <  1:1074740/93‑1 (MQ=255)
                                                                                ataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTga         <  1:4137856/93‑1 (MQ=255)
                                                                                ataGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTga         >  2:2178801/1‑93 (MQ=255)
                                                                                  agagAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTgaga       <  2:2338170/93‑1 (MQ=255)
                                                                                  agagAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTgaga       >  1:3234717/1‑93 (MQ=255)
                                                                                   gagaAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTgagaa      <  1:1175663/93‑1 (MQ=255)
                                                                                     gaAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTgagaaga    >  1:792193/1‑93 (MQ=255)
                                                                                     gaAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTgagaaga    <  1:208084/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCAGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGA  >  NC_000913/3937433‑3937612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCAGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACAA  >  NC_000913/3937430‑3937616
                                                                                             |                                                                                             
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGCATATAGAGAAGCG                                                                                               >  SRR4302535.4140045/1‑94 (MQ=60)
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCG                                                                                               >  SRR4302535.949035/1‑94 (MQ=60)
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCG                                                                                               <  SRR4302535.275524/94‑1 (MQ=60)
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCG                                                                                               >  SRR4302535.3607333/1‑94 (MQ=60)
 CTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGG                                                                                              >  SRR4302535.859711/1‑94 (MQ=60)
 CTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGG                                                                                              >  SRR4302535.3172744/1‑94 (MQ=60)
  TACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGT                                                                                             >  SRR4302535.31137/1‑94 (MQ=60)
    CGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAA                                                                                           <  SRR4302535.774331/94‑1 (MQ=60)
     GATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAAC                                                                                          <  SRR4302535.4215280/94‑1 (MQ=60)
      ATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACC                                                                                         <  SRR4302535.1495501/94‑1 (MQ=60)
       TACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCC                                                                                        <  SRR4302535.3307325/94‑1 (MQ=60)
       TACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCC                                                                                        >  SRR4302535.2594993/1‑94 (MQ=60)
       TACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCC                                                                                        >  SRR4302535.399035/1‑94 (MQ=60)
        ACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCG                                                                                       >  SRR4302535.4032989/1‑94 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     >  SRR4302535.3273002/1‑94 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     <  SRR4302535.248469/94‑1 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     <  SRR4302535.696883/94‑1 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     <  SRR4302535.3489282/94‑1 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     <  SRR4302535.2229898/94‑1 (MQ=60)
          CGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGAT                                                                                     <  SRR4302535.2925390/94‑1 (MQ=60)
            ATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGC                                                                                   >  SRR4302535.4153225/1‑94 (MQ=60)
             TCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCT                                                                                  <  SRR4302535.1359574/94‑1 (MQ=60)
              CGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTG                                                                                 <  SRR4302535.2217049/94‑1 (MQ=60)
              CGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTG                                                                                 <  SRR4302535.412337/94‑1 (MQ=60)
              CGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTG                                                                                 >  SRR4302535.1560082/1‑94 (MQ=60)
               GAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGG                                                                                <  SRR4302535.2153982/94‑1 (MQ=60)
                AGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGT                                                                               >  SRR4302535.891465/1‑94 (MQ=60)
                 GAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTG                                                                              >  SRR4302535.4295525/1‑94 (MQ=60)
                   AAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGAC                                                                            >  SRR4302535.3045320/1‑94 (MQ=60)
                    AGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACA                                                                           >  SRR4302535.1306000/1‑94 (MQ=60)
                    AGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACA                                                                           >  SRR4302535.3938885/1‑94 (MQ=60)
                    AGTGAGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACA                                                                           >  SRR4302535.506554/1‑94 (MQ=60)
                     GTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAA                                                                          >  SRR4302535.740153/1‑94 (MQ=60)
                      TGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAAT                                                                         >  SRR4302535.547514/1‑94 (MQ=60)
                       GCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATT                                                                        <  SRR4302535.3821722/94‑1 (MQ=60)
                       GCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATT                                                                        >  SRR4302535.3704699/1‑94 (MQ=60)
                        CGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTA                                                                       <  SRR4302535.504251/94‑1 (MQ=60)
                         GGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTAC                                                                      <  SRR4302535.2638955/94‑1 (MQ=60)
                         GGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTAC                                                                      <  SRR4302535.225317/94‑1 (MQ=60)
                           GTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCG                                                                    <  SRR4302535.4051100/94‑1 (MQ=60)
                              TTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCA                                                                 <  SRR4302535.3302224/94‑1 (MQ=60)
                               TAAATAAATCTAATGAAATTAATGGGTTTAGCGTGCGTCGGGCATGGAGAAGATACAGAAGCGGAAACGCAATGCTGGTGACAATTACCGTCAA                                                                >  SRR4302535.1323343/1‑94 (MQ=60)
                                AAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAG                                                               <  SRR4302535.2097015/94‑1 (MQ=60)
                                AAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAG                                                               <  SRR4302535.4386875/94‑1 (MQ=60)
                                AAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAG                                                               <  SRR4302535.4372520/94‑1 (MQ=60)
                                AAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAG                                                               <  SRR4302535.1277769/94‑1 (MQ=60)
                                 AATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGG                                                              <  SRR4302535.2145013/94‑1 (MQ=60)
                                 AATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGG                                                              >  SRR4302535.84827/1‑94 (MQ=60)
                                  ATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGC                                                             >  SRR4302535.2870974/1‑94 (MQ=60)
                                  ATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGC                                                             >  SRR4302535.1262330/1‑94 (MQ=60)
                                  ATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGC                                                             >  SRR4302535.804659/1‑94 (MQ=60)
                                  ATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGC                                                             >  SRR4302535.1608647/1‑94 (MQ=60)
                                  ATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGA                                                             >  SRR4302535.1774237/1‑94 (MQ=60)
                                   TAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCA                                                            <  SRR4302535.2586977/94‑1 (MQ=60)
                                    AAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAA                                                           >  SRR4302535.3601129/1‑94 (MQ=60)
                                    AAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAA                                                           >  SRR4302535.4354886/1‑94 (MQ=60)
                                    AAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAA                                                           >  SRR4302535.3223566/1‑94 (MQ=60)
                                    AAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAA                                                           >  SRR4302535.4279116/1‑94 (MQ=60)
                                    AAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAA                                                           >  SRR4302535.1262963/1‑94 (MQ=60)
                                     AATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAAT                                                          <  SRR4302535.4474514/94‑1 (MQ=60)
                                     AATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAAT                                                          >  SRR4302535.2904387/1‑94 (MQ=60)
                                      ATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAATGCAATC                                                         <  SRR4302535.1448800/94‑1 (MQ=60)
                                      ATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATC                                                         >  SRR4302535.1740899/1‑94 (MQ=60)
                                      ATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGAAATC                                                         >  SRR4302535.1608447/1‑94 (MQ=60)
                                      ATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTAACGTCAAGGCAATC                                                         >  SRR4302535.2798421/1‑94 (MQ=60)
                                       TCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCC                                                        >  SRR4302535.4506870/1‑94 (MQ=60)
                                       TCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCC                                                        >  SRR4302535.1519878/1‑94 (MQ=60)
                                       TCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCC                                                        <  SRR4302535.1838124/94‑1 (MQ=60)
                                       TCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCC                                                        <  SRR4302535.533696/94‑1 (MQ=60)
                                        CTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCA                                                       >  SRR4302535.3290602/1‑94 (MQ=60)
                                         TAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAG                                                      <  SRR4302535.2693432/94‑1 (MQ=60)
                                         TAATGAAATTAATGGGTGTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAG                                                      <  SRR4302535.2576960/94‑1 (MQ=60)
                                          AATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGC                                                     <  SRR4302535.3813244/94‑1 (MQ=60)
                                          AATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGC                                                     >  SRR4302535.1245421/1‑94 (MQ=60)
                                           ATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCT                                                    >  SRR4302535.1965958/1‑94 (MQ=60)
                                           ATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCT                                                    >  SRR4302535.3801540/1‑94 (MQ=60)
                                            TGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTG                                                   <  SRR4302535.1215154/94‑1 (MQ=60)
                                            TGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTG                                                   <  SRR4302535.1318639/94‑1 (MQ=60)
                                             GAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGG                                                  <  SRR4302535.507796/94‑1 (MQ=60)
                                             GAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGG                                                  >  SRR4302535.922653/1‑94 (MQ=60)
                                              AAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGC                                                 >  SRR4302535.4025812/1‑94 (MQ=60)
                                              AAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGC                                                 >  SRR4302535.2278686/1‑94 (MQ=60)
                                              AAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGC                                                 >  SRR4302535.2243965/1‑94 (MQ=60)
                                               AATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCG                                                >  SRR4302535.686595/1‑94 (MQ=60)
                                               AATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCG                                                >  SRR4302535.2837873/1‑94 (MQ=60)
                                               AATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCG                                                <  SRR4302535.343217/94‑1 (MQ=60)
                                                  TAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACA                                             >  SRR4302535.2892610/1‑94 (MQ=60)
                                                   AATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAA                                            >  SRR4302535.1350182/1‑94 (MQ=60)
                                                    ATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAAT                                           <  SRR4302535.4591910/94‑1 (MQ=60)
                                                    ATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAAT                                           >  SRR4302535.3479585/1‑94 (MQ=60)
                                                      GGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCT                                         <  SRR4302535.2912833/94‑1 (MQ=60)
                                                       GGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTT                                        <  SRR4302535.1464909/94‑1 (MQ=60)
                                                        GTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTT                                       >  SRR4302535.1555779/1‑94 (MQ=60)
                                                        GTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTT                                       >  SRR4302535.750693/1‑94 (MQ=60)
                                                        GTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTT                                       <  SRR4302535.403112/94‑1 (MQ=60)
                                                         TTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCTATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTT                                      <  SRR4302535.2641916/94‑1 (MQ=60)
                                                            GGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCC                                   <  SRR4302535.4408791/94‑1 (MQ=60)
                                                            GGCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCC                                   <  SRR4302535.56440/94‑1 (MQ=60)
                                                             GCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCC                                  <  SRR4302535.4131342/94‑1 (MQ=60)
                                                             GCGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCC                                  <  SRR4302535.3606545/94‑1 (MQ=60)
                                                              CGTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCG                                 >  SRR4302535.2873996/1‑94 (MQ=60)
                                                               GTGCGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGA                                <  SRR4302535.4671463/94‑1 (MQ=60)
                                                                  CGTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATA                             >  SRR4302535.3501846/1‑94 (MQ=60)
                                                                   GTCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAG                            <  SRR4302535.1599527/94‑1 (MQ=60)
                                                                    TCGTGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGC                           <  SRR4302535.2112247/94‑1 (MQ=60)
                                                                       TGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCT                        <  SRR4302535.2632068/94‑1 (MQ=60)
                                                                       TGCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCT                        >  SRR4302535.3742920/1‑94 (MQ=60)
                                                                        GCATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTA                       <  SRR4302535.1002110/94‑1 (MQ=60)
                                                                         CATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAT                      <  SRR4302535.116944/94‑1 (MQ=60)
                                                                         CATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAT                      >  SRR4302535.2826345/1‑94 (MQ=60)
                                                                         CATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAT                      <  SRR4302535.598313/94‑1 (MQ=60)
                                                                         CATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGACAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTAT                      >  SRR4302535.4386963/1‑94 (MQ=60)
                                                                          ATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATT                     <  SRR4302535.1435060/94‑1 (MQ=60)
                                                                          ATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATT                     >  SRR4302535.4577144/1‑94 (MQ=60)
                                                                          ATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATT                     >  SRR4302535.2182834/1‑94 (MQ=60)
                                                                          ATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATT                     >  SRR4302535.3669233/1‑94 (MQ=60)
                                                                          ATGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGCCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATT                     >  SRR4302535.2348842/1‑94 (MQ=60)
                                                                           TGGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTC                    <  SRR4302535.560088/94‑1 (MQ=60)
                                                                            GGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCC                   <  SRR4302535.4432704/94‑1 (MQ=60)
                                                                            GGAGAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCC                   <  SRR4302535.670500/94‑1 (MQ=60)
                                                                               GAATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCT                <  SRR4302535.360839/94‑1 (MQ=60)
                                                                                AATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTT               <  SRR4302535.4719007/94‑1 (MQ=60)
                                                                                 ATATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTG              >  SRR4302535.4496922/1‑94 (MQ=60)
                                                                                  TATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGA             <  SRR4302535.1082381/94‑1 (MQ=60)
                                                                                   ATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAG            <  SRR4302535.1863983/94‑1 (MQ=60)
                                                                                   ATAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAG            <  SRR4302535.4166193/94‑1 (MQ=60)
                                                                                    TAGAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGA           >  SRR4302535.3257166/1‑94 (MQ=60)
                                                                                      GAGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAG         <  SRR4302535.1184055/94‑1 (MQ=60)
                                                                                       AGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGA        >  SRR4302535.541777/1‑94 (MQ=60)
                                                                                       AGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGA        <  SRR4302535.1335679/94‑1 (MQ=60)
                                                                                       AGAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGA        >  SRR4302535.797758/1‑94 (MQ=60)
                                                                                        GAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAG       <  SRR4302535.1362751/94‑1 (MQ=60)
                                                                                        GAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAG       <  SRR4302535.209513/94‑1 (MQ=60)
                                                                                        GAAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAG       <  SRR4302535.2609102/94‑1 (MQ=60)
                                                                                         AAGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGA      >  SRR4302535.2792616/1‑94 (MQ=60)
                                                                                          AGCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAA     >  SRR4302535.2490067/1‑94 (MQ=60)
                                                                                           GCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAAC    <  SRR4302535.447849/94‑1 (MQ=60)
                                                                                           GCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAAC    <  SRR4302535.1106035/94‑1 (MQ=60)
                                                                                           GCGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAAC    <  SRR4302535.4226449/94‑1 (MQ=60)
                                                                                            CGGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACA   <  SRR4302535.3045685/94‑1 (MQ=60)
                                                                                             GGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACAA  >  SRR4302535.2145492/1‑94 (MQ=60)
                                                                                             GGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACAA  <  SRR4302535.2009177/94‑1 (MQ=60)
                                                                                             GGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACAA  >  SRR4302535.443445/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCTACGATACCGATCGAGAAAGTGCGGGTTTTAAATAAATCTAATGAAATTAATGGGTTTGGCGTGCGTCGTGCATGGAGAATATAGAGAAGCAGTAACCCGATGCTGGTGACAATTACCGTCAAGGCAATCCAGCTGGCGACAATCTTTTCCCCGAATAGCTCTATTCCGCTTGAGAAGAGAACAA  >  NC_000913/3937430‑3937616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑