Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
1873 |
183.1 |
9376148 |
98.2% |
9207377 |
93.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,941,531 |
T→C |
noncoding (206/2906 nt) |
rrlC → |
23S ribosomal RNA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,941,531 | 0 | T | C | 100.0%
| 69.4
/ NA
| 32 | noncoding (206/2906 nt) | rrlC | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/19); total (13/19) |
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:3836607/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:3861947/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:3498492/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa > 2:443396/1‑93 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:4237807/93‑1 (MQ=14)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 2:2303046/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 2:1147355/93‑1 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 2:281260/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:3204957/93‑1 (MQ=17)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg < 1:2559745/93‑1 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 1:3285413/93‑1 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 2:2431534/93‑1 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 1:2651735/93‑1 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 1:2892595/93‑1 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 2:2237072/93‑1 (MQ=21)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg < 2:4524106/93‑1 (MQ=21)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg < 2:2219517/93‑1 (MQ=21)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg < 1:883849/93‑1 (MQ=21)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGACCAGAGACTGAATCAGTAtgcgtg > 1:4219839/1‑93 (MQ=11)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGTACGGGGACCAGCCCAGAGGCTGAATCAGTATgtgtgt > 2:4099540/1‑93 (MQ=11)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 2:1318811/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 2:4483009/93‑1 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:1068565/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:3606088/1‑93 (MQ=18)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 2:238424/93‑1 (MQ=21)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 1:3733748/93‑1 (MQ=21)
cTAAGCACCCCGAGGAAAAGAAAGCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 2:347709/93‑1 (MQ=255)
tAAGCACCTCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGACTGAATCAGTATGTGTGTTa > 2:4547493/1‑93 (MQ=255)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 2:3612549/93‑1 (MQ=21)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa > 1:2954095/1‑93 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa > 1:3940251/1‑93 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa > 1:766499/1‑93 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa > 2:1771247/1‑93 (MQ=18)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg < 2:2238007/93‑1 (MQ=18)
|
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
