Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
1696 |
279.3 |
15874187 |
97.8% |
15524954 |
93.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,941,531 |
T→C |
noncoding (206/2906 nt) |
rrlC → |
23S ribosomal RNA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,941,531 | 0 | T | C | 97.9%
| 99.8
/ ‑4.5
| 48 | noncoding (206/2906 nt) | rrlC | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/24); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 |
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:1851865/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:7522493/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:5954010/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:5506873/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:4367733/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:6458886/93‑1 (MQ=14)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:621103/93‑1 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 1:6101994/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:1656300/93‑1 (MQ=17)
agaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGGGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:7442242/91‑1 (MQ=255)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg > 2:798917/1‑93 (MQ=18)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg > 1:3095720/1‑93 (MQ=18)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg > 1:2344222/1‑93 (MQ=18)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg > 2:7583352/1‑93 (MQ=18)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATAAGTAtg > 1:2851860/1‑93 (MQ=255)
aaCATCTAAGCACCCCGAGGAAAAGAATTCAACCGAGATACTGCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 2:3741570/93‑1 (MQ=11)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt > 2:2270176/1‑93 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt > 2:3918761/1‑93 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGGAtgg > 2:4479693/1‑92 (MQ=9)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGATCGGGGAGCAGCCCCGAGCCTGAATCAGTAtgag > 1:7629565/1‑91 (MQ=11)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 1:6976326/93‑1 (MQ=18)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 1:5214325/93‑1 (MQ=18)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 2:7833538/93‑1 (MQ=18)
cATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt > 1:3164755/1‑93 (MQ=33)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 1:7464898/93‑1 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt > 2:6197501/1‑93 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 2:631855/93‑1 (MQ=21)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt > 2:7875497/1‑93 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt > 2:1802127/1‑93 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 1:6626623/93‑1 (MQ=21)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgg > 1:4118723/1‑92 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGGAtgtgt > 2:6197502/1‑93 (MQ=16)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 1:1683639/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 2:3581473/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg < 2:6806205/93‑1 (MQ=21)
tCTAAGCACCGCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:6515429/93‑1 (MQ=255)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCTAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:5412779/93‑1 (MQ=16)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:7931021/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 2:2179839/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 2:3081410/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:6239684/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:4768596/93‑1 (MQ=21)
cTAAGCCCCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGACCGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 1:4330754/93‑1 (MQ=255)
cTAAGCCCCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAAC‑GGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 1:1842370/93‑1 (MQ=11)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 1:7898419/93‑1 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 1:5810601/93‑1 (MQ=21)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 1:1878437/93‑1 (MQ=21)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa > 2:2893921/1‑93 (MQ=18)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGGGCAGCCCCGAGCCTGAATCAGTATGGGTGTTAg > 1:1964436/1‑93 (MQ=11)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg < 2:3600202/93‑1 (MQ=21)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg < 2:3103645/93‑1 (MQ=21)
|
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
