Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,351	+C	coding (1/600 nt) coding (240/240 nt)	yifDA ← yifDA ←	transcription regulator transcription regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,350	1	.	C	99.6%	743.2 / ‑4.2	233	coding (2/600 nt)	yifDA	transcription regulator
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (122/110); total (122/111)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 252 total aligned reads displayed.

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945260‑3945437

aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACc‑t‑cc                                                                                       >  2:1790386/1‑92 (MQ=255)

aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACc‑t‑cc                                                                                       >  2:2268273/1‑92 (MQ=255)

  gCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACc‑t‑cctt                                                                                     >  2:3969978/1‑90 (MQ=255)

     tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGGATGCGCCACC‑TCCTTACg                                                                                   >  1:3218273/1‑93 (MQ=255)

      cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACgg                                                                                  <  1:2475366/93‑1 (MQ=255)

      cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACgg                                                                                  >  2:1512873/1‑93 (MQ=255)

       ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACggg                                                                                 >  1:1819268/1‑93 (MQ=255)

       ccAGCCACTGATTAAGCATACATTCACACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGCTATGCGCCACC‑TCCTTACggc                                                                                 >  2:4357622/1‑92 (MQ=255)

        cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGggc                                                                                >  1:2387194/1‑93 (MQ=255)

         aGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGggcg                                                                               >  2:2919992/1‑93 (MQ=255)

         aGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGggcg                                                                               >  2:2004069/1‑93 (MQ=255)

          gCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGggcgg                                                                              >  2:752908/1‑93 (MQ=255)

             aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCt                                                                           >  1:3595407/1‑93 (MQ=255)

             aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCt                                                                           >  2:3846807/1‑93 (MQ=255)

             aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCt                                                                           >  1:1499139/1‑93 (MQ=255)

               tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGc                                                                         >  2:1989987/1‑93 (MQ=255)

               tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGc                                                                         >  2:1178494/1‑93 (MQ=255)

               tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGc                                                                         >  1:2620604/1‑93 (MQ=255)

               tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGc                                                                         >  2:4275447/1‑93 (MQ=255)

                gATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGcc                                                                        >  2:803028/1‑93 (MQ=255)

                gATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGcc                                                                        >  1:4401533/1‑93 (MQ=255)

                  ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAg                                                                      <  1:1414145/93‑1 (MQ=255)

                    aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAggg                                                                    >  2:3882203/1‑92 (MQ=255)

                        aTACATTCCCACAACGAGGCGCTGTCACCGATAGAAACCTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTc                                                                <  2:349010/93‑1 (MQ=255)

                        aTACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTc                                                                >  2:2120841/1‑93 (MQ=255)

                         tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               >  2:225561/1‑93 (MQ=255)

                          aCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCAt                                                              <  2:266232/93‑1 (MQ=255)

                            aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            >  2:2793439/1‑93 (MQ=255)

                            aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            <  1:4579081/93‑1 (MQ=255)

                             ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           >  2:4352915/1‑93 (MQ=255)

                             ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           <  2:3748497/93‑1 (MQ=255)

                                ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  2:4093167/1‑93 (MQ=255)

                                ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  1:3731757/1‑93 (MQ=255)

                                ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  2:2757003/1‑93 (MQ=255)

                                ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  2:2538731/1‑93 (MQ=255)

                                 cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  1:1274509/1‑93 (MQ=255)

                                 cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  2:727719/1‑93 (MQ=255)

                                 cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       <  2:3225998/93‑1 (MQ=255)

                                   caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:1743365/93‑1 (MQ=255)

                                   caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:2438007/93‑1 (MQ=255)

                                   caACGAGGAGCTGGCACCGATAGAAAACTCGTTATGTCGTGTGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     >  2:4547939/1‑93 (MQ=255)

                                    aaCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt                                                    <  2:1242365/93‑1 (MQ=255)

                                    aaCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt                                                    <  2:3550157/93‑1 (MQ=255)

                                     aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  1:4463886/1‑93 (MQ=255)

                                     aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  1:1058019/1‑93 (MQ=255)

                                      cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  <  2:2994378/93‑1 (MQ=255)

                                        aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                >  1:1597721/1‑93 (MQ=255)

                                          gcgcTGGCACCAATAGAAACCTCGTTATTTCGTGAGTTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTC‑TGAGCGTTTCTGCATAAg                                             <  1:3556067/93‑1 (MQ=255)

                                            gcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgg                                            >  1:3339238/1‑93 (MQ=255)

                                             cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           >  2:4487519/1‑93 (MQ=255)

                                              tGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa                                          <  1:3288337/93‑1 (MQ=255)

                                               ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         <  1:2036777/93‑1 (MQ=255)

                                               ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         >  1:2368217/1‑93 (MQ=255)

                                               ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         <  1:273356/93‑1 (MQ=255)

                                                 cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  2:3034295/93‑1 (MQ=255)

                                                 cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  2:1303491/93‑1 (MQ=255)

                                                 cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  1:659778/93‑1 (MQ=255)

                                                 cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  1:3373271/93‑1 (MQ=255)

                                                  aCCGATAGAAAACTCGTTATGTCGTGAGGGATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtag                                      >  1:3004939/1‑93 (MQ=255)

                                                   ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     <  2:1363430/93‑1 (MQ=255)

                                                   ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  1:2756562/1‑93 (MQ=255)

                                                   ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  2:3306824/1‑93 (MQ=255)

                                                    cGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt                                    <  1:544636/93‑1 (MQ=255)

                                                     gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   >  1:758189/1‑93 (MQ=255)

                                                      aTAGAAAACTGGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  >  2:2868924/1‑93 (MQ=255)

                                                      aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  <  2:1963904/93‑1 (MQ=255)

                                                      aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  >  2:1563052/1‑93 (MQ=255)

                                                        aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCGC‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                <  1:1915289/93‑1 (MQ=255)

                                                        aGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  2:4265310/1‑93 (MQ=255)

                                                        aGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                <  1:4256990/93‑1 (MQ=255)

                                                        aGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTGCTGCATAAGGCAGTAGTTTTTc                                >  1:973111/1‑93 (MQ=255)

                                                         gAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               <  1:3627080/93‑1 (MQ=255)

                                                          aaaaCTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAc                              >  1:4051393/1‑93 (MQ=255)

                                                            aaCTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg                            <  1:3571795/93‑1 (MQ=255)

                                                            aaCTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg                            >  1:2503060/1‑93 (MQ=255)

                                                             aCTCGTTATGTCGTGAGGTATGCGCCGCC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           <  1:3811489/93‑1 (MQ=255)

                                                              cTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc                          >  1:1687071/1‑93 (MQ=255)

                                                               tCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCa                         >  1:4668285/1‑93 (MQ=255)

                                                                  ttATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg                      <  2:4660758/93‑1 (MQ=255)

                                                                   tATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg                     >  1:1927747/1‑93 (MQ=255)

                                                                   tATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg                     >  1:2106137/1‑93 (MQ=255)

                                                                    aTGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  1:3678365/93‑1 (MQ=255)

                                                                    aTGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  1:2311883/93‑1 (MQ=255)

                                                                     tGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   <  1:3451376/93‑1 (MQ=255)

                                                                        cGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  1:519434/93‑1 (MQ=255)

                                                                          tGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACg              >  2:777450/1‑93 (MQ=255)

                                                                           gAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACgg             >  1:2868537/1‑93 (MQ=255)

                                                                            aGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTCGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:1868794/93‑1 (MQ=255)

                                                                            aGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACTGa            >  2:707057/1‑93 (MQ=255)

                                                                            aGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:873606/93‑1 (MQ=255)

                                                                             ggTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           <  1:3779513/93‑1 (MQ=255)

                                                                             ggTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           <  1:385988/93‑1 (MQ=255)

                                                                             ggTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           >  1:4297535/1‑93 (MQ=255)

                                                                             ggTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           >  2:1657064/1‑93 (MQ=255)

                                                                               tATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         >  2:3460543/1‑93 (MQ=255)

                                                                                  gcgcCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  1:1148340/93‑1 (MQ=255)

                                                                                    gcCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGttt    <  1:2155519/93‑1 (MQ=255)

                                                                                     ccACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   >  2:4414073/1‑93 (MQ=255)

                                                                                     ccACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   <  2:17508/93‑1 (MQ=255)

                                                                                      cACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCt  >  1:4094139/1‑93 (MQ=255)

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945260‑3945437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945260‑3945444

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CCT                                                                                             <  SRR4302535.2844489/94‑1 (MQ=60)

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CCT                                                                                             <  SRR4302535.3259403/94‑1 (MQ=60)

   CGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTAC                                                                                           <  SRR4302535.3765520/94‑1 (MQ=60)

   CGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTAC                                                                                           <  SRR4302535.1931756/94‑1 (MQ=60)

    GTCCCAGCCACTGATTAAGCATACATTCCCACCACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑Ccttgcg                                                                                         >  SRR4302535.1004671/1‑88 (MQ=60)

    GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACG                                                                                          <  SRR4302535.784222/94‑1 (MQ=60)

    GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACG                                                                                          <  SRR4302535.2427270/94‑1 (MQ=60)

    GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGGATGCGCCACC‑TCCTTACG                                                                                          >  SRR4302535.3240563/1‑94 (MQ=60)

    GCCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCGCC‑TCCTTACG                                                                                          <  SRR4302535.1699036/94‑1 (MQ=60)

     TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGG                                                                                         <  SRR4302535.2920770/94‑1 (MQ=60)

      CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGG                                                                                        <  SRR4302535.2493220/94‑1 (MQ=60)

      CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGG                                                                                        <  SRR4302535.3344443/94‑1 (MQ=60)

      CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGG                                                                                        >  SRR4302535.1832413/1‑94 (MQ=60)

       CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGC                                                                                       >  SRR4302535.1683905/1‑94 (MQ=60)

       CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGC                                                                                       <  SRR4302535.1296645/94‑1 (MQ=60)

       CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGC                                                                                       >  SRR4302535.2404353/1‑94 (MQ=60)

        CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCG                                                                                      >  SRR4302535.2672444/1‑94 (MQ=60)

        CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCG                                                                                      >  SRR4302535.1892602/1‑94 (MQ=60)

           CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCC                                                                                   >  SRR4302535.260888/1‑94 (MQ=60)

            CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCT                                                                                  >  SRR4302535.1509924/1‑94 (MQ=60)

            CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCT                                                                                  >  SRR4302535.3620356/1‑94 (MQ=60)

            CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCT                                                                                  >  SRR4302535.3112537/1‑94 (MQ=60)

             ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTG                                                                                 <  SRR4302535.4405981/94‑1 (MQ=60)

             ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTG                                                                                 >  SRR4302535.2515231/1‑94 (MQ=60)

             ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTG                                                                                 >  SRR4302535.3985647/1‑94 (MQ=60)

              CTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGC                                                                                >  SRR4302535.2639392/1‑94 (MQ=60)

              CTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGC                                                                                >  SRR4302535.3379864/1‑94 (MQ=60)

               TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCC                                                                               >  SRR4302535.2143824/1‑94 (MQ=60)

               TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCC                                                                               >  SRR4302535.4431652/1‑94 (MQ=60)

               TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCC                                                                               >  SRR4302535.1443853/1‑94 (MQ=60)

                GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCA                                                                              >  SRR4302535.216987/1‑94 (MQ=60)

                  TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302535.1424241/94‑1 (MQ=60)

                  TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302535.4435612/94‑1 (MQ=60)

                     AGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGC                                                                         >  SRR4302535.4294050/1‑94 (MQ=60)

                     AGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGC                                                                         >  SRR4302535.569138/1‑94 (MQ=60)

                     AGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGC                                                                         >  SRR4302535.3332526/1‑94 (MQ=60)

                       CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTC                                                                       >  SRR4302535.3595933/1‑94 (MQ=60)

                       CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTC                                                                       >  SRR4302535.536365/1‑94 (MQ=60)

                       CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTC                                                                       >  SRR4302535.1607637/1‑94 (MQ=60)

                         TACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302535.2758202/94‑1 (MQ=60)

                            ATTCCCACAACGAGGCGCTGGCACCGATCGAAAACTCGTTATGCCGTGAGGAATGCGCCACC‑TACTTCCGGGCGGCCTCCCAGGTGCTCAcgtc                                                                  >  SRR4302535.1513113/1‑90 (MQ=60)

                            ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302535.4610273/94‑1 (MQ=60)

                            ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302535.1079317/94‑1 (MQ=60)

                             TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                 >  SRR4302535.2054145/1‑94 (MQ=60)

                               CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               >  SRR4302535.3757596/1‑94 (MQ=60)

                                CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              >  SRR4302535.4373623/1‑94 (MQ=60)

                                CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              >  SRR4302535.1283607/1‑94 (MQ=60)

                                 CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCGCC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                             <  SRR4302535.507693/94‑1 (MQ=60)

                                 CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                             <  SRR4302535.4696613/94‑1 (MQ=60)

                                 CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                             >  SRR4302535.1180205/1‑94 (MQ=60)

                                  ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGACACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTT                                                            >  SRR4302535.644792/1‑94 (MQ=60)

                                   CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           >  SRR4302535.241548/1‑94 (MQ=60)

                                   CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302535.343137/94‑1 (MQ=60)

                                   CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302535.1755979/94‑1 (MQ=60)

                                   CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           >  SRR4302535.3418440/1‑94 (MQ=60)

                                    AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302535.2890609/1‑94 (MQ=60)

                                    AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302535.4494503/1‑94 (MQ=60)

                                    AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302535.1065545/1‑94 (MQ=60)

                                      CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                        >  SRR4302535.3596159/1‑94 (MQ=60)

                                       GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                       >  SRR4302535.1609243/1‑94 (MQ=60)

                                       GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                       >  SRR4302535.3661930/1‑94 (MQ=60)

                                         GGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAA                                                     <  SRR4302535.1912456/94‑1 (MQ=60)

                                         GGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAA                                                     >  SRR4302535.2290002/1‑94 (MQ=60)

                                          GCGCTGGCACCAATAGAAACCTCGTTATTTCGTGAGTTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTC‑TGAGCGTTTCTGCATAAGG                                                   <  SRR4302535.3580732/94‑1 (MQ=60)

                                           CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   <  SRR4302535.2396928/94‑1 (MQ=60)

                                           CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   >  SRR4302535.3362498/1‑94 (MQ=60)

                                            GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                  >  SRR4302535.2546034/1‑94 (MQ=60)

                                            GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                  <  SRR4302535.2352057/94‑1 (MQ=60)

                                             CTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCA                                                 <  SRR4302535.3835646/94‑1 (MQ=60)

                                              TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                >  SRR4302535.4597812/1‑94 (MQ=60)

                                              TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302535.1159421/94‑1 (MQ=60)

                                              TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302535.3311200/94‑1 (MQ=60)

                                              TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                >  SRR4302535.2385218/1‑94 (MQ=60)

                                              TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302535.164065/94‑1 (MQ=60)

                                               GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               <  SRR4302535.2051444/94‑1 (MQ=60)

                                               GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               <  SRR4302535.275261/94‑1 (MQ=60)

                                                GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              <  SRR4302535.1737658/94‑1 (MQ=60)

                                                GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              >  SRR4302535.3647199/1‑94 (MQ=60)

                                                 CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302535.664381/94‑1 (MQ=60)

                                                 CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302535.3396734/94‑1 (MQ=60)

                                                 CACCGATAGAAAACTCGTTATGTCGTGAGGGATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             >  SRR4302535.3025999/1‑94 (MQ=60)

                                                  ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            >  SRR4302535.2776210/1‑94 (MQ=60)

                                                    CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          <  SRR4302535.548442/94‑1 (MQ=60)

                                                    CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302535.763532/1‑94 (MQ=60)

                                                     GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                         <  SRR4302535.685073/94‑1 (MQ=60)

                                                      ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        <  SRR4302535.4131112/94‑1 (MQ=60)

                                                       TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTGCTGCATAAGGCAGTAGTTTTTC                                       >  SRR4302535.980016/1‑94 (MQ=60)

                                                        AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCGC‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      <  SRR4302535.1929091/94‑1 (MQ=60)

                                                        AGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302535.754650/1‑94 (MQ=60)

                                                        AGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302535.472120/1‑94 (MQ=60)

                                                        AGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      <  SRR4302535.4286157/94‑1 (MQ=60)

                                                         GAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                     <  SRR4302535.3652218/94‑1 (MQ=60)

                                                         GAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                     >  SRR4302535.4113000/1‑94 (MQ=60)

                                                         GAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                     >  SRR4302535.4079130/1‑94 (MQ=60)

                                                           AAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   <  SRR4302535.3620028/94‑1 (MQ=60)

                                                           AAACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302535.2521109/1‑94 (MQ=60)

                                                            AACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                  >  SRR4302535.1844697/1‑94 (MQ=60)

                                                            AACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                  <  SRR4302535.3596563/94‑1 (MQ=60)

                                                             ACTCGTTATGTCGTGAGGTATGCGCCGCC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 <  SRR4302535.3837785/94‑1 (MQ=60)

                                                             ACTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 >  SRR4302535.1699283/1‑94 (MQ=60)

                                                              CTCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCA                                >  SRR4302535.4699828/1‑94 (MQ=60)

                                                               TCGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAG                               <  SRR4302535.84343/94‑1 (MQ=60)

                                                                CGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                              <  SRR4302535.4571514/94‑1 (MQ=60)

                                                                CGTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTACATAAGGCAGTAGTTTTTCACCGGCAGC                              <  SRR4302535.978007/94‑1 (MQ=60)

                                                                 GTTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302535.2776134/94‑1 (MQ=60)

                                                                  TTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            >  SRR4302535.1941639/1‑94 (MQ=60)

                                                                  TTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            >  SRR4302535.2121329/1‑94 (MQ=60)

                                                                  TTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            <  SRR4302535.934503/94‑1 (MQ=60)

                                                                  TTATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            >  SRR4302535.3820213/1‑94 (MQ=60)

                                                                   TATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGT                           <  SRR4302535.4078240/94‑1 (MQ=60)

                                                                    ATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302535.3703825/94‑1 (MQ=60)

                                                                    ATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          >  SRR4302535.196083/1‑94 (MQ=60)

                                                                    ATGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302535.2328434/94‑1 (MQ=60)

                                                                     TGTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                         <  SRR4302535.3475366/94‑1 (MQ=60)

                                                                      GTCGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAA                        >  SRR4302535.3714859/1‑94 (MQ=60)

                                                                        CGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302535.523068/94‑1 (MQ=60)

                                                                        CGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302535.2010929/1‑94 (MQ=60)

                                                                        CGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302535.386062/1‑94 (MQ=60)

                                                                        CGTGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302535.3633573/94‑1 (MQ=60)

                                                                          TGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    >  SRR4302535.2888855/1‑94 (MQ=60)

                                                                          TGAGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    <  SRR4302535.2437890/94‑1 (MQ=60)

                                                                            AGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTCGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302535.1882265/94‑1 (MQ=60)

                                                                            AGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  >  SRR4302535.4326946/1‑94 (MQ=60)

                                                                            AGGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302535.879797/94‑1 (MQ=60)

                                                                             GGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302535.2348707/94‑1 (MQ=60)

                                                                             GGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302535.3555818/94‑1 (MQ=60)

                                                                             GGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302535.388670/94‑1 (MQ=60)

                                                                             GGTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302535.3805599/94‑1 (MQ=60)

                                                                              GTATGCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                >  SRR4302535.374267/1‑94 (MQ=60)

                                                                                  GCGCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302535.1156520/94‑1 (MQ=60)

                                                                                    GCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          <  SRR4302535.2171008/94‑1 (MQ=60)

                                                                                    GCCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          <  SRR4302535.4237080/94‑1 (MQ=60)

                                                                                     CCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         <  SRR4302535.3220541/94‑1 (MQ=60)

                                                                                     CCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302535.1810617/1‑94 (MQ=60)

                                                                                     CCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302535.1809728/1‑94 (MQ=60)

                                                                                     CCACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302535.4122157/1‑94 (MQ=60)

                                                                                      CACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        <  SRR4302535.1455164/94‑1 (MQ=60)

                                                                                      CACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302535.4033998/1‑94 (MQ=60)

                                                                                      CACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302535.1808868/1‑94 (MQ=60)

                                                                                      CACC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302535.4252780/1‑94 (MQ=60)

                                                                                        CC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      >  SRR4302535.1240920/1‑94 (MQ=60)

                                                                                        CC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      <  SRR4302535.3678923/94‑1 (MQ=60)

                                                                                        CC‑TCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      <  SRR4302535.1023000/94‑1 (MQ=60)

                                                                                          ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     <  SRR4302535.4126480/91‑1 (MQ=60)

                                                                                          ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302535.2341926/4‑94 (MQ=60)

                                                                                             CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  SRR4302535.2027677/1‑94 (MQ=60)

AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACC‑T‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945260‑3945444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`