Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,351	+C	coding (1/600 nt) coding (240/240 nt)	yifDA ← yifDA ←	transcription regulator transcription regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,350	1	.	C	99.8%	1263.8 / ‑4.5	397	coding (2/600 nt)	yifDA	transcription regulator
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (189/207); total (189/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 435 total aligned reads displayed.

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

aCAGGCGTCCCATCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCTTTATGTCGTGAGGTATGCGCCACct                                                                                          <  2:3251620/93‑1 (MQ=255)

aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct                                                                                          >  1:5636688/1‑93 (MQ=255)

 cAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑c                                                                                        <  2:7668803/93‑1 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       <  2:7718217/93‑2 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cct                                                                                      <  2:823339/93‑3 (MQ=255)

     cGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTTGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCctcct‑cctta                                                                                    <  2:6618151/93‑5 (MQ=255)

     cGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cctta                                                                                    <  2:1670704/93‑5 (MQ=255)

     cGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cctta                                                                                    <  1:3150989/93‑5 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg                                                                                   >  1:4911927/1‑93 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg                                                                                   <  2:2366104/93‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg                                                                                  <  1:7002238/93‑1 (MQ=255)

         ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg                                                                                 >  1:1870019/1‑93 (MQ=255)

          cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggc                                                                                >  1:1237577/1‑93 (MQ=255)

          cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGCATGCGCCACCTCCTTACGggc                                                                                <  2:2287714/93‑1 (MQ=255)

           aGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggcg                                                                               <  2:7885956/93‑1 (MQ=255)

            gCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggcgc                                                                              >  2:3621281/1‑92 (MQ=255)

             ccACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggcggc                                                                             >  1:1002613/1‑93 (MQ=255)

               aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt                                                                           <  1:5401569/93‑1 (MQ=255)

                  gATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGcc                                                                        >  1:437338/1‑93 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:3489375/93‑1 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:7666882/93‑1 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      >  2:1281682/1‑93 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  2:2609527/93‑1 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:4268369/93‑1 (MQ=255)

                    atAAGCATACATTCCCACAACGAAGCGCTTGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:6663121/92‑1 (MQ=255)

                       aGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTg                                                                   >  2:1564184/1‑93 (MQ=255)

                          aTACATTCCCACAACTAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTc                                                                <  2:1905398/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  2:7027379/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  1:5204306/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  1:2313440/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  2:5801232/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCCGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               >  2:6534519/1‑93 (MQ=255)

                            aCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAt                                                              <  2:2143/93‑1 (MQ=255)

                             cATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATg                                                             >  1:3592576/1‑93 (MQ=255)

                             cATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATg                                                             >  1:1994852/1‑93 (MQ=255)

                              atccccACAACGAGGCGCTGGCAACGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            <  2:4364172/90‑1 (MQ=255)

                              aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            >  1:2624168/1‑93 (MQ=255)

                               ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           >  2:7447262/1‑93 (MQ=255)

                               ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           >  1:901001/1‑93 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACGTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  1:2068034/1‑93 (MQ=255)

                                   cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  2:4451638/1‑93 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      >  2:5533133/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:6309886/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:873079/93‑1 (MQ=255)

                                      aaCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt                                                    <  1:6141632/93‑1 (MQ=255)

                                       aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  1:737994/1‑93 (MQ=255)

                                       aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  2:2851532/1‑93 (MQ=255)

                                        cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTATGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  <  1:6838467/93‑1 (MQ=255)

                                         gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCa                                                 >  1:6491077/1‑93 (MQ=255)

                                         gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCa                                                 >  2:2012225/1‑93 (MQ=255)

                                           ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa                                               <  2:185310/93‑1 (MQ=255)

                                              gcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgg                                            <  1:7073583/93‑1 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  1:1626477/93‑1 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  2:1741795/93‑1 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   >  2:6361585/1‑93 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   <  2:6668996/93‑1 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   >  2:2083621/1‑93 (MQ=255)

                                                        aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  >  2:5391537/1‑93 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  1:3967741/1‑93 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  1:1382426/1‑93 (MQ=255)

                                                          aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  1:3032449/1‑93 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               <  2:4157905/93‑1 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGTAGTAGTTTTTCa                               >  2:3947773/1‑93 (MQ=255)

                                                            aaaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAc                              >  1:3828042/1‑93 (MQ=255)

                                                             aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcc                             <  1:7806310/93‑1 (MQ=255)

                                                             aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcc                             >  1:1522809/1‑93 (MQ=255)

                                                              aaCTCGTTATGTCGTGATGTATGCGCCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCCCCg                            <  2:730024/93‑1 (MQ=255)

                                                               aCTCGTTATGTCGTGAGGCATGCGCCACCTCCTTACGGGCGGCCTGCCATGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           >  2:7581282/1‑93 (MQ=255)

                                                                cTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCCCCGGc                          <  2:3785524/93‑1 (MQ=255)

                                                                cTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc                          <  2:6923122/93‑1 (MQ=255)

                                                                 tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCa                         >  2:2989869/1‑93 (MQ=255)

                                                                  cGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAg                        >  2:2717782/1‑93 (MQ=255)

                                                                  cGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAg                        <  1:6108120/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCTGTAGTTTTTCACCGGCAGc                       >  2:4899851/1‑93 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  1:7547992/93‑1 (MQ=255)

                                                                    ttATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg                      <  2:6224223/93‑1 (MQ=255)

                                                                    ttATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg                      >  2:6944641/1‑93 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    >  1:3404241/1‑93 (MQ=255)

                                                                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   >  1:3644906/1‑93 (MQ=255)

                                                                         tCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaa                 <  1:2862361/93‑1 (MQ=255)

                                                                          cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  1:972536/93‑1 (MQ=255)

                                                                          cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  1:4549962/93‑1 (MQ=255)

                                                                           gTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAc               >  1:5591241/1‑93 (MQ=255)

                                                                             gAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACgg             >  1:4803369/1‑93 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  2:5555278/93‑1 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:2519592/93‑1 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  2:254354/93‑1 (MQ=255)

                                                                               ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           >  1:5889484/1‑93 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  1:4194493/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  1:6735192/93‑1 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         <  2:454890/93‑1 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         >  1:5057270/1‑93 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         >  2:6024335/1‑93 (MQ=255)

                                                                                   tGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTg       >  1:5518933/1‑93 (MQ=255)

                                                                                   tGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTg       >  2:532336/1‑93 (MQ=255)

                                                                                    gcgcCTCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  1:2735241/93‑1 (MQ=255)

                                                                                     cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGtt     <  1:2925887/93‑1 (MQ=255)

                                                                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGttt    <  1:7723730/93‑1 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   <  2:4721490/93‑1 (MQ=255)

                                                                                        cACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCt  <  1:5731929/93‑1 (MQ=255)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945258‑3945444

 CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CC                                                                                              >  SRR4302543.5571496/1‑94 (MQ=60)

   AGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑Cctt                                                                                            <  SRR4302543.11160/94‑4 (MQ=60)

   AGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑Cctt                                                                                            <  SRR4302543.5668318/94‑4 (MQ=60)

     CGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTAC                                                                                           <  SRR4302543.2773931/94‑1 (MQ=60)

     CGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTAC                                                                                           <  SRR4302543.3179341/94‑1 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                          >  SRR4302543.4956694/1‑94 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                          >  SRR4302543.1550834/1‑94 (MQ=60)

       TCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGG                                                                                         <  SRR4302543.3474927/94‑1 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                        >  SRR4302543.1886819/1‑94 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                        <  SRR4302543.7068671/94‑1 (MQ=60)

         CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGC                                                                                       >  SRR4302543.1248712/1‑94 (MQ=60)

         CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGC                                                                                       >  SRR4302543.6671052/1‑94 (MQ=60)

          CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCG                                                                                      <  SRR4302543.5797050/94‑1 (MQ=60)

          CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCG                                                                                      <  SRR4302543.6530815/94‑1 (MQ=60)

          CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCG                                                                                      <  SRR4302543.7615332/94‑1 (MQ=60)

           AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGG                                                                                     <  SRR4302543.6859884/94‑1 (MQ=60)

            GCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGC                                                                                    >  SRR4302543.1011474/1‑94 (MQ=60)

             CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCC                                                                                   <  SRR4302543.5108736/94‑1 (MQ=60)

             CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCC                                                                                   >  SRR4302543.6877918/1‑94 (MQ=60)

              ncnTGATNAAGCATACATTCNNANAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                  <  SRR4302543.2312928/91‑1 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                  >  SRR4302543.3126765/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCGACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302543.7736319/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 <  SRR4302543.3168748/94‑1 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 <  SRR4302543.5450682/94‑1 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302543.3490278/1‑94 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 <  SRR4302543.877564/94‑1 (MQ=60)

               ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302543.4895767/1‑94 (MQ=60)

                CTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGC                                                                                <  SRR4302543.7469835/94‑1 (MQ=60)

                 TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCC                                                                               >  SRR4302543.441436/1‑94 (MQ=60)

                 TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCC                                                                               >  SRR4302543.1123659/1‑94 (MQ=60)

                 TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCC                                                                               <  SRR4302543.4899225/94‑1 (MQ=60)

                 TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCC                                                                               <  SRR4302543.1971246/94‑1 (MQ=60)

                  GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCA                                                                              <  SRR4302543.1373912/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.7739544/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.2927188/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.4479430/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            >  SRR4302543.6123583/1‑94 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            >  SRR4302543.4339057/1‑94 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.3520608/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.484256/94‑1 (MQ=60)

                    ATAAGCATACATTCCCACAACGAAGCGCTTGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302543.6726030/94‑1 (MQ=60)

                     TAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGT                                                                           <  SRR4302543.6456218/94‑1 (MQ=60)

                      AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                          >  SRR4302543.2658561/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                        >  SRR4302543.2880491/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                        <  SRR4302543.7773402/94‑1 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                       >  SRR4302543.975350/1‑94 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCACGTGATC                                                                       >  SRR4302543.2057207/1‑94 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      >  SRR4302543.6360090/1‑94 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      >  SRR4302543.215245/1‑94 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      <  SRR4302543.3676072/94‑1 (MQ=60)

                           TACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302543.5251220/94‑1 (MQ=60)

                           TACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302543.2047875/94‑1 (MQ=60)

                           TACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302543.2334481/94‑1 (MQ=60)

                           TACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAT                                                                     <  SRR4302543.3955796/94‑1 (MQ=60)

                            ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    <  SRR4302543.3078506/94‑1 (MQ=60)

                            ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    >  SRR4302543.3624288/1‑94 (MQ=60)

                            ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    >  SRR4302543.2012740/1‑94 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302543.4800755/1‑94 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302543.5360781/1‑94 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302543.1177993/94‑1 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  >  SRR4302543.4171560/1‑94 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  >  SRR4302543.4252004/1‑94 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302543.7939388/94‑1 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  >  SRR4302543.233565/1‑94 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  >  SRR4302543.908950/1‑94 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302543.2553267/94‑1 (MQ=60)

                               TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                 <  SRR4302543.4286094/94‑1 (MQ=60)

                                 CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACGTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               >  SRR4302543.2086521/1‑94 (MQ=60)

                                 CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               <  SRR4302543.2700601/94‑1 (MQ=60)

                                  CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              >  SRR4302543.5569410/1‑94 (MQ=60)

                                  CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              >  SRR4302543.5569412/1‑94 (MQ=60)

                                    ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTC                                                            >  SRR4302543.6767079/1‑94 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302543.4327032/94‑1 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302543.2576365/94‑1 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302543.2661478/94‑1 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           >  SRR4302543.3550878/1‑94 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302543.6369228/94‑1 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302543.5503929/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          <  SRR4302543.6199803/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302543.744597/1‑94 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          <  SRR4302543.1488983/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302543.3348818/1‑94 (MQ=60)

                                       ACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGC                                                         <  SRR4302543.3751391/94‑1 (MQ=60)

                                        CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                        >  SRR4302543.6552228/1‑94 (MQ=60)

                                           GGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAA                                                     <  SRR4302543.4490483/94‑1 (MQ=60)

                                            GCGCGGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                    <  SRR4302543.327335/94‑1 (MQ=60)

                                             CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   >  SRR4302543.977765/1‑94 (MQ=60)

                                             CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   <  SRR4302543.3675488/94‑1 (MQ=60)

                                             CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   <  SRR4302543.1473374/94‑1 (MQ=60)

                                              GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                  <  SRR4302543.4014305/94‑1 (MQ=60)

                                              GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                  <  SRR4302543.7142267/94‑1 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302543.6364803/94‑1 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302543.5623524/94‑1 (MQ=60)

                                                 GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               >  SRR4302543.2621092/1‑94 (MQ=60)

                                                   CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302543.778809/94‑1 (MQ=60)

                                                   CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302543.1640968/94‑1 (MQ=60)

                                                   CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             >  SRR4302543.5001485/1‑94 (MQ=60)

                                                    ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            <  SRR4302543.5487098/94‑1 (MQ=60)

                                                    ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            >  SRR4302543.1607718/1‑94 (MQ=60)

                                                    ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGCAGT                                            >  SRR4302543.5776837/1‑94 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                           <  SRR4302543.7275157/94‑1 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGCGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302543.3856931/1‑94 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302543.3050107/1‑94 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302543.2919554/1‑94 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302543.7552189/1‑94 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          >  SRR4302543.4670875/1‑94 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                         >  SRR4302543.4996459/1‑94 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                         <  SRR4302543.7629148/94‑1 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                         >  SRR4302543.618848/1‑94 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                         >  SRR4302543.1973448/1‑94 (MQ=60)

                                                        GTAGAAAAATCGTTATGTCGTAAGGTATGCGCCTCCTCCATACGAGCGACCTGCCAGGTGCTCATGAACGTTTCTGCATAAGGCAGTAGTTTTT                                        <  SRR4302543.7069521/94‑1 (MQ=55)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302543.1394861/1‑94 (MQ=60)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302543.955325/1‑94 (MQ=60)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302543.4002907/1‑94 (MQ=60)

                                                        ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        <  SRR4302543.5331999/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       <  SRR4302543.134510/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       <  SRR4302543.6596631/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                       >  SRR4302543.3059829/1‑94 (MQ=60)

                                                          AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      <  SRR4302543.7798732/94‑1 (MQ=60)

                                                          AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302543.5667558/1‑94 (MQ=60)

                                                          AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302543.4607356/1‑94 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                    <  SRR4302543.2251624/94‑1 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                    >  SRR4302543.5138873/1‑94 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                    >  SRR4302543.1693420/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   <  SRR4302543.5940103/94‑1 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   <  SRR4302543.7879895/94‑1 (MQ=60)

                                                              AACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                  >  SRR4302543.576141/1‑94 (MQ=60)

                                                               ACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 >  SRR4302543.7023089/1‑94 (MQ=60)

                                                                CTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCA                                <  SRR4302543.522171/94‑1 (MQ=60)

                                                                CTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCA                                <  SRR4302543.522172/94‑1 (MQ=60)

                                                                CTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCA                                >  SRR4302543.1442290/1‑94 (MQ=60)

                                                                CTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCA                                >  SRR4302543.1831793/1‑94 (MQ=60)

                                                                  CGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                              <  SRR4302543.6165991/94‑1 (MQ=60)

                                                                  CGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                              <  SRR4302543.3994992/94‑1 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             >  SRR4302543.1953871/1‑94 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302543.7619894/94‑1 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302543.6874076/94‑1 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             >  SRR4302543.7018671/1‑94 (MQ=60)

                                                                   GTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302543.3946812/94‑1 (MQ=60)

                                                                     TATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGT                           >  SRR4302543.3434729/1‑94 (MQ=60)

                                                                     TATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGT                           >  SRR4302543.7265198/1‑94 (MQ=60)

                                                                      ATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          >  SRR4302543.2042646/1‑94 (MQ=60)

                                                                      ATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          >  SRR4302543.6731714/1‑94 (MQ=60)

                                                                      ATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          >  SRR4302543.6731713/1‑94 (MQ=60)

                                                                      ATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302543.6324275/94‑1 (MQ=60)

                                                                      ATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          >  SRR4302543.3676958/1‑94 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                         <  SRR4302543.2879024/94‑1 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                         <  SRR4302543.2983940/94‑1 (MQ=60)

                                                                        GTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAA                        <  SRR4302543.6050615/94‑1 (MQ=60)

                                                                         TCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAA                       <  SRR4302543.2938289/94‑1 (MQ=60)

                                                                         TCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAA                       >  SRR4302543.143118/1‑94 (MQ=60)

                                                                         TCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAA                       <  SRR4302543.2888462/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302543.7806588/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302543.3693155/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302543.6194801/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      <  SRR4302543.4591401/94‑1 (MQ=60)

                                                                           GTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                     <  SRR4302543.7832696/94‑1 (MQ=60)

                                                                           GTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                     >  SRR4302543.3539442/1‑94 (MQ=60)

                                                                           GTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                     >  SRR4302543.4243992/1‑94 (MQ=60)

                                                                           GTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                     <  SRR4302543.5757025/94‑1 (MQ=60)

                                                                            TGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    >  SRR4302543.1619992/1‑94 (MQ=60)

                                                                            TGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    >  SRR4302543.6351471/1‑94 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                   >  SRR4302543.4916651/1‑94 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302543.2542492/94‑1 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  >  SRR4302543.5943175/1‑94 (MQ=60)

                                                                              AGGTATGCCCCACTTCCTGACGGGCGGCCGGCCAGGTGCTCATGCGCGTTTCGGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302543.2542491/94‑1 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCCACGCCAGCGGTTACACGGATT                 >  SRR4302543.7777580/1‑94 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 >  SRR4302543.4279051/1‑94 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                <  SRR4302543.6798815/94‑1 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                >  SRR4302543.5103237/1‑94 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                >  SRR4302543.2360320/1‑94 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                <  SRR4302543.4232525/94‑1 (MQ=60)

                                                                                 TATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATT               >  SRR4302543.7148587/1‑94 (MQ=60)

                                                                                  ATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              >  SRR4302543.5569059/1‑94 (MQ=60)

                                                                                  ATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              >  SRR4302543.3680060/1‑94 (MQ=60)

                                                                                  ATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              <  SRR4302543.3593316/94‑1 (MQ=60)

                                                                                    GCGCCTCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302543.2760128/94‑1 (MQ=60)

                                                                                    GCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302543.2760130/94‑1 (MQ=60)

                                                                                    GCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302543.5888038/94‑1 (MQ=60)

                                                                                     CGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTT           >  SRR4302543.5657121/1‑94 (MQ=60)

                                                                                     CGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTT           <  SRR4302543.4350023/94‑1 (MQ=60)

                                                                                      GCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          >  SRR4302543.66818/1‑94 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302543.5786443/1‑94 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302543.305517/1‑94 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         <  SRR4302543.7871334/94‑1 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302543.3518875/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302543.2148207/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        <  SRR4302543.6527923/94‑1 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302543.7562005/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        <  SRR4302543.6932797/94‑1 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        >  SRR4302543.768157/1‑94 (MQ=60)

                                                                                        CACCTCCTTACAGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG        <  SRR4302543.6932796/94‑1 (MQ=60)

                                                                                         ACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGT       >  SRR4302543.2280238/1‑94 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCGGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG      >  SRR4302543.3279691/1‑94 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     <  SRR4302543.6741796/91‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302543.5839835/4‑94 (MQ=60)

                                                                                            tcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC    <  SRR4302543.5407297/92‑1 (MQ=60)

                                                                                            C‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG   >  SRR4302543.6839519/1‑94 (MQ=60)

                                                                                            C‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG   <  SRR4302543.4220251/94‑1 (MQ=60)

                                                                                              CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  SRR4302543.3050951/1‑94 (MQ=60)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945258‑3945444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`