Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,949,187 A→G Q336R (CAG→CGG)  ilvG → acetolactate synthase II large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,949,1870AG100.0% 711.2 / NA 228Q336R (CAG→CGG) ilvGacetolactate synthase II large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (133/95);  total (133/95)

Only 100 of 243 total aligned reads displayed.

GGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAC  >  NC_000913/3949097‑3949276
                                                                                          |                                                                                         
ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGc                                                                                         >  1:4006301/1‑93 (MQ=255)
ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGc                                                                                         >  1:678604/1‑93 (MQ=255)
ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGc                                                                                         >  2:3847847/1‑93 (MQ=255)
 gCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCt                                                                                        <  2:3401097/93‑1 (MQ=255)
 gCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCt                                                                                        >  1:1811575/1‑93 (MQ=255)
   acaTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGc                                                                                      >  2:2943316/1‑93 (MQ=255)
    caTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCg                                                                                     <  1:57816/93‑1 (MQ=255)
    caTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCg                                                                                     >  1:510242/1‑93 (MQ=255)
     aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGt                                                                                    <  2:1167450/93‑1 (MQ=255)
      tgtgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGtg                                                                                   >  2:3918931/1‑93 (MQ=255)
         ggCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGtgatg                                                                                >  1:2873293/1‑93 (MQ=255)
         ggCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGtgatg                                                                                >  2:1269690/1‑93 (MQ=255)
          gCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTAGCAGCAACACTGCGCGCGGCTGCGtgatga                                                                               >  1:2799746/1‑93 (MQ=255)
           cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGaa                                                                              <  2:4122678/93‑1 (MQ=255)
           cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGaa                                                                              <  1:304739/93‑1 (MQ=255)
            aTTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGaaa                                                                             >  1:3805153/1‑92 (MQ=255)
            aTTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAAc                                                                             <  1:459488/93‑1 (MQ=255)
             ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACa                                                                            <  1:3257589/93‑1 (MQ=255)
             ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACa                                                                            >  1:2668962/1‑93 (MQ=255)
             ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACa                                                                            <  1:4480767/93‑1 (MQ=255)
              tACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAt                                                                           >  1:3489251/1‑93 (MQ=255)
              tACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAt                                                                           >  2:1806102/1‑93 (MQ=255)
              tACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAt                                                                           >  2:1881455/1‑93 (MQ=255)
               aCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAtt                                                                          >  1:1663618/1‑93 (MQ=255)
               aCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAtt                                                                          >  1:193635/1‑93 (MQ=255)
                 taGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTcc                                                                        >  1:4185936/2‑93 (MQ=255)
                 aaGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTcc                                                                        >  1:2516069/1‑93 (MQ=255)
                 aaGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTcc                                                                        <  2:732721/93‑1 (MQ=255)
                      gATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCg                                                                   >  2:4235674/1‑93 (MQ=255)
                      gATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCg                                                                   >  1:3758707/1‑93 (MQ=255)
                         ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTa                                                                >  2:4346291/1‑93 (MQ=255)
                          tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTAc                                                               <  2:4433987/93‑1 (MQ=255)
                              tGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGAcc                                                           >  2:2295960/1‑93 (MQ=255)
                               gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                          >  2:8988/1‑93 (MQ=255)
                               gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                          >  1:2765557/1‑93 (MQ=255)
                               gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGCCTGCGTGATGAACATTCCTGGCTTTACGAGCa                                                          >  1:247800/1‑93 (MQ=255)
                                 tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATc                                                        >  2:1119108/1‑93 (MQ=255)
                                 tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATc                                                        <  1:2851257/93‑1 (MQ=255)
                                 tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATc                                                        >  1:1525474/1‑93 (MQ=255)
                                   tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATccc                                                      >  2:320441/1‑93 (MQ=255)
                                     ttACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCgg                                                    >  1:3515194/1‑93 (MQ=255)
                                         cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAc                                                <  1:1204295/93‑1 (MQ=255)
                                          aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg                                               >  2:1779185/1‑93 (MQ=255)
                                          aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg                                               >  1:2183084/1‑93 (MQ=255)
                                          aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg                                               >  2:1590346/1‑93 (MQ=255)
                                           gCATTACATCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGc                                              >  2:4568786/1‑93 (MQ=255)
                                           gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGc                                              >  2:2545234/1‑93 (MQ=255)
                                           gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGc                                              >  2:3379861/1‑93 (MQ=255)
                                             aTTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTa                                            >  1:489679/1‑93 (MQ=255)
                                             aTTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTa                                            >  2:2633032/1‑93 (MQ=255)
                                               tgcagcagCCGTTAAATCAATGACGGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc                                          <  1:142539/91‑1 (MQ=255)
                                               tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc                                          <  1:602360/93‑1 (MQ=255)
                                               tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc                                          <  2:1427610/93‑1 (MQ=255)
                                                 cagcagCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGAGTGATGAACCTTCCTGGCGTTACGACCATCCCGGTGACGCTATCtc                                        >  2:671708/1‑92 (MQ=255)
                                                  agcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAc                                       <  2:3056897/93‑1 (MQ=255)
                                                   gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg                                      <  2:600763/93‑1 (MQ=255)
                                                   gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg                                      >  2:2521105/1‑93 (MQ=255)
                                                     agcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTAcgcg                                    >  2:1125508/1‑93 (MQ=255)
                                                         gTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgt                                >  1:4057762/1‑93 (MQ=255)
                                                          ttAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt                               >  2:3350721/1‑93 (MQ=255)
                                                          ttAAATCAATGACTGGCAGCAACACTGCGCACGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt                               >  2:3688538/1‑93 (MQ=255)
                                                            aaaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgt                             >  1:2836790/1‑93 (MQ=255)
                                                            aaaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgt                             <  2:1045583/93‑1 (MQ=255)
                                                             aaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt                            >  1:3642137/1‑93 (MQ=255)
                                                             aaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt                            <  1:4269638/93‑1 (MQ=255)
                                                             aaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt                            >  1:2393317/1‑93 (MQ=255)
                                                             aaTCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCCCCgttgtt                            >  1:4036687/1‑93 (MQ=255)
                                                                 aaTGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                        >  2:2269699/1‑93 (MQ=255)
                                                                 aaTGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                        >  1:3960563/1‑93 (MQ=255)
                                                                   tGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                      <  1:4206649/93‑1 (MQ=255)
                                                                    gACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                     >  1:4407508/1‑93 (MQ=255)
                                                                    gACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                     <  2:2059053/93‑1 (MQ=255)
                                                                    gACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                     >  2:4609336/1‑93 (MQ=255)
                                                                    gACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                     >  2:2997947/1‑93 (MQ=255)
                                                                     aCTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                    <  1:2038766/93‑1 (MQ=255)
                                                                       tGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaa                  <  2:2785012/93‑1 (MQ=255)
                                                                        ggCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaac                 >  1:2367308/1‑93 (MQ=255)
                                                                         gcagcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACt                <  1:2134352/93‑1 (MQ=255)
                                                                         gcagcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACt                >  1:361291/1‑93 (MQ=255)
                                                                         gcagcaACACTGCGCGCGGCTGCGTGATGAACATTCCAGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACt                <  1:71214/93‑1 (MQ=255)
                                                                          cagcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTg               <  2:1651576/93‑1 (MQ=255)
                                                                           agcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt              <  1:1387949/93‑1 (MQ=255)
                                                                           agcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt              >  1:2314299/1‑93 (MQ=255)
                                                                           agcaACACTGCGCGCGGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt              <  2:3558454/93‑1 (MQ=255)
                                                                            gcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc             <  2:4661149/93‑1 (MQ=255)
                                                                            gcaACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc             >  1:3869440/1‑93 (MQ=255)
                                                                             gaaCACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg            <  1:3712602/92‑1 (MQ=255)
                                                                             caACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg            <  2:478566/93‑1 (MQ=255)
                                                                              aaCACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg           >  1:1070575/1‑93 (MQ=255)
                                                                              aaCACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg           >  1:96612/1‑93 (MQ=255)
                                                                              aaCACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg           >  2:4001058/1‑93 (MQ=255)
                                                                               acacTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCggt          >  2:2826447/1‑92 (MQ=255)
                                                                                cacTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGAt         <  2:526091/93‑1 (MQ=255)
                                                                                 acTGCGCGCGGCTGCGTGATGAACATTCCTGGCTTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc        <  1:3199530/93‑1 (MQ=255)
                                                                                   tGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt      >  1:4314313/1‑93 (MQ=255)
                                                                                   tGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt      >  2:3489537/1‑93 (MQ=255)
                                                                                      gcgcGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa   >  1:1032686/1‑93 (MQ=255)
                                                                                      gcgcGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa   <  2:2139676/93‑1 (MQ=255)
                                                                                       cgcgGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAc  <  1:773183/93‑1 (MQ=255)
                                                                                       cgcgGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAc  <  2:363016/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAC  >  NC_000913/3949097‑3949276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGC  >  NC_000913/3949094‑3949280
                                                                                             |                                                                                             
TCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCG                                                                                               >  SRR4302535.55627/1‑94 (MQ=60)
 CAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGG                                                                                              >  SRR4302535.1228503/1‑94 (MQ=60)
 CAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGG                                                                                              >  SRR4302535.3772113/1‑94 (MQ=60)
  AGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGC                                                                                             >  SRR4302535.683350/1‑94 (MQ=60)
  AGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGC                                                                                             >  SRR4302535.4033705/1‑94 (MQ=60)
  AGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGC                                                                                             >  SRR4302535.4049823/1‑94 (MQ=60)
   GGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCT                                                                                            >  SRR4302535.1824674/1‑94 (MQ=60)
      ACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCG                                                                                         >  SRR4302535.513811/1‑94 (MQ=60)
       CATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGT                                                                                        <  SRR4302535.58238/94‑1 (MQ=60)
       CATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGT                                                                                        >  SRR4302535.3663262/1‑94 (MQ=60)
        ATGTGGCATTACAAGGTGATTTAAATGCTCTGTTCCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTG                                                                                       <  SRR4302535.4362305/94‑1 (MQ=60)
        ATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTG                                                                                       >  SRR4302535.226283/1‑94 (MQ=60)
        ATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTG                                                                                       >  SRR4302535.526479/1‑94 (MQ=60)
          GTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGAT                                                                                     <  SRR4302535.3788972/94‑1 (MQ=60)
          GTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGAT                                                                                     >  SRR4302535.2767517/1‑94 (MQ=60)
           TGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATG                                                                                    >  SRR4302535.2893651/1‑94 (MQ=60)
           TGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATG                                                                                    <  SRR4302535.2556497/94‑1 (MQ=60)
           TGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATG                                                                                    <  SRR4302535.1580960/94‑1 (MQ=60)
            GGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGA                                                                                   <  SRR4302535.1054392/94‑1 (MQ=60)
            GGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTAGCAGCAACACTGCGCGCGGCTGCGTGATGA                                                                                   >  SRR4302535.2819664/1‑94 (MQ=60)
              CATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAAC                                                                                 >  SRR4302535.4315695/1‑94 (MQ=60)
              CATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAAC                                                                                 <  SRR4302535.306854/94‑1 (MQ=60)
              CATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAAA                                                                                 >  SRR4302535.3831382/1‑94 (MQ=60)
               ATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACA                                                                                >  SRR4302535.41982/1‑94 (MQ=60)
               ATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACA                                                                                <  SRR4302535.462700/94‑1 (MQ=60)
               ATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACA                                                                                >  SRR4302535.2688063/1‑94 (MQ=60)
               ATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACA                                                                                <  SRR4302535.587635/94‑1 (MQ=60)
                TTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAT                                                                               >  SRR4302535.3513496/1‑94 (MQ=60)
                TTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAT                                                                               <  SRR4302535.3280224/94‑1 (MQ=60)
                TTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACAT                                                                               <  SRR4302535.4511523/94‑1 (MQ=60)
                 TACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATT                                                                              >  SRR4302535.1675638/1‑94 (MQ=60)
                 TACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATT                                                                              >  SRR4302535.194971/1‑94 (MQ=60)
                 TACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATT                                                                              >  SRR4302535.734292/1‑94 (MQ=60)
                   CTAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCC                                                                            >  SRR4302535.4214587/1‑94 (MQ=60)
                   CAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCC                                                                            >  SRR4302535.3765578/1‑94 (MQ=60)
                   CAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCC                                                                            >  SRR4302535.2534205/1‑94 (MQ=60)
                     AGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTG                                                                          >  SRR4302535.1403452/1‑94 (MQ=60)
                      GGTGATTTAAATGCTCTGTGTCCAGCACTACAGCAGCCGTTAAATCAATGACTGGCAGCACCACTGCGCGCGGCTGCGTGATGAACATTCCTGG                                                                         <  SRR4302535.4629575/94‑1 (MQ=60)
                       GTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGC                                                                        >  SRR4302535.329420/1‑94 (MQ=60)
                        TGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCG                                                                       >  SRR4302535.3784693/1‑94 (MQ=60)
                          ATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTT                                                                     <  SRR4302535.234497/94‑1 (MQ=60)
                           TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTA                                                                    >  SRR4302535.1168239/1‑94 (MQ=60)
                           TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTA                                                                    >  SRR4302535.1237956/1‑94 (MQ=60)
                            TTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTAC                                                                   >  SRR4302535.3194061/1‑94 (MQ=60)
                            TTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTAC                                                                   <  SRR4302535.2859545/94‑1 (MQ=60)
                             TAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACG                                                                  <  SRR4302535.2088694/94‑1 (MQ=60)
                              AAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGA                                                                 >  SRR4302535.4079537/1‑94 (MQ=60)
                               AATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAATATTCCTGGCGTTACGGC                                                                >  SRR4302535.3225802/1‑94 (MQ=60)
                                agtCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACC                                                               <  SRR4302535.1486606/91‑1 (MQ=60)
                                ATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACC                                                               >  SRR4302535.692642/1‑94 (MQ=60)
                                ATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACC                                                               <  SRR4302535.3613978/94‑1 (MQ=60)
                                 TGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCA                                                              >  SRR4302535.2785253/1‑94 (MQ=60)
                                 TGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGCCTGCGTGATGAACATTCCTGGCTTTACGAGCA                                                              >  SRR4302535.249502/1‑94 (MQ=60)
                                  GCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCAT                                                             <  SRR4302535.3547209/94‑1 (MQ=60)
                                   CTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATC                                                            >  SRR4302535.1536464/1‑94 (MQ=60)
                                    TCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCC                                                           <  SRR4302535.2871462/94‑1 (MQ=60)
                                    TCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCC                                                           <  SRR4302535.4287389/94‑1 (MQ=60)
                                     CTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCC                                                          <  SRR4302535.2788505/94‑1 (MQ=60)
                                     CTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCC                                                          <  SRR4302535.2386857/94‑1 (MQ=60)
                                       GTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGG                                                        >  SRR4302535.3539620/1‑94 (MQ=60)
                                       GTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGG                                                        <  SRR4302535.3876553/94‑1 (MQ=60)
                                       GTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGG                                                        >  SRR4302535.2416539/1‑94 (MQ=60)
                                         TACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTG                                                      >  SRR4302535.397877/1‑94 (MQ=60)
                                          ACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGA                                                     >  SRR4302535.1808612/1‑94 (MQ=60)
                                           CCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAC                                                    >  SRR4302535.3154098/1‑94 (MQ=60)
                                           CCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCCGGCGTTACGACCATCCCGGTGAC                                                    <  SRR4302535.2083006/94‑1 (MQ=60)
                                            CAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACG                                                   <  SRR4302535.1212940/94‑1 (MQ=60)
                                            CAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACG                                                   <  SRR4302535.625635/94‑1 (MQ=60)
                                            CAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACG                                                   >  SRR4302535.2198651/1‑94 (MQ=60)
                                            CAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACG                                                   >  SRR4302535.1782673/1‑94 (MQ=60)
                                               CATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTA                                                >  SRR4302535.3993313/1‑94 (MQ=60)
                                               CATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTA                                                >  SRR4302535.2562391/1‑94 (MQ=60)
                                               CATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTA                                                >  SRR4302535.493117/1‑94 (MQ=60)
                                               CATTACAGCAGCCGTTAAATCAATGACTCGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTA                                                >  SRR4302535.2837801/1‑94 (MQ=60)
                                                  TGCAGCAGCCGTTAAATCAATGACGGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCT                                             <  SRR4302535.143535/94‑1 (MQ=60)
                                                  TACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCT                                             <  SRR4302535.606576/94‑1 (MQ=60)
                                                   ACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA                                            >  SRR4302535.2861881/1‑94 (MQ=60)
                                                   ACAGCAGCCGTTAAATCAATGACTGGCAGCAACACGGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA                                            <  SRR4302535.399069/94‑1 (MQ=60)
                                                    CAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAC                                           <  SRR4302535.151665/94‑1 (MQ=60)
                                                     AGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACG                                          >  SRR4302535.499656/1‑94 (MQ=60)
                                                     AGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACG                                          <  SRR4302535.4234530/94‑1 (MQ=60)
                                                     AGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACG                                          <  SRR4302535.4355009/94‑1 (MQ=60)
                                                      GCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGC                                         <  SRR4302535.842925/94‑1 (MQ=60)
                                                       CAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCG                                        <  SRR4302535.3859995/94‑1 (MQ=60)
                                                          CCGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCG                                     >  SRR4302535.533710/1‑94 (MQ=60)
                                                           CGTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGT                                    >  SRR4302535.4085538/1‑94 (MQ=60)
                                                            GTTAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTT                                   <  SRR4302535.1923211/94‑1 (MQ=60)
                                                              TAAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGT                                 >  SRR4302535.2856923/1‑94 (MQ=60)
                                                               AAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTT                                >  SRR4302535.2410518/1‑94 (MQ=60)
                                                               AAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTT                                >  SRR4302535.3667362/1‑94 (MQ=60)
                                                               AAATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCCCCGTTGTT                                >  SRR4302535.4064318/1‑94 (MQ=60)
                                                                AATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTG                               <  SRR4302535.4298870/94‑1 (MQ=60)
                                                                AATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTG                               >  SRR4302535.3354387/1‑94 (MQ=60)
                                                                 ATCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGT                              >  SRR4302535.865522/1‑94 (MQ=60)
                                                                  TCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTT                             <  SRR4302535.4242523/94‑1 (MQ=60)
                                                                  TCAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTT                             <  SRR4302535.4693760/94‑1 (MQ=60)
                                                                   CAATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTA                            >  SRR4302535.3987611/1‑94 (MQ=60)
                                                                    AATGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA                           >  SRR4302535.2803592/1‑94 (MQ=60)
                                                                      TGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAA                         <  SRR4302535.4235436/94‑1 (MQ=60)
                                                                      TGACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAA                         >  SRR4302535.4437670/1‑94 (MQ=60)
                                                                       GACTGGCAGCAACGCTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAC                        <  SRR4302535.2882168/94‑1 (MQ=60)
                                                                        ACTGGCAGCAACACTGCGCGCGGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACA                       <  SRR4302535.629451/94‑1 (MQ=60)
                                                                        ACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACA                       <  SRR4302535.2918999/94‑1 (MQ=60)
                                                                        ACTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACA                       <  SRR4302535.2053446/94‑1 (MQ=60)
                                                                         CTGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAA                      <  SRR4302535.3539283/94‑1 (MQ=60)
                                                                          TGGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAAC                     >  SRR4302535.2384305/1‑94 (MQ=60)
                                                                           GGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACT                    >  SRR4302535.363786/1‑94 (MQ=60)
                                                                           GGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACT                    <  SRR4302535.2388781/94‑1 (MQ=60)
                                                                           GGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACT                    <  SRR4302535.4601307/94‑1 (MQ=60)
                                                                           GGCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACT                    <  SRR4302535.4208939/94‑1 (MQ=60)
                                                                            GCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTG                   <  SRR4302535.2149732/94‑1 (MQ=60)
                                                                            GCAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCAGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTG                   <  SRR4302535.71736/94‑1 (MQ=60)
                                                                             CAGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGT                  >  SRR4302535.2330857/1‑94 (MQ=60)
                                                                              AGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTC                 <  SRR4302535.1397853/94‑1 (MQ=60)
                                                                              AGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTC                 >  SRR4302535.3717939/1‑94 (MQ=60)
                                                                              AGCAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTC                 >  SRR4302535.3896035/1‑94 (MQ=60)
                                                                                GAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGG               <  SRR4302535.3738321/94‑1 (MQ=60)
                                                                                GAACACTGCACGCGGCTGCGTGATGACCATTCCTGGCGTTACGTCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGG               <  SRR4302535.368752/94‑1 (MQ=60)
                                                                                CAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGG               >  SRR4302535.97295/1‑94 (MQ=60)
                                                                                CAACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGG               >  SRR4302535.1078187/1‑94 (MQ=60)
                                                                                 AACACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGA              >  SRR4302535.2954638/1‑94 (MQ=60)
                                                                                   CACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATC            <  SRR4302535.2267890/94‑1 (MQ=60)
                                                                                   CACTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATC            >  SRR4302535.3760079/1‑94 (MQ=60)
                                                                                   CACTGCGCCGGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATC            <  SRR4302535.4510790/94‑1 (MQ=60)
                                                                                    ACTGCGCGCGGCTGCGTGATGAACATTCCTGGCTTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCG           <  SRR4302535.3221680/94‑1 (MQ=60)
                                                                                     CTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGT          >  SRR4302535.2504566/1‑94 (MQ=60)
                                                                                     CTGCGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGT          >  SRR4302535.4343826/1‑94 (MQ=60)
                                                                                        CGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAA       >  SRR4302535.1040022/1‑94 (MQ=60)
                                                                                        CGCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAA       <  SRR4302535.1974920/94‑1 (MQ=60)
                                                                                         GCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAC      >  SRR4302535.1240090/1‑94 (MQ=60)
                                                                                         GCGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAC      <  SRR4302535.215554/94‑1 (MQ=60)
                                                                                          CGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACC     <  SRR4302535.485943/94‑1 (MQ=60)
                                                                                          CGCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACC     <  SRR4302535.778621/94‑1 (MQ=60)
                                                                                           GCGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCT    <  SRR4302535.96396/94‑1 (MQ=60)
                                                                                            CGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTG   <  SRR4302535.2234495/94‑1 (MQ=60)
                                                                                            CGGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTG   <  SRR4302535.2224137/94‑1 (MQ=60)
                                                                                             GGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGC  >  SRR4302535.722745/1‑94 (MQ=60)
                                                                                             GGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGC  >  SRR4302535.1318227/1‑94 (MQ=60)
                                                                                             GGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAACTGC  >  SRR4302535.1750034/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGC  >  NC_000913/3949094‑3949280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑