Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,953,910	A→G	N320S (AAC→AGC)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,953,910	0	A	G	100.0%	612.8 / NA	198	N320S (AAC→AGC)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (90/108); total (90/108)

Only 100 of 208 total aligned reads displayed.

CGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAG  >  NC_000913/3953820‑3953999

cgctggcgctggCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCt                                                                                         <  2:2606967/93‑1 (MQ=255)

cgctggcgctggCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCt                                                                                         <  2:4506607/93‑1 (MQ=255)

  ctggcgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTc                                                                                       >  1:4284179/1‑93 (MQ=255)

  ctggcgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTc                                                                                       >  2:3813340/1‑93 (MQ=255)

   tggcgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTcc                                                                                      >  1:492358/1‑93 (MQ=255)

    ggcgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCa                                                                                     <  1:1249547/93‑1 (MQ=255)

    ggcgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCa                                                                                     <  1:2113320/93‑1 (MQ=255)

      cgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACg                                                                                   <  1:1436747/93‑1 (MQ=255)

      cgctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACg                                                                                   <  2:4305661/93‑1 (MQ=255)

       gctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACgg                                                                                  >  1:680266/1‑93 (MQ=255)

       gctggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACgg                                                                                  <  1:4434727/93‑1 (MQ=255)

          ggcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCt                                                                               <  2:2013389/93‑1 (MQ=255)

           gcgGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCCGAGCCAACGTGAGCTTCCACGGCCTg                                                                              >  1:3800331/1‑93 (MQ=255)

             gggAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTgcg                                                                            >  1:1007784/1‑93 (MQ=255)

             gggAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCCTTCCGGTGGCAAAGTGAGCTTTCAAGTCCTccg                                                                            >  2:2849156/1‑93 (MQ=255)

              ggAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTgcgc                                                                           <  1:3794620/93‑1 (MQ=255)

              ggAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTgcgc                                                                           >  2:2801803/1‑93 (MQ=255)

              ggAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCGTCCACGGTCTgcgc                                                                           >  1:2205681/1‑93 (MQ=255)

               gAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCt                                                                          <  1:4391538/93‑1 (MQ=255)

                 aTGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGGCAACGTGAGCTTCCACGGCCTGCGCTAc                                                                        >  1:1946545/1‑93 (MQ=255)

                  tGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACg                                                                       >  2:830813/1‑93 (MQ=255)

                   gAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGt                                                                      >  1:214645/1‑93 (MQ=255)

                     aaaaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGGCTGCGCTACGtct                                                                    >  2:2383696/1‑93 (MQ=255)

                     aaaaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGtct                                                                    <  2:3539509/93‑1 (MQ=255)

                     aaaaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGtct                                                                    >  1:2002726/1‑93 (MQ=255)

                     aaaaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGtct                                                                    >  2:1834834/1‑93 (MQ=255)

                      aaaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGtctc                                                                   <  1:3845994/93‑1 (MQ=255)

                       aaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCa                                                                  >  2:186370/1‑93 (MQ=255)

                       aaaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCa                                                                  >  2:3745657/1‑93 (MQ=255)

                        aaTATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACCGCCTGCGCTACGTCTCAg                                                                 >  2:900936/1‑93 (MQ=255)

                         atatatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGa                                                                >  2:3405147/1‑93 (MQ=255)

                          tatatCGCCCTGCACAACATTCGCGGCGAACGGCTGGGGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGaa                                                               >  1:1318621/1‑93 (MQ=255)

                          tatatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGaa                                                               <  1:3400718/93‑1 (MQ=255)

                          tatatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGaa                                                               <  2:2484379/93‑1 (MQ=255)

                          tatatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGaa                                                               <  2:4200288/93‑1 (MQ=255)

                           atatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAAc                                                              >  1:3374395/1‑93 (MQ=255)

                            tatCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACg                                                             >  1:1742724/1‑93 (MQ=255)

                             atCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGc                                                            <  1:1876634/93‑1 (MQ=255)

                             atCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGc                                                            <  1:1457351/93‑1 (MQ=255)

                              tCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCt                                                           <  2:3100824/93‑1 (MQ=255)

                               cGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTg                                                          <  2:4408910/93‑1 (MQ=255)

                                gCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGc                                                         <  2:4038434/93‑1 (MQ=255)

                                  ccTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGa                                                       >  1:1607494/1‑93 (MQ=255)

                                  ccTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAAAGCTGCGa                                                       >  2:974821/1‑93 (MQ=255)

                                     gCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACt                                                    >  2:2826563/1‑93 (MQ=255)

                                       acaacaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTgg                                                  >  1:3537679/1‑93 (MQ=255)

                                       acaacaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTgg                                                  >  1:973949/1‑93 (MQ=255)

                                        caacaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTggg                                                 <  1:1305860/93‑1 (MQ=255)

                                        caacaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTggg                                                 >  2:757535/1‑93 (MQ=255)

                                         aacaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGc                                                >  1:1085284/1‑93 (MQ=255)

                                          acaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCg                                               >  1:3097163/1‑93 (MQ=255)

                                          acaTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCg                                               >  2:281066/1‑93 (MQ=255)

                                            aTTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGaa                                             <  1:4585199/93‑1 (MQ=255)

                                             ttCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGaaa                                            >  1:3704916/1‑92 (MQ=255)

                                             ttCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAc                                            <  1:3173976/93‑1 (MQ=255)

                                             ttCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAc                                            >  2:4362064/1‑93 (MQ=255)

                                             ttCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAc                                            >  1:3881147/1‑93 (MQ=255)

                                             ttCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAc                                            >  1:4018697/1‑93 (MQ=255)

                                               cgcgGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAg                                          <  2:2088165/93‑1 (MQ=255)

                                                gcggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGc                                         <  1:3025076/93‑1 (MQ=255)

                                                gcggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGc                                         <  2:2237279/93‑1 (MQ=255)

                                                 cggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCg                                        <  2:2972921/93‑1 (MQ=255)

                                                 cggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCg                                        <  1:3335199/93‑1 (MQ=255)

                                                  ggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGt                                       <  2:4627433/93‑1 (MQ=255)

                                                  ggcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGt                                       >  1:4569217/1‑93 (MQ=255)

                                                   gcgAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTg                                      >  2:3309317/1‑93 (MQ=255)

                                                     gAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGaa                                    >  1:3724290/1‑93 (MQ=255)

                                                      aaCGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAg                                   >  1:1844193/1‑93 (MQ=255)

                                                        cGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCg                                 <  1:1241740/93‑1 (MQ=255)

                                                         ggCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCgt                                <  1:1754200/93‑1 (MQ=255)

                                                         ggCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCgt                                >  1:4053233/1‑93 (MQ=255)

                                                             ggCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCgttgtt                            <  1:1635283/93‑1 (MQ=255)

                                                             ggCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCgttgtt                            <  2:1406689/93‑1 (MQ=255)

                                                               cgcATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTgg                          <  1:4089067/93‑1 (MQ=255)

                                                               cgcATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTgg                          <  1:2978888/93‑1 (MQ=255)

                                                                gcATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGc                         <  1:3465677/93‑1 (MQ=255)

                                                                 cATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGGTGTTGGCg                        >  2:299027/1‑93 (MQ=255)

                                                                  atatTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCgg                       <  2:1625318/93‑1 (MQ=255)

                                                                  atatTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCgg                       >  2:1103040/1‑93 (MQ=255)

                                                                   tatTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGt                      <  1:784928/93‑1 (MQ=255)

                                                                   tatTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGCTGTTGGCggg                      >  1:626346/1‑92 (MQ=255)

                                                                     ttCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGa                    <  2:532226/93‑1 (MQ=255)

                                                                     ttCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGa                    >  2:4074447/1‑93 (MQ=255)

                                                                      tCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTATTGGCGGTGAc                   <  1:1903544/93‑1 (MQ=255)

                                                                      tCTTCCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGAc                   <  2:3241884/93‑1 (MQ=255)

                                                                       cTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGAcc                  <  2:1691433/93‑1 (MQ=255)

                                                                       cTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGAcc                  >  1:2401571/1‑93 (MQ=255)

                                                                        tttCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCa                 <  2:3585410/93‑1 (MQ=255)

                                                                         ttCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCAt                <  2:2738899/93‑1 (MQ=255)

                                                                           ccGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTc              <  1:1500576/93‑1 (MQ=255)

                                                                            cGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTcc             <  1:4463518/93‑1 (MQ=255)

                                                                                gCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGgaa         <  2:1264283/93‑1 (MQ=255)

                                                                                   aaCGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGgaagaa      >  2:3620386/1‑93 (MQ=255)

                                                                                    aCGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaa     >  1:2760158/1‑93 (MQ=255)

                                                                                     cGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaaa    >  2:4652835/1‑93 (MQ=255)

                                                                                     cGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaaa    >  2:3181522/1‑93 (MQ=255)

                                                                                      gTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaaaa   <  1:2616171/93‑1 (MQ=255)

                                                                                      gTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaaaa   >  2:3997598/1‑93 (MQ=255)

                                                                                      gTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGaaaaa   >  1:877736/1‑93 (MQ=255)

                                                                                       tGAGCTTCCACGGTCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAg  <  1:2677560/93‑1 (MQ=255)

CGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAG  >  NC_000913/3953820‑3953999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAG  >  NC_000913/3953817‑3954003

GCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAG                                                                                               <  SRR4302535.1480101/94‑1 (MQ=60)

  GCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCT                                                                                             >  SRR4302535.1833090/1‑94 (MQ=60)

    GCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTC                                                                                           >  SRR4302535.4313488/1‑94 (MQ=60)

     CTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCC                                                                                          >  SRR4302535.495810/1‑94 (MQ=60)

       GGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCAC                                                                                        <  SRR4302535.2582530/94‑1 (MQ=60)

       GGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCAC                                                                                        <  SRR4302535.2128573/94‑1 (MQ=60)

       GGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCAC                                                                                        <  SRR4302535.3732946/94‑1 (MQ=60)

       GGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCAC                                                                                        <  SRR4302535.1258467/94‑1 (MQ=60)

         CGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCTGTGCCAACGTGCGCTTCCACCG                                                                                      >  SRR4302535.2158261/1‑94 (MQ=60)

         CGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGG                                                                                      <  SRR4302535.3705844/94‑1 (MQ=60)

         CGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGG                                                                                      <  SRR4302535.1447013/94‑1 (MQ=60)

         CGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGG                                                                                      >  SRR4302535.685032/1‑94 (MQ=60)

          GCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGC                                                                                     <  SRR4302535.4465118/94‑1 (MQ=60)

            TGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCT                                                                                   <  SRR4302535.344750/94‑1 (MQ=60)

             GGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTG                                                                                  <  SRR4302535.4435884/94‑1 (MQ=60)

             GGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTG                                                                                  <  SRR4302535.68522/94‑1 (MQ=60)

             GGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCCGAGCCAACGTGAGCTTCCACGGCCTG                                                                                  >  SRR4302535.3826528/1‑94 (MQ=60)

               CGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCG                                                                                >  SRR4302535.1014945/1‑94 (MQ=60)

                GGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCGTCCACGGTCTGCGC                                                                               >  SRR4302535.2221390/1‑94 (MQ=60)

                 GGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCT                                                                              <  SRR4302535.3820778/94‑1 (MQ=60)

                 GGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCT                                                                              >  SRR4302535.1811014/1‑94 (MQ=60)

                  GAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTA                                                                             <  SRR4302535.4421584/94‑1 (MQ=60)

                   AATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGGCAACGTGAGCTTCCACGGCCTGCGCTAC                                                                            >  SRR4302535.1960556/1‑94 (MQ=60)

                     TGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGT                                                                          >  SRR4302535.3824754/1‑94 (MQ=60)

                     TGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGT                                                                          >  SRR4302535.1481517/1‑94 (MQ=60)

                     TGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGT                                                                          >  SRR4302535.216116/1‑94 (MQ=60)

                       AAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCT                                                                        >  SRR4302535.4109645/1‑94 (MQ=60)

                       AAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCT                                                                        >  SRR4302535.2978758/1‑94 (MQ=60)

                       AAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCT                                                                        >  SRR4302535.2017157/1‑94 (MQ=60)

                       AAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCT                                                                        <  SRR4302535.1920201/94‑1 (MQ=60)

                        AAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTC                                                                       <  SRR4302535.1897234/94‑1 (MQ=60)

                         AAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCA                                                                      <  SRR4302535.3872474/94‑1 (MQ=60)

                            ATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGGGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAA                                                                   >  SRR4302535.1328046/1‑94 (MQ=60)

                            ATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAA                                                                   >  SRR4302535.3841568/1‑94 (MQ=60)

                             TATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAAC                                                                  >  SRR4302535.3397865/1‑94 (MQ=60)

                             TATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAAC                                                                  <  SRR4302535.3424388/94‑1 (MQ=60)

                              ATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACG                                                                 >  SRR4302535.1755326/1‑94 (MQ=60)

                                ATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCT                                                               <  SRR4302535.1313677/94‑1 (MQ=60)

                                ATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCT                                                               <  SRR4302535.1890148/94‑1 (MQ=60)

                                ATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCT                                                               <  SRR4302535.1467777/94‑1 (MQ=60)

                                 TCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTG                                                              <  SRR4302535.2195317/94‑1 (MQ=60)

                                 TCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTG                                                              <  SRR4302535.2241691/94‑1 (MQ=60)

                                    CCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGA                                                           >  SRR4302535.1619090/1‑94 (MQ=60)

                                     CCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAA                                                          >  SRR4302535.1766861/1‑94 (MQ=60)

                                      CTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAAC                                                         >  SRR4302535.1257864/1‑94 (MQ=60)

                                        GCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTG                                                       <  SRR4302535.203148/94‑1 (MQ=60)

                                         CACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGG                                                      <  SRR4302535.2950745/94‑1 (MQ=60)

                                         CACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGG                                                      >  SRR4302535.3562239/1‑94 (MQ=60)

                                         CACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGG                                                      >  SRR4302535.980861/1‑94 (MQ=60)

                                         CACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGG                                                      >  SRR4302535.1880692/1‑94 (MQ=60)

                                          ACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGG                                                     <  SRR4302535.4481712/94‑1 (MQ=60)

                                           CAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGC                                                    >  SRR4302535.1092998/1‑94 (MQ=60)

                                           CAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGC                                                    <  SRR4302535.1315181/94‑1 (MQ=60)

                                            AACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCG                                                   >  SRR4302535.3864912/1‑94 (MQ=60)

                                            AACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCG                                                   >  SRR4302535.3118729/1‑94 (MQ=60)

                                            AACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCG                                                   <  SRR4302535.1631454/94‑1 (MQ=60)

                                              CATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGGCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGGACTGGGCGGA                                                 >  SRR4302535.892283/1‑94 (MQ=60)

                                               ATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAC                                                >  SRR4302535.3907783/1‑94 (MQ=60)

                                               ATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAC                                                <  SRR4302535.4616423/94‑1 (MQ=60)

                                               ATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAC                                                >  SRR4302535.4046193/1‑94 (MQ=60)

                                               ATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAAA                                                >  SRR4302535.3730575/1‑94 (MQ=60)

                                               ACTCGCGGCGAACGGCTGGCGCACATTCGTTCCGGGGCCAACGCGAGCTGCCACGGCCTGCGCTACGCTGCAGTACGCTGCGAACCGGGCGAAC                                                >  SRR4302535.4718663/1‑94 (MQ=28)

                                                TTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACA                                               <  SRR4302535.3195944/94‑1 (MQ=60)

                                                 TCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAG                                              >  SRR4302535.83973/1‑94 (MQ=60)

                                                   GCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCG                                            <  SRR4302535.3046253/94‑1 (MQ=60)

                                                   GCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCG                                            <  SRR4302535.1525357/94‑1 (MQ=60)

                                                   GCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCG                                            >  SRR4302535.3356286/1‑94 (MQ=60)

                                                    CGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGT                                           >  SRR4302535.4600377/1‑94 (MQ=60)

                                                    CGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGT                                           <  SRR4302535.3358425/94‑1 (MQ=60)

                                                     GGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTG                                          <  SRR4302535.3797277/94‑1 (MQ=60)

                                                      GCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGA                                         >  SRR4302535.1407949/1‑94 (MQ=60)

                                                       CGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAA                                        >  SRR4302535.3750091/1‑94 (MQ=60)

                                                       CGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAA                                        <  SRR4302535.4515854/94‑1 (MQ=60)

                                                        GAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAG                                       >  SRR4302535.1703768/1‑94 (MQ=60)

                                                        GAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAG                                       >  SRR4302535.1857491/1‑94 (MQ=60)

                                                         AACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGC                                      <  SRR4302535.4606700/94‑1 (MQ=60)

                                                           CGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGT                                    <  SRR4302535.1250610/94‑1 (MQ=60)

                                                           CGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGT                                    >  SRR4302535.4080987/1‑94 (MQ=60)

                                                            GGCTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTT                                   <  SRR4302535.1766876/94‑1 (MQ=60)

                                                              CTGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGT                                 >  SRR4302535.2730645/1‑94 (MQ=60)

                                                               TGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTT                                <  SRR4302535.353773/94‑1 (MQ=60)

                                                               TGGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTT                                <  SRR4302535.1131219/94‑1 (MQ=60)

                                                                GGCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTG                               <  SRR4302535.1647099/94‑1 (MQ=60)

                                                                 GCGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGG                              >  SRR4302535.3188110/1‑94 (MQ=60)

                                                                  CGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGC                             >  SRR4302535.552450/1‑94 (MQ=60)

                                                                  CGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGC                             <  SRR4302535.4117046/94‑1 (MQ=60)

                                                                  CGCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGC                             <  SRR4302535.2999788/94‑1 (MQ=60)

                                                                   GCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCG                            <  SRR4302535.3113631/94‑1 (MQ=60)

                                                                   GCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCG                            <  SRR4302535.3489761/94‑1 (MQ=60)

                                                                   GCATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCG                            <  SRR4302535.3932560/94‑1 (MQ=60)

                                                                     ATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGT                          >  SRR4302535.2003299/1‑94 (MQ=60)

                                                                     ATATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGCTGTTGGCGGG                          >  SRR4302535.630703/1‑94 (MQ=60)

                                                                      TATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTG                         <  SRR4302535.4055547/94‑1 (MQ=60)

                                                                      TATTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTG                         <  SRR4302535.790434/94‑1 (MQ=60)

                                                                        TTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGAC                       <  SRR4302535.789651/94‑1 (MQ=60)

                                                                        TTCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGAC                       <  SRR4302535.2691213/94‑1 (MQ=60)

                                                                         TCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACC                      >  SRR4302535.2418846/1‑94 (MQ=60)

                                                                         TCTTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTATTGGCGGTGACC                      <  SRR4302535.1917243/94‑1 (MQ=60)

                                                                           TTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCAT                    >  SRR4302535.3874372/1‑94 (MQ=60)

                                                                           CTTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCAT                    <  SRR4302535.1319948/94‑1 (MQ=60)

                                                                            TTCCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATT                   >  SRR4302535.1451980/1‑94 (MQ=60)

                                                                              CCGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCC                 <  SRR4302535.1511370/94‑1 (MQ=60)

                                                                               CGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCG                <  SRR4302535.3364610/94‑1 (MQ=60)

                                                                               CGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCG                <  SRR4302535.4160474/94‑1 (MQ=60)

                                                                               CGGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCG                <  SRR4302535.4494132/94‑1 (MQ=60)

                                                                                GGTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGG               <  SRR4302535.3024141/94‑1 (MQ=60)

                                                                                 GTGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGA              <  SRR4302535.1090463/94‑1 (MQ=60)

                                                                                  TGCCAACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAA             >  SRR4302535.3400942/1‑94 (MQ=60)

                                                                                      AACGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAA         >  SRR4302535.2779827/1‑94 (MQ=60)

                                                                                        CGTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAA       >  SRR4302535.883950/1‑94 (MQ=60)

                                                                                         GTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAG      <  SRR4302535.577846/94‑1 (MQ=60)

                                                                                         GTGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAG      <  SRR4302535.2634930/94‑1 (MQ=60)

                                                                                          TGAGCTTCCACGGTCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGG     <  SRR4302535.2696714/94‑1 (MQ=60)

                                                                                          TGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGG     <  SRR4302535.4694422/94‑1 (MQ=60)

                                                                                          TGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGG     >  SRR4302535.1790210/1‑94 (MQ=60)

                                                                                          TGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGG     <  SRR4302535.4325534/94‑1 (MQ=60)

                                                                                          TGAGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGG     >  SRR4302535.1211354/1‑94 (MQ=60)

                                                                                            AGCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCA   >  SRR4302535.455353/1‑94 (MQ=60)

                                                                                             GCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAG  >  SRR4302535.2372597/1‑94 (MQ=60)

                                                                                             GCTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAG  <  SRR4302535.1405917/94‑1 (MQ=60)

GCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGGCCTGCGCTACGTCTCAGAACGCTGCGAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAG  >  NC_000913/3953817‑3954003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`