Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,588	C→G	R86T (AGG→ACG)	gppA ←	guanosine pentaphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,588	0	C	G	100.0%	933.5 / NA	295	R86T (AGG→ACG)	gppA	guanosine pentaphosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (152/143); total (152/143)

Only 100 of 302 total aligned reads displayed.

TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT  >  NC_000913/3961498‑3961677

tCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTc                                                                                         >  1:1812877/1‑93 (MQ=255)

 ccTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCg                                                                                        >  1:4244790/1‑93 (MQ=255)

  ctctTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGc                                                                                       >  1:2027271/1‑93 (MQ=255)

   tctTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGcc                                                                                      >  1:1177774/1‑93 (MQ=255)

    ctTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCg                                                                                     >  2:2468618/1‑93 (MQ=255)

      tCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTa                                                                                   <  2:4477390/93‑1 (MQ=255)

        aCCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGc                                                                                 <  1:476619/93‑1 (MQ=255)

         ccGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCg                                                                                <  2:2027449/93‑1 (MQ=255)

           gCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAc                                                                              >  1:3383365/1‑93 (MQ=255)

            cTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACa                                                                             >  2:4222405/1‑93 (MQ=255)

            cTGATCACCTGTACCGGAAAACCGAGATTTTCCTGCGCTTTGTCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACa                                                                             <  2:4340561/93‑1 (MQ=255)

             tGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa                                                                            >  2:1351115/1‑93 (MQ=255)

             tGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa                                                                            >  2:4491651/1‑93 (MQ=255)

              gATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAc                                                                           >  2:3303464/1‑93 (MQ=255)

               aTCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAca                                                                          <  2:1023915/93‑2 (MQ=255)

               aTCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAca                                                                          <  1:3893375/93‑2 (MQ=255)

                 cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcacg                                                                        >  2:1858308/1‑93 (MQ=255)

                 cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcacg                                                                        <  1:4002368/93‑1 (MQ=255)

                   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGaa                                                                      <  2:4021682/93‑1 (MQ=255)

                   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                      <  2:2753296/93‑1 (MQ=255)

                   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                      >  2:4148505/1‑93 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAGAACACGAAt                                                                     >  2:4080499/1‑93 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAt                                                                     >  1:929288/1‑93 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAt                                                                     <  1:3288392/93‑1 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAt                                                                     <  1:3644335/93‑1 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                    <  1:4285967/93‑1 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                    <  2:1676372/93‑1 (MQ=255)

                      gTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAttt                                                                   <  1:3219395/93‑1 (MQ=255)

                      gTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAttt                                                                   <  1:3687416/93‑1 (MQ=255)

                       tACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTg                                                                  >  2:1651950/1‑93 (MQ=255)

                        aCCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGc                                                                 <  1:1222698/93‑1 (MQ=255)

                          cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCga                                                               <  2:1513018/93‑1 (MQ=255)

                           ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                              <  2:2165732/93‑1 (MQ=255)

                           ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                              >  2:3180104/1‑93 (MQ=255)

                           ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                              <  2:3395857/93‑1 (MQ=255)

                            gACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagg                                                             <  2:510222/93‑1 (MQ=255)

                             aCAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgaggg                                                            <  1:1060365/93‑1 (MQ=255)

                              cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgaggga                                                           <  1:1128246/93‑1 (MQ=255)

                               aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggag                                                          >  2:4677740/1‑93 (MQ=255)

                               aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggag                                                          >  1:2868937/1‑93 (MQ=255)

                                aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggagg                                                         <  2:644745/93‑1 (MQ=255)

                                 ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGggagga                                                        >  1:4445858/1‑93 (MQ=255)

                                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                       >  1:1669117/1‑93 (MQ=255)

                                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                       >  2:2694914/1‑93 (MQ=255)

                                   gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                      >  2:2644161/1‑93 (MQ=255)

                                    agaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAata                                                     <  1:1361964/93‑1 (MQ=255)

                                     gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAatat                                                    >  2:701246/1‑93 (MQ=255)

                                     gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAatat                                                    <  1:1263596/93‑1 (MQ=255)

                                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                   <  2:3733573/93‑1 (MQ=255)

                                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                   <  2:2359132/93‑1 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                  >  1:1538491/1‑93 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                  <  1:461728/93‑1 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                  <  1:4396737/93‑1 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                  >  2:180407/1‑93 (MQ=255)

                                          tCCTGCGCTTTGGCAATAAAATCACCCGCATTGGCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGc                                               <  1:881149/93‑1 (MQ=255)

                                          tCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGc                                               <  2:3952693/93‑1 (MQ=255)

                                            cTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAg                                             <  1:2203708/93‑1 (MQ=255)

                                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGa                                            <  1:3488082/93‑1 (MQ=255)

                                              gcgcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAc                                           <  1:3550232/93‑1 (MQ=255)

                                              gcgcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAc                                           >  2:1199209/1‑93 (MQ=255)

                                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                         <  1:3431526/93‑1 (MQ=255)

                                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                         >  1:4520352/1‑93 (MQ=255)

                                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                         >  1:4652938/1‑93 (MQ=255)

                                                 cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGtt                                        >  2:921357/1‑93 (MQ=255)

                                                      gCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCa                                   <  2:2137041/93‑1 (MQ=255)

                                                        aaTAAAATCACCCGCATTGACGGGAAGGCGTAACGGCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                 >  2:2566213/1‑93 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGGCAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                >  2:3712592/1‑93 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACCACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                >  2:3093272/1‑93 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                <  1:3089830/93‑1 (MQ=255)

                                                          tAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                               <  1:525414/93‑1 (MQ=255)

                                                           aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg                              <  2:3872924/93‑1 (MQ=255)

                                                           aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg                              >  2:477009/1‑93 (MQ=255)

                                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                            <  1:2480859/93‑1 (MQ=255)

                                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                            <  1:2932826/93‑1 (MQ=255)

                                                              aTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcg                           >  1:1003650/1‑93 (MQ=255)

                                                               tCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcgc                          >  2:2878037/1‑93 (MQ=255)

                                                                cACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCa                         >  1:4482616/1‑93 (MQ=255)

                                                                  cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                       >  1:1417301/1‑93 (MQ=255)

                                                                  cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                       >  2:344115/1‑93 (MQ=255)

                                                                    cGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACa                     <  1:4092441/93‑1 (MQ=255)

                                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCACACAt                    >  2:1108844/1‑93 (MQ=255)

                                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt                    <  2:4623188/93‑1 (MQ=255)

                                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt                    <  1:3773503/93‑1 (MQ=255)

                                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGCTCAGAAAACCGGCGCAAACAt                    >  1:792269/1‑93 (MQ=255)

                                                                      cATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAtt                   <  2:3808823/93‑1 (MQ=255)

                                                                       actGACGGCAAGGCGTAACGTCGCCTTAGCGACAACACTAATTTGCGAGTGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                  <  1:1741325/91‑1 (MQ=255)

                                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                  >  1:4465392/1‑93 (MQ=255)

                                                                         tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGcc                <  1:672511/93‑1 (MQ=255)

                                                                           aCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCaa              >  2:222126/1‑93 (MQ=255)

                                                                             ggCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAcc            <  2:869949/93‑1 (MQ=255)

                                                                              gCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcg           >  1:1553072/1‑93 (MQ=255)

                                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc        >  2:4284766/1‑93 (MQ=255)

                                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc        >  2:3032443/1‑93 (MQ=255)

                                                                                  ggCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCt       <  1:2643479/93‑1 (MQ=255)

                                                                                     gTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa    <  1:2755287/93‑1 (MQ=255)

                                                                                     gTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa    <  2:766850/93‑1 (MQ=255)

                                                                                      tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt   <  1:2314579/93‑1 (MQ=255)

                                                                                      tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt   <  2:3074686/93‑1 (MQ=255)

                                                                                      tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt   >  1:560879/1‑93 (MQ=255)

                                                                                      tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt   >  2:929676/1‑93 (MQ=255)

TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT  >  NC_000913/3961498‑3961677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  >  NC_000913/3961495‑3961681

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACG                                                                                               >  SRR4302535.1912020/1‑94 (MQ=60)

 CTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGT                                                                                              >  SRR4302535.1800019/1‑94 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             >  SRR4302535.1384489/1‑94 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             >  SRR4302535.1058799/1‑94 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             >  SRR4302535.1825986/1‑94 (MQ=60)

   TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCG                                                                                            >  SRR4302535.4273848/1‑94 (MQ=60)

   TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCG                                                                                            <  SRR4302535.410958/94‑1 (MQ=60)

    CCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGC                                                                                           >  SRR4302535.2041881/1‑94 (MQ=60)

    CCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCACATTGACGGCAAGGCGTAAAGTCGC                                                                                           >  SRR4302535.924775/1‑94 (MQ=60)

     CTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCC                                                                                          >  SRR4302535.395576/1‑94 (MQ=60)

     CTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCC                                                                                          >  SRR4302535.1186177/1‑94 (MQ=60)

     CTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCC                                                                                          >  SRR4302535.556685/1‑94 (MQ=60)

      TCTTCGCCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCG                                                                                         <  SRR4302535.2666725/94‑1 (MQ=60)

       CTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGT                                                                                        >  SRR4302535.3929240/1‑94 (MQ=60)

       CTTCACCGCTGATCACCTGCACCGGACAACCGAGAATTTCCTGCCCGTTGGCAATAAAATCACCCGCATTGACGGCAAGCCGTAACGTCGCCGT                                                                                        >  SRR4302535.1560688/1‑94 (MQ=60)

         TCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAG                                                                                      <  SRR4302535.23707/94‑1 (MQ=60)

          CACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGC                                                                                     >  SRR4302535.1756213/1‑94 (MQ=60)

          CACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGC                                                                                     <  SRR4302535.2700215/94‑1 (MQ=60)

          CACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGC                                                                                     <  SRR4302535.4327605/94‑1 (MQ=60)

           ACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCG                                                                                    <  SRR4302535.461812/94‑1 (MQ=60)

           ACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCG                                                                                    <  SRR4302535.479963/94‑1 (MQ=60)

            CCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGA                                                                                   >  SRR4302535.978313/1‑94 (MQ=60)

            CCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGA                                                                                   >  SRR4302535.4235994/1‑94 (MQ=60)

             CGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAC                                                                                  >  SRR4302535.4539556/1‑94 (MQ=60)

             CGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAC                                                                                  >  SRR4302535.3406886/1‑94 (MQ=60)

              GCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACA                                                                                 >  SRR4302535.1238777/1‑94 (MQ=60)

               CTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAA                                                                                >  SRR4302535.3320270/1‑94 (MQ=60)

               CTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGAAACGTCGCCGTAGCacaaa                                                                                >  SRR4302535.3556358/1‑89 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               <  SRR4302535.4216179/94‑1 (MQ=60)

                 GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACA                                                                              <  SRR4302535.164132/94‑1 (MQ=60)

                  ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                             <  SRR4302535.3185530/94‑1 (MQ=60)

                  ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                             <  SRR4302535.3920063/94‑1 (MQ=60)

                    CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGA                                                                           <  SRR4302535.4029747/94‑1 (MQ=60)

                     ACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACacgat                                                                          >  SRR4302535.2894800/1‑89 (MQ=60)

                     ACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATAACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAA                                                                          >  SRR4302535.3263185/1‑94 (MQ=60)

                      CCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAT                                                                         <  SRR4302535.1067408/94‑1 (MQ=60)

                      CCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAT                                                                         >  SRR4302535.935865/1‑94 (MQ=60)

                       CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATT                                                                        <  SRR4302535.3311255/94‑1 (MQ=60)

                       CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATT                                                                        <  SRR4302535.3669569/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTT                                                                       <  SRR4302535.4315287/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTT                                                                       <  SRR4302535.3406880/94‑1 (MQ=60)

                         GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                      >  SRR4302535.4287892/1‑94 (MQ=60)

                         GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                      <  SRR4302535.3712929/94‑1 (MQ=60)

                         GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                      <  SRR4302535.3241702/94‑1 (MQ=60)

                          TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                     >  SRR4302535.171596/1‑94 (MQ=60)

                          TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                     <  SRR4302535.1088408/94‑1 (MQ=60)

                          TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                     <  SRR4302535.3461791/94‑1 (MQ=60)

                           ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                    <  SRR4302535.1231450/94‑1 (MQ=60)

                           ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                    <  SRR4302535.484342/94‑1 (MQ=60)

                           ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                    >  SRR4302535.2898690/1‑94 (MQ=60)

                             CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                  >  SRR4302535.1229273/1‑94 (MQ=60)

                             CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                  <  SRR4302535.1267512/94‑1 (MQ=60)

                             CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                  <  SRR4302535.2843214/94‑1 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                 >  SRR4302535.4444258/1‑94 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                 <  SRR4302535.306819/94‑1 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                 <  SRR4302535.130528/94‑1 (MQ=60)

                               GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGG                                                                <  SRR4302535.4493438/94‑1 (MQ=60)

                                ACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGA                                                               <  SRR4302535.1067913/94‑1 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                              >  SRR4302535.3596983/1‑94 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                              >  SRR4302535.2889256/1‑94 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                              <  SRR4302535.3442644/94‑1 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                              <  SRR4302535.1136301/94‑1 (MQ=60)

                                   ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                            >  SRR4302535.4476348/1‑94 (MQ=60)

                                    CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                           >  SRR4302535.1681194/1‑94 (MQ=60)

                                    CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                           >  SRR4302535.1138366/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                          >  SRR4302535.4671472/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                          >  SRR4302535.3855910/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                          <  SRR4302535.281830/94‑1 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                          >  SRR4302535.3505727/1‑94 (MQ=60)

                                      GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                         <  SRR4302535.2578087/94‑1 (MQ=60)

                                      GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                         >  SRR4302535.3265036/1‑94 (MQ=60)

                                       AGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGCATTTGCGAGGGAGGAATAT                                                        >  SRR4302535.154237/1‑94 (MQ=60)

                                       AGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATAT                                                        <  SRR4302535.1371705/94‑1 (MQ=60)

                                        GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                       <  SRR4302535.1272637/94‑1 (MQ=60)

                                        GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                       <  SRR4302535.2389047/94‑1 (MQ=60)

                                         AATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCT                                                      <  SRR4302535.1592421/94‑1 (MQ=60)

                                         AATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCT                                                      >  SRR4302535.1549579/1‑94 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                     >  SRR4302535.827016/1‑94 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                     >  SRR4302535.4432444/1‑94 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                     <  SRR4302535.4426827/94‑1 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                     <  SRR4302535.464953/94‑1 (MQ=60)

                                            TTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGC                                                   <  SRR4302535.2164710/94‑1 (MQ=60)

                                            TTCCTGCGCTTTGGCAATAAAACCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGCATTTGCGAGGGAGGAATATCTTGC                                                   >  SRR4302535.396141/1‑94 (MQ=60)

                                             TCCTGCGCTTTGGCAATAAAATCACCCGCATTGGCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCA                                                  <  SRR4302535.887388/94‑1 (MQ=60)

                                              TCTGCGCTTTGGCAATAAAATCCCCCGCATTGACGGCAAGGCGTACCGTCGCCGTCGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAG                                                 <  SRR4302535.987003/94‑1 (MQ=60)

                                               CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                <  SRR4302535.1713605/94‑1 (MQ=60)

                                               CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                <  SRR4302535.2219409/94‑1 (MQ=60)

                                                TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                               <  SRR4302535.3512298/94‑1 (MQ=60)

                                                 GCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACG                                              <  SRR4302535.3574870/94‑1 (MQ=60)

                                                  CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                             >  SRR4302535.4551333/1‑94 (MQ=60)

                                                  CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                             >  SRR4302535.4684442/1‑94 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            <  SRR4302535.3427885/94‑1 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            >  SRR4302535.4486347/1‑94 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            >  SRR4302535.1143289/1‑94 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            <  SRR4302535.3455375/94‑1 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            >  SRR4302535.1549883/1‑94 (MQ=60)

                                                    CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                           >  SRR4302535.1883693/1‑94 (MQ=60)

                                                    CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                           >  SRR4302535.4497863/1‑94 (MQ=60)

                                                     TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          <  SRR4302535.3705566/94‑1 (MQ=60)

                                                     TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          >  SRR4302535.2623271/1‑94 (MQ=60)

                                                     TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          <  SRR4302535.4709306/94‑1 (MQ=60)

                                                     TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          <  SRR4302535.1375779/94‑1 (MQ=60)

                                                      TTGGCAATAAACTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                         <  SRR4302535.2031102/94‑1 (MQ=60)

                                                      TTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                         <  SRR4302535.1192656/94‑1 (MQ=60)

                                                      TTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                         >  SRR4302535.3905310/1‑94 (MQ=60)

                                                         GCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAA                                      >  SRR4302535.2227516/1‑94 (MQ=60)

                                                          CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                     >  SRR4302535.3590772/1‑94 (MQ=60)

                                                          CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                     >  SRR4302535.2206676/1‑94 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    >  SRR4302535.1077773/1‑94 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   >  SRR4302535.4070101/1‑94 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   <  SRR4302535.3111349/94‑1 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGCCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   >  SRR4302535.654560/1‑94 (MQ=60)

                                                             TAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  <  SRR4302535.529083/94‑1 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  >  SRR4302535.4544592/1‑94 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  <  SRR4302535.4280509/94‑1 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  <  SRR4302535.3688095/94‑1 (MQ=60)

                                                              AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                 >  SRR4302535.484656/1‑94 (MQ=60)

                                                               AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                >  SRR4302535.127624/1‑94 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               >  SRR4302535.1010775/1‑94 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               >  SRR4302535.3752236/1‑94 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               <  SRR4302535.2498747/94‑1 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               <  SRR4302535.2953472/94‑1 (MQ=60)

                                                                 ATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGC                              >  SRR4302535.665207/1‑94 (MQ=60)

                                                                  TCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCA                             >  SRR4302535.4513380/1‑94 (MQ=60)

                                                                   CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                            >  SRR4302535.4672/1‑94 (MQ=60)

                                                                   CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                            >  SRR4302535.3574180/1‑94 (MQ=60)

                                                                    ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                           >  SRR4302535.3231476/1‑94 (MQ=60)

                                                                    ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                           >  SRR4302535.1427420/1‑94 (MQ=60)

                                                                     CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                          <  SRR4302535.468972/94‑1 (MQ=60)

                                                                       CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAT                        <  SRR4302535.4120446/94‑1 (MQ=60)

                                                                       CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGCTCAGAAAACCGGCGCAAACAT                        >  SRR4302535.797835/1‑94 (MQ=60)

                                                                        GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                       <  SRR4302535.3799531/94‑1 (MQ=60)

                                                                        GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                       <  SRR4302535.3554354/94‑1 (MQ=60)

                                                                         CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                      <  SRR4302535.683431/94‑1 (MQ=60)

                                                                         CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                      <  SRR4302535.678983/94‑1 (MQ=60)

                                                                         CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                      >  SRR4302535.4496021/1‑94 (MQ=60)

                                                                          ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     >  SRR4302535.2292638/1‑94 (MQ=60)

                                                                          ACTGACGGCAAGGCGTAACGTCGCCTTAGCGACAACACTAATTTGCGAGTGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     <  SRR4302535.1753912/94‑1 (MQ=60)

                                                                           TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                    <  SRR4302535.457062/94‑1 (MQ=60)

                                                                           TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                    <  SRR4302535.3043677/94‑1 (MQ=60)

                                                                           TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                    >  SRR4302535.897621/1‑94 (MQ=60)

                                                                           TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                    <  SRR4302535.1002474/94‑1 (MQ=60)

                                                                            TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                   <  SRR4302535.677205/94‑1 (MQ=60)

                                                                              ACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAC                 >  SRR4302535.3789851/1‑94 (MQ=60)

                                                                                GGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCG               >  SRR4302535.1564262/1‑94 (MQ=60)

                                                                                  CAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCG             >  SRR4302535.4511929/1‑94 (MQ=60)

                                                                                   AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC            >  SRR4302535.872786/1‑94 (MQ=60)

                                                                                    AGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT           >  SRR4302535.4390535/1‑94 (MQ=60)

                                                                                     GGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTC          <  SRR4302535.2662413/94‑1 (MQ=60)

                                                                                      GCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCC         <  SRR4302535.1785088/94‑1 (MQ=60)

                                                                                       CGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCA        >  SRR4302535.546270/1‑94 (MQ=60)

                                                                                        GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT       >  SRR4302535.564801/1‑94 (MQ=60)

                                                                                        GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT       <  SRR4302535.2774927/94‑1 (MQ=60)

                                                                                         TAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT      >  SRR4302535.1428935/1‑94 (MQ=60)

                                                                                         TAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT      <  SRR4302535.2331139/94‑1 (MQ=60)

                                                                                          AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG     >  SRR4302535.1295777/1‑94 (MQ=60)

                                                                                          AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG     >  SRR4302535.1049756/1‑94 (MQ=60)

                                                                                            CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT   >  SRR4302535.875138/1‑94 (MQ=60)

                                                                                            CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT   <  SRR4302535.1031341/94‑1 (MQ=60)

                                                                                             GTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  >  SRR4302535.1582941/1‑94 (MQ=60)

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  >  NC_000913/3961495‑3961681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`