Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,961,605 G→A R80R (CGC→CGT)  gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,961,6050GA99.8% 1682.7 / NA 468R80R (CGC→CGT) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (252/215);  minor base C (0/1);  total (252/216)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC  >  NC_000913/3961514‑3961694
                                                                                           |                                                                                         
 cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcacg                                                                                         <  2:3312859/93‑1 (MQ=255)
   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       <  2:1380788/93‑1 (MQ=255)
      gTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAttt                                                                                    >  2:2441695/1‑93 (MQ=255)
          cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCga                                                                                <  1:7080667/93‑1 (MQ=255)
          cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCga                                                                                <  1:5174816/93‑1 (MQ=255)
           ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                                               >  1:3635758/1‑93 (MQ=255)
            gACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgcgg                                                                              >  1:6545075/1‑93 (MQ=255)
            gACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagg                                                                              >  1:6822645/1‑93 (MQ=255)
              cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgaggga                                                                            >  1:7198210/1‑93 (MQ=255)
               aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggag                                                                           >  2:4365217/1‑93 (MQ=255)
               aaCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggag                                                                           >  1:7584858/1‑93 (MQ=255)
                aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggagg                                                                          >  2:7380429/1‑93 (MQ=255)
                aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggagg                                                                          <  1:3524161/93‑1 (MQ=255)
                 ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGggagga                                                                         >  1:1013133/1‑93 (MQ=255)
                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                                        >  1:2267419/1‑93 (MQ=255)
                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                                        <  2:2767479/93‑1 (MQ=255)
                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                                        >  2:6214274/1‑93 (MQ=255)
                   gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  1:2223952/1‑93 (MQ=255)
                   gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       <  1:6750482/93‑1 (MQ=255)
                   gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  1:1914588/1‑93 (MQ=255)
                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAatatt                                                                    >  1:1652577/1‑92 (MQ=255)
                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                                    >  1:3972928/1‑93 (MQ=255)
                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                                    <  2:3415253/93‑1 (MQ=255)
                      aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                                    >  1:7478778/1‑93 (MQ=255)
                        tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCtt                                                                  >  2:2679861/1‑93 (MQ=255)
                        tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCtt                                                                  >  1:4481595/1‑93 (MQ=255)
                         ttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTg                                                                 >  2:3815402/1‑93 (MQ=255)
                          tCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGc                                                                <  2:3646773/93‑1 (MQ=255)
                           ccTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCa                                                               <  2:5117429/93‑1 (MQ=255)
                            ccgcgcTTTGGCAATAAAACCACCCGCATGGACGGCAAGGCGTAACGACGCCGTAGCGACAACACGAACGCGCGAGGGAGGAATATCTTGCAg                                                              >  1:7008716/3‑93 (MQ=255)
                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGa                                                             >  1:5022833/1‑93 (MQ=255)
                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGa                                                             <  2:707317/93‑1 (MQ=255)
                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGa                                                             >  2:1524532/1‑93 (MQ=255)
                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGa                                                             <  1:3097384/93‑1 (MQ=255)
                              gcgcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAc                                                            >  1:1300975/1‑93 (MQ=255)
                               cgcTTTGGCAATAAAATCACCCGCATGGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACg                                                           <  2:4865638/93‑1 (MQ=255)
                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACACCACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          <  2:5986340/93‑1 (MQ=255)
                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          >  1:2429965/1‑93 (MQ=255)
                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          >  1:6511148/1‑93 (MQ=255)
                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          >  1:2592877/1‑93 (MQ=255)
                                 cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGtt                                                         >  2:414166/1‑93 (MQ=255)
                                    tGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAg                                                      <  2:3074490/93‑1 (MQ=255)
                                        aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                                  >  1:2943123/1‑93 (MQ=255)
                                        aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                                  >  1:4639673/1‑93 (MQ=255)
                                        aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                                  <  2:637108/93‑1 (MQ=255)
                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 <  1:237393/93‑1 (MQ=255)
                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 >  1:347032/1‑93 (MQ=255)
                                         aTAAAATCACCCGCATTGACGGCAAGGAGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 >  1:3300447/1‑93 (MQ=255)
                                          tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGTAAACa                                                >  2:4398997/1‑93 (MQ=255)
                                          tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                <  1:5969212/93‑1 (MQ=255)
                                          tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                <  1:1514962/93‑1 (MQ=255)
                                           aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg                                               <  2:217405/93‑1 (MQ=255)
                                            aaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAgg                                              <  1:5854747/93‑1 (MQ=255)
                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                                             <  2:2532812/93‑1 (MQ=255)
                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                                             >  2:7551778/1‑93 (MQ=255)
                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                                             >  2:2284614/1‑93 (MQ=255)
                                               tCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcgc                                           >  1:5996226/1‑93 (MQ=255)
                                                  cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                                        >  2:813511/1‑93 (MQ=255)
                                                   ccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAc                                       <  2:4709302/93‑1 (MQ=255)
                                                   ccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAc                                       <  1:4956654/93‑1 (MQ=255)
                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt                                     >  2:4319658/1‑93 (MQ=255)
                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt                                     <  1:946806/93‑1 (MQ=255)
                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                                   >  2:175396/1‑93 (MQ=255)
                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                                   >  2:6740844/1‑93 (MQ=255)
                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                                   <  1:2153929/93‑1 (MQ=255)
                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                                   >  2:5783460/1‑93 (MQ=255)
                                                        ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGATGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                                  >  1:5680959/1‑93 (MQ=255)
                                                         tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAACCAGGCGCAAACATTGca                                 >  2:4020516/1‑92 (MQ=255)
                                                         tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGcc                                 <  1:4537476/93‑1 (MQ=255)
                                                          gACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCa                                >  1:771529/1‑93 (MQ=255)
                                                          gACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCa                                >  1:1708273/1‑93 (MQ=255)
                                                           aCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCaa                               <  1:274465/93‑1 (MQ=255)
                                                               cAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAcccg                           >  2:7694162/1‑91 (MQ=255)
                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcgtgt                         >  2:7238243/1‑90 (MQ=255)
                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         <  1:5054153/93‑1 (MQ=255)
                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         >  2:4937790/1‑93 (MQ=255)
                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         >  2:189531/1‑93 (MQ=255)
                                                                 aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         <  1:6242595/93‑1 (MQ=255)
                                                                  ggCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCt                        >  2:3008152/1‑93 (MQ=255)
                                                                   gCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTc                       >  1:3822719/1‑93 (MQ=255)
                                                                   gCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTc                       >  2:2884683/1‑93 (MQ=255)
                                                                     gTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa                     <  2:1980322/93‑1 (MQ=255)
                                                                       aaCGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAtt                   <  2:7484412/93‑1 (MQ=255)
                                                                           tCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCtt               <  2:703657/93‑1 (MQ=255)
                                                                           tCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCtt               >  2:6074126/1‑93 (MQ=255)
                                                                             gCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCa             <  1:5329308/93‑1 (MQ=255)
                                                                             gCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCa             <  1:7887344/93‑1 (MQ=255)
                                                                                 tAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTgg         >  1:2065526/1‑93 (MQ=255)
                                                                                 tAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTgg         <  1:6177109/93‑1 (MQ=255)
                                                                                  aGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGga        <  2:4595813/93‑1 (MQ=255)
                                                                                  aGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGga        <  2:741026/93‑1 (MQ=255)
                                                                                    cGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGgaga      <  2:5739335/93‑1 (MQ=255)
                                                                                    cGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGgaga      <  2:5448091/93‑1 (MQ=255)
                                                                                     gACAGCACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGagag     <  1:1202707/93‑1 (MQ=255)
                                                                                     gACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGagag     >  2:1145032/1‑93 (MQ=255)
                                                                                      acaacaCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAgg    >  2:1837219/1‑93 (MQ=255)
                                                                                      acaacaCGAATTTGCGAGCGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAgg    <  1:6079938/93‑1 (MQ=255)
                                                                                       cAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAggg   <  2:4211783/93‑1 (MQ=255)
                                                                                       cAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAggg   >  2:7833685/1‑93 (MQ=255)
                                                                                        aaCACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGc  >  2:5291694/1‑93 (MQ=255)
                                                                                           |                                                                                         
TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC  >  NC_000913/3961514‑3961694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 263 total aligned reads displayed.
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  >  NC_000913/3961512‑3961698
                                                                                             |                                                                                             
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACA                                                                                               >  SRR4302543.7578034/1‑94 (MQ=60)
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACA                                                                                               >  SRR4302543.2893402/1‑94 (MQ=60)
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACA                                                                                               <  SRR4302543.1488080/94‑1 (MQ=60)
 ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                                              >  SRR4302543.6091113/1‑94 (MQ=60)
 ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                                              >  SRR4302543.4084408/1‑94 (MQ=60)
 ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                                              <  SRR4302543.6824709/94‑1 (MQ=60)
  TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACG                                                                                             <  SRR4302543.2265620/94‑1 (MQ=60)
  TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACG                                                                                             <  SRR4302543.2910674/94‑1 (MQ=60)
  TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACG                                                                                             <  SRR4302543.731601/94‑1 (MQ=60)
   CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGA                                                                                            >  SRR4302543.659399/1‑94 (MQ=60)
   CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGA                                                                                            >  SRR4302543.6773161/1‑94 (MQ=60)
    ACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAA                                                                                           <  SRR4302543.6576132/94‑1 (MQ=60)
     CCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAT                                                                                          >  SRR4302543.278689/1‑94 (MQ=60)
      CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATT                                                                                         >  SRR4302543.6561476/1‑94 (MQ=60)
       TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTT                                                                                        >  SRR4302543.2048985/1‑94 (MQ=60)
         TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                                      >  SRR4302543.7779732/1‑94 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302543.1630631/94‑1 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   >  SRR4302543.3667754/1‑94 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302543.7149429/94‑1 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302543.7149427/94‑1 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302543.5221541/94‑1 (MQ=60)
             GGACAACCGATAATTTCCTGCGCTTTGGCAATAAAATCACCCGTATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302543.1676481/94‑1 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGCGG                                                                                  >  SRR4302543.6606676/1‑94 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302543.1766040/94‑1 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302543.5008585/94‑1 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  >  SRR4302543.6887055/1‑94 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302543.6273529/94‑1 (MQ=60)
              GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGG                                                                                 <  SRR4302543.1501600/94‑1 (MQ=60)
              GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGG                                                                                 <  SRR4302543.152060/94‑1 (MQ=60)
              GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGG                                                                                 <  SRR4302543.6007521/94‑1 (MQ=60)
               ACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGA                                                                                >  SRR4302543.7268001/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302543.6271065/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302543.6139396/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302543.7656946/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               <  SRR4302543.3809733/94‑1 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302543.7020423/1‑94 (MQ=60)
                 AACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGG                                                                              <  SRR4302543.5083540/94‑1 (MQ=60)
                  ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                                             >  SRR4302543.1022099/1‑94 (MQ=60)
                  ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                                             <  SRR4302543.3555587/94‑1 (MQ=60)
                  ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                                             <  SRR4302543.5629398/94‑1 (MQ=60)
                  ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                                             <  SRR4302543.3688671/94‑1 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGACTTTGCGAGGGAGGAA                                                                            >  SRR4302543.3956387/1‑94 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                                            <  SRR4302543.6429083/94‑1 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                                            >  SRR4302543.2288123/1‑94 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                                            <  SRR4302543.3539242/94‑1 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           <  SRR4302543.4657963/94‑1 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302543.1931847/1‑94 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           <  SRR4302543.6839665/94‑1 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302543.3418386/1‑94 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302543.2244028/1‑94 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          <  SRR4302543.5264218/94‑1 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          >  SRR4302543.2706173/1‑94 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          <  SRR4302543.6814231/94‑1 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          <  SRR4302543.710281/94‑1 (MQ=60)
                      AGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATAT                                                                         <  SRR4302543.3495890/94‑1 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATT                                                                        >  SRR4302543.1667359/1‑94 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                                        <  SRR4302543.190487/94‑1 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                                        >  SRR4302543.4008146/1‑94 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                                        >  SRR4302543.7550371/1‑94 (MQ=60)
                        AATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCT                                                                       >  SRR4302543.7985956/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCTTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302543.4522296/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302543.4522295/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302543.2041577/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302543.5106324/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATATT                                                                      <  SRR4302543.3603171/94‑1 (MQ=60)
                          TTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTG                                                                     <  SRR4302543.3015059/94‑1 (MQ=60)
                          TTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTG                                                                     <  SRR4302543.2438205/94‑1 (MQ=60)
                            TCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCA                                                                   <  SRR4302543.6152740/94‑1 (MQ=60)
                             CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAG                                                                  >  SRR4302543.6071497/1‑94 (MQ=60)
                             CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAG                                                                  >  SRR4302543.2697667/1‑94 (MQ=60)
                             CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAG                                                                  >  SRR4302543.5604419/1‑94 (MQ=60)
                              CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                                 >  SRR4302543.5759120/1‑94 (MQ=60)
                              CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                                 >  SRR4302543.5762628/1‑94 (MQ=60)
                              CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                                 >  SRR4302543.5068543/1‑94 (MQ=60)
                               tgcgcggtgtcaataaaatcgcccgcagcaACGGCAAGGCGTAACGCCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302543.4046088/64‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302543.3125017/94‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302543.6072007/94‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302543.7835519/94‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302543.3125241/94‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                >  SRR4302543.1312534/1‑94 (MQ=60)
                                GCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACG                                                               >  SRR4302543.27931/1‑94 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              >  SRR4302543.2616434/1‑94 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              >  SRR4302543.7504566/1‑94 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              >  SRR4302543.7565132/1‑94 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              <  SRR4302543.2751081/94‑1 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             >  SRR4302543.2195585/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACCACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302543.4713909/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302543.2432461/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302543.6176331/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302543.1600836/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            <  SRR4302543.503468/94‑1 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           >  SRR4302543.2389454/1‑94 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           >  SRR4302543.2550258/1‑94 (MQ=60)
                                       GGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCA                                                        <  SRR4302543.1783834/94‑1 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      >  SRR4302543.7884009/1‑94 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      >  SRR4302543.4681866/1‑94 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      >  SRR4302543.2969844/1‑94 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      <  SRR4302543.5643873/94‑1 (MQ=60)
                                          AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                                     <  SRR4302543.6016944/94‑1 (MQ=60)
                                          AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                                     >  SRR4302543.7783669/1‑94 (MQ=60)
                                          AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                                     >  SRR4302543.4549226/1‑94 (MQ=60)
                                          AATAAAATCACCCGCATTGACGGCAAGGAGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                                     >  SRR4302543.3330233/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    >  SRR4302543.476541/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    >  SRR4302543.7751036/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    >  SRR4302543.6136978/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    <  SRR4302543.239447/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302543.3407455/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302543.6023873/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302543.2130529/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302543.1160193/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   >  SRR4302543.4638428/1‑94 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302543.411725/94‑1 (MQ=60)
                                             AAAATCACCCGCATTGACGGCAAGGCTTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                                  <  SRR4302543.7974858/94‑1 (MQ=60)
                                             AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                                  >  SRR4302543.6029564/1‑94 (MQ=60)
                                             AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                                  >  SRR4302543.6029563/1‑94 (MQ=60)
                                              AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                                 <  SRR4302543.5908035/94‑1 (MQ=60)
                                              AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                                 <  SRR4302543.6418494/94‑1 (MQ=60)
                                              AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                                 <  SRR4302543.7256384/94‑1 (MQ=60)
                                              AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                                 <  SRR4302543.5953988/94‑1 (MQ=60)
                                                   ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                                            >  SRR4302543.7637122/1‑94 (MQ=60)
                                                   ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                                            >  SRR4302543.2910856/1‑94 (MQ=60)
                                                     CCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACA                                          <  SRR4302543.5001780/94‑1 (MQ=60)
                                                      CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAT                                         >  SRR4302543.1215676/1‑94 (MQ=60)
                                                       GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        >  SRR4302543.590979/1‑94 (MQ=60)
                                                       GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        <  SRR4302543.955078/94‑1 (MQ=60)
                                                       GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        <  SRR4302543.2333859/94‑1 (MQ=60)
                                                       CCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        <  SRR4302543.6811027/94‑1 (MQ=60)
                                                        CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                                       >  SRR4302543.5151120/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGATGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.5732813/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.6930379/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.3526109/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      <  SRR4302543.2173218/94‑1 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      <  SRR4302543.3017031/94‑1 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.2139509/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.3977509/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.4565929/1‑94 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302543.675218/1‑94 (MQ=60)
                                                         ATGGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      <  SRR4302543.2173219/94‑1 (MQ=60)
                                                          ctctCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     <  SRR4302543.2485344/90‑1 (MQ=60)
                                                          TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     <  SRR4302543.2107514/94‑1 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    >  SRR4302543.1723561/1‑94 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    >  SRR4302543.84376/1‑94 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    >  SRR4302543.2232425/1‑94 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    >  SRR4302543.778296/1‑94 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    <  SRR4302543.6869061/94‑1 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    >  SRR4302543.7209084/1‑94 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    <  SRR4302543.4578781/94‑1 (MQ=60)
                                                             ACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAC                                  <  SRR4302543.276885/94‑1 (MQ=60)
                                                              CGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGTAAACAGGCGCAAACATTGCCAACC                                 <  SRR4302543.1576669/94‑1 (MQ=60)
                                                               GGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGGACACAGGCGCAAACATTGCCAACCG                                >  SRR4302543.2551011/1‑94 (MQ=60)
                                                               GGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCG                                >  SRR4302543.3866512/1‑94 (MQ=60)
                                                                GCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGC                               <  SRR4302543.965204/94‑1 (MQ=60)
                                                                 CAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCG                              >  SRR4302543.4805734/1‑94 (MQ=60)
                                                                  AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC                             >  SRR4302543.3772204/1‑94 (MQ=60)
                                                                  AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC                             >  SRR4302543.5338286/1‑94 (MQ=60)
                                                                  AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC                             >  SRR4302543.6039758/1‑94 (MQ=60)
                                                                  AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC                             >  SRR4302543.5830662/1‑94 (MQ=60)
                                                                   AGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT                            <  SRR4302543.6301489/94‑1 (MQ=60)
                                                                   AGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT                            <  SRR4302543.5100102/94‑1 (MQ=60)
                                                                   AGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT                            >  SRR4302543.3131134/1‑94 (MQ=60)
                                                                    GGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTC                           >  SRR4302543.3856434/1‑94 (MQ=60)
                                                                     GCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCC                          <  SRR4302543.6589009/94‑1 (MQ=60)
                                                                      CGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCA                         >  SRR4302543.2030166/1‑94 (MQ=60)
                                                                      CGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCA                         >  SRR4302543.6893454/1‑94 (MQ=60)
                                                                       GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT                        >  SRR4302543.3328362/1‑94 (MQ=60)
                                                                       GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT                        <  SRR4302543.72473/94‑1 (MQ=60)
                                                                       GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT                        >  SRR4302543.1351519/1‑94 (MQ=60)
                                                                        TAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT                       <  SRR4302543.5192443/94‑1 (MQ=60)
                                                                         AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG                      <  SRR4302543.5080439/94‑1 (MQ=60)
                                                                         AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG                      <  SRR4302543.3558098/94‑1 (MQ=60)
                                                                           CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT                    <  SRR4302543.2421338/94‑1 (MQ=60)
                                                                           CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGACAACCGCGCTCCATTGCT                    >  SRR4302543.3474571/1‑94 (MQ=60)
                                                                            GTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT                   >  SRR4302543.1918068/1‑94 (MQ=60)
                                                                            GTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT                   >  SRR4302543.3611860/1‑94 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302543.2256776/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302543.7454443/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302543.7961542/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302543.5377503/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302543.1253543/94‑1 (MQ=60)
                                                                                catTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATT               <  SRR4302543.1147219/91‑1 (MQ=60)
                                                                                CCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATT               <  SRR4302543.7839887/94‑1 (MQ=60)
                                                                                CCGTAGCGACAACACGAATGTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATT               <  SRR4302543.1308876/94‑1 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302543.1683608/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302543.7490610/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302543.7555007/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              <  SRR4302543.2318590/94‑1 (MQ=60)
                                                                                   TAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGA            <  SRR4302543.6235513/94‑1 (MQ=60)
                                                                                    AGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAG           >  SRR4302543.5058285/1‑94 (MQ=60)
                                                                                      CGACAACACGAATTTGCGAGGGAGGAATATCTAGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAG         <  SRR4302543.7077815/94‑1 (MQ=60)
                                                                                       GACAGCACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGG        <  SRR4302543.1213536/94‑1 (MQ=60)
                                                                                       GACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGG        <  SRR4302543.5367436/94‑1 (MQ=60)
                                                                                        ACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGG       <  SRR4302543.2259572/94‑1 (MQ=60)
                                                                                        ACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGG       >  SRR4302543.1698658/1‑94 (MQ=60)
                                                                                        ACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGG       <  SRR4302543.6839462/94‑1 (MQ=60)
                                                                                        ACAACACGAATTTGCGAGCGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGG       <  SRR4302543.6137555/94‑1 (MQ=60)
                                                                                         CAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC      >  SRR4302543.3207185/1‑94 (MQ=60)
                                                                                          AACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCA     <  SRR4302543.1056569/94‑1 (MQ=60)
                                                                                          AACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCCCGCTCCATTGCTTCATTGGggggggct     >  SRR4302543.6173416/1‑86 (MQ=60)
                                                                                           ACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCAT    >  SRR4302543.4517419/1‑94 (MQ=60)
                                                                                             ACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  >  SRR4302543.4159756/1‑94 (MQ=60)
                                                                                             |                                                                                             
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  >  NC_000913/3961512‑3961698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑