Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,961,605 G→A R80R (CGC→CGT)  gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,961,6050GA99.3% 1014.7 / ‑5.8 284R80R (CGC→CGT) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (150/130);  total (150/134)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC  >  NC_000913/3961515‑3961694
                                                                                          |                                                                                         
cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcacg                                                                                         >  2:4631677/1‑93 (MQ=255)
cACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcacg                                                                                         >  2:1858308/1‑93 (MQ=255)
 aCCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGa                                                                                        >  2:2725053/1‑93 (MQ=255)
  ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       <  2:4482535/93‑1 (MQ=255)
  ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       <  2:4310698/93‑1 (MQ=255)
  ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       <  2:3851943/93‑1 (MQ=255)
  ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       >  2:4148505/1‑93 (MQ=255)
  ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATAACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGaa                                                                                       >  1:3240684/1‑93 (MQ=255)
   cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAt                                                                                      >  2:2944025/1‑93 (MQ=255)
   cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAt                                                                                      <  2:946674/93‑1 (MQ=255)
    tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                                     <  1:4285967/93‑1 (MQ=255)
    tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                                     <  2:2365705/93‑1 (MQ=255)
    tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                                     <  2:1676372/93‑1 (MQ=255)
    tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAtt                                                                                     <  1:3383359/93‑1 (MQ=255)
     gTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCCCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAttt                                                                                    <  2:3084011/93‑1 (MQ=255)
     gTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAttt                                                                                    <  2:4266244/93‑1 (MQ=255)
        ccGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCg                                                                                 >  1:2878307/1‑93 (MQ=255)
         cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCga                                                                                <  2:1513018/93‑1 (MQ=255)
         cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCga                                                                                <  2:523688/93‑1 (MQ=255)
          ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                                               >  1:1220525/1‑93 (MQ=255)
          ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                                               >  2:3180104/1‑93 (MQ=255)
          ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                                               <  1:129615/93‑1 (MQ=255)
          ggACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgag                                                                               <  2:2165732/93‑1 (MQ=255)
             cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgaggga                                                                            >  2:3777800/1‑93 (MQ=255)
               aCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCgagggagg                                                                          <  2:1975730/93‑1 (MQ=255)
                 cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                                        >  1:1669117/1‑93 (MQ=255)
                 cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGaa                                                                        >  1:1130298/1‑93 (MQ=255)
                  gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  1:3481546/1‑93 (MQ=255)
                  gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  2:2644161/1‑93 (MQ=255)
                  gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  2:92215/1‑93 (MQ=255)
                  gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  2:4546883/1‑93 (MQ=255)
                  gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAat                                                                       >  1:3829499/1‑93 (MQ=255)
                    gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAatat                                                                     <  1:2372010/93‑1 (MQ=255)
                    gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAatat                                                                     <  1:1263596/93‑1 (MQ=255)
                     aaTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                                    <  2:3733573/93‑1 (MQ=255)
                     aaTTCCCTGCGCTTTGACAATAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATc                                                                    <  2:3508891/93‑1 (MQ=255)
                      aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                                   <  2:477205/93‑1 (MQ=255)
                      aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                                   <  1:461728/93‑1 (MQ=255)
                      aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                                   >  2:180407/1‑93 (MQ=255)
                      aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCt                                                                   >  1:1538491/1‑93 (MQ=255)
                       tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCtt                                                                  >  1:4402318/1‑93 (MQ=255)
                        ttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTg                                                                 <  1:2149255/93‑1 (MQ=255)
                         tCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGc                                                                <  2:3952693/93‑1 (MQ=255)
                          ccTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCa                                                               <  2:715693/93‑1 (MQ=255)
                           cTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAg                                                              <  1:2203708/93‑1 (MQ=255)
                               gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          <  1:3431526/93‑1 (MQ=255)
                               gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                                          >  1:4652938/1‑93 (MQ=255)
                                cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGtt                                                         >  1:4455790/1‑93 (MQ=255)
                                 tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc                                                        <  1:4677740/93‑1 (MQ=255)
                                 tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc                                                        >  1:4467214/1‑93 (MQ=255)
                                 tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGGCGCCGTAGCGACAACCCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc                                                        <  2:2605363/93‑1 (MQ=255)
                                  ttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCa                                                       <  2:4681730/93‑1 (MQ=255)
                                       aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                                  >  1:3566048/1‑93 (MQ=255)
                                        aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGGCAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 >  2:3712592/1‑93 (MQ=255)
                                        aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 >  1:1070166/1‑93 (MQ=255)
                                        aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                                                 >  2:2873706/1‑93 (MQ=255)
                                         tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                <  2:3036008/93‑1 (MQ=255)
                                         tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                >  1:4042426/1‑93 (MQ=255)
                                         tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                <  1:3662734/93‑1 (MQ=255)
                                         tAAAATCACCCGCATTGACGGCAAGGCGTAACGCCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                                                >  1:650027/1‑93 (MQ=255)
                                          aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg                                               >  1:4513640/1‑93 (MQ=255)
                                           aaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAgg                                              >  1:481282/1‑93 (MQ=255)
                                             aTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcg                                            >  1:1003650/1‑93 (MQ=255)
                                             aTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgcg                                            >  1:3726420/1‑93 (MQ=255)
                                                aCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaa                                         >  1:3549545/1‑93 (MQ=255)
                                                 cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                                        >  1:1417301/1‑93 (MQ=255)
                                                 cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                                        >  2:3116911/1‑93 (MQ=255)
                                                     cATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAtt                                    <  1:674273/93‑1 (MQ=255)
                                                       ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                                  <  1:3022515/93‑1 (MQ=255)
                                                       ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                                  >  2:3438619/1‑93 (MQ=255)
                                                       ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                                  >  1:2276383/1‑93 (MQ=255)
                                                        tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGcc                                 >  1:891304/1‑93 (MQ=255)
                                                           cGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAc                              >  2:1136091/1‑93 (MQ=255)
                                                            ggCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAcc                             <  2:869949/93‑1 (MQ=255)
                                                                aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         >  1:866657/1‑93 (MQ=255)
                                                                aGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc                         >  2:4109188/1‑93 (MQ=255)
                                                                  gCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTc                       <  1:1772247/93‑1 (MQ=255)
                                                                    gTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa                     <  1:2755287/93‑1 (MQ=255)
                                                                    gTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa                     >  1:542479/1‑93 (MQ=255)
                                                                     tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt                    >  2:676030/1‑93 (MQ=255)
                                                                     tAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt                    >  2:1660001/1‑93 (MQ=255)
                                                                       acgtCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTg                  >  1:1042367/4‑93 (MQ=255)
                                                                        cgtCGCCGGAGCGACAACACGAATTTGCGAGGCAGGAATATCTTCCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGc                 <  2:1246556/91‑1 (MQ=255)
                                                                          tCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCtt               >  1:1571596/1‑93 (MQ=255)
                                                                          tCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCtt               >  2:2495452/1‑93 (MQ=255)
                                                                            gCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCa             >  1:3093066/1‑93 (MQ=255)
                                                                            gCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCa             <  1:4251112/93‑1 (MQ=255)
                                                                             ccGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAt            <  2:2800192/93‑1 (MQ=255)
                                                                              cGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAtt           <  2:1225016/93‑1 (MQ=255)
                                                                              cGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAtt           >  1:710620/1‑93 (MQ=255)
                                                                               gTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCGGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTg          >  1:2606218/1‑93 (MQ=255)
                                                                                tAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTgg         >  1:2589759/1‑93 (MQ=255)
                                                                                 aGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGga        >  2:2247196/1‑93 (MQ=255)
                                                                                 aGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGga        <  1:171364/93‑1 (MQ=255)
                                                                                  gCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGgag       >  2:1607863/1‑93 (MQ=255)
                                                                                   cGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGgaga      <  1:2852208/93‑1 (MQ=255)
                                                                                    gACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGagag     >  2:4038555/1‑93 (MQ=255)
                                                                                    gACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGagag     <  2:3328/93‑1 (MQ=255)
                                                                                      cAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAggg   <  2:1010019/93‑1 (MQ=255)
                                                                                       aaCACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGc  >  2:1333149/1‑93 (MQ=255)
                                                                                          |                                                                                         
CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC  >  NC_000913/3961515‑3961694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  >  NC_000913/3961512‑3961698
                                                                                             |                                                                                             
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACA                                                                                               <  SRR4302535.164132/94‑1 (MQ=60)
 ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                                              <  SRR4302535.3185530/94‑1 (MQ=60)
 ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACAC                                                                                              <  SRR4302535.3920063/94‑1 (MQ=60)
   CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGA                                                                                            <  SRR4302535.4029747/94‑1 (MQ=60)
    ACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATAACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAA                                                                                           >  SRR4302535.3263185/1‑94 (MQ=60)
     CCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAT                                                                                          >  SRR4302535.935865/1‑94 (MQ=60)
     CCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAAT                                                                                          <  SRR4302535.1067408/94‑1 (MQ=60)
      CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATT                                                                                         <  SRR4302535.3669569/94‑1 (MQ=60)
      CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATT                                                                                         <  SRR4302535.3311255/94‑1 (MQ=60)
       TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTT                                                                                        <  SRR4302535.3406880/94‑1 (MQ=60)
       TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTT                                                                                        <  SRR4302535.4315287/94‑1 (MQ=60)
        GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                                       <  SRR4302535.3712929/94‑1 (MQ=60)
        GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                                       >  SRR4302535.4287892/1‑94 (MQ=60)
        GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTG                                                                                       <  SRR4302535.3241702/94‑1 (MQ=60)
         TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                                      <  SRR4302535.1088408/94‑1 (MQ=60)
         TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                                      >  SRR4302535.171596/1‑94 (MQ=60)
         TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGC                                                                                      <  SRR4302535.3461791/94‑1 (MQ=60)
          ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                                     >  SRR4302535.2898690/1‑94 (MQ=60)
          ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                                     <  SRR4302535.484342/94‑1 (MQ=60)
          ACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCG                                                                                     <  SRR4302535.1231450/94‑1 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302535.1267512/94‑1 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   >  SRR4302535.1229273/1‑94 (MQ=60)
            CGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAG                                                                                   <  SRR4302535.2843214/94‑1 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302535.130528/94‑1 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  >  SRR4302535.4444258/1‑94 (MQ=60)
             GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGG                                                                                  <  SRR4302535.306819/94‑1 (MQ=60)
              GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGG                                                                                 <  SRR4302535.4493438/94‑1 (MQ=60)
               ACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGA                                                                                <  SRR4302535.1067913/94‑1 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               <  SRR4302535.3442644/94‑1 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302535.2889256/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               >  SRR4302535.3596983/1‑94 (MQ=60)
                CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAG                                                                               <  SRR4302535.1136301/94‑1 (MQ=60)
                  ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGA                                                                             >  SRR4302535.4476348/1‑94 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                                            >  SRR4302535.1681194/1‑94 (MQ=60)
                   CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAA                                                                            >  SRR4302535.1138366/1‑94 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302535.3505727/1‑94 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           <  SRR4302535.281830/94‑1 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302535.4671472/1‑94 (MQ=60)
                    CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAAT                                                                           >  SRR4302535.3855910/1‑94 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          <  SRR4302535.2578087/94‑1 (MQ=60)
                     GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATA                                                                          >  SRR4302535.3265036/1‑94 (MQ=60)
                      AGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGCATTTGCGAGGGAGGAATAT                                                                         >  SRR4302535.154237/1‑94 (MQ=60)
                      AGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATAT                                                                         <  SRR4302535.1371705/94‑1 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                                        <  SRR4302535.1272637/94‑1 (MQ=60)
                       GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATC                                                                        <  SRR4302535.2389047/94‑1 (MQ=60)
                        AATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCT                                                                       <  SRR4302535.1592421/94‑1 (MQ=60)
                        AATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCT                                                                       >  SRR4302535.1549579/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      <  SRR4302535.4426827/94‑1 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      <  SRR4302535.464953/94‑1 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302535.4432444/1‑94 (MQ=60)
                         ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTT                                                                      >  SRR4302535.827016/1‑94 (MQ=60)
                           TTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGC                                                                    <  SRR4302535.2164710/94‑1 (MQ=60)
                           TTCCTGCGCTTTGGCAATAAAACCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGCATTTGCGAGGGAGGAATATCTTGC                                                                    >  SRR4302535.396141/1‑94 (MQ=60)
                            TCCTGCGCTTTGGCAATAAAATCACCCGCATTGGCGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCA                                                                   <  SRR4302535.887388/94‑1 (MQ=60)
                             TCTGCGCTTTGGCAATAAAATCCCCCGCATTGACGGCAAGGCGTACCGTCGCCGTCGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAG                                                                  <  SRR4302535.987003/94‑1 (MQ=60)
                              CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                                 <  SRR4302535.2219409/94‑1 (MQ=60)
                              CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGA                                                                 <  SRR4302535.1713605/94‑1 (MQ=60)
                               TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGAC                                                                <  SRR4302535.3512298/94‑1 (MQ=60)
                                GCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACG                                                               <  SRR4302535.3574870/94‑1 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              >  SRR4302535.4551333/1‑94 (MQ=60)
                                 CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCATAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                                              >  SRR4302535.4684442/1‑94 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             <  SRR4302535.3455375/94‑1 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             <  SRR4302535.3427885/94‑1 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             >  SRR4302535.1143289/1‑94 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             >  SRR4302535.4486347/1‑94 (MQ=60)
                                  GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                                             >  SRR4302535.1549883/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302535.1883693/1‑94 (MQ=60)
                                   CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                                            >  SRR4302535.4497863/1‑94 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           >  SRR4302535.2623271/1‑94 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           <  SRR4302535.3705566/94‑1 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           <  SRR4302535.1375779/94‑1 (MQ=60)
                                    TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                                           <  SRR4302535.4709306/94‑1 (MQ=60)
                                     TTGGCAATAAACTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                                          <  SRR4302535.2031102/94‑1 (MQ=60)
                                     TTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                                          >  SRR4302535.3905310/1‑94 (MQ=60)
                                     TTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAG                                                          <  SRR4302535.1192656/94‑1 (MQ=60)
                                        GCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAA                                                       >  SRR4302535.2227516/1‑94 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      >  SRR4302535.3590772/1‑94 (MQ=60)
                                         CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                                      >  SRR4302535.2206676/1‑94 (MQ=60)
                                          AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                                     >  SRR4302535.1077773/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    <  SRR4302535.3111349/94‑1 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    >  SRR4302535.4070101/1‑94 (MQ=60)
                                           ATAAAATCACCCGCATTGACGGCAAGGCGTAACGCCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                                    >  SRR4302535.654560/1‑94 (MQ=60)
                                            TAAAATCGCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302535.529083/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302535.4280509/94‑1 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   >  SRR4302535.4544592/1‑94 (MQ=60)
                                            TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                                   <  SRR4302535.3688095/94‑1 (MQ=60)
                                             AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                                  >  SRR4302535.484656/1‑94 (MQ=60)
                                              AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                                 >  SRR4302535.127624/1‑94 (MQ=60)
                                               AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                                                <  SRR4302535.2498747/94‑1 (MQ=60)
                                               AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                                                >  SRR4302535.3752236/1‑94 (MQ=60)
                                               AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                                                <  SRR4302535.2953472/94‑1 (MQ=60)
                                               AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                                                >  SRR4302535.1010775/1‑94 (MQ=60)
                                                ATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGC                                               >  SRR4302535.665207/1‑94 (MQ=60)
                                                 TCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCA                                              >  SRR4302535.4513380/1‑94 (MQ=60)
                                                  CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                                             >  SRR4302535.3574180/1‑94 (MQ=60)
                                                  CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                                             >  SRR4302535.4672/1‑94 (MQ=60)
                                                   ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                                            >  SRR4302535.3231476/1‑94 (MQ=60)
                                                   ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                                            >  SRR4302535.1427420/1‑94 (MQ=60)
                                                    CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                                           <  SRR4302535.468972/94‑1 (MQ=60)
                                                      CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAT                                         <  SRR4302535.4120446/94‑1 (MQ=60)
                                                      CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGCTCAGAAAACCGGCGCAAACAT                                         >  SRR4302535.797835/1‑94 (MQ=60)
                                                       GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        <  SRR4302535.3799531/94‑1 (MQ=60)
                                                       GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                                        <  SRR4302535.3554354/94‑1 (MQ=60)
                                                        CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                                       <  SRR4302535.678983/94‑1 (MQ=60)
                                                        CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                                       >  SRR4302535.4496021/1‑94 (MQ=60)
                                                        CATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTG                                       <  SRR4302535.683431/94‑1 (MQ=60)
                                                         ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      >  SRR4302535.2292638/1‑94 (MQ=60)
                                                         ACTGACGGCAAGGCGTAACGTCGCCTTAGCGACAACACTAATTTGCGAGTGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                                      <  SRR4302535.1753912/94‑1 (MQ=60)
                                                          TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     <  SRR4302535.3043677/94‑1 (MQ=60)
                                                          TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     >  SRR4302535.897621/1‑94 (MQ=60)
                                                          TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     <  SRR4302535.457062/94‑1 (MQ=60)
                                                          TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                                     <  SRR4302535.1002474/94‑1 (MQ=60)
                                                           TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                                    <  SRR4302535.677205/94‑1 (MQ=60)
                                                             ACGGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAC                                  >  SRR4302535.3789851/1‑94 (MQ=60)
                                                               GGCAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCG                                >  SRR4302535.1564262/1‑94 (MQ=60)
                                                                 CAAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCG                              >  SRR4302535.4511929/1‑94 (MQ=60)
                                                                  AAGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGC                             >  SRR4302535.872786/1‑94 (MQ=60)
                                                                   AGGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT                            >  SRR4302535.4390535/1‑94 (MQ=60)
                                                                    GGCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTC                           <  SRR4302535.2662413/94‑1 (MQ=60)
                                                                     GCGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCC                          <  SRR4302535.1785088/94‑1 (MQ=60)
                                                                      CGTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCA                         >  SRR4302535.546270/1‑94 (MQ=60)
                                                                       GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT                        >  SRR4302535.564801/1‑94 (MQ=60)
                                                                       GTAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT                        <  SRR4302535.2774927/94‑1 (MQ=60)
                                                                        TAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT                       >  SRR4302535.1428935/1‑94 (MQ=60)
                                                                        TAACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATT                       <  SRR4302535.2331139/94‑1 (MQ=60)
                                                                         AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG                      >  SRR4302535.1295777/1‑94 (MQ=60)
                                                                         AACGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTG                      >  SRR4302535.1049756/1‑94 (MQ=60)
                                                                           CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT                    <  SRR4302535.1031341/94‑1 (MQ=60)
                                                                           CGTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT                    >  SRR4302535.875138/1‑94 (MQ=60)
                                                                            GTCGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT                   >  SRR4302535.1582941/1‑94 (MQ=60)
                                                                              CGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCA                 >  SRR4302535.218829/1‑94 (MQ=60)
                                                                              CGCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCA                 >  SRR4302535.3114607/1‑94 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                >  SRR4302535.468929/1‑94 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302535.504503/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302535.177525/94‑1 (MQ=60)
                                                                               GCCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCAT                <  SRR4302535.4280233/94‑1 (MQ=60)
                                                                                CCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATT               >  SRR4302535.715606/1‑94 (MQ=60)
                                                                                CCGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATT               >  SRR4302535.2377255/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCGGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302535.2624921/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302535.4277446/1‑94 (MQ=60)
                                                                                 CGTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTG              >  SRR4302535.3008153/1‑94 (MQ=60)
                                                                                  GTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGG             >  SRR4302535.1590463/1‑94 (MQ=60)
                                                                                  GTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGG             <  SRR4302535.3490609/94‑1 (MQ=60)
                                                                                  GTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGG             >  SRR4302535.2608355/1‑94 (MQ=60)
                                                                                  GTAGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGG             <  SRR4302535.2615023/94‑1 (MQ=60)
                                                                                    AGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAG           <  SRR4302535.172550/94‑1 (MQ=60)
                                                                                    AGCGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAG           >  SRR4302535.276323/1‑94 (MQ=60)
                                                                                      CGACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAG         <  SRR4302535.2872415/94‑1 (MQ=60)
                                                                                       GACAACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGG        <  SRR4302535.688789/94‑1 (MQ=60)
                                                                                         CAACACGAATTGGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGC      <  SRR4302535.579497/94‑1 (MQ=60)
                                                                                          AACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCA     >  SRR4302535.556643/1‑94 (MQ=60)
                                                                                          AACACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCA     >  SRR4302535.3158214/1‑94 (MQ=60)
                                                                                            CACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATT   >  SRR4302535.1419251/1‑94 (MQ=60)
                                                                                            CACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATT   <  SRR4302535.2230012/94‑1 (MQ=60)
                                                                                             ACGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  <  SRR4302535.890731/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTTCATTGGAGAGGGCATTT  >  NC_000913/3961512‑3961698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑