Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,968,566 A→G L287L (TTA→TTG)  wecB → UDP‑N‑acetyl glucosamine ‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,5660AG100.0% 868.4 / NA 273L287L (TTA→TTG) wecBUDP‑N‑acetyl glucosamine ‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (159/114);  total (159/114)

Only 100 of 282 total aligned reads displayed.

GTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT  >  NC_000913/3968477‑3968655
                                                                                         |                                                                                         
gTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCg                                                                                        <  2:1782796/93‑1 (MQ=255)
gTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATACCCAGGAGTATTTGCCg                                                                                        >  1:2749828/1‑93 (MQ=255)
 tGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGt                                                                                       <  2:294429/93‑1 (MQ=255)
 tGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGt                                                                                       <  2:2790935/93‑1 (MQ=255)
   cATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGttt                                                                                     >  1:357424/1‑93 (MQ=255)
   cATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGttt                                                                                     >  2:3375674/1‑93 (MQ=255)
    aTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTg                                                                                    >  1:4474716/1‑93 (MQ=255)
    aTCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTg                                                                                    <  1:577575/93‑1 (MQ=255)
     tctcAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGt                                                                                   >  1:1855687/1‑93 (MQ=255)
        cAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTg                                                                                >  1:801647/1‑93 (MQ=255)
         aaCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTgg                                                                               >  1:2041516/1‑93 (MQ=255)
         aaCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTgg                                                                               >  1:1215704/1‑93 (MQ=255)
          aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              <  1:957238/93‑1 (MQ=255)
          aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              >  2:4408433/1‑93 (MQ=255)
          aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              >  2:234921/1‑93 (MQ=255)
          aCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGc                                                                              >  1:1470683/1‑93 (MQ=255)
           cccGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGCATTTGCCGTTTGTCTGGCt                                                                             >  2:2730975/1‑93 (MQ=255)
            ccGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtg                                                                            >  2:2269507/1‑93 (MQ=255)
             cGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtga                                                                           <  2:2686045/93‑1 (MQ=255)
                aCGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        >  2:3987673/1‑93 (MQ=255)
                aCGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        <  2:1633120/93‑1 (MQ=255)
                aCGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        >  2:1249528/1‑93 (MQ=255)
                aCGTCAGAGAACCGATCAATCGCATTCTGGGGAATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCtgatga                                                                        <  2:2894788/93‑1 (MQ=255)
                  gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      <  1:3851736/93‑1 (MQ=255)
                  gTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAc                                                                      >  2:1853364/1‑93 (MQ=255)
                   tCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAcc                                                                     >  1:3736544/1‑93 (MQ=255)
                    cAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCa                                                                    <  1:3416959/93‑1 (MQ=255)
                     agagAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAc                                                                   >  1:4270013/1‑93 (MQ=255)
                     agagAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAc                                                                   >  1:4449197/1‑93 (MQ=255)
                     agagAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAc                                                                   <  1:2118940/93‑1 (MQ=255)
                      gagaACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACg                                                                  >  1:3498340/1‑93 (MQ=255)
                        gaACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGcc                                                                <  2:4004442/93‑1 (MQ=255)
                         aaCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCt                                                               >  2:1479426/1‑93 (MQ=255)
                         aaCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCt                                                               <  1:1094672/93‑1 (MQ=255)
                          aCCGGTCAATCGCATTCTGGGGTATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTg                                                              <  2:3131976/93‑1 (MQ=255)
                          aCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTg                                                              >  1:1079855/1‑93 (MQ=255)
                          aCCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTg                                                              >  1:2838050/1‑93 (MQ=255)
                           ccGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTgg                                                             <  1:878765/93‑1 (MQ=255)
                              gTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTg                                                          >  1:4187965/1‑93 (MQ=255)
                              gTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTg                                                          <  1:2329677/93‑1 (MQ=255)
                                cAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAt                                                        <  1:708262/93‑1 (MQ=255)
                                cAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAt                                                        <  1:333999/93‑1 (MQ=255)
                                 aaTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtt                                                       >  1:2983999/1‑93 (MQ=255)
                                 aaTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtt                                                       >  1:1477149/1‑93 (MQ=255)
                                  aTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAttt                                                      <  1:2883312/93‑1 (MQ=255)
                                  aTCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAttt                                                      <  1:133364/93‑1 (MQ=255)
                                   tCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtttt                                                     >  1:2198371/1‑93 (MQ=255)
                                   gcGCAATCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTGGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGAtttt                                                     <  2:3867384/92‑1 (MQ=255)
                                       aTTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAcc                                                 >  2:2060212/1‑93 (MQ=255)
                                       aTTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAcc                                                 <  1:1949423/93‑1 (MQ=255)
                                       aTTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAcc                                                 <  2:824531/93‑1 (MQ=255)
                                        ttCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCg                                                <  2:4031708/93‑1 (MQ=255)
                                             gggCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCa                                           >  2:849691/1‑93 (MQ=255)
                                             gggCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCa                                           <  2:2012614/93‑1 (MQ=255)
                                             gggCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCa                                           <  1:1724270/93‑1 (MQ=255)
                                             gggCATGTAAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCa                                           <  2:4483571/93‑1 (MQ=255)
                                               gCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAgg                                         >  2:4198942/1‑93 (MQ=255)
                                                cATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAggc                                        <  1:2593207/93‑1 (MQ=255)
                                                  tgtgAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAggcgg                                      >  2:3151480/1‑93 (MQ=255)
                                                    tgAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGTCGGCa                                    >  2:4522919/1‑93 (MQ=255)
                                                       aaaaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTc                                 <  2:3581087/93‑1 (MQ=255)
                                                       aaaaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGATTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTc                                 >  2:4632308/1‑93 (MQ=255)
                                                         aaTGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAg                               <  1:3978322/93‑1 (MQ=255)
                                                           tGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGga                             <  2:2165675/93‑1 (MQ=255)
                                                            gTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaa                            >  1:4609631/1‑93 (MQ=255)
                                                            gTCATTCTGATCGATCCCCAGGAGGATTTGCCGTTTGGCTGGCTGATGAACCACGCCTGGCTGATTTTGACTGACTCAGGCGGCATTCAgtca                            >  1:1627946/1‑90 (MQ=255)
                                                            gTCATTCTGATCGATACCCAGGAGTATTTGCCGTTTGTCGGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaa                            <  1:64973/93‑1 (MQ=255)
                                                             tCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaag                           >  1:4636904/1‑93 (MQ=255)
                                                             tCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaag                           <  2:1168841/93‑1 (MQ=255)
                                                              cATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaaga                          >  1:1796092/1‑93 (MQ=255)
                                                               aTTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaagaa                         <  1:544721/93‑1 (MQ=255)
                                                               aTTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGgaagaa                         <  2:1116570/93‑1 (MQ=255)
                                                                ttCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGaagaag                        >  1:1580223/1‑93 (MQ=255)
                                                                ttCTGATCGATCCCCAGAAGTATTTGCCGTTTGTCTGGCTGATGAACCGCGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGaagaag                        <  2:3192397/93‑1 (MQ=255)
                                                                 tCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAgc                       <  2:2423089/93‑1 (MQ=255)
                                                                    gatcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGcc                    >  2:2551970/1‑93 (MQ=255)
                                                                    gatcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGcc                    <  2:466958/93‑1 (MQ=255)
                                                                    gaacgaccCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGcc                    <  2:321781/90‑1 (MQ=255)
                                                                     atcgatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCt                   <  1:3395983/93‑1 (MQ=255)
                                                                        gatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCg                >  2:3783054/1‑93 (MQ=255)
                                                                        gatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCg                >  1:1832303/1‑93 (MQ=255)
                                                                        gatcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCg                >  2:3505202/1‑93 (MQ=255)
                                                                         atcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGc               >  1:3162777/1‑93 (MQ=255)
                                                                         atcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCCCCTTCGc               <  2:3339848/93‑1 (MQ=255)
                                                                         atcCCCAGGAGTATTTGCCGTGTGTCTTGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGc               <  1:2653712/93‑1 (MQ=255)
                                                                          tcCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCt              >  2:4093246/1‑93 (MQ=255)
                                                                           ccccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTg             <  2:4149453/93‑1 (MQ=255)
                                                                           ccccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTg             <  2:647362/93‑1 (MQ=255)
                                                                            cccAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTgg            <  2:545127/93‑1 (MQ=255)
                                                                              cAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTgggg          >  2:2093949/1‑93 (MQ=255)
                                                                               aGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGa         >  1:1998232/1‑93 (MQ=255)
                                                                                ggAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaa        >  2:2961834/1‑93 (MQ=255)
                                                                                ggAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaa        >  2:1085080/1‑93 (MQ=255)
                                                                                ggAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaa        <  1:1293376/93‑1 (MQ=255)
                                                                                 gattATTTGGCGTTTGTCTGGCTGATGAACCACGACTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaaa       <  1:386842/90‑1 (MQ=255)
                                                                                 gAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGaaa       >  1:2820970/1‑93 (MQ=255)
                                                                                  aGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAAc      <  2:2588857/93‑1 (MQ=255)
                                                                                     aTTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCtg   >  2:4312026/1‑93 (MQ=255)
                                                                                      tttGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCtgt  >  2:3712922/1‑93 (MQ=255)
                                                                                      tttGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCtgt  <  2:3311441/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT  >  NC_000913/3968477‑3968655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTG  >  NC_000913/3968476‑3968659
                                                                                          |                                                                                             
GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCG                                                                                            >  SRR4302535.1738127/1‑94 (MQ=60)
GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATACCCAGGAGTATTTGCCG                                                                                            >  SRR4302535.2769432/1‑94 (MQ=60)
   GCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTT                                                                                         >  SRR4302535.359895/1‑94 (MQ=60)
    CATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTG                                                                                        >  SRR4302535.4505418/1‑94 (MQ=60)
     ATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGT                                                                                       >  SRR4302535.1869055/1‑94 (MQ=60)
     ATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGT                                                                                       <  SRR4302535.581631/94‑1 (MQ=60)
       CTCAGCCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCT                                                                                     <  SRR4302535.1112383/94‑1 (MQ=60)
       CTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCT                                                                                     <  SRR4302535.2975791/94‑1 (MQ=60)
       CTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCT                                                                                     >  SRR4302535.427673/1‑94 (MQ=60)
        TCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTG                                                                                    >  SRR4302535.807279/1‑94 (MQ=60)
        TCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTG                                                                                    <  SRR4302535.1576751/94‑1 (MQ=60)
         CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                   <  SRR4302535.1302985/94‑1 (MQ=60)
         CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                   >  SRR4302535.2056221/1‑94 (MQ=60)
         CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                   <  SRR4302535.223629/94‑1 (MQ=60)
         CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                   >  SRR4302535.1224424/1‑94 (MQ=60)
         CAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGG                                                                                   <  SRR4302535.1286387/94‑1 (MQ=60)
          AACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGC                                                                                  >  SRR4302535.1481216/1‑94 (MQ=60)
           ACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCT                                                                                 <  SRR4302535.964033/94‑1 (MQ=60)
           ACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCT                                                                                 <  SRR4302535.355383/94‑1 (MQ=60)
              CGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGAT                                                                              >  SRR4302535.3100606/1‑94 (MQ=60)
               GAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATG                                                                             <  SRR4302535.3945806/94‑1 (MQ=60)
               GAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATG                                                                             >  SRR4302535.761839/1‑94 (MQ=60)
               GAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATG                                                                             <  SRR4302535.2006154/94‑1 (MQ=60)
               GAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATG                                                                             >  SRR4302535.1751366/1‑94 (MQ=60)
                  CGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAC                                                                          <  SRR4302535.3415891/94‑1 (MQ=60)
                  CGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAAC                                                                          >  SRR4302535.550496/1‑94 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACC                                                                         >  SRR4302535.3762405/1‑94 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACC                                                                         <  SRR4302535.90993/94‑1 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACC                                                                         >  SRR4302535.3553126/1‑94 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACC                                                                         <  SRR4302535.3878239/94‑1 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACC                                                                         <  SRR4302535.3878240/94‑1 (MQ=60)
                   GTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACA                                                                         >  SRR4302535.3856832/1‑94 (MQ=60)
                    TCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCCGGAGTATTTGCCGTTTGTCTGGCTGATGAACCA                                                                        >  SRR4302535.1822971/1‑94 (MQ=60)
                     CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                       >  SRR4302535.4479714/1‑94 (MQ=60)
                     CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                       >  SRR4302535.4299247/1‑94 (MQ=60)
                     CAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCAC                                                                       <  SRR4302535.3440729/94‑1 (MQ=60)
                      AGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACG                                                                      <  SRR4302535.2134224/94‑1 (MQ=60)
                      AGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACG                                                                      >  SRR4302535.3522653/1‑94 (MQ=60)
                      AGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACG                                                                      <  SRR4302535.3420349/94‑1 (MQ=60)
                         GAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCT                                                                   >  SRR4302535.2114061/1‑94 (MQ=60)
                          AACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTG                                                                  >  SRR4302535.2858185/1‑94 (MQ=60)
                          AACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTG                                                                  >  SRR4302535.1087539/1‑94 (MQ=60)
                          AACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTG                                                                  <  SRR4302535.4178516/94‑1 (MQ=60)
                          AACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTG                                                                  <  SRR4302535.1102462/94‑1 (MQ=60)
                           ACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGG                                                                 <  SRR4302535.730057/94‑1 (MQ=60)
                            CCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGC                                                                >  SRR4302535.3963725/1‑94 (MQ=60)
                            CCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGC                                                                <  SRR4302535.884986/94‑1 (MQ=60)
                              GGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTG                                                              >  SRR4302535.4216628/1‑94 (MQ=60)
                              GGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTG                                                              <  SRR4302535.1618429/94‑1 (MQ=60)
                               GTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGA                                                             <  SRR4302535.2346339/94‑1 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           >  SRR4302535.3004930/1‑94 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           <  SRR4302535.713233/94‑1 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           >  SRR4302535.1487724/1‑94 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           >  SRR4302535.4546555/1‑94 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           <  SRR4302535.336310/94‑1 (MQ=60)
                                 CAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATT                                                           >  SRR4302535.878446/1‑94 (MQ=60)
                                  AATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGAGCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTT                                                          <  SRR4302535.4566683/94‑1 (MQ=60)
                                   ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                         <  SRR4302535.134298/94‑1 (MQ=60)
                                   ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                         <  SRR4302535.2903717/94‑1 (MQ=60)
                                   ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                         >  SRR4302535.265067/1‑94 (MQ=60)
                                   ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                         >  SRR4302535.2149543/1‑94 (MQ=60)
                                   ATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTT                                                         >  SRR4302535.2214035/1‑94 (MQ=60)
                                      GCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAC                                                      <  SRR4302535.588068/94‑1 (MQ=60)
                                      GCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGAC                                                      >  SRR4302535.579842/1‑94 (MQ=60)
                                       CATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACC                                                     >  SRR4302535.1482845/1‑94 (MQ=60)
                                        ATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCG                                                    >  SRR4302535.2851065/1‑94 (MQ=60)
                                        ATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCG                                                    <  SRR4302535.1963455/94‑1 (MQ=60)
                                        ATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCG                                                    >  SRR4302535.2770042/1‑94 (MQ=60)
                                           CTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACT                                                 <  SRR4302535.1101524/94‑1 (MQ=60)
                                              GGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAG                                              >  SRR4302535.469306/1‑94 (MQ=60)
                                              GGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAG                                              <  SRR4302535.1736743/94‑1 (MQ=60)
                                                GCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGC                                            >  SRR4302535.3191743/1‑94 (MQ=60)
                                                 CATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCG                                           <  SRR4302535.2611836/94‑1 (MQ=60)
                                                  ATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGG                                          >  SRR4302535.4642168/1‑94 (MQ=60)
                                                   TGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGC                                         >  SRR4302535.3611993/1‑94 (MQ=60)
                                                     TGAAAAATGTCATTCTGATCGATCCCCAGGAGGATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCCT                                       >  SRR4302535.593513/1‑94 (MQ=60)
                                                      GAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATT                                      >  SRR4302535.1383213/1‑94 (MQ=60)
                                                       AAAAATGTCATTCAGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTC                                     <  SRR4302535.2696273/94‑1 (MQ=60)
                                                        AAAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCA                                    <  SRR4302535.906449/94‑1 (MQ=60)
                                                         AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                   >  SRR4302535.321811/1‑94 (MQ=60)
                                                         AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                   <  SRR4302535.3795163/94‑1 (MQ=60)
                                                         AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                   >  SRR4302535.3167643/1‑94 (MQ=60)
                                                         AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                   <  SRR4302535.696498/94‑1 (MQ=60)
                                                         AAATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAG                                   <  SRR4302535.1376545/94‑1 (MQ=60)
                                                          AATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGG                                  <  SRR4302535.4005507/94‑1 (MQ=60)
                                                           ATGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGA                                 >  SRR4302535.3641052/1‑94 (MQ=60)
                                                            TGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAA                                >  SRR4302535.85923/1‑94 (MQ=60)
                                                            TGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAA                                >  SRR4302535.4640953/1‑94 (MQ=60)
                                                            TGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAA                                >  SRR4302535.1967683/1‑94 (MQ=60)
                                                            TGTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAA                                >  SRR4302535.1924356/1‑94 (MQ=60)
                                                            TGTCATTCTGATCGATCCCCAGGAGGATTTGCCGTTTGGCTGGCTGATGAACCACGCCTGGCTGATTTTGACTGACTCAGGCGGCATTCAGtca                                >  SRR4302535.1639698/1‑91 (MQ=60)
                                                             GTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAG                               >  SRR4302535.4668357/1‑94 (MQ=60)
                                                             GTCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAG                               >  SRR4302535.1707300/1‑94 (MQ=60)
                                                             GTCATTCTGATCGATACCCAGGAGTATTTGCCGTTTGTCGGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAG                               <  SRR4302535.65463/94‑1 (MQ=60)
                                                              TCATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGA                              >  SRR4302535.1809077/1‑94 (MQ=60)
                                                               CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                             >  SRR4302535.1295872/1‑94 (MQ=60)
                                                               CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                             >  SRR4302535.2254119/1‑94 (MQ=60)
                                                               CATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAA                             <  SRR4302535.160649/94‑1 (MQ=60)
                                                                ATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAG                            <  SRR4302535.548527/94‑1 (MQ=60)
                                                                ATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAG                            <  SRR4302535.2029458/94‑1 (MQ=60)
                                                                ATTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAG                            >  SRR4302535.1591630/1‑94 (MQ=60)
                                                                 TTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGC                           >  SRR4302535.2603061/1‑94 (MQ=60)
                                                                 TTCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGC                           <  SRR4302535.2125365/94‑1 (MQ=60)
                                                                  TCTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCG                          <  SRR4302535.1819223/94‑1 (MQ=60)
                                                                   CTGATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGC                         >  SRR4302535.193505/1‑94 (MQ=60)
                                                                      ATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTT                      <  SRR4302535.3419608/94‑1 (MQ=60)
                                                                      ATCGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTT                      <  SRR4302535.3424567/94‑1 (MQ=60)
                                                                        CGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCG                    <  SRR4302535.4289283/94‑1 (MQ=60)
                                                                        CGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCG                    >  SRR4302535.1845524/1‑94 (MQ=60)
                                                                        CGATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCG                    >  SRR4302535.3513707/1‑94 (MQ=60)
                                                                         GATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGC                   >  SRR4302535.3184713/1‑94 (MQ=60)
                                                                          ATCCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCT                  <  SRR4302535.1514034/94‑1 (MQ=60)
                                                                          ATCCCCAGGAGTATTTGCCGTGTGTCTTGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCT                  <  SRR4302535.2672723/94‑1 (MQ=60)
                                                                           TTCCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTG                 <  SRR4302535.3519309/94‑1 (MQ=60)
                                                                           TCCCCAGGAGAATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGGCTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTG                 >  SRR4302535.2773620/1‑94 (MQ=60)
                                                                             CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG               <  SRR4302535.2977250/94‑1 (MQ=60)
                                                                             CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG               >  SRR4302535.449135/1‑94 (MQ=60)
                                                                             CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG               <  SRR4302535.4183189/94‑1 (MQ=60)
                                                                             CCCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG               >  SRR4302535.2048227/1‑94 (MQ=60)
                                                                             CCCAGGAGTATTTGCCGTGTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGG               <  SRR4302535.497623/94‑1 (MQ=60)
                                                                              CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG              >  SRR4302535.2614481/1‑94 (MQ=60)
                                                                              CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG              >  SRR4302535.2454398/1‑94 (MQ=60)
                                                                              CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG              >  SRR4302535.2681131/1‑94 (MQ=60)
                                                                              CCAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGG              >  SRR4302535.1334590/1‑94 (MQ=60)
                                                                               CAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGA             >  SRR4302535.2012625/1‑94 (MQ=60)
                                                                               CAGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGA             >  SRR4302535.4403081/1‑94 (MQ=60)
                                                                                AGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAA            >  SRR4302535.1713491/1‑94 (MQ=60)
                                                                                AGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAA            >  SRR4302535.438292/1‑94 (MQ=60)
                                                                                AGGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAA            >  SRR4302535.2888756/1‑94 (MQ=60)
                                                                                 GGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAA           <  SRR4302535.1302592/94‑1 (MQ=60)
                                                                                 GGAGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAA           >  SRR4302535.2841005/1‑94 (MQ=60)
                                                                                   AGTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACC         >  SRR4302535.186570/1‑94 (MQ=60)
                                                                                    GTATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCT        >  SRR4302535.2865137/1‑94 (MQ=60)
                                                                                     TATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTG       >  SRR4302535.4660247/1‑94 (MQ=60)
                                                                                      ATTTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGT      <  SRR4302535.1145608/94‑1 (MQ=60)
                                                                                        TTGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGC    >  SRR4302535.725192/1‑94 (MQ=60)
                                                                                         TGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT   >  SRR4302535.865862/1‑94 (MQ=60)
                                                                                         TGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT   >  SRR4302535.2213468/1‑94 (MQ=60)
                                                                                         TGCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCT   >  SRR4302535.2305814/1‑94 (MQ=60)
                                                                                          GCCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTG  <  SRR4302535.1872245/94‑1 (MQ=60)
                                                                                          GCCGTATGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTG  >  SRR4302535.2705621/1‑94 (MQ=60)
                                                                                          |                                                                                             
GGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCCTGGCTGATTTTGACCGACTCAGGCGGCATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTG  >  NC_000913/3968476‑3968659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑