Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG99.6% 739.1 / ‑5.3 238R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (112/125);  minor base A (1/0);  total (113/125)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*NC_0009133,969,1040GC100.0% 726.5 / NA 231R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (108/123);  total (108/123)

Only 100 of 242 total aligned reads displayed.

TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969017‑3969188
                                                                                      ||                                                                                    
tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                 >  2:2941892/1‑93 (MQ=255)
tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                 <  1:2894082/93‑1 (MQ=255)
  gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg                                                                               >  2:1451715/1‑93 (MQ=255)
   tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGGTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                              >  2:393655/1‑93 (MQ=255)
     aacccTGTTTGGGTGAGTGAAAAAAAACCTGCCGTAGAAGGCGTTTTTTTACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                            <  2:189413/90‑1 (MQ=255)
      aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                           >  2:4294791/1‑93 (MQ=255)
       acttGATTGGGCGAGTGTAGAAAAACCGGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                          <  2:214478/90‑1 (MQ=255)
       aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                          >  1:1893904/1‑93 (MQ=255)
       aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                          >  1:4060400/1‑93 (MQ=255)
        ccTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt                                                                         >  1:3362115/1‑93 (MQ=255)
        ccTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt                                                                         <  2:4504988/93‑1 (MQ=255)
         cTGATTGGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                        <  2:1808096/93‑1 (MQ=255)
          tgttttGGCGAGTGTAGAAAAACCTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                       <  1:3329780/90‑1 (MQ=255)
           gttttGGCGAGTTAAAAAAAACCTCCCGAAGAAGGCGGTTTTTTACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa                                                                      <  2:2557636/91‑1 (MQ=255)
            aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                     >  1:4516825/1‑93 (MQ=255)
             tttGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                    <  2:4685912/93‑1 (MQ=255)
               tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                  <  1:1377198/93‑1 (MQ=255)
                 gCGAGTGTAGTAAAAACTGCCGTAGGAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGGCTGGCTGATTGGTGTACCCaag                                                                >  2:3301520/1‑91 (MQ=255)
                   gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                              <  1:1232616/93‑1 (MQ=255)
                    aGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg                                                             >  2:3530324/1‑93 (MQ=255)
                      tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                           >  2:2760616/1‑93 (MQ=255)
                       gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                          <  1:3862550/93‑1 (MQ=255)
                         agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                        <  1:4416274/93‑1 (MQ=255)
                         agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                        <  1:4380824/93‑1 (MQ=255)
                          gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  2:2899073/93‑1 (MQ=255)
                          gtaAAAACGGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  2:3440263/93‑1 (MQ=255)
                          ataAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  2:2009174/92‑1 (MQ=255)
                          ataAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       <  1:3513995/92‑1 (MQ=255)
                            aaaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                     <  1:3584098/93‑1 (MQ=255)
                             aaacCTGCGGTAGAGGGTGTTTTTTTCGGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                    <  1:4002700/93‑1 (MQ=255)
                               aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                  >  1:2162670/1‑93 (MQ=255)
                               aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                  >  1:446254/1‑93 (MQ=255)
                                acggCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                 <  1:2576047/90‑1 (MQ=255)
                                   gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              >  1:2416547/1‑93 (MQ=255)
                                   gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              >  1:1968671/1‑93 (MQ=255)
                                    ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                             <  1:1478857/93‑1 (MQ=255)
                                     cGTAGAAGGCGGCTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                            >  1:1729023/1‑93 (MQ=255)
                                      gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                           >  2:2851122/1‑93 (MQ=255)
                                      gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                           >  2:413213/1‑93 (MQ=255)
                                       tAGAAGGCGGTTTTTTACGAGCGAGAACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCAGTACCCACGCCGTTTAAGGGCGATCATGGGc                                          >  2:704580/1‑93 (MQ=255)
                                        aGAAGGCGGCTTTTCACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                         <  2:3642566/93‑1 (MQ=255)
                                         gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                        >  1:2233282/1‑93 (MQ=255)
                                           aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      <  2:2628666/93‑1 (MQ=255)
                                           aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      >  2:4257906/1‑93 (MQ=255)
                                            ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                     <  1:2024982/93‑1 (MQ=255)
                                             gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                    <  2:811536/93‑1 (MQ=255)
                                              gggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat                                   <  1:4623466/92‑1 (MQ=255)
                                               ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                  <  2:1555462/93‑1 (MQ=255)
                                               ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAAGGCGATCATGAGGCAGctaag                                  >  2:2433773/1‑91 (MQ=255)
                                                gTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATgc                                 >  1:3917708/1‑92 (MQ=255)
                                                 ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                <  1:667040/93‑1 (MQ=255)
                                                  tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                               <  1:3004111/93‑1 (MQ=255)
                                                  tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATAAGCCAGATATGAcc                               <  1:505507/93‑1 (MQ=255)
                                                    tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             <  1:3028259/93‑1 (MQ=255)
                                                    tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             <  1:3459731/93‑1 (MQ=255)
                                                    tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGGTTAAGGGCGATCATGAGCCAGATATGACCtc                             >  2:2667041/1‑92 (MQ=255)
                                                     tgaCGAGCTAGCACGACGCCAGTTGAAGCTGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                            <  2:2190076/91‑1 (MQ=255)
                                                     taaCGAGCGAGCACGACGCCAGTTGAAGCGGATGCGTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                            <  2:3223078/91‑1 (MQ=255)
                                                       aCGAGCGCGGACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                          <  2:1816652/93‑1 (MQ=255)
                                                          agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa                       >  1:1127483/1‑93 (MQ=255)
                                                           gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                      >  1:4236253/1‑93 (MQ=255)
                                                            cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                     <  2:481534/93‑1 (MQ=255)
                                                             gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                    >  1:1226632/1‑93 (MQ=255)
                                                             gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                    <  2:1768422/93‑1 (MQ=255)
                                                               gcgcgacgCCACTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGACCCAGATATGACCTACGTTGAATCgg                  <  2:2049011/90‑1 (MQ=255)
                                                               gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  <  1:4303532/93‑1 (MQ=255)
                                                               gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  >  2:3612309/1‑93 (MQ=255)
                                                               gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  >  2:1297393/1‑93 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:1851695/93‑1 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  1:265981/93‑1 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  1:3721098/93‑1 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:2074132/93‑1 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:1250595/93‑1 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                >  2:318746/1‑93 (MQ=255)
                                                                  cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg               >  1:2537243/1‑93 (MQ=255)
                                                                  cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg               >  1:721880/1‑93 (MQ=255)
                                                                    acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             >  1:1578177/1‑93 (MQ=255)
                                                                    acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             >  2:3041234/1‑93 (MQ=255)
                                                                     cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc            <  1:1445856/93‑1 (MQ=255)
                                                                     cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc            >  2:1907753/1‑93 (MQ=255)
                                                                       ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc          <  2:1369256/93‑1 (MQ=255)
                                                                       ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc          >  1:3289424/1‑93 (MQ=255)
                                                                        cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgct         <  2:3852931/93‑1 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc        <  1:3977588/93‑1 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc        >  2:763402/1‑93 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc        <  1:1684307/93‑1 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc        <  2:3372602/93‑1 (MQ=255)
                                                                          gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc       >  2:2016917/1‑93 (MQ=255)
                                                                          gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc       <  1:1312205/93‑1 (MQ=255)
                                                                          gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc       >  2:627381/1‑93 (MQ=255)
                                                                          gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTAAATCGGCTGCTCGCTcc       >  2:2197580/1‑93 (MQ=255)
                                                                           ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCa      >  2:1694345/1‑93 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTCCCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt     <  2:2376451/93‑1 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAGTCGGCTGCTCGCTCCAt     <  2:3959336/93‑1 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt     <  2:4442975/93‑1 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt     >  2:4609568/1‑93 (MQ=255)
                                                                             gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    <  1:2841547/93‑1 (MQ=255)
                                                                             gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    >  1:983179/1‑93 (MQ=255)
                                                                              aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg   >  1:2301257/1‑93 (MQ=255)
                                                                               aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc  >  1:906611/1‑93 (MQ=255)
                                                                                      ||                                                                                    
TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969017‑3969188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192
                                                                                             |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302535.2797558/1‑94 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302535.1944662/1‑94 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      <  SRR4302535.288627/94‑1 (MQ=60)
      accgtaaacctTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGTGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     <  SRR4302535.101231/83‑1 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     >  SRR4302535.416686/1‑94 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                    <  SRR4302535.2914552/94‑1 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                    <  SRR4302535.4174617/94‑1 (MQ=60)
        CGTCGACCCTGATTTGGCGAGTAAAGAAAAACCTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                   <  SRR4302535.4057568/94‑1 (MQ=60)
        CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                   >  SRR4302535.4008103/1‑94 (MQ=60)
         GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                                  >  SRR4302535.3031066/1‑94 (MQ=60)
          TCGAGCCTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302535.4424765/94‑1 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                >  SRR4302535.2641103/1‑94 (MQ=60)
            aacccggtttgggcgagtaaagaaaaacCTGCCGAAGAGGGCGTTTTTTAACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302535.2458555/66‑1 (MQ=36)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302535.1266815/94‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302535.2441766/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302535.1907539/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302535.4088192/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302535.1476177/1‑94 (MQ=60)
              ACTTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302535.94425/94‑1 (MQ=60)
              ACCTGATTTGGCGCGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302535.2695053/94‑1 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             >  SRR4302535.3385512/1‑94 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             >  SRR4302535.2029063/1‑94 (MQ=60)
                 TGTTTTGGCGAGTGTAGAAAAACCTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          <  SRR4302535.3352949/94‑1 (MQ=60)
                 TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          >  SRR4302535.1396164/1‑94 (MQ=60)
                 TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          >  SRR4302535.747125/1‑94 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302535.4547787/1‑94 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302535.3218620/1‑94 (MQ=60)
                    TTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                       <  SRR4302535.2567546/94‑1 (MQ=60)
                    TTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                       <  SRR4302535.3019823/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302535.1387027/94‑1 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302535.1298024/1‑94 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 >  SRR4302535.2872106/1‑94 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 <  SRR4302535.1241433/94‑1 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 >  SRR4302535.3401031/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                >  SRR4302535.1514742/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                >  SRR4302535.184617/1‑94 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302535.2141915/1‑94 (MQ=60)
                             ggtgtaaaaaacggcggtagagggggttttttgaggggcggGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302535.4161/53‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302535.3908785/1‑94 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302535.47000/1‑94 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             <  SRR4302535.3889125/94‑1 (MQ=60)
                               TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                            <  SRR4302535.2731210/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302535.4446503/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302535.4410797/94‑1 (MQ=60)
                                 ATAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302535.3538414/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        >  SRR4302535.2393830/1‑94 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302535.3608956/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATACCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGG                                                        >  SRR4302535.432642/1‑94 (MQ=60)
                                    aaacctgcggtagagggtgtttttttcgGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302535.4030079/66‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       >  SRR4302535.1739445/1‑94 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302535.1151298/94‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302535.1391999/94‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       >  SRR4302535.3463938/1‑94 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      >  SRR4302535.449376/1‑94 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      >  SRR4302535.2178187/1‑94 (MQ=60)
                                       ACGGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    <  SRR4302535.2594561/94‑1 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  >  SRR4302535.2433943/1‑94 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  >  SRR4302535.1982856/1‑94 (MQ=60)
                                           CCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302535.1489440/94‑1 (MQ=60)
                                           CCGTAGAAGGCGGCTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                >  SRR4302535.1741518/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302535.4565860/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302535.2249166/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          >  SRR4302535.2916382/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          >  SRR4302535.584163/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302535.4504891/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        <  SRR4302535.2039575/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302535.3590131/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302535.4095599/94‑1 (MQ=60)
                                                     GGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302535.4654867/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGC                                     >  SRR4302535.3944511/1‑94 (MQ=60)
                                                        TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302535.3858097/94‑1 (MQ=60)
                                                        TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302535.671691/94‑1 (MQ=60)
                                                        TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   >  SRR4302535.2295285/1‑94 (MQ=60)
                                                        TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGGTTAAGGGCGCTCATGAGCCAGAcaccgac                                   >  SRR4302535.2295286/1‑87 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  >  SRR4302535.226269/1‑94 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302535.3025168/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATAAGCCAGATATGACCT                                  <  SRR4302535.509040/94‑1 (MQ=60)
                                                          TTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                 <  SRR4302535.3530487/94‑1 (MQ=60)
                                                          TTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                 <  SRR4302535.809779/94‑1 (MQ=60)
                                                           TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302535.3589073/94‑1 (MQ=60)
                                                           TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302535.3483785/94‑1 (MQ=60)
                                                           TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302535.3049458/94‑1 (MQ=60)
                                                           TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302535.3983600/94‑1 (MQ=60)
                                                              ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302535.1593271/94‑1 (MQ=60)
                                                               CGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            <  SRR4302535.2015666/94‑1 (MQ=60)
                                                               CGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGGTG                            >  SRR4302535.3318541/1‑94 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           >  SRR4302535.1135534/1‑94 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           >  SRR4302535.1360675/1‑94 (MQ=60)
                                                                 AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          >  SRR4302535.3848806/1‑94 (MQ=60)
                                                                 AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          >  SRR4302535.4265246/1‑94 (MQ=60)
                                                                  GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302535.2182087/94‑1 (MQ=60)
                                                                  GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302535.4413161/94‑1 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        >  SRR4302535.1235406/1‑94 (MQ=60)
                                                                    GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302535.1931546/94‑1 (MQ=60)
                                                                      GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     <  SRR4302535.4009123/94‑1 (MQ=60)
                                                                      GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     <  SRR4302535.1845519/94‑1 (MQ=60)
                                                                      GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     <  SRR4302535.4332975/94‑1 (MQ=60)
                                                                      GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     >  SRR4302535.1240453/1‑94 (MQ=60)
                                                                       CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302535.3746877/94‑1 (MQ=60)
                                                                       CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    >  SRR4302535.3382920/1‑94 (MQ=60)
                                                                       CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302535.267829/94‑1 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302535.2555521/1‑94 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302535.726946/1‑94 (MQ=60)
                                                                          GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302535.1589567/1‑94 (MQ=60)
                                                                          GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302535.324360/1‑94 (MQ=60)
                                                                            CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302535.1456195/94‑1 (MQ=60)
                                                                             GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              >  SRR4302535.3312295/1‑94 (MQ=60)
                                                                                AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302535.4483140/1‑94 (MQ=60)
                                                                                AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           <  SRR4302535.1696505/94‑1 (MQ=60)
                                                                                AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           <  SRR4302535.4004762/94‑1 (MQ=60)
                                                                                 GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302535.1321584/94‑1 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302535.1682556/94‑1 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        >  SRR4302535.254746/1‑94 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        >  SRR4302535.990163/1‑94 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302535.4545511/94‑1 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302535.2317728/1‑94 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302535.2861700/94‑1 (MQ=60)
                                                                                     AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302535.4668024/94‑1 (MQ=60)
                                                                                     AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      >  SRR4302535.913046/1‑94 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302535.4359186/94‑1 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302535.720735/94‑1 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     >  SRR4302535.1167644/1‑94 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCC     >  SRR4302535.36629/1‑94 (MQ=60)
                                                                                         GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302535.1177165/1‑94 (MQ=60)
                                                                                             |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑