Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,692	T→C	C187R (TGC→CGC)	rffG →	dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,692	0	T	C	95.9%	566.0 / 8.7	195	C187R (TGC→CGC)	rffG	dTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (110/77); total (116/79)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 230 total aligned reads displayed.

AGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCTGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTA  >  NC_000913/3970602‑3970781

 gcagcCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCt                                                                                        <  2:1003571/93‑1 (MQ=255)

  cagcCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTc                                                                                       <  1:3403010/93‑1 (MQ=255)

   agcCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCg                                                                                      >  2:63377/1‑93 (MQ=255)

      ccTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAAt                                                                                   >  1:462673/1‑93 (MQ=255)

      ccTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAAt                                                                                   >  2:2266094/1‑93 (MQ=255)

       cTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATa                                                                                  >  1:18365/1‑93 (MQ=255)

        ttATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATaa                                                                                 >  1:1123680/1‑93 (MQ=255)

         tATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAc                                                                                <  2:4165399/93‑1 (MQ=255)

         tATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAc                                                                                >  1:3451457/1‑93 (MQ=255)

          aTTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACt                                                                               >  1:2342585/1‑93 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCATCAACCGCTCGAATAACTa                                                                              <  1:2614031/93‑1 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTa                                                                              <  1:1018285/93‑1 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTa                                                                              >  2:3896164/1‑93 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTa                                                                              <  2:427044/93‑1 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTa                                                                              <  1:4418874/93‑1 (MQ=255)

           ttCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTa                                                                              >  2:1225791/1‑93 (MQ=255)

            tCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTAc                                                                             <  1:1525504/93‑1 (MQ=255)

             ccGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACg                                                                            >  2:456709/1‑93 (MQ=255)

              cgcgTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACgg                                                                           >  2:2589018/1‑93 (MQ=255)

                cgTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGcc                                                                         >  1:330247/1‑93 (MQ=255)

                 gTCAAAAGCCAGCAGCGACCATCTGGTGTGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGccc                                                                        <  1:658593/93‑1 (MQ=255)

                 gTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGccc                                                                        >  2:3041767/1‑93 (MQ=255)

                  tCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCt                                                                       <  1:2905777/93‑1 (MQ=255)

                  tCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCt                                                                       >  1:693763/1‑93 (MQ=255)

                   cAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTGTCACCAACCGCTCGAATAACTACGGCCCtt                                                                      <  1:2420032/93‑1 (MQ=255)

                    aaaaGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTa                                                                     >  2:2494274/1‑93 (MQ=255)

                    aaaaGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTa                                                                     >  1:3253696/1‑93 (MQ=255)

                    aaaaGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTa                                                                     >  2:4641603/1‑93 (MQ=255)

                       aGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCa                                                                  >  2:1673938/1‑93 (MQ=255)

                         ccAGCAGCGCCCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTGTCACCAGCCGCTCGAATAACTACGGCCCTTACctct                                                                <  2:2313145/93‑1 (MQ=255)

                           agcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACttt                                                              >  1:433584/1‑93 (MQ=255)

                           agcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACttt                                                              >  2:742970/1‑93 (MQ=255)

                            gcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTc                                                             <  2:3095632/93‑1 (MQ=255)

                            gcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTc                                                             >  1:1143947/1‑93 (MQ=255)

                            gcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTc                                                             <  1:3640802/93‑1 (MQ=255)

                            gcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTc                                                             <  2:3769767/93‑1 (MQ=255)

                            gcagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTc                                                             <  1:195512/93‑1 (MQ=255)

                             cagcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTcc                                                            <  2:2935788/93‑1 (MQ=255)

                              agcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCg                                                           <  1:3195891/93‑1 (MQ=255)

                               gcGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCgg                                                          <  1:3878509/93‑1 (MQ=255)

                                cGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGa                                                         >  2:1470642/1‑93 (MQ=255)

                                cGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGa                                                         <  2:3473506/93‑1 (MQ=255)

                                 gACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaa                                                        <  2:3272967/93‑1 (MQ=255)

                                 gACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaa                                                        >  1:1711628/1‑93 (MQ=255)

                                 gACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaa                                                        >  2:1119933/1‑93 (MQ=255)

                                  aCCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaaa                                                       >  2:914301/1‑93 (MQ=255)

                                   ccATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaaaa                                                      >  2:2577496/1‑93 (MQ=255)

                                   ccATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaaaa                                                      <  2:129720/93‑1 (MQ=255)

                                    cATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGaaaaa                                                     >  1:2503136/1‑93 (MQ=255)

                                     aTCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAAc                                                    <  1:2289398/93‑1 (MQ=255)

                                     aTCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAAc                                                    >  2:1075775/1‑93 (MQ=255)

                                      tCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACt                                                   >  2:4326842/1‑93 (MQ=255)

                                      tCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACt                                                   >  1:1199717/1‑93 (MQ=255)

                                       cTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTg                                                  <  1:4362268/93‑1 (MQ=255)

                                        tGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGa                                                 <  1:3671963/93‑1 (MQ=255)

                                        tGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGa                                                 <  1:3820693/93‑1 (MQ=255)

                                          gTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATc                                               <  2:1632692/93‑1 (MQ=255)

                                            gcgcgcCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATccc                                             <  1:958533/93‑1 (MQ=255)

                                                  cTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCcgat                                       >  2:1204036/1‑93 (MQ=255)

                                                   tGGCTGCGCACTTATGCTCTGCGGACGTTTATCACCAACCTCTCGAATAACTCCGGCCTTTCCCACTTCCCGGAAAAACTGATCCCGCcgatg                                      <  2:1817830/93‑1 (MQ=255)

                                                    ggCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCcgatga                                     <  1:271018/93‑1 (MQ=255)

                                                     gCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCgatgat                                    >  1:739075/1‑93 (MQ=255)

                                                      cTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATc                                   >  2:2139651/1‑93 (MQ=255)

                                                       tGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATcc                                  >  1:3769272/1‑93 (MQ=255)

                                                        gCGGACCTATGGTCTGCCGACGCTTATCACCACCCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCt                                 <  1:359423/93‑1 (MQ=255)

                                                         cGGCCCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTCCCGAAAAAACTGATCCCGCCGATGATCCTc                                <  2:3678423/93‑1 (MQ=255)

                                                           gACCTATGGTCTGCCGACGTTTATCACCACCCGCTCAAATAACTACGGCCCTTCCCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCaa                              <  2:2828241/93‑1 (MQ=255)

                                                           gACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCaa                              >  1:2286651/1‑93 (MQ=255)

                                                           gACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCaa                              >  2:3356984/1‑93 (MQ=255)

                                                           gACCTATGGTCGGCCGACGCTTATCCCCACCCGCTCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCaa                              <  1:143879/93‑1 (MQ=255)

                                                            aCCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAc                             >  2:1556904/1‑93 (MQ=255)

                                                            aCCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAc                             >  1:2192986/1‑93 (MQ=255)

                                                             ccTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAcg                            >  1:1894550/1‑93 (MQ=255)

                                                             ccTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAcg                            >  2:829830/1‑93 (MQ=255)

                                                             ccTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAcg                            >  2:1866930/1‑93 (MQ=255)

                                                             ccTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCACAAcg                            >  1:2440098/1‑93 (MQ=255)

                                                             ccTAGGGTCTGCCGACGTTTATCCCCACCCGCTCGAATAACTACGGCCCTTCCCCCTTCCGGAAAAAACTGATCCCGCCGATGATCCTCAAcg                            <  2:3378667/93‑1 (MQ=255)

                                                              cTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAcgc                           >  1:3791781/1‑93 (MQ=255)

                                                                aTGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACgcgc                         <  1:3908398/93‑1 (MQ=255)

                                                                  ggTCTGCCGACTCTTATCCCAAACCGCCCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTg                       <  1:537774/93‑1 (MQ=255)

                                                                      tGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCgg                   >  1:1341721/1‑93 (MQ=255)

                                                                         cGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCgggca                >  1:3088979/1‑91 (MQ=255)

                                                                         cGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTa                >  1:4666345/1‑93 (MQ=255)

                                                                          gACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGGaa               >  2:3612823/1‑93 (MQ=255)

                                                                            cctttATCACCACCCGCTCAAATACCTACGGCCCTTCCCTCTTTCGGGAAAAACTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAAt             <  2:4431929/90‑1 (MQ=255)

                                                                            cGCTTATCACCACCCCCCGAAATAACTGCGCCCTTTCCCTCTTACCGAAAAACCTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAAt             <  2:2700577/93‑1 (MQ=255)

                                                                            cGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAAt             >  2:1509626/1‑93 (MQ=255)

                                                                             gCTTATCACCAACCGCTCGAATAACTACGTCCCTTACCACTTTCCGGAAAAACTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATc            <  1:1286381/93‑1 (MQ=255)

                                                                             gCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATc            >  1:1048514/1‑93 (MQ=255)

                                                                              cTTATCCCCAACCGCTCAAATAACTCGGCCCCTTCCCACTTCCCGAAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCg           <  2:4490024/93‑1 (MQ=255)

                                                                               ttATCCCCAACCGCTCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGc          <  2:2032235/93‑1 (MQ=255)

                                                                               taaccACCAACCGCTCGAATAACAACGCCCCTTCCCTCTTTCGGAAAAACCGGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGc          <  2:4139289/89‑1 (MQ=255)

                                                                                tATCACCAACCGCTCTAATAACTACGGCCCTTACCACTTTCCGAAAAAACGGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCt         <  1:1407164/93‑1 (MQ=255)

                                                                                  tCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGc       >  1:3960390/1‑93 (MQ=255)

                                                                                   cACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGGAAATCGCTGcc      >  1:2601994/1‑93 (MQ=255)

                                                                                    aCCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCg     >  2:2214871/1‑93 (MQ=255)

                                                                                    aCCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGGAAATCGCTGCCg     >  1:4004236/1‑93 (MQ=255)

                                                                                       aaCTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGAAAATCGCTGCCGGta  >  2:3934954/1‑93 (MQ=255)

                                                                                       aaCCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGta  >  2:4061820/1‑93 (MQ=255)

                                                                                       aaCCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGAGCTGGCGGGGAAATAGTTGCCGGtt  >  2:4214393/1‑92 (MQ=255)

AGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCTGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTA  >  NC_000913/3970602‑3970781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCTGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTATAT  >  NC_000913/3970599‑3970784

CCGAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACT                                                                                              <  SRR4302535.4404116/94‑1 (MQ=60)

 CGAGCAGCCCTTATTCCGCGTCAAAAGGCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCG                                                                                             >  SRR4302535.3126305/1‑94 (MQ=60)

 CGAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCG                                                                                             <  SRR4302535.924485/94‑1 (MQ=60)

 CGAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCG                                                                                             >  SRR4302535.769285/1‑94 (MQ=60)

  GAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGC                                                                                            <  SRR4302535.4644790/94‑1 (MQ=60)

     CAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCG                                                                                         <  SRR4302535.3426691/94‑1 (MQ=60)

     CAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCG                                                                                         <  SRR4302535.4430322/94‑1 (MQ=60)

     CAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCG                                                                                         >  SRR4302535.247893/1‑94 (MQ=60)

      AGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCAGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGA                                                                                        >  SRR4302535.2797224/1‑94 (MQ=60)

       GCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAA                                                                                       <  SRR4302535.700780/94‑1 (MQ=60)

       GCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAA                                                                                       >  SRR4302535.3846496/1‑94 (MQ=60)

        CCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAAT                                                                                      >  SRR4302535.465908/1‑94 (MQ=60)

         CCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATA                                                                                     >  SRR4302535.18503/1‑94 (MQ=60)

          CTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAA                                                                                    >  SRR4302535.3944268/1‑94 (MQ=60)

          CTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAA                                                                                    >  SRR4302535.1131698/1‑94 (MQ=60)

           TTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAC                                                                                   <  SRR4302535.524731/94‑1 (MQ=60)

           TTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAC                                                                                   >  SRR4302535.3475447/1‑94 (MQ=60)

           TTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAC                                                                                   >  SRR4302535.1948793/1‑94 (MQ=60)

           TTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAAC                                                                                   <  SRR4302535.3773418/94‑1 (MQ=60)

            TATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACT                                                                                  <  SRR4302535.4014729/94‑1 (MQ=60)

            TATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACT                                                                                  >  SRR4302535.2359348/1‑94 (MQ=60)

            TATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACT                                                                                  >  SRR4302535.3196343/1‑94 (MQ=60)

             ATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGGGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTA                                                                                 >  SRR4302535.3753869/1‑94 (MQ=60)

             ATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTA                                                                                 <  SRR4302535.143149/94‑1 (MQ=60)

              TTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCATCAACCGCTCGAATAACTAC                                                                                <  SRR4302535.2632778/94‑1 (MQ=60)

              TTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTAC                                                                                <  SRR4302535.4449130/94‑1 (MQ=60)

              TTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTAC                                                                                <  SRR4302535.1025521/94‑1 (MQ=60)

               TCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACG                                                                               <  SRR4302535.2628068/94‑1 (MQ=60)

               TCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACG                                                                               <  SRR4302535.1536494/94‑1 (MQ=60)

                CCGCGACAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGG                                                                              <  SRR4302535.1119735/94‑1 (MQ=60)

                 CGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGC                                                                             >  SRR4302535.4102354/1‑94 (MQ=60)

                  GCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCC                                                                            <  SRR4302535.400352/94‑1 (MQ=60)

                  GCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCC                                                                            >  SRR4302535.332535/1‑94 (MQ=60)

                    GTCAAAAGCCAGCAGCGACCATCTGGTGTGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCT                                                                          <  SRR4302535.663191/94‑1 (MQ=60)

                    GTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCT                                                                          <  SRR4302535.2048848/94‑1 (MQ=60)

                    GTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCT                                                                          >  SRR4302535.698627/1‑94 (MQ=60)

                     TCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTT                                                                         <  SRR4302535.2926317/94‑1 (MQ=60)

                     TCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTT                                                                         <  SRR4302535.3220233/94‑1 (MQ=60)

                     GCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTT                                                                         <  SRR4302535.1168282/94‑1 (MQ=60)

                      CAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTGTCACCAACCGCTCGAATAACTACGGCCCTTA                                                                        <  SRR4302535.2437457/94‑1 (MQ=60)

                      CAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTA                                                                        <  SRR4302535.221912/94‑1 (MQ=60)

                      CAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTA                                                                        >  SRR4302535.3276302/1‑94 (MQ=60)

                       AAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTAC                                                                       <  SRR4302535.578057/94‑1 (MQ=60)

                        AAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACC                                                                      >  SRR4302535.244052/1‑94 (MQ=60)

                        AAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACC                                                                      <  SRR4302535.3798839/94‑1 (MQ=60)

                        AAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACC                                                                      >  SRR4302535.4593805/1‑94 (MQ=60)

                        AAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACC                                                                      <  SRR4302535.879501/94‑1 (MQ=60)

                         AAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCA                                                                     >  SRR4302535.1306549/1‑94 (MQ=60)

                          AGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCAC                                                                    <  SRR4302535.1055575/94‑1 (MQ=60)

                           GCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACT                                                                   >  SRR4302535.1780058/1‑94 (MQ=60)

                            CCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTT                                                                  <  SRR4302535.2196413/94‑1 (MQ=60)

                             CAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTT                                                                 >  SRR4302535.3671720/1‑94 (MQ=60)

                             CAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTT                                                                 >  SRR4302535.436604/1‑94 (MQ=60)

                              AGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTC                                                                <  SRR4302535.1684844/94‑1 (MQ=60)

                              AGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTC                                                                >  SRR4302535.1152104/1‑94 (MQ=60)

                               GCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCC                                                               <  SRR4302535.3666022/94‑1 (MQ=60)

                               GCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCC                                                               <  SRR4302535.196855/94‑1 (MQ=60)

                                 AGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGG                                                             <  SRR4302535.3218006/94‑1 (MQ=60)

                                  GCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGA                                                            <  SRR4302535.3905139/94‑1 (MQ=60)

                                   CGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAA                                                           >  SRR4302535.1724006/1‑94 (MQ=60)

                                    GACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAA                                                          <  SRR4302535.269328/94‑1 (MQ=60)

                                     ACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAA                                                         <  SRR4302535.138739/94‑1 (MQ=60)

                                      CCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAA                                                        >  SRR4302535.2521186/1‑94 (MQ=60)

                                       CATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAAC                                                       <  SRR4302535.2473041/94‑1 (MQ=60)

                                        ATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACT                                                      >  SRR4302535.2576690/1‑94 (MQ=60)

                                        ATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACT                                                      <  SRR4302535.2305751/94‑1 (MQ=60)

                                        ATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACT                                                      >  SRR4302535.1208315/1‑94 (MQ=60)

                                         TCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTG                                                     <  SRR4302535.1388322/94‑1 (MQ=60)

                                          CTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGA                                                    <  SRR4302535.4387975/94‑1 (MQ=60)

                                          CTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGA                                                    <  SRR4302535.4392120/94‑1 (MQ=60)

                                           TGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGAT                                                   <  SRR4302535.3847053/94‑1 (MQ=60)

                                           TGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGAT                                                   <  SRR4302535.3697391/94‑1 (MQ=60)

                                             GTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCC                                                 <  SRR4302535.3847703/94‑1 (MQ=60)

                                               GCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCG                                               <  SRR4302535.965340/94‑1 (MQ=60)

                                                CGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGC                                              <  SRR4302535.3020893/94‑1 (MQ=60)

                                                CGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGC                                              >  SRR4302535.4003212/1‑94 (MQ=60)

                                                  CGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCG                                            >  SRR4302535.3256553/1‑94 (MQ=60)

                                                   GCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGA                                           >  SRR4302535.2305993/1‑94 (MQ=60)

                                                    CCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGAT                                          >  SRR4302535.3582873/1‑94 (MQ=60)

                                                    CCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGAT                                          >  SRR4302535.75237/1‑94 (MQ=60)

                                                     CTGGCTGCGGACCTATGGTCTGCCGACGCTTATCCCCACCCGCTCGAATAACTACGGCCCTTACCCCTTTCCGGAAAAACTGATCCCGCCGATG                                         <  SRR4302535.2658715/94‑1 (MQ=60)

                                                     CTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATG                                         >  SRR4302535.2369171/1‑94 (MQ=60)

                                                     CTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATG                                         <  SRR4302535.3555237/94‑1 (MQ=60)

                                                      TGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGA                                        >  SRR4302535.1136541/1‑94 (MQ=60)

                                                       GGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGAT                                       <  SRR4302535.272905/94‑1 (MQ=60)

                                                       GGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGAT                                       >  SRR4302535.744272/1‑94 (MQ=60)

                                                         CTGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCC                                     >  SRR4302535.3795288/1‑94 (MQ=60)

                                                          TGCGGACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCT                                    >  SRR4302535.3300102/1‑94 (MQ=60)

                                                           GCGGACCTATGGTCTGCCGACGCTTATCACCACCCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTC                                   <  SRR4302535.361912/94‑1 (MQ=60)

                                                             GGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAA                                 >  SRR4302535.2302987/1‑94 (MQ=60)

                                                             GGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAA                                 >  SRR4302535.2302988/1‑94 (MQ=60)

                                                              GACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAC                                >  SRR4302535.2208615/1‑94 (MQ=60)

                                                              GACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAAC                                <  SRR4302535.230998/94‑1 (MQ=60)

                                                              GACCTATGGTCGGCCGACGCTTATCCCCACCCGCTCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCAAC                                <  SRR4302535.144881/94‑1 (MQ=60)

                                                               ACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACG                               >  SRR4302535.1908187/1‑94 (MQ=60)

                                                               ACCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCACAACG                               >  SRR4302535.2457675/1‑94 (MQ=60)

                                                                CCTATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGC                              >  SRR4302535.3817923/1‑94 (MQ=60)

                                                                  TATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGC                            >  SRR4302535.3917741/1‑94 (MQ=60)

                                                                   ATGTTCCGCCGCCGCTTATCACCACCCGCGCGAATATCTCCGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCT                           <  SRR4302535.1028299/94‑1 (MQ=60)

                                                                   ATGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCT                           <  SRR4302535.3935169/94‑1 (MQ=60)

                                                                    TGGTCTGCCGACGCTTATCACCACCCGCCGGAATACCTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTG                          <  SRR4302535.2664107/94‑1 (MQ=60)

                                                                    TGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTG                          >  SRR4302535.1161021/1‑94 (MQ=60)

                                                                    TGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTG                          >  SRR4302535.3431538/1‑94 (MQ=60)

                                                                    TGGTCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTG                          <  SRR4302535.4676658/94‑1 (MQ=60)

                                                                     GGTCTGCCGACTCTTATCCCAAACCGCCCGAATAACTACGGCCCTTACCACTTCCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGG                         <  SRR4302535.541530/94‑1 (MQ=60)

                                                                       TCTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCG                       >  SRR4302535.1245676/1‑94 (MQ=60)

                                                                        CTGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGG                      >  SRR4302535.1351294/1‑94 (MQ=60)

                                                                         TGCCTACGCTTATCACCAGCCGCTCAAATAACTACGCCCCTACCCACTTCCCGGAAAAACATATCCCGCCGATGATCCTCAACGCGCTGGCGGG                     <  SRR4302535.2768122/94‑1 (MQ=32)

                                                                         TGCCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGG                     >  SRR4302535.1458491/1‑94 (MQ=60)

                                                                          GCCGACGCTTATCCCCAACCGCTCGAAAAACTACGGCCCTTACCACTTTCCGGAAAAACTAATCCCGCCGATGATCCTCAACGCGCTGGCGGGT                    <  SRR4302535.1895585/94‑1 (MQ=60)

                                                                           CCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTA                   >  SRR4302535.4697887/1‑94 (MQ=60)

                                                                           CCGACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGCA                   >  SRR4302535.3110493/1‑94 (MQ=60)

                                                                              ACGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAAT                >  SRR4302535.3862695/1‑94 (MQ=60)

                                                                               CGCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATC               >  SRR4302535.1055951/1‑94 (MQ=60)

                                                                                GCTTATCACCAACCGCTCGAATAACTACGTCCCTTACCACTTTCCGGAAAAACTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCG              <  SRR4302535.1295548/94‑1 (MQ=60)

                                                                                GCTTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCG              >  SRR4302535.670466/1‑94 (MQ=60)

                                                                                CCTTATCACCACCCGCTTGAATAACTCCGGCCCTTGCCACTTTCCGGAAAACCTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCG              <  SRR4302535.1692805/94‑1 (MQ=60)

                                                                                  TTATCACCACCCGCTCAAATACCTACGGCCCTTACCACTTCCCGAAAAAACTGACCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCT            <  SRR4302535.98747/94‑1 (MQ=60)

                                                                                  TTATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCT            <  SRR4302535.3624827/94‑1 (MQ=60)

                                                                                   TATCACCAACCGCTCTAATAACTACGGCCCTTACCACTTTCCGAAAAAACGGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTG           <  SRR4302535.1417217/94‑1 (MQ=60)

                                                                                    ATCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGC          >  SRR4302535.3987437/1‑94 (MQ=60)

                                                                                     TCACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGGAAATCGCTGCC         >  SRR4302535.2620674/1‑94 (MQ=60)

                                                                                      CACCACCCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCG        <  SRR4302535.621497/94‑1 (MQ=60)

                                                                                      CACCAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGGAAATCGCTGCCG        >  SRR4302535.4031629/1‑94 (MQ=60)

                                                                                         CAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTA     >  SRR4302535.4546305/1‑94 (MQ=60)

                                                                                         CAACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGGA     >  SRR4302535.3383408/1‑94 (MQ=60)

                                                                                          AACCGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATAGCTGCCGGTAT    >  SRR4302535.3265026/1‑94 (MQ=60)

                                                                                          AACCGCTCGAATAACTACGCCCCTTACCACTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTAT    <  SRR4302535.2760521/94‑1 (MQ=60)

                                                                                            CCGCTCGAATAACTACGGCCCTTACCCCTTTCCGGAAAAACTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTATAT  <  SRR4302535.1546462/94‑1 (MQ=60)

                                                                                            CCGCTCAAATAACTACGCCCCTTCCCACTTTCCGAAAAACCTGATCCCGCCGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTATAT  <  SRR4302535.3063079/94‑1 (MQ=60)

CCGAGCAGCCCTTATTCCGCGTCAAAAGCCAGCAGCGACCATCTGGTGCGCGCCTGGCTGCGGACCTATGGTCTGCCGACGCTTATCACCAACTGCTCGAATAACTACGGCCCTTACCACTTTCCGGAAAAACTGATCCCGCTGATGATCCTCAACGCGCTGGCGGGTAAATCGCTGCCGGTATAT  >  NC_000913/3970599‑3970784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`