Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,535	+G	coding (1036/1386 nt)	yifK →	putative amino acid/amine transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,534	1	.	G	99.6%	790.9 / ‑4.5	245	coding (1035/1386 nt)	yifK	putative amino acid/amine transport protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (130/114); total (131/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 265 total aligned reads displayed.

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT  >  NC_000913/3979442‑3979622

aaaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAtt                                                                                           <  1:3846826/93‑1 (MQ=255)

aaaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAtt                                                                                           <  1:4606064/93‑1 (MQ=255)

aaaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAtt                                                                                           <  2:2871286/93‑1 (MQ=255)

  aaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑gg                                                                                        <  2:59154/93‑2 (MQ=255)

    aCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑ggct                                                                                      <  1:690426/93‑4 (MQ=255)

       gTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAt                                                                                    >  1:3076781/1‑93 (MQ=255)

       gTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAt                                                                                    <  1:1511236/93‑1 (MQ=255)

        tCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTTCTAATTGGCTCATg                                                                                   >  2:3285384/1‑93 (MQ=255)

        tCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATg                                                                                   >  2:212687/1‑93 (MQ=255)

        tCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATg                                                                                   <  1:645749/93‑1 (MQ=255)

        tCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATg                                                                                   >  1:2461991/1‑93 (MQ=255)

        tCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATg                                                                                   >  2:1256810/1‑93 (MQ=255)

         cAGTTACCGGCGGCAATGGCGAAAGTTGCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGc                                                                                  <  2:294322/93‑1 (MQ=255)

           gTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCt                                                                                <  1:2027207/93‑1 (MQ=255)

           gTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCt                                                                                >  2:2710045/1‑93 (MQ=255)

             tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTga                                                                              <  2:44080/93‑2 (MQ=255)

               ccGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaac                                                                            <  1:1186033/93‑4 (MQ=255)

                cggcggCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaact                                                                           >  2:4058807/1‑89 (MQ=255)

                  gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                        >  2:1939110/1‑93 (MQ=255)

                   cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAca                                                                       <  1:4353851/93‑1 (MQ=255)

                    ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                      <  2:1258605/93‑1 (MQ=255)

                     gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                     >  1:2807539/1‑93 (MQ=255)

                      cAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatca                                                                    <  2:1480817/93‑1 (MQ=255)

                       aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                   >  2:4335305/1‑93 (MQ=255)

                        aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                  >  1:4450042/1‑93 (MQ=255)

                          ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                <  2:2920826/93‑1 (MQ=255)

                           gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTccc                                                               >  1:3134935/1‑93 (MQ=255)

                           gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTccc                                                               >  2:3668336/1‑93 (MQ=255)

                             gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                             <  1:2480016/93‑1 (MQ=255)

                             gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                             >  1:3471038/1‑93 (MQ=255)

                             gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAATACATCATTCCCaa                                                             >  2:644063/1‑93 (MQ=255)

                              aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCTTTCCCaac                                                            >  2:3109439/1‑92 (MQ=255)

                              aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                            >  1:2257181/1‑93 (MQ=255)

                               aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                           <  2:1770218/93‑1 (MQ=255)

                               aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                           >  1:4417671/1‑93 (MQ=255)

                                aGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATcc                                                          <  2:2336932/93‑1 (MQ=255)

                                aGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATcc                                                          <  1:1935004/93‑1 (MQ=255)

                                    tCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAg                                                      <  1:4511646/93‑1 (MQ=255)

                                          cACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtg                                                <  2:840961/93‑1 (MQ=255)

                                           aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                               >  2:3957669/1‑93 (MQ=255)

                                           aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                               >  1:1704493/1‑93 (MQ=255)

                                           aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                               <  2:4533711/93‑1 (MQ=255)

                                           aCGGCGTACCGGTTGCGGGGGTGGCACGGTCTATTGCTTTTCTGCTAATTGGCTCATGGCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                               >  2:2768595/1‑93 (MQ=255)

                                            cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                              <  1:1404156/93‑1 (MQ=255)

                                                gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                          >  1:3566090/1‑93 (MQ=255)

                                                gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                          <  1:2586788/93‑1 (MQ=255)

                                                gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                          <  1:2319514/93‑1 (MQ=255)

                                                 tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                         >  2:3449914/1‑93 (MQ=255)

                                                 tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                         >  2:138032/1‑93 (MQ=255)

                                                 tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                         >  2:4121137/1‑93 (MQ=255)

                                                  cccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtcta                                        <  2:1685904/92‑1 (MQ=255)

                                                  aCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtcta                                        >  1:1274484/1‑93 (MQ=255)

                                                   ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                       >  2:52369/1‑93 (MQ=255)

                                                   ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                       <  2:1652225/93‑1 (MQ=255)

                                                    cGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                      >  1:3349756/1‑93 (MQ=255)

                                                     ggTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgt                                     <  1:242424/93‑1 (MQ=255)

                                                     ggTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgt                                     >  1:2907026/1‑93 (MQ=255)

                                                          cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                <  1:1824715/93‑1 (MQ=255)

                                                           gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                               <  2:551185/93‑1 (MQ=255)

                                                           gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                               >  2:3720895/1‑93 (MQ=255)

                                                             gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTg                             >  1:1071619/1‑93 (MQ=255)

                                                               gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                           <  2:2566468/93‑1 (MQ=255)

                                                               gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                           >  2:884478/1‑93 (MQ=255)

                                                                 ggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                         >  2:2620820/1‑93 (MQ=255)

                                                                    aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                      >  1:776325/1‑93 (MQ=255)

                                                                    aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                      >  2:3355526/1‑93 (MQ=255)

                                                                     gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                     >  1:3916366/1‑93 (MQ=255)

                                                                     gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                     >  2:3416089/1‑93 (MQ=255)

                                                                     gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                     >  2:327303/1‑93 (MQ=255)

                                                                      tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                    <  2:2367204/93‑1 (MQ=255)

                                                                      tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                    >  1:2263109/1‑93 (MQ=255)

                                                                      tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                    >  1:3264662/1‑93 (MQ=255)

                                                                        tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                  <  2:4285544/93‑1 (MQ=255)

                                                                         cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                 <  1:1332106/93‑1 (MQ=255)

                                                                          tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                <  2:2250951/93‑1 (MQ=255)

                                                                           aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg               <  1:1004082/93‑1 (MQ=255)

                                                                           aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg               <  1:869690/93‑1 (MQ=255)

                                                                           aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg               >  1:910918/1‑93 (MQ=255)

                                                                           aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg               >  2:2849899/1‑93 (MQ=255)

                                                                            ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg              <  2:3439930/93‑1 (MQ=255)

                                                                            ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg              >  1:1800122/1‑93 (MQ=255)

                                                                               cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa           <  2:3812563/93‑1 (MQ=255)

                                                                                tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt          >  2:3018596/1‑93 (MQ=255)

                                                                                 aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg         >  1:1168100/1‑93 (MQ=255)

                                                                                 aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg         <  1:354639/93‑1 (MQ=255)

                                                                                 aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg         >  2:1081033/1‑93 (MQ=255)

                                                                                 aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCACAATCCGCAGCGCGTGTTTGTCTACGTCTACAGTGCCAGCGTGCCTCCGGGGATg         >  2:2073016/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  2:2555879/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  1:606022/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  1:162908/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  1:2658508/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        <  1:3020419/93‑1 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  1:3610463/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        >  1:3951656/1‑93 (MQ=255)

                                                                                  ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg        <  1:4143734/93‑1 (MQ=255)

                                                                                    cTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTg      >  2:772070/1‑93 (MQ=255)

                                                                                        tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt  <  1:2551564/93‑1 (MQ=255)

                                                                                        tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt  >  2:4635811/1‑93 (MQ=255)

                                                                                        tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt  <  2:1580219/93‑1 (MQ=255)

                                                                                        tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt  <  1:4302881/93‑1 (MQ=255)

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT  >  NC_000913/3979442‑3979622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG  >  NC_000913/3979442‑3979628

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G                                                                                               <  SRR4302535.3873309/94‑1 (MQ=60)

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G                                                                                               <  SRR4302535.4637370/94‑1 (MQ=60)

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G                                                                                               >  SRR4302535.1520152/1‑94 (MQ=60)

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G                                                                                               >  SRR4302535.3136493/1‑94 (MQ=60)

 AAAGCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GG                                                                                              <  SRR4302535.1800997/94‑1 (MQ=60)

 AAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GG                                                                                              >  SRR4302535.2654425/1‑94 (MQ=60)

 AAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GG                                                                                              <  SRR4302535.1767391/94‑1 (MQ=60)

    ACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC                                                                                            >  SRR4302535.736141/1‑94 (MQ=60)

    ACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC                                                                                            <  SRR4302535.695266/94‑1 (MQ=60)

      CGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACTGCGTACCGGTTGCGGGTTTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAT                                                                                          >  SRR4302535.384825/1‑94 (MQ=60)

      CGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAT                                                                                          >  SRR4302535.3098244/1‑94 (MQ=60)

      CGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAT                                                                                          <  SRR4302535.4511775/94‑1 (MQ=60)

       GTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATG                                                                                         <  SRR4302535.1522123/94‑1 (MQ=60)

       GTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATG                                                                                         >  SRR4302535.2479740/1‑94 (MQ=60)

        TCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGC                                                                                        <  SRR4302535.650255/94‑1 (MQ=60)

         CAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTTCTAATTGGCTCATGCT                                                                                       >  SRR4302535.2878201/1‑94 (MQ=60)

         CAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCC                                                                                       <  SRR4302535.1297177/94‑1 (MQ=60)

          AGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT                                                                                      <  SRR4302535.1234931/94‑1 (MQ=60)

          AGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTACTGCTATTCTGCTAATTGGCTCATGCCT                                                                                      >  SRR4302535.3277640/1‑94 (MQ=60)

           GTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTG                                                                                     <  SRR4302535.4429315/94‑1 (MQ=60)

           GTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTG                                                                                     <  SRR4302535.2041815/94‑1 (MQ=60)

              ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                 <  SRR4302535.4342005/94‑1 (MQ=60)

              ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                 >  SRR4302535.2735284/1‑94 (MQ=60)

               CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                <  SRR4302535.1194504/94‑1 (MQ=60)

                  GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                             <  SRR4302535.3904288/94‑1 (MQ=60)

                   CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                            >  SRR4302535.1306799/1‑94 (MQ=60)

                   CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                            <  SRR4302535.4383649/94‑1 (MQ=60)

                    GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                           >  SRR4302535.2827496/1‑94 (MQ=60)

                    GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                           <  SRR4302535.1986445/94‑1 (MQ=60)

                     GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                          <  SRR4302535.922070/94‑1 (MQ=60)

                       AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATT                                                                        >  SRR4302535.4480564/1‑94 (MQ=60)

                        ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                       >  SRR4302535.3947870/1‑94 (MQ=60)

                        ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                       <  SRR4302535.3093411/94‑1 (MQ=60)

                        ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                       <  SRR4302535.3102577/94‑1 (MQ=60)

                         TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCC                                                                      <  SRR4302535.471048/94‑1 (MQ=60)

                          GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                     >  SRR4302535.3156757/1‑94 (MQ=60)

                           GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATCGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                    <  SRR4302535.3317979/94‑1 (MQ=60)

                            CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                   >  SRR4302535.3495158/1‑94 (MQ=60)

                            CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                   >  SRR4302535.2299135/1‑94 (MQ=60)

                             GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                  <  SRR4302535.2497900/94‑1 (MQ=60)

                             GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                  >  SRR4302535.2273247/1‑94 (MQ=60)

                             GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                  >  SRR4302535.2273420/1‑94 (MQ=60)

                             GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGCGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                  >  SRR4302535.2583789/1‑94 (MQ=60)

                              AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                 >  SRR4302535.4447915/1‑94 (MQ=60)

                               AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                <  SRR4302535.3988004/94‑1 (MQ=60)

                               AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                <  SRR4302535.2666464/94‑1 (MQ=60)

                                AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                               <  SRR4302535.1948941/94‑1 (MQ=60)

                                 agTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGC                                                              <  SRR4302535.2300430/92‑1 (MQ=60)

                                   TTCCCGTCACGGCGTACCGGTTGTGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                            <  SRR4302535.2486074/94‑1 (MQ=60)

                                   TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                            <  SRR4302535.4546489/94‑1 (MQ=60)

                                   TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                            <  SRR4302535.662977/94‑1 (MQ=60)

                                    TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                           <  SRR4302535.4542590/94‑1 (MQ=60)

                                    TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                           <  SRR4302535.3765982/94‑1 (MQ=60)

                                    TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                           >  SRR4302535.2493822/1‑94 (MQ=60)

                                       CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTTTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                        >  SRR4302535.3500345/1‑94 (MQ=60)

                                        GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                       <  SRR4302535.2953457/94‑1 (MQ=60)

                                        GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                       >  SRR4302535.1058896/1‑94 (MQ=60)

                                          CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                     >  SRR4302535.1716816/1‑94 (MQ=60)

                                          CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                     >  SRR4302535.863343/1‑94 (MQ=60)

                                            CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                   <  SRR4302535.3585611/94‑1 (MQ=60)

                                            CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                   <  SRR4302535.4193754/94‑1 (MQ=60)

                                            CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                   <  SRR4302535.1414173/94‑1 (MQ=60)

                                               CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                >  SRR4302535.2545194/1‑94 (MQ=60)

                                               CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                >  SRR4302535.3590815/1‑94 (MQ=60)

                                                GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                               <  SRR4302535.2605365/94‑1 (MQ=60)

                                                GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                               <  SRR4302535.2336118/94‑1 (MQ=60)

                                                GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                               >  SRR4302535.2375874/1‑94 (MQ=60)

                                                GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                               >  SRR4302535.4441883/1‑94 (MQ=60)

                                                GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                               <  SRR4302535.2466875/94‑1 (MQ=60)

                                                 TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                              >  SRR4302535.1283582/1‑94 (MQ=60)

                                                   CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                            >  SRR4302535.2865890/1‑94 (MQ=60)

                                                   CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                            >  SRR4302535.3373089/1‑94 (MQ=60)

                                                    CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                           >  SRR4302535.2927572/1‑94 (MQ=60)

                                                     GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                          <  SRR4302535.244086/94‑1 (MQ=60)

                                                      GTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCAGCAGAGTGTGTTTGTCTACGTCT                                         >  SRR4302535.612697/1‑94 (MQ=60)

                                                        TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                       <  SRR4302535.2183539/94‑1 (MQ=60)

                                                         GCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACA                                      <  SRR4302535.3714943/94‑1 (MQ=60)

                                                          CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                     >  SRR4302535.1188051/1‑94 (MQ=60)

                                                          CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                     <  SRR4302535.1837888/94‑1 (MQ=60)

                                                          CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAGGCAT‑GAACTACATCATTCCCAATACGCAGCGTGTGTTTGTCTACGTCTACAG                                     >  SRR4302535.895295/1‑94 (MQ=60)

                                                           GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                    <  SRR4302535.1935188/94‑1 (MQ=60)

                                                            GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                   >  SRR4302535.1079233/1‑94 (MQ=60)

                                                              TGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                 <  SRR4302535.2222184/94‑1 (MQ=60)

                                                                  GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                             <  SRR4302535.1965920/94‑1 (MQ=60)

                                                                   CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                            >  SRR4302535.781781/1‑94 (MQ=60)

                                                                    AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                           >  SRR4302535.87918/1‑94 (MQ=60)

                                                                    AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                           >  SRR4302535.3592338/1‑94 (MQ=60)

                                                                    AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                           >  SRR4302535.3943166/1‑94 (MQ=60)

                                                                    AATATCTATTTCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                           <  SRR4302535.1596074/94‑1 (MQ=60)

                                                                     GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                          >  SRR4302535.3287334/1‑94 (MQ=60)

                                                                     GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                          >  SRR4302535.2279232/1‑94 (MQ=60)

                                                                      TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                         >  SRR4302535.3443853/1‑94 (MQ=60)

                                                                      TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                         >  SRR4302535.2435410/1‑94 (MQ=60)

                                                                       ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                        <  SRR4302535.4146716/94‑1 (MQ=60)

                                                                       ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                        >  SRR4302535.2514809/1‑94 (MQ=60)

                                                                        TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                       <  SRR4302535.1833445/94‑1 (MQ=60)

                                                                        TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                       >  SRR4302535.4488696/1‑94 (MQ=60)

                                                                         CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                      <  SRR4302535.1341609/94‑1 (MQ=60)

                                                                          TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                     >  SRR4302535.917395/1‑94 (MQ=60)

                                                                          TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                     >  SRR4302535.1478649/1‑94 (MQ=60)

                                                                          TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                     <  SRR4302535.1142730/94‑1 (MQ=60)

                                                                          TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                     >  SRR4302535.4654075/1‑94 (MQ=60)

                                                                           ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                    <  SRR4302535.1011211/94‑1 (MQ=60)

                                                                           ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                    <  SRR4302535.875852/94‑1 (MQ=60)

                                                                           ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                    >  SRR4302535.1813135/1‑94 (MQ=60)

                                                                            TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                   <  SRR4302535.841441/94‑1 (MQ=60)

                                                                              GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                 >  SRR4302535.3542820/1‑94 (MQ=60)

                                                                               CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGTT                <  SRR4302535.4455117/94‑1 (MQ=60)

                                                                               CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                >  SRR4302535.1631442/1‑94 (MQ=60)

                                                                               CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                <  SRR4302535.4514442/94‑1 (MQ=60)

                                                                               CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                >  SRR4302535.1378373/1‑94 (MQ=60)

                                                                               CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                >  SRR4302535.3603428/1‑94 (MQ=60)

                                                                                TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG               >  SRR4302535.4365257/1‑94 (MQ=60)

                                                                                TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG               >  SRR4302535.1176439/1‑94 (MQ=60)

                                                                                TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTACGGGGATG               >  SRR4302535.394937/1‑94 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              <  SRR4302535.3381997/94‑1 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              <  SRR4302535.2939945/94‑1 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              <  SRR4302535.357086/94‑1 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              >  SRR4302535.3635500/1‑94 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              >  SRR4302535.2677557/1‑94 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              >  SRR4302535.3978636/1‑94 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              >  SRR4302535.164046/1‑94 (MQ=60)

                                                                                 ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG              >  SRR4302535.610257/1‑94 (MQ=60)

                                                                                  TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT             <  SRR4302535.3041571/94‑1 (MQ=60)

                                                                                  TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT             <  SRR4302535.4172107/94‑1 (MQ=60)

                                                                                   TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG            <  SRR4302535.1681103/94‑1 (MQ=60)

                                                                                   TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG            <  SRR4302535.649888/94‑1 (MQ=60)

                                                                                   TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG            <  SRR4302535.1124833/94‑1 (MQ=60)

                                                                                    CTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC           <  SRR4302535.3221603/94‑1 (MQ=60)

                                                                                     TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC          <  SRR4302535.1833374/94‑1 (MQ=60)

                                                                                      GCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA         >  SRR4302535.701130/1‑94 (MQ=60)

                                                                                       CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT        <  SRR4302535.1225674/94‑1 (MQ=60)

                                                                                        TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG       <  SRR4302535.2569937/94‑1 (MQ=60)

                                                                                        TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG       <  SRR4302535.4332318/94‑1 (MQ=60)

                                                                                         AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG      >  SRR4302535.447453/1‑94 (MQ=60)

                                                                                         AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG      >  SRR4302535.3001306/1‑94 (MQ=60)

                                                                                          ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT     >  SRR4302535.1576724/1‑94 (MQ=60)

                                                                                          ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT     >  SRR4302535.401801/1‑94 (MQ=60)

                                                                                          ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT     >  SRR4302535.2157425/1‑94 (MQ=60)

                                                                                          ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT     <  SRR4302535.3131711/94‑1 (MQ=60)

                                                                                          ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT     >  SRR4302535.978096/1‑94 (MQ=60)

                                                                                            G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG  >  SRR4302535.4012511/1‑94 (MQ=60)

AAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG  >  NC_000913/3979442‑3979628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`