Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,546 Δ1 bp coding (1047/1386 nt) yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,5450G.100.0% 1134.8 / NA 247coding (1046/1386 nt)yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (137/110);  total (137/110)

Only 100 of 257 total aligned reads displayed.

TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979455‑3979633
                                                                                           |                                                                                        
tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTga                                                                                         <  2:44080/93‑2 (MQ=255)
 aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaa                                                                                        >  2:4362925/1‑91 (MQ=255)
 aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaa                                                                                        <  1:4312505/93‑3 (MQ=255)
     gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                                   <  1:3877659/93‑1 (MQ=255)
       ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 >  2:2347665/1‑93 (MQ=255)
        gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                                <  1:915569/93‑1 (MQ=255)
        gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                                <  2:1047001/93‑1 (MQ=255)
          aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              >  2:1577531/1‑93 (MQ=255)
           aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                             <  1:3081099/93‑1 (MQ=255)
            tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                            >  1:3921061/1‑93 (MQ=255)
            tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                            <  1:467776/93‑1 (MQ=255)
             ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           <  2:2920826/93‑1 (MQ=255)
             ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           <  2:2822869/93‑1 (MQ=255)
              gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTccc                                                                          >  1:3134935/1‑93 (MQ=255)
              gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATCGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTccc                                                                          <  1:3295073/93‑1 (MQ=255)
                gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                                        <  1:2480016/93‑1 (MQ=255)
                gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                                        <  2:1969571/93‑1 (MQ=255)
                 aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                                       >  1:2257351/1‑93 (MQ=255)
                 aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                                       >  2:4117519/1‑93 (MQ=255)
                  aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:1770218/93‑1 (MQ=255)
                    agttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCg                                                                    <  1:2284111/91‑1 (MQ=255)
                     tttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                                   >  2:1238807/1‑93 (MQ=255)
                     tttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                                   <  2:1616479/93‑1 (MQ=255)
                      ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  2:2078107/93‑1 (MQ=255)
                      ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  >  2:2574185/1‑93 (MQ=255)
                       tCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAg                                                                 <  1:4511646/93‑1 (MQ=255)
                          cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              >  2:515477/1‑93 (MQ=255)
                           gTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTTTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtg                                                             >  1:3476186/1‑93 (MQ=255)
                           gTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtg                                                             <  1:2932811/93‑1 (MQ=255)
                            tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgt                                                            >  1:1051418/1‑93 (MQ=255)
                             cACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtg                                                           <  2:840961/93‑1 (MQ=255)
                              aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                                          <  2:4533711/93‑1 (MQ=255)
                               cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  1:3560920/93‑1 (MQ=255)
                               cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  1:4165233/93‑1 (MQ=255)
                               cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  2:3346495/93‑1 (MQ=255)
                                ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        <  2:3932945/93‑1 (MQ=255)
                                 gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                                       >  2:3369096/1‑93 (MQ=255)
                                   gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:2586788/93‑1 (MQ=255)
                                   gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     >  2:2563069/1‑93 (MQ=255)
                                   gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:2449225/93‑1 (MQ=255)
                                   gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     >  1:2526991/1‑93 (MQ=255)
                                    tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  2:3449914/1‑93 (MQ=255)
                                    tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  1:4411690/1‑93 (MQ=255)
                                    tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  2:138032/1‑93 (MQ=255)
                                     aCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtcta                                                   >  1:1274484/1‑93 (MQ=255)
                                      ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  >  2:3426089/1‑93 (MQ=255)
                                      ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  >  2:1677838/1‑93 (MQ=255)
                                       cGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                                 >  1:2845715/1‑93 (MQ=255)
                                         gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  2:4607690/1‑93 (MQ=255)
                                         gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  2:3273956/1‑93 (MQ=255)
                                           tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             <  2:3212944/93‑1 (MQ=255)
                                            gCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtctac                                            <  1:3689407/93‑1 (MQ=255)
                                             cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                           <  1:1824715/93‑1 (MQ=255)
                                              gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  2:3720895/1‑93 (MQ=255)
                                              gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  1:1179634/1‑93 (MQ=255)
                                                  gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:884478/1‑93 (MQ=255)
                                                  gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:2851721/1‑93 (MQ=255)
                                                    ggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                                    >  2:2620820/1‑93 (MQ=255)
                                                     gCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGc                                   <  1:1951873/93‑1 (MQ=255)
                                                       aatATCTATTTCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                                 <  1:1584642/91‑1 (MQ=255)
                                                        gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:3567601/1‑93 (MQ=255)
                                                          aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              >  1:2418004/1‑93 (MQ=255)
                                                           tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                             <  2:4285544/93‑1 (MQ=255)
                                                           tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                             >  2:692896/1‑93 (MQ=255)
                                                            cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            >  1:4458121/1‑93 (MQ=255)
                                                            cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  1:1332106/93‑1 (MQ=255)
                                                             tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           <  1:1134634/93‑1 (MQ=255)
                                                             tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCGTcc                           >  2:2162996/1‑93 (MQ=255)
                                                              aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          >  1:1468132/1‑93 (MQ=255)
                                                              aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          <  1:1004082/93‑1 (MQ=255)
                                                              aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          <  1:869690/93‑1 (MQ=255)
                                                               ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         >  2:3998316/1‑93 (MQ=255)
                                                               ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         >  2:2261760/1‑93 (MQ=255)
                                                               ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         <  1:835582/93‑1 (MQ=255)
                                                                tGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        <  2:2388549/93‑1 (MQ=255)
                                                                tGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        <  2:3609708/93‑1 (MQ=255)
                                                                  cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggggt                      <  1:4424810/93‑2 (MQ=255)
                                                                   tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     >  2:3018596/1‑93 (MQ=255)
                                                                    aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg                    <  1:3358616/93‑1 (MQ=255)
                                                                     ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  1:3951656/1‑93 (MQ=255)
                                                                     ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  1:2658508/1‑93 (MQ=255)
                                                                     ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   <  1:3020419/93‑1 (MQ=255)
                                                                      tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  <  2:4139741/93‑1 (MQ=255)
                                                                      tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  <  2:1863133/93‑1 (MQ=255)
                                                                      tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  <  1:645385/93‑1 (MQ=255)
                                                                      tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  <  1:1669027/93‑1 (MQ=255)
                                                                       cTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTg                 >  2:772070/1‑93 (MQ=255)
                                                                        tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCCGCGTGCTTCCGGGGACGGTGc                >  2:548945/1‑93 (MQ=255)
                                                                        tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                <  1:1820224/93‑1 (MQ=255)
                                                                          cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              >  1:696248/1‑93 (MQ=255)
                                                                          cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              >  2:1448221/1‑93 (MQ=255)
                                                                           tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             >  2:271761/1‑93 (MQ=255)
                                                                             attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATgg           <  2:2973946/89‑1 (MQ=255)
                                                                              ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  1:399005/4‑93 (MQ=255)
                                                                              ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  2:1534226/4‑93 (MQ=255)
                                                                              ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  1:2142012/4‑93 (MQ=255)
                                                                                    cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    <  1:2254988/93‑1 (MQ=255)
                                                                                      tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:3749850/1‑93 (MQ=255)
                                                                                      tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:559941/1‑93 (MQ=255)
                                                                                      tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:625947/1‑93 (MQ=255)
                                                                                           |                                                                                        
TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979455‑3979633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979456‑3979639
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ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                            >  SRR4302535.2735284/1‑94 (MQ=60)
ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                            <  SRR4302535.4342005/94‑1 (MQ=60)
 CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           <  SRR4302535.1194504/94‑1 (MQ=60)
    GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                                        <  SRR4302535.3904288/94‑1 (MQ=60)
     CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       <  SRR4302535.4383649/94‑1 (MQ=60)
     CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       >  SRR4302535.1306799/1‑94 (MQ=60)
      GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      <  SRR4302535.1986445/94‑1 (MQ=60)
      GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      >  SRR4302535.2827496/1‑94 (MQ=60)
       GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                     <  SRR4302535.922070/94‑1 (MQ=60)
         AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATT                                                                                   >  SRR4302535.4480564/1‑94 (MQ=60)
          ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  <  SRR4302535.3102577/94‑1 (MQ=60)
          ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  <  SRR4302535.3093411/94‑1 (MQ=60)
          ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                  >  SRR4302535.3947870/1‑94 (MQ=60)
           TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCC                                                                                 <  SRR4302535.471048/94‑1 (MQ=60)
            GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                                >  SRR4302535.3156757/1‑94 (MQ=60)
             GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATCGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               <  SRR4302535.3317979/94‑1 (MQ=60)
              CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302535.3495158/1‑94 (MQ=60)
              CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302535.2299135/1‑94 (MQ=60)
               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302535.2273420/1‑94 (MQ=60)
               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302535.2273247/1‑94 (MQ=60)
               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302535.2497900/94‑1 (MQ=60)
               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGCGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302535.2583789/1‑94 (MQ=60)
                AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                            >  SRR4302535.4447915/1‑94 (MQ=60)
                 AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302535.3988004/94‑1 (MQ=60)
                 AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302535.2666464/94‑1 (MQ=60)
                  AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302535.1948941/94‑1 (MQ=60)
                   agTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGC                                                                         <  SRR4302535.2300430/92‑1 (MQ=60)
                     TTCCCGTCACGGCGTACCGGTTGTGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302535.2486074/94‑1 (MQ=60)
                     TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302535.662977/94‑1 (MQ=60)
                     TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302535.4546489/94‑1 (MQ=60)
                      TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      >  SRR4302535.2493822/1‑94 (MQ=60)
                      TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302535.3765982/94‑1 (MQ=60)
                      TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302535.4542590/94‑1 (MQ=60)
                         CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTTTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302535.3500345/1‑94 (MQ=60)
                          GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  <  SRR4302535.2953457/94‑1 (MQ=60)
                          GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  >  SRR4302535.1058896/1‑94 (MQ=60)
                            CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                                >  SRR4302535.863343/1‑94 (MQ=60)
                            CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                                >  SRR4302535.1716816/1‑94 (MQ=60)
                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302535.1414173/94‑1 (MQ=60)
                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302535.3585611/94‑1 (MQ=60)
                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302535.4193754/94‑1 (MQ=60)
                                 CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302535.2545194/1‑94 (MQ=60)
                                 CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302535.3590815/1‑94 (MQ=60)
                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302535.2375874/1‑94 (MQ=60)
                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302535.2466875/94‑1 (MQ=60)
                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302535.2605365/94‑1 (MQ=60)
                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302535.2336118/94‑1 (MQ=60)
                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302535.4441883/1‑94 (MQ=60)
                                   TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302535.1283582/1‑94 (MQ=60)
                                     CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       >  SRR4302535.3373089/1‑94 (MQ=60)
                                     CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       >  SRR4302535.2865890/1‑94 (MQ=60)
                                      CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      >  SRR4302535.2927572/1‑94 (MQ=60)
                                       GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     <  SRR4302535.244086/94‑1 (MQ=60)
                                        GTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCAGCAGAGTGTGTTTGTCTACGTCT                                                    >  SRR4302535.612697/1‑94 (MQ=60)
                                          TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302535.2183539/94‑1 (MQ=60)
                                           GCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACA                                                 <  SRR4302535.3714943/94‑1 (MQ=60)
                                            CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302535.1188051/1‑94 (MQ=60)
                                            CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302535.1837888/94‑1 (MQ=60)
                                            CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAGGCAT‑GAACTACATCATTCCCAATACGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302535.895295/1‑94 (MQ=60)
                                             GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302535.1935188/94‑1 (MQ=60)
                                              GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302535.1079233/1‑94 (MQ=60)
                                                TGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                            <  SRR4302535.2222184/94‑1 (MQ=60)
                                                    GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        <  SRR4302535.1965920/94‑1 (MQ=60)
                                                     CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302535.781781/1‑94 (MQ=60)
                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302535.3943166/1‑94 (MQ=60)
                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302535.3592338/1‑94 (MQ=60)
                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302535.87918/1‑94 (MQ=60)
                                                      AATATCTATTTCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      <  SRR4302535.1596074/94‑1 (MQ=60)
                                                       GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302535.3287334/1‑94 (MQ=60)
                                                       GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302535.2279232/1‑94 (MQ=60)
                                                        TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302535.2435410/1‑94 (MQ=60)
                                                        TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302535.3443853/1‑94 (MQ=60)
                                                         ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   <  SRR4302535.4146716/94‑1 (MQ=60)
                                                         ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   >  SRR4302535.2514809/1‑94 (MQ=60)
                                                          TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                                  >  SRR4302535.4488696/1‑94 (MQ=60)
                                                          TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                                  <  SRR4302535.1833445/94‑1 (MQ=60)
                                                           CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302535.1341609/94‑1 (MQ=60)
                                                            TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302535.4654075/1‑94 (MQ=60)
                                                            TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302535.1478649/1‑94 (MQ=60)
                                                            TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302535.917395/1‑94 (MQ=60)
                                                            TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302535.1142730/94‑1 (MQ=60)
                                                             ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302535.1813135/1‑94 (MQ=60)
                                                             ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               <  SRR4302535.875852/94‑1 (MQ=60)
                                                             ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               <  SRR4302535.1011211/94‑1 (MQ=60)
                                                              TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              <  SRR4302535.841441/94‑1 (MQ=60)
                                                                GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302535.3542820/1‑94 (MQ=60)
                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGTT                           <  SRR4302535.4455117/94‑1 (MQ=60)
                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302535.3603428/1‑94 (MQ=60)
                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302535.4514442/94‑1 (MQ=60)
                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302535.1378373/1‑94 (MQ=60)
                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302535.1631442/1‑94 (MQ=60)
                                                                  TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302535.1176439/1‑94 (MQ=60)
                                                                  TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302535.4365257/1‑94 (MQ=60)
                                                                  TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTACGGGGATG                          >  SRR4302535.394937/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302535.164046/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302535.610257/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302535.357086/94‑1 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302535.3381997/94‑1 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302535.3978636/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302535.3635500/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302535.2677557/1‑94 (MQ=60)
                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302535.2939945/94‑1 (MQ=60)
                                                                    TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302535.4172107/94‑1 (MQ=60)
                                                                    TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302535.3041571/94‑1 (MQ=60)
                                                                     TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       <  SRR4302535.1681103/94‑1 (MQ=60)
                                                                     TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       <  SRR4302535.1124833/94‑1 (MQ=60)
                                                                     TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       <  SRR4302535.649888/94‑1 (MQ=60)
                                                                      CTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC                      <  SRR4302535.3221603/94‑1 (MQ=60)
                                                                       TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     <  SRR4302535.1833374/94‑1 (MQ=60)
                                                                        GCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                    >  SRR4302535.701130/1‑94 (MQ=60)
                                                                         CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   <  SRR4302535.1225674/94‑1 (MQ=60)
                                                                          TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302535.2569937/94‑1 (MQ=60)
                                                                          TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302535.4332318/94‑1 (MQ=60)
                                                                           AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 >  SRR4302535.3001306/1‑94 (MQ=60)
                                                                           AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 >  SRR4302535.447453/1‑94 (MQ=60)
                                                                            ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302535.978096/1‑94 (MQ=60)
                                                                            ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302535.1576724/1‑94 (MQ=60)
                                                                            ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302535.401801/1‑94 (MQ=60)
                                                                            ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302535.2157425/1‑94 (MQ=60)
                                                                            ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                <  SRR4302535.3131711/94‑1 (MQ=60)
                                                                              G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302535.4012511/1‑94 (MQ=60)
                                                                                 CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCGACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302535.3061142/1‑94 (MQ=60)
                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         <  SRR4302535.2271042/94‑1 (MQ=60)
                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302535.1049876/1‑94 (MQ=60)
                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         <  SRR4302535.3915643/94‑1 (MQ=60)
                                                                                    ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        >  SRR4302535.797961/1‑94 (MQ=60)
                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACTTCTACAGTGCCAGCGTGCTTCCTGGGATGGTGCCATGGTTTGTGATATTG     <  SRR4302535.1092294/94‑1 (MQ=60)
                                                                                         ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    >  SRR4302535.4117172/3‑94 (MQ=60)
                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTTTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   >  SRR4302535.943052/1‑94 (MQ=60)
                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   <  SRR4302535.1782373/94‑1 (MQ=60)
                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   >  SRR4302535.4116070/1‑94 (MQ=60)
                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   <  SRR4302535.1333214/94‑1 (MQ=60)
                                                                                           GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302535.4129615/94‑1 (MQ=60)
                                                                                           GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302535.865386/94‑1 (MQ=60)
                                                                                           |                                                                                             
ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979456‑3979639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑