Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,987,807	A→G	I198I (ATT→ATC)	hemC ←	porphobilinogen deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,987,807	0	A	G	99.6%	743.2 / ‑5.0	233	I198I (ATT→ATC)	hemC	porphobilinogen deaminase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (113/119); total (114/119)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 245 total aligned reads displayed.

GTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC  >  NC_000913/3987717‑3987896

gtgATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAt                                                                                         >  1:3687834/1‑93 (MQ=255)

 tgATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtc                                                                                        <  1:834310/93‑1 (MQ=255)

 tgATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtc                                                                                        <  1:2525171/93‑1 (MQ=255)

 tgATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAAtc                                                                                        >  2:479190/1‑93 (MQ=255)

 tgAGTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtc                                                                                        <  2:4610725/93‑1 (MQ=255)

  gATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtct                                                                                       <  1:3139254/93‑1 (MQ=255)

   aTTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtctc                                                                                      <  1:1591890/93‑1 (MQ=255)

   aTTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtctc                                                                                      <  2:4378571/93‑1 (MQ=255)

   aTTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAtctc                                                                                      <  2:3437085/93‑1 (MQ=255)

    ttCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCg                                                                                     <  1:1376133/93‑1 (MQ=255)

    ttCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCg                                                                                     >  2:1868502/1‑93 (MQ=255)

      cAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCggg                                                                                   >  1:4306254/1‑93 (MQ=255)

        gcgcGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTg                                                                                 <  1:2365761/93‑1 (MQ=255)

         cgcgGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTgg                                                                                <  2:3938610/93‑1 (MQ=255)

          gcgGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGc                                                                               <  1:3523433/93‑1 (MQ=255)

           cgGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCa                                                                              <  2:2522485/93‑1 (MQ=255)

            ggCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaa                                                                             >  1:3313691/1‑93 (MQ=255)

             gCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaac                                                                            <  2:2409584/93‑2 (MQ=255)

             gCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaac                                                                            <  1:3165238/93‑2 (MQ=255)

             gCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaac                                                                            >  2:1198335/1‑92 (MQ=255)

             gCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaac                                                                            >  2:3532626/1‑92 (MQ=255)

             gCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaac                                                                            >  2:399360/1‑92 (MQ=255)

              cAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGTTGGCaacg                                                                           >  1:678612/1‑91 (MQ=255)

              cAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaacg                                                                           <  1:1186927/93‑3 (MQ=255)

                agcagcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaacgcg                                                                         >  2:4167564/1‑89 (MQ=255)

                agcagcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaacgcg                                                                         >  1:3425490/1‑89 (MQ=255)

                agcagcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCAACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCaacgcg                                                                         >  1:2065644/1‑89 (MQ=255)

                   agcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACgcggcg                                                                      >  1:3612962/1‑93 (MQ=255)

                     cTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGcgcg                                                                    >  2:2684396/1‑93 (MQ=255)

                       cgcgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGaa                                                                  <  1:4633193/93‑1 (MQ=255)

                       cgcgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGaa                                                                  <  2:272580/93‑1 (MQ=255)

                        gcgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAAt                                                                 >  1:1967635/1‑93 (MQ=255)

                         cgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACTGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATa                                                                <  1:2933575/93‑1 (MQ=255)

                         cgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATa                                                                <  2:3716315/93‑1 (MQ=255)

                         cgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATa                                                                <  2:1599336/93‑1 (MQ=255)

                            gTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGt                                                             >  2:305578/1‑93 (MQ=255)

                            gTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGt                                                             <  1:2898763/93‑1 (MQ=255)

                              gCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGa                                                           >  1:535816/1‑93 (MQ=255)

                              gCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGa                                                           >  1:1321600/1‑93 (MQ=255)

                                gTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACt                                                         <  1:1941813/93‑1 (MQ=255)

                                 tGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTc                                                        <  1:2150203/93‑1 (MQ=255)

                                 tGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTc                                                        >  2:1159645/1‑93 (MQ=255)

                                 tGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTc                                                        >  2:583295/1‑93 (MQ=255)

                                  gAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTcc                                                       >  2:3088164/1‑93 (MQ=255)

                                   aaTCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCa                                                      <  2:1064010/93‑1 (MQ=255)

                                    atcatcAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAg                                                     >  1:1877212/1‑93 (MQ=255)

                                     tcatcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGa                                                    >  2:1429028/1‑93 (MQ=255)

                                      catcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGAc                                                   <  2:744978/93‑1 (MQ=255)

                                        tcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCt                                                 >  2:1767503/1‑93 (MQ=255)

                                        tcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCt                                                 >  2:23124/1‑93 (MQ=255)

                                           aGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTaaa                                              <  2:2553756/93‑1 (MQ=255)

                                           aGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTaaa                                              >  1:4052758/1‑93 (MQ=255)

                                               ggcATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGtt                                          >  2:102369/1‑93 (MQ=255)

                                                 cATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGtttt                                        <  1:4539468/93‑1 (MQ=255)

                                                 cATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGtttt                                        <  1:1761561/93‑1 (MQ=255)

                                                  aTTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTa                                       >  2:1331429/1‑93 (MQ=255)

                                                  aTTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTa                                       >  2:1367958/1‑93 (MQ=255)

                                                   ttCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAg                                      >  2:2878939/1‑93 (MQ=255)

                                                   ttCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAg                                      >  1:1037572/1‑93 (MQ=255)

                                                     taaTACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGGCCTAAACGTTTTAggc                                    <  2:2617663/92‑3 (MQ=255)

                                                     cAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTc                                    <  2:3853663/93‑1 (MQ=255)

                                                     cAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTc                                    <  1:1907422/93‑1 (MQ=255)

                                                       aTACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCg                                  >  2:2181212/1‑93 (MQ=255)

                                                         ccccACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGc                                <  2:605870/92‑1 (MQ=255)

                                                         aCCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGc                                >  1:2277310/1‑93 (MQ=255)

                                                         aCCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGc                                <  2:2063874/93‑1 (MQ=255)

                                                         aCCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGc                                <  2:2964710/93‑1 (MQ=255)

                                                           ccACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                              <  1:122346/93‑1 (MQ=255)

                                                           ccACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                              >  1:1084559/1‑93 (MQ=255)

                                                            cACCGCGCCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAc                             <  2:4151686/93‑1 (MQ=255)

                                                            cACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAc                             <  2:4014112/93‑1 (MQ=255)

                                                                gCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCa                         >  2:3249279/1‑93 (MQ=255)

                                                                  aCCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                       >  2:1808785/1‑93 (MQ=255)

                                                                    cTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                     >  1:3751967/1‑93 (MQ=255)

                                                                    cTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                     <  1:4497590/93‑1 (MQ=255)

                                                                    cTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                     >  2:3344342/1‑93 (MQ=255)

                                                                    cTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                     <  1:2084516/93‑1 (MQ=255)

                                                                     ttGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAat                    <  2:4438164/93‑1 (MQ=255)

                                                                     ttGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAat                    <  2:719684/93‑1 (MQ=255)

                                                                      tGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCATCGCGGCGCGATTACGTGACTCCAGCCCTAAACGTTTTAGTCCGGCTACGGCAAGAatg                   <  2:1126109/93‑1 (MQ=255)

                                                                      tGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatg                   <  1:2972426/93‑1 (MQ=255)

                                                                       gTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatga                  <  1:33507/93‑1 (MQ=255)

                                                                       gTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatga                  <  1:1029154/93‑1 (MQ=255)

                                                                       gTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatga                  >  1:924257/1‑93 (MQ=255)

                                                                        tCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatgat                 <  1:553101/93‑1 (MQ=255)

                                                                         ccTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatgatg                <  2:3546541/93‑1 (MQ=255)

                                                                          cTACCGCCGGAAGAGAGATCTCGGGGGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATgg               >  2:4481300/1‑93 (MQ=255)

                                                                           tACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGc              >  1:2560870/1‑93 (MQ=255)

                                                                           tACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGc              >  1:4142576/1‑93 (MQ=255)

                                                                             ccgccgGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAt            <  1:3046037/93‑1 (MQ=255)

                                                                              cgccgGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATc           >  2:3443295/1‑93 (MQ=255)

                                                                                 cgGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTa        <  2:1662811/93‑1 (MQ=255)

                                                                                   gAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTAtt      <  2:4027748/93‑1 (MQ=255)

                                                                                   gAAGAGAGATCTCAGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTAtt      <  1:2677619/93‑1 (MQ=255)

                                                                                    aaGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc     <  1:1671246/93‑1 (MQ=255)

                                                                                    aaGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc     <  1:4214912/93‑1 (MQ=255)

                                                                                    aaGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc     <  2:1412878/93‑1 (MQ=255)

                                                                                    aaGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc     <  1:598433/93‑1 (MQ=255)

                                                                                    aaGAGAGATCTAGGGTGGCAACGCGGCGCGAATACGTGAATCCAGACCTAAACGTTTTAGTCCGGCCACGGCAAGAATGATCGCATCGTATTc     >  2:3653089/1‑93 (MQ=255)

                                                                                       agagATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGcc  >  1:2430843/1‑93 (MQ=255)

GTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC  >  NC_000913/3987717‑3987896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTT  >  NC_000913/3987714‑3987899

GTGGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAG                                                                                              >  SRR4302535.369140/1‑94 (MQ=60)

 TGGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGA                                                                                             >  SRR4302535.1599325/1‑94 (MQ=60)

 TGGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGA                                                                                             >  SRR4302535.3853205/1‑94 (MQ=60)

 TCGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGA                                                                                             <  SRR4302535.2726341/94‑1 (MQ=60)

  GGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAT                                                                                            >  SRR4302535.3713351/1‑94 (MQ=60)

  GGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGAT                                                                                            <  SRR4302535.2783795/94‑1 (MQ=60)

   GTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATC                                                                                           <  SRR4302535.2956777/94‑1 (MQ=60)

    TGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCT                                                                                          <  SRR4302535.840160/94‑1 (MQ=60)

    TGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCT                                                                                          >  SRR4302535.2763813/1‑94 (MQ=60)

    TGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCT                                                                                          <  SRR4302535.2543367/94‑1 (MQ=60)

     GATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTC                                                                                         <  SRR4302535.3183129/94‑1 (MQ=60)

     GATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTC                                                                                         <  SRR4302535.3161098/94‑1 (MQ=60)

      ATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCG                                                                                        <  SRR4302535.2232212/94‑1 (MQ=60)

      ATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCG                                                                                        <  SRR4302535.1603375/94‑1 (MQ=60)

       TTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGG                                                                                       >  SRR4302535.3640802/1‑94 (MQ=60)

       TTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGG                                                                                       <  SRR4302535.1385959/94‑1 (MQ=60)

        TCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGG                                                                                      <  SRR4302535.571342/94‑1 (MQ=60)

        TCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGG                                                                                      >  SRR4302535.4335717/1‑94 (MQ=60)

         CAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGG                                                                                     >  SRR4302535.1265847/1‑94 (MQ=60)

           GCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGG                                                                                   <  SRR4302535.2382744/94‑1 (MQ=60)

            CGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACATTGTCCTACCGCCGGAAGAGAGATCTCGTGTGGC                                                                                  >  SRR4302535.594640/1‑94 (MQ=60)

             GCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCA                                                                                 <  SRR4302535.3547902/94‑1 (MQ=60)

              CGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAA                                                                                >  SRR4302535.3336749/1‑94 (MQ=60)

              CGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAA                                                                                <  SRR4302535.4569477/94‑1 (MQ=60)

               GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAC                                                                               >  SRR4302535.2609005/1‑94 (MQ=60)

                GCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGTTGGCAAcg                                                                              >  SRR4302535.683358/1‑92 (MQ=60)

                GCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcg                                                                              <  SRR4302535.3187180/94‑3 (MQ=60)

                 CAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgc                                                                             <  SRR4302535.1195409/94‑4 (MQ=60)

                 CAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgc                                                                             <  SRR4302535.2158884/94‑4 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            >  SRR4302535.3449309/1‑90 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            <  SRR4302535.2467436/94‑5 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            >  SRR4302535.492065/1‑90 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            <  SRR4302535.200440/94‑5 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            >  SRR4302535.2991603/1‑90 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACAGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            >  SRR4302535.333890/1‑90 (MQ=60)

                  AAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCAACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAAcgcg                                                                            >  SRR4302535.2080534/1‑90 (MQ=60)

                    GCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGtcaacgcgac                                                                          >  SRR4302535.230614/1‑84 (MQ=60)

                     CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCG                                                                         <  SRR4302535.1258956/94‑1 (MQ=60)

                     CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCG                                                                         >  SRR4302535.3638014/1‑94 (MQ=60)

                          CGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAAT                                                                    >  SRR4302535.1981815/1‑94 (MQ=60)

                          CGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAAT                                                                    <  SRR4302535.4664626/94‑1 (MQ=60)

                            CGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACTGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATAC                                                                  <  SRR4302535.2954223/94‑1 (MQ=60)

                             GAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACG                                                                 >  SRR4302535.1677422/1‑94 (MQ=60)

                             GAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACG                                                                 >  SRR4302535.2387621/1‑94 (MQ=60)

                               GTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTG                                                               <  SRR4302535.2919261/94‑1 (MQ=60)

                                TGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGA                                                              >  SRR4302535.539557/1‑94 (MQ=60)

                                TGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGA                                                              >  SRR4302535.1331035/1‑94 (MQ=60)

                                  CGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACT                                                            <  SRR4302535.4229908/94‑1 (MQ=60)

                                  CGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACT                                                            <  SRR4302535.1304785/94‑1 (MQ=60)

                                   GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                           >  SRR4302535.4169137/1‑94 (MQ=60)

                                   GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                           <  SRR4302535.1955799/94‑1 (MQ=60)

                                   GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                           >  SRR4302535.1915278/1‑94 (MQ=60)

                                    TGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCC                                                          <  SRR4302535.2165665/94‑1 (MQ=60)

                                     GAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCA                                                         >  SRR4302535.1481229/1‑94 (MQ=60)

                                      AATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAG                                                        <  SRR4302535.3056924/94‑1 (MQ=60)

                                      AATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAG                                                        >  SRR4302535.1890735/1‑94 (MQ=60)

                                        TCATCAAGGCGGCATTCAATACCAACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGAC                                                      <  SRR4302535.1339600/94‑1 (MQ=60)

                                         CATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTCCCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGACTACGTGACTCCAGACC                                                     >  SRR4302535.3817798/1‑94 (MQ=60)

                                          ATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCT                                                    <  SRR4302535.707880/94‑1 (MQ=60)

                                          ATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCT                                                    >  SRR4302535.49191/1‑94 (MQ=60)

                                            CAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAA                                                  >  SRR4302535.4592036/1‑94 (MQ=60)

                                             AAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAA                                                 >  SRR4302535.4080507/1‑94 (MQ=60)

                                              AGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAAC                                                <  SRR4302535.1940832/94‑1 (MQ=60)

                                                 CGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTT                                             >  SRR4302535.1718888/1‑94 (MQ=60)

                                                 CGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTT                                             <  SRR4302535.2357960/94‑1 (MQ=60)

                                                 CGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTT                                             <  SRR4302535.2348763/94‑1 (MQ=60)

                                                   GCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTT                                           <  SRR4302535.3318818/94‑1 (MQ=60)

                                                   GCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTT                                           >  SRR4302535.3317677/1‑94 (MQ=60)

                                                    CATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTA                                          <  SRR4302535.4570517/94‑1 (MQ=60)

                                                    CATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTA                                          <  SRR4302535.1774321/94‑1 (MQ=60)

                                                     ATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAG                                         >  SRR4302535.1044940/1‑94 (MQ=60)

                                                     ATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAG                                         >  SRR4302535.1224782/1‑94 (MQ=60)

                                                      TTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGT                                        <  SRR4302535.1716895/94‑1 (MQ=60)

                                                      TTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGT                                        <  SRR4302535.2105447/94‑1 (MQ=60)

                                                      TTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGT                                        <  SRR4302535.3393320/94‑1 (MQ=60)

                                                        CAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                      <  SRR4302535.1921173/94‑1 (MQ=60)

                                                          ATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGG                                    >  SRR4302535.3092684/1‑94 (MQ=60)

                                                          ATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACCTTTTAGTCCTG                                    >  SRR4302535.1080718/1‑94 (MQ=60)

                                                           TACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGC                                   >  SRR4302535.2293572/1‑94 (MQ=60)

                                                           TACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGC                                   <  SRR4302535.1133158/94‑1 (MQ=60)

                                                            ACCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCT                                  <  SRR4302535.142519/94‑1 (MQ=60)

                                                             CCCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTA                                 >  SRR4302535.1092270/1‑94 (MQ=60)

                                                              CCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAC                                <  SRR4302535.4339802/94‑1 (MQ=60)

                                                              CCACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAC                                <  SRR4302535.123207/94‑1 (MQ=60)

                                                               CACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                               <  SRR4302535.2009738/94‑1 (MQ=60)

                                                               CACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                               <  SRR4302535.3499278/94‑1 (MQ=60)

                                                                ACCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGG                              >  SRR4302535.1178193/1‑94 (MQ=60)

                                                                 CCGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGC                             >  SRR4302535.2904057/1‑94 (MQ=60)

                                                                  CGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCA                            >  SRR4302535.826765/1‑94 (MQ=60)

                                                                  CGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCA                            <  SRR4302535.1071864/94‑1 (MQ=60)

                                                                  CGCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGCTTTAGTCCGGCTACGGCA                            >  SRR4302535.2413355/1‑94 (MQ=60)

                                                                   GCACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAA                           >  SRR4302535.1941419/1‑94 (MQ=60)

                                                                    CACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCACG                          >  SRR4302535.539819/1‑94 (MQ=60)

                                                                    CACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                          >  SRR4302535.357458/1‑94 (MQ=60)

                                                                    CACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                          >  SRR4302535.2522928/1‑94 (MQ=60)

                                                                    CACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                          >  SRR4302535.3709824/1‑94 (MQ=60)

                                                                     ACCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGA                         >  SRR4302535.2919448/1‑94 (MQ=60)

                                                                      CCTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAA                        >  SRR4302535.3777936/1‑94 (MQ=60)

                                                                       CTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                       <  SRR4302535.4528469/94‑1 (MQ=60)

                                                                       CTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                       >  SRR4302535.2094173/1‑94 (MQ=60)

                                                                       CTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                       <  SRR4302535.4196099/94‑1 (MQ=60)

                                                                       CTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                       <  SRR4302535.2099529/94‑1 (MQ=60)

                                                                       CTTGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                       <  SRR4302535.870730/94‑1 (MQ=60)

                                                                         TGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGA                     >  SRR4302535.930803/1‑94 (MQ=60)

                                                                         TGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGA                     <  SRR4302535.2993289/94‑1 (MQ=60)

                                                                         TGTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGA                     >  SRR4302535.4259280/1‑94 (MQ=60)

                                                                          GTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGAT                    <  SRR4302535.1036460/94‑1 (MQ=60)

                                                                          GTCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGAT                    <  SRR4302535.33765/94‑1 (MQ=60)

                                                                           TCCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATG                   <  SRR4302535.556972/94‑1 (MQ=60)

                                                                            CCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGG                  <  SRR4302535.442188/94‑1 (MQ=60)

                                                                            CCTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGG                  <  SRR4302535.3510338/94‑1 (MQ=60)

                                                                             CTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGC                 >  SRR4302535.4170942/1‑94 (MQ=60)

                                                                             CTACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGC                 >  SRR4302535.2579308/1‑94 (MQ=60)

                                                                               ACCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAT               <  SRR4302535.936270/94‑1 (MQ=60)

                                                                                CCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC              <  SRR4302535.3067373/94‑1 (MQ=60)

                                                                                CCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC              >  SRR4302535.680168/1‑94 (MQ=60)

                                                                                CCGCCGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC              <  SRR4302535.1979924/94‑1 (MQ=60)

                                                                                    CGGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTAT          <  SRR4302535.1850487/94‑1 (MQ=60)

                                                                                     GGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATT         <  SRR4302535.3073410/94‑1 (MQ=60)

                                                                                     GGAAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATT         >  SRR4302535.104045/1‑94 (MQ=60)

                                                                                      GAAGAGAGATCTCAGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTC        <  SRR4302535.2696773/94‑1 (MQ=60)

                                                                                       AAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCG       <  SRR4302535.4243758/94‑1 (MQ=60)

                                                                                       AAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCG       <  SRR4302535.602619/94‑1 (MQ=60)

                                                                                       AAGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCG       <  SRR4302535.1683345/94‑1 (MQ=60)

                                                                                        AGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGC      <  SRR4302535.3763864/94‑1 (MQ=60)

                                                                                        AGAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGC      <  SRR4302535.2550350/94‑1 (MQ=60)

                                                                                         GAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC     <  SRR4302535.3814445/94‑1 (MQ=60)

                                                                                         GAGAGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC     >  SRR4302535.2448355/1‑94 (MQ=60)

                                                                                            AGATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTT  <  SRR4302535.2099676/94‑1 (MQ=60)

                                                                                            AGATCTCGGGCGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTT  <  SRR4302535.3308699/94‑1 (MQ=60)

GTGGTGATTCAGCGCGGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTT  >  NC_000913/3987714‑3987899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`