Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,9071.G94.1% 665.9 / 33.5 221intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (5/8);  new base G (125/83);  total (130/91)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
*NC_0009134,007,9072.G94.1% 657.8 / 33.5 221intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (5/8);  new base G (125/83);  total (130/91)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 242 total aligned reads displayed.

GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996
                                                                                             ||                                                                                         
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgc                                                                                             >  1:1721227/1‑93 (MQ=255)
 ttgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCGTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcg                                                                                            >  1:2518763/1‑92 (MQ=255)
 ttgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcg                                                                                            >  2:2406498/1‑92 (MQ=255)
   gttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcggc                                                                                          >  2:4188695/1‑90 (MQ=255)
          cTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACt                                                                                   >  2:2742263/1‑93 (MQ=255)
           tGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACtt                                                                                  >  1:1432252/1‑93 (MQ=255)
           tGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACtt                                                                                  >  1:1923624/1‑93 (MQ=255)
             aTTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTtg                                                                                >  2:2192255/1‑93 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTtgt                                                                               >  2:115587/1‑93 (MQ=255)
               ttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTtgtg                                                                              <  1:133867/93‑1 (MQ=255)
                tAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTgtgt                                                                             <  1:243056/93‑1 (MQ=255)
                tAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTgtgt                                                                             <  1:2367100/93‑1 (MQ=255)
                 aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGtt                                                                            <  1:972195/93‑1 (MQ=255)
                 aGATGGCACGTTACTTTCTCACGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGtt                                                                            >  2:1921551/1‑93 (MQ=255)
                  gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGttt                                                                           <  1:1392058/93‑1 (MQ=255)
                  gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGttt                                                                           >  2:1340570/1‑93 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGc                                                                         <  1:1570957/93‑1 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGc                                                                         >  2:2599579/1‑93 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGc                                                                         <  1:2407788/93‑1 (MQ=255)
                     ggCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCg                                                                        >  1:254374/1‑93 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     >  2:1979898/1‑93 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     >  1:319668/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    >  1:1590496/1‑93 (MQ=255)
                          gTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                                   >  2:866902/1‑93 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  2:2942249/1‑93 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  <  2:3410900/93‑1 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  1:4655929/1‑93 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  2:4400705/1‑93 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  2:576396/1‑93 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 >  1:8244/1‑93 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               >  2:4120926/1‑93 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              >  1:1812409/1‑93 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              <  2:667224/93‑1 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              <  2:1385735/93‑1 (MQ=255)
                                ttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGCCGTca                                                             >  1:324537/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTGGTcacca                                                          >  1:3623648/1‑93 (MQ=255)
                                     ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                                        <  2:3463277/93‑1 (MQ=255)
                                     ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                                        >  2:249649/1‑93 (MQ=255)
                                      cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                                       >  1:4265593/1‑93 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGGCCGGTCGTCACCCCGTTGa                                                    >  1:3837890/1‑93 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  1:2314560/93‑1 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  1:1143923/93‑1 (MQ=255)
                                          cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCCGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                                   >  1:3016968/1‑93 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  >  1:764428/1‑93 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  <  1:3168579/93‑1 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  >  1:171033/1‑93 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  >  1:2261170/1‑93 (MQ=255)
                                              cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgg                                               <  2:1488415/93‑1 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              >  1:2900330/1‑93 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              >  1:3358240/1‑93 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             >  1:1781955/1‑93 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             >  1:1798499/1‑93 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             <  2:165583/93‑1 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             <  2:732984/93‑1 (MQ=255)
                                                 tATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGc                                            <  1:4561766/93‑1 (MQ=255)
                                                 tATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGc                                            <  1:4075020/93‑1 (MQ=255)
                                                  aTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa                                           >  1:1299191/1‑93 (MQ=255)
                                                   tCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaa                                          >  1:1536490/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:8398/93‑1 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         >  2:2904259/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGCGCaaa                                         >  2:4664343/1‑93 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt                                        >  2:2646030/1‑93 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt                                        >  1:4337603/1‑93 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt                                        <  2:1767365/93‑1 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAt                                        >  1:370518/1‑93 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAACCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  2:1841151/1‑93 (MQ=255)
                                                       cTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                                      >  2:251122/1‑93 (MQ=255)
                                                          aCGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg                                   >  2:3516175/1‑93 (MQ=255)
                                                           cGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa                                  <  2:3235754/93‑1 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 >  2:4282806/1‑93 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 <  1:3912274/93‑1 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                <  2:3867169/93‑1 (MQ=255)
                                                              cAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATaa                               >  1:2925087/1‑93 (MQ=255)
                                                               aaaGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt                              >  2:3716483/1‑93 (MQ=255)
                                                                  gAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATCATCTg                           >  1:3442792/1‑93 (MQ=255)
                                                                  gAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTg                           >  1:779134/1‑93 (MQ=255)
                                                                    aaCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGga                         >  2:589424/1‑93 (MQ=255)
                                                                    aaCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGga                         >  1:103692/1‑93 (MQ=255)
                                                                      ctctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTTGGGCAAATTCGCGATAATCTGgaga                       >  1:4502252/1‑93 (MQ=255)
                                                                       tctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      <  1:2075152/93‑1 (MQ=255)
                                                                       tctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      <  1:2775376/93‑1 (MQ=255)
                                                                        ctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAtt                     <  2:3420280/93‑1 (MQ=255)
                                                                         tGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    >  1:27490/1‑93 (MQ=255)
                                                                         tGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    <  2:3531811/93‑1 (MQ=255)
                                                                          gAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGTGGCAAATTCGCGATAATCTGGAGATTaa                   >  2:3317212/1‑93 (MQ=255)
                                                                          gAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTaa                   >  2:3618229/1‑93 (MQ=255)
                                                                            aGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 <  2:3119240/93‑1 (MQ=255)
                                                                            aGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 >  1:4522763/1‑93 (MQ=255)
                                                                             gctgctCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTc                >  2:3024536/1‑93 (MQ=255)
                                                                             gctgctCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTc                >  1:2018337/1‑93 (MQ=255)
                                                                              ctgctCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTGGCGATAATCTGGAGATTAAGTCt               <  2:2063194/93‑1 (MQ=255)
                                                                                  accgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         >  1:923352/5‑93 (MQ=255)
                                                                                   ccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        <  1:4229708/90‑1 (MQ=255)
                                                                                     gcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAtt      <  2:3163441/92‑1 (MQ=255)
                                                                                      cgcgcgG‑‑CATCAACTGTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGTGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTa     <  1:2225614/93‑1 (MQ=255)
                                                                                        cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  1:1082947/93‑1 (MQ=255)
                                                                                        cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  2:2823814/93‑1 (MQ=255)
                                                                                        cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAcc   <  2:1218204/93‑1 (MQ=255)
                                                                                         gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCt  >  1:266114/1‑93 (MQ=255)
                                                                                         gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCt  >  1:4457/1‑93 (MQ=255)
                                                                                             ||                                                                                         
GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000
                                                                                             |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC                                                                                                 >  SRR4302535.1733673/1‑94 (MQ=60)
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC                                                                                                 >  SRR4302535.1424446/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCGTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑G                                                                                              >  SRR4302535.2536917/1‑94 (MQ=60)
  TTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCgg                                                                                               >  SRR4302535.1863814/1‑92 (MQ=60)
  TTGGTGCGTCTGATTTAGATGGCATGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCgg                                                                                               <  SRR4302535.2295407/94‑3 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggc                                                                                              >  SRR4302535.3440735/1‑91 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggc                                                                                              >  SRR4302535.789285/1‑91 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggca                                                                                             <  SRR4302535.2857311/94‑5 (MQ=60)
      TGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATC                                                                                           >  SRR4302535.3643443/1‑94 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATAGGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAA                                                                                         >  SRR4302535.1390140/1‑94 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggaatcaa                                                                                         >  SRR4302535.2117253/1‑86 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302535.364669/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302535.158909/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302535.4551687/1‑94 (MQ=60)
          TCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACT                                                                                       <  SRR4302535.2686367/94‑1 (MQ=60)
          TCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACT                                                                                       >  SRR4302535.3938580/1‑94 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302535.2279469/1‑94 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302535.2944280/1‑94 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302535.1442477/1‑94 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302535.1937488/1‑94 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTT                                                                                     <  SRR4302535.4546990/94‑1 (MQ=60)
             GATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTG                                                                                    >  SRR4302535.840382/1‑94 (MQ=60)
              ATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGT                                                                                   <  SRR4302535.3288257/94‑1 (MQ=60)
                TTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGT                                                                                 <  SRR4302535.134802/94‑1 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                <  SRR4302535.2384093/94‑1 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                <  SRR4302535.244720/94‑1 (MQ=60)
                 TAGATGGAACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                >  SRR4302535.46649/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTT                                                                               <  SRR4302535.979092/94‑1 (MQ=60)
                  AGATGGCACGTTACTTGCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTT                                                                               <  SRR4302535.1780042/94‑1 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTG                                                                              <  SRR4302535.705858/94‑1 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTG                                                                              <  SRR4302535.1401994/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGC                                                                             >  SRR4302535.2709817/1‑94 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302535.2425119/94‑1 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            >  SRR4302535.256129/1‑94 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            >  SRR4302535.2831114/1‑94 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302535.1582298/94‑1 (MQ=60)
                      GGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGA                                                                           <  SRR4302535.2261269/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302535.3728323/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302535.3822027/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302535.3476560/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302535.4492419/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302535.1914424/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302535.844083/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302535.321878/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302535.1601971/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302535.2404864/1‑94 (MQ=60)
                           GTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      >  SRR4302535.2634980/1‑94 (MQ=60)
                           GTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      >  SRR4302535.4687437/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302535.3267750/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302535.8312/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302535.4243339/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     <  SRR4302535.471278/94‑1 (MQ=60)
                             TACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCG                                                                    >  SRR4302535.4554340/1‑94 (MQ=60)
                              ACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGT                                                                   >  SRR4302535.1316765/1‑94 (MQ=60)
                               CTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTC                                                                  >  SRR4302535.1825516/1‑94 (MQ=60)
                                TTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGCCGTCA                                                                 >  SRR4302535.326783/1‑94 (MQ=60)
                                   CTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTGGTCACCA                                                              >  SRR4302535.3648765/1‑94 (MQ=60)
                                   CTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGACGTCACCC                                                              >  SRR4302535.1383278/1‑94 (MQ=60)
                                    TCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302535.1101572/94‑1 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302535.1362380/1‑94 (MQ=60)
                                      CCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGT                                                           >  SRR4302535.4294804/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAACCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCGCGTTG                                                         <  SRR4302535.1719514/94‑1 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         <  SRR4302535.2067156/94‑1 (MQ=60)
                                         ACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGGCCGGTCGTCACCCCGTTGA                                                        >  SRR4302535.3864347/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302535.1076659/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302535.4564093/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302535.4275835/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302535.1152080/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302535.2331120/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCCGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302535.3038105/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      >  SRR4302535.769811/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      >  SRR4302535.2277278/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      >  SRR4302535.172217/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGCTCGTCACCACGTTGATG                                                      >  SRR4302535.2353684/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGCACGTCACCACGCTGGTG                                                      >  SRR4302535.1819024/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302535.3190529/94‑1 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302535.1939787/94‑1 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  >  SRR4302535.3381617/1‑94 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302535.1588276/94‑1 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  >  SRR4302535.2920833/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302535.2150626/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302535.1811503/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302535.2432124/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302535.1794849/1‑94 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               >  SRR4302535.1308455/1‑94 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               <  SRR4302535.4102901/94‑1 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               <  SRR4302535.4592899/94‑1 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              >  SRR4302535.1547560/1‑94 (MQ=60)
                                                    TCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAA                                             >  SRR4302535.1605917/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302535.373079/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302535.4367267/1‑94 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            >  SRR4302535.1497722/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTC                                          >  SRR4302535.2774181/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302535.4332788/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302535.4267927/1‑94 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAT                                     <  SRR4302535.4430881/94‑1 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    <  SRR4302535.4492438/94‑1 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    <  SRR4302535.3939056/94‑1 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   >  SRR4302535.2945709/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  >  SRR4302535.1732473/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  >  SRR4302535.2764792/1‑94 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 >  SRR4302535.2469236/1‑94 (MQ=60)
                                                                 AAGAAGCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                <  SRR4302535.3811662/94‑1 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                >  SRR4302535.1230305/1‑94 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATCATCTG                               >  SRR4302535.3466733/1‑94 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               >  SRR4302535.784605/1‑94 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              >  SRR4302535.770038/1‑94 (MQ=60)
                                                                    AAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGA                             >  SRR4302535.104426/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302535.2552175/94‑1 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            >  SRR4302535.1799667/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302535.976995/94‑1 (MQ=60)
                                                                      ACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTTGGGCAAATTCGCGATAATCTGGAGA                           >  SRR4302535.4533169/1‑94 (MQ=60)
                                                                        TCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATT                         <  SRR4302535.2795137/94‑1 (MQ=60)
                                                                        TCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATT                         >  SRR4302535.1177421/1‑94 (MQ=60)
                                                                        TCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATT                         <  SRR4302535.2090100/94‑1 (MQ=60)
                                                                         CTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA                        >  SRR4302535.3188195/1‑94 (MQ=60)
                                                                         CTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA                        >  SRR4302535.27708/1‑94 (MQ=60)
                                                                           GAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAG                      <  SRR4302535.2520315/94‑1 (MQ=60)
                                                                            AAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302535.3400631/1‑94 (MQ=60)
                                                                            AAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302535.4553753/1‑94 (MQ=60)
                                                                            AAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302535.2075238/1‑94 (MQ=60)
                                                                             AGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302535.1547694/1‑94 (MQ=60)
                                                                             AGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302535.2032881/1‑94 (MQ=60)
                                                                             AGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302535.3199446/1‑94 (MQ=60)
                                                                               CTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302535.3945939/1‑94 (MQ=60)
                                                                                TGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTA                 <  SRR4302535.1750308/94‑1 (MQ=60)
                                                                                 GCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                <  SRR4302535.1549999/94‑1 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302535.2074209/1‑94 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302535.2613872/1‑94 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302535.2057974/1‑94 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302535.1751907/1‑94 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGAGATAATCTGGAGATTAAGTCTTACA               <  SRR4302535.1796948/94‑1 (MQ=60)
                                                                                   TCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302535.518676/94‑1 (MQ=60)
                                                                                    CACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             <  SRR4302535.1790690/94‑1 (MQ=60)
                                                                                    CACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302535.929894/1‑94 (MQ=60)
                                                                                    CACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             <  SRR4302535.1540498/94‑1 (MQ=60)
                                                                                      GCGCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302535.2963811/94‑1 (MQ=60)
                                                                                      CCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           <  SRR4302535.4258655/94‑1 (MQ=60)
                                                                                      CCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           <  SRR4302535.3993035/94‑1 (MQ=60)
                                                                                       CGCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302535.2990299/94‑1 (MQ=60)
                                                                                       CGCGCGG‑‑CATCAACTGTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGTGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302535.2241449/94‑1 (MQ=60)
                                                                                       CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATT          >  SRR4302535.3235649/1‑94 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      >  SRR4302535.267962/1‑94 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      >  SRR4302535.4498/1‑94 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      <  SRR4302535.2384532/94‑1 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      <  SRR4302535.1090644/94‑1 (MQ=60)
                                                                                            GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   <  SRR4302535.1726930/94‑1 (MQ=60)
                                                                                             G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  <  SRR4302535.1083887/94‑1 (MQ=60)
                                                                                             G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  SRR4302535.3418210/1‑94 (MQ=60)
                                                                                             |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑