Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,9071.G92.0% 1063.6 / 85.8 363intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (8/21);  new base G (179/153);  total (188/175)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01
*NC_0009134,007,9072.G92.0% 1051.6 / 85.8 363intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (8/21);  new base G (180/152);  total (188/175)
Fisher's exact test for biased strand distribution p-value = 6.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 406 total aligned reads displayed.

GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996
                                                                                               ||                                                                                         
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgc                                                                                             >  1:5811177/1‑93 (MQ=255)
gttgttGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgc                                                                                             >  2:1372485/1‑93 (MQ=255)
 ttgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcg                                                                                            >  1:7740754/1‑92 (MQ=255)
 ttgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcg                                                                                            <  2:2156028/93‑2 (MQ=255)
  tgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcgg                                                                                           >  1:4636122/1‑91 (MQ=255)
  tgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcgg                                                                                           >  2:3127180/1‑91 (MQ=255)
   gttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcggc                                                                                          >  2:149315/1‑90 (MQ=255)
   gttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcggc                                                                                          >  2:4992364/1‑90 (MQ=255)
    ttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑gcgcgcggca                                                                                         <  2:6239479/93‑5 (MQ=255)
           tGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACtt                                                                                  <  1:2551115/93‑1 (MQ=255)
            gATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACttt                                                                                 >  1:1566620/1‑93 (MQ=255)
            gATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACttt                                                                                 >  2:3833345/1‑93 (MQ=255)
             aTTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTtg                                                                                >  2:1088504/1‑93 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTtgt                                                                               >  2:5869611/1‑93 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTtgt                                                                               >  2:2292434/1‑93 (MQ=255)
               ttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTtgtg                                                                              >  2:2553653/1‑93 (MQ=255)
                 aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGtt                                                                            <  1:3993307/93‑1 (MQ=255)
                    tgtcACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAATAAACTCTGAAGCTGCTGACC‑‑GCGCGCGGCATCAACTTTGTCCTTGc                                                                         >  2:6760973/4‑93 (MQ=255)
                      gCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGa                                                                       <  2:5310494/93‑1 (MQ=255)
                       cACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGAc                                                                      >  1:7631314/1‑93 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     >  1:4297375/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCTCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    <  1:4908404/93‑1 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    >  1:4422845/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    <  2:2877792/93‑1 (MQ=255)
                          gTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                                   >  2:5193617/1‑93 (MQ=255)
                           ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  >  2:3980476/1‑93 (MQ=255)
                           ttACTTCCTCCCGACCAAACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  <  2:5519271/93‑1 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCTCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 >  2:7028716/1‑93 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               <  2:7767711/93‑1 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              >  2:772347/1‑93 (MQ=255)
                                 tctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcac                                                            >  2:328008/1‑93 (MQ=255)
                                 tctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcac                                                            >  2:1737714/1‑93 (MQ=255)
                                  ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacc                                                           >  2:2330889/1‑93 (MQ=255)
                                  ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacc                                                           >  1:1327617/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          <  1:3412540/93‑1 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          >  1:2829260/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCACCTTACGCCAAAGAAACTCTGAAGCAACTAACC‑‑GCGCGCAACATCAAAAAAGTGTTTGCAACCGGTCGTcacca                                                          <  2:7243593/93‑1 (MQ=255)
                                   ccccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          <  1:7073589/92‑1 (MQ=255)
                                     ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                                        <  2:178779/93‑1 (MQ=255)
                                      cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                                       <  1:352636/93‑1 (MQ=255)
                                      cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                                       >  1:527696/1‑93 (MQ=255)
                                       gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt                                                      <  1:4239098/93‑1 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  2:6532634/93‑1 (MQ=255)
                                          cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                                   >  1:4585609/1‑93 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGTGCGCGG‑‑CATTAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 <  1:6387172/93‑1 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 >  2:1598729/1‑93 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 <  1:17594/93‑1 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgg                                                 >  1:716918/1‑92 (MQ=255)
                                             aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                                                <  1:2025620/93‑1 (MQ=255)
                                             aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                                                <  1:6785052/93‑1 (MQ=255)
                                             aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                                                <  1:6482503/93‑1 (MQ=255)
                                              cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgg                                               <  1:6198000/93‑1 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              >  2:1148797/1‑93 (MQ=255)
                                               cttATCCCCCTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              <  1:1860702/92‑1 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             >  1:2416596/1‑93 (MQ=255)
                                                  aTCCCCTTACGCCAAAGAAACTCTGAATCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa                                           <  1:7668963/93‑1 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:7532522/93‑1 (MQ=255)
                                                      ctttACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       <  2:7437710/91‑1 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  1:3047116/1‑93 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  2:358744/1‑93 (MQ=255)
                                                       cTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                                      >  1:1001671/1‑93 (MQ=255)
                                                       cTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                                      >  1:6675451/1‑93 (MQ=255)
                                                          aCGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg                                   >  2:4852883/1‑93 (MQ=255)
                                                           cGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa                                  <  1:7348329/93‑1 (MQ=255)
                                                           cGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa                                  <  1:6963509/93‑1 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 >  2:2985876/1‑93 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                <  2:5445009/93‑1 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                >  1:7319086/1‑93 (MQ=255)
                                                              cAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATac                               >  2:3370487/1‑92 (MQ=255)
                                                               aaaGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt                              >  2:7151503/1‑93 (MQ=255)
                                                               aaaGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt                              >  1:452360/1‑93 (MQ=255)
                                                                   aaaCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGGTGTGGGGCAAATTCGCGATCATCTgg                          >  1:469421/1‑93 (MQ=255)
                                                                    aaCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTCGCGATAATCTGga                         >  2:7934631/1‑93 (MQ=255)
                                                                    aaCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGga                         <  1:4367854/93‑1 (MQ=255)
                                                                     aCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgag                        >  1:815224/1‑93 (MQ=255)
                                                                       tctGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      >  1:5042827/1‑93 (MQ=255)
                                                                       tctGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      >  1:5979722/1‑93 (MQ=255)
                                                                        ctGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAtt                     <  2:4592392/93‑1 (MQ=255)
                                                                         tGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    <  1:5411367/93‑1 (MQ=255)
                                                                         tGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    <  1:7568998/93‑1 (MQ=255)
                                                                          gAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTaa                   >  1:5608898/1‑93 (MQ=255)
                                                                          gAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTaa                   <  2:6917084/93‑1 (MQ=255)
                                                                           aaGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAg                  >  1:3883251/1‑93 (MQ=255)
                                                                           aaGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAg                  >  2:4002189/1‑93 (MQ=255)
                                                                             gctgctCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTc                <  1:3298344/93‑1 (MQ=255)
                                                                               tgctCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCtt              >  2:598236/1‑93 (MQ=255)
                                                                                gctCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTa             <  1:5845904/93‑1 (MQ=255)
                                                                                gctCACC‑‑GCGCGCGGCATCAACTTCGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTa             <  2:5719786/93‑1 (MQ=255)
                                                                                 ctCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAc            >  1:7451805/1‑93 (MQ=255)
                                                                                  cccgc‑‑gcgcgG‑‑CATCAACTTTGTGGTTGCGACCGGTCGGCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         <  2:6665115/89‑1 (MQ=255)
                                                                                  accgc‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         <  2:2479113/89‑1 (MQ=255)
                                                                                  accgc‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         >  2:4253665/5‑93 (MQ=255)
                                                                                  accgc‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         >  2:4337479/5‑93 (MQ=255)
                                                                                  accgc‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         <  2:6463337/89‑1 (MQ=255)
                                                                                   ccgc‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        <  1:3190090/90‑1 (MQ=255)
                                                                                      c‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTa     <  2:1842897/93‑1 (MQ=255)
                                                                                      c‑‑gcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTa     <  1:4108220/93‑1 (MQ=255)
                                                                                         gcgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCCCCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAc    <  1:945156/93‑1 (MQ=255)
                                                                                         gcgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAc    <  2:4742289/93‑1 (MQ=255)
                                                                                           gcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCt  >  2:4473920/1‑93 (MQ=255)
                                                                                               ||                                                                                         
GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000
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GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC                                                                                                 <  SRR4302543.6728197/94‑1 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.7813880/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.1099958/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.4384995/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.5427268/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.6349978/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑G                                                                                              >  SRR4302543.4561788/1‑94 (MQ=60)
  TTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCgg                                                                                               <  SRR4302543.9034/94‑3 (MQ=60)
  TTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCgg                                                                                               >  SRR4302543.4678281/1‑92 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCggc                                                                                              >  SRR4302543.4155883/1‑91 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCggc                                                                                              >  SRR4302543.738516/1‑91 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCggc                                                                                              >  SRR4302543.7462006/1‑91 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCggca                                                                                             >  SRR4302543.446173/1‑90 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCggca                                                                                             >  SRR4302543.7648589/1‑90 (MQ=60)
      TGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATC                                                                                           >  SRR4302543.1711687/1‑94 (MQ=60)
       GCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCA                                                                                          <  SRR4302543.2127060/94‑1 (MQ=60)
       GCGTCTGATGTAGATGGCACGTTACTTTCTCCTGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCA                                                                                          <  SRR4302543.7218479/94‑1 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAA                                                                                         >  SRR4302543.5481972/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAAC                                                                                        >  SRR4302543.4809814/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAAC                                                                                        <  SRR4302543.4253549/94‑1 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTT                                                                                      <  SRR4302543.4664894/94‑1 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTT                                                                                     >  SRR4302543.4338951/1‑94 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTT                                                                                     <  SRR4302543.5625741/94‑1 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTT                                                                                     <  SRR4302543.2574418/94‑1 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTT                                                                                     >  SRR4302543.1580612/1‑94 (MQ=60)
             GATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTG                                                                                    <  SRR4302543.81846/94‑1 (MQ=60)
             GATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTG                                                                                    <  SRR4302543.4521883/94‑1 (MQ=60)
              ATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGT                                                                                   >  SRR4302543.3374566/1‑94 (MQ=60)
              ATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGT                                                                                   >  SRR4302543.3241817/1‑94 (MQ=60)
              ATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGT                                                                                   >  SRR4302543.7732472/1‑94 (MQ=60)
                  AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTT                                                                               <  SRR4302543.4028853/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGC                                                                             >  SRR4302543.2734586/1‑94 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGC                                                                             <  SRR4302543.7708712/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGC                                                                             <  SRR4302543.7708711/94‑1 (MQ=60)
                      GGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAACCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGA                                                                           <  SRR4302543.5676839/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302543.4511966/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302543.2582563/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302543.4336430/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302543.4462932/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302543.6794496/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302543.6794497/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302543.516106/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCTCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       <  SRR4302543.4953141/94‑1 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302543.2464340/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCGTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       <  SRR4302543.4970355/94‑1 (MQ=60)
                           GTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      >  SRR4302543.6213586/1‑94 (MQ=60)
                           GTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGT                                                                      >  SRR4302543.837237/1‑94 (MQ=60)
                             TACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCG                                                                    >  SRR4302543.7584257/1‑94 (MQ=60)
                                TTTCTCCCGACCACACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCA                                                                 >  SRR4302543.7305327/1‑94 (MQ=60)
                                 TTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAATCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAC                                                                <  SRR4302543.4825316/94‑1 (MQ=60)
                                 TTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAC                                                                <  SRR4302543.4441325/94‑1 (MQ=60)
                                 TTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAC                                                                <  SRR4302543.4825315/94‑1 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               >  SRR4302543.1339444/1‑94 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               >  SRR4302543.1339445/1‑94 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               >  SRR4302543.7803577/1‑94 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               >  SRR4302543.3401190/1‑94 (MQ=60)
                                   CTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCA                                                              >  SRR4302543.2855139/1‑94 (MQ=60)
                                    TCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302543.3443093/94‑1 (MQ=60)
                                    CCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302543.7142273/94‑1 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302543.2034899/1‑94 (MQ=60)
                                      CCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGT                                                           >  SRR4302543.7235279/1‑94 (MQ=60)
                                      CCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGT                                                           >  SRR4302543.532578/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302543.6460252/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302543.5082139/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          <  SRR4302543.4073720/94‑1 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          <  SRR4302543.355893/94‑1 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302543.7657680/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302543.5982960/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302543.3155150/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302543.2879782/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         <  SRR4302543.1405711/94‑1 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302543.1107685/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         <  SRR4302543.288133/94‑1 (MQ=60)
                                        CACCATACTTTATCCCCTTACGCCAAAGAAACTCTGAAGCTCCTCACC‑‑GCGCGCGGCCTCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         <  SRR4302543.4725460/94‑1 (MQ=60)
                                         ACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAACACGTTGA                                                        >  SRR4302543.4296504/1‑94 (MQ=60)
                                         ACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCAACACGTTGA                                                        >  SRR4302543.4296505/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302543.4627266/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302543.3016376/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302543.5882713/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       <  SRR4302543.3354482/94‑1 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTCCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302543.1320196/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      <  SRR4302543.244946/94‑1 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      >  SRR4302543.7858363/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     <  SRR4302543.5720368/94‑1 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGG                                                     >  SRR4302543.723401/1‑94 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGTGCGCGG‑‑CATTAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302543.6447291/94‑1 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302543.6572963/94‑1 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302543.17758/94‑1 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302543.5090493/94‑1 (MQ=60)
                                             TACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTG                                                    <  SRR4302543.6447292/94‑1 (MQ=60)
                                              TCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302543.7406593/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302543.2043822/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302543.6543580/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302543.6849128/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302543.6849129/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   >  SRR4302543.3901445/1‑94 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302543.1869890/94‑1 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302543.6256540/94‑1 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  <  SRR4302543.331332/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302543.6151521/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302543.2438425/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302543.4955684/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302543.176261/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302543.3501247/1‑94 (MQ=60)
                                                CTTATCCCCCTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302543.1877421/94‑1 (MQ=60)
                                                 TTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTTGGGC                                                >  SRR4302543.3972129/1‑94 (MQ=60)
                                                 TTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGC                                                >  SRR4302543.672471/1‑94 (MQ=60)
                                                   TTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              <  SRR4302543.5893415/94‑1 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAAACTCTGAATCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              <  SRR4302543.7741639/94‑1 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            <  SRR4302543.3987213/94‑1 (MQ=60)
                                                     CCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            <  SRR4302543.5464582/94‑1 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302543.1838348/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302543.4097240/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302543.3074580/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302543.6738476/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302543.714273/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302543.1010519/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302543.2013420/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          >  SRR4302543.4715494/1‑94 (MQ=60)
                                                       CCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTC                                          <  SRR4302543.4189276/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302543.2194730/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302543.7599758/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         <  SRR4302543.5093696/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302543.7760438/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302543.3518270/1‑94 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAT                                     <  SRR4302543.7029040/94‑1 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAT                                     <  SRR4302543.7419015/94‑1 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    >  SRR4302543.7389590/1‑94 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   >  SRR4302543.586910/1‑94 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   <  SRR4302543.4314386/94‑1 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   >  SRR4302543.4359577/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  >  SRR4302543.100640/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  >  SRR4302543.456578/1‑94 (MQ=60)
                                                               CAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAT                                  <  SRR4302543.1961158/94‑1 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 <  SRR4302543.3714093/94‑1 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 >  SRR4302543.835124/1‑94 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 >  SRR4302543.7275857/1‑94 (MQ=60)
                                                                AAAGAAACTCTGAAGCTACTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 <  SRR4302543.7105486/94‑1 (MQ=60)
                                                                 AAGAAGCTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                <  SRR4302543.5415723/94‑1 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                <  SRR4302543.6482874/94‑1 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGGTGTGGGGCAAATTCGCGATCATCTGG                              >  SRR4302543.473780/1‑94 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302543.4658391/94‑1 (MQ=60)
                                                                   GAAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGG                              <  SRR4302543.4132799/94‑1 (MQ=60)
                                                                    AAACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGA                             <  SRR4302543.6573341/94‑1 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTTGGGCAAATTCGCGATAATCTGGAG                            >  SRR4302543.4422383/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            >  SRR4302543.822400/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302543.4011592/94‑1 (MQ=60)
                                                                      ACTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGA                           >  SRR4302543.3207599/1‑94 (MQ=60)
                                                                       CTCTGAAGCTGCTCCCC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.1629936/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGTTGTGGGGCAAATTCGCGATACTCTGGAGAT                          >  SRR4302543.4194550/1‑94 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          >  SRR4302543.5088686/1‑94 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.4607287/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.6336312/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          >  SRR4302543.6034503/1‑94 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.4234279/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.4607288/94‑1 (MQ=60)
                                                                       CTCTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAT                          <  SRR4302543.7775645/94‑1 (MQ=60)
                                                                         CTGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA                        <  SRR4302543.4891043/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       <  SRR4302543.7641011/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       >  SRR4302543.5660007/1‑94 (MQ=60)
                                                                          TGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       <  SRR4302543.699060/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       <  SRR4302543.5460584/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAACCGGGAGATTAA                       >  SRR4302543.5980552/1‑94 (MQ=60)
                                                                           GAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAG                      >  SRR4302543.3917533/1‑94 (MQ=60)
                                                                           GAAGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAG                      <  SRR4302543.7030463/94‑1 (MQ=60)
                                                                             AGCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302543.7428822/1‑94 (MQ=60)
                                                                              GCTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCT                   <  SRR4302543.3328104/94‑1 (MQ=60)
                                                                               CTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302543.691633/1‑94 (MQ=60)
                                                                               CTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302543.2366558/1‑94 (MQ=60)
                                                                               CTGCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  <  SRR4302543.74650/94‑1 (MQ=60)
                                                                                 GCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                <  SRR4302543.5899092/94‑1 (MQ=60)
                                                                                 GCTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                >  SRR4302543.7523210/1‑94 (MQ=60)
                                                                                  CTCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302543.6008312/1‑94 (MQ=60)
                                                                                   TCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302543.7685130/94‑1 (MQ=60)
                                                                                   TCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302543.765874/94‑1 (MQ=60)
                                                                                   TCACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              >  SRR4302543.4626436/1‑94 (MQ=60)
                                                                                    CACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             <  SRR4302543.5190795/94‑1 (MQ=60)
                                                                                     ACC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGA            <  SRR4302543.4667067/94‑1 (MQ=60)
                                                                                      GC‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         >  SRR4302543.534822/1‑94 (MQ=60)
                                                                                      GC‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302543.573790/94‑1 (MQ=60)
                                                                                      CC‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGAT           <  SRR4302543.3218858/94‑1 (MQ=60)
                                                                                       cgtGCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATT          <  SRR4302543.3247234/91‑1 (MQ=60)
                                                                                       C‑‑GCGCGG‑‑CATCATCTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302543.7050062/94‑1 (MQ=60)
                                                                                       C‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302543.7693911/94‑1 (MQ=60)
                                                                                       C‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302543.1630831/94‑1 (MQ=60)
                                                                                       C‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302543.4145184/94‑1 (MQ=60)
                                                                                       C‑‑GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTAC        <  SRR4302543.7359441/94‑1 (MQ=60)
                                                                                       C‑‑GCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATT          <  SRR4302543.2242791/94‑1 (MQ=60)
                                                                                          GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCCCCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACC       <  SRR4302543.953419/94‑1 (MQ=60)
                                                                                          GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACC       <  SRR4302543.2445409/94‑1 (MQ=60)
                                                                                          GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACC       <  SRR4302543.6358725/94‑1 (MQ=60)
                                                                                           CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      >  SRR4302543.6120881/1‑94 (MQ=60)
                                                                                            GCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTC     <  SRR4302543.3489497/94‑1 (MQ=60)
                                                                                            GCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTC     >  SRR4302543.614906/1‑94 (MQ=60)
                                                                                              GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   <  SRR4302543.580461/94‑1 (MQ=60)
                                                                                              GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   <  SRR4302543.769954/94‑1 (MQ=60)
                                                                                               G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  SRR4302543.3760047/1‑94 (MQ=60)
                                                                                               G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  <  SRR4302543.5589967/94‑1 (MQ=60)
                                                                                               G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  SRR4302543.3002986/1‑94 (MQ=60)
                                                                                               G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  <  SRR4302543.5674380/94‑1 (MQ=60)
                                                                                               |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACC‑‑GCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑