Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,822	G→A	L127L (CTG→CTA)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,822	0	G	A	99.6%	996.7 / NA	281	L127L (CTG→CTA)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (133/147); minor base C (1/0); total (134/147)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTA  >  NC_000913/4016732‑4016911

  aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGt                                                                                       <  1:1725315/93‑1 (MQ=255)

  aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGt                                                                                       >  1:2498729/1‑93 (MQ=255)

    tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTc                                                                                     <  1:1609569/93‑1 (MQ=255)

     ccATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTcc                                                                                    <  2:1903875/93‑1 (MQ=255)

      cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTccc                                                                                   >  1:1416164/1‑93 (MQ=255)

       aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTGCCCg                                                                                  <  2:2541969/93‑1 (MQ=255)

        tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCgg                                                                                 >  1:976403/1‑93 (MQ=255)

         gctcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGa                                                                                <  2:2362085/90‑1 (MQ=255)

            cAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATaa                                                                             <  1:1781314/93‑1 (MQ=255)

            cAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATaa                                                                             <  2:3869509/93‑1 (MQ=255)

             aaaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAac                                                                            <  2:3199504/93‑1 (MQ=255)

             aaaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAac                                                                            >  1:4308445/1‑93 (MQ=255)

              aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAacc                                                                           >  1:640282/1‑92 (MQ=255)

              aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAaca                                                                           >  1:1643402/1‑93 (MQ=255)

               aaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAacac                                                                          >  1:826768/1‑93 (MQ=255)

                 tGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCt                                                                        <  2:3304834/93‑1 (MQ=255)

                  gggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCTCGGATAACACCtt                                                                       >  1:2676748/1‑93 (MQ=255)

                  gggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCtt                                                                       <  2:4455804/93‑1 (MQ=255)

                     ccGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGa                                                                    <  2:4229211/93‑1 (MQ=255)

                     ccGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGa                                                                    <  1:2954346/93‑1 (MQ=255)

                       gcgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGAtt                                                                  >  1:1498057/1‑93 (MQ=255)

                        cgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTg                                                                 >  1:4273765/1‑93 (MQ=255)

                        cgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTg                                                                 <  1:3459543/93‑1 (MQ=255)

                        cgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGAGAACGCTGAGGCGCTACCGTTCCCGGATAACACATTTGATTg                                                                 >  2:1687331/1‑93 (MQ=255)

                         gagaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGc                                                                >  1:2811004/1‑93 (MQ=255)

                         gagaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGc                                                                <  2:2458531/93‑1 (MQ=255)

                         gagaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGc                                                                >  2:4343711/1‑93 (MQ=255)

                           gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCAt                                                              >  2:222283/1‑93 (MQ=255)

                           gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCAt                                                              <  2:680720/93‑1 (MQ=255)

                           gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCAt                                                              >  2:2627729/1‑93 (MQ=255)

                            aaGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATc                                                             <  2:1154821/93‑1 (MQ=255)

                            aaGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATc                                                             >  1:4683761/1‑93 (MQ=255)

                            aaGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATc                                                             <  1:1533740/93‑1 (MQ=255)

                              gCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCAc                                                           <  2:1081547/93‑1 (MQ=255)

                               cTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCAcc                                                          <  1:52270/93‑1 (MQ=255)

                                tGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCa                                                         <  2:4048963/93‑1 (MQ=255)

                                tGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCa                                                         >  1:1877428/1‑93 (MQ=255)

                                 gCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCAt                                                        <  1:3795396/93‑1 (MQ=255)

                                 gCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCAt                                                        <  1:2482524/93‑1 (MQ=255)

                                   gTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                                                      >  1:4515478/1‑93 (MQ=255)

                                   gTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                                                      >  1:4126035/1‑93 (MQ=255)

                                   gTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                                                      <  1:2921670/93‑1 (MQ=255)

                                   gTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATCGCATCACCAttt                                                      >  2:2887832/1‑93 (MQ=255)

                                    tattatCGGTGTGATTGACAGCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACTATTCCCGGCTAACGCCTTTGATTGCATCACCATTTc                                                     <  2:810624/90‑1 (MQ=255)

                                    tAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTc                                                     <  2:2836229/93‑1 (MQ=255)

                                      atatCGGTGTGATTGTCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGt                                                   <  1:1971352/93‑1 (MQ=255)

                                      atatCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGt                                                   >  1:1397442/1‑93 (MQ=255)

                                        atCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGttt                                                 <  1:3631907/93‑1 (MQ=255)

                                         tCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTg                                                <  2:3731868/93‑1 (MQ=255)

                                         tCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTg                                                <  1:1296559/93‑1 (MQ=255)

                                            gtgtGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTc                                             >  2:19300/1‑93 (MQ=255)

                                               tgATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGc                                          >  2:1418930/1‑93 (MQ=255)

                                               tgATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGc                                          >  2:574266/1‑93 (MQ=255)

                                               tgATTGGAAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCTCTACCGTTCCCGGATAACGCCTTTGATTGCATCACCATTTCGTTTGGTCTGc                                          <  2:209896/93‑1 (MQ=255)

                                                 aTTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGt                                        <  2:890180/93‑1 (MQ=255)

                                                 aTTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGt                                        >  1:1056445/1‑93 (MQ=255)

                                                 aTTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGt                                        >  1:4623351/1‑93 (MQ=255)

                                                  ttGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTa                                       <  2:2155747/93‑1 (MQ=255)

                                                   tGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTaa                                      <  1:1332827/93‑1 (MQ=255)

                                                    ggCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAc                                     >  1:3496487/1‑93 (MQ=255)

                                                     gCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACg                                    >  1:3956560/1‑93 (MQ=255)

                                                       aaCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTc                                  <  2:1119166/93‑1 (MQ=255)

                                                        aCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCa                                 >  1:3065826/1‑93 (MQ=255)

                                                        aCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCa                                 >  2:2286251/1‑93 (MQ=255)

                                                        aCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCa                                 >  2:252786/1‑93 (MQ=255)

                                                          gTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCAcc                               >  2:4373019/1‑93 (MQ=255)

                                                           ttGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCg                              >  1:750971/1‑93 (MQ=255)

                                                           ttGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCg                              <  2:1816038/93‑1 (MQ=255)

                                                            tGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGa                             >  2:1465614/1‑93 (MQ=255)

                                                             gAGTGTGTTCAGGCGAACGCTGAGTCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAc                            >  2:1612530/1‑93 (MQ=255)

                                                              aGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACa                           >  2:3025694/1‑93 (MQ=255)

                                                                tgtGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACaaa                         >  1:2114109/3‑93 (MQ=255)

                                                                gttGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACaaa                         <  2:1234940/91‑1 (MQ=255)

                                                                 ttGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAg                        <  2:259903/92‑1 (MQ=255)

                                                                  tGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGa                       <  1:2343797/93‑1 (MQ=255)

                                                                  tGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGa                       <  2:2045280/93‑1 (MQ=255)

                                                                  tGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGa                       >  1:2205899/1‑93 (MQ=255)

                                                                   gTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAt                      >  1:4409698/1‑93 (MQ=255)

                                                                   gTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAt                      <  2:4414354/93‑1 (MQ=255)

                                                                    ttCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATACCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAtata                     >  2:4663161/1‑93 (MQ=255)

                                                                     tCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATaa                    <  2:3457877/93‑1 (MQ=255)

                                                                       aGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAg                  <  2:565619/93‑1 (MQ=255)

                                                                        ggCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGc                 >  2:2508992/1‑93 (MQ=255)

                                                                           gAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACt              >  2:2504708/1‑93 (MQ=255)

                                                                           gAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACt              >  2:614629/1‑93 (MQ=255)

                                                                           gAACGCTGACGCTCTACCGCCCCCGAATAACCCCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACt              <  2:4086957/93‑1 (MQ=255)

                                                                            aaCGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTg             >  2:2726918/1‑93 (MQ=255)

                                                                             aCGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGc            >  1:1171750/1‑93 (MQ=255)

                                                                             aCGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGc            <  1:3257535/93‑1 (MQ=255)

                                                                             aCGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGc            <  2:1999615/93‑1 (MQ=255)

                                                                              cGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCg           <  1:3191659/93‑1 (MQ=255)

                                                                              cGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCg           <  2:3755962/93‑1 (MQ=255)

                                                                                  gAGGCGCTCCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAC‑GCGCTCaa      >  1:707521/1‑93 (MQ=255)

                                                                                  gAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCa       <  1:2380859/93‑1 (MQ=255)

                                                                                    ggCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAAt     <  2:4563788/93‑1 (MQ=255)

                                                                                    ggCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAAt     <  1:4027566/93‑1 (MQ=255)

                                                                                     gcgcTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATg    <  2:3600435/93‑1 (MQ=255)

                                                                                     gcgcTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATg    >  2:62641/1‑93 (MQ=255)

                                                                                      cgcTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGt   >  2:4643615/1‑93 (MQ=255)

                                                                                       gcTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTCGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTa  <  2:2857935/93‑1 (MQ=255)

TGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTA  >  NC_000913/4016732‑4016911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGC  >  NC_000913/4016729‑4016915

CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTA                                                                                               >  SRR4302535.1982894/1‑94 (MQ=60)

CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTA                                                                                               >  SRR4302535.1812591/1‑94 (MQ=60)

 AATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTAC                                                                                              >  SRR4302535.3882477/1‑94 (MQ=60)

  ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACC                                                                                             <  SRR4302535.3537503/94‑1 (MQ=60)

  ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACC                                                                                             <  SRR4302535.3091484/94‑1 (MQ=60)

  ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACC                                                                                             <  SRR4302535.4220922/94‑1 (MQ=60)

   TGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCG                                                                                            >  SRR4302535.3262358/1‑94 (MQ=60)

    GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGT                                                                                           >  SRR4302535.2516756/1‑94 (MQ=60)

     AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTT                                                                                          <  SRR4302535.1737793/94‑1 (MQ=60)

      ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTC                                                                                         <  SRR4302535.4568437/94‑1 (MQ=60)

      ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTC                                                                                         <  SRR4302535.622323/94‑1 (MQ=60)

       TCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCC                                                                                        <  SRR4302535.1621174/94‑1 (MQ=60)

        CTATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCC                                                                                       <  SRR4302535.3063998/94‑1 (MQ=60)

        CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCC                                                                                       >  SRR4302535.1241909/1‑94 (MQ=60)

        CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCC                                                                                       >  SRR4302535.1426273/1‑94 (MQ=60)

         CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCG                                                                                      <  SRR4302535.3373467/94‑1 (MQ=60)

          ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGG                                                                                     <  SRR4302535.1617630/94‑1 (MQ=60)

          ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGG                                                                                     >  SRR4302535.974589/1‑94 (MQ=60)

          ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGG                                                                                     >  SRR4302535.983332/1‑94 (MQ=60)

           TGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGA                                                                                    >  SRR4302535.2506496/1‑94 (MQ=60)

            GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGAT                                                                                   <  SRR4302535.3013147/94‑1 (MQ=60)

             CTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATA                                                                                  <  SRR4302535.2763629/94‑1 (MQ=60)

              TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAA                                                                                 <  SRR4302535.1775428/94‑1 (MQ=60)

               CAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAAC                                                                                >  SRR4302535.4337921/1‑94 (MQ=60)

               CAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAAC                                                                                <  SRR4302535.1794205/94‑1 (MQ=60)

                AAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACC                                                                               >  SRR4302535.644756/1‑94 (MQ=60)

                AAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACA                                                                               >  SRR4302535.1655280/1‑94 (MQ=60)

                 AAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACAC                                                                              >  SRR4302535.832557/1‑94 (MQ=60)

                   ATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCT                                                                            >  SRR4302535.3206148/1‑94 (MQ=60)

                    TGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCTCGGATAACACCTT                                                                           >  SRR4302535.2695897/1‑94 (MQ=60)

                    TGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTT                                                                           <  SRR4302535.1640577/94‑1 (MQ=60)

                    TGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTT                                                                           >  SRR4302535.2249278/1‑94 (MQ=60)

                     GGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTT                                                                          <  SRR4302535.1524647/94‑1 (MQ=60)

                     GGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTT                                                                          <  SRR4302535.3529140/94‑1 (MQ=60)

                        CCGCGAGAAGCTGCGTAGTATCGGTGTGATTGGCAATGTTGAGTGTGTTCAGGCGAACGCTGAGGAGCTACCGTTCCCGGATAACACCTTTGAT                                                                       >  SRR4302535.3834297/1‑94 (MQ=60)

                        CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGAT                                                                       <  SRR4302535.2975098/94‑1 (MQ=60)

                        CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGAT                                                                       >  SRR4302535.4468853/1‑94 (MQ=60)

                        CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGAT                                                                       >  SRR4302535.3509213/1‑94 (MQ=60)

                        CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGAT                                                                       <  SRR4302535.725377/94‑1 (MQ=60)

                        CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACAGTTCCCGGATAACACCTTTGAT                                                                       <  SRR4302535.4680154/94‑1 (MQ=60)

                         CGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATT                                                                      >  SRR4302535.1508829/1‑94 (MQ=60)

                         CGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATT                                                                      >  SRR4302535.1766743/1‑94 (MQ=60)

                          cgGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTG                                                                     <  SRR4302535.2876211/92‑1 (MQ=60)

                          GCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTG                                                                     >  SRR4302535.4303020/1‑94 (MQ=60)

                           CGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGC                                                                    >  SRR4302535.2830988/1‑94 (MQ=60)

                           CGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGC                                                                    <  SRR4302535.3483597/94‑1 (MQ=60)

                           CGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGC                                                                    >  SRR4302535.1410922/1‑94 (MQ=60)

                             AGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCAT                                                                  <  SRR4302535.4004062/94‑1 (MQ=60)

                              GAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATC                                                                 >  SRR4302535.4715436/1‑94 (MQ=60)

                              GAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATC                                                                 >  SRR4302535.1962857/1‑94 (MQ=60)

                               AAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCA                                                                <  SRR4302535.1544790/94‑1 (MQ=60)

                                AGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCAC                                                               <  SRR4302535.4075365/94‑1 (MQ=60)

                                 GCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACC                                                              >  SRR4302535.434635/1‑94 (MQ=60)

                                 GCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACC                                                              >  SRR4302535.2602251/1‑94 (MQ=60)

                                  CTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCA                                                             <  SRR4302535.52651/94‑1 (MQ=60)

                                  CTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCA                                                             >  SRR4302535.1890953/1‑94 (MQ=60)

                                  CTGCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCA                                                             >  SRR4302535.378605/1‑94 (MQ=60)

                                    GCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATT                                                           >  SRR4302535.442044/1‑94 (MQ=60)

                                    GCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATT                                                           <  SRR4302535.3821559/94‑1 (MQ=60)

                                    GCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATT                                                           <  SRR4302535.2503274/94‑1 (MQ=60)

                                    GCGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATT                                                           <  SRR4302535.2500424/94‑1 (MQ=60)

                                     CGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                                                          >  SRR4302535.4546437/1‑94 (MQ=60)

                                     CGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                                                          >  SRR4302535.4154271/1‑94 (MQ=60)

                                     CGTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                                                          >  SRR4302535.2196964/1‑94 (MQ=60)

                                      GTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCGTCACCATTTC                                                         <  SRR4302535.1473504/94‑1 (MQ=60)

                                      GTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTC                                                         <  SRR4302535.2942280/94‑1 (MQ=60)

                                      GTAATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTC                                                         <  SRR4302535.4145123/94‑1 (MQ=60)

                                        AATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGT                                                       >  SRR4302535.1407417/1‑94 (MQ=60)

                                         ATATCGGTGTGATTGTCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTT                                                      <  SRR4302535.1985549/94‑1 (MQ=60)

                                         ATATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTT                                                      >  SRR4302535.1424926/1‑94 (MQ=60)

                                          TATCGGTGTGATTGGCAGCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTT                                                     <  SRR4302535.2011297/94‑1 (MQ=60)

                                           ATGGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTG                                                    <  SRR4302535.1389567/94‑1 (MQ=60)

                                           ATCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTG                                                    <  SRR4302535.3657081/94‑1 (MQ=60)

                                            TCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGG                                                   <  SRR4302535.1305809/94‑1 (MQ=60)

                                            TCGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGG                                                   <  SRR4302535.932680/94‑1 (MQ=60)

                                             CGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGT                                                  >  SRR4302535.696858/1‑94 (MQ=60)

                                             CGGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGT                                                  <  SRR4302535.2867547/94‑1 (MQ=60)

                                              GGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATGACACCTTTGATTGCATCACCATTTCGTTTGGTC                                                 >  SRR4302535.1342282/1‑94 (MQ=60)

                                              GGTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTC                                                 <  SRR4302535.3506793/94‑1 (MQ=60)

                                               GTGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCT                                                >  SRR4302535.2959676/1‑94 (MQ=60)

                                                TGTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTG                                               <  SRR4302535.4034253/94‑1 (MQ=60)

                                                 GTGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGC                                              <  SRR4302535.1079958/94‑1 (MQ=60)

                                                  TGATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCG                                             <  SRR4302535.1889987/94‑1 (MQ=60)

                                                   GATTGGCATCGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGT                                            <  SRR4302535.362311/94‑1 (MQ=60)

                                                   GATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGT                                            >  SRR4302535.1063956/1‑94 (MQ=60)

                                                   GATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGT                                            >  SRR4302535.4654752/1‑94 (MQ=60)

                                                    ATTGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTA                                           >  SRR4302535.4260562/1‑94 (MQ=60)

                                                      TGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAC                                         >  SRR4302535.3520786/1‑94 (MQ=60)

                                                      TGGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAC                                         <  SRR4302535.1342333/94‑1 (MQ=60)

                                                       GGCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACG                                        >  SRR4302535.3983574/1‑94 (MQ=60)

                                                        GCAACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGT                                       <  SRR4302535.1697785/94‑1 (MQ=60)

                                                         CAACGGTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTC                                      <  SRR4302535.75445/94‑1 (MQ=60)

                                                          AACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA                                     >  SRR4302535.3087253/1‑94 (MQ=60)

                                                          AACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA                                     <  SRR4302535.474817/94‑1 (MQ=60)

                                                          AACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA                                     >  SRR4302535.630433/1‑94 (MQ=60)

                                                          AACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA                                     <  SRR4302535.2412095/94‑1 (MQ=60)

                                                           ACGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCAC                                    >  SRR4302535.1553443/1‑94 (MQ=60)

                                                            CGTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAAAACCTTTGATTGCATCACCCTTTCGTTTGGTCTGCCTAACGTCACC                                   >  SRR4302535.3730816/1‑94 (MQ=60)

                                                             GTTGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCG                                  >  SRR4302535.756263/1‑94 (MQ=60)

                                                               TGAGTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAC                                <  SRR4302535.432503/94‑1 (MQ=60)

                                                                  GTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAA                             >  SRR4302535.2129367/1‑94 (MQ=60)

                                                                  GTGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAA                             <  SRR4302535.4454273/94‑1 (MQ=60)

                                                                   TGTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAG                            >  SRR4302535.1223502/1‑94 (MQ=60)

                                                                    GTGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGA                           >  SRR4302535.2221609/1‑94 (MQ=60)

                                                                     TGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAT                          <  SRR4302535.2360568/94‑1 (MQ=60)

                                                                     TGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAT                          <  SRR4302535.463555/94‑1 (MQ=60)

                                                                     TGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAT                          >  SRR4302535.4439874/1‑94 (MQ=60)

                                                                     TGTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTCCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAT                          <  SRR4302535.3325585/94‑1 (MQ=60)

                                                                      GTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATA                         >  SRR4302535.1524348/1‑94 (MQ=60)

                                                                      GTTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATA                         <  SRR4302535.4325868/94‑1 (MQ=60)

                                                                       TTCAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAA                        <  SRR4302535.314686/94‑1 (MQ=60)

                                                                         CAGGCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAG                      <  SRR4302535.2599431/94‑1 (MQ=60)

                                                                            GCGAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAC                   >  SRR4302535.222882/1‑94 (MQ=60)

                                                                              GAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTG                 >  SRR4302535.2128051/1‑94 (MQ=60)

                                                                              GAACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTG                 <  SRR4302535.133601/94‑1 (MQ=60)

                                                                               AACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGC                <  SRR4302535.4697794/94‑1 (MQ=60)

                                                                               AACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGC                >  SRR4302535.1180117/1‑94 (MQ=60)

                                                                               AACGCTGAGGCGCTACCGTTACCGGATAACACCTTTGATTGCATCACCATTTCGCTTGGTCTGCGTAACGTCACCGACAAAGATAACGCACTGC                >  SRR4302535.1432497/1‑94 (MQ=60)

                                                                                ACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCG               <  SRR4302535.3280169/94‑1 (MQ=60)

                                                                                ACGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCG               >  SRR4302535.1853819/1‑94 (MQ=60)

                                                                                 CGCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGT              <  SRR4302535.3213736/94‑1 (MQ=60)

                                                                                  GCTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTT             <  SRR4302535.1701849/94‑1 (MQ=60)

                                                                                   CTGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTC            >  SRR4302535.2380136/1‑94 (MQ=60)

                                                                                    TGAGGCGCTCCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAC‑GCGCTCAA          >  SRR4302535.712486/1‑94 (MQ=60)

                                                                                    TGAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCA           >  SRR4302535.765521/1‑94 (MQ=60)

                                                                                     GAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAA          <  SRR4302535.2397957/94‑1 (MQ=60)

                                                                                     GAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAA          <  SRR4302535.2210414/94‑1 (MQ=60)

                                                                                     GAGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAA          >  SRR4302535.138740/1‑94 (MQ=60)

                                                                                      AGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAAT         <  SRR4302535.2828255/94‑1 (MQ=60)

                                                                                      AGGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAAT         <  SRR4302535.3889328/94‑1 (MQ=60)

                                                                                       GGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATG        <  SRR4302535.4055124/94‑1 (MQ=60)

                                                                                       GGCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATG        <  SRR4302535.769944/94‑1 (MQ=60)

                                                                                        GCGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGT       <  SRR4302535.1918739/94‑1 (MQ=60)

                                                                                         CGCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTA      >  SRR4302535.2956120/1‑94 (MQ=60)

                                                                                          GCTACCGTTCCCGGATAACTCCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTAT     <  SRR4302535.47421/94‑1 (MQ=60)

                                                                                          GCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTAT     >  SRR4302535.1099620/1‑94 (MQ=60)

                                                                                          GCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTAT     >  SRR4302535.3942701/1‑94 (MQ=60)

                                                                                          GCTACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTAT     <  SRR4302535.2449141/94‑1 (MQ=60)

                                                                                            TACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCG   <  SRR4302535.3982726/94‑1 (MQ=60)

                                                                                            TACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCG   <  SRR4302535.3170105/94‑1 (MQ=60)

                                                                                             TCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGC  <  SRR4302535.2262513/94‑1 (MQ=60)

                                                                                             ACCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGC  >  SRR4302535.4240558/1‑94 (MQ=60)

CAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGC  >  NC_000913/4016729‑4016915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`