Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,021,684 Δ1 bp coding (542/609 nt)|coding (1/243 nt) b4556 →
tatD →		 hypothetical protein
Mg‑dependent DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,021,6830C.100.0% 866.5 / NA 190coding (541/609 nt)b4556hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (97/93);  total (97/93)

Only 100 of 205 total aligned reads displayed.

TCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT  >  NC_000913/4021592‑4021770
                                                                                            |                                                                                       
tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAAcg                                                                                        >  2:172103/1‑92 (MQ=255)
 cttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAAcga                                                                                       <  2:4460882/93‑3 (MQ=255)
 cttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAAcga                                                                                       <  1:470758/93‑3 (MQ=255)
   tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAAcgacg                                                                                     >  2:1517654/1‑89 (MQ=255)
    cATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGAcgc                                                                                   <  1:2292627/93‑1 (MQ=255)
     aTTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGTA‑CGAcgcg                                                                                  >  2:2342379/1‑93 (MQ=255)
        gCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGACGAA‑CGACGCGGAc                                                                               >  1:1956815/1‑93 (MQ=255)
         cTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACt                                                                              >  2:2812012/1‑93 (MQ=255)
          tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                             >  1:1277919/1‑93 (MQ=255)
          tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                             >  1:1448913/1‑93 (MQ=255)
          tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                             <  2:33163/93‑1 (MQ=255)
             aCCGGCACACGCGAAGAGATGCAGGCGAGCGTGGCGCATGGAATTTATATAGGCATT‑ACCGGTTAGGTTTGCGATGCA‑CGACGCGGACTggtg                                                                          >  2:463558/1‑91 (MQ=255)
              ccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGc                                                                         <  1:1947128/93‑1 (MQ=255)
              ccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGc                                                                         <  1:7462/93‑1 (MQ=255)
              ccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGc                                                                         <  2:1068139/93‑1 (MQ=255)
              ccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGc                                                                         <  2:2583704/93‑1 (MQ=255)
              ccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGc                                                                         >  2:3444043/1‑93 (MQ=255)
                ggCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTg                                                                       >  1:1715933/1‑93 (MQ=255)
                ggCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTg                                                                       >  1:2630531/1‑93 (MQ=255)
                 gCAGACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                      <  1:964301/93‑1 (MQ=255)
                 gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                      <  2:2666323/93‑1 (MQ=255)
                 gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                      <  1:1818370/93‑1 (MQ=255)
                 gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                      >  2:359683/1‑93 (MQ=255)
                 gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                      >  1:2558695/1‑93 (MQ=255)
                  cacaCGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCg                                                                     >  1:1224803/1‑93 (MQ=255)
                   acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                    >  2:4393169/1‑93 (MQ=255)
                   acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                    >  1:4191563/1‑93 (MQ=255)
                    cacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCggg                                                                   >  2:3138147/1‑93 (MQ=255)
                        cgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACt                                                               <  2:917588/93‑1 (MQ=255)
                         gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                              <  1:2030221/93‑1 (MQ=255)
                          aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                             <  1:2730055/93‑1 (MQ=255)
                           agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                            <  2:1295947/93‑1 (MQ=255)
                            gagaTGCAGGCGTGCGTGGCGCATGGAATTTATGTCGGCATT‑ACGGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTg                                                           <  2:1935775/93‑1 (MQ=255)
                            gagaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTAACCGGTTGGG‑TTGGGGTGGA‑CGACAGGGACTGGGGGCTCGGGAACttttc                                                           >  2:3073939/1‑92 (MQ=255)
                              gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGGACTTTTGcc                                                         >  2:539402/1‑93 (MQ=255)
                              gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGcc                                                         <  1:161252/93‑1 (MQ=255)
                                tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                       <  2:609231/93‑1 (MQ=255)
                                  cAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTg                                                     >  1:2589909/1‑93 (MQ=255)
                                   aGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGa                                                    >  1:4486560/1‑93 (MQ=255)
                                    ggCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                   <  1:4600785/93‑1 (MQ=255)
                                      cgtgcgtgGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                 <  2:2353712/93‑1 (MQ=255)
                                       gtgcgtgGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                <  1:2246157/93‑1 (MQ=255)
                                       gtgcgtgGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                >  2:3055302/1‑93 (MQ=255)
                                         gcgtgGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                              >  2:3382601/1‑93 (MQ=255)
                                            tgGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggcgg                                           >  2:1769750/1‑93 (MQ=255)
                                             ggCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGTCGTTGATTCCGGCGGa                                          >  1:2176352/1‑93 (MQ=255)
                                             ggCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGa                                          >  1:2286069/1‑93 (MQ=255)
                                                gcATGGAATTTATATCGGCATT‑ACGGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                       <  2:3516245/93‑1 (MQ=255)
                                                gcATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                       >  1:100884/1‑93 (MQ=255)
                                                gcATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                       >  1:1999522/1‑93 (MQ=255)
                                                gcATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                       >  1:3138506/1‑93 (MQ=255)
                                                 cATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaaa                                      >  1:1303757/1‑93 (MQ=255)
                                                  aTGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                     >  2:3488014/1‑93 (MQ=255)
                                                  aTGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                     >  1:1732321/1‑93 (MQ=255)
                                                  aTGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                     >  1:3865234/1‑93 (MQ=255)
                                                  aTGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                     >  1:2375077/1‑93 (MQ=255)
                                                  aTGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                     <  2:2053396/93‑1 (MQ=255)
                                                   tGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                    >  2:1396403/1‑93 (MQ=255)
                                                    ggAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGTAAAATTa                                   >  2:4417417/1‑93 (MQ=255)
                                                    ggAATTTAGATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTa                                   <  2:2274706/93‑1 (MQ=255)
                                                     gAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                  <  1:834736/93‑1 (MQ=255)
                                                     gAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                  >  1:1067769/1‑93 (MQ=255)
                                                     gAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                  >  2:263415/1‑93 (MQ=255)
                                                       aTTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTg                                >  2:3208578/1‑93 (MQ=255)
                                                       aTTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTg                                >  1:4322995/1‑93 (MQ=255)
                                                       aTTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTg                                <  1:3453739/93‑1 (MQ=255)
                                                        tttATATCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                               >  2:122746/1‑93 (MQ=255)
                                                          tataTCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                             <  2:1739366/93‑1 (MQ=255)
                                                          tataTCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                             <  1:4256245/93‑1 (MQ=255)
                                                            tatCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                           >  1:3161007/1‑93 (MQ=255)
                                                            tatCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                           <  1:1531124/93‑1 (MQ=255)
                                                              tCGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGaaa                         <  2:767717/93‑1 (MQ=255)
                                                               cGGCATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAc                        <  1:3395077/93‑1 (MQ=255)
                                                                  cATT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGa                     <  1:3149247/93‑1 (MQ=255)
                                                                   aTT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                    <  2:1476670/93‑1 (MQ=255)
                                                                   aTT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                    >  1:3787526/1‑93 (MQ=255)
                                                                   aTT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                    <  2:1111308/93‑1 (MQ=255)
                                                                   aTT‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                    >  2:1898921/1‑93 (MQ=255)
                                                                     t‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgg                  >  1:3409525/1‑92 (MQ=255)
                                                                     t‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                  <  2:3759878/93‑1 (MQ=255)
                                                                     t‑ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                  <  1:1123824/93‑1 (MQ=255)
                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc               <  2:2105174/93‑1 (MQ=255)
                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg              >  1:3948232/1‑93 (MQ=255)
                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg              >  1:1745720/1‑93 (MQ=255)
                                                                           gTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGt             >  2:819180/1‑93 (MQ=255)
                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa            <  1:561938/93‑1 (MQ=255)
                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa            <  1:2310526/93‑1 (MQ=255)
                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc          <  1:3709984/93‑1 (MQ=255)
                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc          <  1:2137141/93‑1 (MQ=255)
                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc          <  2:3959174/93‑1 (MQ=255)
                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc          >  1:606541/1‑93 (MQ=255)
                                                                               ggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCt         >  1:2604195/1‑93 (MQ=255)
                                                                                gTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTg        <  1:3078794/93‑1 (MQ=255)
                                                                                 tttGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGc       <  1:3006764/93‑1 (MQ=255)
                                                                                 tttGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGc       <  1:1801897/93‑1 (MQ=255)
                                                                                  ttGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCt      <  2:2126547/93‑1 (MQ=255)
                                                                                   tGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTc     <  2:4550623/93‑1 (MQ=255)
                                                                                    gCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTcc    <  2:4195980/93‑1 (MQ=255)
                                                                                     cGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTccc   >  2:2152800/1‑93 (MQ=255)
                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt  >  2:4366058/1‑93 (MQ=255)
                                                                                            |                                                                                       
TCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATT‑ACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT  >  NC_000913/4021592‑4021770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA  >  NC_000913/4021591‑4021775
                                                                                             |                                                                                           
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                             >  SRR4302535.1612840/1‑94 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                          <  SRR4302535.1991351/94‑1 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                          <  SRR4302535.474051/94‑1 (MQ=60)
   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                         <  SRR4302535.3355472/94‑1 (MQ=60)
     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                       <  SRR4302535.1756854/94‑1 (MQ=60)
     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                       <  SRR4302535.2309026/94‑1 (MQ=60)
      ATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGG                                                                                      >  SRR4302535.2752855/1‑94 (MQ=60)
      ATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGG                                                                                      <  SRR4302535.1122312/94‑1 (MQ=60)
       ATGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGA                                                                                     <  SRR4302535.4699617/94‑1 (MQ=60)
        TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAC                                                                                    >  SRR4302535.683540/1‑94 (MQ=60)
        TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAC                                                                                    <  SRR4302535.257752/94‑1 (MQ=60)
        TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAC                                                                                    >  SRR4302535.1818525/1‑94 (MQ=60)
        TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGACGAA‑CGACGCGGAC                                                                                    >  SRR4302535.1970898/1‑94 (MQ=60)
          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                  >  SRR4302535.1729177/1‑94 (MQ=60)
          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                  >  SRR4302535.1287048/1‑94 (MQ=60)
          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                  >  SRR4302535.1459274/1‑94 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                             <  SRR4302535.570720/94‑1 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                             <  SRR4302535.7521/94‑1 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                             >  SRR4302535.2303854/1‑94 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                             <  SRR4302535.1961146/94‑1 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                            >  SRR4302535.1728346/1‑94 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                            >  SRR4302535.2649375/1‑94 (MQ=60)
                 GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGC                                                                           >  SRR4302535.2577113/1‑94 (MQ=60)
                  GCAGACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                          <  SRR4302535.971154/94‑1 (MQ=60)
                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                          <  SRR4302535.1831511/94‑1 (MQ=60)
                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                          >  SRR4302535.1233562/1‑94 (MQ=60)
                   CACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGG                                                                         >  SRR4302535.4220248/1‑94 (MQ=60)
                    ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                        >  SRR4302535.2290701/1‑94 (MQ=60)
                       CGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAC                                                                     <  SRR4302535.3874586/94‑1 (MQ=60)
                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACT                                                                    >  SRR4302535.1526771/1‑94 (MQ=60)
                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                   <  SRR4302535.824927/94‑1 (MQ=60)
                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                  <  SRR4302535.2044853/94‑1 (MQ=60)
                           AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                 <  SRR4302535.2749545/94‑1 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                >  SRR4302535.3166230/1‑94 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                >  SRR4302535.1032918/1‑94 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                >  SRR4302535.2211023/1‑94 (MQ=60)
                             GAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGC                                                               <  SRR4302535.2612305/94‑1 (MQ=60)
                               GATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCG                                                             <  SRR4302535.162383/94‑1 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                            >  SRR4302535.4571126/1‑94 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                            <  SRR4302535.4252261/94‑1 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                            >  SRR4302535.3419164/1‑94 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAATTTTGCCGT                                                            >  SRR4302535.196055/1‑94 (MQ=60)
                                  GCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTG                                                          >  SRR4302535.2608507/1‑94 (MQ=60)
                                   CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                         >  SRR4302535.4517360/1‑94 (MQ=60)
                                   CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGGTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                         >  SRR4302535.2022583/1‑94 (MQ=60)
                                    AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                        >  SRR4302535.1675499/1‑94 (MQ=60)
                                     GGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATT                                                       <  SRR4302535.4632075/94‑1 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                    <  SRR4302535.2262128/94‑1 (MQ=60)
                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTTCGGCG                                                 >  SRR4302535.2133641/1‑94 (MQ=60)
                                             TGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGTCGTTGATTCCGGCGGA                                               >  SRR4302535.2191908/1‑94 (MQ=60)
                                             TGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGA                                               >  SRR4302535.3474580/1‑94 (MQ=60)
                                             TGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGA                                               >  SRR4302535.2302402/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                            >  SRR4302535.3778613/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                            >  SRR4302535.3160347/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                            >  SRR4302535.101595/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                            >  SRR4302535.2013926/1‑94 (MQ=60)
                                                 GCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAA                                           >  SRR4302535.1313066/1‑94 (MQ=60)
                                                  CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                          >  SRR4302535.3891820/1‑94 (MQ=60)
                                                  CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                          >  SRR4302535.1744842/1‑94 (MQ=60)
                                                  CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                          >  SRR4302535.2392142/1‑94 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                         >  SRR4302535.3176269/1‑94 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                         >  SRR4302535.521378/1‑94 (MQ=60)
                                                    TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTA                                        <  SRR4302535.1291183/94‑1 (MQ=60)
                                                    TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTA                                        <  SRR4302535.915313/94‑1 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                       >  SRR4302535.1654747/1‑94 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                       >  SRR4302535.1075364/1‑94 (MQ=60)
                                                     AGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                       <  SRR4302535.1922340/94‑1 (MQ=60)
                                                      GAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACT                                      <  SRR4302535.840589/94‑1 (MQ=60)
                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                     >  SRR4302535.4352569/1‑94 (MQ=60)
                                                        ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                    <  SRR4302535.3477747/94‑1 (MQ=60)
                                                        ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                    <  SRR4302535.1804608/94‑1 (MQ=60)
                                                         TTTATATCGGCATTACCGGTTGGGTTTGCGATGGA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAT                                   <  SRR4302535.1150289/94‑1 (MQ=60)
                                                          TTATATCGGCATTACCGGCTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAACATTACTGATC                                  >  SRR4302535.2320042/1‑94 (MQ=60)
                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                 <  SRR4302535.4285402/94‑1 (MQ=60)
                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                 <  SRR4302535.4493867/94‑1 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                >  SRR4302535.3182935/1‑94 (MQ=60)
                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                               <  SRR4302535.1542154/94‑1 (MQ=60)
                                                              ATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAA                              <  SRR4302535.3881853/94‑1 (MQ=60)
                                                               TCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAGGCGGACAGGAGCTGCGGGAAATTTTGCCGTTGATTGCGGCGGAAAAATTACTCATCGAAAC                             >  SRR4302535.3537170/1‑94 (MQ=60)
                                                                CGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACT                            <  SRR4302535.3418699/94‑1 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                         >  SRR4302535.3924675/1‑94 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                         >  SRR4302535.3813652/1‑94 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                         <  SRR4302535.3171139/94‑1 (MQ=60)
                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                        >  SRR4302535.244856/1‑94 (MQ=60)
                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGG                       >  SRR4302535.3433251/1‑94 (MQ=60)
                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                       >  SRR4302535.306394/1‑94 (MQ=60)
                                                                      TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                      <  SRR4302535.1131844/94‑1 (MQ=60)
                                                                      TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                      >  SRR4302535.313718/1‑94 (MQ=60)
                                                                        CCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCAGCGGAAAAATTACTGATCGAAACTGATGCGCC                    >  SRR4302535.225044/1‑94 (MQ=60)
                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                   >  SRR4302535.1758352/1‑94 (MQ=60)
                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                   >  SRR4302535.3975187/1‑94 (MQ=60)
                                                                          GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGT                  >  SRR4302535.2167651/1‑94 (MQ=60)
                                                                          GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGT                  >  SRR4302535.26373/1‑94 (MQ=60)
                                                                           GTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGACCGAAACTGATGCGCCGTA                 >  SRR4302535.4346390/1‑94 (MQ=60)
                                                                            TTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                <  SRR4302535.4090797/94‑1 (MQ=60)
                                                                            TTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                <  SRR4302535.565867/94‑1 (MQ=60)
                                                                            TTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                <  SRR4302535.2327063/94‑1 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC               >  SRR4302535.610777/1‑94 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC               <  SRR4302535.1248877/94‑1 (MQ=60)
                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT              <  SRR4302535.3735678/94‑1 (MQ=60)
                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT              >  SRR4302535.2622890/1‑94 (MQ=60)
                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT              <  SRR4302535.2152534/94‑1 (MQ=60)
                                                                               GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTG             <  SRR4302535.3631768/94‑1 (MQ=60)
                                                                                GTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGC            <  SRR4302535.3100266/94‑1 (MQ=60)
                                                                                 TTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCT           <  SRR4302535.3027827/94‑1 (MQ=60)
                                                                                 TTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCT           <  SRR4302535.1386753/94‑1 (MQ=60)
                                                                                 TTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCT           <  SRR4302535.1814922/94‑1 (MQ=60)
                                                                                     CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT       >  SRR4302535.2376782/1‑94 (MQ=60)
                                                                                     CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT       <  SRR4302535.2656504/94‑1 (MQ=60)
                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG     >  SRR4302535.1284537/1‑94 (MQ=60)
                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG     >  SRR4302535.2949189/1‑94 (MQ=60)
                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG     >  SRR4302535.3192776/1‑94 (MQ=60)
                                                                                        TGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGC    >  SRR4302535.4412186/1‑94 (MQ=60)
                                                                                           AACGACGCGGACTGGAGCTGCGGGGACTTTTGCCGCTGATTTCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA  >  SRR4302535.3975702/1‑94 (MQ=60)
                                                                                           AACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA  >  SRR4302535.3023412/1‑94 (MQ=60)
                                                                                             |                                                                                           
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA  >  NC_000913/4021591‑4021775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑