Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,984	+C	coding (1250/1299 nt)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,983	1	.	C	99.6%	733.1 / ‑4.3	234	coding (1249/1299 nt)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (129/104); total (129/105)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 250 total aligned reads displayed.

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA  >  NC_000913/4031891‑4032069

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCTTTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                      <  2:3011777/93‑3 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                      >  1:4265088/1‑91 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                      <  1:3696627/93‑3 (MQ=255)

     gcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccacg                                                                                    >  1:1955299/1‑89 (MQ=255)

       aCTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACggg                                                                                  <  1:285314/93‑1 (MQ=255)

       aCTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACggg                                                                                  >  2:1115857/1‑93 (MQ=255)

        cTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGc                                                                                 <  1:1287933/93‑1 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                >  2:4298506/1‑93 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCg                                                                                >  2:1890775/1‑93 (MQ=255)

          gCCGGAACGTATCCTGGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGt                                                                               <  2:3706133/93‑1 (MQ=255)

           ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTg                                                                              >  1:149394/1‑93 (MQ=255)

           ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTg                                                                              <  1:3325635/93‑1 (MQ=255)

            cGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTgg                                                                             <  1:533600/93‑1 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGa                                                                            <  1:2047357/93‑1 (MQ=255)

             cgAACGTATCCTCGAAGCCGTTTGGGGAGTTGTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGGGGa                                                                            <  1:3172678/92‑1 (MQ=255)

              gAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAt                                                                           >  2:3531357/1‑93 (MQ=255)

                aCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGa                                                                         >  1:2472962/1‑93 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACt                                                                       >  1:4101993/1‑93 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACt                                                                       >  1:913354/1‑93 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtt                                                                      >  1:3977044/1‑93 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtt                                                                      <  2:1960776/93‑1 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtt                                                                      <  1:1193429/93‑1 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACttt                                                                     >  2:3221992/1‑93 (MQ=255)

                     tCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACtttt                                                                    >  1:3231650/1‑93 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTc                                                                   >  1:1565264/1‑93 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTc                                                                   <  1:600385/93‑1 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTc                                                                   <  1:4475312/93‑1 (MQ=255)

                        tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTg                                                                 >  2:1396880/1‑93 (MQ=255)

                          gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGcc                                                               <  2:378260/93‑1 (MQ=255)

                           aaGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCt                                                              <  2:694293/93‑1 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                             <  1:3900123/93‑1 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                             >  1:834375/1‑93 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCtt                                                             >  2:1225742/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                            >  2:1801234/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                            <  2:3067589/93‑1 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                            >  2:659529/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                            <  2:902453/93‑1 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCttt                                                            <  1:1920388/93‑1 (MQ=255)

                               cGTCTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGc                                                          <  1:3848805/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                         >  1:2828133/1‑93 (MQ=255)

                                 tttGGGGTTTTTTCTCCGCCTATGCGTTGGTGTTTCTTGTCAGTATGCTGGCGATTATCG‑CTACGGGCGGGGCTGACTTTTCTGCCTTTGCGt                                                        >  2:3953885/1‑93 (MQ=255)

                                 tttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                        >  2:1093073/1‑93 (MQ=255)

                                  ttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTc                                                       <  2:1259582/93‑1 (MQ=255)

                                  ttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTc                                                       <  1:688564/93‑1 (MQ=255)

                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                   <  1:2423748/93‑1 (MQ=255)

                                         tttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgttgtt                                                <  2:2289613/93‑1 (MQ=255)

                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                               >  2:2704199/1‑93 (MQ=255)

                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                               >  2:827935/1‑93 (MQ=255)

                                            ttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCg                                             >  1:2119969/1‑93 (MQ=255)

                                            ttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCg                                             <  2:4219007/93‑1 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                          >  1:4571081/1‑93 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                          >  2:3496719/1‑93 (MQ=255)

                                                 cGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAtt                                        <  2:1622363/93‑1 (MQ=255)

                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                       >  1:2950824/1‑93 (MQ=255)

                                                   ccTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                      >  1:4445511/1‑93 (MQ=255)

                                                   catATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGa                                      <  2:555858/91‑1 (MQ=255)

                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                     <  2:2853523/93‑1 (MQ=255)

                                                    cTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGaa                                     >  1:1761295/1‑93 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                    >  2:241814/1‑93 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                    >  1:2083433/1‑93 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTGGTTGCGACATTGAAt                                    <  2:1513324/93‑1 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                   >  2:4440932/1‑93 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                   <  1:2157825/93‑1 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                  >  1:1028508/1‑93 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 <  1:4157474/93‑1 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 <  2:2473358/93‑1 (MQ=255)

                                                        gCATTGATGTTTATTGTCAGTATCCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                 <  2:4081553/93‑1 (MQ=255)

                                                         cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                <  2:3397837/93‑1 (MQ=255)

                                                         cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                >  2:617487/1‑93 (MQ=255)

                                                         cATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTCCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAcc                                <  1:2205903/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                               <  1:1718186/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                               <  1:2994921/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                               <  1:3069316/93‑1 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                              >  1:2731024/1‑93 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                              >  1:4630588/1‑93 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                              <  1:4599815/93‑1 (MQ=255)

                                                             ggTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTggg                            <  1:3049618/93‑1 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                          >  1:3379436/1‑93 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                          <  2:4631426/93‑1 (MQ=255)

                                                                gtTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGcc                         >  2:2934689/1‑93 (MQ=255)

                                                                 tttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCa                        <  1:4004842/93‑1 (MQ=255)

                                                                  ttATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAg                       <  2:1834740/93‑1 (MQ=255)

                                                                  ttATTGTCACTATGCTGGCGATTATCGCC‑ACGGGCGTAGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCAACATTGAATAACCTGGGGCCAg                       <  1:2352199/93‑1 (MQ=255)

                                                                   tATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                      >  1:647353/1‑93 (MQ=255)

                                                                    aTTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAggg                     >  2:2635735/1‑93 (MQ=255)

                                                                    aTTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAggg                     <  2:2046550/93‑1 (MQ=255)

                                                                     ttGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGc                    >  1:232462/1‑93 (MQ=255)

                                                                      tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCt                   <  2:4553039/93‑1 (MQ=255)

                                                                      tGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGAGGCCAGGGCt                   >  1:182535/1‑93 (MQ=255)

                                                                       gTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                  <  2:4196019/93‑1 (MQ=255)

                                                                         cAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTgg                >  1:3539016/1‑93 (MQ=255)

                                                                            tATGCTGGCGATTATCGCC‑ACGGGCGAAAATGACTTTTCTGCCTTTGCATCGATTGTTGCGACATTGAATAAACTGGGGCCAGGGCTTGGCGt             <  1:4680229/93‑1 (MQ=255)

                                                                             aTGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTg            <  1:4206291/93‑1 (MQ=255)

                                                                              tGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgg           >  1:257065/1‑93 (MQ=255)

                                                                                 tGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTCGGGCCAGGGCTTGGCGTGGTTg        >  2:2260596/1‑93 (MQ=255)

                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc       >  1:1428557/1‑93 (MQ=255)

                                                                                  ggCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc       >  1:1066735/1‑93 (MQ=255)

                                                                                      aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt   >  2:3305544/1‑93 (MQ=255)

                                                                                      aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt   >  2:3204455/1‑93 (MQ=255)

                                                                                      aTTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt   >  1:1406917/1‑93 (MQ=255)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA  >  NC_000913/4031891‑4032069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑C                                                                                               <  SRR4302535.3653293/94‑1 (MQ=60)

  CGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑Cca                                                                                             >  SRR4302535.4294296/1‑92 (MQ=60)

   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑Ccac                                                                                            <  SRR4302535.3722209/94‑4 (MQ=60)

    CGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACG                                                                                            >  SRR4302535.1969375/1‑94 (MQ=60)

    CGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACG                                                                                            >  SRR4302535.2434069/1‑94 (MQ=60)

     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGG                                                                                           >  SRR4302535.540575/1‑94 (MQ=60)

     GCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGG                                                                                           >  SRR4302535.3584365/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGG                                                                                          >  SRR4302535.1113654/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGG                                                                                          >  SRR4302535.835155/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGG                                                                                          >  SRR4302535.716497/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGG                                                                                          <  SRR4302535.3502881/94‑1 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGC                                                                                         >  SRR4302535.4022739/1‑94 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGC                                                                                         <  SRR4302535.287307/94‑1 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGC                                                                                         <  SRR4302535.1478446/94‑1 (MQ=60)

       ACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑Ccaccggg                                                                                        >  SRR4302535.515667/1‑87 (MQ=60)

        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCG                                                                                        <  SRR4302535.2152079/94‑1 (MQ=60)

        CTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCG                                                                                        <  SRR4302535.1297111/94‑1 (MQ=60)

          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTG                                                                                      >  SRR4302535.150433/1‑94 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGG                                                                                     <  SRR4302535.3348778/94‑1 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGG                                                                                     <  SRR4302535.3934960/94‑1 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGA                                                                                    <  SRR4302535.537334/94‑1 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGAT                                                                                   <  SRR4302535.2062098/94‑1 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGAT                                                                                   <  SRR4302535.3194641/94‑1 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGAT                                                                                   >  SRR4302535.4185966/1‑94 (MQ=60)

             CGAACGTATCCTCGAAGCCGTTTGGGGAGTTGTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGGGGAT                                                                                   <  SRR4302535.3194640/94‑1 (MQ=60)

              GAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATG                                                                                  <  SRR4302535.4347673/94‑1 (MQ=60)

               AACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGA                                                                                 >  SRR4302535.2490797/1‑94 (MQ=60)

               AACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGA                                                                                 >  SRR4302535.2086029/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACT                                                                               <  SRR4302535.1832240/94‑1 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACT                                                                               >  SRR4302535.4130078/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACT                                                                               >  SRR4302535.1438409/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACT                                                                               >  SRR4302535.919845/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTT                                                                              >  SRR4302535.4004215/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTT                                                                              >  SRR4302535.4086360/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTT                                                                              >  SRR4302535.953918/1‑94 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTT                                                                             <  SRR4302535.1201981/94‑1 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTT                                                                             >  SRR4302535.3777535/1‑94 (MQ=60)

                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTT                                                                            >  SRR4302535.3254058/1‑94 (MQ=60)

                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTT                                                                            >  SRR4302535.2584327/1‑94 (MQ=60)

                     TCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTC                                                                           >  SRR4302535.1576566/1‑94 (MQ=60)

                     TCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTC                                                                           >  SRR4302535.4541528/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCT                                                                          >  SRR4302535.160398/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302535.4506019/94‑1 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302535.604585/94‑1 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTG                                                                         <  SRR4302535.3331867/94‑1 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTG                                                                         <  SRR4302535.1491589/94‑1 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTG                                                                         <  SRR4302535.2112063/94‑1 (MQ=60)

                        TCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGC                                                                        >  SRR4302535.2541857/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCC                                                                       <  SRR4302535.1944929/94‑1 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302535.2734610/1‑94 (MQ=60)

                           AAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTT                                                                     >  SRR4302535.840227/1‑94 (MQ=60)

                           AAGCCGTTTGGGGATTTTCCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTT                                                                     <  SRR4302535.453096/94‑1 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302535.3926838/94‑1 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302535.3058191/94‑1 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302535.3528448/94‑1 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302535.63742/94‑1 (MQ=60)

                             GCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   <  SRR4302535.1934232/94‑1 (MQ=60)

                             GCCGTTTGAGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTG                                                                   <  SRR4302535.4236459/94‑1 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302535.2848203/1‑94 (MQ=60)

                               CGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 >  SRR4302535.1252870/1‑94 (MQ=60)

                               CGTCTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCG                                                                 <  SRR4302535.3875294/94‑1 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               <  SRR4302535.483130/94‑1 (MQ=60)

                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              >  SRR4302535.3156025/1‑94 (MQ=60)

                                  TTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCG                                                              <  SRR4302535.693387/94‑1 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          <  SRR4302535.2441212/94‑1 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        >  SRR4302535.3917684/1‑94 (MQ=60)

                                        ATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTT                                                        <  SRR4302535.2138304/94‑1 (MQ=60)

                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCCGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGGTGTTG                                                       >  SRR4302535.2780098/1‑94 (MQ=60)

                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       <  SRR4302535.1819431/94‑1 (MQ=60)

                                           TTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCG                                                     >  SRR4302535.2135259/1‑94 (MQ=60)

                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAC                                                   <  SRR4302535.4624363/94‑1 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  >  SRR4302535.4417667/1‑94 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  >  SRR4302535.4602247/1‑94 (MQ=60)

                                              CTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACA                                                  >  SRR4302535.3771269/1‑94 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302535.400377/94‑1 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302535.2290335/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               <  SRR4302535.1351669/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               >  SRR4302535.2971553/1‑94 (MQ=60)

                                                  GCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGA                                              >  SRR4302535.4475998/1‑94 (MQ=60)

                                                  GCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGA                                              >  SRR4302535.1948927/1‑94 (MQ=60)

                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             >  SRR4302535.3003726/1‑94 (MQ=60)

                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             >  SRR4302535.1774051/1‑94 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            >  SRR4302535.2947993/1‑94 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            >  SRR4302535.2098438/1‑94 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            >  SRR4302535.207660/1‑94 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302535.1715057/1‑94 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302535.2173333/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302535.1035811/1‑94 (MQ=60)

                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        <  SRR4302535.4185934/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302535.2274913/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302535.4639869/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTCCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       <  SRR4302535.2221613/94‑1 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      <  SRR4302535.1730616/94‑1 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302535.4662006/1‑94 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302535.2405549/1‑94 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302535.2750521/1‑94 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      <  SRR4302535.3015917/94‑1 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      <  SRR4302535.3090753/94‑1 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302535.4631101/94‑1 (MQ=60)

                                                           TTGGTGTTTAGTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302535.643911/94‑1 (MQ=60)

                                                            TGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    >  SRR4302535.4635910/1‑94 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302535.4133587/94‑1 (MQ=60)

                                                             GGTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGG                                   <  SRR4302535.3070982/94‑1 (MQ=60)

                                                              GTGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  >  SRR4302535.3402933/1‑94 (MQ=60)

                                                               TGTTTATTGTCAGTATGCTGGCGATTATCCC‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCC                                 <  SRR4302535.2038611/94‑1 (MQ=60)

                                                               TGTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCC                                 >  SRR4302535.2357360/1‑94 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                <  SRR4302535.3098179/94‑1 (MQ=60)

                                                                 TTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATaaagaaggaggtttccat                               <  SRR4302535.2768109/94‑19 (MQ=60)

                                                                 TTTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAG                               <  SRR4302535.4032237/94‑1 (MQ=60)

                                                                  TTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              <  SRR4302535.1074263/94‑1 (MQ=60)

                                                                  TTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              >  SRR4302535.651864/1‑94 (MQ=60)

                                                                  TTATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              >  SRR4302535.1567277/1‑94 (MQ=60)

                                                                  TTATTGTCACTATGCTGGCGATTAT‑CGCCACGGGCGTAGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCAACATTGAATAACCTGGGGCCAGG                              <  SRR4302535.2369063/94‑1 (MQ=60)

                                                                   TATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGG                             >  SRR4302535.1092316/1‑94 (MQ=60)

                                                                   TATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGG                             >  SRR4302535.4233086/1‑94 (MQ=60)

                                                                    ATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302535.234066/1‑94 (MQ=60)

                                                                    ATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302535.529050/1‑94 (MQ=60)

                                                                    ATTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302535.3396374/1‑94 (MQ=60)

                                                                    ATTGTCAGTATGCTGGCGATTAT‑AGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGC                            >  SRR4302535.1394327/1‑94 (MQ=60)

                                                                     tgcTCAGTATGCTGGCGATTAT‑CGCCACGGGGGTGGATGACTTTTCTGCCTTTGCGGCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCT                           <  SRR4302535.293832/91‑1 (MQ=60)

                                                                     TTGTCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGAGGCCAGGGCT                           >  SRR4302535.183795/1‑94 (MQ=60)

                                                                        TCAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGG                        >  SRR4302535.3563584/1‑94 (MQ=60)

                                                                         CAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       >  SRR4302535.685943/1‑94 (MQ=60)

                                                                         CAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       >  SRR4302535.3563258/1‑94 (MQ=60)

                                                                         CAGTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGC                       <  SRR4302535.1873886/94‑1 (MQ=60)

                                                                           GTATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGT                     >  SRR4302535.2864653/1‑94 (MQ=60)

                                                                            TATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    >  SRR4302535.866349/1‑94 (MQ=60)

                                                                            TATGCTGGCGATTAT‑CGCCACGGGCGAAAATGACTTTTCTGCCTTTGCATCGATTGTTGCGACATTGAATAAACTGGGGCCAGGGCTTGGCGTG                    <  SRR4302535.4711835/94‑1 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302535.4101365/1‑94 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302535.551563/1‑94 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   <  SRR4302535.4235076/94‑1 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302535.258840/1‑94 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302535.2445089/1‑94 (MQ=60)

                                                                             ATGCTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGG                   >  SRR4302535.3087214/1‑94 (MQ=60)

                                                                                CTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                <  SRR4302535.4676062/94‑1 (MQ=60)

                                                                                CTGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCGTGGCGTGGTTG                >  SRR4302535.2758053/1‑94 (MQ=60)

                                                                                 TGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGC               >  SRR4302535.1438750/1‑94 (MQ=60)

                                                                                 TGGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGC               >  SRR4302535.1074321/1‑94 (MQ=60)

                                                                                  GGCGATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              >  SRR4302535.3055240/1‑94 (MQ=60)

                                                                                     GATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302535.1764519/1‑94 (MQ=60)

                                                                                     GATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302535.1416970/1‑94 (MQ=60)

                                                                                     GATTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302535.4501076/1‑94 (MQ=60)

                                                                                       TTAT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAA         <  SRR4302535.2208093/94‑1 (MQ=60)

                                                                                         AT‑CGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACT       <  SRR4302535.1960755/94‑1 (MQ=60)

                                                                                            cgcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     >  SRR4302535.4185079/4‑94 (MQ=60)

                                                                                             gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302535.2199294/3‑94 (MQ=60)

                                                                                             gcCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    >  SRR4302535.877452/3‑94 (MQ=60)

                                                                                             C‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTAC   >  SRR4302535.2687445/1‑94 (MQ=60)

                                                                                               CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  <  SRR4302535.4581407/94‑1 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTAT‑CG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`