Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,043,629	A→G	I185T (ATT→ACT)	yihG ←	putative endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,629	0	A	G	99.6%	832.3 / ‑5.0	262	I185T (ATT→ACT)	yihG	putative endonuclease
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (114/147); total (115/147)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

Only 100 of 275 total aligned reads displayed.

GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA  >  NC_000913/4043539‑4043718

 ccgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtgg                                                                                        <  2:2973186/93‑1 (MQ=255)

  cgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggt                                                                                       <  1:2154645/93‑1 (MQ=255)

    ccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtgg                                                                                     >  1:4634996/1‑93 (MQ=255)

     cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  2:4457845/93‑1 (MQ=255)

     cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  1:1790208/93‑1 (MQ=255)

     cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  2:1884727/93‑1 (MQ=255)

     cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGCTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  1:1487338/93‑1 (MQ=255)

      tttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtggtg                                                                                   >  1:4564476/1‑93 (MQ=255)

         ggtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGa                                                                                <  2:1234574/93‑1 (MQ=255)

          gtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAt                                                                               <  2:545321/93‑1 (MQ=255)

          gtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAt                                                                               >  2:1719419/1‑93 (MQ=255)

           tggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATg                                                                              >  1:2236884/1‑93 (MQ=255)

           tggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATg                                                                              >  1:2822124/1‑93 (MQ=255)

             gCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCa                                                                            <  1:3783783/93‑1 (MQ=255)

              caacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAg                                                                           >  2:3341056/1‑93 (MQ=255)

              caacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAg                                                                           <  1:697459/93‑1 (MQ=255)

              caacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAg                                                                           <  1:834574/93‑1 (MQ=255)

               aacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAgg                                                                          >  2:1834252/1‑93 (MQ=255)

                acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         >  1:4080415/1‑93 (MQ=255)

                acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         <  2:1655936/93‑1 (MQ=255)

                acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         >  2:4025917/1‑93 (MQ=255)

                acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         >  2:4421379/1‑93 (MQ=255)

                  aaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGa                                                                       >  2:4636919/1‑93 (MQ=255)

                  aaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGa                                                                       >  1:3170883/1‑93 (MQ=255)

                     ttttGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAAc                                                                    >  2:792044/1‑93 (MQ=255)

                     ttttGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAAc                                                                    <  2:3538208/93‑1 (MQ=255)

                      tttGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACt                                                                   >  2:666661/1‑93 (MQ=255)

                        tGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACttt                                                                 <  1:682725/93‑1 (MQ=255)

                         gAAAAGAGGAGTGGGGTTGCTGATGTTTTTCTTGCGTGAAACGGGGGCGTTCAACGGAATTGACAGAGGTGGTGGGACGCACGCGAGACtgtt                                                                >  2:4000066/1‑93 (MQ=255)

                           aaaGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTca                                                              >  2:233252/1‑93 (MQ=255)

                           aaaGTGGAGGGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCGTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTca                                                              >  2:4558450/1‑93 (MQ=255)

                             agtggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTcaca                                                            <  1:1025066/93‑1 (MQ=255)

                              gtggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACaa                                                           >  2:2495941/1‑93 (MQ=255)

                               tggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAg                                                          >  1:2103488/1‑93 (MQ=255)

                                ggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGa                                                         <  1:654389/93‑1 (MQ=255)

                                 gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        >  2:3596982/1‑93 (MQ=255)

                                 gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        <  2:3015920/93‑1 (MQ=255)

                                 gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        <  1:1660758/93‑1 (MQ=255)

                                  agtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAc                                                       <  1:3267598/93‑1 (MQ=255)

                                   gtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACg                                                      >  2:3216806/1‑93 (MQ=255)

                                   gtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACg                                                      >  1:3077619/1‑93 (MQ=255)

                                    tggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGc                                                     <  1:1193320/93‑1 (MQ=255)

                                    tggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGc                                                     >  2:3169297/1‑93 (MQ=255)

                                    tggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGc                                                     <  1:2814981/93‑1 (MQ=255)

                                        tttGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAg                                                 <  2:140824/93‑1 (MQ=255)

                                         ttGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGt                                                <  2:2401144/93‑1 (MQ=255)

                                          tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTg                                               <  1:3881644/93‑1 (MQ=255)

                                          tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTg                                               <  2:27853/93‑1 (MQ=255)

                                          tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTg                                               >  2:2861007/1‑93 (MQ=255)

                                           gCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTgg                                              >  2:2112839/1‑93 (MQ=255)

                                            cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGGAGGCGAAACTTTTCACAAGAACACCGAggcgt                                             >  2:3946293/1‑89 (MQ=255)

                                            cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGt                                             >  1:532563/1‑93 (MQ=255)

                                            cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGt                                             <  2:3731631/93‑1 (MQ=255)

                                            cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGt                                             >  1:3224919/1‑93 (MQ=255)

                                             tgatgaTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGtt                                            >  2:3054909/1‑93 (MQ=255)

                                              gATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGTGTGGttt                                           >  1:569255/1‑93 (MQ=255)

                                              gATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGttt                                           <  2:2004569/93‑1 (MQ=255)

                                                tGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCa                                         >  1:1080676/1‑93 (MQ=255)

                                                tGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCa                                         <  2:243378/93‑1 (MQ=255)

                                                tGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCa                                         >  1:1368242/1‑93 (MQ=255)

                                                 gTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCaa                                        <  1:2627535/93‑1 (MQ=255)

                                                  tttttCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAc                                       >  2:3904210/1‑93 (MQ=255)

                                                  tttttCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAc                                       >  1:2382848/1‑93 (MQ=255)

                                                  ttgttCGTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAc                                       <  2:1359228/90‑1 (MQ=255)

                                                     ttCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATc                                    <  1:293645/93‑1 (MQ=255)

                                                      tcgtGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGAAGGCGAAACTTTTCACAAGAACGCCGAGTGGGTTCAACATCt                                   >  2:3095717/4‑93 (MQ=255)

                                                      tCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCt                                   >  1:3349853/1‑93 (MQ=255)

                                                        ttGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACACGAACGCCGAGTGGTTTCAACATCttt                                 >  1:416059/1‑93 (MQ=255)

                                                        ttGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCttt                                 <  2:2905447/93‑1 (MQ=255)

                                                         tGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGTCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTa                                <  1:3302769/93‑1 (MQ=255)

                                                          gCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAc                               <  1:31772/93‑1 (MQ=255)

                                                          gCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAc                               <  2:3677547/93‑1 (MQ=255)

                                                          gCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAc                               <  1:1845835/93‑1 (MQ=255)

                                                               aaaCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGt                          <  2:4678011/93‑1 (MQ=255)

                                                                aaCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTc                         <  2:4329313/93‑1 (MQ=255)

                                                                  cGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGc                       <  1:2167041/93‑1 (MQ=255)

                                                                   gggAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCt                      <  2:2695730/93‑1 (MQ=255)

                                                                    ggAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTc                     >  1:3438882/1‑93 (MQ=255)

                                                                     gAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTcc                    <  1:4271817/93‑1 (MQ=255)

                                                                       gCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCgg                  <  2:1491720/93‑1 (MQ=255)

                                                                        ccTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGa                 >  2:612918/1‑93 (MQ=255)

                                                                        ccTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGa                 <  2:406996/93‑1 (MQ=255)

                                                                        ccTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAAAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGa                 <  1:3632564/93‑1 (MQ=255)

                                                                         cTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAt                <  1:3969734/93‑1 (MQ=255)

                                                                         cTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAt                <  1:2014682/93‑1 (MQ=255)

                                                                         cTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAt                >  1:112875/1‑93 (MQ=255)

                                                                          ttCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATg               <  1:1888068/93‑1 (MQ=255)

                                                                           tCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGa              <  1:3945935/93‑1 (MQ=255)

                                                                           tCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGa              >  2:4307630/1‑93 (MQ=255)

                                                                           tCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGa              <  2:2208618/93‑1 (MQ=255)

                                                                            aaaCGAAATCGACAGTGGTGGTGGTATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTAAACATCTTTACCACGTCGCTCCGGATgtc             <  2:844205/92‑3 (MQ=255)

                                                                             aaCGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACg            >  1:4456340/1‑93 (MQ=255)

                                                                              aCGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCATGTCGCTCCGGATGACGt           >  2:1275233/1‑93 (MQ=255)

                                                                                 aaaTTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGtaat        >  2:384965/1‑93 (MQ=255)

                                                                                   aTTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTGAACATCTTTACCACGTCGCTCCGGATGACGtaataa      <  1:2602132/93‑1 (MQ=255)

                                                                                    ttGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACACCTTTACCACGCCGCTCCGGATGACGTaataaa     <  2:2564608/93‑1 (MQ=255)

                                                                                     tGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaat    <  2:3291062/93‑1 (MQ=255)

                                                                                     tGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaat    <  1:1565062/93‑1 (MQ=255)

                                                                                      gACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaata   <  2:1029397/93‑1 (MQ=255)

                                                                                       aCAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAataaataa  <  1:2278854/93‑1 (MQ=255)

GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA  >  NC_000913/4043539‑4043718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCGC  >  NC_000913/4043536‑4043722

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAG                                                                                               <  SRR4302535.3103615/94‑1 (MQ=60)

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAG                                                                                               <  SRR4302535.662724/94‑1 (MQ=60)

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAG                                                                                               <  SRR4302535.4712640/94‑1 (MQ=60)

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAG                                                                                               <  SRR4302535.3979219/94‑1 (MQ=60)

 CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                              <  SRR4302535.4434041/94‑1 (MQ=60)

 CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                              >  SRR4302535.752836/1‑94 (MQ=60)

   GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGG                                                                                            >  SRR4302535.1919671/1‑94 (MQ=60)

     CGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTG                                                                                          <  SRR4302535.688425/94‑1 (MQ=60)

     CGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTG                                                                                          <  SRR4302535.2170128/94‑1 (MQ=60)

      GCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGG                                                                                         >  SRR4302535.4666434/1‑94 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGT                                                                                        >  SRR4302535.1189472/1‑94 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGT                                                                                        >  SRR4302535.2080359/1‑94 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGT                                                                                        >  SRR4302535.2790653/1‑94 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACA‑‑‑GTGGTGGG                                                                                     >  SRR4302535.4204613/1‑94 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                       >  SRR4302535.552587/1‑94 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                       >  SRR4302535.4595622/1‑94 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                       <  SRR4302535.1803156/94‑1 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGCTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                       <  SRR4302535.1497991/94‑1 (MQ=60)

         TTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGG                                                                                      <  SRR4302535.544688/94‑1 (MQ=60)

           TGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGA                                                                                    >  SRR4302535.2523/1‑94 (MQ=60)

            GGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAT                                                                                   <  SRR4302535.3726005/94‑1 (MQ=60)

             GTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATG                                                                                  >  SRR4302535.2252796/1‑94 (MQ=60)

             GTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATG                                                                                  >  SRR4302535.2842161/1‑94 (MQ=60)

              TGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGC                                                                                 >  SRR4302535.4032725/1‑94 (MQ=60)

               GGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCA                                                                                <  SRR4302535.3330847/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAG                                                                               <  SRR4302535.3809890/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAG                                                                               <  SRR4302535.4413187/94‑1 (MQ=60)

                 CAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGG                                                                              <  SRR4302535.496128/94‑1 (MQ=60)

                 CAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGG                                                                              <  SRR4302535.840427/94‑1 (MQ=60)

                 CAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGG                                                                              <  SRR4302535.4631890/94‑1 (MQ=60)

                 CAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGG                                                                              <  SRR4302535.702353/94‑1 (MQ=60)

                  AACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGC                                                                             >  SRR4302535.3835098/1‑94 (MQ=60)

                  AACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGC                                                                             >  SRR4302535.4108325/1‑94 (MQ=60)

                   ACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCG                                                                            <  SRR4302535.1883591/94‑1 (MQ=60)

                    CAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGA                                                                           >  SRR4302535.576554/1‑94 (MQ=60)

                    CAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGA                                                                           >  SRR4302535.3932971/1‑94 (MQ=60)

                    CAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGA                                                                           >  SRR4302535.3192840/1‑94 (MQ=60)

                           TGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTT                                                                    >  SRR4302535.3291333/1‑94 (MQ=60)

                           TGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTT                                                                    <  SRR4302535.3981587/94‑1 (MQ=60)

                           TGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTT                                                                    <  SRR4302535.687505/94‑1 (MQ=60)

                            GAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTC                                                                   >  SRR4302535.357757/1‑94 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                  >  SRR4302535.2602735/1‑94 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                  <  SRR4302535.2173739/94‑1 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                  >  SRR4302535.2193479/1‑94 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                  <  SRR4302535.1287208/94‑1 (MQ=60)

                              AAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCAC                                                                 >  SRR4302535.1599134/1‑94 (MQ=60)

                               AAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACA                                                                >  SRR4302535.4031323/1‑94 (MQ=60)

                                AGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAA                                                               <  SRR4302535.1032349/94‑1 (MQ=60)

                                 GTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAG                                                              <  SRR4302535.1467156/94‑1 (MQ=60)

                                 GTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAG                                                              >  SRR4302535.2118659/1‑94 (MQ=60)

                                  TGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGA                                                             >  SRR4302535.3164919/1‑94 (MQ=60)

                                   GGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAA                                                            >  SRR4302535.3095902/1‑94 (MQ=60)

                                   GGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAA                                                            <  SRR4302535.658959/94‑1 (MQ=60)

                                   GGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAA                                                            >  SRR4302535.362335/1‑94 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                           <  SRR4302535.1672759/94‑1 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                           <  SRR4302535.4296338/94‑1 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACG                                                          <  SRR4302535.3290296/94‑1 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACG                                                          >  SRR4302535.1687233/1‑94 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACG                                                          >  SRR4302535.3099088/1‑94 (MQ=60)

                                       TGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCC                                                        <  SRR4302535.1201870/94‑1 (MQ=60)

                                       TGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCC                                                        <  SRR4302535.4532050/94‑1 (MQ=60)

                                       TGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCC                                                        <  SRR4302535.2834981/94‑1 (MQ=60)

                                       TGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCC                                                        >  SRR4302535.2173881/1‑94 (MQ=60)

                                        GGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCG                                                       <  SRR4302535.2258846/94‑1 (MQ=60)

                                         GGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGA                                                      <  SRR4302535.996022/94‑1 (MQ=60)

                                          GTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAG                                                     <  SRR4302535.1100710/94‑1 (MQ=60)

                                           TTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGT                                                    <  SRR4302535.700105/94‑1 (MQ=60)

                                            TTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGAGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGAG                                                   >  SRR4302535.2984709/1‑94 (MQ=60)

                                             TGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGG                                                  <  SRR4302535.3908280/94‑1 (MQ=60)

                                              GCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGT                                                 >  SRR4302535.3247274/1‑94 (MQ=60)

                                              GCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGT                                                 >  SRR4302535.536290/1‑94 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                                <  SRR4302535.2074450/94‑1 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                                <  SRR4302535.3417879/94‑1 (MQ=60)

                                                TGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGTGTGGTTT                                               >  SRR4302535.573252/1‑94 (MQ=60)

                                                TGATGTTTTGCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTT                                               <  SRR4302535.1115791/94‑1 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCA                                             >  SRR4302535.1088364/1‑94 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCA                                             >  SRR4302535.1378019/1‑94 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCA                                             >  SRR4302535.2956788/1‑94 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCA                                             >  SRR4302535.1849957/1‑94 (MQ=60)

                                                    GTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAC                                           <  SRR4302535.2646366/94‑1 (MQ=60)

                                                    GTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAC                                           >  SRR4302535.4154233/1‑94 (MQ=60)

                                                    GTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAC                                           >  SRR4302535.2399960/1‑94 (MQ=60)

                                                    GTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAAC                                           <  SRR4302535.3983855/94‑1 (MQ=60)

                                                     TTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACA                                          >  SRR4302535.887660/1‑94 (MQ=60)

                                                      TTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACAT                                         <  SRR4302535.491640/94‑1 (MQ=60)

                                                       TTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATC                                        >  SRR4302535.1552420/1‑94 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                       >  SRR4302535.3373187/1‑94 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                       <  SRR4302535.1889806/94‑1 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                       <  SRR4302535.295697/94‑1 (MQ=60)

                                                         TCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTT                                      <  SRR4302535.677674/94‑1 (MQ=60)

                                                          CTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACACGAACGCCGAGTGGTTTCAACATCTTT                                     >  SRR4302535.418975/1‑94 (MQ=60)

                                                           TTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTA                                    <  SRR4302535.2836944/94‑1 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGTCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAC                                   <  SRR4302535.3325735/94‑1 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAC                                   >  SRR4302535.3430827/1‑94 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAC                                   >  SRR4302535.32767/1‑94 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGAGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACAGCTTTGC                                   >  SRR4302535.705790/1‑94 (MQ=60)

                                                             GCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACC                                  <  SRR4302535.1859136/94‑1 (MQ=60)

                                                             GCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACC                                  <  SRR4302535.32016/94‑1 (MQ=60)

                                                               GTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCAC                                >  SRR4302535.624562/1‑94 (MQ=60)

                                                                TGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACG                               >  SRR4302535.1332523/1‑94 (MQ=60)

                                                                   AACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACACGAACGCCGAGTGGTTTCACCATCTTTACCACGTCG                            >  SRR4302535.2271645/1‑94 (MQ=60)

                                                                   AACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCG                            <  SRR4302535.3958412/94‑1 (MQ=60)

                                                                     CGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCT                          <  SRR4302535.3197670/94‑1 (MQ=60)

                                                                     CGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCT                          <  SRR4302535.2182574/94‑1 (MQ=60)

                                                                     CGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCT                          <  SRR4302535.2911855/94‑1 (MQ=60)

                                                                      GGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTC                         >  SRR4302535.3462800/1‑94 (MQ=60)

                                                                      GGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTC                         <  SRR4302535.2648423/94‑1 (MQ=60)

                                                                      GGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTC                         >  SRR4302535.1655804/1‑94 (MQ=60)

                                                                        GAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCG                       <  SRR4302535.4301062/94‑1 (MQ=60)

                                                                         AGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGG                      <  SRR4302535.2630324/94‑1 (MQ=60)

                                                                         AGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGG                      >  SRR4302535.1571502/1‑94 (MQ=60)

                                                                         AGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGG                      >  SRR4302535.4568543/1‑94 (MQ=60)

                                                                           CCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAT                    >  SRR4302535.113666/1‑94 (MQ=60)

                                                                           CCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAAAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAT                    <  SRR4302535.3657740/94‑1 (MQ=60)

                                                                            CTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATG                   <  SRR4302535.3996850/94‑1 (MQ=60)

                                                                            CTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATG                   <  SRR4302535.2029194/94‑1 (MQ=60)

                                                                             TTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGA                  <  SRR4302535.1901668/94‑1 (MQ=60)

                                                                             TTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGA                  >  SRR4302535.2161497/1‑94 (MQ=60)

                                                                             TTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGA                  <  SRR4302535.2308119/94‑1 (MQ=60)

                                                                              TCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGAC                 <  SRR4302535.3241731/94‑1 (MQ=60)

                                                                              TCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGAC                 <  SRR4302535.3972881/94‑1 (MQ=60)

                                                                              TCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGAC                 <  SRR4302535.2192828/94‑1 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG                >  SRR4302535.4486901/1‑94 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG                <  SRR4302535.768139/94‑1 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG                >  SRR4302535.3265280/1‑94 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG                <  SRR4302535.3681480/94‑1 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT               >  SRR4302535.1059784/1‑94 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT               >  SRR4302535.3243621/1‑94 (MQ=60)

                                                                                 ACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTA              >  SRR4302535.3186271/1‑94 (MQ=60)

                                                                                  CGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAA             >  SRR4302535.1940203/1‑94 (MQ=60)

                                                                                  CGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAA             >  SRR4302535.264164/1‑94 (MQ=60)

                                                                                  CGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAA             <  SRR4302535.4463822/94‑1 (MQ=60)

                                                                                    AAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATA           <  SRR4302535.1159267/94‑1 (MQ=60)

                                                                                      ATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTGAACATCTTTACCACGTCGCTCCGGATGACGTAATAAA         <  SRR4302535.2620812/94‑1 (MQ=60)

                                                                                        TGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATA       <  SRR4302535.1735332/94‑1 (MQ=60)

                                                                                        TGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATA       <  SRR4302535.1576362/94‑1 (MQ=60)

                                                                                        TGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATA       <  SRR4302535.3291033/94‑1 (MQ=60)

                                                                                          ACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAG     >  SRR4302535.2193496/1‑94 (MQ=60)

                                                                                          ACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAG     <  SRR4302535.2295131/94‑1 (MQ=60)

                                                                                            AGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCG   <  SRR4302535.258595/94‑1 (MQ=60)

                                                                                             GTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCGC  >  SRR4302535.4092259/1‑94 (MQ=60)

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCGC  >  NC_000913/4043536‑4043722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`