Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,045,192 C→T A216V (GCC→GTC)  polA → DNA polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,045,1920CT100.0% 774.7 / NA 215A216V (GCC→GTC) polADNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (102/113);  total (102/113)

Only 100 of 221 total aligned reads displayed.

CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281
                                                                                          |                                                                                         
 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        <  1:904909/93‑1 (MQ=255)
  tctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAg                                                                                       >  2:496696/1‑93 (MQ=255)
  tctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGAATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAg                                                                                       <  1:4451124/93‑1 (MQ=255)
    tGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGcc                                                                                     >  2:81915/1‑93 (MQ=255)
     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  1:1435102/1‑93 (MQ=255)
     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  2:1889597/1‑93 (MQ=255)
     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  1:2329904/1‑93 (MQ=255)
      aTAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAg                                                                                   <  1:3593042/93‑1 (MQ=255)
       tAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGa                                                                                  >  2:555358/1‑93 (MQ=255)
          cATTCCGGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaa                                                                               <  2:423571/93‑1 (MQ=255)
           aTTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaa                                                                              >  1:454837/1‑93 (MQ=255)
            ttCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaaa                                                                             >  1:3241543/1‑93 (MQ=255)
             tCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAAt                                                                            >  2:1286189/1‑93 (MQ=255)
                tGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGc                                                                         <  2:297853/93‑1 (MQ=255)
                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:2029422/93‑1 (MQ=255)
                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:517596/93‑1 (MQ=255)
                    gTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTggg                                                                     >  1:2455893/1‑93 (MQ=255)
                      aCCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGtt                                                                   >  1:2846708/1‑93 (MQ=255)
                      aCCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGtt                                                                   <  2:1563169/93‑1 (MQ=255)
                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  2:905306/93‑1 (MQ=255)
                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  1:2638334/93‑1 (MQ=255)
                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  1:2507605/93‑1 (MQ=255)
                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              >  1:1343962/1‑93 (MQ=255)
                             gTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTc                                                            <  2:1943707/93‑1 (MQ=255)
                             gTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTc                                                            >  1:4087064/1‑93 (MQ=255)
                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           >  2:1353053/1‑93 (MQ=255)
                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           <  2:2546324/93‑1 (MQ=255)
                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGCTGAGCTTcc                                                           >  2:691790/1‑93 (MQ=255)
                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         >  2:1754148/1‑93 (MQ=255)
                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         >  1:2626709/1‑93 (MQ=255)
                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         <  2:4395521/93‑1 (MQ=255)
                                 gTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTg                                                        <  1:3523718/93‑1 (MQ=255)
                                   gAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgc                                                      >  1:320664/1‑93 (MQ=255)
                                    aaaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcg                                                     >  2:1162430/1‑93 (MQ=255)
                                     aaaaaCCGCGTAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    <  2:1237686/93‑1 (MQ=255)
                                     aaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    >  1:108545/1‑93 (MQ=255)
                                     aaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    >  2:3445841/1‑93 (MQ=255)
                                       aaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGa                                                  >  1:4128429/1‑93 (MQ=255)
                                           cgcgCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAAc                                              <  1:279131/93‑1 (MQ=255)
                                             cgcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGTGCTTCCGTGGCGCGAAAACaa                                            <  2:2983772/93‑1 (MQ=255)
                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           >  2:2694809/1‑93 (MQ=255)
                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           <  2:3261758/93‑1 (MQ=255)
                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           >  1:3891993/1‑93 (MQ=255)
                                               cAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATg                                          >  1:56642/1‑93 (MQ=255)
                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        >  1:2006631/1‑93 (MQ=255)
                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        <  2:3924692/93‑1 (MQ=255)
                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        >  1:1786399/1‑93 (MQ=255)
                                                  gCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCa                                       <  2:652987/93‑1 (MQ=255)
                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     >  2:1206821/1‑93 (MQ=255)
                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     <  1:2903699/93‑1 (MQ=255)
                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     >  1:1150957/1‑93 (MQ=255)
                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     <  2:2030081/93‑1 (MQ=255)
                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCg                                    >  2:723570/1‑93 (MQ=255)
                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:107673/93‑1 (MQ=255)
                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  >  2:2513543/1‑93 (MQ=255)
                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:2136880/93‑1 (MQ=255)
                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:4431426/93‑1 (MQ=255)
                                                        ctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAg                                 <  2:3904674/93‑1 (MQ=255)
                                                         tgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGc                                <  1:2197104/93‑1 (MQ=255)
                                                          gcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCt                               >  2:27334/1‑93 (MQ=255)
                                                          gcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCt                               >  1:3600884/1‑93 (MQ=255)
                                                           cAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTc                              >  1:335270/1‑93 (MQ=255)
                                                              ggTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAg                           <  2:4149170/93‑1 (MQ=255)
                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  1:2702343/93‑1 (MQ=255)
                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  2:4631621/93‑1 (MQ=255)
                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:2477741/93‑1 (MQ=255)
                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:1508100/93‑1 (MQ=255)
                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:4160297/93‑1 (MQ=255)
                                                                    ggCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAc                     <  2:1813039/93‑1 (MQ=255)
                                                                     gCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACa                    <  2:2991147/93‑1 (MQ=255)
                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  <  2:3843605/93‑1 (MQ=255)
                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  <  2:3698682/93‑1 (MQ=255)
                                                                        gACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaag                 >  1:4659130/1‑93 (MQ=255)
                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  2:217238/93‑1 (MQ=255)
                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  2:1845880/93‑1 (MQ=255)
                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  1:4417092/93‑1 (MQ=255)
                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  1:10376/93‑1 (MQ=255)
                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  1:620081/93‑1 (MQ=255)
                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               >  1:3731959/1‑93 (MQ=255)
                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTACGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  2:2949735/93‑1 (MQ=255)
                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              >  1:1669951/1‑93 (MQ=255)
                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  2:2455544/93‑1 (MQ=255)
                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  2:1149255/93‑1 (MQ=255)
                                                                            ggATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGt             <  1:3711011/93‑1 (MQ=255)
                                                                            ggATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGt             >  1:4593341/1‑93 (MQ=255)
                                                                             gATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGtt            <  1:1536066/93‑1 (MQ=255)
                                                                              aTACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTg           >  2:4119884/1‑93 (MQ=255)
                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          >  2:491545/1‑93 (MQ=255)
                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          >  2:223742/1‑93 (MQ=255)
                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          <  1:306133/93‑1 (MQ=255)
                                                                                aCGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCt         <  1:3984079/93‑1 (MQ=255)
                                                                                   cTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAt      >  1:3127757/1‑93 (MQ=255)
                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     >  2:2106019/1‑93 (MQ=255)
                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   >  2:2276417/1‑93 (MQ=255)
                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  1:195078/93‑1 (MQ=255)
                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  1:1176956/93‑1 (MQ=255)
                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  2:3207077/93‑1 (MQ=255)
                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  <  2:3134660/93‑1 (MQ=255)
                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  >  2:3405680/1‑93 (MQ=255)
                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  <  2:3726341/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATA  >  NC_000913/4045099‑4045285
                                                                                             |                                                                                             
ACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGT                                                                                               <  SRR4302535.2274674/94‑1 (MQ=60)
ACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGT                                                                                               >  SRR4302535.2246225/1‑94 (MQ=60)
ACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGT                                                                                               >  SRR4302535.1508261/1‑94 (MQ=60)
 CTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCTGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTC                                                                                              <  SRR4302535.1319882/94‑1 (MQ=60)
 CTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTC                                                                                              <  SRR4302535.2125677/94‑1 (MQ=60)
 CTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTC                                                                                              >  SRR4302535.2814654/1‑94 (MQ=60)
 CTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTC                                                                                              >  SRR4302535.2742199/1‑94 (MQ=60)
   CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGA                                                                                            <  SRR4302535.2670518/94‑1 (MQ=60)
    CTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAG                                                                                           <  SRR4302535.928135/94‑1 (MQ=60)
    CTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAG                                                                                           <  SRR4302535.911331/94‑1 (MQ=60)
    CTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAG                                                                                           >  SRR4302535.2682275/1‑94 (MQ=60)
     TCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGAATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGC                                                                                          <  SRR4302535.4481651/94‑1 (MQ=60)
      CTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCC                                                                                         <  SRR4302535.564909/94‑1 (MQ=60)
      CTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCC                                                                                         >  SRR4302535.4660837/1‑94 (MQ=60)
       TGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCA                                                                                        >  SRR4302535.1445351/1‑94 (MQ=60)
       TGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCA                                                                                        >  SRR4302535.1178574/1‑94 (MQ=60)
       TGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCA                                                                                        >  SRR4302535.2346569/1‑94 (MQ=60)
        GATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAG                                                                                       <  SRR4302535.1304456/94‑1 (MQ=60)
         ATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGA                                                                                      <  SRR4302535.3617978/94‑1 (MQ=60)
            ACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAA                                                                                   >  SRR4302535.3759733/1‑94 (MQ=60)
             CATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAA                                                                                  >  SRR4302535.1411993/1‑94 (MQ=60)
             CATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAA                                                                                  >  SRR4302535.458022/1‑94 (MQ=60)
              ATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAA                                                                                 >  SRR4302535.4324223/1‑94 (MQ=60)
              ATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAA                                                                                 >  SRR4302535.3264057/1‑94 (MQ=60)
               TTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAAT                                                                                <  SRR4302535.1559794/94‑1 (MQ=60)
                     GCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGG                                                                          <  SRR4302535.521219/94‑1 (MQ=60)
                     GCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGG                                                                          <  SRR4302535.2044050/94‑1 (MQ=60)
                     GCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGG                                                                          <  SRR4302535.1824855/94‑1 (MQ=60)
                      CGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGG                                                                         >  SRR4302535.2473598/1‑94 (MQ=60)
                        TACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTT                                                                       >  SRR4302535.2866888/1‑94 (MQ=60)
                         ACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTG                                                                      >  SRR4302535.1543028/1‑94 (MQ=60)
                           CGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAG                                                                    <  SRR4302535.3538213/94‑1 (MQ=60)
                           CGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAG                                                                    <  SRR4302535.2737291/94‑1 (MQ=60)
                             GGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCT                                                                  >  SRR4302535.1353556/1‑94 (MQ=60)
                              GCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTT                                                                 >  SRR4302535.3367249/1‑94 (MQ=60)
                              GCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTT                                                                 <  SRR4302535.2657232/94‑1 (MQ=60)
                              GCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTT                                                                 <  SRR4302535.2525682/94‑1 (MQ=60)
                               CGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTC                                                                >  SRR4302535.4115027/1‑94 (MQ=60)
                                GTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCC                                                               <  SRR4302535.1093874/94‑1 (MQ=60)
                                  CGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGT                                                             >  SRR4302535.2645532/1‑94 (MQ=60)
                                  CGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGT                                                             >  SRR4302535.799313/1‑94 (MQ=60)
                                    GTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGG                                                           >  SRR4302535.1733318/1‑94 (MQ=60)
                                    GTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGG                                                           <  SRR4302535.4704336/94‑1 (MQ=60)
                                    GTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGG                                                           <  SRR4302535.3548188/94‑1 (MQ=60)
                                     TGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGC                                                          >  SRR4302535.322884/1‑94 (MQ=60)
                                      GAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCG                                                         <  SRR4302535.3059647/94‑1 (MQ=60)
                                       AAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGC                                                        >  SRR4302535.109311/1‑94 (MQ=60)
                                         AAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGA                                                      >  SRR4302535.4156680/1‑94 (MQ=60)
                                              CGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACA                                                 <  SRR4302535.281078/94‑1 (MQ=60)
                                                CGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAT                                               >  SRR4302535.3918677/1‑94 (MQ=60)
                                                CGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAT                                               <  SRR4302535.3604260/94‑1 (MQ=60)
                                                 GCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATG                                              >  SRR4302535.57053/1‑94 (MQ=60)
                                                 GCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATG                                              <  SRR4302535.3651590/94‑1 (MQ=60)
                                                  CAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGG                                             >  SRR4302535.4687882/1‑94 (MQ=60)
                                                   AGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGC                                            >  SRR4302535.1799324/1‑94 (MQ=60)
                                                   AGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGC                                            >  SRR4302535.2021086/1‑94 (MQ=60)
                                                    GGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCA                                           >  SRR4302535.1868930/1‑94 (MQ=60)
                                                      CATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGC                                         >  SRR4302535.1159177/1‑94 (MQ=60)
                                                       ATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCG                                        <  SRR4302535.2924225/94‑1 (MQ=60)
                                                       ATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCG                                        <  SRR4302535.2653939/94‑1 (MQ=60)
                                                           CTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGC                                    >  SRR4302535.4480542/1‑94 (MQ=60)
                                                            TGCAAGGTCTTGGCGGACTGGATACGCTTTACGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCT                                   <  SRR4302535.2015054/94‑1 (MQ=60)
                                                            TGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCT                                   >  SRR4302535.3625868/1‑94 (MQ=60)
                                                            TGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCT                                   >  SRR4302535.3625870/1‑94 (MQ=60)
                                                            TGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCT                                   <  SRR4302535.2212763/94‑1 (MQ=60)
                                                             GCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTC                                  >  SRR4302535.619355/1‑94 (MQ=60)
                                                             GCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTC                                  >  SRR4302535.337587/1‑94 (MQ=60)
                                                              CAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCG                                 >  SRR4302535.2558125/1‑94 (MQ=60)
                                                              CAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCG                                 >  SRR4302535.2416606/1‑94 (MQ=60)
                                                               AAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGA                                >  SRR4302535.4424967/1‑94 (MQ=60)
                                                               AAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGA                                >  SRR4302535.304922/1‑94 (MQ=60)
                                                               AAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGA                                >  SRR4302535.2911884/1‑94 (MQ=60)
                                                                AGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAG                               <  SRR4302535.384137/94‑1 (MQ=60)
                                                                 GGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGC                              >  SRR4302535.2476754/1‑94 (MQ=60)
                                                                  GTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCA                             >  SRR4302535.4174475/1‑94 (MQ=60)
                                                                   TCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAA                            <  SRR4302535.2721649/94‑1 (MQ=60)
                                                                      TGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAC                         <  SRR4302535.4188770/94‑1 (MQ=60)
                                                                      TGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAC                         >  SRR4302535.3210441/1‑94 (MQ=60)
                                                                      TGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAC                         <  SRR4302535.1518942/94‑1 (MQ=60)
                                                                      TGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAC                         <  SRR4302535.2495611/94‑1 (MQ=60)
                                                                       GGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGTAGCTCGAGCAAAACA                        >  SRR4302535.4124922/1‑94 (MQ=60)
                                                                       GGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACA                        <  SRR4302535.3411032/94‑1 (MQ=60)
                                                                         CGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAA                      >  SRR4302535.4023457/1‑94 (MQ=60)
                                                                         CGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAA                      >  SRR4302535.4592468/1‑94 (MQ=60)
                                                                          GGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAG                     <  SRR4302535.4563486/94‑1 (MQ=60)
                                                                          GGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAG                     >  SRR4302535.4690644/1‑94 (MQ=60)
                                                                           GACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGA                    <  SRR4302535.2831751/94‑1 (MQ=60)
                                                                            ACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAA                   >  SRR4302535.3757799/1‑94 (MQ=60)
                                                                            ACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAA                   <  SRR4302535.4447333/94‑1 (MQ=60)
                                                                             CTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAG                  <  SRR4302535.2369690/94‑1 (MQ=60)
                                                                             CTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAG                  >  SRR4302535.1682036/1‑94 (MQ=60)
                                                                             CTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAG                  <  SRR4302535.624393/94‑1 (MQ=60)
                                                                             CTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAG                  <  SRR4302535.10455/94‑1 (MQ=60)
                                                                              TGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGT                 >  SRR4302535.1417945/1‑94 (MQ=60)
                                                                              TGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGT                 >  SRR4302535.4624605/1‑94 (MQ=60)
                                                                               GGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTT                <  SRR4302535.107212/94‑1 (MQ=60)
                                                                               GGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTT                <  SRR4302535.3736717/94‑1 (MQ=60)
                                                                               GGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTT                <  SRR4302535.1458310/94‑1 (MQ=60)
                                                                                GATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTG               <  SRR4302535.1547133/94‑1 (MQ=60)
                                                                                 ATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGC              >  SRR4302535.2038200/1‑94 (MQ=60)
                                                                                  TACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCT             <  SRR4302535.308251/94‑1 (MQ=60)
                                                                                   ACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTT            <  SRR4302535.4011301/94‑1 (MQ=60)
                                                                                   ACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTT            <  SRR4302535.2503865/94‑1 (MQ=60)
                                                                                     GCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAT          >  SRR4302535.3235333/1‑94 (MQ=60)
                                                                                     GCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAT          >  SRR4302535.3149540/1‑94 (MQ=60)
                                                                                      CTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATC         <  SRR4302535.2107461/94‑1 (MQ=60)
                                                                                      CTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATC         >  SRR4302535.3749021/1‑94 (MQ=60)
                                                                                        GTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTC       <  SRR4302535.3945885/94‑1 (MQ=60)
                                                                                         TATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT      <  SRR4302535.196420/94‑1 (MQ=60)
                                                                                         TATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT      <  SRR4302535.3098437/94‑1 (MQ=60)
                                                                                         TATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT      >  SRR4302535.2312233/1‑94 (MQ=60)
                                                                                         TATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT      <  SRR4302535.1185355/94‑1 (MQ=60)
                                                                                           TGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCA    >  SRR4302535.4264687/1‑94 (MQ=60)
                                                                                           TGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCACCGAAGCTCGAGCAAAACAAAGAAGTTTCTTATCTCTCA    >  SRR4302535.991224/1‑94 (MQ=60)
                                                                                            GTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCAT   >  SRR4302535.548237/1‑94 (MQ=60)
                                                                                            GTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCAT   <  SRR4302535.4662929/94‑1 (MQ=60)
                                                                                            GTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCAT   <  SRR4302535.1231894/94‑1 (MQ=60)
                                                                                            GTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCAT   <  SRR4302535.4377566/94‑1 (MQ=60)
                                                                                             TCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATA  <  SRR4302535.4614705/94‑1 (MQ=60)
                                                                                             TCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGAAGCGAAGCTCGAGCAAAACAAAGAAGATGATTATCTCTCATA  >  SRR4302535.3119013/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACTCCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCTCATA  >  NC_000913/4045099‑4045285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑