Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,052,844	T→C	I5M (ATA→ATG)	glnG ←	GlnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,052,844	0	T	C	99.2%	799.9 / ‑3.3	258	I5M (ATA→ATG)	glnG	GlnG
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (127/129); total (127/131)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 264 total aligned reads displayed.

GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933

 ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATc                                                                                        >  1:3788958/1‑93 (MQ=255)

  tctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATcc                                                                                       >  1:2133414/1‑93 (MQ=255)

  tctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATcc                                                                                       >  2:4335436/1‑93 (MQ=255)

  tctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATcc                                                                                       >  2:3861292/1‑93 (MQ=255)

   ctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATccc                                                                                      <  1:4106692/93‑1 (MQ=255)

   ctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATccc                                                                                      <  2:1461275/93‑1 (MQ=255)

    tcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATcccc                                                                                     >  1:1086891/1‑92 (MQ=255)

    tcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCt                                                                                     >  1:395786/1‑93 (MQ=255)

    tcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCt                                                                                     >  1:2879960/1‑93 (MQ=255)

       aaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCTCTCGt                                                                                  >  1:4595554/1‑93 (MQ=255)

       aaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGt                                                                                  >  1:3478264/1‑93 (MQ=255)

       aaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGt                                                                                  >  1:1129841/1‑93 (MQ=255)

         cgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTg                                                                                <  2:3374074/93‑1 (MQ=255)

         cgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTg                                                                                >  2:2653308/1‑93 (MQ=255)

            cgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCAt                                                                             <  2:1080773/93‑1 (MQ=255)

             gtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATa                                                                            >  1:1298645/1‑93 (MQ=255)

               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          <  1:195165/93‑1 (MQ=255)

               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          >  2:1010578/1‑93 (MQ=255)

               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          >  1:2081147/1‑93 (MQ=255)

                cAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAc                                                                         <  2:1332708/93‑1 (MQ=255)

                 aGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACg                                                                        <  2:3829397/93‑1 (MQ=255)

                 aGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACg                                                                        <  1:3316523/93‑1 (MQ=255)

                  ggTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGt                                                                       >  1:1555145/1‑93 (MQ=255)

                  ggTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGt                                                                       >  2:4108029/1‑93 (MQ=255)

                    ttAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCa                                                                     >  2:1166971/1‑93 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  >  1:467741/1‑93 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  <  1:3861756/93‑1 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  >  2:4294643/1‑93 (MQ=255)

                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCt                                                                  >  1:1163479/1‑93 (MQ=255)

                         ccTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCttt                                                                <  1:3010083/93‑1 (MQ=255)

                           tGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTACCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAt                                                              <  1:2684316/93‑1 (MQ=255)

                            gCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAtt                                                             >  1:3171490/1‑93 (MQ=255)

                            gCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAtt                                                             >  2:3006515/1‑93 (MQ=255)

                                  gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGa                                                       >  2:2252943/1‑93 (MQ=255)

                                  gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGa                                                       >  1:508775/1‑93 (MQ=255)

                                    gagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATa                                                     <  1:717545/93‑1 (MQ=255)

                                     agcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                    <  2:212917/93‑1 (MQ=255)

                                     agcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                    <  2:267637/93‑1 (MQ=255)

                                     agcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAg                                                    <  1:1354554/93‑1 (MQ=255)

                                      gcgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAgg                                                   <  1:4117856/93‑1 (MQ=255)

                                       cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGc                                                  <  1:247215/93‑1 (MQ=255)

                                        gcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCa                                                 <  1:693145/93‑1 (MQ=255)

                                         cACGTTCAAGCACCCAACGGATGGGACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAg                                                <  2:2366466/93‑1 (MQ=255)

                                          aCGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                               <  2:4415222/93‑1 (MQ=255)

                                            gTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                             <  1:1020439/93‑1 (MQ=255)

                                             ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTaa                                            >  1:3066813/1‑93 (MQ=255)

                                             ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTaa                                            >  2:319502/1‑93 (MQ=255)

                                             ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCACCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTaa                                            <  2:3581661/93‑1 (MQ=255)

                                              tCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTaaa                                           <  2:4647915/93‑1 (MQ=255)

                                              tCAAGCAACCAACGGATGGAACTATCGTCATCGACTACCCAGACCATACCTCGCTGCATCAACGTCACCTTTATTTCCTGATAGGCAGGTaaa                                           >  1:1511013/1‑93 (MQ=255)

                                               cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                          <  1:163804/93‑1 (MQ=255)

                                                caGCCCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                         <  1:2251776/92‑1 (MQ=255)

                                                aaGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                         <  1:3664787/93‑1 (MQ=255)

                                                 aGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAACg                                        >  1:2749243/1‑93 (MQ=255)

                                                   cACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCgag                                      <  1:2367558/93‑1 (MQ=255)

                                                     cccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                    >  2:2961909/1‑93 (MQ=255)

                                                     cccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                    >  1:1660126/1‑93 (MQ=255)

                                                     cccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                    <  1:3834850/93‑1 (MQ=255)

                                                      ccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAc                                   <  1:3585984/93‑1 (MQ=255)

                                                       cAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACt                                  >  2:3442286/1‑93 (MQ=255)

                                                        aaCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTc                                 <  1:3919569/93‑1 (MQ=255)

                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             <  2:3889496/93‑1 (MQ=255)

                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             >  2:3287722/1‑93 (MQ=255)

                                                              tGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATg                           >  1:4222086/1‑93 (MQ=255)

                                                              tGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATg                           >  2:185106/1‑93 (MQ=255)

                                                                 atcTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  2:541695/91‑1 (MQ=255)

                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  2:4463463/93‑1 (MQ=255)

                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  2:832248/93‑1 (MQ=255)

                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  1:4046518/93‑1 (MQ=255)

                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  1:4384975/93‑1 (MQ=255)

                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       >  2:2288002/1‑93 (MQ=255)

                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       <  2:1583456/93‑1 (MQ=255)

                                                                   cTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTg                      <  1:373245/93‑1 (MQ=255)

                                                                   cTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTg                      <  1:1852887/93‑1 (MQ=255)

                                                                    tATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTgg                     >  2:419580/1‑93 (MQ=255)

                                                                     aTCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGc                    <  2:3121925/93‑1 (MQ=255)

                                                                      tCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGcc                   >  1:4514651/1‑93 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                >  1:3396021/1‑93 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                >  1:3660656/1‑93 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                <  2:1826845/93‑1 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                <  1:262080/93‑1 (MQ=255)

                                                                         tCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAc                >  1:1531864/1‑93 (MQ=255)

                                                                          cATCGACTACCCAGACCATCCCTCGATGCATAATCGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACt               <  1:3127993/93‑1 (MQ=255)

                                                                           aTCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTg              >  2:2010775/1‑93 (MQ=255)

                                                                             cGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGt            <  2:2799241/93‑1 (MQ=255)

                                                                              gACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTa           >  2:2477106/1‑93 (MQ=255)

                                                                                cTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa         <  1:4270853/93‑1 (MQ=255)

                                                                                cTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa         >  2:4001086/1‑93 (MQ=255)

                                                                                cTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaaa         <  1:1407900/93‑1 (MQ=255)

                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        >  1:2541481/1‑93 (MQ=255)

                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        <  2:1678732/93‑1 (MQ=255)

                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        >  2:2922937/1‑93 (MQ=255)

                                                                                  aCCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt       <  2:233390/93‑1 (MQ=255)

                                                                                   cccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc      <  1:1808288/93‑1 (MQ=255)

                                                                                   cccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc      <  2:1398640/93‑1 (MQ=255)

                                                                                   cccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc      >  2:3501244/1‑93 (MQ=255)

                                                                                     cAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCaa    <  2:3689040/93‑1 (MQ=255)

                                                                                     cAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTCGTAAATTCaa    >  1:852680/1‑93 (MQ=255)

                                                                                      aGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt   <  1:3645152/93‑1 (MQ=255)

                                                                                       gACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt  >  2:611416/1‑93 (MQ=255)

GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  NC_000913/4052751‑4052937

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACC                                                                                               >  SRR4302535.2997596/1‑94 (MQ=60)

CCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACC                                                                                               <  SRR4302535.1400329/94‑1 (MQ=60)

  CGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAT                                                                                             >  SRR4302535.3253070/1‑94 (MQ=60)

  CGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAT                                                                                             <  SRR4302535.1807125/94‑1 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                            >  SRR4302535.3815091/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                            >  SRR4302535.271424/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                            >  SRR4302535.4172218/1‑94 (MQ=60)

   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                            >  SRR4302535.1323405/1‑94 (MQ=60)

    TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCC                                                                                           >  SRR4302535.2148790/1‑94 (MQ=60)

     TCTCAAGCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                          <  SRR4302535.1827522/94‑1 (MQ=60)

     TCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                          <  SRR4302535.2817222/94‑1 (MQ=60)

     TCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                          >  SRR4302535.140425/1‑94 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                         <  SRR4302535.4134811/94‑1 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                         >  SRR4302535.2900350/1‑94 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                         >  SRR4302535.398557/1‑94 (MQ=60)

      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCC                                                                                         >  SRR4302535.1094618/1‑94 (MQ=60)

       TCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTC                                                                                        <  SRR4302535.3736562/94‑1 (MQ=60)

        CAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCG                                                                                       >  SRR4302535.2334090/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCTCTCGT                                                                                      >  SRR4302535.4626822/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                      >  SRR4302535.3502433/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                      >  SRR4302535.433642/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                      >  SRR4302535.2586952/1‑94 (MQ=60)

         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                      >  SRR4302535.1137907/1‑94 (MQ=60)

          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTT                                                                                     <  SRR4302535.2776330/94‑1 (MQ=60)

              TCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCAT                                                                                 >  SRR4302535.3837584/1‑94 (MQ=60)

              TCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCAT                                                                                 <  SRR4302535.428590/94‑1 (MQ=60)

               CGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATA                                                                                >  SRR4302535.1307906/1‑94 (MQ=60)

                GTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAA                                                                               >  SRR4302535.2053710/1‑94 (MQ=60)

                GTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAA                                                                               >  SRR4302535.3096409/1‑94 (MQ=60)

                 TACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAA                                                                              >  SRR4302535.3943710/1‑94 (MQ=60)

                 TACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAA                                                                              >  SRR4302535.2096140/1‑94 (MQ=60)

                  ACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAC                                                                             <  SRR4302535.196507/94‑1 (MQ=60)

                   CAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACG                                                                            <  SRR4302535.1537971/94‑1 (MQ=60)

                    AGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGT                                                                           >  SRR4302535.1566353/1‑94 (MQ=60)

                    AGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGT                                                                           <  SRR4302535.3339598/94‑1 (MQ=60)

                     GGTTGAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTC                                                                          >  SRR4302535.1662929/1‑94 (MQ=60)

                     GGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTC                                                                          >  SRR4302535.1848753/1‑94 (MQ=60)

                     GGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTC                                                                          <  SRR4302535.2572631/94‑1 (MQ=60)

                     GGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTC                                                                          >  SRR4302535.709344/1‑94 (MQ=60)

                      GTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCA                                                                         <  SRR4302535.1112272/94‑1 (MQ=60)

                      GTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCA                                                                         >  SRR4302535.540650/1‑94 (MQ=60)

                       TTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCAC                                                                        >  SRR4302535.318197/1‑94 (MQ=60)

                        TAAACCTGCGCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACC                                                                       <  SRR4302535.2318293/94‑1 (MQ=60)

                        TAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACC                                                                       >  SRR4302535.1911936/1‑94 (MQ=60)

                        TAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACC                                                                       <  SRR4302535.1301015/94‑1 (MQ=60)

                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                      >  SRR4302535.471013/1‑94 (MQ=60)

                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                      >  SRR4302535.1171789/1‑94 (MQ=60)

                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                      <  SRR4302535.745626/94‑1 (MQ=60)

                          AACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                     <  SRR4302535.3888328/94‑1 (MQ=60)

                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                    >  SRR4302535.2463123/1‑94 (MQ=60)

                            CCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTA                                                                   <  SRR4302535.3031167/94‑1 (MQ=60)

                              TGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                                 >  SRR4302535.3193448/1‑94 (MQ=60)

                              TGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                                 <  SRR4302535.3953439/94‑1 (MQ=60)

                              TGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTACCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                                 <  SRR4302535.2703511/94‑1 (MQ=60)

                               GCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTT                                                                >  SRR4302535.2440490/1‑94 (MQ=60)

                                 CCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCC                                                              <  SRR4302535.113815/94‑1 (MQ=60)

                                  CCAGCGAGCGCACGTTCAAGCACCCAGCGGATGGACCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCGCCTTTATTTCCT                                                             <  SRR4302535.3560364/94‑1 (MQ=60)

                                   CAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTG                                                            <  SRR4302535.1579157/94‑1 (MQ=60)

                                    AGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGA                                                           >  SRR4302535.512332/1‑94 (MQ=60)

                                    AGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGA                                                           >  SRR4302535.3206245/1‑94 (MQ=60)

                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                        <  SRR4302535.722585/94‑1 (MQ=60)

                                        AGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGG                                                       <  SRR4302535.1364242/94‑1 (MQ=60)

                                         GCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGC                                                      <  SRR4302535.4146040/94‑1 (MQ=60)

                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                     <  SRR4302535.248909/94‑1 (MQ=60)

                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGCCACCTTTATTTCCTGATAGGCA                                                     <  SRR4302535.770740/94‑1 (MQ=60)

                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                    <  SRR4302535.3904570/94‑1 (MQ=60)

                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                    <  SRR4302535.698004/94‑1 (MQ=60)

                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                    <  SRR4302535.3979001/94‑1 (MQ=60)

                                            CACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGG                                                   >  SRR4302535.1445798/1‑94 (MQ=60)

                                            CACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGG                                                   >  SRR4302535.3518497/1‑94 (MQ=60)

                                            CACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGG                                                   <  SRR4302535.1753201/94‑1 (MQ=60)

                                              CGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTA                                                 >  SRR4302535.3820238/1‑94 (MQ=60)

                                               GTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAA                                                >  SRR4302535.3088242/1‑94 (MQ=60)

                                               GTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAA                                                <  SRR4302535.1027691/94‑1 (MQ=60)

                                               GTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAA                                                <  SRR4302535.210717/94‑1 (MQ=60)

                                               GTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAA                                                >  SRR4302535.3490193/1‑94 (MQ=60)

                                                TTCAAGCAACCAACGGATGGAACTATCGTCATCGACTACCCAGACCATACCTCGCTGCATCAACGTCACCTTTATTTCCTGATAGGCAGGTAAA                                               >  SRR4302535.1521898/1‑94 (MQ=60)

                                                 TCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAC                                              <  SRR4302535.486634/94‑1 (MQ=60)

                                                 TCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAC                                              <  SRR4302535.3685656/94‑1 (MQ=60)

                                                 TCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAC                                              <  SRR4302535.3357218/94‑1 (MQ=60)

                                                  CAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACC                                             <  SRR4302535.164948/94‑1 (MQ=60)

                                                   cagccCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCG                                            <  SRR4302535.2267811/89‑1 (MQ=60)

                                                   AAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCG                                            <  SRR4302535.3690165/94‑1 (MQ=60)

                                                   AAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAACG                                            >  SRR4302535.2768842/1‑94 (MQ=60)

                                                    AGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGA                                           >  SRR4302535.2145685/1‑94 (MQ=60)

                                                     GCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAG                                          <  SRR4302535.3687455/94‑1 (MQ=60)

                                                      CACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGA                                         <  SRR4302535.2384556/94‑1 (MQ=60)

                                                      CACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGA                                         >  SRR4302535.166302/1‑94 (MQ=60)

                                                       CCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAA                                        <  SRR4302535.2241551/94‑1 (MQ=60)

                                                       ACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAA                                        >  SRR4302535.1672124/1‑94 (MQ=60)

                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       <  SRR4302535.3875/94‑1 (MQ=60)

                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       <  SRR4302535.3861295/94‑1 (MQ=60)

                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                      <  SRR4302535.118263/94‑1 (MQ=60)

                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                      <  SRR4302535.3610859/94‑1 (MQ=60)

                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                      >  SRR4302535.1648124/1‑94 (MQ=60)

                                                          CAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTC                                     >  SRR4302535.4424775/1‑94 (MQ=60)

                                                           CACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCG                                    <  SRR4302535.1103709/94‑1 (MQ=60)

                                                           AACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCG                                    <  SRR4302535.3946374/94‑1 (MQ=60)

                                                           AACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCG                                    >  SRR4302535.4611142/1‑94 (MQ=60)

                                                             CGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGT                                  >  SRR4302535.3794224/1‑94 (MQ=60)

                                                              GGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTA                                 >  SRR4302535.4113313/1‑94 (MQ=60)

                                                              GGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTA                                 >  SRR4302535.2918866/1‑94 (MQ=60)

                                                              GGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGCA                                 >  SRR4302535.3077939/1‑94 (MQ=60)

                                                               GATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTAT                                >  SRR4302535.2904813/1‑94 (MQ=60)

                                                                ATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATG                               >  SRR4302535.4250976/1‑94 (MQ=60)

                                                                ATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATG                               >  SRR4302535.1057538/1‑94 (MQ=60)

                                                                ATGGAACCATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATG                               <  SRR4302535.4122174/94‑1 (MQ=60)

                                                                  GGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCC                             <  SRR4302535.4638867/94‑1 (MQ=60)

                                                                   GAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCC                            <  SRR4302535.1540259/94‑1 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                           >  SRR4302535.3099126/1‑94 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                           <  SRR4302535.4414976/94‑1 (MQ=60)

                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                           <  SRR4302535.4074217/94‑1 (MQ=60)

                                                                     ACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGCATGCCCTG                          >  SRR4302535.933799/1‑94 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         <  SRR4302535.3736144/94‑1 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         <  SRR4302535.2669635/94‑1 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         <  SRR4302535.2679750/94‑1 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         >  SRR4302535.4034274/1‑94 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         <  SRR4302535.1866234/94‑1 (MQ=60)

                                                                      CTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGG                         <  SRR4302535.375829/94‑1 (MQ=60)

                                                                        ATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCC                       >  SRR4302535.4545607/1‑94 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    >  SRR4302535.3419647/1‑94 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    >  SRR4302535.1542900/1‑94 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    >  SRR4302535.2496313/1‑94 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    <  SRR4302535.3617151/94‑1 (MQ=60)

                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    >  SRR4302535.3685993/1‑94 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                   <  SRR4302535.263900/94‑1 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                   <  SRR4302535.944958/94‑1 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                   <  SRR4302535.4329571/94‑1 (MQ=60)

                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATGTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                   <  SRR4302535.413780/94‑1 (MQ=60)

                                                                             CATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG                  <  SRR4302535.1514761/94‑1 (MQ=60)

                                                                             CATCGACTACCCAGACCATCCCTCGATGCATAATCGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG                  <  SRR4302535.3149777/94‑1 (MQ=60)

                                                                              ATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGG                 >  SRR4302535.4144434/1‑94 (MQ=60)

                                                                              ATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGG                 <  SRR4302535.155282/94‑1 (MQ=60)

                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT                <  SRR4302535.199231/94‑1 (MQ=60)

                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA               >  SRR4302535.4297290/1‑94 (MQ=60)

                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA               <  SRR4302535.3688704/94‑1 (MQ=60)

                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA               <  SRR4302535.4673876/94‑1 (MQ=60)

                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              >  SRR4302535.4573027/1‑94 (MQ=60)

                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            <  SRR4302535.4300090/94‑1 (MQ=60)

                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            <  SRR4302535.1417958/94‑1 (MQ=60)

                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            >  SRR4302535.2559786/1‑94 (MQ=60)

                                                                                     ACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTC          >  SRR4302535.3611608/1‑94 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA         <  SRR4302535.1821376/94‑1 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA         <  SRR4302535.591303/94‑1 (MQ=60)

                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA         <  SRR4302535.1319911/94‑1 (MQ=60)

                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAA        >  SRR4302535.3378149/1‑94 (MQ=60)

                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTCGTAAATTCAA        >  SRR4302535.858686/1‑94 (MQ=60)

                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT      <  SRR4302535.3670396/94‑1 (MQ=60)

                                                                                           ACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTT    <  SRR4302535.3529529/94‑1 (MQ=60)

                                                                                           ACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTT    <  SRR4302535.110798/94‑1 (MQ=60)

                                                                                            CCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTG   >  SRR4302535.637293/1‑94 (MQ=60)

                                                                                            CCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTG   <  SRR4302535.2727153/94‑1 (MQ=60)

                                                                                             CATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  SRR4302535.509571/1‑94 (MQ=60)

                                                                                             CATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  SRR4302535.3644494/1‑94 (MQ=60)

GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  NC_000913/4052751‑4052937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`