Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,532	A→G	R957R (CGT→CGC)	fdoG ←	formate dehydrogenase‑O, major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,532	0	A	G	99.6%	817.9 / ‑4.9	257	R957R (CGT→CGC)	fdoG	formate dehydrogenase‑O, major subunit
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (145/111); total (145/112)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 266 total aligned reads displayed.

AAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGC  >  NC_000913/4080442‑4080621

 aaGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGt                                                                                        <  1:516066/93‑1 (MQ=255)

  aGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGtt                                                                                       <  1:2196388/93‑1 (MQ=255)

   gCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGttt                                                                                      >  1:1359824/1‑93 (MQ=255)

   gCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGttt                                                                                      >  2:1468358/1‑93 (MQ=255)

   gCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGttt                                                                                      >  2:1311917/1‑93 (MQ=255)

    ccTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTg                                                                                     >  1:3840170/1‑93 (MQ=255)

    ccTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTg                                                                                     >  2:525542/1‑93 (MQ=255)

     cTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTgg                                                                                    >  2:1076920/1‑93 (MQ=255)

     cTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTgg                                                                                    >  1:1887380/1‑93 (MQ=255)

       tttttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTc                                                                                  >  1:2609894/1‑93 (MQ=255)

       tttttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTc                                                                                  <  1:3864072/93‑1 (MQ=255)

        ttttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTcc                                                                                 >  1:478030/1‑92 (MQ=255)

        ttttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTca                                                                                 >  1:14248/1‑93 (MQ=255)

        ttttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGGTTGGTca                                                                                 >  2:4270160/1‑93 (MQ=255)

         tttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTcac                                                                                >  1:4125453/1‑93 (MQ=255)

         tttAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTcac                                                                                >  1:2978768/1‑93 (MQ=255)

            aGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTcaccac                                                                             >  1:3236807/1‑93 (MQ=255)

             gCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCaccacc                                                                            >  2:3489482/1‑93 (MQ=255)

              cTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCacaaccc                                                                           >  1:226741/1‑92 (MQ=255)

              cTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCg                                                                           >  1:4325751/1‑93 (MQ=255)

               tACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgc                                                                          >  1:4301170/1‑93 (MQ=255)

               tACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgc                                                                          >  1:2846110/1‑93 (MQ=255)

                 cGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctt                                                                        <  2:1102093/93‑1 (MQ=255)

                   ccTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttg                                                                      >  1:309702/1‑93 (MQ=255)

                   ccTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttg                                                                      <  1:3476004/93‑1 (MQ=255)

                    cTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttgg                                                                     <  1:2456833/93‑1 (MQ=255)

                    cTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttgg                                                                     >  2:323983/1‑93 (MQ=255)

                    cTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttgg                                                                     >  2:2280636/1‑93 (MQ=255)

                     ttCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggc                                                                    <  1:4579794/93‑1 (MQ=255)

                     ttCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggc                                                                    >  2:2636722/1‑93 (MQ=255)

                       cATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggctt                                                                  <  1:861646/93‑1 (MQ=255)

                        aTAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggcttt                                                                 >  1:1659345/1‑93 (MQ=255)

                        aTAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggcttt                                                                 <  1:1507772/93‑1 (MQ=255)

                        aTAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggcttt                                                                 <  1:941179/93‑1 (MQ=255)

                        aTAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCCCTTTCAGCGTGCGAATGCGTTTGGTCACCACCgctttggcttt                                                                 <  2:747465/93‑1 (MQ=255)

                            ccccAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGata                                                             <  1:744515/93‑1 (MQ=255)

                             cccAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGatat                                                            >  1:1754894/1‑93 (MQ=255)

                             cccAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGatat                                                            <  2:4487131/93‑1 (MQ=255)

                             cccAGTGAATCGGAATACCGATGGGATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGatat                                                            >  2:1649084/1‑93 (MQ=255)

                              ccAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGAtata                                                           <  2:1380111/93‑1 (MQ=255)

                              ccAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGAtata                                                           >  2:3186239/1‑93 (MQ=255)

                               cAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAg                                                          >  1:599819/1‑93 (MQ=255)

                                aGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGc                                                         >  2:562501/1‑93 (MQ=255)

                                  tGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCa                                                       <  1:3696431/93‑1 (MQ=255)

                                  tGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCa                                                       <  2:2853248/93‑1 (MQ=255)

                                  tGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCa                                                       >  2:701490/1‑93 (MQ=255)

                                   gAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCAc                                                      >  2:2535612/1‑93 (MQ=255)

                                   gAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCAc                                                      <  1:1328598/93‑1 (MQ=255)

                                      tCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGt                                                   <  2:2776457/93‑1 (MQ=255)

                                      tCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGt                                                   <  2:4321937/93‑1 (MQ=255)

                                       cGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGtt                                                  <  2:1851561/93‑1 (MQ=255)

                                         gAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTgg                                                <  2:3221019/93‑1 (MQ=255)

                                         gAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTgg                                                <  2:80091/93‑1 (MQ=255)

                                          aaTACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTgga                                               >  2:890663/1‑93 (MQ=255)

                                           aTACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTggag                                              <  1:107554/93‑1 (MQ=255)

                                           aTACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTggag                                              >  2:211100/1‑93 (MQ=255)

                                            tACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTggagg                                             >  1:3445612/1‑93 (MQ=255)

                                             aCCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTggagga                                            <  2:2197051/93‑1 (MQ=255)

                                              ccGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGgaggag                                           <  2:301386/93‑1 (MQ=255)

                                                 aTGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACt                                        <  2:4686155/93‑1 (MQ=255)

                                                 aTGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACt                                        <  2:3653078/93‑1 (MQ=255)

                                                  tGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACtt                                       <  1:4238000/93‑1 (MQ=255)

                                                  tGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACtt                                       >  2:4184982/1‑93 (MQ=255)

                                                   ggTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACttt                                      >  2:1825685/1‑93 (MQ=255)

                                                    gTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGAGTTTc                                     >  2:2226682/1‑93 (MQ=255)

                                                       tCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGGGTTTGGTCACGACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACg                                  >  2:385834/1‑93 (MQ=255)

                                                         gATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGt                                >  1:2933996/1‑93 (MQ=255)

                                                          atatCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTa                               >  1:4009298/1‑93 (MQ=255)

                                                          atatCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTa                               >  1:4384591/1‑93 (MQ=255)

                                                           tatCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAt                              >  2:4633251/1‑93 (MQ=255)

                                                           tatCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAt                              >  1:1013050/1‑93 (MQ=255)

                                                            atCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATc                             <  2:3367781/93‑1 (MQ=255)

                                                            atCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATc                             >  1:632577/1‑93 (MQ=255)

                                                             tCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCg                            <  2:50816/93‑1 (MQ=255)

                                                             tCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCAAGGTATCg                            >  2:739639/1‑93 (MQ=255)

                                                              cTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGc                           <  2:3122747/93‑1 (MQ=255)

                                                              cTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACAACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGc                           >  1:1616314/1‑93 (MQ=255)

                                                               tttGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGcc                          >  2:3674449/1‑93 (MQ=255)

                                                                   ccGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTgg                      <  2:3571091/93‑1 (MQ=255)

                                                                    cGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTggg                     <  1:4650510/93‑1 (MQ=255)

                                                                    cGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTggg                     >  1:1985786/1‑93 (MQ=255)

                                                                    cGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTggg                     <  1:188416/93‑1 (MQ=255)

                                                                      ttCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCa                   >  2:191104/1‑93 (MQ=255)

                                                                      ttCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCCGGGCa                   >  1:1251933/1‑93 (MQ=255)

                                                                        cGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAAt                 <  1:3557102/93‑1 (MQ=255)

                                                                        cGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAAt                 <  2:200839/93‑1 (MQ=255)

                                                                        cGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAAt                 <  1:319133/93‑1 (MQ=255)

                                                                          cTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGc               <  1:3306395/93‑1 (MQ=255)

                                                                           tttCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGcc              <  2:2734126/93‑1 (MQ=255)

                                                                           tttCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGcc              >  1:3948761/1‑93 (MQ=255)

                                                                            ttCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCa             <  2:1287806/93‑1 (MQ=255)

                                                                             tCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCaa            <  2:2822590/93‑1 (MQ=255)

                                                                                gCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGtt         >  1:1431744/1‑93 (MQ=255)

                                                                                gCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGtt         >  2:2970841/1‑93 (MQ=255)

                                                                                    gCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTAtt     <  2:4097819/93‑1 (MQ=255)

                                                                                    gCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTAtt     <  1:3813659/93‑1 (MQ=255)

                                                                                      gAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCg   >  1:3886400/1‑93 (MQ=255)

                                                                                      gAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCg   >  1:3144587/1‑93 (MQ=255)

                                                                                       aaTGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGTAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGc  <  1:2164247/93‑1 (MQ=255)

                                                                                       aaTGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGc  >  1:3584280/1‑93 (MQ=255)

AAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGC  >  NC_000913/4080442‑4080621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAGC  >  NC_000913/4080439‑4080625

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATG                                                                                               <  SRR4302535.548696/94‑1 (MQ=60)

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATG                                                                                               >  SRR4302535.2752342/1‑94 (MQ=60)

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATG                                                                                               <  SRR4302535.4212521/94‑1 (MQ=60)

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATG                                                                                               <  SRR4302535.3638656/94‑1 (MQ=60)

 ATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGC                                                                                              <  SRR4302535.3495777/94‑1 (MQ=60)

 ATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGC                                                                                              >  SRR4302535.4638124/1‑94 (MQ=60)

 ATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGC                                                                                              >  SRR4302535.1330241/1‑94 (MQ=60)

 ATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGC                                                                                              <  SRR4302535.2106023/94‑1 (MQ=60)

   AAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGGCGTTCGCTTTCAGCGTGCGAATACGT                                                                                            <  SRR4302535.4696074/94‑1 (MQ=60)

   AAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGT                                                                                            >  SRR4302535.840995/1‑94 (MQ=60)

    AAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTT                                                                                           <  SRR4302535.519672/94‑1 (MQ=60)

    AAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTT                                                                                           >  SRR4302535.387070/1‑94 (MQ=60)

     AGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTT                                                                                          >  SRR4302535.1369548/1‑94 (MQ=60)

     AGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTT                                                                                          <  SRR4302535.2212044/94‑1 (MQ=60)

     AGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTT                                                                                          >  SRR4302535.1116082/1‑94 (MQ=60)

     AGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTT                                                                                          <  SRR4302535.282278/94‑1 (MQ=60)

      GCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGTTTTCAGCGTGCGAATGCGTTTG                                                                                         <  SRR4302535.4680591/94‑1 (MQ=60)

      GCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTG                                                                                         >  SRR4302535.3866634/1‑94 (MQ=60)

      GCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTG                                                                                         <  SRR4302535.6483/94‑1 (MQ=60)

       CCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGG                                                                                        >  SRR4302535.1900976/1‑94 (MQ=60)

        CTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGT                                                                                       >  SRR4302535.4221790/1‑94 (MQ=60)

        CTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGT                                                                                       >  SRR4302535.4289015/1‑94 (MQ=60)

        CTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGT                                                                                       <  SRR4302535.565307/94‑1 (MQ=60)

         TTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTC                                                                                      >  SRR4302535.2628622/1‑94 (MQ=60)

          TTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCC                                                                                     >  SRR4302535.481381/1‑94 (MQ=60)

          TTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCA                                                                                     >  SRR4302535.14349/1‑94 (MQ=60)

          TTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCA                                                                                     <  SRR4302535.3890653/94‑1 (MQ=60)

           TTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCAC                                                                                    >  SRR4302535.2999668/1‑94 (MQ=60)

           TTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCAC                                                                                    >  SRR4302535.3200611/1‑94 (MQ=60)

           TTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCAC                                                                                    >  SRR4302535.4153687/1‑94 (MQ=60)

             TTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCA                                                                                  >  SRR4302535.1419716/1‑94 (MQ=60)

              TAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCAC                                                                                 >  SRR4302535.3259276/1‑94 (MQ=60)

                GCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACaaccc                                                                               >  SRR4302535.228302/1‑89 (MQ=60)

                GCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCG                                                                               >  SRR4302535.4355332/1‑94 (MQ=60)

                 CTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGC                                                                              >  SRR4302535.2866289/1‑94 (MQ=60)

                 CTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGC                                                                              >  SRR4302535.4330600/1‑94 (MQ=60)

                 CTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGC                                                                              >  SRR4302535.1397407/1‑94 (MQ=60)

                     GCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTG                                                                          >  SRR4302535.311851/1‑94 (MQ=60)

                     GCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTG                                                                          <  SRR4302535.4345510/94‑1 (MQ=60)

                     GCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTG                                                                          >  SRR4302535.3559161/1‑94 (MQ=60)

                     CCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTG                                                                          <  SRR4302535.1438013/94‑1 (MQ=60)

                      CCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGG                                                                         <  SRR4302535.3500161/94‑1 (MQ=60)

                       CTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGC                                                                        <  SRR4302535.2474550/94‑1 (MQ=60)

                        TTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCT                                                                       <  SRR4302535.1827572/94‑1 (MQ=60)

                        TTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCT                                                                       <  SRR4302535.4610988/94‑1 (MQ=60)

                          CATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTT                                                                     <  SRR4302535.867735/94‑1 (MQ=60)

                          CATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTT                                                                     >  SRR4302535.1671340/1‑94 (MQ=60)

                           ATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTG                                                                    <  SRR4302535.1518613/94‑1 (MQ=60)

                           ATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTG                                                                    <  SRR4302535.947842/94‑1 (MQ=60)

                            TAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGA                                                                   >  SRR4302535.2272088/1‑94 (MQ=60)

                              GCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATA                                                                 <  SRR4302535.1898461/94‑1 (MQ=60)

                              GCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATA                                                                 >  SRR4302535.2419272/1‑94 (MQ=60)

                               CCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATAT                                                                <  SRR4302535.749743/94‑1 (MQ=60)

                               CCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATAT                                                                >  SRR4302535.1767576/1‑94 (MQ=60)

                                 CCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAG                                                              >  SRR4302535.3293260/1‑94 (MQ=60)

                                 CCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAG                                                              <  SRR4302535.870910/94‑1 (MQ=60)

                                 CCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAG                                                              >  SRR4302535.604015/1‑94 (MQ=60)

                                  CAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGC                                                             <  SRR4302535.270301/94‑1 (MQ=60)

                                  CAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGC                                                             <  SRR4302535.1465655/94‑1 (MQ=60)

                                   AGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCC                                                            >  SRR4302535.325156/1‑94 (MQ=60)

                                   AGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCC                                                            >  SRR4302535.1979620/1‑94 (MQ=60)

                                     TGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCAC                                                          <  SRR4302535.3722012/94‑1 (MQ=60)

                                      GAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACG                                                         <  SRR4302535.1338077/94‑1 (MQ=60)

                                       AATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGG                                                        >  SRR4302535.680745/1‑94 (MQ=60)

                                          CGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTG                                                     >  SRR4302535.1613523/1‑94 (MQ=60)

                                           GGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGG                                                    <  SRR4302535.1367764/94‑1 (MQ=60)

                                           GGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGG                                                    <  SRR4302535.388530/94‑1 (MQ=60)

                                              ATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCATCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGG                                                 >  SRR4302535.4612742/1‑94 (MQ=60)

                                              ATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGG                                                 <  SRR4302535.108314/94‑1 (MQ=60)

                                              ATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGG                                                 >  SRR4302535.3131608/1‑94 (MQ=60)

                                              ATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGG                                                 >  SRR4302535.3469569/1‑94 (MQ=60)

                                               TACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGA                                                <  SRR4302535.2111181/94‑1 (MQ=60)

                                                TCCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAG                                               <  SRR4302535.3111771/94‑1 (MQ=60)

                                                  CTATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGAC                                             <  SRR4302535.504748/94‑1 (MQ=60)

                                                  CGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGAC                                             <  SRR4302535.3094885/94‑1 (MQ=60)

                                                    ATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTT                                           >  SRR4302535.3129887/1‑94 (MQ=60)

                                                    ATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTT                                           <  SRR4302535.2909517/94‑1 (MQ=60)

                                                    ATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTT                                           >  SRR4302535.3595969/1‑94 (MQ=60)

                                                     TGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTT                                          <  SRR4302535.4267001/94‑1 (MQ=60)

                                                      GGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTC                                         <  SRR4302535.4400143/94‑1 (MQ=60)

                                                      GGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTC                                         >  SRR4302535.3555978/1‑94 (MQ=60)

                                                      GGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTC                                         >  SRR4302535.3988474/1‑94 (MQ=60)

                                                        TATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCAC                                       >  SRR4302535.36211/1‑94 (MQ=60)

                                                          TCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGG                                     <  SRR4302535.222855/94‑1 (MQ=60)

                                                           CGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGT                                    >  SRR4302535.2954645/1‑94 (MQ=60)

                                                           CGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGT                                    >  SRR4302535.2417248/1‑94 (MQ=60)

                                                            GATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTA                                   >  SRR4302535.4606016/1‑94 (MQ=60)

                                                            GATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTA                                   >  SRR4302535.4036723/1‑94 (MQ=60)

                                                            GATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTA                                   <  SRR4302535.1368644/94‑1 (MQ=60)

                                                            GATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTA                                   >  SRR4302535.4414590/1‑94 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  >  SRR4302535.1718104/1‑94 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  >  SRR4302535.1020256/1‑94 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  >  SRR4302535.1217038/1‑94 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  >  SRR4302535.1542956/1‑94 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  <  SRR4302535.1807821/94‑1 (MQ=60)

                                                             ATATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTAT                                  >  SRR4302535.4354041/1‑94 (MQ=60)

                                                              TATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATC                                 >  SRR4302535.636984/1‑94 (MQ=60)

                                                              TATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATC                                 >  SRR4302535.1331717/1‑94 (MQ=60)

                                                              TATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATC                                 >  SRR4302535.3988873/1‑94 (MQ=60)

                                                               ATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGTTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCG                                <  SRR4302535.3912861/94‑1 (MQ=60)

                                                               ATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCG                                >  SRR4302535.1631228/1‑94 (MQ=60)

                                                               ATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCG                                >  SRR4302535.2078373/1‑94 (MQ=60)

                                                               ATCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCG                                >  SRR4302535.2905531/1‑94 (MQ=60)

                                                                TCTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACAACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGC                               >  SRR4302535.1627961/1‑94 (MQ=60)

                                                                 CTTTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCC                              <  SRR4302535.1685729/94‑1 (MQ=60)

                                                                   TTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCT                            <  SRR4302535.1050140/94‑1 (MQ=60)

                                                                   TTGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCT                            <  SRR4302535.2964824/94‑1 (MQ=60)

                                                                    TGCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTG                           >  SRR4302535.8131/1‑94 (MQ=60)

                                                                     GCCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGG                          <  SRR4302535.3783385/94‑1 (MQ=60)

                                                                      CCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGG                         >  SRR4302535.2000087/1‑94 (MQ=60)

                                                                      CCGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGG                         <  SRR4302535.3632837/94‑1 (MQ=60)

                                                                       CGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGC                        >  SRR4302535.1491438/1‑94 (MQ=60)

                                                                       CGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGC                        <  SRR4302535.189721/94‑1 (MQ=60)

                                                                       CGTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGC                        <  SRR4302535.4682002/94‑1 (MQ=60)

                                                                        GTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCA                       >  SRR4302535.3493770/1‑94 (MQ=60)

                                                                        GTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCA                       >  SRR4302535.2608714/1‑94 (MQ=60)

                                                                        GTTCGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCCGGGCA                       >  SRR4302535.1260872/1‑94 (MQ=60)

                                                                           CGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATG                    >  SRR4302535.1925996/1‑94 (MQ=60)

                                                                           CGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATG                    <  SRR4302535.3581775/94‑1 (MQ=60)

                                                                           CGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATG                    <  SRR4302535.484551/94‑1 (MQ=60)

                                                                           CGCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATG                    <  SRR4302535.321341/94‑1 (MQ=60)

                                                                            GCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGC                   <  SRR4302535.4298890/94‑1 (MQ=60)

                                                                            GCTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGC                   >  SRR4302535.3849211/1‑94 (MQ=60)

                                                                             CTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCC                  <  SRR4302535.3329402/94‑1 (MQ=60)

                                                                             CTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCC                  >  SRR4302535.2191109/1‑94 (MQ=60)

                                                                             CTTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCC                  >  SRR4302535.3975722/1‑94 (MQ=60)

                                                                              TTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCA                 >  SRR4302535.3895554/1‑94 (MQ=60)

                                                                              TTTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCA                 >  SRR4302535.347360/1‑94 (MQ=60)

                                                                               TTCAGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAA                >  SRR4302535.618677/1‑94 (MQ=60)

                                                                                  AGCGTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTT             >  SRR4302535.1441965/1‑94 (MQ=60)

                                                                                     GTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTAT          >  SRR4302535.3398068/1‑94 (MQ=60)

                                                                                     GTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTAT          <  SRR4302535.4047173/94‑1 (MQ=60)

                                                                                     GTGCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTAT          <  SRR4302535.56589/94‑1 (MQ=60)

                                                                                       GCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTC        <  SRR4302535.3839975/94‑1 (MQ=60)

                                                                                       GCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTC        <  SRR4302535.4151910/94‑1 (MQ=60)

                                                                                       GCGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTC        <  SRR4302535.3797469/94‑1 (MQ=60)

                                                                                        CGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCG       >  SRR4302535.3166457/1‑94 (MQ=60)

                                                                                        CGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCG       >  SRR4302535.3913057/1‑94 (MQ=60)

                                                                                        CGAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCG       >  SRR4302535.4556270/1‑94 (MQ=60)

                                                                                         GAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGC      >  SRR4302535.3609138/1‑94 (MQ=60)

                                                                                         GAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGC      >  SRR4302535.3281469/1‑94 (MQ=60)

                                                                                         GAATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGA      >  SRR4302535.3221920/1‑94 (MQ=60)

                                                                                          AATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGTAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCC     <  SRR4302535.2179770/94‑1 (MQ=60)

                                                                                          AATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCC     >  SRR4302535.4091420/1‑94 (MQ=60)

                                                                                          AATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCC     <  SRR4302535.3963465/94‑1 (MQ=60)

                                                                                           ATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCA    <  SRR4302535.3065833/94‑1 (MQ=60)

                                                                                           ATGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCA    <  SRR4302535.1478877/94‑1 (MQ=60)

                                                                                            TGCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAG   <  SRR4302535.381786/94‑1 (MQ=60)

                                                                                             GCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAGC  >  SRR4302535.4308044/1‑94 (MQ=60)

                                                                                             GCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAGC  <  SRR4302535.4357812/94‑1 (MQ=60)

                                                                                             GCGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAGC  <  SRR4302535.1296576/94‑1 (MQ=60)

AATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCACCGCTTTGGCTTTGATATAGCCACGGTTGGAGGAGACTTTCACGGTATCGCCCTGGGCAATGCCAAGTTTATTCGCCAGC  >  NC_000913/4080439‑4080625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`