Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,675	Δ1 bp	intergenic (+38/+13)	yiiG → / ← frvR	hypothetical protein/putative frv operon regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,675	0	G	.	100.0%	868.0 / NA	191	intergenic (+38/+13)	yiiG/frvR	hypothetical protein/putative frv operon regulatory protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (123/68); total (123/68)

Only 100 of 215 total aligned reads displayed.

GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762

gCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCg                                                                                    >  1:732291/1‑93 (MQ=255)

 cATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGa                                                                                   >  2:924812/1‑93 (MQ=255)

   ttACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca                                                                                 >  2:1412624/1‑93 (MQ=255)

   ttACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca                                                                                 >  1:4111954/1‑93 (MQ=255)

    tACGCAAATAAAACAAAATTGTTAATGATTATAATAGCCTGCGTTAATTT‑TTTTTAATAAAAAAGGCAGTGTCGGAGGCGGCGC‑ACCCGacac                                                                                <  2:856498/93‑1 (MQ=255)

     aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               >  2:1276049/1‑93 (MQ=255)

     aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               <  1:597748/93‑1 (MQ=255)

       gcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt                                                                             <  2:2621732/93‑1 (MQ=255)

       gcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt                                                                             >  1:293632/1‑93 (MQ=255)

       gcgAGTACAACGAAAGGGTTGATGATTATAATAGCCTGCGTTAAACT‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt                                                                             <  2:3211385/93‑1 (MQ=255)

         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           <  2:2093182/93‑1 (MQ=255)

         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           >  2:1830743/1‑93 (MQ=255)

         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           >  1:4226566/1‑93 (MQ=255)

          aggaCAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGCGTCGGATGCGGCG‑TCTCCGACACAGTTCa                                                                          >  2:4655234/4‑93 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTcc                                                                          >  2:3130569/1‑92 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCa                                                                          >  1:2146803/1‑93 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGAAGTGTCGGATGCGGCGC‑ATCCGACACAGTTcc                                                                          >  1:3433200/1‑92 (MQ=255)

           gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAc                                                                         >  1:519490/1‑93 (MQ=255)

           gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAc                                                                         >  1:3163600/1‑93 (MQ=255)

            tACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcc                                                                        <  1:221857/93‑1 (MQ=255)

             aCAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccc                                                                       >  2:431568/1‑93 (MQ=255)

              cAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc                                                                      >  2:1909710/1‑93 (MQ=255)

               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccc                                                                     >  1:3672460/1‑92 (MQ=255)

               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccc                                                                     >  1:2614074/1‑92 (MQ=255)

               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccc                                                                     >  2:4406428/1‑92 (MQ=255)

                aCGAAATGTTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ACCCGACACAGTTCAccccct                                                                    <  2:2720158/93‑3 (MQ=255)

                aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccct                                                                    >  2:2425285/1‑91 (MQ=255)

                aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccct                                                                    >  1:2112083/1‑91 (MQ=255)

                  gAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgt                                                                  >  2:4413312/1‑93 (MQ=255)

                    aaTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGa                                                                >  2:2497191/1‑93 (MQ=255)

                     aTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:405776/93‑1 (MQ=255)

                     aTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:3814494/93‑1 (MQ=255)

                      tGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                              <  1:3694819/93‑1 (MQ=255)

                       ggTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGt                                                             >  1:453854/1‑93 (MQ=255)

                          tgatgaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAc                                                          >  1:4636185/1‑93 (MQ=255)

                          tgatgaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAc                                                          >  1:1341988/1‑93 (MQ=255)

                             tgaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTcc                                                       >  2:1505633/1‑93 (MQ=255)

                              gaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTCACTCCa                                                      >  1:4005056/1‑93 (MQ=255)

                               aCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     <  1:1927065/93‑1 (MQ=255)

                                cTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                    <  1:3786470/93‑1 (MQ=255)

                                cTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                    >  2:4474936/1‑93 (MQ=255)

                                 tataATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                   >  1:2553543/1‑93 (MQ=255)

                                  aaaatgGCCTGC‑TTAAACGTTTTTTGATGAAAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  <  2:1720452/91‑1 (MQ=255)

                                   taataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                 <  2:2670433/93‑1 (MQ=255)

                                   taataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                 <  2:3620739/93‑1 (MQ=255)

                                   taataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                 >  1:2011068/1‑93 (MQ=255)

                                        gCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTc                                            >  1:3903152/1‑93 (MQ=255)

                                        gCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTc                                            <  1:1192263/93‑1 (MQ=255)

                                         ccTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCa                                           <  1:3471869/93‑1 (MQ=255)

                                           tGCGTTAACCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         >  2:4568581/1‑93 (MQ=255)

                                           tGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGCAGTAACTCCATAAATGCCTCata                                         >  2:3537526/1‑93 (MQ=255)

                                           tGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         >  2:3432876/1‑93 (MQ=255)

                                           tGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         >  1:2035415/1‑93 (MQ=255)

                                             cGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       <  1:3087489/93‑1 (MQ=255)

                                             cGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       <  2:3867406/93‑1 (MQ=255)

                                              gTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATcc                                      >  1:3056393/1‑93 (MQ=255)

                                              gTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATcc                                      <  1:1861167/93‑1 (MQ=255)

                                               ttAAACT‑TTTTGGAGAATAAAGGCGGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     <  2:2564357/93‑1 (MQ=255)

                                               ttAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     >  2:2229190/1‑93 (MQ=255)

                                               ttAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     <  1:2258375/93‑1 (MQ=255)

                                                 aaaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                   <  1:1777950/93‑1 (MQ=255)

                                                  aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  1:1999376/1‑93 (MQ=255)

                                                  aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  1:4413657/1‑93 (MQ=255)

                                                  aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  2:3339382/1‑93 (MQ=255)

                                                  aaCG‑TTTTTGATAATAAATGCAGTGTCGGAGGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  <  2:811844/93‑1 (MQ=255)

                                                   aCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTa                                 >  1:3060180/1‑93 (MQ=255)

                                                   aCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTa                                 >  1:3630753/1‑93 (MQ=255)

                                                    cG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                >  1:1231329/1‑93 (MQ=255)

                                                    cG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                >  2:3156936/1‑93 (MQ=255)

                                                     g‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTTACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               >  1:3253268/1‑93 (MQ=255)

                                                       tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              <  1:382575/93‑1 (MQ=255)

                                                       tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              >  2:2022963/1‑93 (MQ=255)

                                                        ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                             >  2:3659381/1‑93 (MQ=255)

                                                        ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                             <  2:407541/93‑1 (MQ=255)

                                                             atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTACATAAATGCCTCATATCCTGTTAACCCGGCaa                        >  1:2342117/1‑93 (MQ=255)

                                                              tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                       <  2:2293015/93‑1 (MQ=255)

                                                              tgatAAATGCAGTGTCGGATGCGGC‑CCACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                       <  1:1625416/93‑1 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  2:741081/93‑1 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  1:1420820/93‑1 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  2:553018/93‑1 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  1:3334602/1‑93 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  2:4232741/1‑93 (MQ=255)

                                                               gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  2:419079/1‑93 (MQ=255)

                                                                atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                     >  1:312463/1‑93 (MQ=255)

                                                                 tAAATGCAGTGTCGGATGCGGCGCG‑TCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    <  2:4156785/93‑1 (MQ=255)

                                                                 tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    >  1:417376/1‑93 (MQ=255)

                                                                 tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    <  1:4150128/93‑1 (MQ=255)

                                                                   aaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtct                  <  1:1030548/93‑1 (MQ=255)

                                                                    aTGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  1:990520/93‑1 (MQ=255)

                                                                     tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                <  2:4545797/93‑1 (MQ=255)

                                                                     tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  2:4621521/1‑93 (MQ=255)

                                                                     tGCAGTGTCGGAGGCGCCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                <  2:3941235/93‑1 (MQ=255)

                                                                            tCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt         <  1:154855/93‑1 (MQ=255)

                                                                              ggATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGc       <  2:3764903/93‑1 (MQ=255)

                                                                              ggATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGc       <  1:2432943/93‑1 (MQ=255)

                                                                              ggAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGc       <  1:3184596/93‑1 (MQ=255)

                                                                               gATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      >  1:2238874/1‑93 (MQ=255)

                                                                               gATGCGGCGC‑ATCCGACACAGTTCACACCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGCTGcg      >  1:3416690/1‑92 (MQ=255)

                                                                               gATGCGGCGC‑ATCCGAAACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      <  2:2394829/93‑1 (MQ=255)

                                                                                 tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    >  2:3969005/1‑93 (MQ=255)

GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA  >  NC_000913/4085582‑4085764

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCA                                                                                           >  SRR4302535.2306870/1‑94 (MQ=60)

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCA                                                                                           >  SRR4302535.4345856/1‑94 (MQ=60)

  AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATC                                                                                        >  SRR4302535.3595404/1‑94 (MQ=60)

  AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATC                                                                                        >  SRR4302535.3558115/1‑94 (MQ=60)

   GAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCC                                                                                       <  SRR4302535.1731285/94‑1 (MQ=60)

    AGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCG                                                                                      <  SRR4302535.2576114/94‑1 (MQ=60)

    AGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCG                                                                                      >  SRR4302535.737432/1‑94 (MQ=60)

     GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGA                                                                                     <  SRR4302535.3121111/94‑1 (MQ=60)

       ATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACA                                                                                   >  SRR4302535.950098/1‑94 (MQ=60)

       ATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACA                                                                                   >  SRR4302535.4140097/1‑94 (MQ=60)

       ATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACA                                                                                   >  SRR4302535.1266707/1‑94 (MQ=60)

          ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                <  SRR4302535.601931/94‑1 (MQ=60)

           CGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGT                                                                               >  SRR4302535.295684/1‑94 (MQ=60)

             CGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTC                                                                             >  SRR4302535.4255489/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                            >  SRR4302535.2162245/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                            <  SRR4302535.2017931/94‑1 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGAAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCC                                                                            >  SRR4302535.3457055/1‑94 (MQ=60)

               AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAC                                                                           >  SRR4302535.523125/1‑94 (MQ=60)

               AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAC                                                                           >  SRR4302535.3185539/1‑94 (MQ=60)

                GTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACC                                                                          <  SRR4302535.3807708/94‑1 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                         <  SRR4302535.4199145/94‑1 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                         <  SRR4302535.223384/94‑1 (MQ=60)

                  ACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCC                                                                        >  SRR4302535.2542982/1‑94 (MQ=60)

                   CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCC                                                                       >  SRR4302535.3697891/1‑94 (MQ=60)

                   CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCC                                                                       >  SRR4302535.2632822/1‑94 (MQ=60)

                    AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCT                                                                      >  SRR4302535.2127330/1‑94 (MQ=60)

                     ACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTG                                                                     <  SRR4302535.3710258/94‑1 (MQ=60)

                      CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGT                                                                    <  SRR4302535.1830910/94‑1 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                   <  SRR4302535.3520115/94‑1 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                   >  SRR4302535.4362405/1‑94 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGCTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                   >  SRR4302535.3687535/1‑94 (MQ=60)

                         AATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAA                                                                 >  SRR4302535.2161634/1‑94 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                <  SRR4302535.3840813/94‑1 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                >  SRR4302535.3101137/1‑94 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                <  SRR4302535.408617/94‑1 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                               >  SRR4302535.1882052/1‑94 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                               >  SRR4302535.457035/1‑94 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                               >  SRR4302535.1701320/1‑94 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                               <  SRR4302535.3720393/94‑1 (MQ=60)

                             GTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAA                                                             >  SRR4302535.3896404/1‑94 (MQ=60)

                              TTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                            >  SRR4302535.4667633/1‑94 (MQ=60)

                              TTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                            >  SRR4302535.1351564/1‑94 (MQ=60)

                               TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                           >  SRR4302535.4178432/1‑94 (MQ=60)

                                  TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTCACTCCA                                                        >  SRR4302535.4032451/1‑94 (MQ=60)

                                  TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCA                                                        >  SRR4302535.2478892/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                       >  SRR4302535.2261286/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAAGGCAGTGTCGGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                       <  SRR4302535.1726327/94‑1 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                      >  SRR4302535.4504947/1‑94 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                      <  SRR4302535.1940954/94‑1 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                     >  SRR4302535.2571928/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                     >  SRR4302535.3460377/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                     <  SRR4302535.3812589/94‑1 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                    >  SRR4302535.2032127/1‑94 (MQ=60)

                                       ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                   >  SRR4302535.2025544/1‑94 (MQ=60)

                                       ATAAGAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                   <  SRR4302535.2003705/94‑1 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                >  SRR4302535.2236375/1‑94 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                >  SRR4302535.3148987/1‑94 (MQ=60)

                                           aagccgggGTTAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                               <  SRR4302535.1724581/86‑1 (MQ=60)

                                           TAGCCTGCTTTAAACTTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                               <  SRR4302535.4036908/94‑1 (MQ=60)

                                           TAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                               <  SRR4302535.1213710/94‑1 (MQ=60)

                                            AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTC                                              >  SRR4302535.3929890/1‑94 (MQ=60)

                                             GCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCA                                             <  SRR4302535.1200802/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCTGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                            <  SRR4302535.3324255/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                            <  SRR4302535.3420369/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                            >  SRR4302535.468003/1‑94 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                            <  SRR4302535.3495993/94‑1 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                           >  SRR4302535.2050078/1‑94 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                           >  SRR4302535.2083525/1‑94 (MQ=60)

                                                  CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                        <  SRR4302535.3108994/94‑1 (MQ=60)

                                                  CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                        >  SRR4302535.3077784/1‑94 (MQ=60)

                                                   GTTAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCT                                       <  SRR4302535.1874589/94‑1 (MQ=60)

                                                   GTT‑AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                      <  SRR4302535.3201900/94‑1 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                      <  SRR4302535.1512436/94‑1 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                      <  SRR4302535.2274454/94‑1 (MQ=60)

                                                     TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                     >  SRR4302535.1149450/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                    >  SRR4302535.2013778/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                    >  SRR4302535.4443863/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                    <  SRR4302535.1575167/94‑1 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                    >  SRR4302535.4443864/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                    <  SRR4302535.1790827/94‑1 (MQ=60)

                                                       AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                   >  SRR4302535.3655922/1‑94 (MQ=60)

                                                       AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                   >  SRR4302535.3081584/1‑94 (MQ=60)

                                                        ACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAA                                  >  SRR4302535.1240134/1‑94 (MQ=60)

                                                         CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTTACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                 >  SRR4302535.3275871/1‑94 (MQ=60)

                                                          GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                >  SRR4302535.2231339/1‑94 (MQ=60)

                                                           TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                               >  SRR4302535.4248309/1‑94 (MQ=60)

                                                           TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                               >  SRR4302535.3215290/1‑94 (MQ=60)

                                                           TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                               <  SRR4302535.385223/94‑1 (MQ=60)

                                                            TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                              <  SRR4302535.2284716/94‑1 (MQ=60)

                                                            TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                              >  SRR4302535.560522/1‑94 (MQ=60)

                                                               TGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                           <  SRR4302535.3441650/94‑1 (MQ=60)

                                                                GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                          >  SRR4302535.2767801/1‑94 (MQ=60)

                                                                GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTACATAAATGCCTCATATCCTGTTAACCCGGCAA                          >  SRR4302535.2358874/1‑94 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                         <  SRR4302535.3914104/94‑1 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                         >  SRR4302535.2160961/1‑94 (MQ=60)

                                                                  TGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                        >  SRR4302535.3357828/1‑94 (MQ=60)

                                                                  TGATAAATGCAGTGTCGGATGCGGC‑CCACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                        <  SRR4302535.1637151/94‑1 (MQ=60)

                                                                   GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                       >  SRR4302535.314627/1‑94 (MQ=60)

                                                                   GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                       <  SRR4302535.1430964/94‑1 (MQ=60)

                                                                    ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                      >  SRR4302535.420302/1‑94 (MQ=60)

                                                                    ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                      >  SRR4302535.4093526/1‑94 (MQ=60)

                                                                    ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                      <  SRR4302535.3610576/94‑1 (MQ=60)

                                                                     TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                     >  SRR4302535.4581672/1‑94 (MQ=60)

                                                                     TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                     <  SRR4302535.4178547/94‑1 (MQ=60)

                                                                      AAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                    <  SRR4302535.3826304/94‑1 (MQ=60)

                                                                       AATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTC                   <  SRR4302535.1037865/94‑1 (MQ=60)

                                                                        ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                  <  SRR4302535.260268/94‑1 (MQ=60)

                                                                        ATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                  <  SRR4302535.997553/94‑1 (MQ=60)

                                                                         TGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCG                 >  SRR4302535.3940019/1‑94 (MQ=60)

                                                                            AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG              >  SRR4302535.611222/1‑94 (MQ=60)

                                                                              TGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGA            >  SRR4302535.3159071/1‑94 (MQ=60)

                                                                                TCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATG          <  SRR4302535.155943/94‑1 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT        >  SRR4302535.2254804/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACACCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGctgcg        >  SRR4302535.3440458/1‑89 (MQ=60)

                                                                                  GGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT        <  SRR4302535.2450469/94‑1 (MQ=60)

                                                                                  GGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT        <  SRR4302535.3206610/94‑1 (MQ=60)

                                                                                   GATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG       >  SRR4302535.3844561/1‑94 (MQ=60)

                                                                                   GATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG       <  SRR4302535.3080741/94‑1 (MQ=60)

                                                                                     TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC     >  SRR4302535.1386855/1‑94 (MQ=60)

                                                                                       CGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACAC   <  SRR4302535.303699/94‑1 (MQ=60)

                                                                                        GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA  <  SRR4302535.3570594/94‑1 (MQ=60)

                                                                                        GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA  >  SRR4302535.3474140/1‑94 (MQ=60)

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA  >  NC_000913/4085582‑4085764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`