Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,695	T→C	K581R (AAG→AGG)	frvR ←	putative frv operon regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,695	0	T	C	100.0%	677.4 / NA	213	K581R (AAG→AGG)	frvR	putative frv operon regulatory protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (113/100); total (113/100)

Only 100 of 218 total aligned reads displayed.

CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTA  >  NC_000913/4085604‑4085784

cGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtg                                                                                         <  1:1817774/93‑1 (MQ=255)

 gAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgt                                                                                        >  2:4413312/1‑93 (MQ=255)

  aaaTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                                       >  2:1157780/1‑93 (MQ=255)

  aaaTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                                       >  1:4332774/1‑93 (MQ=255)

  aaaTGGTTGATGACTATAATAGCCTGCGTTAAACG‑CTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                                       >  1:3662177/1‑93 (MQ=255)

    aTGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                                                     <  1:3814494/93‑1 (MQ=255)

     tGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                                                    >  1:3079664/1‑93 (MQ=255)

     tGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                                                    >  2:3960366/1‑93 (MQ=255)

      ggTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGt                                                                                   >  1:1689096/1‑93 (MQ=255)

      ggTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGt                                                                                   >  1:1868581/1‑93 (MQ=255)

         tgatgaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAc                                                                                >  1:1341988/1‑93 (MQ=255)

            tgaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTcc                                                                             >  2:1505633/1‑93 (MQ=255)

             gaCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTCACTCCa                                                                            >  1:4005056/1‑93 (MQ=255)

              aCTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                                           >  1:2245321/1‑93 (MQ=255)

               cTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                                          >  2:4474936/1‑93 (MQ=255)

               cTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                                          <  1:3786470/93‑1 (MQ=255)

               cTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                                          >  1:4474246/1‑93 (MQ=255)

                tataATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                                         >  2:4303165/1‑93 (MQ=255)

                tataATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                                         >  2:2417969/1‑93 (MQ=255)

                tataATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                                         >  1:2553543/1‑93 (MQ=255)

                 ataataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                                        >  1:2017592/1‑93 (MQ=255)

                 aaaatgGCCTGC‑TTAAACGTTTTTTGATGAAAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                                        <  2:1720452/91‑1 (MQ=255)

                  taataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                                       <  2:3620739/93‑1 (MQ=255)

                  taataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                                       <  2:432382/93‑1 (MQ=255)

                  aaataGCTTGCTTAAAACG‑TTTTGAATAAAAATGGCGGTGTGGAATG‑GGCCCCACCCAACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                                       <  1:2414405/92‑1 (MQ=255)

                   aataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                                      <  2:2697245/93‑1 (MQ=255)

                    ataGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGc                                                                     <  2:927382/93‑1 (MQ=255)

                     taGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGcc                                                                    <  1:1205054/93‑1 (MQ=255)

                       gCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTc                                                                  >  2:1336487/1‑93 (MQ=255)

                       gCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTc                                                                  >  1:3903152/1‑93 (MQ=255)

                        ccTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCa                                                                 <  1:3396737/93‑1 (MQ=255)

                          tGCGTTAACCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                                               >  2:4568581/1‑93 (MQ=255)

                          tGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                                               >  1:2068614/1‑93 (MQ=255)

                            cGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCTTCATATc                                                             >  2:2952766/1‑93 (MQ=255)

                             gTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATcc                                                            >  2:231881/1‑93 (MQ=255)

                              ttAAACT‑TTTTGGAGAATAAAGGCGGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                                           <  2:2564357/93‑1 (MQ=255)

                              ttAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                                           >  2:2229190/1‑93 (MQ=255)

                               tAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                                          >  2:3870840/1‑93 (MQ=255)

                                aaaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                                         >  1:1141315/1‑93 (MQ=255)

                                 aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                                        >  2:3494912/1‑93 (MQ=255)

                                 aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                                        >  1:1999376/1‑93 (MQ=255)

                                 aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                                        >  2:3339382/1‑93 (MQ=255)

                                 aaCG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                                        >  1:4413656/1‑93 (MQ=255)

                                 aaCG‑TTTTTGATAATAAATGCAGTGTCGGAGGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                                        <  2:811844/93‑1 (MQ=255)

                                   cG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                                      >  2:381035/1‑93 (MQ=255)

                                   cG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                                      >  2:3156936/1‑93 (MQ=255)

                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                                                    >  1:2215563/1‑93 (MQ=255)

                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                                                    >  2:2022963/1‑93 (MQ=255)

                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                                                    <  1:382575/93‑1 (MQ=255)

                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                                                   <  1:2268545/93‑1 (MQ=255)

                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                                                   <  2:407541/93‑1 (MQ=255)

                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                                                   >  1:3193198/1‑93 (MQ=255)

                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCATGTTAAccc                                                   >  2:3764257/1‑93 (MQ=255)

                                          tgatgaTAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                                                <  1:3417874/93‑1 (MQ=255)

                                           gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                                               >  2:4180678/1‑93 (MQ=255)

                                            atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa                                              >  1:2748212/1‑93 (MQ=255)

                                             tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                                             >  1:2145525/1‑93 (MQ=255)

                                              gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                                            >  1:3334602/1‑93 (MQ=255)

                                              gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                                            <  2:741081/93‑1 (MQ=255)

                                               atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                                           >  1:312463/1‑93 (MQ=255)

                                               atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                                           <  1:3585702/93‑1 (MQ=255)

                                                tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                                          <  1:4150128/93‑1 (MQ=255)

                                                 aaaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtc                                         >  1:4550568/1‑93 (MQ=255)

                                                 aaaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtc                                         <  2:4216057/93‑1 (MQ=255)

                                                  aaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtct                                        <  1:1030548/93‑1 (MQ=255)

                                                   aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                                       >  2:2655682/1‑93 (MQ=255)

                                                   aTGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                                       <  1:990520/93‑1 (MQ=255)

                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                                      >  2:4621521/1‑93 (MQ=255)

                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                                      <  2:4545797/93‑1 (MQ=255)

                                                        gtgtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgccg                                  <  2:3843258/93‑1 (MQ=255)

                                                          gtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGa                                >  2:3411611/1‑93 (MQ=255)

                                                          gtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGa                                >  1:3137239/1‑93 (MQ=255)

                                                           tCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt                               <  1:154855/93‑1 (MQ=255)

                                                              gATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt                            <  1:3059343/93‑1 (MQ=255)

                                                                tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa                          <  2:696408/93‑1 (MQ=255)

                                                                 gcggcgC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGac                         >  1:1377026/1‑93 (MQ=255)

                                                                     gcgcaTCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAcaca                      >  1:3450157/5‑93 (MQ=255)

                                                                       gcaTCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAat                    <  1:1457273/91‑1 (MQ=255)

                                                                       gcaTCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAat                    <  1:4124392/91‑1 (MQ=255)

                                                                       gcaTCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAat                    <  1:545191/91‑1 (MQ=255)

                                                                        caTCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAata                   >  2:3573416/2‑93 (MQ=255)

                                                                         acccGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAatat                  <  2:19692/91‑1 (MQ=255)

                                                                          tCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                 >  1:4022464/1‑93 (MQ=255)

                                                                          tCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                 >  1:4621071/1‑93 (MQ=255)

                                                                          tCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                 >  1:599394/1‑93 (MQ=255)

                                                                          tCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                 >  1:803322/1‑93 (MQ=255)

                                                                          tCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                 <  1:3266058/93‑1 (MQ=255)

                                                                            cGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCAGCAATAATCTCCGCCGGATGCTGACACAATATGCt               <  2:85632/93‑1 (MQ=255)

                                                                             gACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCtt              >  1:4678405/1‑93 (MQ=255)

                                                                             gACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCtt              <  1:1610409/93‑1 (MQ=255)

                                                                             gACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCtt              <  2:2252758/93‑1 (MQ=255)

                                                                              acacAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTg             <  2:4265524/93‑1 (MQ=255)

                                                                                acaGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGcc           <  1:3027119/93‑1 (MQ=255)

                                                                                   gTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGa        >  1:1759433/1‑93 (MQ=255)

                                                                                   gTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGa        <  1:2453137/93‑1 (MQ=255)

                                                                                   gTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGa        >  2:4656287/1‑93 (MQ=255)

                                                                                    ttCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGAt       >  2:2498499/1‑93 (MQ=255)

                                                                                        ccccCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCt   <  1:4059075/93‑1 (MQ=255)

                                                                                         ccccTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTa  >  2:1633421/1‑93 (MQ=255)

                                                                                         ccccTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTa  <  2:1855541/93‑1 (MQ=255)

CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACG‑TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTA  >  NC_000913/4085604‑4085784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATA  >  NC_000913/4085601‑4085788

CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCC                                                                                               >  SRR4302535.3697891/1‑94 (MQ=60)

CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCC                                                                                               >  SRR4302535.2632822/1‑94 (MQ=60)

 AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCT                                                                                              >  SRR4302535.2127330/1‑94 (MQ=60)

  ACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTG                                                                                             <  SRR4302535.3710258/94‑1 (MQ=60)

   CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGT                                                                                            <  SRR4302535.1830910/94‑1 (MQ=60)

    GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                                           >  SRR4302535.4362405/1‑94 (MQ=60)

    GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                                           <  SRR4302535.3520115/94‑1 (MQ=60)

    GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGCTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                                           >  SRR4302535.3687535/1‑94 (MQ=60)

      AATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAA                                                                                         >  SRR4302535.2161634/1‑94 (MQ=60)

       ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                                        >  SRR4302535.3101137/1‑94 (MQ=60)

       ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                                        <  SRR4302535.408617/94‑1 (MQ=60)

       ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                                        <  SRR4302535.3840813/94‑1 (MQ=60)

        TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                                                       >  SRR4302535.457035/1‑94 (MQ=60)

        TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                                                       >  SRR4302535.1882052/1‑94 (MQ=60)

        TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                                                       >  SRR4302535.1701320/1‑94 (MQ=60)

        TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                                                       <  SRR4302535.3720393/94‑1 (MQ=60)

          GTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAA                                                                                     >  SRR4302535.3896404/1‑94 (MQ=60)

           TTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                                                    >  SRR4302535.4667633/1‑94 (MQ=60)

           TTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                                                    >  SRR4302535.1351564/1‑94 (MQ=60)

            TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                                                   >  SRR4302535.4178432/1‑94 (MQ=60)

               TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTCACTCCA                                                                                >  SRR4302535.4032451/1‑94 (MQ=60)

               TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCA                                                                                >  SRR4302535.2478892/1‑94 (MQ=60)

                GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                                               >  SRR4302535.2261286/1‑94 (MQ=60)

                GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAAGGCAGTGTCGGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                                               <  SRR4302535.1726327/94‑1 (MQ=60)

                 ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                                              >  SRR4302535.4504947/1‑94 (MQ=60)

                 ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                                              <  SRR4302535.1940954/94‑1 (MQ=60)

                  CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                                             >  SRR4302535.2571928/1‑94 (MQ=60)

                  CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                                             <  SRR4302535.3812589/94‑1 (MQ=60)

                  CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                                             >  SRR4302535.3460377/1‑94 (MQ=60)

                   TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                                            >  SRR4302535.2032127/1‑94 (MQ=60)

                    ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                                           >  SRR4302535.2025544/1‑94 (MQ=60)

                    ATAAGAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                                           <  SRR4302535.2003705/94‑1 (MQ=60)

                      aaatagcttgcttaaaacgttttgaataaaaatggcggtgtggaatgggccccacccaACACAGTTCACCCCCTGTGAAGTAACTCCATAAATG                                                                          <  SRR4302535.2431784/36‑1 (MQ=60)

                       ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                                        >  SRR4302535.3148987/1‑94 (MQ=60)

                       ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                                        >  SRR4302535.2236375/1‑94 (MQ=60)

                        aagccgggGTTAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                                                       <  SRR4302535.1724581/86‑1 (MQ=60)

                        TAGCCTGCTTTAAACTTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                                                       <  SRR4302535.4036908/94‑1 (MQ=60)

                        TAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                                                       <  SRR4302535.1213710/94‑1 (MQ=60)

                         AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTC                                                                      >  SRR4302535.3929890/1‑94 (MQ=60)

                          GCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCA                                                                     <  SRR4302535.1200802/94‑1 (MQ=60)

                           CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCTGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                                    <  SRR4302535.3324255/94‑1 (MQ=60)

                           CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                                    >  SRR4302535.468003/1‑94 (MQ=60)

                           CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                                    <  SRR4302535.3420369/94‑1 (MQ=60)

                           CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                                    <  SRR4302535.3495993/94‑1 (MQ=60)

                            CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                                                   >  SRR4302535.2050078/1‑94 (MQ=60)

                            CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                                                   >  SRR4302535.2083525/1‑94 (MQ=60)

                               CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                                                >  SRR4302535.3077784/1‑94 (MQ=60)

                               CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                                                <  SRR4302535.3108994/94‑1 (MQ=60)

                                GTTAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCT                                                               <  SRR4302535.1874589/94‑1 (MQ=60)

                                GTT‑AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                                              <  SRR4302535.3201900/94‑1 (MQ=60)

                                 TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                                              <  SRR4302535.1512436/94‑1 (MQ=60)

                                 TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                                              <  SRR4302535.2274454/94‑1 (MQ=60)

                                  TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                                             >  SRR4302535.1149450/1‑94 (MQ=60)

                                   AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                                            >  SRR4302535.4443864/1‑94 (MQ=60)

                                   AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                                            <  SRR4302535.1790827/94‑1 (MQ=60)

                                   AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                                            <  SRR4302535.1575167/94‑1 (MQ=60)

                                   AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                                            >  SRR4302535.2013778/1‑94 (MQ=60)

                                   AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                                            >  SRR4302535.4443863/1‑94 (MQ=60)

                                    AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                                           >  SRR4302535.3655922/1‑94 (MQ=60)

                                    AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                                           >  SRR4302535.3081584/1‑94 (MQ=60)

                                     ACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAA                                                          >  SRR4302535.1240134/1‑94 (MQ=60)

                                      CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTTACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                                         >  SRR4302535.3275871/1‑94 (MQ=60)

                                       GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                                        >  SRR4302535.2231339/1‑94 (MQ=60)

                                        TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                                       >  SRR4302535.3215290/1‑94 (MQ=60)

                                        TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                                       <  SRR4302535.385223/94‑1 (MQ=60)

                                        TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                                       >  SRR4302535.4248309/1‑94 (MQ=60)

                                         TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                                      >  SRR4302535.560522/1‑94 (MQ=60)

                                         TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                                      <  SRR4302535.2284716/94‑1 (MQ=60)

                                            TGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                                                   <  SRR4302535.3441650/94‑1 (MQ=60)

                                             GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                                                  >  SRR4302535.2767801/1‑94 (MQ=60)

                                             GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTACATAAATGCCTCATATCCTGTTAACCCGGCAA                                                  >  SRR4302535.2358874/1‑94 (MQ=60)

                                              gataaaaaaggcagggtgggaggcggcccacccgcCACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                                                  <  SRR4302535.1667329/59‑1 (MQ=60)

                                              ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                                                 <  SRR4302535.3914104/94‑1 (MQ=60)

                                              ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                                                 >  SRR4302535.2160961/1‑94 (MQ=60)

                                               TGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                                                >  SRR4302535.3357828/1‑94 (MQ=60)

                                               TGATAAATGCAGTGTCGGATGCGGC‑CCACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                                                <  SRR4302535.1637151/94‑1 (MQ=60)

                                                GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                                               <  SRR4302535.1430964/94‑1 (MQ=60)

                                                GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                                               >  SRR4302535.314627/1‑94 (MQ=60)

                                                 ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                                              >  SRR4302535.4093526/1‑94 (MQ=60)

                                                 ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                                              >  SRR4302535.420302/1‑94 (MQ=60)

                                                 ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                                              <  SRR4302535.3610576/94‑1 (MQ=60)

                                                  TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                                             <  SRR4302535.4178547/94‑1 (MQ=60)

                                                  TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                                             >  SRR4302535.4581672/1‑94 (MQ=60)

                                                   AAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                                            <  SRR4302535.3826304/94‑1 (MQ=60)

                                                    AATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTC                                           <  SRR4302535.1037865/94‑1 (MQ=60)

                                                     ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                                          <  SRR4302535.260268/94‑1 (MQ=60)

                                                     ATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                                          <  SRR4302535.997553/94‑1 (MQ=60)

                                                      TGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCG                                         >  SRR4302535.3940019/1‑94 (MQ=60)

                                                         AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                                      >  SRR4302535.611222/1‑94 (MQ=60)

                                                           TGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGA                                    >  SRR4302535.3159071/1‑94 (MQ=60)

                                                             tccggagccgccccacCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT                                   <  SRR4302535.125215/78‑1 (MQ=60)

                                                             TCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATG                                  <  SRR4302535.155943/94‑1 (MQ=60)

                                                               GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT                                >  SRR4302535.2254804/1‑94 (MQ=60)

                                                               GGATGCGGCGC‑ATCCGACACAGTTCACACCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGctgcg                                >  SRR4302535.3440458/1‑89 (MQ=60)

                                                               GGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT                                <  SRR4302535.2450469/94‑1 (MQ=60)

                                                               GGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT                                <  SRR4302535.3206610/94‑1 (MQ=60)

                                                                GATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG                               >  SRR4302535.3844561/1‑94 (MQ=60)

                                                                GATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG                               <  SRR4302535.3080741/94‑1 (MQ=60)

                                                                  TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC                             >  SRR4302535.1386855/1‑94 (MQ=60)

                                                                    CGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACAC                           <  SRR4302535.303699/94‑1 (MQ=60)

                                                                     GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA                          >  SRR4302535.3474140/1‑94 (MQ=60)

                                                                     GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA                          <  SRR4302535.3570594/94‑1 (MQ=60)

                                                                      ggcccATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA                          <  SRR4302535.87990/89‑1 (MQ=60)

                                                                         gcATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATA                       <  SRR4302535.549001/92‑1 (MQ=60)

                                                                         gcATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATA                       <  SRR4302535.1467699/92‑1 (MQ=60)

                                                                         gcATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATA                       <  SRR4302535.4152619/92‑1 (MQ=60)

                                                                           ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATG                     >  SRR4302535.603586/1‑94 (MQ=60)

                                                                           ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATG                     >  SRR4302535.4049985/1‑94 (MQ=60)

                                                                           ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATG                     >  SRR4302535.808963/1‑94 (MQ=60)

                                                                           ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATG                     >  SRR4302535.4652467/1‑94 (MQ=60)

                                                                            TCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGC                    <  SRR4302535.2157650/94‑1 (MQ=60)

                                                                            TCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGC                    <  SRR4302535.3288748/94‑1 (MQ=60)

                                                                              CGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTT                  >  SRR4302535.4709971/1‑94 (MQ=60)

                                                                              CGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTT                  <  SRR4302535.2781929/94‑1 (MQ=60)

                                                                               GACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTG                 <  SRR4302535.4617350/94‑1 (MQ=60)

                                                                               GACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTG                 <  SRR4302535.1101603/94‑1 (MQ=60)

                                                                               GACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTG                 <  SRR4302535.1622016/94‑1 (MQ=60)

                                                                                 CACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCC               >  SRR4302535.1550955/1‑94 (MQ=60)

                                                                                  ACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCA              <  SRR4302535.3048312/94‑1 (MQ=60)

                                                                                   CAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAG             <  SRR4302535.478686/94‑1 (MQ=60)

                                                                                    AGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGA            >  SRR4302535.1772172/1‑94 (MQ=60)

                                                                                     GTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGAT           <  SRR4302535.83638/94‑1 (MQ=60)

                                                                                     GTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGAT           <  SRR4302535.2470817/94‑1 (MQ=60)

                                                                                       TCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAG         <  SRR4302535.1367749/94‑1 (MQ=60)

                                                                                         ACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCT       <  SRR4302535.4433471/94‑1 (MQ=60)

                                                                                          CCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTA      <  SRR4302535.3361705/94‑1 (MQ=60)

                                                                                          CCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTA      <  SRR4302535.4086857/94‑1 (MQ=60)

                                                                                           CCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAA     >  SRR4302535.1763183/1‑94 (MQ=60)

                                                                                           CCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAA     >  SRR4302535.4640851/1‑94 (MQ=60)

                                                                                            CCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAA    >  SRR4302535.935197/1‑94 (MQ=60)

                                                                                            CCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATCCTTGCCAGATAGCTAAA    >  SRR4302535.739000/1‑94 (MQ=60)

                                                                                             CCTGTGAAGTAACTCCATAAATGCCTCATATCCTTTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAT   <  SRR4302535.717558/94‑1 (MQ=60)

                                                                                             CCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAT   >  SRR4302535.2710221/1‑94 (MQ=60)

                                                                                             CCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAT   >  SRR4302535.1433242/1‑94 (MQ=60)

                                                                                              CTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATA  >  SRR4302535.421012/1‑94 (MQ=60)

                                                                                              CTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATA  >  SRR4302535.2548416/1‑94 (MQ=60)

CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATA  >  NC_000913/4085601‑4085788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`