Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,853	G→A	V178I (GTT→ATT)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,853	0	G	A	99.6%	873.2 / NA	242	V178I (GTT→ATT)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (114/127); minor base T (0/1); total (114/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       <  2:200947/93‑1 (MQ=255)

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       <  1:802879/93‑1 (MQ=255)

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       >  1:864061/1‑93 (MQ=255)

     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    >  1:4246441/1‑93 (MQ=255)

      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  1:4029125/93‑1 (MQ=255)

       aagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAg                                                                                  >  1:3662651/1‑93 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  2:3057213/1‑93 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  1:4567371/1‑93 (MQ=255)

         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                <  1:3388634/93‑1 (MQ=255)

          tGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCt                                                                               >  2:3848939/1‑93 (MQ=255)

           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              >  1:3092842/1‑93 (MQ=255)

           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              <  2:244284/93‑1 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             >  1:4099105/1‑93 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             <  2:4590957/93‑1 (MQ=255)

                aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCa                                                                         >  1:1959644/1‑93 (MQ=255)

                 caagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                        >  2:1483792/3‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:593657/93‑1 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  2:308017/1‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  1:3292490/1‑93 (MQ=255)

                   agaAATCTCGTTTGAAAGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                      <  2:1732317/93‑1 (MQ=255)

                   agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                      <  1:1171656/93‑1 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     <  2:2295177/93‑1 (MQ=255)

                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  1:2584655/1‑93 (MQ=255)

                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  1:3589814/1‑93 (MQ=255)

                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 >  1:1038584/1‑93 (MQ=255)

                            gTCTGAAATTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             <  1:3209060/93‑1 (MQ=255)

                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  2:3266412/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                            <  2:824884/93‑1 (MQ=255)

                              cggAAAGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGTTTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:1239388/91‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           >  1:4461360/1‑93 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:2942605/93‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  1:2698268/93‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:1366182/93‑1 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  1:2770189/1‑93 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  2:4208349/1‑93 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                        <  1:3315221/93‑1 (MQ=255)

                                  aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg                                                       >  2:3753711/1‑93 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                      >  2:3970239/1‑93 (MQ=255)

                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     >  2:4054161/1‑93 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  1:1130869/93‑1 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  1:3968980/93‑1 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  1:2923613/1‑93 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  1:1794878/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  2:3559836/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:3789740/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:1960451/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:1306493/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:63922/1‑93 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               >  2:2478606/1‑93 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:2456363/93‑1 (MQ=255)

                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             <  2:22192/93‑1 (MQ=255)

                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             >  1:1410586/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:521426/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  2:1020053/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            <  1:2708821/93‑1 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            <  1:3938150/93‑1 (MQ=255)

                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           >  1:906255/1‑93 (MQ=255)

                                              cTATCTACGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           <  1:934583/93‑1 (MQ=255)

                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         <  1:3471512/93‑1 (MQ=255)

                                                aTCTTCGGGCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAACCAGCGATGAAAAAAGCGGCGATTTAATCGCTGca                                         >  2:737613/1‑93 (MQ=255)

                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        >  2:1538795/1‑93 (MQ=255)

                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       >  2:1322921/1‑93 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      >  1:119085/1‑93 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  2:447931/93‑1 (MQ=255)

                                                    tCGCTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                     <  1:2067616/93‑1 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:2790174/93‑1 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  1:151008/93‑1 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:3854220/1‑93 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:2736097/1‑93 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:520172/1‑93 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 <  2:2553448/93‑1 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 <  1:3020919/93‑1 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 >  1:312662/1‑93 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  2:1513507/93‑1 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:2217991/93‑1 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  1:4633850/1‑93 (MQ=255)

                                                             cGACCCCGGTAGAGCTGGACTTCAGCCTGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  1:3085289/93‑1 (MQ=255)

                                                             cGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  1:3149562/93‑1 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:4281696/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:2820026/93‑1 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       >  2:3445112/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:4333486/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTACCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:16121/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:395831/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:1874377/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  2:161965/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  1:1218958/93‑1 (MQ=255)

                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    <  2:3756991/93‑1 (MQ=255)

                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 >  2:2781197/1‑93 (MQ=255)

                                                                         agCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                >  2:3675127/1‑93 (MQ=255)

                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              >  1:2312310/1‑93 (MQ=255)

                                                                            tGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg             <  2:2652904/93‑1 (MQ=255)

                                                                             ggACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc            >  1:1730431/1‑93 (MQ=255)

                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt        <  1:415347/93‑1 (MQ=255)

                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       >  1:1020437/1‑93 (MQ=255)

                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      >  2:167825/1‑93 (MQ=255)

                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtttt     >  1:4390807/1‑93 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   >  2:1905618/1‑93 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   <  1:1590974/93‑1 (MQ=255)

                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  <  2:1146668/93‑1 (MQ=255)

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT  >  NC_000913/4175760‑4175945

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGA                                                                                              >  SRR4302535.3674716/1‑94 (MQ=60)

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGA                                                                                              >  SRR4302535.776704/1‑94 (MQ=60)

 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                             >  SRR4302535.4073057/1‑94 (MQ=60)

  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                            <  SRR4302535.460304/94‑1 (MQ=60)

  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                            >  SRR4302535.2006320/1‑94 (MQ=60)

  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                            <  SRR4302535.3023500/94‑1 (MQ=60)

  TGGTGAAGAAGTGTATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                            <  SRR4302535.59581/94‑1 (MQ=60)

   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTG                                                                                           >  SRR4302535.2538941/1‑94 (MQ=60)

    TTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGA                                                                                          >  SRR4302535.870168/1‑94 (MQ=60)

     TGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAA                                                                                         <  SRR4302535.808520/94‑1 (MQ=60)

     TGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAA                                                                                         >  SRR4302535.933999/1‑94 (MQ=60)

     TGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAA                                                                                         <  SRR4302535.935400/94‑1 (MQ=60)

       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                       >  SRR4302535.4275515/1‑94 (MQ=60)

        AGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAA                                                                                      <  SRR4302535.283982/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                     <  SRR4302535.4056689/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                     <  SRR4302535.1286284/94‑1 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                     >  SRR4302535.3688012/1‑94 (MQ=60)

         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                     >  SRR4302535.75792/1‑94 (MQ=60)

          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGC                                                                                    >  SRR4302535.4598525/1‑94 (MQ=60)

           AGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCC                                                                                   >  SRR4302535.4026614/1‑94 (MQ=60)

            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                  <  SRR4302535.3412208/94‑1 (MQ=60)

            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                  >  SRR4302535.2416626/1‑94 (MQ=60)

             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                                 <  SRR4302535.2771624/94‑1 (MQ=60)

             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                                 >  SRR4302535.3114380/1‑94 (MQ=60)

              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                >  SRR4302535.4127170/1‑94 (MQ=60)

              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                >  SRR4302535.2770830/1‑94 (MQ=60)

              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                <  SRR4302535.330709/94‑1 (MQ=60)

               GATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAC                                                                               <  SRR4302535.4087199/94‑1 (MQ=60)

               GATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAC                                                                               >  SRR4302535.4036306/1‑94 (MQ=60)

                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                            <  SRR4302535.510861/94‑1 (MQ=60)

                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                            >  SRR4302535.1973778/1‑94 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                          >  SRR4302535.3315382/1‑94 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                          >  SRR4302535.3115875/1‑94 (MQ=60)

                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                          <  SRR4302535.3326468/94‑1 (MQ=60)

                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                         <  SRR4302535.597819/94‑1 (MQ=60)

                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                         <  SRR4302535.2206485/94‑1 (MQ=60)

                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGA                                                                        >  SRR4302535.1780776/1‑94 (MQ=60)

                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGA                                                                        <  SRR4302535.1180022/94‑1 (MQ=60)

                       GAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAT                                                                       <  SRR4302535.1528224/94‑1 (MQ=60)

                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                      >  SRR4302535.3001858/1‑94 (MQ=60)

                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                      >  SRR4302535.3614715/1‑94 (MQ=60)

                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                      >  SRR4302535.2603220/1‑94 (MQ=60)

                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                     <  SRR4302535.1934942/94‑1 (MQ=60)

                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                    >  SRR4302535.1045957/1‑94 (MQ=60)

                             TCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAA                                                                 <  SRR4302535.415569/94‑1 (MQ=60)

                               GTCTGAAATTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAG                                                               <  SRR4302535.3231289/94‑1 (MQ=60)

                               GTCTGAAAGTGTCTGTTTCTATCTTCGGTCTTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGCTCAAAAAAC                                                               >  SRR4302535.543852/1‑94 (MQ=60)

                               GTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAG                                                               >  SRR4302535.3966288/1‑94 (MQ=60)

                               GTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAG                                                               >  SRR4302535.1829061/1‑94 (MQ=60)

                                TCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                              >  SRR4302535.4491959/1‑94 (MQ=60)

                                TCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                              <  SRR4302535.3182251/94‑1 (MQ=60)

                                TCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                              <  SRR4302535.79886/94‑1 (MQ=60)

                                TCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGC                                                              <  SRR4302535.1216298/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                             <  SRR4302535.231025/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                             <  SRR4302535.2717551/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                             <  SRR4302535.1354388/94‑1 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                             >  SRR4302535.2789917/1‑94 (MQ=60)

                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                             <  SRR4302535.4520220/94‑1 (MQ=60)

                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                          <  SRR4302535.3338287/94‑1 (MQ=60)

                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                        >  SRR4302535.3994327/1‑94 (MQ=60)

                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                      <  SRR4302535.2724122/94‑1 (MQ=60)

                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                      <  SRR4302535.1138941/94‑1 (MQ=60)

                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                      <  SRR4302535.3996088/94‑1 (MQ=60)

                                         GTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTA                                                     >  SRR4302535.2944230/1‑94 (MQ=60)

                                         GTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTA                                                     >  SRR4302535.1807852/1‑94 (MQ=60)

                                          TCTGTTTCTATCTTCGGTCGTGCGACCCCGGCAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAACGCGGCGATTTAA                                                    >  SRR4302535.3153915/1‑94 (MQ=60)

                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCGTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                   >  SRR4302535.2645611/1‑94 (MQ=60)

                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                   <  SRR4302535.899927/94‑1 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                  <  SRR4302535.1315823/94‑1 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                  <  SRR4302535.3815876/94‑1 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                  <  SRR4302535.348196/94‑1 (MQ=60)

                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                  <  SRR4302535.1739488/94‑1 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                 <  SRR4302535.3145315/94‑1 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                 >  SRR4302535.3110755/1‑94 (MQ=60)

                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                 <  SRR4302535.2474077/94‑1 (MQ=60)

                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                                <  SRR4302535.1915777/94‑1 (MQ=60)

                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                                >  SRR4302535.1420658/1‑94 (MQ=60)

                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                               >  SRR4302535.525074/1‑94 (MQ=60)

                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                               <  SRR4302535.852175/94‑1 (MQ=60)

                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                               >  SRR4302535.1606907/1‑94 (MQ=60)

                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                              <  SRR4302535.3965026/94‑1 (MQ=60)

                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                              >  SRR4302535.912687/1‑94 (MQ=60)

                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                              <  SRR4302535.2728181/94‑1 (MQ=60)

                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                             >  SRR4302535.4418361/1‑94 (MQ=60)

                                                 CTATCTACGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                             <  SRR4302535.941201/94‑1 (MQ=60)

                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                            >  SRR4302535.1633099/1‑94 (MQ=60)

                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                            <  SRR4302535.4293651/94‑1 (MQ=60)

                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                            >  SRR4302535.1496361/1‑94 (MQ=60)

                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                            >  SRR4302535.3939336/1‑94 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAC                                           >  SRR4302535.4478922/1‑94 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAC                                           <  SRR4302535.3495634/94‑1 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAC                                           >  SRR4302535.1340189/1‑94 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAC                                           >  SRR4302535.3085410/1‑94 (MQ=60)

                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAA                                           >  SRR4302535.3445118/1‑94 (MQ=60)

                                                    TCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACA                                          >  SRR4302535.1158652/1‑94 (MQ=60)

                                                     CTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                         >  SRR4302535.3629602/1‑94 (MQ=60)

                                                     CTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                         >  SRR4302535.119917/1‑94 (MQ=60)

                                                     CTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                         >  SRR4302535.2615211/1‑94 (MQ=60)

                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                        <  SRR4302535.524911/94‑1 (MQ=60)

                                                       TCGCTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                       <  SRR4302535.2082519/94‑1 (MQ=60)

                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                     <  SRR4302535.152066/94‑1 (MQ=60)

                                                          GTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGT                                    >  SRR4302535.314828/1‑94 (MQ=60)

                                                           TCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTG                                   <  SRR4302535.3042076/94‑1 (MQ=60)

                                                           TCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTG                                   <  SRR4302535.1194999/94‑1 (MQ=60)

                                                           GCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTG                                   <  SRR4302535.1147373/94‑1 (MQ=60)

                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                 >  SRR4302535.3957128/1‑94 (MQ=60)

                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                 >  SRR4302535.4665283/1‑94 (MQ=60)

                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                 <  SRR4302535.2233774/94‑1 (MQ=60)

                                                              TGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGA                                >  SRR4302535.369430/1‑94 (MQ=60)

                                                               GCGACACCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAT                               <  SRR4302535.1584933/94‑1 (MQ=60)

                                                                CGACCCCGGTAGAGCTGGACTTCAGCCTGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                              <  SRR4302535.3106782/94‑1 (MQ=60)

                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                              <  SRR4302535.3180787/94‑1 (MQ=60)

                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                              <  SRR4302535.3171455/94‑1 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTACCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                        <  SRR4302535.16241/94‑1 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGTCGTGAGATTGGAATA                        >  SRR4302535.4635969/1‑94 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                        <  SRR4302535.1887880/94‑1 (MQ=60)

                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                        <  SRR4302535.398604/94‑1 (MQ=60)

                                                                       GGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAGAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAC                       <  SRR4302535.3318837/94‑1 (MQ=60)

                                                                       GGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAC                       <  SRR4302535.1227701/94‑1 (MQ=60)

                                                                        GTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACA                      <  SRR4302535.4506914/94‑1 (MQ=60)

                                                                         TAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAA                     <  SRR4302535.1119361/94‑1 (MQ=60)

                                                                         TAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAA                     <  SRR4302535.2181403/94‑1 (MQ=60)

                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                    >  SRR4302535.1208383/1‑94 (MQ=60)

                                                                           GAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATT                   <  SRR4302535.103074/94‑1 (MQ=60)

                                                                            AGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTT                  <  SRR4302535.2329015/94‑1 (MQ=60)

                                                                             GCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTC                 >  SRR4302535.2956128/1‑94 (MQ=60)

                                                                             GCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTC                 >  SRR4302535.2328863/1‑94 (MQ=60)

                                                                              CTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCG                >  SRR4302535.3549262/1‑94 (MQ=60)

                                                                               TGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGC               >  SRR4302535.1742942/1‑94 (MQ=60)

                                                                                 GACTTCAGCCAGATTGAAAAAGCCTAACCCACCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGGGTGAGATTGGAATACAATTTgccgc             >  SRR4302535.1595480/1‑89 (MQ=60)

                                                                                    TTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTT          >  SRR4302535.1027689/1‑94 (MQ=60)

                                                                                    TTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTT          <  SRR4302535.4138825/94‑1 (MQ=60)

                                                                                    TTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTT          <  SRR4302535.418259/94‑1 (MQ=60)

                                                                                      CAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTT        <  SRR4302535.4200890/94‑1 (MQ=60)

                                                                                      CAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTT        >  SRR4302535.4420851/1‑94 (MQ=60)

                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT     <  SRR4302535.1602453/94‑1 (MQ=60)

                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT     <  SRR4302535.3649101/94‑1 (MQ=60)

                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT     <  SRR4302535.3373559/94‑1 (MQ=60)

                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT    >  SRR4302535.3281545/1‑94 (MQ=60)

                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT   >  SRR4302535.2569646/1‑94 (MQ=60)

                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT   >  SRR4302535.786103/1‑94 (MQ=60)

                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT   >  SRR4302535.3845715/1‑94 (MQ=60)

                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT  <  SRR4302535.2323683/94‑1 (MQ=60)

                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT  <  SRR4302535.3401091/94‑1 (MQ=60)

                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT  >  SRR4302535.1658289/1‑94 (MQ=60)

GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT  >  NC_000913/4175760‑4175945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`