Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,201,958 G→A W354* (TGG→TAG)  hydG → HydG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,201,9580GA100.0% 823.5 / NA 225W354* (TGG→TAG) hydGHydG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (116/109);  total (116/109)

Only 100 of 231 total aligned reads displayed.

GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC  >  NC_000913/4201868‑4202047
                                                                                          |                                                                                         
gcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGc                                                                                         >  1:4475376/1‑93 (MQ=255)
gcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGc                                                                                         >  1:563054/1‑93 (MQ=255)
 ccATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGcc                                                                                        <  1:2540657/93‑1 (MQ=255)
 ccATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGcc                                                                                        <  2:2296051/93‑1 (MQ=255)
 ccATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGcc                                                                                        >  2:1141730/1‑93 (MQ=255)
   aTTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGcccg                                                                                      >  1:4250879/1‑91 (MQ=255)
   aTTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCgg                                                                                      >  2:1027302/1‑93 (MQ=255)
   aTTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCgg                                                                                      >  1:3500990/1‑93 (MQ=255)
   aTTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCgg                                                                                      <  2:71903/93‑1 (MQ=255)
   aTTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCgg                                                                                      <  1:1004700/93‑1 (MQ=255)
    ttttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCggg                                                                                     >  1:1412390/1‑93 (MQ=255)
    ttttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCggg                                                                                     >  1:4591849/1‑93 (MQ=255)
    ttttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCggg                                                                                     <  2:3859424/93‑1 (MQ=255)
    ttttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCggg                                                                                     >  2:2994012/1‑93 (MQ=255)
    ttttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCggg                                                                                     >  2:4405543/1‑93 (MQ=255)
      ttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaa                                                                                   <  2:3500355/93‑1 (MQ=255)
       tCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaaa                                                                                  <  2:4193142/93‑1 (MQ=255)
         tGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGACCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAata                                                                                <  1:2376347/93‑1 (MQ=255)
          gCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAatat                                                                               >  2:356836/1‑93 (MQ=255)
          gCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAatat                                                                               >  1:2299099/1‑93 (MQ=255)
            aGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTc                                                                             <  1:834322/93‑1 (MQ=255)
             gcgcTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCg                                                                            <  2:3117152/93‑1 (MQ=255)
                cTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGa                                                                         <  1:1791301/93‑1 (MQ=255)
                cTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGa                                                                         >  1:1051459/1‑93 (MQ=255)
                cTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGa                                                                         <  2:1574099/93‑1 (MQ=255)
                   tGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCt                                                                      <  2:3452141/93‑1 (MQ=255)
                   tGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCt                                                                      >  1:969253/1‑93 (MQ=255)
                       gAGCGTAATCAGAAGGCGGTAAAAGGGTTTACGCTCCAGACGATGAATCTGTTGATTCATTACGTCTACCCGGGAAATATTCGTGAGCCGGaa                                                                  <  2:626816/93‑1 (MQ=255)
                        aGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaaa                                                                 <  1:2923241/93‑1 (MQ=255)
                        aGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaaa                                                                 >  1:695889/1‑93 (MQ=255)
                         gCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaaaa                                                                <  1:3397544/93‑1 (MQ=255)
                         gCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaaaa                                                                <  2:467774/93‑1 (MQ=255)
                          cGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAc                                                               <  1:160298/93‑1 (MQ=255)
                          cGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAc                                                               <  2:2733928/93‑1 (MQ=255)
                           gTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAcg                                                              >  2:3955239/1‑93 (MQ=255)
                             aaTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAcgcg                                                            <  1:2624095/93‑1 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           <  2:3423473/93‑1 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           >  2:1323273/1‑93 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           >  2:4654151/1‑93 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           <  2:2369929/93‑1 (MQ=255)
                               tCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCggg                                                          >  2:1456245/1‑92 (MQ=255)
                                 gCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTgg                                                        <  1:352733/93‑1 (MQ=255)
                                 gCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTgg                                                        >  2:2473226/1‑93 (MQ=255)
                                   aaGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGaa                                                      >  2:3285440/1‑93 (MQ=255)
                                   aaGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGaa                                                      >  1:3906204/1‑93 (MQ=255)
                                   aaGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGaa                                                      <  2:855869/93‑1 (MQ=255)
                                    aGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAc                                                     <  1:2655362/93‑1 (MQ=255)
                                        ggTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGc                                                 >  1:4321435/1‑93 (MQ=255)
                                         gaaaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCa                                                >  2:2354971/3‑93 (MQ=255)
                                         gTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCa                                                <  2:2544354/93‑1 (MQ=255)
                                          tAAAAGTTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAg                                               <  1:231957/93‑1 (MQ=255)
                                           aaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgt                                              >  1:953215/1‑93 (MQ=255)
                                            aaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTAGTGAGCTTGAAAACGCGGTGGTACGGGCAgtg                                             >  2:2110730/1‑93 (MQ=255)
                                             aaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtgg                                            <  2:1127738/93‑1 (MQ=255)
                                              aGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggt                                           <  2:907524/93‑1 (MQ=255)
                                              aGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggt                                           <  1:4165213/93‑1 (MQ=255)
                                               ggTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggtg                                          >  1:3216927/1‑93 (MQ=255)
                                                gTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgc                                         <  2:2800731/93‑1 (MQ=255)
                                                gTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgc                                         <  1:3631357/93‑1 (MQ=255)
                                                 ttttACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgct                                        >  1:2061153/1‑93 (MQ=255)
                                                 ttttACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgct                                        <  1:245777/93‑1 (MQ=255)
                                                 ttttACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgct                                        >  1:1150745/1‑93 (MQ=255)
                                                  tttACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgctg                                       >  2:1162694/1‑93 (MQ=255)
                                                     aCGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGctgctg                                    <  1:2849105/93‑1 (MQ=255)
                                                       gCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAc                                  >  1:60959/1‑93 (MQ=255)
                                                         cccAGGCGATGGATCTGTTGATGCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGACCGGGCAGTGGTGCTGCTGACCg                                <  2:3685786/93‑1 (MQ=255)
                                                          ccAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgg                               <  2:1667658/93‑1 (MQ=255)
                                                          ccAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgg                               >  2:363502/1‑93 (MQ=255)
                                                           cAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                              <  2:1490284/93‑1 (MQ=255)
                                                           cAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                              >  2:4112042/1‑93 (MQ=255)
                                                           cAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                              >  1:540263/1‑93 (MQ=255)
                                                           cAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                              >  2:4286028/1‑93 (MQ=255)
                                                             ggCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGa                            >  1:2131429/1‑93 (MQ=255)
                                                              gCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGaa                           <  1:3180552/93‑1 (MQ=255)
                                                                gATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAata                         >  2:2584190/1‑93 (MQ=255)
                                                                gATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAata                         >  2:2893137/1‑93 (MQ=255)
                                                                gATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAata                         >  1:474825/1‑93 (MQ=255)
                                                                 aTGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatat                        >  1:439078/1‑93 (MQ=255)
                                                                  tGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatata                       >  1:102961/1‑93 (MQ=255)
                                                                   ggATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatatat                      >  2:395003/1‑93 (MQ=255)
                                                                   ggATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatatat                      <  1:2392977/93‑1 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    >  2:4172517/1‑93 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   <  1:4522708/93‑1 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   <  1:3958283/93‑1 (MQ=255)
                                                                       cTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTcc                  >  2:2138620/1‑93 (MQ=255)
                                                                         gTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGa                <  1:1116656/93‑1 (MQ=255)
                                                                         gTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGa                >  2:1869290/1‑93 (MQ=255)
                                                                          ttGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa               <  2:1349339/93‑1 (MQ=255)
                                                                          ttGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa               >  2:4127724/1‑93 (MQ=255)
                                                                          ttGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa               <  1:2958155/93‑1 (MQ=255)
                                                                           tGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAc              >  2:2167770/1‑93 (MQ=255)
                                                                           tGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAc              <  2:4475376/93‑1 (MQ=255)
                                                                              ttCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgcg           <  2:4056062/93‑1 (MQ=255)
                                                                               tCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGa          <  1:3175229/93‑1 (MQ=255)
                                                                                 aTTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGc        >  2:956442/1‑93 (MQ=255)
                                                                                    aCGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGc     <  1:732023/93‑1 (MQ=255)
                                                                                    aCGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGc     <  2:814300/93‑1 (MQ=255)
                                                                                      gACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCg   >  1:1729433/1‑93 (MQ=255)
                                                                                      gACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCg   >  2:26954/1‑93 (MQ=255)
                                                                                       aCTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGc  <  1:1226798/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC  >  NC_000913/4201868‑4202047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGG  >  NC_000913/4201865‑4202050
                                                                                             |                                                                                            
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTA                                                                                              >  SRR4302535.2216658/1‑94 (MQ=60)
 TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAG                                                                                             <  SRR4302535.3774319/94‑1 (MQ=60)
 TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAG                                                                                             <  SRR4302535.2410798/94‑1 (MQ=60)
 TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAG                                                                                             >  SRR4302535.93417/1‑94 (MQ=60)
  GGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGC                                                                                            >  SRR4302535.566996/1‑94 (MQ=60)
  GGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGC                                                                                            >  SRR4302535.4506084/1‑94 (MQ=60)
  GGCCATTTGCTGCAGCGCTTTTCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGC                                                                                            <  SRR4302535.688970/94‑1 (MQ=60)
   GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCC                                                                                           >  SRR4302535.1024060/1‑94 (MQ=60)
    CCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCG                                                                                          <  SRR4302535.2558957/94‑1 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                         >  SRR4302535.3525330/1‑94 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                         >  SRR4302535.2784685/1‑94 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                         <  SRR4302535.2668204/94‑1 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                         >  SRR4302535.2356397/1‑94 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCCG                                                                                         >  SRR4302535.4279996/1‑94 (MQ=60)
      ATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGG                                                                                        >  SRR4302535.4623108/1‑94 (MQ=60)
      ATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGG                                                                                        >  SRR4302535.1422473/1‑94 (MQ=60)
      ATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGG                                                                                        <  SRR4302535.1011837/94‑1 (MQ=60)
       TTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGA                                                                                       >  SRR4302535.991707/1‑94 (MQ=60)
           CTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATA                                                                                   >  SRR4302535.1876736/1‑94 (MQ=60)
            TGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAT                                                                                  <  SRR4302535.1680730/94‑1 (MQ=60)
            TGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAT                                                                                  >  SRR4302535.2547861/1‑94 (MQ=60)
            TGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAT                                                                                  >  SRR4302535.2315547/1‑94 (MQ=60)
            TGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGACCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAT                                                                                  <  SRR4302535.2393417/94‑1 (MQ=60)
             GCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATT                                                                                 <  SRR4302535.4378729/94‑1 (MQ=60)
              CAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTC                                                                                <  SRR4302535.2568070/94‑1 (MQ=60)
               AGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGGTCTGTTGATTCATTACGACTAGCCGGGAAATATTCG                                                                               >  SRR4302535.3197523/1‑94 (MQ=60)
               AGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCG                                                                               >  SRR4302535.4579681/1‑94 (MQ=60)
               AGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCG                                                                               <  SRR4302535.840172/94‑1 (MQ=60)
                 CGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTG                                                                             >  SRR4302535.4671986/1‑94 (MQ=60)
                  GCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGA                                                                            >  SRR4302535.1058937/1‑94 (MQ=60)
                   CTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAG                                                                           <  SRR4302535.1804254/94‑1 (MQ=60)
                     TTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCT                                                                         >  SRR4302535.976137/1‑94 (MQ=60)
                          GAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAA                                                                    >  SRR4302535.700770/1‑94 (MQ=60)
                           AGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAA                                                                   <  SRR4302535.2943855/94‑1 (MQ=60)
                           AGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAA                                                                   >  SRR4302535.581443/1‑94 (MQ=60)
                            GCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAC                                                                  <  SRR4302535.2959806/94‑1 (MQ=60)
                            GCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAC                                                                  <  SRR4302535.3421185/94‑1 (MQ=60)
                             CGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACG                                                                 <  SRR4302535.161422/94‑1 (MQ=60)
                               TAATCGCAAGGCGGTAAAAGGTTTTACCCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCG                                                               <  SRR4302535.3585434/94‑1 (MQ=60)
                                AATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGG                                                              <  SRR4302535.2642902/94‑1 (MQ=60)
                                 ATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGT                                                             <  SRR4302535.4511922/94‑1 (MQ=60)
                                  TCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTG                                                            <  SRR4302535.927071/94‑1 (MQ=60)
                                  TCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTG                                                            <  SRR4302535.4234439/94‑1 (MQ=60)
                                   CGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGGAAACGCGGTGG                                                           <  SRR4302535.2548405/94‑1 (MQ=60)
                                   CGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGG                                                           >  SRR4302535.1548417/1‑94 (MQ=60)
                                   CGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGG                                                           >  SRR4302535.1130293/1‑94 (MQ=60)
                                    GCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGA                                                          <  SRR4302535.355166/94‑1 (MQ=60)
                                     CAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAA                                                         >  SRR4302535.3932966/1‑94 (MQ=60)
                                      AAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAC                                                        >  SRR4302535.4346795/1‑94 (MQ=60)
                                       AGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACG                                                       <  SRR4302535.2674388/94‑1 (MQ=60)
                                        GGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGG                                                      >  SRR4302535.2426368/1‑94 (MQ=60)
                                         GCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGG                                                     <  SRR4302535.2143365/94‑1 (MQ=60)
                                         GCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGG                                                     <  SRR4302535.2835526/94‑1 (MQ=60)
                                          CGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGC                                                    >  SRR4302535.4350995/1‑94 (MQ=60)
                                            GTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAG                                                  >  SRR4302535.345146/1‑94 (MQ=60)
                                             TAAAAGTTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGT                                                 <  SRR4302535.233558/94‑1 (MQ=60)
                                             TAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGT                                                 >  SRR4302535.959970/1‑94 (MQ=60)
                                              AAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTG                                                >  SRR4302535.2935490/1‑94 (MQ=60)
                                                AAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGT                                              >  SRR4302535.678933/1‑94 (MQ=60)
                                                 AGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTG                                             <  SRR4302535.4193734/94‑1 (MQ=60)
                                                 AGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTG                                             >  SRR4302535.3239210/1‑94 (MQ=60)
                                                  GGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGC                                            >  SRR4302535.707949/1‑94 (MQ=60)
                                                   GTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCT                                           >  SRR4302535.2076006/1‑94 (MQ=60)
                                                   GTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCT                                           <  SRR4302535.3656531/94‑1 (MQ=60)
                                                   GTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCT                                           >  SRR4302535.1158964/1‑94 (MQ=60)
                                                   GTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCT                                           <  SRR4302535.3532795/94‑1 (MQ=60)
                                                    TTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTG                                          <  SRR4302535.247459/94‑1 (MQ=60)
                                                    TTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTG                                          <  SRR4302535.3713756/94‑1 (MQ=60)
                                                    TTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTG                                          >  SRR4302535.397331/1‑94 (MQ=60)
                                                      TTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCT                                        >  SRR4302535.1054047/1‑94 (MQ=60)
                                                        ACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGA                                      <  SRR4302535.2869294/94‑1 (MQ=60)
                                                         CGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAC                                     >  SRR4302535.61414/1‑94 (MQ=60)
                                                          GCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACC                                    >  SRR4302535.2320875/1‑94 (MQ=60)
                                                           CCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCG                                   >  SRR4302535.3395084/1‑94 (MQ=60)
                                                            CCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGG                                  >  SRR4302535.2403314/1‑94 (MQ=60)
                                                             CCAGGCGATGGATCTGTTGATTCATTCAGTCTAGCCGGGAAAGATTCGTGAGCGGGAAAATGCGGGGGAACGGGCAGTGGggttgttgcccggg                                 >  SRR4302535.3233590/1‑80 (MQ=39)
                                                             CCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                 >  SRR4302535.1793097/1‑94 (MQ=60)
                                                             CCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                 >  SRR4302535.544032/1‑94 (MQ=60)
                                                               AGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGA                               >  SRR4302535.2146794/1‑94 (MQ=60)
                                                                GGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAA                              >  SRR4302535.1088118/1‑94 (MQ=60)
                                                                 GCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAAT                             <  SRR4302535.3202538/94‑1 (MQ=60)
                                                                  CGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATA                            >  SRR4302535.478153/1‑94 (MQ=60)
                                                                   GATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATAT                           <  SRR4302535.57615/94‑1 (MQ=60)
                                                                   GATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATAT                           >  SRR4302535.4709879/1‑94 (MQ=60)
                                                                   GATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATAT                           >  SRR4302535.442152/1‑94 (MQ=60)
                                                                   GATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATAT                           <  SRR4302535.31232/94‑1 (MQ=60)
                                                                   GATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGAGCAGTGGTGCTGCTGACCCGGGAATAT                           >  SRR4302535.4472433/1‑94 (MQ=60)
                                                                    ATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATA                          >  SRR4302535.103690/1‑94 (MQ=60)
                                                                      GGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATT                        <  SRR4302535.2410177/94‑1 (MQ=60)
                                                                      GGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATT                        >  SRR4302535.1193598/1‑94 (MQ=60)
                                                                       GATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTT                       >  SRR4302535.835647/1‑94 (MQ=60)
                                                                         TCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCC                     >  SRR4302535.1832177/1‑94 (MQ=60)
                                                                         TCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCC                     <  SRR4302535.4553698/94‑1 (MQ=60)
                                                                         TCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCC                     <  SRR4302535.3985314/94‑1 (MQ=60)
                                                                          CTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCG                    >  SRR4302535.1419403/1‑94 (MQ=60)
                                                                          CTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCG                    <  SRR4302535.4019287/94‑1 (MQ=60)
                                                                            GTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAA                  <  SRR4302535.1124619/94‑1 (MQ=60)
                                                                            GTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAA                  >  SRR4302535.895544/1‑94 (MQ=60)
                                                                             TTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAC                 <  SRR4302535.965939/94‑1 (MQ=60)
                                                                             TTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAC                 <  SRR4302535.2978929/94‑1 (MQ=60)
                                                                             TTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAC                 <  SRR4302535.3115543/94‑1 (MQ=60)
                                                                               GATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGC               >  SRR4302535.546942/1‑94 (MQ=60)
                                                                               GATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGC               >  SRR4302535.3221040/1‑94 (MQ=60)
                                                                                ATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATGTTTCCGAACGCG              <  SRR4302535.505052/94‑1 (MQ=60)
                                                                                  TCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAG            <  SRR4302535.3197202/94‑1 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCT          <  SRR4302535.2321564/94‑1 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCT          <  SRR4302535.4264123/94‑1 (MQ=60)
                                                                                      TACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGC        <  SRR4302535.2171824/94‑1 (MQ=60)
                                                                                       ACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCC       <  SRR4302535.329880/94‑1 (MQ=60)
                                                                                       ACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCC       <  SRR4302535.737161/94‑1 (MQ=60)
                                                                                        CGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCG      >  SRR4302535.1741932/1‑94 (MQ=60)
                                                                                        CGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCG      >  SRR4302535.3817469/1‑94 (MQ=60)
                                                                                        CGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCG      <  SRR4302535.4442123/94‑1 (MQ=60)
                                                                                        CGACTAGCCGGGAAATATTCGTGAGCTGGAAAACACGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCG      >  SRR4302535.1131483/1‑94 (MQ=60)
                                                                                         GACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC     >  SRR4302535.152789/1‑94 (MQ=60)
                                                                                          ACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCT    <  SRR4302535.1235572/94‑1 (MQ=60)
                                                                                          ACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCT    >  SRR4302535.4191708/1‑94 (MQ=60)
                                                                                           CTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTG   >  SRR4302535.3736753/1‑94 (MQ=60)
                                                                                           CTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTG   >  SRR4302535.3773504/1‑94 (MQ=60)
                                                                                            TAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGG  >  SRR4302535.1303303/1‑94 (MQ=60)
                                                                                             |                                                                                            
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGG  >  NC_000913/4201865‑4202050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑