Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,205,438 T→C intergenic (‑328/‑287) purH ← / → rrsE phosphoribosylaminoimidazolecarboxamideformyltra nsferase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,205,4380TC100.0% 542.2 / NA 176intergenic (‑328/‑287)purH/rrsEphosphoribosylaminoimidazolecarboxamideformyltra nsferase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (104/72);  total (104/72)

Only 100 of 192 total aligned reads displayed.

TAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG  >  NC_000913/4205348‑4205512
                                                                                          |                                                                          
taAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCcccc                                                                          >  2:49345/1‑93 (MQ=255)
taAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCcccc                                                                          >  2:4374671/1‑93 (MQ=255)
 aaaGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCccccc                                                                         >  2:2031736/1‑92 (MQ=255)
  aaGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGCGAACTCCCTATAATGCGCCCCCAt                                                                        >  1:936504/1‑93 (MQ=255)
    gCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCg                                                                      >  2:4619197/1‑93 (MQ=255)
     cGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGa                                                                     <  2:4266595/93‑1 (MQ=255)
      gggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGac                                                                    >  2:2667448/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATTATGCGCCCCCATCGaca                                                                   >  1:3342481/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGaca                                                                   >  1:1001070/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGaca                                                                   >  2:2275760/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGaca                                                                   >  2:371366/1‑93 (MQ=255)
       ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGaca                                                                   >  2:546079/1‑93 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  <  2:2815339/93‑1 (MQ=255)
        gCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGacac                                                                  <  1:3223004/93‑1 (MQ=255)
         cTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGTGGCCTGCGGAGTACTCCCTATAATGCGCCCCCATCGACAcg                                                                 >  1:275403/1‑93 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGCCAcgg                                                                >  1:1112997/1‑93 (MQ=255)
          ttttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcgg                                                                >  1:3326077/1‑93 (MQ=255)
           tttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggc                                                               <  1:13132/93‑1 (MQ=255)
            ttGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggcg                                                              >  2:2930708/1‑93 (MQ=255)
              gCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAcggcggg                                                            >  2:102101/1‑92 (MQ=255)
              gCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGa                                                            >  1:4176713/1‑93 (MQ=255)
                tGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtg                                                          >  1:4009103/1‑93 (MQ=255)
                tGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtg                                                          >  1:895711/1‑93 (MQ=255)
                tGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGAtg                                                          >  2:2885362/1‑93 (MQ=255)
                    gAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGaa                                                      <  1:359238/93‑1 (MQ=255)
                      aTGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATc                                                    <  1:12164/93‑1 (MQ=255)
                       tGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAAtta                                                   >  2:2481047/1‑91 (MQ=255)
                         cagcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACt                                                 <  2:184203/93‑1 (MQ=255)
                           gcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTc                                               <  2:1584733/93‑1 (MQ=255)
                           gcagTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTc                                               >  1:2010606/1‑93 (MQ=255)
                           acagtcagtCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTc                                               <  1:3670819/92‑1 (MQ=255)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcac                                             >  2:2527035/1‑93 (MQ=255)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcac                                             >  1:2409789/1‑93 (MQ=255)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcac                                             <  2:2973890/93‑1 (MQ=255)
                             agtcagtcATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcac                                             >  1:4163207/1‑93 (MQ=255)
                              gtcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGGACTCCCTATAATGCGCCCCCCTCCACACCGCGGGTGTGGATGAGTTcaca                                            >  2:4081363/1‑93 (MQ=25)
                              gtcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATTTGAATCACTTcaca                                            >  1:1048458/1‑93 (MQ=255)
                               tcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcacac                                           <  2:1478489/93‑1 (MQ=255)
                                cagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTcacaca                                          >  2:3884643/1‑93 (MQ=255)
                                 agtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaa                                         >  1:706838/1‑93 (MQ=255)
                                 agtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaa                                         >  2:4639464/1‑93 (MQ=255)
                                  gtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACaaa                                        >  2:2725889/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACCAAc                                       >  1:3960183/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       >  2:3191700/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       >  1:860873/1‑93 (MQ=255)
                                   tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAc                                       <  1:3913218/93‑1 (MQ=255)
                                      tttttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGc                                    <  1:194756/93‑1 (MQ=255)
                                       ttttttGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGcc                                   <  2:1639729/93‑1 (MQ=255)
                                       ttttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGcc                                   <  2:2628385/93‑1 (MQ=255)
                                        tttCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGGGAATCACTTCACACAAACAGCcg                                  >  1:3752775/1‑93 (MQ=255)
                                          tCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggt                                >  1:2429438/1‑93 (MQ=255)
                                           cTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggtt                               >  1:731943/1‑93 (MQ=255)
                                            tGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttc                              <  1:2379796/93‑1 (MQ=255)
                                            tGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttc                              >  1:3166883/1‑93 (MQ=255)
                                            tGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttc                              <  1:3773749/93‑1 (MQ=255)
                                             gCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcg                             >  1:653737/1‑93 (MQ=255)
                                             gCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcg                             <  1:2897669/93‑1 (MQ=255)
                                              cAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcgg                            <  2:2071843/93‑1 (MQ=255)
                                               aaTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcggt                           >  2:1323746/1‑93 (MQ=255)
                                                aTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcggtt                          <  2:269028/93‑1 (MQ=255)
                                                aTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcggtt                          <  1:2098546/93‑1 (MQ=255)
                                                aTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCcggttcggtt                          <  2:119325/93‑1 (MQ=255)
                                                aTTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATAACTTCACACAAACAGCcggttcggtt                          >  1:3617770/1‑93 (MQ=255)
                                                 tttttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTg                         >  2:1663292/1‑93 (MQ=255)
                                                  ttttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGa                        >  1:1793449/1‑93 (MQ=255)
                                                  ttttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGa                        >  1:3095704/1‑93 (MQ=255)
                                                   tttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGaa                       >  2:944714/1‑93 (MQ=255)
                                                   tttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGaa                       >  1:4518423/1‑93 (MQ=255)
                                                    ttCTATTGCGGCCTGCGGAGAGCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAag                      <  1:4231034/93‑1 (MQ=255)
                                                    ttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAag                      >  2:1755229/1‑93 (MQ=255)
                                                    ttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAag                      >  2:1679110/1‑93 (MQ=255)
                                                     tCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAaga                     <  2:4180406/93‑1 (MQ=255)
                                                      cTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAagag                    >  2:337356/1‑93 (MQ=255)
                                                       tATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAgaga                   >  1:4628619/1‑93 (MQ=255)
                                                       tATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAgaga                   >  1:3632421/1‑93 (MQ=255)
                                                       tATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAgaga                   >  2:1454355/1‑93 (MQ=255)
                                                        aTTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaa                  >  1:1271659/1‑93 (MQ=255)
                                                        aTTGCAGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaa                  >  2:3055186/1‑93 (MQ=255)
                                                         ttGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaaa                 <  2:3115090/93‑1 (MQ=255)
                                                          tGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGaaaa                <  2:409067/93‑1 (MQ=255)
                                                            cGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAAt              <  1:528605/93‑1 (MQ=255)
                                                             ggCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACCAACAGCCGGTTCGGTTGCAGAGAAAACTc             >  2:94710/1‑93 (MQ=14)
                                                              gCCTGCGGAGATCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAATCAGCCGGTTCGGTTGAAGAGAAAAATcc            <  1:4607114/93‑1 (MQ=255)
                                                              gCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATcc            >  1:1339068/1‑93 (MQ=255)
                                                                cTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTg          >  2:2212611/1‑93 (MQ=255)
                                                                cTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGATCGGTTGAAGAGAAAAATCCTg          >  2:2763040/1‑93 (MQ=255)
                                                                 tGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATATGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGa         >  1:325250/1‑93 (MQ=255)
                                                                  gCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGaa        <  2:2684564/93‑1 (MQ=255)
                                                                   cGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGaaa       <  1:2043574/93‑1 (MQ=37)
                                                                     gagaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAtt     <  1:1169549/93‑1 (MQ=21)
                                                                      agaCCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACCCAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    <  2:1353389/93‑1 (MQ=255)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    >  2:423630/1‑93 (MQ=18)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    >  2:1956147/1‑93 (MQ=18)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    >  1:3497713/1‑93 (MQ=18)
                                                                      agaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTc    <  2:864909/93‑1 (MQ=18)
                                                                       gaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCa   >  1:215056/1‑93 (MQ=17)
                                                                       gaACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCa   <  2:139882/93‑1 (MQ=17)
                                                                        aaCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAg  >  1:2503266/1‑93 (MQ=14)
                                                                        aaCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAg  <  1:1709240/93‑1 (MQ=14)
                                                                        aaCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAg  >  1:767850/1‑93 (MQ=14)
                                                                                          |                                                                          
TAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG  >  NC_000913/4205348‑4205512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  NC_000913/4205346‑4205531
                                                                                            |                                                                                             
TATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCC                                                                                              >  SRR4302535.1878371/1‑94 (MQ=60)
 ATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCC                                                                                             >  SRR4302535.3191428/1‑94 (MQ=60)
 ATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCC                                                                                             >  SRR4302535.3154093/1‑94 (MQ=60)
   AAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGCGAACTCCCTATAATGCGCCCCCAT                                                                                           >  SRR4302535.943137/1‑94 (MQ=60)
   AAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCAT                                                                                           <  SRR4302535.3262350/94‑1 (MQ=60)
        GGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATTATGCGCCCCCATCGACA                                                                                      >  SRR4302535.3365773/1‑94 (MQ=60)
        GGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACA                                                                                      >  SRR4302535.1008184/1‑94 (MQ=60)
         GGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACAC                                                                                     >  SRR4302535.727378/1‑94 (MQ=60)
          GCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGTGGCCTGCGGAGTACTCCCTATAATGCGCCCCCATCGACACG                                                                                    >  SRR4302535.277323/1‑94 (MQ=60)
          GCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACG                                                                                    <  SRR4302535.3245347/94‑1 (MQ=60)
           CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGCCACGG                                                                                   >  SRR4302535.1120935/1‑94 (MQ=60)
           CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGG                                                                                   >  SRR4302535.3349222/1‑94 (MQ=60)
           CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGG                                                                                   >  SRR4302535.378975/1‑94 (MQ=60)
           CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGG                                                                                   >  SRR4302535.3749625/1‑94 (MQ=60)
           CTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGG                                                                                   <  SRR4302535.3046145/94‑1 (MQ=60)
             TTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCG                                                                                 <  SRR4302535.13231/94‑1 (MQ=60)
               TGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGA                                                                               >  SRR4302535.4205308/1‑94 (MQ=60)
                 CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             >  SRR4302535.902062/1‑94 (MQ=60)
                 CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             >  SRR4302535.3632149/1‑94 (MQ=60)
                 CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             <  SRR4302535.2011474/94‑1 (MQ=60)
                 CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             >  SRR4302535.3646854/1‑94 (MQ=60)
                 CTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATG                                                                             >  SRR4302535.4036528/1‑94 (MQ=60)
                  TGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGT                                                                            >  SRR4302535.2597477/1‑94 (MQ=60)
                   GGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTG                                                                           >  SRR4302535.120160/1‑94 (MQ=60)
                      GAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAAT                                                                        >  SRR4302535.2029612/1‑94 (MQ=60)
                      GAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAAT                                                                        <  SRR4302535.361726/94‑1 (MQ=60)
                        ATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCA                                                                      <  SRR4302535.12256/94‑1 (MQ=60)
                          GCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACT                                                                    <  SRR4302535.4655427/94‑1 (MQ=60)
                           CAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTT                                                                   <  SRR4302535.2155769/94‑1 (MQ=60)
                           CAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTT                                                                   >  SRR4302535.3293176/1‑94 (MQ=60)
                            AGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTC                                                                  >  SRR4302535.1068422/1‑94 (MQ=60)
                            AGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTC                                                                  >  SRR4302535.2025081/1‑94 (MQ=60)
                             GCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCA                                                                 <  SRR4302535.1933326/94‑1 (MQ=60)
                             ACAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCA                                                                 <  SRR4302535.3696240/94‑1 (MQ=60)
                              CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                <  SRR4302535.1124047/94‑1 (MQ=60)
                              CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                >  SRR4302535.4191710/1‑94 (MQ=60)
                              CAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCAC                                                                >  SRR4302535.2427139/1‑94 (MQ=60)
                               AGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATTTGAATCACTTCACA                                                               >  SRR4302535.1055895/1‑94 (MQ=60)
                                GTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACAC                                                              >  SRR4302535.2436522/1‑94 (MQ=60)
                                  CAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAA                                                            >  SRR4302535.711800/1‑94 (MQ=60)
                                  CAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAA                                                            >  SRR4302535.4701733/1‑94 (MQ=60)
                                    GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACCAAC                                                          >  SRR4302535.3987227/1‑94 (MQ=60)
                                    GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAC                                                          >  SRR4302535.866955/1‑94 (MQ=60)
                                    GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAC                                                          <  SRR4302535.685407/94‑1 (MQ=60)
                                    GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAC                                                          <  SRR4302535.548385/94‑1 (MQ=60)
                                    GTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAAC                                                          >  SRR4302535.1317119/1‑94 (MQ=60)
                                     TCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACA                                                         <  SRR4302535.3940003/94‑1 (MQ=60)
                                      CATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGTCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAG                                                        >  SRR4302535.4279919/1‑94 (MQ=60)
                                        TTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCC                                                      <  SRR4302535.165140/94‑1 (MQ=60)
                                        TTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCC                                                      <  SRR4302535.196096/94‑1 (MQ=60)
                                         TTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCG                                                     <  SRR4302535.4230463/94‑1 (MQ=60)
                                         TTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGGGAATCACTTCACACAAACAGCCG                                                     >  SRR4302535.3778746/1‑94 (MQ=60)
                                           TTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGT                                                   >  SRR4302535.2446943/1‑94 (MQ=60)
                                           TTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGT                                                   <  SRR4302535.3891657/94‑1 (MQ=60)
                                            TCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTT                                                  >  SRR4302535.737081/1‑94 (MQ=60)
                                             CTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTC                                                 >  SRR4302535.3188827/1‑94 (MQ=60)
                                              TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                <  SRR4302535.2396884/94‑1 (MQ=60)
                                              TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                <  SRR4302535.3799777/94‑1 (MQ=60)
                                              TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                >  SRR4302535.658301/1‑94 (MQ=60)
                                              TGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCG                                                >  SRR4302535.1761807/1‑94 (MQ=60)
                                               GCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGG                                               <  SRR4302535.2918161/94‑1 (MQ=60)
                                                CAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGT                                              >  SRR4302535.2775230/1‑94 (MQ=60)
                                                 AATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTT                                             >  SRR4302535.872679/1‑94 (MQ=60)
                                                 AATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATAACTTCACACAAACAGCCGGTTCGGTT                                             >  SRR4302535.3642850/1‑94 (MQ=60)
                                                  ATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTG                                            <  SRR4302535.2113681/94‑1 (MQ=60)
                                                   TTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGA                                           >  SRR4302535.3117264/1‑94 (MQ=60)
                                                   TTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGA                                           >  SRR4302535.1806416/1‑94 (MQ=60)
                                                    TTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAA                                          >  SRR4302535.4549391/1‑94 (MQ=58)
                                                     TTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAG                                         <  SRR4302535.2422294/94‑1 (MQ=55)
                                                     TTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAG                                         >  SRR4302535.934889/1‑94 (MQ=55)
                                                      TTCTATTGCGGCCTGCGGAGAGCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGA                                        <  SRR4302535.4259987/94‑1 (MQ=41)
                                                      TTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGA                                        <  SRR4302535.1251718/94‑1 (MQ=53)
                                                        CTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGA                                      >  SRR4302535.3657597/1‑94 (MQ=48)
                                                        CTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGA                                      >  SRR4302535.4660034/1‑94 (MQ=48)
                                                         TATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAA                                     >  SRR4302535.3675530/1‑94 (MQ=45)
                                                         TATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAA                                     >  SRR4302535.1280745/1‑94 (MQ=45)
                                                         TATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAA                                     >  SRR4302535.2360633/1‑94 (MQ=45)
                                                          ATTGCGGCCTGCGGAGAACTCGCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAA                                    >  SRR4302535.1328018/1‑94 (MQ=41)
                                                             GCGGCCTTCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAAT                                 <  SRR4302535.4623935/94‑1 (MQ=22)
                                                              CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                <  SRR4302535.532299/94‑1 (MQ=33)
                                                              CGGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATC                                <  SRR4302535.854476/94‑1 (MQ=33)
                                                               GGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCC                               <  SRR4302535.2517931/94‑1 (MQ=30)
                                                               GGCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCC                               >  SRR4302535.1348622/1‑94 (MQ=30)
                                                                GCCTGCGGAGATCTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAATCAGCCGGTTCGGTTGAAGAGAAAAATCCT                              <  SRR4302535.4638422/94‑1 (MQ=13)
                                                                GCCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCT                              >  SRR4302535.74740/1‑94 (MQ=28)
                                                                 CCTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTG                             <  SRR4302535.3611684/94‑1 (MQ=25)
                                                                  CTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGA                            <  SRR4302535.3441213/94‑1 (MQ=23)
                                                                  CTGCGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATATGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGA                            >  SRR4302535.327499/1‑94 (MQ=22)
                                                                     CGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAT                         >  SRR4302535.4166828/1‑94 (MQ=12)
                                                                     CGGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAAT                         <  SRR4302535.2058295/94‑1 (MQ=12)
                                                                      GGAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATT                        <  SRR4302535.3948441/94‑1 (MQ=10)
                                                                       GAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTC                       <  SRR4302535.4018266/94‑1 (MQ=7)
                                                                       GAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTC                       >  SRR4302535.3522017/1‑94 (MQ=7)
                                                                       GAGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTC                       <  SRR4302535.1177900/94‑1 (MQ=7)
                                                                        AGAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCA                      >  SRR4302535.216529/1‑94 (MQ=5)
                                                                         GAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG                     >  SRR4302535.773256/1‑94 (MQ=2)
                                                                         GAACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAG                     >  SRR4302535.2521316/1‑94 (MQ=2)
                                                                          AACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGG                    <  SRR4302535.1721597/94‑1 (MQ=0)
                                                                           ACTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGG                   <  SRR4302535.3942162/94‑1 (MQ=0)
                                                                            CTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGT                  <  SRR4302535.2506889/94‑1 (MQ=0)
                                                                            CTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGT                  >  SRR4302535.2899984/1‑94 (MQ=0)
                                                                            CTCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGT                  <  SRR4302535.3280600/94‑1 (MQ=0)
                                                                             TCCCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTT                 <  SRR4302535.4565735/94‑1 (MQ=0)
                                                                              CCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTG                <  SRR4302535.2759265/94‑1 (MQ=0)
                                                                               CCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGATAAATCCTGAAATTCAGGGTTGA               >  SRR4302535.4207378/1‑94 (MQ=0)
                                                                               CCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGA               >  SRR4302535.4588486/1‑94 (MQ=0)
                                                                               CCTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGA               >  SRR4302535.1971713/1‑94 (MQ=0)
                                                                                CTATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAC              >  SRR4302535.3571215/1‑94 (MQ=0)
                                                                                 TATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACT             >  SRR4302535.3771567/1‑94 (MQ=0)
                                                                                  ATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTC            >  SRR4302535.4649100/1‑94 (MQ=0)
                                                                                  ATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTC            >  SRR4302535.1619148/1‑94 (MQ=0)
                                                                                  ATAATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTC            >  SRR4302535.3543598/1‑94 (MQ=0)
                                                                                    AATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTG          >  SRR4302535.3839034/1‑94 (MQ=0)
                                                                                    AATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTG          >  SRR4302535.40517/1‑94 (MQ=0)
                                                                                     ATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGA         >  SRR4302535.3704474/1‑94 (MQ=0)
                                                                                     ATGCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGA         >  SRR4302535.4185255/1‑94 (MQ=0)
                                                                                      TGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAA        <  SRR4302535.423447/94‑1 (MQ=0)
                                                                                       GCGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAA       <  SRR4302535.381884/94‑1 (MQ=0)
                                                                                        CGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAG      <  SRR4302535.2694397/94‑1 (MQ=0)
                                                                                        CGCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAG      >  SRR4302535.3294675/1‑94 (MQ=0)
                                                                                         GCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGA     <  SRR4302535.4297359/94‑1 (MQ=0)
                                                                                         GCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGA     <  SRR4302535.2014139/94‑1 (MQ=0)
                                                                                         GCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGA     >  SRR4302535.2658435/1‑94 (MQ=0)
                                                                                         GCCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGA     >  SRR4302535.3264822/1‑94 (MQ=0)
                                                                                          CCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302535.739031/1‑94 (MQ=0)
                                                                                          CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302535.2007361/1‑94 (MQ=0)
                                                                                          CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302535.1782909/1‑94 (MQ=0)
                                                                                          CCCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAG    >  SRR4302535.3115519/1‑94 (MQ=0)
                                                                                           CTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   >  SRR4302535.821770/1‑94 (MQ=0)
                                                                                           CCCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGG   <  SRR4302535.26245/94‑1 (MQ=0)
                                                                                            TCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  SRR4302535.2010503/1‑94 (MQ=0)
                                                                                            |                                                                                             
TATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGA  >  NC_000913/4205346‑4205531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑