Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,494	A→G	Y107C (TAC→TGC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,494	0	A	G	99.0%	642.1 / NA	206	Y107C (TAC→TGC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (104/100); minor base T (1/1); total (105/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216404‑4216583

 aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTg                                                                                        >  1:4280404/1‑93 (MQ=255)

 aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTg                                                                                        >  1:1393879/1‑93 (MQ=255)

 aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTg                                                                                        >  1:2570977/1‑93 (MQ=255)

 aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTg                                                                                        >  2:2724878/1‑93 (MQ=255)

    tttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCg                                                                                     >  1:792113/1‑93 (MQ=255)

    tttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCg                                                                                     <  1:2385579/93‑1 (MQ=255)

        ctacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTa                                                                                 <  2:2474938/93‑1 (MQ=255)

        ctacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTa                                                                                 <  2:1719921/93‑1 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                               >  2:3909470/1‑93 (MQ=255)

           cGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAttt                                                                              <  2:2000037/93‑1 (MQ=255)

           cGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAttt                                                                              <  2:1010254/93‑1 (MQ=255)

            gtgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTg                                                                             >  1:4118273/1‑93 (MQ=255)

             tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGa                                                                            <  1:3076282/93‑1 (MQ=255)

             tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGa                                                                            <  1:2145540/93‑1 (MQ=255)

             tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGa                                                                            >  1:1494148/1‑93 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                           >  1:989158/1‑93 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                           >  1:955089/1‑93 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                           <  2:3144823/93‑1 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                           >  2:841272/1‑93 (MQ=255)

                tAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGacca                                                                         <  1:885952/93‑1 (MQ=255)

                  aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGaccacc                                                                       >  2:3811361/1‑93 (MQ=255)

                   agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcg                                                                      >  1:4627744/1‑93 (MQ=255)

                   agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcg                                                                      >  1:1229111/1‑93 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                     >  2:2380963/1‑93 (MQ=255)

                      gCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCTAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctc                                                                   <  2:4634407/93‑1 (MQ=255)

                      gCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctc                                                                   <  2:1445990/93‑1 (MQ=255)

                        aTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgc                                                                 <  2:1870533/93‑1 (MQ=255)

                        aTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgc                                                                 <  1:3445131/93‑1 (MQ=255)

                        aTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAAATCCGTGTGCTGTCGGTTATTTGACCACcgctcgc                                                                 >  2:399427/1‑93 (MQ=255)

                         ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgct                                                                <  1:632283/93‑1 (MQ=255)

                         ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgct                                                                >  1:363943/1‑93 (MQ=255)

                         ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgct                                                                <  2:3636301/93‑1 (MQ=255)

                          tACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCtt                                                               <  2:36575/93‑1 (MQ=255)

                           acacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTa                                                              <  1:3788988/93‑1 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                             >  2:1902834/1‑93 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                             <  1:2827106/93‑1 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                             >  1:373546/1‑93 (MQ=255)

                             acCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACt                                                            <  2:2753679/93‑1 (MQ=255)

                              cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                           <  2:283795/93‑1 (MQ=255)

                               ccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTcc                                                          <  1:3863363/93‑1 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                         >  1:3340124/1‑93 (MQ=255)

                                 ggCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCg                                                        <  2:2941964/93‑1 (MQ=255)

                                  gCTGTTGCCGGATTTCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       <  2:3679508/93‑1 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     >  2:1228896/1‑93 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     >  2:1701148/1‑93 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     >  1:2656753/1‑93 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     >  1:2953500/1‑93 (MQ=255)

                                    cgTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     <  1:1380827/92‑1 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTTACCACCGCTCGCTTACTCCCGAGCg                                                    >  1:3976773/1‑93 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                    <  2:665899/93‑1 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                    <  1:1810158/93‑1 (MQ=255)

                                         ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                                <  2:4166324/93‑1 (MQ=255)

                                         ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                                >  1:2038602/1‑93 (MQ=255)

                                         ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                                <  2:78633/93‑1 (MQ=255)

                                          cGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCttt                                               <  2:1520793/93‑1 (MQ=255)

                                           ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtttt                                              <  2:1142511/93‑1 (MQ=255)

                                           ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtttt                                              <  2:468360/93‑1 (MQ=255)

                                             aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTGTTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                            <  1:3115771/93‑1 (MQ=255)

                                              ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                           <  2:1937426/93‑1 (MQ=255)

                                              ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                           <  1:3536872/93‑1 (MQ=255)

                                                aCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCt                                         <  1:730818/93‑1 (MQ=255)

                                                 cccGCGCTTCGAGATTGCGGAGATCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                        <  2:3351330/93‑1 (MQ=255)

                                                 cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                        <  2:875867/93‑1 (MQ=255)

                                                  ccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCttt                                       >  1:4211152/1‑93 (MQ=255)

                                                    gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                     <  2:1365717/93‑1 (MQ=255)

                                                     cgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGGTTACTCCCGAGCGGCTTTTTATCTTTAGc                                    <  1:4303135/93‑1 (MQ=255)

                                                      gcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGct                                   >  1:3281705/1‑93 (MQ=255)

                                                      gcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGct                                   >  1:4539725/1‑93 (MQ=255)

                                                       cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                                  <  2:2593367/93‑1 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                 >  1:2114333/1‑93 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                 >  1:2507495/1‑93 (MQ=255)

                                                         tCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCtctc                                <  2:3518622/93‑1 (MQ=255)

                                                           gagaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAg                              >  2:1178712/1‑93 (MQ=255)

                                                             gaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                            >  2:4089721/1‑93 (MQ=255)

                                                             gaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                            >  1:593519/1‑93 (MQ=255)

                                                              aTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                           <  1:2348227/93‑1 (MQ=255)

                                                                 gCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCagag                        >  2:402791/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTTCTGTCGGTTATTTGCCCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAACCCGAtcg                      >  2:370257/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCTCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  1:4686599/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      <  2:924192/93‑1 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  1:2256829/1‑93 (MQ=255)

                                                                     agagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgcc                    >  1:3382647/1‑93 (MQ=255)

                                                                      gagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                   <  1:449567/93‑1 (MQ=255)

                                                                       ggCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGGCCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccgc                  <  1:1308640/92‑1 (MQ=255)

                                                                        gCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCt                 >  1:2189276/1‑93 (MQ=255)

                                                                          ttttttAGCTCCGTGTGCTGTCTGTTATTTGACCACCGGTCGCTTACTCCCGAGCGGGTTTTTATCTTTAGCTCTCAGCCAAAGCGCCGCttt               <  2:3438009/93‑1 (MQ=255)

                                                                          ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt               >  1:2246104/1‑93 (MQ=255)

                                                                          ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt               >  2:1647507/1‑93 (MQ=255)

                                                                           tttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTc              >  2:3341457/1‑93 (MQ=255)

                                                                            ttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCg             >  2:4446180/1‑93 (MQ=255)

                                                                                aaCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc         >  2:2293057/1‑93 (MQ=255)

                                                                                  cTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAt       >  1:455507/1‑93 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt      <  2:1079628/93‑1 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt      >  1:2860623/1‑93 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt      >  1:3404034/1‑93 (MQ=255)

                                                                                    ccGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc     <  1:4598028/93‑1 (MQ=255)

                                                                                    ccGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc     <  2:571161/93‑1 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc   >  1:135213/1‑93 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc   <  2:1021793/93‑1 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc   >  1:106065/1‑93 (MQ=255)

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216404‑4216583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  >  NC_000913/4216401‑4216587

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTG                                                                                               <  SRR4302535.2477150/94‑1 (MQ=60)

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTG                                                                                               <  SRR4302535.3452364/94‑1 (MQ=60)

 GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGC                                                                                              >  SRR4302535.940393/1‑94 (MQ=60)

 GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGC                                                                                              <  SRR4302535.2610224/94‑1 (MQ=60)

  GCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCT                                                                                             <  SRR4302535.2597871/94‑1 (MQ=60)

  GCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCT                                                                                             <  SRR4302535.3151702/94‑1 (MQ=60)

  GCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCT                                                                                             <  SRR4302535.3652515/94‑1 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            <  SRR4302535.2210336/94‑1 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            >  SRR4302535.2589460/1‑94 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            >  SRR4302535.2599185/1‑94 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            >  SRR4302535.1403834/1‑94 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            >  SRR4302535.4309692/1‑94 (MQ=60)

    ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGT                                                                                           <  SRR4302535.98413/94‑1 (MQ=60)

    ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGT                                                                                           <  SRR4302535.2389485/94‑1 (MQ=60)

      TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTTACTCCGTGTGCTGTCG                                                                                         >  SRR4302535.1091377/1‑94 (MQ=60)

      TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCG                                                                                         >  SRR4302535.797676/1‑94 (MQ=60)

       TTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGG                                                                                        <  SRR4302535.2402721/94‑1 (MQ=60)

        TTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGT                                                                                       >  SRR4302535.1268389/1‑94 (MQ=60)

         TACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTT                                                                                      <  SRR4302535.3413274/94‑1 (MQ=60)

           CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAT                                                                                    >  SRR4302535.4175914/1‑94 (MQ=60)

             ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTT                                                                                  <  SRR4302535.4298739/94‑1 (MQ=60)

             ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTT                                                                                  >  SRR4302535.66615/1‑94 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 >  SRR4302535.4146459/1‑94 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 <  SRR4302535.3242651/94‑1 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 >  SRR4302535.997556/1‑94 (MQ=60)

               GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGA                                                                                >  SRR4302535.1504889/1‑94 (MQ=60)

                TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGAC                                                                               <  SRR4302535.3097743/94‑1 (MQ=60)

                TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGAC                                                                               >  SRR4302535.996182/1‑94 (MQ=60)

                TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGAC                                                                               <  SRR4302535.2160976/94‑1 (MQ=60)

                TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGAC                                                                               >  SRR4302535.961857/1‑94 (MQ=60)

                   TAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCAC                                                                            <  SRR4302535.892221/94‑1 (MQ=60)

                    AAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACC                                                                           >  SRR4302535.3149148/1‑94 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          >  SRR4302535.4659157/1‑94 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          >  SRR4302535.1237893/1‑94 (MQ=60)

                       GAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCT                                                                        <  SRR4302535.2436693/94‑1 (MQ=60)

                       GAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCT                                                                        <  SRR4302535.2580004/94‑1 (MQ=60)

                        AGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTC                                                                       >  SRR4302535.3827308/1‑94 (MQ=60)

                           ATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCT                                                                    >  SRR4302535.366451/1‑94 (MQ=60)

                           ATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCT                                                                    <  SRR4302535.3469087/94‑1 (MQ=60)

                            TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTT                                                                   <  SRR4302535.636689/94‑1 (MQ=60)

                              ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAC                                                                 >  SRR4302535.376132/1‑94 (MQ=60)

                              ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAC                                                                 <  SRR4302535.3815121/94‑1 (MQ=60)

                              ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAC                                                                 <  SRR4302535.4589421/94‑1 (MQ=60)

                               CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACT                                                                >  SRR4302535.2271654/1‑94 (MQ=60)

                               CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACT                                                                <  SRR4302535.2847171/94‑1 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             >  SRR4302535.3436567/1‑94 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             >  SRR4302535.1618608/1‑94 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             <  SRR4302535.3889942/94‑1 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             >  SRR4302535.3363399/1‑94 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             >  SRR4302535.3750868/1‑94 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             <  SRR4302535.921834/94‑1 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                         >  SRR4302535.2974249/1‑94 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                         <  SRR4302535.404909/94‑1 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                         >  SRR4302535.2675788/1‑94 (MQ=60)

                                       TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTTACCACCGCTCGCTTACTCCCGAGCG                                                        >  SRR4302535.4003942/1‑94 (MQ=60)

                                       TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                        <  SRR4302535.4521119/94‑1 (MQ=60)

                                       TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                        >  SRR4302535.3543406/1‑94 (MQ=60)

                                       CGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                        <  SRR4302535.1390673/94‑1 (MQ=60)

                                        GTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGG                                                       <  SRR4302535.1823251/94‑1 (MQ=60)

                                          TGCCCGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTGTAACTCCGTGTTCTGGCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCT                                                     <  SRR4302535.397787/94‑1 (MQ=60)

                                           GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTT                                                    >  SRR4302535.2053281/1‑94 (MQ=60)

                                            CCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTT                                                   <  SRR4302535.3069235/94‑1 (MQ=60)

                                              GGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTT                                                 >  SRR4302535.2721239/1‑94 (MQ=60)

                                                ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTGTTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAT                                               <  SRR4302535.3137463/94‑1 (MQ=60)

                                                 TTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATC                                              <  SRR4302535.3561425/94‑1 (MQ=60)

                                                  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                             <  SRR4302535.614787/94‑1 (MQ=60)

                                                  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                             <  SRR4302535.849873/94‑1 (MQ=60)

                                                   ACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                            <  SRR4302535.4404309/94‑1 (MQ=60)

                                                   ACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                            <  SRR4302535.735951/94‑1 (MQ=60)

                                                   ACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                            >  SRR4302535.2926341/1‑94 (MQ=60)

                                                    CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                           >  SRR4302535.4559582/1‑94 (MQ=60)

                                                    CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                           >  SRR4302535.4239972/1‑94 (MQ=60)

                                                      CGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAG                                         <  SRR4302535.3333487/94‑1 (MQ=60)

                                                      CGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAG                                         <  SRR4302535.1999914/94‑1 (MQ=60)

                                                       GCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGC                                        <  SRR4302535.3632706/94‑1 (MQ=60)

                                                       GCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGC                                        <  SRR4302535.2146164/94‑1 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGGTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       <  SRR4302535.4332572/94‑1 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       >  SRR4302535.1401136/1‑94 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       >  SRR4302535.4570777/1‑94 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       >  SRR4302535.3773790/1‑94 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       >  SRR4302535.3304496/1‑94 (MQ=60)

                                                          CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                     >  SRR4302535.2129592/1‑94 (MQ=60)

                                                          CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                     >  SRR4302535.2525572/1‑94 (MQ=60)

                                                             CGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                                  <  SRR4302535.2404420/94‑1 (MQ=60)

                                                               AGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                                >  SRR4302535.597681/1‑94 (MQ=60)

                                                               AGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                                >  SRR4302535.4318251/1‑94 (MQ=60)

                                                                GATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                               <  SRR4302535.1372129/94‑1 (MQ=60)

                                                                 ATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAG                              <  SRR4302535.2365062/94‑1 (MQ=60)

                                                                     CGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCTCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                          >  SRR4302535.4718379/1‑94 (MQ=60)

                                                                     CGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                          >  SRR4302535.2272895/1‑94 (MQ=60)

                                                                      GGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGC                         >  SRR4302535.1462125/1‑94 (MQ=60)

                                                                       GAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCC                        >  SRR4302535.3406163/1‑94 (MQ=60)

                                                                        AGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                       >  SRR4302535.371849/1‑94 (MQ=60)

                                                                         GAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                      >  SRR4302535.4639936/1‑94 (MQ=60)

                                                                         GAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                      <  SRR4302535.452715/94‑1 (MQ=60)

                                                                          GGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGGCCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     <  SRR4302535.1317991/94‑1 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     >  SRR4302535.588796/1‑94 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     >  SRR4302535.2204866/1‑94 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     <  SRR4302535.1924254/94‑1 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     >  SRR4302535.233172/1‑94 (MQ=60)

                                                                           GCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTT                    <  SRR4302535.2053542/94‑1 (MQ=60)

                                                                            CTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTT                   >  SRR4302535.2262075/1‑94 (MQ=60)

                                                                                TTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTA               <  SRR4302535.3118896/94‑1 (MQ=60)

                                                                                TTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTA               >  SRR4302535.2853152/1‑94 (MQ=60)

                                                                                 TTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAC              <  SRR4302535.4679188/94‑1 (MQ=60)

                                                                                   AACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA            <  SRR4302535.1351915/94‑1 (MQ=60)

                                                                                    ACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAT           >  SRR4302535.458694/1‑94 (MQ=60)

                                                                                     CTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT          >  SRR4302535.3427725/1‑94 (MQ=60)

                                                                                     CTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT          <  SRR4302535.3377551/94‑1 (MQ=60)

                                                                                     CTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT          >  SRR4302535.2880887/1‑94 (MQ=60)

                                                                                      TCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC         <  SRR4302535.2201299/94‑1 (MQ=60)

                                                                                       CCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCC        <  SRR4302535.4629308/94‑1 (MQ=60)

                                                                                        CGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC       >  SRR4302535.136158/1‑94 (MQ=60)

                                                                                        CGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC       >  SRR4302535.4582541/1‑94 (MQ=60)

                                                                                        CGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC       >  SRR4302535.106819/1‑94 (MQ=60)

                                                                                         GTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC      >  SRR4302535.774130/1‑94 (MQ=60)

                                                                                          TGTTCTGTCGGTTATTTGACCCCCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCG     <  SRR4302535.722659/94‑1 (MQ=60)

                                                                                           GTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGC    <  SRR4302535.3649927/94‑1 (MQ=60)

                                                                                           GTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGC    >  SRR4302535.184221/1‑94 (MQ=60)

                                                                                             GCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  >  SRR4302535.2145114/1‑94 (MQ=60)

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  >  NC_000913/4216401‑4216587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`