Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,239,076 A→G V253A (GTA→GCA)  xylE ← xylose‑proton symport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,239,0760AG100.0% 776.0 / NA 242V253A (GTA→GCA) xylExylose‑proton symport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (122/120);  total (122/120)

Only 100 of 256 total aligned reads displayed.

ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA  >  NC_000913/4238986‑4239165
                                                                                          |                                                                                         
 ttACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTg                                                                                        >  1:3986588/1‑93 (MQ=255)
   aCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGcc                                                                                      <  2:347560/93‑1 (MQ=255)
   aCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGcc                                                                                      <  2:4687365/93‑1 (MQ=255)
    cGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCt                                                                                     <  2:619243/93‑1 (MQ=255)
     gCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTg                                                                                    <  2:378095/93‑1 (MQ=255)
       cGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAg                                                                                  >  2:1748304/1‑93 (MQ=255)
       cGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAg                                                                                  >  2:2385077/1‑93 (MQ=255)
       cGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAg                                                                                  <  1:4547604/93‑1 (MQ=255)
         aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGtt                                                                                <  1:3154990/93‑1 (MQ=255)
         aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCGGTGCTGCCTGAgcg                                                                                >  2:2188648/1‑91 (MQ=255)
           tACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGc                                                                              >  1:4137871/1‑93 (MQ=255)
           tACAACCACGCCCACGCCCAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTTCCTGAGTTGc                                                                              <  2:2617606/93‑1 (MQ=255)
            aCAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCa                                                                             >  2:3261622/1‑93 (MQ=255)
             cAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCaa                                                                            <  2:1865532/93‑1 (MQ=255)
              aaTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAg                                                                           >  2:3680044/1‑93 (MQ=255)
               aTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGc                                                                          >  2:4499334/1‑93 (MQ=255)
               aTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGc                                                                          <  1:3831344/93‑1 (MQ=255)
                  acgcccacgccAAACATCAGCAGACGACCACCGGGTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgtg                                                                       >  1:2127695/1‑93 (MQ=255)
                   cgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgtgt                                                                      <  1:4346310/93‑1 (MQ=255)
                   cgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgtgt                                                                      <  2:2693070/93‑1 (MQ=255)
                    gcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGtt                                                                     <  1:2645432/93‑1 (MQ=255)
                    gcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGtt                                                                     <  2:3430214/93‑1 (MQ=255)
                    gcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGtt                                                                     >  2:2205330/1‑93 (MQ=255)
                     cccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTg                                                                    <  2:2524411/93‑1 (MQ=255)
                     cccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTg                                                                    >  1:3738316/1‑93 (MQ=255)
                      ccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGc                                                                   >  1:2282341/1‑93 (MQ=255)
                      ccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGc                                                                   <  2:4193157/93‑1 (MQ=255)
                      ccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGc                                                                   >  1:4227681/1‑93 (MQ=255)
                       cacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGcc                                                                  >  2:2299896/1‑93 (MQ=255)
                       cacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGGTTAATTTACTGTGCTGCCTGAGTTACAAGCGTCTTGcc                                                                  >  2:3179865/1‑93 (MQ=255)
                        acgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGccc                                                                 <  1:803725/93‑1 (MQ=255)
                            cAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATaa                                                             <  1:763142/93‑1 (MQ=255)
                            cAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATaa                                                             >  2:1802958/1‑93 (MQ=255)
                             aaaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAt                                                            >  1:3962941/1‑93 (MQ=255)
                             aaaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAt                                                            <  2:2266338/93‑1 (MQ=255)
                              aaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAtt                                                           <  2:4639640/93‑1 (MQ=255)
                               aCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAttt                                                          <  1:2842155/93‑1 (MQ=255)
                                 aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                        <  2:1158089/93‑1 (MQ=255)
                                  tCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgc                                                       >  1:1021833/1‑93 (MQ=255)
                                     gcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCa                                                    <  1:4306686/93‑1 (MQ=255)
                                     gcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCa                                                    <  1:3528201/93‑1 (MQ=255)
                                      cagACGCCCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  2:572233/93‑1 (MQ=255)
                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   >  1:699260/1‑93 (MQ=255)
                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  2:8177/93‑1 (MQ=255)
                                        gacgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGa                                                 >  2:3924665/1‑93 (MQ=255)
                                        gacgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTGGCCCATAAGTTGGCGCAGGa                                                 <  2:3612968/93‑1 (MQ=255)
                                         acgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGAt                                                <  2:4434945/93‑1 (MQ=255)
                                          cgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATa                                               <  2:4072387/93‑1 (MQ=255)
                                           gacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAc                                              >  1:4659286/1‑93 (MQ=255)
                                           gacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAc                                              <  2:2824448/93‑1 (MQ=255)
                                             ccaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCt                                            <  1:907775/93‑1 (MQ=255)
                                             ccaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCt                                            <  1:3881632/93‑1 (MQ=255)
                                              caccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCtt                                           >  2:2931019/1‑93 (MQ=255)
                                               accGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTc                                          <  1:621932/93‑1 (MQ=255)
                                                ccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                         >  1:1319934/1‑93 (MQ=255)
                                                ccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                         <  2:1702559/93‑1 (MQ=255)
                                                ccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                         <  2:1337195/93‑1 (MQ=255)
                                                  ggTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGc                                       >  2:3848387/1‑93 (MQ=255)
                                                   gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                      >  2:3074225/1‑93 (MQ=255)
                                                   gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                      <  2:1484001/93‑1 (MQ=255)
                                                   gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                      <  1:2229051/93‑1 (MQ=255)
                                                     tttGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTg                                    <  1:2797637/93‑1 (MQ=255)
                                                         cGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTct                                >  2:663426/1‑93 (MQ=255)
                                                          ggCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTctt                               <  2:2777906/93‑1 (MQ=255)
                                                          ggCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTctt                               <  1:4484368/93‑1 (MQ=255)
                                                           gCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttg                              <  1:1553687/93‑1 (MQ=255)
                                                            ccATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgc                             <  1:1747378/93‑1 (MQ=255)
                                                              aTGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATGCCTTCCGCCTGTTcttgctt                           >  2:3051542/1‑93 (MQ=255)
                                                              aTGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgctt                           >  2:243849/1‑93 (MQ=255)
                                                              aTGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgctt                           <  2:3542186/93‑1 (MQ=255)
                                                                 aTCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGcc                        >  2:2753267/1‑93 (MQ=255)
                                                                 aTCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGcc                        <  1:3193928/93‑1 (MQ=255)
                                                                  tCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                       >  1:3648436/1‑93 (MQ=255)
                                                                    cAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTct                                >  1:2164873/1‑82 (MQ=255)
                                                                    cAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                     >  1:3557821/1‑93 (MQ=255)
                                                                    cAGGGAGGGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCctcg                     >  2:3561512/1‑93 (MQ=255)
                                                                    cAGGGAGGGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                     >  1:3265926/1‑93 (MQ=255)
                                                                     aGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcgg                    >  1:537895/1‑92 (MQ=255)
                                                                     aGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcgc                    >  2:909988/1‑93 (MQ=255)
                                                                        gAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGAc                 >  2:3341431/1‑93 (MQ=255)
                                                                        gAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGAc                 <  1:1881024/93‑1 (MQ=255)
                                                                         aGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACa                <  1:3095711/93‑1 (MQ=255)
                                                                           tgtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATc              >  1:1195888/1‑93 (MQ=255)
                                                                           tgtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATc              >  1:3395785/1‑93 (MQ=255)
                                                                           tggttAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATc              <  2:1454944/90‑1 (MQ=255)
                                                                            gtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATca             <  2:2888033/93‑1 (MQ=255)
                                                                            gtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATca             <  2:1954702/93‑1 (MQ=255)
                                                                            gtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATca             >  1:4058431/1‑93 (MQ=255)
                                                                             tttAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcag            >  2:2086646/1‑93 (MQ=255)
                                                                             tttAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcag            <  1:130708/93‑1 (MQ=255)
                                                                                 aTTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGGCGCGCGACATcagcaag        >  2:1251401/1‑93 (MQ=255)
                                                                                  tttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATCCCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccagc       <  1:1659126/93‑1 (MQ=255)
                                                                                  tttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccagc       >  2:4506906/1‑93 (MQ=255)
                                                                                  tttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccagc       >  1:1736704/1‑93 (MQ=255)
                                                                                   ttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCg      >  2:2981290/1‑93 (MQ=255)
                                                                                   ttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCg      >  1:3001899/1‑93 (MQ=255)
                                                                                    tCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGa     <  2:734252/93‑1 (MQ=255)
                                                                                     ccTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAg    >  2:4285255/1‑93 (MQ=255)
                                                                                      cTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAgg   >  1:3463099/1‑93 (MQ=255)
                                                                                       tgtgCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGa  <  2:245546/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA  >  NC_000913/4238986‑4239165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTT  >  NC_000913/4238983‑4239168
                                                                                             |                                                                                            
AGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTG                                                                                              >  SRR4302535.2959392/1‑94 (MQ=60)
AGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTG                                                                                              >  SRR4302535.296064/1‑94 (MQ=60)
AGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTG                                                                                              >  SRR4302535.1461474/1‑94 (MQ=60)
 GCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGC                                                                                             >  SRR4302535.1560726/1‑94 (MQ=60)
  CATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCT                                                                                            >  SRR4302535.2203228/1‑94 (MQ=60)
  CATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCT                                                                                            <  SRR4302535.917890/94‑1 (MQ=60)
  CATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCT                                                                                            <  SRR4302535.805677/94‑1 (MQ=60)
   ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTG                                                                                           <  SRR4302535.2919861/94‑1 (MQ=60)
   ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTG                                                                                           >  SRR4302535.4013848/1‑94 (MQ=60)
   ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTG                                                                                           >  SRR4302535.481036/1‑94 (MQ=60)
    TTACGCC‑AATACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCC                                                                                         <  SRR4302535.181575/94‑1 (MQ=60)
     TACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCC                                                                                         <  SRR4302535.4060693/94‑1 (MQ=60)
          CGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGT                                                                                    <  SRR4302535.4578697/94‑1 (MQ=60)
          CGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGT                                                                                    >  SRR4302535.3488781/1‑94 (MQ=60)
          CGAATACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGT                                                                                    <  SRR4302535.3558583/94‑1 (MQ=60)
           GATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTT                                                                                   <  SRR4302535.1230285/94‑1 (MQ=60)
           GATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTT                                                                                   >  SRR4302535.649324/1‑94 (MQ=60)
           GATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTT                                                                                   <  SRR4302535.4555234/94‑1 (MQ=60)
           GATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTT                                                                                   <  SRR4302535.3057094/94‑1 (MQ=60)
            ATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTG                                                                                  <  SRR4302535.4367615/94‑1 (MQ=60)
            ATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTG                                                                                  <  SRR4302535.1631444/94‑1 (MQ=60)
            ATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTG                                                                                  <  SRR4302535.4686491/94‑1 (MQ=60)
            ATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTG                                                                                  <  SRR4302535.3176905/94‑1 (MQ=60)
             TTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGC                                                                                 >  SRR4302535.4166208/1‑94 (MQ=60)
              TACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCA                                                                                >  SRR4302535.1505503/1‑94 (MQ=60)
               ACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAA                                                                               <  SRR4302535.2799822/94‑1 (MQ=60)
                CAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAG                                                                              <  SRR4302535.3109916/94‑1 (MQ=60)
                 AATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGC                                                                             >  SRR4302535.3447169/1‑94 (MQ=60)
                 AATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGGGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGC                                                                             >  SRR4302535.4011340/1‑94 (MQ=60)
                  ATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCG                                                                            <  SRR4302535.3857766/94‑1 (MQ=60)
                  ATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCG                                                                            <  SRR4302535.1708122/94‑1 (MQ=60)
                    CACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTG                                                                          <  SRR4302535.1718663/94‑1 (MQ=60)
                    CACGCCCACGCCAAACATCAGCAGACGACCACCGGGTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTG                                                                          >  SRR4302535.2143039/1‑94 (MQ=60)
                     ACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGT                                                                         <  SRR4302535.388967/94‑1 (MQ=60)
                      CGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTT                                                                        <  SRR4302535.4376048/94‑1 (MQ=60)
                       GCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCCGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTG                                                                       >  SRR4302535.52944/1‑94 (MQ=60)
                       GCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTG                                                                       >  SRR4302535.3764189/1‑94 (MQ=60)
                       GCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTG                                                                       <  SRR4302535.589905/94‑1 (MQ=60)
                       GCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTG                                                                       <  SRR4302535.2664379/94‑1 (MQ=60)
                       GCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTG                                                                       <  SRR4302535.2394016/94‑1 (MQ=60)
                        CCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGC                                                                      >  SRR4302535.4256612/1‑94 (MQ=60)
                        CCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGC                                                                      >  SRR4302535.2358428/1‑94 (MQ=60)
                        CCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGC                                                                      >  SRR4302535.2298645/1‑94 (MQ=60)
                         CCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCC                                                                     >  SRR4302535.1413718/1‑94 (MQ=60)
                           ACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCA                                                                   <  SRR4302535.809369/94‑1 (MQ=60)
                              CCAAACATCGGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAA                                                                >  SRR4302535.2850906/1‑94 (MQ=60)
                              CCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAA                                                                >  SRR4302535.2187699/1‑94 (MQ=60)
                               CAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAT                                                               <  SRR4302535.768518/94‑1 (MQ=60)
                               CAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAT                                                               >  SRR4302535.3990009/1‑94 (MQ=60)
                                  ACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT                                                            <  SRR4302535.2862312/94‑1 (MQ=60)
                                   CATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAAATTACTGTGCTGCCTGAGATGCAAGCGTGTTGCCCAGAATTTTG                                                           >  SRR4302535.134193/1‑94 (MQ=60)
                                    ATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGC                                                          >  SRR4302535.1029092/1‑94 (MQ=60)
                                       AGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCA                                                       <  SRR4302535.3284867/94‑1 (MQ=60)
                                       AGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCA                                                       >  SRR4302535.2134323/1‑94 (MQ=60)
                                        GCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAG                                                      <  SRR4302535.3552702/94‑1 (MQ=60)
                                        GCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAG                                                      <  SRR4302535.4336150/94‑1 (MQ=60)
                                        GCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAG                                                      >  SRR4302535.704166/1‑94 (MQ=60)
                                         CAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGG                                                     >  SRR4302535.2075595/1‑94 (MQ=60)
                                          AGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGA                                                    <  SRR4302535.3614947/94‑1 (MQ=60)
                                           GACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGAT                                                   <  SRR4302535.1224268/94‑1 (MQ=60)
                                            ACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATA                                                  <  SRR4302535.3974975/94‑1 (MQ=60)
                                             CGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAC                                                 >  SRR4302535.4690802/1‑94 (MQ=60)
                                              GACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACC                                                <  SRR4302535.787950/94‑1 (MQ=60)
                                              GACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACC                                                >  SRR4302535.766280/1‑94 (MQ=60)
                                              GACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACC                                                >  SRR4302535.3971166/1‑94 (MQ=60)
                                                CCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTT                                              <  SRR4302535.3852128/94‑1 (MQ=60)
                                                CCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTT                                              <  SRR4302535.3908268/94‑1 (MQ=60)
                                                CCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTT                                              <  SRR4302535.914219/94‑1 (MQ=60)
                                                  CCCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCC                                            <  SRR4302535.312952/94‑1 (MQ=60)
                                                  ACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCC                                            >  SRR4302535.378471/1‑94 (MQ=60)
                                                  ACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCC                                            >  SRR4302535.1329365/1‑94 (MQ=60)
                                                  ACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCC                                            <  SRR4302535.626252/94‑1 (MQ=60)
                                                   CCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCG                                           <  SRR4302535.1317365/94‑1 (MQ=60)
                                                      GTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCT                                        >  SRR4302535.558802/1‑94 (MQ=60)
                                                      GTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCT                                        >  SRR4302535.3376142/1‑94 (MQ=60)
                                                      GTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCT                                        <  SRR4302535.2244903/94‑1 (MQ=60)
                                                        TTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGT                                      <  SRR4302535.2817546/94‑1 (MQ=60)
                                                         TTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTT                                     <  SRR4302535.3254115/94‑1 (MQ=60)
                                                           GCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCT                                   <  SRR4302535.3464522/94‑1 (MQ=60)
                                                            CGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTT                                  >  SRR4302535.2832540/1‑94 (MQ=60)
                                                             GGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTG                                 <  SRR4302535.4515153/94‑1 (MQ=60)
                                                              GCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGC                                <  SRR4302535.1564883/94‑1 (MQ=60)
                                                               CCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCT                               <  SRR4302535.1760016/94‑1 (MQ=60)
                                                               CCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCT                               >  SRR4302535.357744/1‑94 (MQ=60)
                                                               CCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCT                               >  SRR4302535.4212617/1‑94 (MQ=60)
                                                                CATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTT                              >  SRR4302535.4463834/1‑94 (MQ=60)
                                                                CATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTT                              >  SRR4302535.832789/1‑94 (MQ=60)
                                                                    ATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCG                          >  SRR4302535.1144212/1‑94 (MQ=60)
                                                                    ATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCG                          >  SRR4302535.2148505/1‑94 (MQ=60)
                                                                    ATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCG                          <  SRR4302535.3216026/94‑1 (MQ=60)
                                                                    ATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCG                          >  SRR4302535.3673696/1‑94 (MQ=60)
                                                                      CCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTaccacgcaga                        >  SRR4302535.2180397/1‑84 (MQ=60)
                                                                      CCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCG                        >  SRR4302535.3582496/1‑94 (MQ=60)
                                                                      CCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCG                        >  SRR4302535.3161145/1‑94 (MQ=60)
                                                                      CCAGGGAGGGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCG                        >  SRR4302535.3288616/1‑94 (MQ=60)
                                                                       CAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGG                       >  SRR4302535.541651/1‑94 (MQ=60)
                                                                        AGGGAGTGTTTAATTTCCTGTGCTGCCTGAATTGCAAGCGTTGTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCG                      <  SRR4302535.3539367/94‑1 (MQ=60)
                                                                         GGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGA                     >  SRR4302535.4605377/1‑94 (MQ=60)
                                                                           GAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACA                   >  SRR4302535.1772010/1‑94 (MQ=60)
                                                                           GAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACA                   <  SRR4302535.1894584/94‑1 (MQ=60)
                                                                            AGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATCCCTTCCGCCTGTTCTTGCTTGCCGCGCGACAT                  <  SRR4302535.2264983/94‑1 (MQ=60)
                                                                            AGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAT                  >  SRR4302535.4264647/1‑94 (MQ=60)
                                                                            AGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAT                  >  SRR4302535.4031972/1‑94 (MQ=60)
                                                                            AGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAT                  >  SRR4302535.3205001/1‑94 (MQ=60)
                                                                            AGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAT                  <  SRR4302535.3117271/94‑1 (MQ=60)
                                                                             GTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATC                 >  SRR4302535.3419409/1‑94 (MQ=60)
                                                                             GTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATC                 >  SRR4302535.1204455/1‑94 (MQ=60)
                                                                             GTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATC                 <  SRR4302535.41079/94‑1 (MQ=60)
                                                                              TGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCA                >  SRR4302535.4086210/1‑94 (MQ=60)
                                                                                TTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGC              <  SRR4302535.4258907/94‑1 (MQ=60)
                                                                                TTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGC              <  SRR4302535.131625/94‑1 (MQ=60)
                                                                                 TTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCC             <  SRR4302535.3601908/94‑1 (MQ=60)
                                                                                    ATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGC          >  SRR4302535.1749257/1‑94 (MQ=60)
                                                                                    ATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGC          <  SRR4302535.3705513/94‑1 (MQ=60)
                                                                                     TTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATCCCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCG         <  SRR4302535.1671120/94‑1 (MQ=60)
                                                                                     TTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCG         >  SRR4302535.3022942/1‑94 (MQ=60)
                                                                                       TCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAG       >  SRR4302535.780408/1‑94 (MQ=60)
                                                                                        CCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGG      >  SRR4302535.3487170/1‑94 (MQ=60)
                                                                                        CCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGG      <  SRR4302535.4177783/94‑1 (MQ=60)
                                                                                        CCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGG      <  SRR4302535.1750046/94‑1 (MQ=60)
                                                                                         CTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA     <  SRR4302535.760138/94‑1 (MQ=60)
                                                                                         CTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA     >  SRR4302535.937537/1‑94 (MQ=60)
                                                                                          TGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGAC    <  SRR4302535.4505984/94‑1 (MQ=60)
                                                                                            TGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTT  >  SRR4302535.2486096/1‑94 (MQ=60)
                                                                                             |                                                                                            
AGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTT  >  NC_000913/4238983‑4239168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑