Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,241,465 T→C D397G (GAC→GGC)  malF ← MalF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,241,4650TC99.6% 705.4 / ‑5.1 226D397G (GAC→GGC) malFMalF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (106/119);  total (107/119)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 237 total aligned reads displayed.

GTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGC  >  NC_000913/4241375‑4241553
                                                                                          |                                                                                        
gTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGccc                                                                                        >  1:2260534/1‑93 (MQ=255)
gTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGccc                                                                                        >  2:471204/1‑93 (MQ=255)
 tCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCg                                                                                       >  2:2157227/1‑93 (MQ=255)
   aGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGa                                                                                     >  1:1677475/1‑93 (MQ=255)
   aGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGa                                                                                     >  2:552822/1‑93 (MQ=255)
     cGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAAt                                                                                   <  1:765018/93‑1 (MQ=255)
     cGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAAt                                                                                   <  2:665994/93‑1 (MQ=255)
       gTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCg                                                                                 <  2:799777/93‑1 (MQ=255)
        tttAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGc                                                                                <  1:4192556/93‑1 (MQ=255)
         ttAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCt                                                                               <  1:4123059/93‑1 (MQ=255)
          tAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCtt                                                                              <  2:3200742/93‑1 (MQ=255)
          tAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCtt                                                                              >  1:3341565/1‑93 (MQ=255)
          tAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCtt                                                                              >  2:4098681/1‑93 (MQ=255)
           aaTCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCttt                                                                             >  2:3862175/1‑93 (MQ=255)
            aTCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTc                                                                            <  1:1746375/93‑1 (MQ=255)
             tCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCa                                                                           <  2:2652395/93‑1 (MQ=255)
              cagctgCGGCAGCGTAATCTTAAAGAAGTTCTGGACCGGCCCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAg                                                                          <  1:264341/93‑1 (MQ=255)
               agcagcGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGc                                                                         <  1:2902427/93‑1 (MQ=255)
                gcagcggcagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCa                                                                        >  2:348797/1‑93 (MQ=255)
                 cagcggcagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCaa                                                                       <  1:2890698/93‑1 (MQ=255)
                   gcggcagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGc                                                                     <  1:441657/93‑1 (MQ=255)
                    cggcagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAgtc                                                                    >  2:2886255/1‑91 (MQ=255)
                    cggcagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGcc                                                                    <  1:3696185/93‑1 (MQ=255)
                       cagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGCGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCAt                                                                 >  1:79066/1‑93 (MQ=255)
                       cagcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCAt                                                                 <  1:692365/93‑1 (MQ=255)
                        agcgTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATg                                                                >  2:1591871/1‑93 (MQ=255)
                           gTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGTCCATGCag                                                             >  2:1607653/1‑93 (MQ=255)
                            tAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCaga                                                            >  2:2974108/1‑93 (MQ=255)
                            tAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCaga                                                            <  1:1628196/93‑1 (MQ=255)
                            tAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCaga                                                            >  2:1245018/1‑93 (MQ=255)
                            tAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCaga                                                            >  1:4520660/1‑93 (MQ=255)
                              aTCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAgg                                                          >  2:3167662/1‑93 (MQ=255)
                               tCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGa                                                         <  1:1810165/93‑1 (MQ=255)
                               tCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGa                                                         >  1:1185761/1‑93 (MQ=255)
                                  tAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatca                                                      <  1:1013656/93‑1 (MQ=255)
                                    aagaagTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatcatc                                                    >  1:849683/1‑93 (MQ=255)
                                    aagaagTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatcatc                                                    <  1:1119732/93‑1 (MQ=255)
                                    aagaagTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGatcatc                                                    <  2:4422957/93‑1 (MQ=255)
                                     agaagTTCTGTAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGTAATCGCTTTCAGCAAGCCTATGCAGAGGAtcatca                                                   <  2:1299660/93‑1 (MQ=255)
                                         gTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTa                                               <  2:757484/93‑1 (MQ=255)
                                         gTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTa                                               <  2:635637/93‑1 (MQ=255)
                                          ttCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTAc                                              >  2:821635/1‑93 (MQ=255)
                                          ttCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTAc                                              <  2:71511/93‑1 (MQ=255)
                                             tGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGGTGATCATGTACGGa                                           >  2:91346/1‑93 (MQ=255)
                                             tGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGa                                           <  2:1098667/93‑1 (MQ=255)
                                             tGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGa                                           <  2:326795/93‑1 (MQ=255)
                                               gAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATa                                         >  2:2025354/1‑93 (MQ=255)
                                                aaCGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATaa                                        <  2:1481611/93‑1 (MQ=255)
                                                 aCGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAc                                       >  2:2468923/1‑93 (MQ=255)
                                                 aCGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAc                                       >  2:3613698/1‑93 (MQ=255)
                                                   ggACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAccc                                     >  1:194321/1‑93 (MQ=255)
                                                    gACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACcca                                    <  1:3236900/93‑1 (MQ=255)
                                                    gACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACcca                                    >  1:1865865/1‑93 (MQ=255)
                                                       cTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAATCCCATGCAGAGGATCATCATGTACGGATAACccagcc                                 <  1:2099224/93‑1 (MQ=255)
                                                       cTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACccagcc                                 >  1:1385806/1‑93 (MQ=255)
                                                        tGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACccagcca                                <  2:1716009/93‑1 (MQ=255)
                                                         gcgcCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACccagccag                               <  2:2803519/93‑1 (MQ=255)
                                                         gcgcCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACccagccag                               <  1:2321157/93‑1 (MQ=255)
                                                           gcCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGt                             <  2:1349084/93‑1 (MQ=255)
                                                           gcCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGt                             >  2:3993393/1‑93 (MQ=255)
                                                            ccatccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTa                            >  1:4634548/1‑93 (MQ=255)
                                                              atccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAtt                          <  2:1791303/93‑1 (MQ=255)
                                                              atccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAtt                          >  1:2924889/1‑93 (MQ=255)
                                                              atccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAtt                          >  2:4325299/1‑93 (MQ=255)
                                                               tccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTg                         >  1:2554540/1‑93 (MQ=255)
                                                               tccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTg                         >  2:2623456/1‑93 (MQ=255)
                                                                ccatTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGa                        >  2:4162463/1‑93 (MQ=255)
                                                                  atTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACg                      <  1:2485780/93‑1 (MQ=255)
                                                                  atTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACg                      <  2:3405179/93‑1 (MQ=255)
                                                                  atTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACg                      >  2:2842684/1‑93 (MQ=255)
                                                                   ttGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGAAGa                     >  1:3863069/1‑93 (MQ=255)
                                                                    tGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGAt                    <  1:4350577/93‑1 (MQ=255)
                                                                     gCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATa                   >  1:30907/1‑93 (MQ=255)
                                                                      cTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATaa                  <  1:1535442/93‑1 (MQ=255)
                                                                        gAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAAtt                <  1:3966387/93‑1 (MQ=255)
                                                                        gAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAAtt                >  2:3741758/1‑93 (MQ=255)
                                                                         aGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTa               <  1:283329/93‑1 (MQ=255)
                                                                         aGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTa               <  1:1353834/93‑1 (MQ=255)
                                                                          ggCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAg              >  2:3458741/1‑93 (MQ=255)
                                                                          ggCTTCATACAAATCGCCCGGAATCCCTTTCAGCAAGCCCATTCAGAGGATCATCATGTACCGTTAACCCATCCAGGGATTTACGATAATTAg              >  2:3710853/1‑93 (MQ=255)
                                                                           gCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGc             <  2:2740824/93‑1 (MQ=255)
                                                                           gCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGc             >  2:4602462/1‑93 (MQ=255)
                                                                            cTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCa            <  1:4542341/93‑1 (MQ=255)
                                                                            cTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCa            <  2:499683/93‑1 (MQ=255)
                                                                             ttCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCAt           >  2:1547090/1‑93 (MQ=255)
                                                                             ttCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCAt           <  2:2413822/93‑1 (MQ=255)
                                                                              tCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATc          <  2:1345754/93‑1 (MQ=255)
                                                                              tCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATc          <  2:1599853/93‑1 (MQ=255)
                                                                               cATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCg         >  2:2122099/1‑93 (MQ=255)
                                                                                aTACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGt        >  1:543335/1‑93 (MQ=255)
                                                                                aTACAAATCGCCCGGAATCGCTTTCAGCAAGCCCACGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGt        >  2:4084721/1‑93 (MQ=255)
                                                                                 tACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTg       >  1:1276682/1‑93 (MQ=255)
                                                                                  aCAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgc      >  1:2133818/1‑93 (MQ=255)
                                                                                  aCAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgc      <  1:2209588/93‑1 (MQ=255)
                                                                                  aCAAAGCGCCCGGAATCGCGTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCAGCGTgc      <  2:3039473/93‑1 (MQ=255)
                                                                                   cAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgcg     <  1:303589/93‑1 (MQ=255)
                                                                                    aaaTCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgcgg    >  2:219476/1‑93 (MQ=255)
                                                                                    aaaTCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgcgg    <  2:1888553/93‑1 (MQ=255)
                                                                                     aaTCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTgcggg   >  1:3457774/1‑93 (MQ=255)
                                                                                      aTCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGACAACCCAGCCAGGTATTGACGATAATTAGCATCGTgcgggg  >  1:2047344/1‑92 (MQ=255)
                                                                                          |                                                                                        
GTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGC  >  NC_000913/4241375‑4241553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTG  >  NC_000913/4241373‑4241557
                                                                                            |                                                                                            
GCGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCC                                                                                             <  SRR4302535.3655254/94‑1 (MQ=60)
 CGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCC                                                                                            >  SRR4302535.2276631/1‑94 (MQ=60)
    CAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGA                                                                                         >  SRR4302535.1689622/1‑94 (MQ=60)
     AGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAA                                                                                        >  SRR4302535.2991506/1‑94 (MQ=60)
     AGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAA                                                                                        >  SRR4302535.368023/1‑94 (MQ=60)
     AGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAA                                                                                        <  SRR4302535.3780263/94‑1 (MQ=60)
      GCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAAT                                                                                       <  SRR4302535.2832186/94‑1 (MQ=60)
      GCGGTTGAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAAT                                                                                       <  SRR4302535.1199502/94‑1 (MQ=60)
       CGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATC                                                                                      <  SRR4302535.770406/94‑1 (MQ=60)
       CGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATC                                                                                      <  SRR4302535.1700549/94‑1 (MQ=60)
       CCGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATC                                                                                      <  SRR4302535.16808/94‑1 (MQ=60)
        GGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCG                                                                                     >  SRR4302535.3421260/1‑94 (MQ=60)
        GGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCG                                                                                     >  SRR4302535.4559156/1‑94 (MQ=60)
          TTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCT                                                                                   <  SRR4302535.4221246/94‑1 (MQ=60)
           TTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTT                                                                                  <  SRR4302535.4151273/94‑1 (MQ=60)
           TTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTT                                                                                  >  SRR4302535.3364846/1‑94 (MQ=60)
             AATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTC                                                                                <  SRR4302535.3913461/94‑1 (MQ=60)
             AATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTC                                                                                >  SRR4302535.3171441/1‑94 (MQ=60)
              ATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCA                                                                               <  SRR4302535.1759009/94‑1 (MQ=60)
               TCAGCAGCGGCAGCGTAATCTTAAAGAACTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAG                                                                              <  SRR4302535.1249152/94‑1 (MQ=60)
                CAGCTGCGGCAGCGTAATCTTAAAGAAGTTCTGGACCGGCCCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGC                                                                             <  SRR4302535.266175/94‑1 (MQ=60)
                 AGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCA                                                                            <  SRR4302535.2922944/94‑1 (MQ=60)
                   CAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAG                                                                          <  SRR4302535.2911149/94‑1 (MQ=60)
                    AGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGC                                                                         >  SRR4302535.1753866/1‑94 (MQ=60)
                    AGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGC                                                                         >  SRR4302535.1431047/1‑94 (MQ=60)
                     GCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCC                                                                        <  SRR4302535.444750/94‑1 (MQ=60)
                      CGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCC                                                                       <  SRR4302535.3721765/94‑1 (MQ=60)
                        GCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGCGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCAT                                                                     >  SRR4302535.79632/1‑94 (MQ=60)
                         CAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATG                                                                    <  SRR4302535.697216/94‑1 (MQ=60)
                          AGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGC                                                                   >  SRR4302535.4201303/1‑94 (MQ=60)
                            CGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAG                                                                 <  SRR4302535.3207653/94‑1 (MQ=60)
                             GTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGA                                                                >  SRR4302535.3091235/1‑94 (MQ=60)
                             GTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGA                                                                >  SRR4302535.4551643/1‑94 (MQ=60)
                              TAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAG                                                               <  SRR4302535.1639951/94‑1 (MQ=60)
                              TAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAG                                                               <  SRR4302535.1607367/94‑1 (MQ=60)
                              TAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAG                                                               <  SRR4302535.4374166/94‑1 (MQ=60)
                               AATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGG                                                              <  SRR4302535.2520416/94‑1 (MQ=60)
                                ATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGA                                                             >  SRR4302535.1194230/1‑94 (MQ=60)
                                 TCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGAT                                                            <  SRR4302535.1823259/94‑1 (MQ=60)
                                    TAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCAT                                                         <  SRR4302535.1020863/94‑1 (MQ=60)
                                     AAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATC                                                        >  SRR4302535.855666/1‑94 (MQ=60)
                                     AAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATC                                                        >  SRR4302535.2325912/1‑94 (MQ=60)
                                     AAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATC                                                        >  SRR4302535.2939469/1‑94 (MQ=60)
                                      AAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCA                                                       <  SRR4302535.876907/94‑1 (MQ=60)
                                      AAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCA                                                       <  SRR4302535.1127717/94‑1 (MQ=60)
                                      AAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCACGCAGAGGATCATCA                                                       >  SRR4302535.785361/1‑94 (MQ=60)
                                       AGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCAT                                                      >  SRR4302535.2117544/1‑94 (MQ=60)
                                        GAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATG                                                     <  SRR4302535.139173/94‑1 (MQ=60)
                                          AGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTA                                                   <  SRR4302535.755380/94‑1 (MQ=60)
                                           GTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTAC                                                  <  SRR4302535.616668/94‑1 (MQ=60)
                                            TTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACG                                                 <  SRR4302535.539537/94‑1 (MQ=60)
                                             TCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGG                                                <  SRR4302535.108573/94‑1 (MQ=60)
                                               TGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAT                                              >  SRR4302535.4593973/1‑94 (MQ=60)
                                                  AACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAAC                                           <  SRR4302535.2454429/94‑1 (MQ=60)
                                                    CGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCC                                         >  SRR4302535.195660/1‑94 (MQ=60)
                                                     GGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCA                                        >  SRR4302535.2600135/1‑94 (MQ=60)
                                                     GGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCA                                        >  SRR4302535.1879312/1‑94 (MQ=60)
                                                      GACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAG                                       <  SRR4302535.3929356/94‑1 (MQ=60)
                                                      GACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAG                                       <  SRR4302535.3259370/94‑1 (MQ=60)
                                                       ACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGC                                      >  SRR4302535.1890212/1‑94 (MQ=60)
                                                        CCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCC                                     >  SRR4302535.1395695/1‑94 (MQ=60)
                                                        CCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCC                                     <  SRR4302535.4444974/94‑1 (MQ=60)
                                                        CCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCCTTCAGCAAGCCCATGCAGCGGATCATCATGTACGGATAACCCAGCC                                     >  SRR4302535.780658/1‑94 (MQ=60)
                                                         CTGCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAATCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCA                                    <  SRR4302535.2114365/94‑1 (MQ=60)
                                                           GCGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGG                                  <  SRR4302535.2337769/94‑1 (MQ=60)
                                                            CGCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGT                                 >  SRR4302535.2155919/1‑94 (MQ=60)
                                                             GCCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTA                                >  SRR4302535.4665985/1‑94 (MQ=60)
                                                              CCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAT                               <  SRR4302535.2239748/94‑1 (MQ=60)
                                                              CCATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTAT                               >  SRR4302535.4312195/1‑94 (MQ=60)
                                                               CATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT                              >  SRR4302535.2071878/1‑94 (MQ=60)
                                                               CATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT                              >  SRR4302535.4544359/1‑94 (MQ=60)
                                                               CATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT                              >  SRR4302535.2945511/1‑94 (MQ=60)
                                                               CATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT                              >  SRR4302535.2071877/1‑94 (MQ=60)
                                                               CATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATT                              >  SRR4302535.2910156/1‑94 (MQ=60)
                                                                ATCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTG                             >  SRR4302535.2572936/1‑94 (MQ=60)
                                                                 TCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGA                            <  SRR4302535.3385109/94‑1 (MQ=60)
                                                                 TCCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGA                            <  SRR4302535.1805348/94‑1 (MQ=60)
                                                                  CCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGAC                           <  SRR4302535.1353005/94‑1 (MQ=60)
                                                                  CCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGAC                           >  SRR4302535.2945093/1‑94 (MQ=60)
                                                                  CCATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGAC                           <  SRR4302535.3310981/94‑1 (MQ=60)
                                                                   CATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACG                          >  SRR4302535.1828919/1‑94 (MQ=60)
                                                                    ATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGA                         <  SRR4302535.2503701/94‑1 (MQ=60)
                                                                    ATTGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGAAGA                         >  SRR4302535.3889645/1‑94 (MQ=60)
                                                                      TGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATA                       >  SRR4302535.31146/1‑94 (MQ=60)
                                                                      TGCTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATA                       <  SRR4302535.4380343/94‑1 (MQ=60)
                                                                        CTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAAT                     >  SRR4302535.466784/1‑94 (MQ=60)
                                                                        CTGAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAAT                     <  SRR4302535.1546504/94‑1 (MQ=60)
                                                                          GAGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTA                   <  SRR4302535.3993479/94‑1 (MQ=60)
                                                                           AGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAG                  <  SRR4302535.1363519/94‑1 (MQ=60)
                                                                           AGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAG                  <  SRR4302535.285305/94‑1 (MQ=60)
                                                                           AGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAG                  >  SRR4302535.119221/1‑94 (MQ=60)
                                                                           AGGCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAG                  >  SRR4302535.238187/1‑94 (MQ=60)
                                                                             GCTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCA                <  SRR4302535.2707589/94‑1 (MQ=60)
                                                                              CTTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCAT               <  SRR4302535.4573410/94‑1 (MQ=60)
                                                                               TTCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATC              <  SRR4302535.843515/94‑1 (MQ=60)
                                                                                TCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCG             <  SRR4302535.1499752/94‑1 (MQ=60)
                                                                                TCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCG             <  SRR4302535.1824574/94‑1 (MQ=60)
                                                                                TCATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCG             <  SRR4302535.2011354/94‑1 (MQ=60)
                                                                                 CATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGT            >  SRR4302535.547133/1‑94 (MQ=60)
                                                                                  ATACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTG           >  SRR4302535.1285797/1‑94 (MQ=60)
                                                                                   TACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGC          >  SRR4302535.2149196/1‑94 (MQ=60)
                                                                                    ACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCG         <  SRR4302535.2912817/94‑1 (MQ=60)
                                                                                    ACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCG         <  SRR4302535.4118987/94‑1 (MQ=60)
                                                                                    ACAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCG         <  SRR4302535.2225327/94‑1 (MQ=60)
                                                                                     CAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGG        <  SRR4302535.305697/94‑1 (MQ=60)
                                                                                     CAAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGG        <  SRR4302535.939745/94‑1 (MQ=60)
                                                                                      AAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGG       >  SRR4302535.4462567/1‑94 (MQ=60)
                                                                                      AAATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGG       >  SRR4302535.3481814/1‑94 (MQ=60)
                                                                                       AATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGACAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGG      >  SRR4302535.2062085/1‑94 (MQ=60)
                                                                                        ATCGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCG     <  SRR4302535.2235586/94‑1 (MQ=60)
                                                                                          CGCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGT   <  SRR4302535.1224389/94‑1 (MQ=60)
                                                                                           GCCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTG  >  SRR4302535.4389976/1‑94 (MQ=60)
                                                                                            |                                                                                            
GCGTCAGCGGTTTAATCAGCAGCGGCAGCGTAATCTTAAAGAAGTTCTGGAACGGACCTGCGCCATCCATTGCTGAGGCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTG  >  NC_000913/4241373‑4241557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑