Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,242,957 C→T E348K (GAA→AAA)  malE ← periplasmic maltose‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,9570CT99.5% 1432.1 / ‑6.1 393E348K (GAA→AAA) malEperiplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (175/216);  total (175/218)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046
                                                                                          |                                                                                         
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  2:2450524/93‑1 (MQ=255)
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         >  1:5291183/1‑93 (MQ=255)
 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  1:6680078/93‑1 (MQ=255)
  gcggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtg                                                                                       <  1:7906156/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:5189220/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:3825215/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  2:3117613/93‑1 (MQ=255)
       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtggg                                                                                  >  1:195100/1‑92 (MQ=255)
       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggc                                                                                  <  1:6143308/93‑1 (MQ=255)
        ttGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcg                                                                                 >  1:3258495/1‑93 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  2:5649509/93‑1 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             >  1:1472383/1‑93 (MQ=255)
            tCACCAAAGTGCGCAAGGCGTACCAGAAAGCGGACAACTAAGGGCTGTTCGGCATGATTACACCTTTCTGGACGTTTTTCGTGGTGACGGCaa                                                                             <  1:6034683/93‑1 (MQ=255)
              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATTATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           >  2:5881689/1‑93 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:4111394/93‑1 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:7306648/93‑1 (MQ=255)
                  cAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGGGGCGGCAATACGTg                                                                       >  1:887975/1‑93 (MQ=255)
                   aGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTgg                                                                      >  2:6057274/1‑93 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:4064238/93‑1 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:4756557/93‑1 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     >  1:2402964/1‑93 (MQ=255)
                     tACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAt                                                                    <  2:1250952/93‑1 (MQ=255)
                      aCGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATc                                                                   >  1:25800/1‑93 (MQ=255)
                       cGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCt                                                                  <  2:3959238/93‑1 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACCTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGGAATACGTGGATCtt                                                                 >  1:6211437/1‑93 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 >  1:6868217/1‑93 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 <  2:6944283/93‑1 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 <  2:7893510/93‑1 (MQ=255)
                         cACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCttt                                                                <  1:6735818/93‑1 (MQ=255)
                          aCGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  1:1045649/93‑1 (MQ=255)
                          aCGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  1:2475842/93‑1 (MQ=255)
                          aCGACATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  2:2751494/93‑1 (MQ=255)
                             gCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                            >  2:5252431/1‑93 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:1940219/1‑93 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:4835245/1‑93 (MQ=255)
                                  ccAGAAAGCGGCCATCTGGGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAGCTTTCGCCAGctc                                                       <  2:1558410/93‑1 (MQ=255)
                                  ccAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       <  1:6515536/93‑1 (MQ=255)
                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  2:5570701/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:4580394/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    <  2:2537974/93‑1 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  2:2925511/1‑93 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:100625/93‑1 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:6816552/93‑1 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:7812999/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:4189691/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:779795/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  2:338884/93‑1 (MQ=255)
                                       aaGCGGACATCGGCGGGATGTTCGGCATGATTGCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  2:1715384/93‑1 (MQ=255)
                                        aGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTc                                                 <  1:1737782/93‑1 (MQ=255)
                                        aGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTc                                                 <  2:3465183/93‑1 (MQ=255)
                                          cGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                               <  2:3322034/93‑1 (MQ=255)
                                           ggACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTa                                              >  2:1821116/1‑93 (MQ=255)
                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  2:4502454/93‑1 (MQ=255)
                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  1:5556317/93‑1 (MQ=255)
                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAg                                            <  2:7933400/93‑1 (MQ=255)
                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAg                                            >  1:2232767/1‑93 (MQ=255)
                                              cATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAgc                                           >  1:7597679/1‑92 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  2:3082734/1‑93 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  2:5072254/1‑93 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         <  2:3880802/93‑1 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  1:2293401/93‑1 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      >  1:1797253/1‑93 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  2:7764440/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  2:1035474/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  1:727112/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     >  1:5726228/1‑93 (MQ=255)
                                                       gATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcgc                                  >  2:5391497/1‑93 (MQ=255)
                                                         tGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTa                                <  1:1221938/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:4758419/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:5928663/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:3505326/93‑1 (MQ=255)
                                                            tCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACgg                             >  1:1850499/1‑93 (MQ=255)
                                                                cATGATTTCACATTTCTGGGTGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAcc                         <  2:1405885/93‑1 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:3522370/1‑93 (MQ=255)
                                                                  tGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCa                       >  2:2277196/1‑93 (MQ=255)
                                                                   gATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                      >  2:6206691/1‑93 (MQ=255)
                                                                     tttCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCg                    <  2:7363202/93‑1 (MQ=255)
                                                                       tccccTTTCTGGGCCTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:6778641/90‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  >  1:3452571/1‑93 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:144438/93‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:5795286/93‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:5983206/93‑1 (MQ=255)
                                                                         tccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:704898/92‑1 (MQ=255)
                                                                         cccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:7233811/92‑1 (MQ=255)
                                                                         cccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  1:6372375/92‑1 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                >  2:7463988/1‑93 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:4643084/93‑1 (MQ=255)
                                                                          ccTTTCTGGGCGTTTTTCATTGTGGCGGCAATACGCGGATCTTTCGTCAACTCTTCCTCGTAAGACTTCAGCGCTACTTCACCCATCGGATTg               >  2:647779/1‑93 (MQ=255)
                                                                          ccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTg               <  1:4943705/93‑1 (MQ=255)
                                                                           cTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt              <  1:214073/93‑1 (MQ=255)
                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:2109071/1‑93 (MQ=255)
                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         >  2:6696614/1‑93 (MQ=255)
                                                                                 gggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        >  1:6996474/1‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  1:1612219/93‑1 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  1:742620/1‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  1:2981059/93‑1 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:4372101/1‑93 (MQ=255)
                                                                                    cGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTaa     <  1:2744492/93‑1 (MQ=255)
                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:3679043/1‑93 (MQ=255)
                                                                                      ttttTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc   >  1:5422448/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  >  NC_000913/4242864‑4243050
                                                                                             |                                                                                             
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTT                                                                                               <  SRR4302543.6557430/94‑1 (MQ=60)
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTT                                                                                               >  SRR4302543.7638611/1‑94 (MQ=60)
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTT                                                                                               >  SRR4302543.2150455/1‑94 (MQ=60)
 GCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTC                                                                                              <  SRR4302543.2701485/94‑1 (MQ=60)
  CTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCA                                                                                             >  SRR4302543.5338959/1‑94 (MQ=60)
  CTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCA                                                                                             <  SRR4302543.4888388/94‑1 (MQ=60)
   TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAT                                                                                            >  SRR4302543.2562854/1‑94 (MQ=60)
   TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAT                                                                                            <  SRR4302543.1874563/94‑1 (MQ=60)
    GGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATG                                                                                           <  SRR4302543.6743145/94‑1 (MQ=60)
    GGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATG                                                                                           <  SRR4302543.1737952/94‑1 (MQ=60)
     tgttttttttttttcaagcagaagACGGCATACGAGATAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGG                                                                                          <  SRR4302543.2794897/70‑1 (MQ=60)
     GCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGG                                                                                          <  SRR4302543.7980450/94‑1 (MQ=60)
     GCGGCGTTGATCACCAAAGAACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGG                                                                                          <  SRR4302543.2260615/94‑1 (MQ=60)
      GGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGT                                                                                         <  SRR4302543.2286253/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302543.3858957/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302543.5236024/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        >  SRR4302543.993255/1‑94 (MQ=60)
        GCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGG                                                                                       >  SRR4302543.1829225/1‑94 (MQ=60)
         gcgttaatccccgcagtacccagggattccaagaagggggccTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGC                                                                                      <  SRR4302543.63995/52‑1 (MQ=60)
         CGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGG                                                                                      >  SRR4302543.196747/1‑94 (MQ=60)
         CGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGC                                                                                      >  SRR4302543.453762/1‑94 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     >  SRR4302543.3287947/1‑94 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     <  SRR4302543.7060276/94‑1 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     <  SRR4302543.6201491/94‑1 (MQ=60)
            TGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGC                                                                                   >  SRR4302543.2426849/1‑94 (MQ=60)
              ATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAA                                                                                 >  SRR4302543.1516887/1‑94 (MQ=60)
              ATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAA                                                                                 >  SRR4302543.1485646/1‑94 (MQ=60)
              ATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAA                                                                                 >  SRR4302543.1803307/1‑94 (MQ=60)
               TCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAAT                                                                                <  SRR4302543.259362/94‑1 (MQ=60)
                TACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCGCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATA                                                                               <  SRR4302543.4938594/94‑1 (MQ=60)
                CACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATA                                                                               <  SRR4302543.6204320/94‑1 (MQ=60)
                CACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATA                                                                               <  SRR4302543.7279390/94‑1 (MQ=60)
                CACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATA                                                                               <  SRR4302543.2352267/94‑1 (MQ=60)
                 ACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAC                                                                              <  SRR4302543.6624019/94‑1 (MQ=60)
                 ACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAC                                                                              <  SRR4302543.4966816/94‑1 (MQ=60)
                  CCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACG                                                                             <  SRR4302543.6768069/94‑1 (MQ=60)
                  CCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACG                                                                             <  SRR4302543.2097155/94‑1 (MQ=60)
                   caagcagaagACGGCATACGAGATAGCGGACATCTGCGGGATGTTCGGCATGATTTCCCCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGT                                                                            <  SRR4302543.1068188/84‑1 (MQ=60)
                    GCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGGGGCGGCAATACGTG                                                                           >  SRR4302543.895839/1‑94 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          <  SRR4302543.4001691/94‑1 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          >  SRR4302543.3119009/1‑94 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          <  SRR4302543.5779830/94‑1 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          <  SRR4302543.6117524/94‑1 (MQ=60)
                      AGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGA                                                                         >  SRR4302543.2424695/1‑94 (MQ=60)
                      AGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGA                                                                         <  SRR4302543.7936634/94‑1 (MQ=60)
                       gtgcgccgGGCATTCCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302543.4223772/86‑1 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302543.4100632/94‑1 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        >  SRR4302543.2947683/1‑94 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        >  SRR4302543.7494478/1‑94 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302543.7347859/94‑1 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302543.4800025/94‑1 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        >  SRR4302543.6631092/1‑94 (MQ=60)
                        TACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATC                                                                       >  SRR4302543.26030/1‑94 (MQ=60)
                         ACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCT                                                                      <  SRR4302543.3183867/94‑1 (MQ=60)
                         ACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCT                                                                      <  SRR4302543.421875/94‑1 (MQ=60)
                          CGCACGGCATACCAGAAAGCGGACCTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGGAATACGTGGATCTT                                                                     >  SRR4302543.6270088/1‑94 (MQ=60)
                          CGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTT                                                                     >  SRR4302543.6772441/1‑94 (MQ=60)
                          CGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTT                                                                     <  SRR4302543.4662586/94‑1 (MQ=60)
                          CGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTT                                                                     >  SRR4302543.6932907/1‑94 (MQ=60)
                          CGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTT                                                                     >  SRR4302543.6270087/1‑94 (MQ=60)
                           GCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTT                                                                    <  SRR4302543.2906450/94‑1 (MQ=60)
                            CACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTC                                                                   <  SRR4302543.6799443/94‑1 (MQ=60)
                            CACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTC                                                                   >  SRR4302543.473026/1‑94 (MQ=60)
                            CACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTC                                                                   >  SRR4302543.7524806/1‑94 (MQ=60)
                            CACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTC                                                                   <  SRR4302543.2164569/94‑1 (MQ=60)
                             ACGGCATACGAGAAAGCGGACATCTGCTGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCG                                                                  <  SRR4302543.1764015/94‑1 (MQ=60)
                             ACGGCATACCAGAAATCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCG                                                                  >  SRR4302543.7859413/1‑94 (MQ=60)
                             ACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTGG                                                                  >  SRR4302543.6344623/1‑94 (MQ=60)
                             ACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCG                                                                  <  SRR4302543.2498230/94‑1 (MQ=60)
                             ACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCG                                                                  <  SRR4302543.1054998/94‑1 (MQ=60)
                               GGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCC                                                                <  SRR4302543.6505573/94‑1 (MQ=60)
                               GGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCC                                                                >  SRR4302543.7279865/1‑94 (MQ=60)
                                  ATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAC                                                             <  SRR4302543.5724263/94‑1 (MQ=60)
                                   TACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACT                                                            <  SRR4302543.7980249/94‑1 (MQ=60)
                                    ACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCAGCTTTCTGGGCGTTTTTCATGGTGGTGGCGTTACGTGGGTCTTTCGCCAAatt                                                           >  SRR4302543.3016084/1‑91 (MQ=60)
                                    ACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTC                                                           >  SRR4302543.5923528/1‑94 (MQ=60)
                                     CCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCT                                                          <  SRR4302543.2261910/94‑1 (MQ=60)
                                     CCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCT                                                          <  SRR4302543.6576902/94‑1 (MQ=60)
                                      CAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTT                                                         >  SRR4302543.888440/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302543.2160073/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302543.4622019/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGCGGAGTTTGCGCCAACTCTTC                                                        >  SRR4302543.236975/1‑94 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302543.786647/94‑1 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302543.786648/94‑1 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302543.3446898/94‑1 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302543.4227677/94‑1 (MQ=60)
                                           AGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCG                                                    <  SRR4302543.1753277/94‑1 (MQ=60)
                                           AGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCG                                                    >  SRR4302543.5967313/1‑94 (MQ=60)
                                              GGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAA                                                 >  SRR4302543.5071569/1‑94 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                >  SRR4302543.2252959/1‑94 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302543.5606850/94‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302543.1080848/94‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302543.6176813/94‑1 (MQ=60)
                                                ACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGC                                               >  SRR4302543.7669841/1‑94 (MQ=60)
                                                ACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGA                                               >  SRR4302543.4434455/1‑94 (MQ=60)
                                                ACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGA                                               <  SRR4302543.1853094/94‑1 (MQ=60)
                                                  ATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACT                                             <  SRR4302543.7349782/94‑1 (MQ=60)
                                                  ATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACT                                             <  SRR4302543.6752134/94‑1 (MQ=60)
                                                  ATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACT                                             >  SRR4302543.2762746/1‑94 (MQ=60)
                                                    CTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTC                                           >  SRR4302543.2953427/1‑94 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          <  SRR4302543.5350185/94‑1 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          <  SRR4302543.5350184/94‑1 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          <  SRR4302543.1210463/94‑1 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          >  SRR4302543.1813343/1‑94 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          <  SRR4302543.2314323/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302543.1813325/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         >  SRR4302543.5778479/1‑94 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302543.2318791/94‑1 (MQ=60)
                                                       TGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTAGCCAACTCTTCCTCGTAAGACTTCAGC                                        >  SRR4302543.3546649/1‑94 (MQ=60)
                                                       CGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGC                                        <  SRR4302543.216481/94‑1 (MQ=60)
                                                       CGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGC                                        <  SRR4302543.733649/94‑1 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGTCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       <  SRR4302543.7041176/94‑1 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       <  SRR4302543.7670674/94‑1 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       <  SRR4302543.7524886/94‑1 (MQ=60)
                                                          GATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCT                                     <  SRR4302543.1864177/94‑1 (MQ=60)
                                                           GTGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.4174048/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.3128351/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.6680427/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.5404836/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.8006758/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302543.4174049/1‑94 (MQ=60)
                                                            TGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAC                                   <  SRR4302543.1232921/94‑1 (MQ=60)
                                                            TGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAC                                   <  SRR4302543.1381354/94‑1 (MQ=60)
                                                            TGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAC                                   <  SRR4302543.2538539/94‑1 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  <  SRR4302543.681085/94‑1 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  >  SRR4302543.1262632/1‑94 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  >  SRR4302543.5489779/1‑94 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  <  SRR4302543.2448977/94‑1 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCAAACTCTTCCTCGTAAGACTTCAGCCCTACG                                  >  SRR4302543.2482949/1‑94 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTGCTGGGCGGTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  <  SRR4302543.6526351/94‑1 (MQ=60)
                                                              TTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGG                                 >  SRR4302543.1867146/1‑94 (MQ=60)
                                                              TTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGG                                 <  SRR4302543.6472042/94‑1 (MQ=60)
                                                                  GCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACC                             >  SRR4302543.7668128/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302543.3484225/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302543.5777041/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302543.3553792/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCACCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302543.919762/1‑94 (MQ=60)
                                                                    ATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCA                           >  SRR4302543.6865999/1‑94 (MQ=60)
                                                                    ATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCA                           >  SRR4302543.3382365/1‑94 (MQ=60)
                                                                    ATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCA                           >  SRR4302543.6990698/1‑94 (MQ=60)
                                                                      GATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGC                         >  SRR4302543.4713598/1‑94 (MQ=60)
                                                                       ATTTCGCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        <  SRR4302543.1540447/94‑1 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        >  SRR4302543.1345874/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        >  SRR4302543.5330693/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        <  SRR4302543.1948399/94‑1 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        >  SRR4302543.5737119/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        >  SRR4302543.6258374/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        <  SRR4302543.3860106/94‑1 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      >  SRR4302543.2712333/1‑94 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      >  SRR4302543.3483487/1‑94 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      >  SRR4302543.5481073/1‑94 (MQ=60)
                                                                          TCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTT                     >  SRR4302543.3443630/1‑94 (MQ=60)
                                                                          TCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTT                     >  SRR4302543.1508383/1‑94 (MQ=60)
                                                                           CACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTT                    >  SRR4302543.4866017/1‑94 (MQ=60)
                                                                           CACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTT                    >  SRR4302543.2130030/1‑94 (MQ=60)
                                                                            CCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTG                   <  SRR4302543.6432317/94‑1 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGT                  <  SRR4302543.4988721/94‑1 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGT                  >  SRR4302543.3840534/1‑94 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGT                  <  SRR4302543.2687070/94‑1 (MQ=60)
                                                                              CTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTC                 <  SRR4302543.215903/94‑1 (MQ=60)
                                                                               TTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCT                >  SRR4302543.1693725/1‑94 (MQ=60)
                                                                                 TCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTT              <  SRR4302543.5288158/94‑1 (MQ=60)
                                                                                 TCGGGGCGTTTTTCATGGGGGCGGCAAAACGTTGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTT              <  SRR4302543.6305920/94‑1 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTT             >  SRR4302543.3679020/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302543.924308/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302543.2128369/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302543.1873444/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302543.3977198/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302543.5008127/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            >  SRR4302543.7062517/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            >  SRR4302543.5300281/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302543.7308881/94‑1 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            >  SRR4302543.86463/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302543.4893695/94‑1 (MQ=60)
                                                                                    GGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATT           >  SRR4302543.749241/1‑94 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          <  SRR4302543.5085269/94‑1 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          <  SRR4302543.3008130/94‑1 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          >  SRR4302543.3851841/1‑94 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          <  SRR4302543.1626600/94‑1 (MQ=60)
                                                                                      GCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAA         <  SRR4302543.6104698/94‑1 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        <  SRR4302543.4540945/94‑1 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        <  SRR4302543.3485483/94‑1 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        <  SRR4302543.2769442/94‑1 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        <  SRR4302543.7704812/94‑1 (MQ=60)
                                                                                        GTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACC       >  SRR4302543.5471744/1‑94 (MQ=60)
                                                                                        GTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACC       <  SRR4302543.1706152/94‑1 (MQ=60)
                                                                                        GTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACC       <  SRR4302543.6376893/94‑1 (MQ=60)
                                                                                        GTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACC       >  SRR4302543.6889231/1‑94 (MQ=60)
                                                                                          TTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGC     <  SRR4302543.5551340/94‑1 (MQ=60)
                                                                                          TTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGC     >  SRR4302543.4727600/1‑94 (MQ=60)
                                                                                          TTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGC     >  SRR4302543.4438724/1‑94 (MQ=60)
                                                                                           TTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCT    <  SRR4302543.294729/94‑1 (MQ=60)
                                                                                           TTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCT    >  SRR4302543.6606886/1‑94 (MQ=60)
                                                                                            TTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTT   <  SRR4302543.5585967/94‑1 (MQ=60)
                                                                                            TTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTT   >  SRR4302543.6286179/1‑94 (MQ=60)
                                                                                            TTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTT   >  SRR4302543.393223/1‑94 (MQ=60)
                                                                                             TCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  >  SRR4302543.262112/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  >  NC_000913/4242864‑4243050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑