Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,243,337	A→G	L221P (CTG→CCG)	malE ←	periplasmic maltose‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,243,337	0	A	G	99.7%	1187.6 / ‑5.2	371	L221P (CTG→CCG)	malE	periplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (182/188); total (183/188)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 378 total aligned reads displayed.

GTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC  >  NC_000913/4243247‑4243426

gTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCggg                                                                                         >  2:2723/1‑93 (MQ=255)

gTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCggg                                                                                         >  1:2972058/1‑93 (MQ=255)

 tCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGa                                                                                        >  2:7248123/1‑93 (MQ=255)

 tCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTAAACCGGGa                                                                                        >  2:5727647/1‑93 (MQ=255)

   aTCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAg                                                                                      >  1:1119032/1‑93 (MQ=255)

   aTCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAg                                                                                      >  2:1171749/1‑93 (MQ=255)

   aTCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAg                                                                                      >  1:4677801/1‑93 (MQ=255)

    tCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAgg                                                                                     >  2:3905985/1‑93 (MQ=255)

    tCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAgg                                                                                     >  2:5227818/1‑93 (MQ=255)

      gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   <  2:2565227/93‑1 (MQ=255)

      gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   >  1:7205782/1‑93 (MQ=255)

       cTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCa                                                                                  >  2:7697771/1‑93 (MQ=255)

       cTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCa                                                                                  <  1:5503328/93‑1 (MQ=255)

       cTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCa                                                                                  <  1:4425269/93‑1 (MQ=255)

        tGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAg                                                                                 >  2:5796827/1‑93 (MQ=255)

        tGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAg                                                                                 >  1:6827946/1‑93 (MQ=255)

         gTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGa                                                                                >  1:1825010/1‑93 (MQ=255)

          tttCGCCTTTATTAAAGGGAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGAGTTTGTCTTTAATCAGGTCAACAGGGAAGGTCAGAc                                                                               >  1:41643/1‑93 (MQ=255)

          tttCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGAc                                                                               >  1:5285770/1‑93 (MQ=255)

           ttCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGAcc                                                                              <  2:5311130/93‑1 (MQ=255)

                cTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCtt                                                                         <  2:7337777/93‑1 (MQ=255)

                 tttATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCttt                                                                        <  2:7505035/93‑1 (MQ=255)

                 tttATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCttt                                                                        <  1:7295549/93‑1 (MQ=255)

                 tttATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCttt                                                                        >  1:7079776/1‑93 (MQ=255)

                 tttATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCttt                                                                        >  1:6720893/1‑93 (MQ=255)

                  ttattaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTc                                                                       <  1:7436241/93‑1 (MQ=255)

                   tattaAAGGCAGCTTCTGCTATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcg                                                                      >  1:92184/1‑93 (MQ=255)

                   tattaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcg                                                                      >  1:86966/1‑93 (MQ=255)

                   tattaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcg                                                                      <  1:5784209/93‑1 (MQ=255)

                     ttaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcgcg                                                                    >  2:1002805/1‑93 (MQ=255)

                     ttaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcgcg                                                                    >  1:3138777/1‑93 (MQ=255)

                         aGGCAGCTTCTGCGATGGGGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACAGGGAAGGTCAGACACGCTTTCGCGCCAg                                                                >  2:6631258/1‑93 (MQ=255)

                           gCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCg                                                              >  1:3874250/1‑93 (MQ=255)

                             aGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGtt                                                            >  1:3540853/1‑93 (MQ=255)

                              gCTTCTGCGATGGAGTATTCGGTGTCTACATTCATGTGTTTGTTTTTCATCAAATCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGATa                                                           <  2:7015894/93‑1 (MQ=255)

                              gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           <  2:2122656/93‑1 (MQ=255)

                              gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           >  1:3689982/1‑93 (MQ=255)

                              gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           >  2:6465761/1‑93 (MQ=255)

                              gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           <  1:1721546/93‑1 (MQ=255)

                              gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           <  1:5665554/93‑1 (MQ=255)

                              acTTCTGGGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           <  1:5397644/92‑1 (MQ=255)

                               cTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAt                                                          <  2:5582732/93‑1 (MQ=255)

                                ttCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATc                                                         >  1:6509157/1‑93 (MQ=255)

                                  cTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCa                                                       <  1:6302723/93‑1 (MQ=255)

                                   tGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAc                                                      <  2:3380513/93‑1 (MQ=255)

                                    gCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACg                                                     <  2:5938526/93‑1 (MQ=255)

                                     cGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGc                                                    <  1:2635935/93‑1 (MQ=255)

                                     cGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGc                                                    <  1:1285723/93‑1 (MQ=255)

                                      gATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGcc                                                   >  1:4064777/1‑93 (MQ=255)

                                         ggAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAc                                                <  1:6115869/93‑1 (MQ=255)

                                         ggAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAc                                                >  1:2432082/1‑93 (MQ=255)

                                            gTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTc                                             >  2:2236041/1‑93 (MQ=255)

                                                tCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTa                                         >  1:4316089/1‑93 (MQ=255)

                                                tCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTa                                         >  1:2125708/1‑93 (MQ=255)

                                                tCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGGCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTa                                         >  1:2291067/1‑93 (MQ=255)

                                                 cGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTaa                                        <  1:841282/93‑1 (MQ=255)

                                                 cGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTaa                                        <  2:6817652/93‑1 (MQ=255)

                                                   gtgtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATg                                      <  1:6199909/93‑1 (MQ=255)

                                                    tgtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGt                                     >  2:1710558/1‑93 (MQ=255)

                                                     gtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTc                                    <  1:7890060/93‑1 (MQ=255)

                                                     gtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTc                                    <  2:4596523/93‑1 (MQ=255)

                                                      tCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCg                                   >  1:2841871/1‑93 (MQ=255)

                                                      tCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCg                                   >  2:4301627/1‑93 (MQ=255)

                                                         tcATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                <  1:4535007/92‑1 (MQ=255)

                                                         gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                <  2:6326728/93‑1 (MQ=255)

                                                         gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                <  2:4398848/93‑1 (MQ=255)

                                                          cATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACt                               <  1:514231/93‑1 (MQ=255)

                                                           cttCATTTGTTTTTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCCCTTTCCCGCCAGCGTTATCCACGCCCACGTCCTTAATGTCGTACtt                              <  2:778976/92‑1 (MQ=255)

                                                            ttCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCTACGCCCACGTCTTTAATGTCGTACTTg                             >  1:3288718/1‑93 (MQ=255)

                                                            ttCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTg                             <  2:917999/93‑1 (MQ=255)

                                                            ttCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTg                             >  1:7698526/1‑93 (MQ=255)

                                                            ttCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTg                             >  2:4046019/1‑93 (MQ=255)

                                                             tCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGc                            <  1:4890109/93‑1 (MQ=255)

                                                             tCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGc                            <  2:2446697/93‑1 (MQ=255)

                                                              cATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGcc                           >  2:84356/1‑93 (MQ=255)

                                                                 gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGTGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        >  2:7119405/1‑93 (MQ=255)

                                                                 gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        >  1:4956102/1‑93 (MQ=255)

                                                                 gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        >  1:34669/1‑93 (MQ=255)

                                                                   gtttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtttt                      <  1:1596083/93‑1 (MQ=255)

                                                                    tttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTc                     >  2:5511456/1‑93 (MQ=255)

                                                                    tttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTc                     <  2:1238699/93‑1 (MQ=255)

                                                                     ttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCATACTTGCCGTTTTCa                    >  2:3776136/1‑93 (MQ=255)

                                                                      tgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCAt                   <  1:1412524/93‑1 (MQ=255)

                                                                        tttttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATAc                 <  2:5191928/93‑1 (MQ=255)

                                                                         ttttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACt                <  2:532996/93‑1 (MQ=255)

                                                                         ttttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACt                >  2:1433251/1‑93 (MQ=255)

                                                                           ttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTg              <  1:4784250/93‑1 (MQ=255)

                                                                            tAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGa             <  2:481353/93‑1 (MQ=255)

                                                                             aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGac            >  2:4993213/1‑92 (MQ=255)

                                                                             aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            >  2:3115512/1‑93 (MQ=255)

                                                                             aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCAACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            >  2:35783/1‑93 (MQ=255)

                                                                              aTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAAc           <  1:16975/93‑1 (MQ=255)

                                                                              aTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAAc           >  2:1866981/1‑93 (MQ=255)

                                                                               tCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACg          >  1:7173801/1‑93 (MQ=255)

                                                                               tCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTCTCATACTTGAACg          >  1:1089180/1‑93 (MQ=255)

                                                                                 aGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCa        >  2:4698026/1‑93 (MQ=255)

                                                                                   gTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATa      <  2:5457896/93‑1 (MQ=255)

                                                                                    tCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATac     >  1:2522806/1‑92 (MQ=255)

                                                                                    tCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATaa     <  2:6949367/93‑1 (MQ=255)

                                                                                      aaCCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAAcc   >  1:5927988/1‑93 (MQ=255)

GTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC  >  NC_000913/4243247‑4243426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 216 total aligned reads displayed.

ATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  >  NC_000913/4243244‑4243430

ATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCG                                                                                               >  SRR4302543.3140490/1‑94 (MQ=60)

ATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCG                                                                                               >  SRR4302543.1980915/1‑94 (MQ=60)

ATGGTCATCGCTGTTTCGCCTTTATTAAAGCCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCG                                                                                               >  SRR4302543.4962217/1‑94 (MQ=60)

 TGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGG                                                                                              >  SRR4302543.5763570/1‑94 (MQ=60)

  GGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGG                                                                                             >  SRR4302543.2999045/1‑94 (MQ=60)

   GTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGA                                                                                            >  SRR4302543.1962167/1‑94 (MQ=60)

    TCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAA                                                                                           >  SRR4302543.6252719/1‑94 (MQ=60)

     CATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAG                                                                                          <  SRR4302543.5536845/94‑1 (MQ=60)

     CATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAG                                                                                          >  SRR4302543.5761580/1‑94 (MQ=60)

     CATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAG                                                                                          >  SRR4302543.1129170/1‑94 (MQ=60)

     CATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAG                                                                                          >  SRR4302543.4720447/1‑94 (MQ=60)

      ATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGG                                                                                         >  SRR4302543.5978955/1‑94 (MQ=60)

      ATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGG                                                                                         >  SRR4302543.44266/1‑94 (MQ=60)

      ATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGG                                                                                         >  SRR4302543.7308348/1‑94 (MQ=60)

        CGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTC                                                                                       >  SRR4302543.7605615/1‑94 (MQ=60)

        CGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTC                                                                                       >  SRR4302543.301769/1‑94 (MQ=60)

        CGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTC                                                                                       >  SRR4302543.7275638/1‑94 (MQ=60)

         GCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCA                                                                                      <  SRR4302543.921592/94‑1 (MQ=60)

          CTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAG                                                                                     <  SRR4302543.5553283/94‑1 (MQ=60)

          CTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAG                                                                                     <  SRR4302543.4465368/94‑1 (MQ=60)

          CTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAG                                                                                     >  SRR4302543.6892406/1‑94 (MQ=60)

           TGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGA                                                                                    >  SRR4302543.521854/1‑94 (MQ=60)

            GTTTCGCCTTTATTAAAGGGAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGAGTTTGTCTTTAATCAGGTCAACAGGGAAGGTCAGAC                                                                                   >  SRR4302543.42024/1‑94 (MQ=60)

            GTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGAC                                                                                   <  SRR4302543.4144059/94‑1 (MQ=60)

            GTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGAC                                                                                   >  SRR4302543.5333479/1‑94 (MQ=60)

            GTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGAC                                                                                   <  SRR4302543.3725453/94‑1 (MQ=60)

             TTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACC                                                                                  >  SRR4302543.1727007/1‑94 (MQ=60)

             TTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACC                                                                                  <  SRR4302543.3858634/94‑1 (MQ=60)

              TTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCC                                                                                 <  SRR4302543.7206889/94‑1 (MQ=60)

              TTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCC                                                                                 <  SRR4302543.5818070/94‑1 (MQ=60)

              TTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCC                                                                                 <  SRR4302543.1422148/94‑1 (MQ=60)

              TTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCC                                                                                 <  SRR4302543.5257820/94‑1 (MQ=60)

                CGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGC                                                                               >  SRR4302543.3532914/1‑94 (MQ=60)

                 GCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCT                                                                              <  SRR4302543.6247244/94‑1 (MQ=60)

                 GCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCT                                                                              <  SRR4302543.865279/94‑1 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTT                                                                            <  SRR4302543.6148810/94‑1 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTT                                                                            >  SRR4302543.7820741/1‑94 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTT                                                                            >  SRR4302543.4489640/1‑94 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTT                                                                            >  SRR4302543.6784415/1‑94 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTT                                                                            >  SRR4302543.1189642/1‑94 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTCTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCCTT                                                                            <  SRR4302543.6148811/94‑1 (MQ=60)

                   CTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTAATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTT                                                                            >  SRR4302543.7743414/1‑94 (MQ=60)

                    TTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTC                                                                           <  SRR4302543.5706577/94‑1 (MQ=60)

                    TTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTC                                                                           <  SRR4302543.7365944/94‑1 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCTATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          >  SRR4302543.93045/1‑94 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          >  SRR4302543.87767/1‑94 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          >  SRR4302543.1370123/1‑94 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          <  SRR4302543.7507527/94‑1 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          <  SRR4302543.5122384/94‑1 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          <  SRR4302543.7781654/94‑1 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          <  SRR4302543.4131620/94‑1 (MQ=60)

                     TTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCG                                                                          <  SRR4302543.3951716/94‑1 (MQ=60)

                      TATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGC                                                                         <  SRR4302543.5836843/94‑1 (MQ=60)

                      TATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGC                                                                         >  SRR4302543.2455705/1‑94 (MQ=60)

                       ATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCG                                                                        >  SRR4302543.3167008/1‑94 (MQ=60)

                          AAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCA                                                                     <  SRR4302543.5336202/94‑1 (MQ=60)

                          AAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCA                                                                     <  SRR4302543.2143659/94‑1 (MQ=60)

                          AAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCAA                                                                     <  SRR4302543.3535003/94‑1 (MQ=60)

                             GGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCG                                                                  >  SRR4302543.6216502/1‑94 (MQ=60)

                             GGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCG                                                                  >  SRR4302543.7314115/1‑94 (MQ=60)

                               CAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCGTGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTT                                                                <  SRR4302543.5492899/94‑1 (MQ=60)

                               CAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTT                                                                >  SRR4302543.3572322/1‑94 (MQ=60)

                               CAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTT                                                                <  SRR4302543.6334775/94‑1 (MQ=60)

                                AGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTA                                                               >  SRR4302543.3722414/1‑94 (MQ=60)

                                 GCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAT                                                              >  SRR4302543.3521299/1‑94 (MQ=60)

                                 GCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAT                                                              <  SRR4302543.5717239/94‑1 (MQ=60)

                                 GCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAT                                                              <  SRR4302543.1736903/94‑1 (MQ=60)

                                 GCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAT                                                              <  SRR4302543.585389/94‑1 (MQ=60)

                                 ACTTCTGGGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTAT                                                              <  SRR4302543.5446730/94‑1 (MQ=60)

                                  CTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATC                                                             >  SRR4302543.1625916/1‑94 (MQ=60)

                                  CTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATC                                                             >  SRR4302543.7210667/1‑94 (MQ=60)

                                  CTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATC                                                             <  SRR4302543.2603998/94‑1 (MQ=60)

                                  CTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATC                                                             >  SRR4302543.6570470/1‑94 (MQ=60)

                                   TTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCC                                                            <  SRR4302543.2285446/94‑1 (MQ=60)

                                   TTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCC                                                            <  SRR4302543.7834839/94‑1 (MQ=60)

                                     TTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          <  SRR4302543.6826278/94‑1 (MQ=60)

                                     CTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          >  SRR4302543.2442423/1‑94 (MQ=60)

                                     CTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          <  SRR4302543.6362021/94‑1 (MQ=60)

                                     CTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          <  SRR4302543.407314/94‑1 (MQ=60)

                                     CTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          <  SRR4302543.2645570/94‑1 (MQ=60)

                                     CTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAC                                                          <  SRR4302543.6454971/94‑1 (MQ=60)

                                        CGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCC                                                       >  SRR4302543.7261113/1‑94 (MQ=60)

                                        CGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCC                                                       >  SRR4302543.4101180/1‑94 (MQ=60)

                                        CGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCC                                                       <  SRR4302543.2659803/94‑1 (MQ=60)

                                        CGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCC                                                       <  SRR4302543.1297188/94‑1 (MQ=60)

                                         GATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCC                                                      >  SRR4302543.3082673/1‑94 (MQ=60)

                                          ATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCA                                                     >  SRR4302543.859949/1‑94 (MQ=60)

                                           TGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAC                                                    <  SRR4302543.2828684/94‑1 (MQ=60)

                                           TGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAC                                                    >  SRR4302543.2454029/1‑94 (MQ=60)

                                           TGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAC                                                    <  SRR4302543.7266195/94‑1 (MQ=60)

                                           TGGAGTAATCGGTGGCTGCATTCATGTGTTTGTGTGTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAC                                                    <  SRR4302543.2828685/94‑1 (MQ=60)

                                           TGGAGTAATCAGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAC                                                    <  SRR4302543.3805855/94‑1 (MQ=60)

                                            TGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACG                                                   <  SRR4302543.172752/94‑1 (MQ=60)

                                            GGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGCCCCGCTTTCGCGCCAGCGTTATCCACGCCCACG                                                   <  SRR4302543.4312252/94‑1 (MQ=60)

                                            GGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACG                                                   <  SRR4302543.6173804/94‑1 (MQ=60)

                                            GGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACG                                                   >  SRR4302543.1285078/1‑94 (MQ=60)

                                              AGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTC                                                 >  SRR4302543.457159/1‑94 (MQ=60)

                                              AGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTC                                                 >  SRR4302543.170732/1‑94 (MQ=60)

                                              AGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTC                                                 >  SRR4302543.5487041/1‑94 (MQ=60)

                                               GTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCT                                                <  SRR4302543.1452417/94‑1 (MQ=60)

                                                 TATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCACCCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTT                                              <  SRR4302543.4886103/94‑1 (MQ=60)

                                                  ATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTA                                             >  SRR4302543.3958279/1‑94 (MQ=60)

                                                  ATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTA                                             >  SRR4302543.2144738/1‑94 (MQ=60)

                                                  ATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTA                                             >  SRR4302543.4355292/1‑94 (MQ=60)

                                                  ATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTA                                             >  SRR4302543.6276725/1‑94 (MQ=60)

                                                  ATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGGCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTA                                             >  SRR4302543.2311977/1‑94 (MQ=60)

                                                   TCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAA                                            >  SRR4302543.1267644/1‑94 (MQ=60)

                                                   TCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAA                                            >  SRR4302543.5336684/1‑94 (MQ=60)

                                                   TCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAA                                            <  SRR4302543.4610177/94‑1 (MQ=60)

                                                    TGGTGTCTGCATTCATGTTTTTGTTTTTAAGCAAGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAAT                                           <  SRR4302543.5915805/94‑1 (MQ=60)

                                                    CGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAAT                                           <  SRR4302543.1823158/94‑1 (MQ=60)

                                                    CGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAAT                                           <  SRR4302543.4469063/94‑1 (MQ=60)

                                                    CGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAAT                                           >  SRR4302543.3271831/1‑94 (MQ=60)

                                                    CGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAAT                                           <  SRR4302543.848713/94‑1 (MQ=60)

                                                      GTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGT                                         >  SRR4302543.2669190/1‑94 (MQ=60)

                                                      GTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGT                                         <  SRR4302543.6258472/94‑1 (MQ=60)

                                                      GTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGT                                         >  SRR4302543.4397806/1‑94 (MQ=60)

                                                       TGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTC                                        <  SRR4302543.2623071/94‑1 (MQ=60)

                                                       TGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTC                                        >  SRR4302543.3119253/1‑94 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       <  SRR4302543.7964267/94‑1 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       >  SRR4302543.2867841/1‑94 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       <  SRR4302543.1834369/94‑1 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       >  SRR4302543.6453552/1‑94 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       >  SRR4302543.6788986/1‑94 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       >  SRR4302543.3638982/1‑94 (MQ=60)

                                                        GTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCG                                       <  SRR4302543.5160958/94‑1 (MQ=60)

                                                         TCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGT                                      >  SRR4302543.2895340/1‑94 (MQ=60)

                                                          CTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTA                                     <  SRR4302543.3337298/94‑1 (MQ=60)

                                                          CTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTA                                     <  SRR4302543.3337299/94‑1 (MQ=60)

                                                           TGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAC                                    <  SRR4302543.112162/94‑1 (MQ=60)

                                                            TCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACT                                   <  SRR4302543.4576292/94‑1 (MQ=60)

                                                            GCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACT                                   <  SRR4302543.805930/94‑1 (MQ=60)

                                                              ATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCTACGCCCACGTCTTTAATGTCGTACTTG                                 >  SRR4302543.3318397/1‑94 (MQ=60)

                                                              ATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTG                                 >  SRR4302543.7771392/1‑94 (MQ=60)

                                                              ATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTG                                 <  SRR4302543.6699960/94‑1 (MQ=60)

                                                              ATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTG                                 >  SRR4302543.5788272/1‑94 (MQ=60)

                                                              ATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTC                                 >  SRR4302543.6259828/1‑94 (MQ=60)

                                                               CTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGC                                >  SRR4302543.6632754/1‑94 (MQ=60)

                                                                TCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCC                               >  SRR4302543.5238747/1‑94 (MQ=60)

                                                                TCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCC                               <  SRR4302543.4934688/94‑1 (MQ=60)

                                                                TCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCC                               <  SRR4302543.7048589/94‑1 (MQ=60)

                                                                TCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCC                               <  SRR4302543.1605380/94‑1 (MQ=60)

                                                                 CATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCG                              <  SRR4302543.5612069/94‑1 (MQ=60)

                                                                 CATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCG                              >  SRR4302543.7585389/1‑94 (MQ=60)

                                                                  ATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTGTAATGTCGTACTTGCCGT                             >  SRR4302543.901634/1‑94 (MQ=60)

                                                                   TGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTT                            >  SRR4302543.5001228/1‑94 (MQ=60)

                                                                   TGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTT                            >  SRR4302543.34986/1‑94 (MQ=60)

                                                                     TGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTT                          <  SRR4302543.5087617/94‑1 (MQ=60)

                                                                     TGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTT                          >  SRR4302543.689963/1‑94 (MQ=60)

                                                                      GTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTC                         >  SRR4302543.899553/1‑94 (MQ=60)

                                                                      GTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTC                         >  SRR4302543.6820701/1‑94 (MQ=60)

                                                                      GTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTC                         <  SRR4302543.1610326/94‑1 (MQ=60)

                                                                      GTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTC                         >  SRR4302543.1713118/1‑94 (MQ=60)

                                                                        TTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCAT                       <  SRR4302543.81760/94‑1 (MQ=60)

                                                                        TTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGCACTTGCCGTTTTCAT                       <  SRR4302543.4876665/94‑1 (MQ=60)

                                                                         TGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATA                      <  SRR4302543.1425292/94‑1 (MQ=60)

                                                                         TGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATA                      <  SRR4302543.4596264/94‑1 (MQ=60)

                                                                         TGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATA                      >  SRR4302543.4943139/1‑94 (MQ=60)

                                                                         TGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATA                      >  SRR4302543.4943138/1‑94 (MQ=60)

                                                                          GTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATAC                     <  SRR4302543.1980748/94‑1 (MQ=60)

                                                                          GTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATAC                     <  SRR4302543.4198665/94‑1 (MQ=60)

                                                                          GTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGCTATCCACACCTACGTCTTTAATGTCGTACTTGCCGTTTTCATAC                     >  SRR4302543.7160080/1‑94 (MQ=60)

                                                                           TTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACT                    <  SRR4302543.4792680/94‑1 (MQ=60)

                                                                              TTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGA                 <  SRR4302543.4827981/94‑1 (MQ=60)

                                                                               TAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAA                >  SRR4302543.2438198/1‑94 (MQ=60)

                                                                                AATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAAC               <  SRR4302543.951291/94‑1 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTaaccg              >  SRR4302543.5775656/1‑89 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACG              >  SRR4302543.1098969/1‑94 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACG              >  SRR4302543.7243446/1‑94 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACG              <  SRR4302543.17134/94‑1 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACG              >  SRR4302543.1796533/1‑94 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACG              >  SRR4302543.3170546/1‑94 (MQ=60)

                                                                                 ATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTCTCATACTTGAACG              >  SRR4302543.1098968/1‑94 (MQ=60)

                                                                                    AGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCAT           >  SRR4302543.2528067/1‑94 (MQ=60)

                                                                                    AGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCAT           >  SRR4302543.2528068/1‑94 (MQ=60)

                                                                                    AGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCAT           >  SRR4302543.4638300/1‑94 (MQ=60)

                                                                                     GGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATA          <  SRR4302543.2240789/94‑1 (MQ=60)

                                                                                      GTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAC         >  SRR4302543.2545745/1‑94 (MQ=60)

                                                                                      GTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAA         <  SRR4302543.958801/94‑1 (MQ=60)

                                                                                      GTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAA         <  SRR4302543.1327617/94‑1 (MQ=60)

                                                                                       TCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAAC        <  SRR4302543.7431257/94‑1 (MQ=60)

                                                                                       TCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAAC        <  SRR4302543.4962393/94‑1 (MQ=60)

                                                                                       TCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAAC        <  SRR4302543.3298522/94‑1 (MQ=60)

                                                                                        CAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACC       <  SRR4302543.7249415/94‑1 (MQ=60)

                                                                                        CAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACC       >  SRR4302543.5982180/1‑94 (MQ=60)

                                                                                        CAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACC       >  SRR4302543.2255990/1‑94 (MQ=60)

                                                                                        CAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACC       <  SRR4302543.7465391/94‑1 (MQ=60)

                                                                                        CAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACC       <  SRR4302543.4346035/94‑1 (MQ=60)

                                                                                         AACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC      <  SRR4302543.4748819/94‑1 (MQ=60)

                                                                                         AACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC      <  SRR4302543.3636990/94‑1 (MQ=60)

                                                                                         AACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC      <  SRR4302543.6937943/94‑1 (MQ=60)

                                                                                           CCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCC    <  SRR4302543.7676679/94‑1 (MQ=60)

                                                                                           CCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCC    <  SRR4302543.5886766/94‑1 (MQ=60)

                                                                                            CGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCG   >  SRR4302543.7935821/1‑94 (MQ=60)

                                                                                            CGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCG   >  SRR4302543.6440087/1‑94 (MQ=60)

                                                                                            CGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCG   >  SRR4302543.3907490/1‑94 (MQ=60)

                                                                                             GGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  >  SRR4302543.7509033/1‑94 (MQ=60)

                                                                                             GGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  >  SRR4302543.2581981/1‑94 (MQ=60)

                                                                                             GGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  >  SRR4302543.3969695/1‑94 (MQ=60)

                                                                                             GGGAAGGTCAGACCCGCTTTCGCGCCAGCGGTATCCACGCCCACGTCTTTAATGTCCTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  <  SRR4302543.4526864/94‑1 (MQ=60)

ATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGT  >  NC_000913/4243244‑4243430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`