Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,242,957 C→T E348K (GAA→AAA)  malE ← periplasmic maltose‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,9570CT100.0% 953.8 / NA 261E348K (GAA→AAA) malEperiplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (116/145);  total (116/145)

Only 100 of 267 total aligned reads displayed.

TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046
                                                                                          |                                                                                         
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  2:1514111/93‑1 (MQ=255)
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  1:3170105/93‑1 (MQ=255)
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         >  2:1928702/1‑93 (MQ=255)
 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  2:473572/93‑1 (MQ=255)
 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  1:4308469/93‑1 (MQ=255)
 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  2:1028818/93‑1 (MQ=255)
  gcggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtg                                                                                       <  1:3785893/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:840115/93‑1 (MQ=255)
     gcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtg                                                                                    >  2:3690563/1‑93 (MQ=255)
     gcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTCTTTCAtggtg                                                                                    <  2:1205360/93‑1 (MQ=255)
      cgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtgg                                                                                   <  2:2201788/93‑1 (MQ=255)
       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggc                                                                                  >  1:138799/1‑93 (MQ=255)
        ttGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcg                                                                                 >  1:1759706/1‑93 (MQ=255)
          gATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcggc                                                                               <  1:4402992/93‑1 (MQ=255)
           aTCACCGCAGTACGCACGGCATACCGGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCa                                                                              <  1:4466768/93‑1 (MQ=255)
           aTCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCa                                                                              >  1:2688916/1‑93 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  2:6316/93‑1 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  1:2456222/93‑1 (MQ=255)
             cACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAAt                                                                            <  2:3062730/93‑1 (MQ=255)
              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           <  1:3452380/93‑1 (MQ=255)
              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           <  2:4037700/93‑1 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:2314019/93‑1 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:2170684/93‑1 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:3293018/93‑1 (MQ=255)
                    gtgcGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:737341/90‑1 (MQ=255)
                      aCGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATc                                                                   <  1:2405860/93‑1 (MQ=255)
                       cGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCt                                                                  <  2:4337741/93‑1 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 >  2:4385356/1‑93 (MQ=255)
                            ggCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGc                                                             <  1:3729597/93‑1 (MQ=255)
                             gCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                            >  1:4054433/1‑93 (MQ=255)
                              cATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCa                                                           <  1:2500070/93‑1 (MQ=255)
                              cATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCa                                                           >  1:1799209/1‑93 (MQ=255)
                               aTACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCac                                                          >  1:616134/1‑92 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:2111533/1‑93 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATATTTCGCCAAc                                                         >  2:4219578/1‑93 (MQ=255)
                                  cgaGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       <  1:4658085/91‑1 (MQ=255)
                                  ccAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       >  1:4191830/1‑93 (MQ=255)
                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGGGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  2:3864814/1‑93 (MQ=255)
                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  1:903295/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:3546313/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:1960538/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  2:2958742/1‑93 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  1:1896559/93‑1 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGAAATACGTGGATCTTTCGCCAACTCTTcc                                                   >  1:4614538/1‑93 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCGCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:3769809/93‑1 (MQ=255)
                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  1:2768216/93‑1 (MQ=255)
                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaaa                                            >  1:1049329/1‑92 (MQ=255)
                                               aTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGAc                                          <  2:3626057/93‑1 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  1:1474077/1‑93 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:3112779/93‑1 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:204944/93‑1 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       >  1:1305243/1‑93 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       >  2:3932241/1‑93 (MQ=255)
                                                  tGCGGGATGTTCGGAATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:872912/93‑1 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:2430192/93‑1 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:283003/93‑1 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:4592350/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  2:3281195/93‑1 (MQ=255)
                                                     gggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgc                                    <  1:338137/93‑1 (MQ=255)
                                                     gggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgc                                    <  1:2233826/93‑1 (MQ=255)
                                                      ggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcg                                   >  1:3981259/1‑93 (MQ=255)
                                                        aTGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAAGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCt                                 <  1:285374/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               >  2:2635660/1‑93 (MQ=255)
                                                           ttCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACg                              >  2:1959617/1‑93 (MQ=255)
                                                               gCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAc                          >  1:2345648/1‑93 (MQ=255)
                                                                cATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAcc                         <  2:4344477/93‑1 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:2132204/1‑93 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:3615944/1‑93 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:496928/1‑93 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  2:1266232/1‑93 (MQ=255)
                                                                   gATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                      >  1:1682073/1‑93 (MQ=255)
                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  2:7405/1‑93 (MQ=255)
                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  2:3094035/1‑93 (MQ=255)
                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  1:570544/1‑93 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  1:984079/93‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  >  1:2945385/1‑93 (MQ=255)
                                                                        cACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGtt                 <  1:1008911/93‑1 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                >  2:1724850/1‑93 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGgtt                >  2:2445048/1‑93 (MQ=255)
                                                                          ccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTg               <  1:2597040/93‑1 (MQ=255)
                                                                           cTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt              <  1:189582/93‑1 (MQ=255)
                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:2918311/1‑93 (MQ=255)
                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:1606203/1‑93 (MQ=255)
                                                                               ttGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCttt          <  1:3307910/92‑1 (MQ=255)
                                                                               ctaggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCttt          >  2:1864980/4‑93 (MQ=255)
                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         <  1:452974/93‑1 (MQ=255)
                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         >  1:1165013/1‑93 (MQ=255)
                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         <  2:2748244/93‑1 (MQ=255)
                                                                                 tggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        <  1:2097359/92‑1 (MQ=255)
                                                                                 gggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        >  1:2899696/1‑93 (MQ=255)
                                                                                  tgCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:1170918/2‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:1371419/1‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  2:4493072/93‑1 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  2:3421404/93‑1 (MQ=255)
                                                                                   gCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatta      >  2:4109639/1‑93 (MQ=255)
                                                                                   gCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatta      >  2:109999/1‑93 (MQ=255)
                                                                                     gTTTTTCATGGTGGCGGCATTACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:4551804/1‑93 (MQ=255)
                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:2828968/1‑93 (MQ=255)
                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  1:3927886/1‑93 (MQ=255)
                                                                                      ttttTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc   <  2:674438/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  >  NC_000913/4242864‑4243050
                                                                                             |                                                                                             
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTT                                                                                               <  SRR4302535.132366/94‑1 (MQ=60)
 GCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTC                                                                                              >  SRR4302535.4318529/1‑94 (MQ=60)
  CTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCA                                                                                             <  SRR4302535.1726771/94‑1 (MQ=60)
   TGGCGGCGTTGATCACCGCAGTACGCACGGCATACGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAT                                                                                            <  SRR4302535.4611505/94‑1 (MQ=60)
   TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAT                                                                                            <  SRR4302535.3192059/94‑1 (MQ=60)
    GGGGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGAATTCACCTTTCTGGGCGTTTTTCATG                                                                                           >  SRR4302535.4560676/1‑94 (MQ=60)
    GGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATG                                                                                           <  SRR4302535.4337945/94‑1 (MQ=60)
    GGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATG                                                                                           <  SRR4302535.2713661/94‑1 (MQ=60)
     GCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGG                                                                                          <  SRR4302535.3812008/94‑1 (MQ=60)
     GCGGCGTTGATCACCGAGGTGCGAACGGCATACGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGG                                                                                          <  SRR4302535.755731/94‑1 (MQ=60)
      CGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGT                                                                                         <  SRR4302535.3564446/94‑1 (MQ=60)
       tcttttttttttcaagcagaagACGGCATACGAGATAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTG                                                                                        <  SRR4302535.4380520/72‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCCCGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302535.1035018/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302535.3809479/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302535.601456/94‑1 (MQ=60)
       GGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTG                                                                                        <  SRR4302535.846014/94‑1 (MQ=60)
         CGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGC                                                                                      >  SRR4302535.139771/1‑94 (MQ=60)
         CGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGC                                                                                      <  SRR4302535.4065810/94‑1 (MQ=60)
         CGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGC                                                                                      >  SRR4302535.4221772/1‑94 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGTCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGAG                                                                                     <  SRR4302535.2617001/94‑1 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     <  SRR4302535.202680/94‑1 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     >  SRR4302535.2160227/1‑94 (MQ=60)
          GTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCG                                                                                     >  SRR4302535.1772446/1‑94 (MQ=60)
           ttgtttttcaagcagaagACGGCATACGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGG                                                                                    <  SRR4302535.3960198/76‑1 (MQ=60)
            TGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGC                                                                                   <  SRR4302535.2254740/94‑1 (MQ=60)
             GATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCA                                                                                  >  SRR4302535.2708133/1‑94 (MQ=60)
             GATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCA                                                                                  <  SRR4302535.4433123/94‑1 (MQ=60)
              ATCACCGCAGTACGCACGGCATACCGGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAA                                                                                 <  SRR4302535.4497414/94‑1 (MQ=60)
              ATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAA                                                                                 >  SRR4302535.4711574/1‑94 (MQ=60)
               TCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAAT                                                                                <  SRR4302535.2473932/94‑1 (MQ=60)
                 TCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAC                                                                              <  SRR4302535.4434211/94‑1 (MQ=60)
                 ACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAC                                                                              <  SRR4302535.3476376/94‑1 (MQ=60)
                   CGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGT                                                                            >  SRR4302535.1735014/1‑94 (MQ=60)
                   CGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGG                                                                            >  SRR4302535.3616580/1‑94 (MQ=60)
                     CAGTACGCACGGCATACCAGAAATCTGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          <  SRR4302535.1807394/94‑1 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          >  SRR4302535.1300604/1‑94 (MQ=60)
                     CAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGG                                                                          <  SRR4302535.3123160/94‑1 (MQ=60)
                      AGTACGCACGGCATACCAGAAAGCGGACATCTGCGTGATTTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGA                                                                         <  SRR4302535.939382/94‑1 (MQ=60)
                      AGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGA                                                                         >  SRR4302535.3388775/1‑94 (MQ=60)
                       GTGCGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302535.742526/94‑1 (MQ=60)
                       GTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302535.2287950/94‑1 (MQ=60)
                       GCACGCACGGCATACCAGAAAGCGGACATCTGCGGCATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAT                                                                        <  SRR4302535.671384/94‑1 (MQ=60)
                        ggaagACGGCATACGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATC                                                                       <  SRR4302535.2994891/89‑1 (MQ=60)
                         GCGCACGGCATACGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCT                                                                      <  SRR4302535.3878169/94‑1 (MQ=60)
                         ACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCT                                                                      <  SRR4302535.2423176/94‑1 (MQ=60)
                           GCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTT                                                                    >  SRR4302535.4682589/1‑94 (MQ=60)
                            CACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTC                                                                   <  SRR4302535.4618700/94‑1 (MQ=60)
                             ACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCG                                                                  <  SRR4302535.1448993/94‑1 (MQ=60)
                               GGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCC                                                                <  SRR4302535.3755425/94‑1 (MQ=60)
                               GGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCC                                                                >  SRR4302535.4082195/1‑94 (MQ=60)
                                GCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCA                                                               >  SRR4302535.1812218/1‑94 (MQ=60)
                                 CATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAC                                                              >  SRR4302535.620418/1‑94 (MQ=60)
                                 CATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAA                                                              <  SRR4302535.2518103/94‑1 (MQ=60)
                                  ctacgagatAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAC                                                             <  SRR4302535.2879008/85‑1 (MQ=60)
                                    ACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTC                                                           >  SRR4302535.4220516/1‑94 (MQ=60)
                                     CGAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCT                                                          <  SRR4302535.4689598/94‑1 (MQ=60)
                                     CCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCT                                                          <  SRR4302535.2201628/94‑1 (MQ=60)
                                      CAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTT                                                         >  SRR4302535.909694/1‑94 (MQ=60)
                                      CAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTT                                                         <  SRR4302535.1630538/94‑1 (MQ=60)
                                      CAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTT                                                         >  SRR4302535.2393743/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302535.3570935/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302535.1165176/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302535.2572301/1‑94 (MQ=60)
                                       AGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTC                                                        >  SRR4302535.1974680/1‑94 (MQ=60)
                                        GAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGAAATACGTGGATCTTTCGCCAACTCTTCC                                                       >  SRR4302535.4645892/1‑94 (MQ=60)
                                         AAAGCGGATATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCT                                                      <  SRR4302535.3801727/94‑1 (MQ=60)
                                         AAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCT                                                      <  SRR4302535.1910205/94‑1 (MQ=60)
                                         AAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCT                                                      <  SRR4302535.1160833/94‑1 (MQ=60)
                                         AAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCT                                                      >  SRR4302535.1875516/1‑94 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCGCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302535.3795826/94‑1 (MQ=60)
                                          AAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTC                                                     <  SRR4302535.4424052/94‑1 (MQ=60)
                                           AGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCG                                                    <  SRR4302535.1482810/94‑1 (MQ=60)
                                           AGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCG                                                    <  SRR4302535.4638558/94‑1 (MQ=60)
                                               gagagCTGCGGGATGGTCGGCAGTATTTCACCTTTCTGGTCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302535.932575/89‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302535.54314/94‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302535.1004837/94‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                >  SRR4302535.1922796/1‑94 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAG                                                <  SRR4302535.2787926/94‑1 (MQ=60)
                                               GACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAA                                                >  SRR4302535.1056794/1‑94 (MQ=60)
                                                ACNTNTNCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGA                                               <  SRR4302535.1575009/94‑1 (MQ=60)
                                                  ATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACT                                             >  SRR4302535.1484630/1‑94 (MQ=60)
                                                   TCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTT                                            <  SRR4302535.2494140/94‑1 (MQ=60)
                                                    CTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTC                                           >  SRR4302535.1314561/1‑94 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          >  SRR4302535.267449/1‑94 (MQ=60)
                                                     TGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCA                                          <  SRR4302535.1510334/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302535.284977/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302535.2447701/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302535.435431/94‑1 (MQ=60)
                                                      GCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAG                                         <  SRR4302535.4623609/94‑1 (MQ=60)
                                                       CGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCTGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGC                                        <  SRR4302535.190910/94‑1 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       >  SRR4302535.2795235/1‑94 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       <  SRR4302535.340476/94‑1 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       >  SRR4302535.3405237/1‑94 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       >  SRR4302535.4008456/1‑94 (MQ=60)
                                                        GGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCG                                       <  SRR4302535.2249713/94‑1 (MQ=60)
                                                          GATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCT                                     <  SRR4302535.3651529/94‑1 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTC                                    >  SRR4302535.2947827/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    >  SRR4302535.2453283/1‑94 (MQ=60)
                                                           ATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAAGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTA                                    <  SRR4302535.287370/94‑1 (MQ=60)
                                                             GTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACG                                  <  SRR4302535.3998462/94‑1 (MQ=60)
                                                                 GGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAC                              >  SRR4302535.2362459/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302535.3641011/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302535.500414/1‑94 (MQ=60)
                                                                   CATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCC                            >  SRR4302535.2147575/1‑94 (MQ=60)
                                                                    ATGATTGCACCTGTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCA                           <  SRR4302535.2437997/94‑1 (MQ=60)
                                                                     TGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAG                          >  SRR4302535.1694257/1‑94 (MQ=60)
                                                                      GATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGC                         >  SRR4302535.574551/1‑94 (MQ=60)
                                                                      GATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCAAGC                         >  SRR4302535.3885940/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        >  SRR4302535.794460/1‑94 (MQ=60)
                                                                       ATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCG                        <  SRR4302535.4171561/94‑1 (MQ=60)
                                                                        TTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGG                       >  SRR4302535.89548/1‑94 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      <  SRR4302535.1359802/94‑1 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      <  SRR4302535.3351413/94‑1 (MQ=60)
                                                                         TTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGT                      >  SRR4302535.2966086/1‑94 (MQ=60)
                                                                          TCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTT                     <  SRR4302535.991068/94‑1 (MQ=60)
                                                                           CACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTT                    <  SRR4302535.1016080/94‑1 (MQ=60)
                                                                            CCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTG                   <  SRR4302535.1272331/94‑1 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGT                  <  SRR4302535.2615691/94‑1 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGT                  >  SRR4302535.3594683/1‑94 (MQ=60)
                                                                             CCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGCTTTGT                  >  SRR4302535.2855232/1‑94 (MQ=60)
                                                                              CTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTC                 >  SRR4302535.1808193/1‑94 (MQ=60)
                                                                              CTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTC                 >  SRR4302535.3763960/1‑94 (MQ=60)
                                                                              CTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTC                 <  SRR4302535.190890/94‑1 (MQ=60)
                                                                              CTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGAGCTACGGCACCCAGCGGTTTGTC                 >  SRR4302535.4709721/1‑94 (MQ=60)
                                                                               TTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCT                >  SRR4302535.4435452/1‑94 (MQ=60)
                                                                                TTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTT               <  SRR4302535.954084/94‑1 (MQ=60)
                                                                                TTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTT               >  SRR4302535.4628362/1‑94 (MQ=60)
                                                                                 TCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTT              <  SRR4302535.1887986/94‑1 (MQ=60)
                                                                                  TTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             <  SRR4302535.3330929/94‑1 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302535.1173335/1‑94 (MQ=60)
                                                                                  CTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTA             >  SRR4302535.1952175/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302535.28691/94‑1 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302535.2449414/94‑1 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            >  SRR4302535.2108833/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            >  SRR4302535.2920198/1‑94 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302535.3662264/94‑1 (MQ=60)
                                                                                   TGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTAT            <  SRR4302535.456148/94‑1 (MQ=60)
                                                                                    TGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATT           <  SRR4302535.2112486/94‑1 (MQ=60)
                                                                                    GGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATT           <  SRR4302535.4163345/94‑1 (MQ=60)
                                                                                    GGGCCTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATT           <  SRR4302535.213660/94‑1 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          >  SRR4302535.4052773/1‑94 (MQ=60)
                                                                                     GGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTA          <  SRR4302535.729145/94‑1 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        >  SRR4302535.2940175/1‑94 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        >  SRR4302535.3170141/1‑94 (MQ=60)
                                                                                       CGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAC        >  SRR4302535.3954717/1‑94 (MQ=60)
                                                                                        GTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACC       >  SRR4302535.2604753/1‑94 (MQ=60)
                                                                                         TTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG      >  SRR4302535.2443535/1‑94 (MQ=60)
                                                                                         TTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG      <  SRR4302535.2792273/94‑1 (MQ=60)
                                                                                         TTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG      <  SRR4302535.3285416/94‑1 (MQ=60)
                                                                                         TTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG      >  SRR4302535.3443918/1‑94 (MQ=60)
                                                                                          TTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGC     <  SRR4302535.508064/94‑1 (MQ=60)
                                                                                          TTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGC     >  SRR4302535.3603635/1‑94 (MQ=60)
                                                                                           TTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCT    >  SRR4302535.3887544/1‑94 (MQ=60)
                                                                                           TTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCT    >  SRR4302535.3580886/1‑94 (MQ=60)
                                                                                            TTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTT   <  SRR4302535.177209/94‑1 (MQ=60)
                                                                                             TCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  <  SRR4302535.958009/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTC  >  NC_000913/4242864‑4243050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑