Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,251,782	A→G	V755A (GTG→GCG)	plsB ←	glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,251,782	0	A	G	99.2%	785.8 / NA	258	V755A (GTG→GCG)	plsB	glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (141/114); total (144/114)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACC  >  NC_000913/4251692‑4251870

 gATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCg                                                                                       >  1:4210739/1‑93 (MQ=255)

  aTAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAAcgccgc                                                                                      >  2:1269142/1‑93 (MQ=255)

    aaaCCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCt                                                                                    <  2:2184125/93‑1 (MQ=255)

    aaaCCTTCATCGTTTCTGCCGGTTCGGCAGCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCt                                                                                    <  2:4499803/93‑1 (MQ=255)

      aCCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTg                                                                                  >  2:3109901/1‑93 (MQ=255)

      aCCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTg                                                                                  >  1:2438173/1‑93 (MQ=255)

        cTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTc                                                                                >  1:3745849/1‑93 (MQ=255)

         ttCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCg                                                                               >  1:2962965/1‑93 (MQ=255)

           cATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaa                                                                             <  1:2895918/93‑1 (MQ=255)

            aTCGTTTCAGCCGGTTCGGCCTCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaag                                                                            >  2:4583064/1‑93 (MQ=255)

             tCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGACGCCTTGTCgaaga                                                                           >  2:1951744/1‑93 (MQ=255)

               gTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAc                                                                         >  1:3843610/1‑93 (MQ=255)

                tttCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACt                                                                        >  2:220868/1‑93 (MQ=255)

                  tCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTcc                                                                      <  2:3626347/93‑1 (MQ=255)

                  tCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTcc                                                                      <  1:1465220/93‑1 (MQ=255)

                   cTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCg                                                                     <  2:4183835/93‑1 (MQ=255)

                    tGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCgg                                                                    >  1:626819/1‑93 (MQ=255)

                     gCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGgc                                                                   <  1:3910733/93‑1 (MQ=255)

                     gCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGgc                                                                   >  1:357898/1‑93 (MQ=255)

                     gCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTCAACGCCGCCTTGTCGAAGAACTCCGgc                                                                   >  2:564312/1‑93 (MQ=255)

                       cGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTACGgcgc                                                                 >  2:2641774/1‑93 (MQ=255)

                        ggTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGcgcg                                                                >  1:343014/1‑93 (MQ=255)

                        cgTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGcgcg                                                                <  1:1061581/92‑1 (MQ=255)

                         gTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGt                                                               >  2:2506620/1‑93 (MQ=255)

                          ttCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAAGTCAGGCGCGtt                                                              >  2:2573880/1‑93 (MQ=255)

                           tCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTg                                                             >  2:3821229/1‑93 (MQ=255)

                            cGGCATCGCCGCTATCGCTGATATGCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                            <  1:3241299/93‑1 (MQ=255)

                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                            <  1:3098317/93‑1 (MQ=255)

                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                            <  2:1676833/93‑1 (MQ=255)

                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                            <  2:2267518/93‑1 (MQ=255)

                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                            <  1:1080089/93‑1 (MQ=255)

                             ggCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAt                                                           <  2:2723629/93‑1 (MQ=255)

                              gCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATg                                                          >  1:427977/1‑93 (MQ=255)

                                aTCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGcc                                                        >  1:4547039/1‑93 (MQ=255)

                                 tCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCg                                                       <  1:2633941/93‑1 (MQ=255)

                                  cgccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGt                                                      <  1:1334322/93‑1 (MQ=255)

                                  cgccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGt                                                      >  1:1189123/1‑93 (MQ=255)

                                     cgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGGCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgca                                                   >  1:4340760/1‑93 (MQ=255)

                                     cgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTACGGCGCGCTGATGCCATgca                                                   >  2:4278976/1‑93 (MQ=255)

                                      gcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcag                                                  <  1:365555/93‑1 (MQ=255)

                                        tATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagca                                                >  1:3607402/1‑93 (MQ=255)

                                              tGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga                                          >  2:3831416/1‑93 (MQ=255)

                                              tGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga                                          >  2:3124427/1‑93 (MQ=255)

                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGACCTGAACGCCGCCTTGTCGAAGAGCTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                         <  1:2235310/93‑1 (MQ=255)

                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                         <  2:2395743/93‑1 (MQ=255)

                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                         >  1:57742/1‑93 (MQ=255)

                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                         >  1:706303/1‑93 (MQ=255)

                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAATCCGGGGCGTTGATGCCGCGCAGCACGgagag                                         >  2:625359/1‑93 (MQ=255)

                                                 tataCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAc                                       <  2:4646310/93‑1 (MQ=255)

                                                   taCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGCGACGt                                     >  2:1903164/1‑93 (MQ=255)

                                                   taCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTCGTGCCGTGCAGAACGGAGAGACGt                                     >  2:855572/1‑93 (MQ=255)

                                                   taCCCTTCATCACGCAGCGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGt                                     >  2:750935/1‑93 (MQ=255)

                                                    aCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGtt                                    <  2:4311823/93‑1 (MQ=255)

                                                     cccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTg                                   >  1:3183910/1‑93 (MQ=255)

                                                     cccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTg                                   >  1:2762895/1‑93 (MQ=255)

                                                     cccTTCATCACCCAGTGTCAGCACCAGAGAACTGACCCCCGCCTTGTCGAAGCACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTg                                   <  2:4091613/93‑1 (MQ=255)

                                                      ccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCCAGGAGAGACGTTgc                                  >  2:1241345/1‑93 (MQ=255)

                                                      ccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgc                                  <  2:1413809/93‑1 (MQ=255)

                                                      ccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgc                                  >  2:1816241/1‑93 (MQ=255)

                                                      ccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgc                                  >  1:1809150/1‑93 (MQ=255)

                                                       cTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgcg                                 >  2:2891510/1‑93 (MQ=255)

                                                       cTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgcg                                 >  1:1600851/1‑93 (MQ=255)

                                                       cTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgcg                                 >  2:2052210/1‑93 (MQ=255)

                                                        ttCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgcgc                                >  2:2819594/1‑93 (MQ=255)

                                                           atcaCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGAc                             >  1:3788019/1‑93 (MQ=255)

                                                              aCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGGCGGTGCGcggcggg                          >  2:3200649/1‑92 (MQ=255)

                                                              aCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGt                          >  1:1349009/1‑93 (MQ=255)

                                                              aCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGt                          >  2:806068/1‑93 (MQ=255)

                                                                gCAGTGTCAGCACCAGAGAACTGGACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGc                        <  1:971801/93‑1 (MQ=255)

                                                                gCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGc                        >  2:1366163/1‑93 (MQ=255)

                                                                   gtgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGc                     <  1:908523/93‑1 (MQ=255)

                                                                   gtgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGc                     <  1:4077196/93‑1 (MQ=255)

                                                                       cAGCCCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctc                 <  2:1487447/93‑1 (MQ=255)

                                                                       cAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctc                 >  1:1257420/1‑93 (MQ=255)

                                                                       cAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctc                 <  1:1578263/93‑1 (MQ=255)

                                                                       cAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctc                 <  1:1935409/93‑1 (MQ=255)

                                                                       cAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctc                 <  1:4048739/93‑1 (MQ=255)

                                                                        aGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctct                >  1:488163/1‑93 (MQ=255)

                                                                         gCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCtt               >  2:4669870/1‑93 (MQ=255)

                                                                           aCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc             <  1:928271/93‑1 (MQ=255)

                                                                           aCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc             >  1:4675890/1‑93 (MQ=255)

                                                                           aCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc             >  2:3486828/1‑93 (MQ=255)

                                                                           aCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc             <  1:2076616/93‑1 (MQ=255)

                                                                            ccAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtct            >  1:826970/1‑93 (MQ=255)

                                                                             cAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtctc           >  1:1692390/1‑93 (MQ=255)

                                                                              agagAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTcc          >  1:2484685/1‑93 (MQ=255)

                                                                              agagAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTcc          >  2:1585496/1‑93 (MQ=255)

                                                                              agagAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTcc          >  1:1096875/1‑93 (MQ=255)

                                                                              agagAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTcc          >  1:3285234/1‑93 (MQ=255)

                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa         >  2:2849637/1‑93 (MQ=255)

                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa         >  1:1404239/1‑93 (MQ=255)

                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa         <  1:1121243/93‑1 (MQ=255)

                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa         >  2:2342027/1‑93 (MQ=255)

                                                                                agaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAg        >  1:4475332/1‑93 (MQ=255)

                                                                                 gaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGc       >  2:2794981/1‑93 (MQ=255)

                                                                                   aCTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCga     >  2:933334/1‑92 (MQ=255)

                                                                                     tGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAc   <  2:3048905/93‑1 (MQ=255)

                                                                                      gAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAcg  >  1:2154158/1‑92 (MQ=255)

                                                                                      gAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAcc  <  1:250265/93‑1 (MQ=255)

                                                                                      gAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAcc  <  2:2927947/93‑1 (MQ=255)

TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACC  >  NC_000913/4251692‑4251870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  >  NC_000913/4251689‑4251875

AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACG                                                                                               <  SRR4302535.370316/94‑1 (MQ=60)

  CTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCC                                                                                             >  SRR4302535.3459231/1‑94 (MQ=60)

  CTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCC                                                                                             >  SRR4302535.1935581/1‑94 (MQ=60)

   TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCG                                                                                            >  SRR4302535.2371834/1‑94 (MQ=60)

   TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCG                                                                                            >  SRR4302535.4239558/1‑94 (MQ=60)

    GATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGC                                                                                           >  SRR4302535.2613255/1‑94 (MQ=60)

       AAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTT                                                                                        <  SRR4302535.931257/94‑1 (MQ=60)

        AACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTG                                                                                       >  SRR4302535.2455737/1‑94 (MQ=60)

         ACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGT                                                                                      >  SRR4302535.4324161/1‑94 (MQ=60)

         ACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCATGT                                                                                      <  SRR4302535.108913/94‑1 (MQ=60)

          CCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTC                                                                                     >  SRR4302535.3771767/1‑94 (MQ=60)

          CCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTC                                                                                     >  SRR4302535.2949394/1‑94 (MQ=60)

          CCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTC                                                                                     <  SRR4302535.89487/94‑1 (MQ=60)

           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGGACTGAACTCCGCCTTGTCG                                                                                    >  SRR4302535.473622/1‑94 (MQ=60)

           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    >  SRR4302535.4128549/1‑94 (MQ=60)

           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    <  SRR4302535.2817395/94‑1 (MQ=60)

           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    <  SRR4302535.1501698/94‑1 (MQ=60)

           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    >  SRR4302535.2983764/1‑94 (MQ=60)

            TTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGA                                                                                   >  SRR4302535.108247/1‑94 (MQ=60)

            TTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGA                                                                                   >  SRR4302535.3657277/1‑94 (MQ=60)

              CATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAG                                                                                 <  SRR4302535.2916398/94‑1 (MQ=60)

                 CGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAC                                                                              <  SRR4302535.4693438/94‑1 (MQ=60)

                 CGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAC                                                                              >  SRR4302535.3870085/1‑94 (MQ=60)

                 CGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAC                                                                              >  SRR4302535.248791/1‑94 (MQ=60)

                  GTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACT                                                                             <  SRR4302535.4078275/94‑1 (MQ=60)

                  GTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACT                                                                             >  SRR4302535.2656178/1‑94 (MQ=60)

                    TTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCC                                                                           >  SRR4302535.552224/1‑94 (MQ=60)

                     TCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCG                                                                          <  SRR4302535.1475717/94‑1 (MQ=60)

                     TCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCG                                                                          <  SRR4302535.1546432/94‑1 (MQ=60)

                      CTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGG                                                                         >  SRR4302535.631182/1‑94 (MQ=60)

                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        >  SRR4302535.818006/1‑94 (MQ=60)

                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        >  SRR4302535.360373/1‑94 (MQ=60)

                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        <  SRR4302535.1215291/94‑1 (MQ=60)

                        GCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCG                                                                       <  SRR4302535.727228/94‑1 (MQ=60)

                        GCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCG                                                                       <  SRR4302535.3937509/94‑1 (MQ=60)

                        GCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCC                                                                       >  SRR4302535.4072762/1‑94 (MQ=60)

                          CGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCG                                                                     <  SRR4302535.1766929/94‑1 (MQ=60)

                          CGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCG                                                                     >  SRR4302535.345386/1‑94 (MQ=60)

                          CGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCG                                                                     >  SRR4302535.2188891/1‑94 (MQ=60)

                           GGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGT                                                                    <  SRR4302535.2851419/94‑1 (MQ=60)

                           CGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGT                                                                    <  SRR4302535.1069141/94‑1 (MQ=60)

                             TTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTG                                                                  >  SRR4302535.1229962/1‑94 (MQ=60)

                              TCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGA                                                                 >  SRR4302535.511353/1‑94 (MQ=60)

                               CGGCATCGCCGCTATCGCTGATATGCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302535.3263808/94‑1 (MQ=60)

                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302535.3119889/94‑1 (MQ=60)

                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302535.1087773/94‑1 (MQ=60)

                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302535.1117302/94‑1 (MQ=60)

                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               >  SRR4302535.430954/1‑94 (MQ=60)

                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               >  SRR4302535.946898/1‑94 (MQ=60)

                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               <  SRR4302535.1324104/94‑1 (MQ=60)

                                 GCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGC                                                              <  SRR4302535.2221282/94‑1 (MQ=60)

                                 GCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGC                                                              <  SRR4302535.1775803/94‑1 (MQ=60)

                                  CATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCC                                                             >  SRR4302535.4578129/1‑94 (MQ=60)

                                  CATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCC                                                             >  SRR4302535.4079069/1‑94 (MQ=60)

                                   ATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCG                                                            >  SRR4302535.1377370/1‑94 (MQ=60)

                                    TCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGT                                                           <  SRR4302535.2652818/94‑1 (MQ=60)

                                    TCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGT                                                           >  SRR4302535.1197640/1‑94 (MQ=60)

                                     CGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTG                                                          <  SRR4302535.1343839/94‑1 (MQ=60)

                                     CGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTG                                                          >  SRR4302535.1281931/1‑94 (MQ=60)

                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         <  SRR4302535.3664910/94‑1 (MQ=60)

                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         <  SRR4302535.4493502/94‑1 (MQ=60)

                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         >  SRR4302535.2622276/1‑94 (MQ=60)

                                       CCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACTAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCA                                                        >  SRR4302535.3102401/1‑94 (MQ=60)

                                       CCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGGCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCA                                                        >  SRR4302535.4370448/1‑94 (MQ=60)

                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       <  SRR4302535.4275615/94‑1 (MQ=60)

                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       >  SRR4302535.371163/1‑94 (MQ=60)

                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       <  SRR4302535.2456216/94‑1 (MQ=60)

                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       >  SRR4302535.1589993/1‑94 (MQ=60)

                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       >  SRR4302535.380830/1‑94 (MQ=60)

                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      <  SRR4302535.368069/94‑1 (MQ=60)

                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      >  SRR4302535.1159583/1‑94 (MQ=60)

                                          CTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCA                                                     >  SRR4302535.3632422/1‑94 (MQ=60)

                                           TATCGCTGATATCCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    <  SRR4302535.67043/94‑1 (MQ=60)

                                               GCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAG                                                >  SRR4302535.489731/1‑94 (MQ=60)

                                               GCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAG                                                >  SRR4302535.3435840/1‑94 (MQ=60)

                                                CTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGA                                               >  SRR4302535.65159/1‑94 (MQ=60)

                                                CTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGA                                               <  SRR4302535.3867630/94‑1 (MQ=60)

                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              >  SRR4302535.58164/1‑94 (MQ=60)

                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              >  SRR4302535.711264/1‑94 (MQ=60)

                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              >  SRR4302535.1970391/1‑94 (MQ=60)

                                                  GATATACCCTTCATCACGCAGTGTCAGCACCAGAGACCTGAACGCCGCCTTGTCGAAGAGCTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGA                                             <  SRR4302535.2251212/94‑1 (MQ=60)

                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            >  SRR4302535.2776684/1‑94 (MQ=60)

                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            >  SRR4302535.2514525/1‑94 (MQ=60)

                                                     ATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGT                                          >  SRR4302535.4668819/1‑94 (MQ=60)

                                                     ATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGT                                          >  SRR4302535.2353607/1‑94 (MQ=60)

                                                      TACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTG                                         >  SRR4302535.3811413/1‑94 (MQ=60)

                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        >  SRR4302535.3205903/1‑94 (MQ=60)

                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        >  SRR4302535.2782581/1‑94 (MQ=60)

                                                        CCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGC                                       >  SRR4302535.1822241/1‑94 (MQ=60)

                                                         CCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCG                                      >  SRR4302535.3900060/1‑94 (MQ=60)

                                                         CCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCG                                      <  SRR4302535.4013616/94‑1 (MQ=60)

                                                         CCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCG                                      >  SRR4302535.1612399/1‑94 (MQ=60)

                                                         CCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCG                                      <  SRR4302535.3381937/94‑1 (MQ=60)

                                                           TTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAATTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGATGCGCG                                    >  SRR4302535.3051934/1‑94 (MQ=60)

                                                           TTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCG                                    <  SRR4302535.895706/94‑1 (MQ=60)

                                                             CATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGAC                                  >  SRR4302535.3814146/1‑94 (MQ=60)

                                                              ATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACG                                 >  SRR4302535.3768564/1‑94 (MQ=60)

                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               >  SRR4302535.1358643/1‑94 (MQ=60)

                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               >  SRR4302535.3294588/1‑94 (MQ=60)

                                                                 ACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTG                              >  SRR4302535.4355173/1‑94 (MQ=60)

                                                                 ACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTG                              <  SRR4302535.1877840/94‑1 (MQ=60)

                                                                 ACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTG                              <  SRR4302535.1606211/94‑1 (MQ=60)

                                                                  CGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGG                             >  SRR4302535.1428932/1‑94 (MQ=60)

                                                                  CGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGC                             <  SRR4302535.35749/94‑1 (MQ=60)

                                                                  CGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGC                             <  SRR4302535.3934437/94‑1 (MQ=60)

                                                                  CGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGC                             >  SRR4302535.1459582/1‑94 (MQ=60)

                                                                   GCAGTGTCAGCACCAGAGAACTGGACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCG                            <  SRR4302535.978696/94‑1 (MQ=60)

                                                                     AGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGC                          >  SRR4302535.320322/1‑94 (MQ=60)

                                                                     AGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGC                          >  SRR4302535.1350951/1‑94 (MQ=60)

                                                                     AGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGC                          <  SRR4302535.4455773/94‑1 (MQ=60)

                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         <  SRR4302535.2007075/94‑1 (MQ=60)

                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         <  SRR4302535.914973/94‑1 (MQ=60)

                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         <  SRR4302535.4105094/94‑1 (MQ=60)

                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         >  SRR4302535.16724/1‑94 (MQ=60)

                                                                        GTCAGCACCAGAGAGCTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCT                       <  SRR4302535.4250359/94‑1 (MQ=60)

                                                                         TCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTC                      >  SRR4302535.1266415/1‑94 (MQ=60)

                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     >  SRR4302535.491586/1‑94 (MQ=60)

                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     <  SRR4302535.324552/94‑1 (MQ=60)

                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     <  SRR4302535.4076454/94‑1 (MQ=60)

                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     <  SRR4302535.1589654/94‑1 (MQ=60)

                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     <  SRR4302535.1949349/94‑1 (MQ=60)

                                                                           ggcgCCAGAGAAGTGAACGCCGTCTTGGCGAAGAACTCCGGCGCGTAGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    <  SRR4302535.412180/90‑1 (MQ=60)

                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    <  SRR4302535.974159/94‑1 (MQ=60)

                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    <  SRR4302535.1355872/94‑1 (MQ=60)

                                                                             CACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTC                  <  SRR4302535.1115807/94‑1 (MQ=60)

                                                                             CACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTC                  >  SRR4302535.4707453/1‑94 (MQ=60)

                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 <  SRR4302535.934843/94‑1 (MQ=60)

                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 >  SRR4302535.832759/1‑94 (MQ=60)

                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 <  SRR4302535.2091576/94‑1 (MQ=60)

                                                                               CCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTC                >  SRR4302535.1704631/1‑94 (MQ=60)

                                                                                CAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCC               >  SRR4302535.1104685/1‑94 (MQ=60)

                                                                                CAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCC               >  SRR4302535.2502597/1‑94 (MQ=60)

                                                                                CAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCC               >  SRR4302535.3308069/1‑94 (MQ=60)

                                                                                 AGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCA              >  SRR4302535.1414256/1‑94 (MQ=60)

                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             <  SRR4302535.1129238/94‑1 (MQ=60)

                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             >  SRR4302535.4506039/1‑94 (MQ=60)

                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGC            <  SRR4302535.3269783/94‑1 (MQ=60)

                                                                                     AACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGT          >  SRR4302535.596407/1‑94 (MQ=60)

                                                                                      ACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTA         >  SRR4302535.2159086/1‑94 (MQ=60)

                                                                                      ACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTA         >  SRR4302535.1100436/1‑94 (MQ=60)

                                                                                       CTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAC        <  SRR4302535.3122010/94‑1 (MQ=60)

                                                                                        TGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACG       >  SRR4302535.2169639/1‑94 (MQ=60)

                                                                                        TGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACC       >  SRR4302535.4531261/1‑94 (MQ=60)

                                                                                         GAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG      <  SRR4302535.251990/94‑1 (MQ=60)

                                                                                         GAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG      <  SRR4302535.3626186/94‑1 (MQ=60)

                                                                                           ACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCG    <  SRR4302535.842312/94‑1 (MQ=60)

                                                                                           ACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCG    <  SRR4302535.2314148/94‑1 (MQ=60)

                                                                                            CGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGG   >  SRR4302535.4405137/1‑94 (MQ=60)

                                                                                            CGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGG   >  SRR4302535.1037640/1‑94 (MQ=60)

                                                                                             GCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  <  SRR4302535.1066487/94‑1 (MQ=60)

                                                                                             CCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  <  SRR4302535.810432/94‑1 (MQ=60)

AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  >  NC_000913/4251689‑4251875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`