Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,259,162 A→G intergenic (+277/‑86) yjbM → / → yjbN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,259,1620AG99.7% 1203.3 / ‑5.5 374intergenic (+277/‑86)yjbM/yjbNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (187/186);  total (188/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 406 total aligned reads displayed.

TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251
                                                                                          |                                                                                         
ttattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTatggtatggt                                                                                         <  2:344829/93‑1 (MQ=255)
ttattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTatggtatggt                                                                                         >  2:7443303/1‑93 (MQ=255)
 tattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCATTGAGTCAGGCGCTTCCTGTTGTTAtggtatggta                                                                                        >  2:3143767/1‑93 (MQ=255)
 tattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTAtggtatggta                                                                                        >  1:1609606/1‑93 (MQ=255)
  attTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTAc                                                                                       >  1:7463046/1‑93 (MQ=255)
   tttCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAcc                                                                                      >  2:6940479/1‑93 (MQ=255)
         tCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGTGCTTCCTGTTGTTATGGTATGGTACCCCGCTa                                                                                <  1:6473966/93‑1 (MQ=255)
         tCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTa                                                                                <  2:4465579/93‑1 (MQ=255)
         tCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTa                                                                                >  2:1982724/1‑93 (MQ=255)
          cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAt                                                                               <  1:5899158/93‑1 (MQ=255)
          cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAt                                                                               <  1:4184109/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              <  2:3630610/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  1:7143617/1‑93 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  2:3903795/1‑93 (MQ=255)
            cTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTg                                                                             <  2:1516148/93‑1 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            >  1:4948629/1‑93 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            <  1:3554937/93‑1 (MQ=255)
             ttCGGGAGAGCTTCATGCCTTGAGAGGAACGCAAATTTCTCATGCAATGAGACAGGCGCTGCCCGGTGTTAGGGTATGGTACCCCGCGACTGa                                                                            >  1:7009027/1‑93 (MQ=255)
                gTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGTTATGGTACCCCGCTATTGAGcc                                                                         >  1:5058046/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  1:4868872/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  2:872514/1‑93 (MQ=255)
                  gagaGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctc                                                                       >  1:6093165/1‑93 (MQ=255)
                  gagaGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctc                                                                       >  2:7559526/1‑93 (MQ=255)
                    gagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcct                                                                     >  2:731013/1‑93 (MQ=255)
                    gagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcct                                                                     >  1:3379165/1‑93 (MQ=255)
                      gCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGa                                                                   >  1:4208000/1‑93 (MQ=255)
                      gCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGa                                                                   >  1:3076702/1‑93 (MQ=255)
                       cTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  >  2:6063905/1‑93 (MQ=255)
                       cTTCATGCCTTGACAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  <  2:5721684/93‑1 (MQ=255)
                           aTGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGt                                                              <  1:4447319/93‑1 (MQ=255)
                           aTGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGt                                                              >  1:7325697/1‑93 (MQ=255)
                             gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGa                                                            >  2:6898783/1‑93 (MQ=255)
                             gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGa                                                            <  1:3174083/93‑1 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACGGGCGCTTCCTGTTGTTATGGTATGGTACCACGCTTTTGAGCCTCCTGAATAGTGAt                                                           <  2:7027882/93‑1 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAt                                                           <  1:1002826/93‑1 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAt                                                           <  1:7899972/93‑1 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAt                                                           <  2:4935638/93‑1 (MQ=255)
                                  gagaGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTg                                                       <  1:6008593/93‑1 (MQ=255)
                                    gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGaa                                                     >  2:3786752/1‑93 (MQ=255)
                                     agGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAat                                                    <  2:2167639/93‑1 (MQ=255)
                                     agGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAat                                                    >  2:4356069/1‑93 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  1:260702/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                >  2:3298732/1‑93 (MQ=255)
                                          ctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacat                                               >  2:1399834/1‑93 (MQ=255)
                                            cAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataa                                             >  2:6004888/1‑93 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            <  2:1134943/93‑1 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            <  1:5789870/93‑1 (MQ=255)
                                                tttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcccc                                         >  1:1170336/1‑93 (MQ=255)
                                                 ttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCa                                        >  1:6609492/1‑93 (MQ=255)
                                                 ttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCa                                        <  1:2449766/93‑1 (MQ=255)
                                                 ttCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCa                                        >  1:2120119/1‑93 (MQ=255)
                                                  tCTTTTGCAATGAGACAGGCGCCTCCTGCTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAt                                       <  1:1872600/93‑1 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     <  1:6212907/93‑1 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     >  2:65796/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     >  2:6492442/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     >  1:2764064/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     >  2:534332/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     <  2:4658015/93‑1 (MQ=255)
                                                     tttGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGat                                    <  1:1231302/93‑1 (MQ=255)
                                                      ttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGata                                   <  2:3670294/93‑1 (MQ=255)
                                                       tGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatat                                  <  1:7232054/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 >  1:1287629/1‑93 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  1:413431/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  2:2779371/93‑1 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               <  1:4046079/93‑1 (MQ=255)
                                                           aTGAGCCGGGCGCTTCTTGTTGTAAT‑GTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGa                             <  1:3920003/93‑1 (MQ=255)
                                                            tGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTTAATAACATAACCCCATGATATATCGa                             >  1:845204/1‑93 (MQ=255)
                                                            tGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGa                             <  1:5295494/93‑1 (MQ=255)
                                                            tGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGa                             >  2:6762251/1‑93 (MQ=255)
                                                             gagaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAt                            >  2:6327421/1‑93 (MQ=255)
                                                             gagaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAt                            <  2:5120115/93‑1 (MQ=255)
                                                              agaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATa                           <  2:7093398/93‑1 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                          >  2:4073720/1‑93 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  1:3781822/93‑1 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  1:7489882/93‑1 (MQ=255)
                                                                 cAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaaa                        <  1:2209443/93‑1 (MQ=255)
                                                                  aGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaat                       <  1:3857276/93‑1 (MQ=255)
                                                                   ggCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGAGATGCTGAATAACATAACCCCATGATATATCGATAAaata                      >  1:5578969/1‑93 (MQ=255)
                                                                    gcgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataa                     <  2:7825272/93‑1 (MQ=255)
                                                                    gcgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataa                     <  2:7187954/93‑1 (MQ=255)
                                                                     cgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataat                    <  2:4879476/93‑1 (MQ=255)
                                                                     cgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataat                    <  2:254928/93‑1 (MQ=255)
                                                                     cgcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataat                    <  2:1826942/93‑1 (MQ=255)
                                                                       cTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtct                  >  1:7757543/1‑93 (MQ=255)
                                                                       cTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtct                  >  1:1771193/1‑93 (MQ=255)
                                                                        ttCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtctc                 <  1:5450355/93‑1 (MQ=255)
                                                                        ttCCTGTTGTTATGGTATGGTACCCCGCTATTGCGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtctc                 >  1:6935265/1‑93 (MQ=255)
                                                                         tCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATctct                >  2:305041/1‑93 (MQ=255)
                                                                          tcTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTa               <  1:6855366/92‑1 (MQ=255)
                                                                          ccTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTa               <  1:6653099/93‑1 (MQ=255)
                                                                          ccTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTa               >  1:4535977/1‑93 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            >  2:2960826/1‑93 (MQ=255)
                                                                              ttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAtt           >  1:6987960/1‑93 (MQ=255)
                                                                               tgttATGGTATTGTACCCCGGTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAttt          <  1:1405854/93‑1 (MQ=255)
                                                                                 ttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGa        <  1:4698313/93‑1 (MQ=255)
                                                                                 ttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGa        <  2:1328433/93‑1 (MQ=255)
                                                                                   atggtatggtACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaa      <  2:550604/93‑1 (MQ=255)
                                                                                    tggtatggtaCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaaa     <  2:6716382/93‑1 (MQ=255)
                                                                                    tggtatggtaCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaaa     <  2:2389731/93‑1 (MQ=255)
                                                                                      gTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATg   >  2:1700431/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 231 total aligned reads displayed.
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATG                                                                                               >  SRR4302543.6112058/1‑94 (MQ=60)
 GATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGG                                                                                              <  SRR4302543.7117467/94‑1 (MQ=60)
 GATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGG                                                                                              <  SRR4302543.7169188/94‑1 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGT                                                                                             >  SRR4302543.5349098/1‑94 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGT                                                                                             >  SRR4302543.6136711/1‑94 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGT                                                                                             <  SRR4302543.6451530/94‑1 (MQ=60)
   TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTA                                                                                            >  SRR4302543.1623967/1‑94 (MQ=60)
    TATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAC                                                                                           >  SRR4302543.6986805/1‑94 (MQ=60)
    TATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTAC                                                                                           >  SRR4302543.7534528/1‑94 (MQ=60)
      TTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCC                                                                                         >  SRR4302543.7368382/1‑94 (MQ=60)
       TTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTTCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCC                                                                                        >  SRR4302543.7072420/1‑94 (MQ=60)
       TTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCC                                                                                        >  SRR4302543.7072421/1‑94 (MQ=60)
        TCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCG                                                                                       >  SRR4302543.3830746/1‑94 (MQ=60)
        TCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCC                                                                                       >  SRR4302543.6618170/1‑94 (MQ=60)
         CCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGC                                                                                      >  SRR4302543.2475992/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302543.1468886/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302543.1121065/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     <  SRR4302543.5337611/94‑1 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302543.7890815/1‑94 (MQ=60)
           ATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTA                                                                                    >  SRR4302543.5108168/1‑94 (MQ=60)
            TCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGTGCTTCCTGTTGTTATGGTATGGTACCCCGCTAT                                                                                   <  SRR4302543.6534955/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATNCCTTGAGANGATCTCANTTTTCTTTTGCNATGAGACAGGCGCTTNCTNTTNTNATGGTATGGTACCNNGNTATT                                                                                  >  SRR4302543.3529943/1‑94 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  >  SRR4302543.7213033/1‑94 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.2769879/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.2460192/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.4222027/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.5054958/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.5952969/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302543.5952970/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  >  SRR4302543.7595795/1‑94 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 <  SRR4302543.913/94‑1 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 <  SRR4302543.780622/94‑1 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 >  SRR4302543.7557331/1‑94 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 <  SRR4302543.3517390/94‑1 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                >  SRR4302543.4993689/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                >  SRR4302543.4993691/1‑94 (MQ=60)
               CTTCGGGAGAGCTTCATGCCTTGAGAGGAACGCAAATTTCTCATGCAATGAGACAGGCGCTGCCCGGTGTTAGGGTATGGTACCCCGCGACTGA                                                                                >  SRR4302543.7075787/1‑94 (MQ=21)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302543.3586450/94‑1 (MQ=60)
                    TGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTC                                                                           >  SRR4302543.6150910/1‑94 (MQ=60)
                     GAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCC                                                                          <  SRR4302543.1723633/94‑1 (MQ=60)
                     GAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCC                                                                          >  SRR4302543.3905918/1‑94 (MQ=60)
                      AGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCT                                                                         <  SRR4302543.4723354/94‑1 (MQ=60)
                      AGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCT                                                                         >  SRR4302543.2058198/1‑94 (MQ=60)
                      AGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCT                                                                         >  SRR4302543.3409491/1‑94 (MQ=60)
                       GAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        >  SRR4302543.6993272/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302543.4246153/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302543.6184853/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302543.6676312/1‑94 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       <  SRR4302543.6242758/94‑1 (MQ=60)
                           TTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATA                                                                    >  SRR4302543.1398317/1‑94 (MQ=60)
                            TCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTTTGGTACCCCGCTATTGAGCCTCCTGAATAG                                                                   >  SRR4302543.7889725/1‑94 (MQ=60)
                             CATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGT                                                                  >  SRR4302543.7396229/1‑94 (MQ=60)
                              ATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTG                                                                 <  SRR4302543.4487604/94‑1 (MQ=60)
                              ATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTG                                                                 >  SRR4302543.5935749/1‑94 (MQ=60)
                              ATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTG                                                                 <  SRR4302543.7392541/94‑1 (MQ=60)
                              ATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTG                                                                 >  SRR4302543.2916862/1‑94 (MQ=60)
                               TGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGA                                                                >  SRR4302543.849237/1‑94 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302543.4221963/94‑1 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302543.47423/94‑1 (MQ=60)
                                GCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAT                                                               <  SRR4302543.3202680/94‑1 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              <  SRR4302543.7974235/94‑1 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              <  SRR4302543.1011689/94‑1 (MQ=60)
                                  CTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGC                                                             >  SRR4302543.4491660/1‑94 (MQ=60)
                                  CTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGC                                                             >  SRR4302543.1247240/1‑94 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302543.5224692/94‑1 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302543.4025971/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           <  SRR4302543.192866/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           <  SRR4302543.7041355/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           <  SRR4302543.6882568/94‑1 (MQ=60)
                                     GAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGA                                                          <  SRR4302543.6063951/94‑1 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302543.5096300/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         >  SRR4302543.6656908/1‑94 (MQ=60)
                                       GAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAAT                                                        <  SRR4302543.7472737/94‑1 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       <  SRR4302543.1136107/94‑1 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     >  SRR4302543.1284844/1‑94 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     >  SRR4302543.897710/1‑94 (MQ=60)
                                          GATCTCAATTTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAAC                                                     <  SRR4302543.6586174/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   >  SRR4302543.4170776/1‑94 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   <  SRR4302543.6119793/94‑1 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  <  SRR4302543.4234723/94‑1 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302543.249234/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302543.249233/1‑94 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 <  SRR4302543.7196463/94‑1 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 >  SRR4302543.6006510/1‑94 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGCATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 <  SRR4302543.3079592/94‑1 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                <  SRR4302543.4152297/94‑1 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               >  SRR4302543.4172427/1‑94 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               <  SRR4302543.5842553/94‑1 (MQ=60)
                                                 ATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCC                                              <  SRR4302543.1374652/94‑1 (MQ=60)
                                                 ATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCC                                              >  SRR4302543.3424686/1‑94 (MQ=60)
                                                 ATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCC                                              >  SRR4302543.7463718/1‑94 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             <  SRR4302543.6830508/94‑1 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             >  SRR4302543.1180963/1‑94 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             <  SRR4302543.5627297/94‑1 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             >  SRR4302543.5041899/1‑94 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            >  SRR4302543.6671786/1‑94 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            >  SRR4302543.5601779/1‑94 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            >  SRR4302543.2139099/1‑94 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAT                                           <  SRR4302543.3860359/94‑1 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAT                                           <  SRR4302543.3245839/94‑1 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAT                                           <  SRR4302543.2471861/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.1559121/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.3195872/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.3195871/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.636571/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.1656167/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCCTCCTTTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.2671364/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCCTCCTGCTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302543.1889420/94‑1 (MQ=60)
                                                      CTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGA                                         >  SRR4302543.2789218/1‑94 (MQ=60)
                                                      CTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGA                                         >  SRR4302543.5790486/1‑94 (MQ=60)
                                                       TTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGAT                                        <  SRR4302543.6271567/94‑1 (MQ=60)
                                                       TTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGAT                                        <  SRR4302543.2686259/94‑1 (MQ=60)
                                                        TTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATA                                       <  SRR4302543.6288877/94‑1 (MQ=60)
                                                        TTTGCAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATA                                       <  SRR4302543.1242388/94‑1 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302543.4770672/94‑1 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302543.4700683/94‑1 (MQ=60)
                                                         TGGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302543.3707230/94‑1 (MQ=60)
                                                          TGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTTCTGAATAGTGATGCTGAATAACATAACCCCATGATATA                                     >  SRR4302543.7552568/1‑94 (MQ=60)
                                                          TGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATA                                     >  SRR4302543.7552569/1‑94 (MQ=60)
                                                          TGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATA                                     <  SRR4302543.7302090/94‑1 (MQ=60)
                                                          TGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATA                                     >  SRR4302543.1299104/1‑94 (MQ=60)
                                                           GCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATAT                                    <  SRR4302543.417289/94‑1 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302543.5962526/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302543.1102796/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302543.3334807/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302543.3938685/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302543.4556647/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   <  SRR4302543.3129915/94‑1 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  >  SRR4302543.7033557/1‑94 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  <  SRR4302543.4082287/94‑1 (MQ=60)
                                                              ATGAGCCGGGCGCTTCTTGTTGTAAT‑GTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                <  SRR4302543.3954597/94‑1 (MQ=60)
                                                              ATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTTAATAACATAACCCCATGATATATCGA                                 >  SRR4302543.852691/1‑94 (MQ=60)
                                                               TGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                <  SRR4302543.5343305/94‑1 (MQ=60)
                                                               TGAGACAGGCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                >  SRR4302543.1245457/1‑94 (MQ=60)
                                                                  GACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAA                             >  SRR4302543.4919025/1‑94 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           <  SRR4302543.4223156/94‑1 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           <  SRR4302543.3815133/94‑1 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           <  SRR4302543.7561545/94‑1 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           <  SRR4302543.2229344/94‑1 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           >  SRR4302543.5720090/1‑94 (MQ=60)
                                                                     AGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATA                          >  SRR4302543.6694929/1‑94 (MQ=60)
                                                                     AGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATA                          >  SRR4302543.348396/1‑94 (MQ=60)
                                                                      GGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAA                         <  SRR4302543.5543510/94‑1 (MQ=60)
                                                                       GCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAT                        <  SRR4302543.4924856/94‑1 (MQ=60)
                                                                       GCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAT                        <  SRR4302543.7065308/94‑1 (MQ=60)
                                                                       GCGCCTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAT                        <  SRR4302543.305790/94‑1 (MQ=60)
                                                                        CGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATC                       <  SRR4302543.3909435/94‑1 (MQ=60)
                                                                        CGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATC                       >  SRR4302543.3799995/1‑94 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      <  SRR4302543.4106393/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      <  SRR4302543.1210761/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      >  SRR4302543.6904482/1‑94 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      >  SRR4302543.4227919/1‑94 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      >  SRR4302543.7830762/1‑94 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      >  SRR4302543.1787026/1‑94 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGCGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     >  SRR4302543.7000500/1‑94 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     >  SRR4302543.7202353/1‑94 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCT                    <  SRR4302543.5499789/94‑1 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCT                    <  SRR4302543.726007/94‑1 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCT                    >  SRR4302543.1877518/1‑94 (MQ=60)
                                                                            TCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTA                   <  SRR4302543.1568692/94‑1 (MQ=60)
                                                                            TCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTA                   >  SRR4302543.4868604/1‑94 (MQ=60)
                                                                            TCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTA                   <  SRR4302543.7244866/94‑1 (MQ=60)
                                                                            TCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTA                   >  SRR4302543.4577269/1‑94 (MQ=60)
                                                                             TCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  <  SRR4302543.6920005/94‑1 (MQ=60)
                                                                             CCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  <  SRR4302543.3785869/94‑1 (MQ=60)
                                                                             CCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  <  SRR4302543.6715906/94‑1 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 >  SRR4302543.811411/1‑94 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 <  SRR4302543.7267316/94‑1 (MQ=60)
                                                                               TTTTGTTATGGTATGGAACCCCGCTATTTACCCGCCTGAATAGTGATGCTGAATAACATACCCCAATGATATATCGATAAAATAATCTCTACAT                <  SRR4302543.2207219/94‑1 (MQ=60)
                                                                               TGTTGTTATGGTATGGCACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                <  SRR4302543.2207218/94‑1 (MQ=60)
                                                                                TTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATT               <  SRR4302543.5905998/94‑1 (MQ=60)
                                                                                GTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATCACATAACCCCATGATATATCGATAAAATAATCTCTACATT               >  SRR4302543.1878313/1‑94 (MQ=60)
                                                                                GTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATT               <  SRR4302543.39988/94‑1 (MQ=60)
                                                                                 TTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTT              <  SRR4302543.7838590/94‑1 (MQ=60)
                                                                                 TTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTT              <  SRR4302543.7735356/94‑1 (MQ=60)
                                                                                 TTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTT              >  SRR4302543.2178869/1‑94 (MQ=60)
                                                                                  TGTTATGGTATTGTACCCCGGTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTG             <  SRR4302543.1418547/94‑1 (MQ=60)
                                                                                   GTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGA            >  SRR4302543.6014665/1‑94 (MQ=60)
                                                                                   GTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGA            >  SRR4302543.4314620/1‑94 (MQ=60)
                                                                                   GTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGA            >  SRR4302543.4314621/1‑94 (MQ=60)
                                                                                    TTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAA           <  SRR4302543.1824923/94‑1 (MQ=60)
                                                                                    TTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAA           <  SRR4302543.4741222/94‑1 (MQ=60)
                                                                                     TATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAA          >  SRR4302543.5438927/1‑94 (MQ=60)
                                                                                     TATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACACTTGAAA          <  SRR4302543.2466794/94‑1 (MQ=60)
                                                                                      ATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAA         >  SRR4302543.3819965/1‑94 (MQ=60)
                                                                                      ATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAA         <  SRR4302543.1192565/94‑1 (MQ=60)
                                                                                         GTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC      >  SRR4302543.4566734/1‑94 (MQ=60)
                                                                                         GTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC      <  SRR4302543.5638760/94‑1 (MQ=60)
                                                                                          TATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCA     <  SRR4302543.7045508/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302543.5340477/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302543.4193147/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    >  SRR4302543.631238/1‑94 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    >  SRR4302543.7454067/1‑94 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302543.6661866/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302543.6024925/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302543.6291923/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302543.85784/94‑1 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  SRR4302543.5589038/1‑94 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  SRR4302543.5589039/1‑94 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  <  SRR4302543.6998624/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑