Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,259,162 A→G intergenic (+277/‑86) yjbM → / → yjbN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,259,1620AG99.5% 678.1 / ‑4.9 211intergenic (+277/‑86)yjbM/yjbNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (105/104);  total (106/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 228 total aligned reads displayed.

TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251
                                                                                          |                                                                                         
ttattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTatggtatggt                                                                                         >  1:3204227/1‑93 (MQ=255)
 tattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTAtggtatggta                                                                                        >  1:2391866/1‑93 (MQ=255)
  attTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAc                                                                                       >  1:4071871/1‑93 (MQ=255)
  attTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAc                                                                                       >  1:1831076/1‑93 (MQ=255)
   tttCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAcc                                                                                      >  2:1435130/1‑93 (MQ=255)
    ttCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAccc                                                                                     >  2:3245496/1‑93 (MQ=255)
      ccATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCg                                                                                   <  1:3610887/93‑1 (MQ=255)
        aTCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCt                                                                                 >  2:2895876/1‑93 (MQ=255)
        aTCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCt                                                                                 >  1:1363427/1‑93 (MQ=255)
        aTCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCt                                                                                 <  2:4410604/93‑1 (MQ=255)
        aTCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCt                                                                                 >  2:881378/1‑93 (MQ=255)
          cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAt                                                                               >  2:924329/1‑93 (MQ=255)
          cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAt                                                                               <  1:4016280/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGTCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  2:4565781/1‑93 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              <  2:2993462/93‑1 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  2:662654/1‑93 (MQ=255)
           aCTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTAtt                                                                              >  1:3908977/1‑93 (MQ=255)
            cTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTg                                                                             >  2:141397/1‑93 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            <  1:2371278/93‑1 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            >  2:523883/1‑93 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            >  1:910682/1‑93 (MQ=255)
             ttCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGa                                                                            <  1:1003062/93‑1 (MQ=255)
              tCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAg                                                                           >  1:3789450/1‑93 (MQ=255)
                gTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcc                                                                         >  2:2792898/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  2:2082749/1‑93 (MQ=255)
                 tGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcct                                                                        >  2:2582023/1‑93 (MQ=255)
                   agagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGcctcc                                                                      <  2:901327/93‑1 (MQ=255)
                      gCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGa                                                                   >  1:2472897/1‑93 (MQ=255)
                       cTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  <  2:160612/93‑1 (MQ=255)
                       cTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGaa                                                                  <  1:2339248/93‑1 (MQ=255)
                        ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAt                                                                 >  1:1918397/1‑93 (MQ=255)
                        ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAt                                                                 >  1:4537560/1‑93 (MQ=255)
                         tCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATa                                                                >  2:4281822/1‑93 (MQ=255)
                           aTGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGt                                                              >  1:2901602/1‑93 (MQ=255)
                           aTGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGt                                                              <  1:3375388/93‑1 (MQ=255)
                             gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGa                                                            <  2:3460506/93‑1 (MQ=255)
                              ccTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGAt                                                           <  2:1954699/93‑1 (MQ=255)
                               cTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATg                                                          >  1:4615806/1‑93 (MQ=255)
                                ttGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGc                                                         >  1:1346280/1‑93 (MQ=255)
                                ttGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGc                                                         <  2:770161/93‑1 (MQ=255)
                                   agagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGa                                                      <  1:197562/93‑1 (MQ=255)
                                     agGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAat                                                    <  1:3510072/93‑1 (MQ=255)
                                      ggATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAata                                                   >  2:2979907/1‑93 (MQ=255)
                                       gATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataa                                                  >  2:448345/1‑93 (MQ=255)
                                       gATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataa                                                  <  2:1119566/93‑1 (MQ=255)
                                        aTCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataac                                                 <  1:113554/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  2:2191645/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  1:1551701/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                >  1:3231941/1‑93 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  1:660880/93‑1 (MQ=255)
                                         tctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataaca                                                <  2:2290199/93‑1 (MQ=255)
                                          ctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacat                                               >  1:477588/1‑93 (MQ=255)
                                            cAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataa                                             <  2:3561266/93‑1 (MQ=255)
                                            cAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataa                                             <  1:3646304/93‑1 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            <  1:1655340/93‑1 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            <  2:600383/93‑1 (MQ=255)
                                             aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacataac                                            <  1:3403506/93‑1 (MQ=255)
                                              aTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcc                                           >  1:1208872/1‑93 (MQ=255)
                                              aTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcc                                           <  2:3143345/93‑1 (MQ=255)
                                              aTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcc                                           <  2:4596324/93‑1 (MQ=255)
                                              aTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATACTGAATAACATAAcc                                           >  1:1239970/1‑93 (MQ=255)
                                               ttttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAccc                                          <  2:1774534/93‑1 (MQ=255)
                                                tttCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcccc                                         >  1:4232279/1‑93 (MQ=255)
                                                tttCTTTTACAATGAGACATGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAcccc                                         >  2:4578025/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     >  1:1506666/1‑93 (MQ=255)
                                                    ttttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                     <  1:787662/93‑1 (MQ=255)
                                                     tttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGat                                    <  1:2688226/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  2:3674326/93‑1 (MQ=255)
                                                        gCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatata                                 <  2:250661/93‑1 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               <  1:255333/93‑1 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               <  2:788860/93‑1 (MQ=255)
                                                          aaTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                               >  1:2451317/1‑93 (MQ=255)
                                                           aTGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCg                              >  1:1061354/1‑93 (MQ=255)
                                                            tGAGACAAGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGa                             >  2:4453739/1‑93 (MQ=255)
                                                             gagaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAt                            <  1:1086460/93‑1 (MQ=255)
                                                             gagaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAt                            <  2:433028/93‑1 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                          >  2:721822/1‑93 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                          <  1:698407/93‑1 (MQ=255)
                                                               gaCAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                          <  1:4270024/93‑1 (MQ=255)
                                                                  aGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaat                       <  2:4391960/93‑1 (MQ=255)
                                                                   ggCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaata                      <  1:2953882/93‑1 (MQ=255)
                                                                    gcgcTGCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACACCATGATATATCGATAAaataa                     <  1:2992198/93‑1 (MQ=255)
                                                                      gcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtc                   <  1:157968/93‑1 (MQ=255)
                                                                      gcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtc                   >  1:176078/1‑93 (MQ=255)
                                                                      gcTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtc                   <  2:4014717/93‑1 (MQ=255)
                                                                       cTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtct                  <  1:4105198/93‑1 (MQ=255)
                                                                        ttCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATCATATATCGATAAAATAAtctc                 <  1:4647973/93‑1 (MQ=255)
                                                                        ttCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGAGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtctc                 >  1:3943497/1‑93 (MQ=255)
                                                                         tCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCGCATGATATATCGATAAAATAATctct                >  2:3796515/1‑93 (MQ=255)
                                                                           cTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAc              <  1:2209090/93‑1 (MQ=255)
                                                                           cTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAc              <  2:3846674/93‑1 (MQ=255)
                                                                            tgttgtTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACa             >  2:3993870/1‑93 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            >  2:2321512/1‑93 (MQ=255)
                                                                             gttgttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt            >  1:726785/1‑93 (MQ=255)
                                                                               tgttATCGTATGTCACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATACCATAGCCCCATGATACATCGATAAAACAATCTCTACGttt          <  2:2361012/93‑1 (MQ=255)
                                                                                 ttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGa        <  2:1258618/93‑1 (MQ=255)
                                                                                 ttATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGa        >  1:816151/1‑93 (MQ=255)
                                                                                  tatggtatggTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaa       >  1:2025705/1‑93 (MQ=255)
                                                                                   atggtatggtACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaa      >  2:4070815/1‑93 (MQ=255)
                                                                                    tggtatggtaCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaaa     <  1:1722027/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC  >  NC_000913/4259072‑4259251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATG                                                                                               >  SRR4302535.2959013/1‑94 (MQ=60)
 GATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGG                                                                                              >  SRR4302535.3967365/1‑94 (MQ=60)
  ATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGT                                                                                             >  SRR4302535.3226418/1‑94 (MQ=60)
   TTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTA                                                                                            >  SRR4302535.2409063/1‑94 (MQ=60)
    TATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAC                                                                                           >  SRR4302535.1844290/1‑94 (MQ=60)
    TATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAC                                                                                           >  SRR4302535.4099737/1‑94 (MQ=60)
        TCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCG                                                                                       >  SRR4302535.3460438/1‑94 (MQ=60)
         CCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGC                                                                                      <  SRR4302535.3635928/94‑1 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302535.1373178/1‑94 (MQ=60)
          CATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAAGTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCT                                                                                     >  SRR4302535.3407920/1‑94 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  <  SRR4302535.4043749/94‑1 (MQ=60)
             CACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATT                                                                                  >  SRR4302535.3935749/1‑94 (MQ=60)
              ACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTG                                                                                 >  SRR4302535.227923/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATTGTACCCCCCTATTGG                                                                                >  SRR4302535.254390/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGGTATTGA                                                                                >  SRR4302535.1104426/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                >  SRR4302535.1207755/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                >  SRR4302535.917158/1‑94 (MQ=60)
               CTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGA                                                                                <  SRR4302535.2070995/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               >  SRR4302535.3815586/1‑94 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302535.1010183/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302535.1649007/94‑1 (MQ=60)
                TTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAG                                                                               <  SRR4302535.2388305/94‑1 (MQ=60)
                  CGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCC                                                                             >  SRR4302535.3729463/1‑94 (MQ=60)
                   GTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCT                                                                            >  SRR4302535.1791266/1‑94 (MQ=60)
                   GTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCT                                                                            <  SRR4302535.3909569/94‑1 (MQ=60)
                    TGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTC                                                                           <  SRR4302535.689940/94‑1 (MQ=60)
                     GAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCC                                                                          >  SRR4302535.1972385/1‑94 (MQ=60)
                       GAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        <  SRR4302535.1260237/94‑1 (MQ=60)
                       GAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTG                                                                        <  SRR4302535.4252021/94‑1 (MQ=60)
                        AGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGA                                                                       >  SRR4302535.2490732/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     >  SRR4302535.4568600/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     >  SRR4302535.1932221/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     <  SRR4302535.2355984/94‑1 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     >  SRR4302535.4068434/1‑94 (MQ=60)
                          CTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAAT                                                                     >  SRR4302535.1369524/1‑94 (MQ=60)
                           TTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATA                                                                    <  SRR4302535.473348/94‑1 (MQ=60)
                             CATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGT                                                                  >  SRR4302535.2922113/1‑94 (MQ=60)
                              ATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTG                                                                 <  SRR4302535.3398862/94‑1 (MQ=60)
                               TGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGA                                                                >  SRR4302535.2452872/1‑94 (MQ=60)
                                 CCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATG                                                              >  SRR4302535.4647172/1‑94 (MQ=60)
                                  CTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGC                                                             >  SRR4302535.1355893/1‑94 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302535.4112083/94‑1 (MQ=60)
                                   TTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCT                                                            <  SRR4302535.4042287/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           <  SRR4302535.3653251/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           <  SRR4302535.2674870/94‑1 (MQ=60)
                                    TGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTG                                                           >  SRR4302535.727792/1‑94 (MQ=60)
                                      AGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAA                                                         <  SRR4302535.198920/94‑1 (MQ=60)
                                       GAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAAT                                                        >  SRR4302535.1644153/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       <  SRR4302535.3534463/94‑1 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302535.2166321/1‑94 (MQ=60)
                                        AGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTATTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATA                                                       >  SRR4302535.1407943/1‑94 (MQ=60)
                                         GGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTAACCCGCTATTGCGCCTCCTGAATAGTGATGCTGAATAA                                                      >  SRR4302535.238952/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    >  SRR4302535.1218446/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    >  SRR4302535.3254353/1‑94 (MQ=60)
                                           ATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACA                                                    <  SRR4302535.114348/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   >  SRR4302535.746145/1‑94 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   <  SRR4302535.1562878/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   <  SRR4302535.665491/94‑1 (MQ=60)
                                            TCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACAT                                                   >  SRR4302535.480939/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302535.1678133/1‑94 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  <  SRR4302535.54041/94‑1 (MQ=60)
                                             CTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATA                                                  >  SRR4302535.1929081/1‑94 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 <  SRR4302535.1856595/94‑1 (MQ=60)
                                              TCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAA                                                 >  SRR4302535.1538004/1‑94 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                <  SRR4302535.3671555/94‑1 (MQ=60)
                                               CAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAAC                                                >  SRR4302535.4302877/1‑94 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               >  SRR4302535.1217560/1‑94 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               <  SRR4302535.1667313/94‑1 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               <  SRR4302535.3244816/94‑1 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACC                                               <  SRR4302535.3427193/94‑1 (MQ=60)
                                                AATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATACTGAATAACATAACC                                               >  SRR4302535.1248821/1‑94 (MQ=60)
                                                  TTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCC                                             >  SRR4302535.4261243/1‑94 (MQ=60)
                                                   TTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCA                                            <  SRR4302535.1087388/94‑1 (MQ=60)
                                                    TTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCAT                                           <  SRR4302535.337683/94‑1 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGACTAGTGATGCTGAATAACATAACCCCATG                                          >  SRR4302535.1062388/1‑94 (MQ=60)
                                                     TCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATG                                          <  SRR4302535.3115272/94‑1 (MQ=60)
                                                      CTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGA                                         >  SRR4302535.2589833/1‑94 (MQ=60)
                                                      CTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGA                                         >  SRR4302535.1517498/1‑94 (MQ=60)
                                                       TTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGAT                                        <  SRR4302535.793189/94‑1 (MQ=60)
                                                        TTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATA                                       <  SRR4302535.2707437/94‑1 (MQ=60)
                                                         TTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATAT                                      <  SRR4302535.3224111/94‑1 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302535.2468985/1‑94 (MQ=60)
                                                            CAATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATC                                   >  SRR4302535.3432197/1‑94 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  <  SRR4302535.257093/94‑1 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  >  SRR4302535.1461618/1‑94 (MQ=60)
                                                             AATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCG                                  >  SRR4302535.1068911/1‑94 (MQ=60)
                                                              ATGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGA                                 >  SRR4302535.999546/1‑94 (MQ=60)
                                                               TGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                >  SRR4302535.3954946/1‑94 (MQ=60)
                                                               TGAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGAT                                <  SRR4302535.2015909/94‑1 (MQ=60)
                                                                GAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATA                               <  SRR4302535.1094184/94‑1 (MQ=60)
                                                                GAGACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATA                               <  SRR4302535.715866/94‑1 (MQ=60)
                                                                  GACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAA                             <  SRR4302535.4299258/94‑1 (MQ=60)
                                                                  GACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAA                             <  SRR4302535.703308/94‑1 (MQ=60)
                                                                   ACAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAA                            <  SRR4302535.4281684/94‑1 (MQ=60)
                                                                    CAGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAAT                           >  SRR4302535.4650621/1‑94 (MQ=60)
                                                                     AGGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATA                          <  SRR4302535.4701169/94‑1 (MQ=60)
                                                                      GGCGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAA                         <  SRR4302535.2974631/94‑1 (MQ=60)
                                                                       GCGCTGCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACACCATGATATATCGATAAAATAAT                        <  SRR4302535.3013178/94‑1 (MQ=60)
                                                                        CGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATC                       >  SRR4302535.177303/1‑94 (MQ=60)
                                                                        CGCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATC                       <  SRR4302535.4272224/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      >  SRR4302535.300046/1‑94 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      <  SRR4302535.159084/94‑1 (MQ=60)
                                                                         GCTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCT                      <  SRR4302535.887619/94‑1 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     <  SRR4302535.4133304/94‑1 (MQ=60)
                                                                          CTTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGAGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTC                     >  SRR4302535.3970420/1‑94 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCT                    >  SRR4302535.3609803/1‑94 (MQ=60)
                                                                           TTCCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATCATATATCGATAAAATAATCTCT                    <  SRR4302535.4679457/94‑1 (MQ=60)
                                                                             CCTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAC                  <  SRR4302535.4620618/94‑1 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 <  SRR4302535.2224825/94‑1 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 <  SRR4302535.2954125/94‑1 (MQ=60)
                                                                              CTGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACA                 <  SRR4302535.2361951/94‑1 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                <  SRR4302535.2429166/94‑1 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                >  SRR4302535.15852/1‑94 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                <  SRR4302535.4063020/94‑1 (MQ=60)
                                                                               TGTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAT                >  SRR4302535.731889/1‑94 (MQ=60)
                                                                                GTTGTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATT               <  SRR4302535.248965/94‑1 (MQ=60)
                                                                                   GTTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGA            >  SRR4302535.821874/1‑94 (MQ=60)
                                                                                    TTATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAA           >  SRR4302535.2040303/1‑94 (MQ=60)
                                                                                      ATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAA         <  SRR4302535.2892215/94‑1 (MQ=60)
                                                                                      ATGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAA         <  SRR4302535.802003/94‑1 (MQ=60)
                                                                                       TGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAAT        <  SRR4302535.1734483/94‑1 (MQ=60)
                                                                                       TGGTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAAT        <  SRR4302535.175254/94‑1 (MQ=60)
                                                                                         GTATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGC      <  SRR4302535.1200325/94‑1 (MQ=60)
                                                                                          TATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCA     <  SRR4302535.3744676/94‑1 (MQ=60)
                                                                                           ATGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAC    <  SRR4302535.3759655/94‑1 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATCAAATAAACTCTACCTTTGAAAATGCACG   >  SRR4302535.3234756/1‑94 (MQ=60)
                                                                                            TGGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACG   <  SRR4302535.3953369/94‑1 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  <  SRR4302535.2236050/94‑1 (MQ=60)
                                                                                             GGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  SRR4302535.2113708/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGG  >  NC_000913/4259069‑4259255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑