Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,212 A→G R413R (CGT→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2120AG100.0% 650.5 / NA 209R413R (CGT→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (116/93);  total (116/93)

Only 100 of 226 total aligned reads displayed.

GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC  >  NC_000913/4270122‑4270300
                                                                                          |                                                                                        
gaagaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGcg                                                                                        >  1:1455197/1‑93 (MQ=255)
   gaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGt                                                                                     >  1:853910/1‑93 (MQ=255)
   gaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGt                                                                                     >  2:1844645/1‑93 (MQ=255)
    aaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTc                                                                                    >  2:3162414/1‑93 (MQ=255)
       tCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCt                                                                                 <  1:1531557/93‑1 (MQ=255)
       tCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCt                                                                                 >  2:2569069/1‑93 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                >  1:4202224/1‑93 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                >  1:1935115/1‑93 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                               <  1:3575332/93‑1 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                               <  2:1698972/93‑1 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                               >  2:2431838/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                              >  2:4501970/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                              >  2:3438888/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                              >  1:2464487/1‑93 (MQ=255)
           tCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGc                                                                             <  1:3889351/93‑1 (MQ=255)
            cGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCa                                                                            <  2:1191006/93‑1 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                           >  2:3902441/1‑93 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                           >  1:3326898/1‑93 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                          >  2:4031550/1‑93 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                        >  2:3733645/1‑93 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                        >  1:2081414/1‑93 (MQ=255)
                 gACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTgg                                                                       <  2:4250794/93‑1 (MQ=255)
                  aCCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCCTCGCACCTGgc                                                                      >  2:2478132/1‑93 (MQ=255)
                   ccAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcg                                                                     >  2:2658917/1‑93 (MQ=255)
                   ccAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcg                                                                     >  1:4122025/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgc                                                                    <  2:91826/93‑1 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                   >  2:180279/1‑92 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                   <  1:3617760/93‑2 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                   >  1:1241221/1‑92 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                   >  2:2779908/1‑92 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgc                                                                  >  1:3436208/1‑91 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgc                                                                  >  1:653058/1‑91 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                               >  1:4653620/1‑93 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                               >  2:957658/1‑93 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                               >  1:3910642/1‑93 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                               <  1:2024563/93‑1 (MQ=255)
                         cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                               <  1:2426217/93‑1 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                              >  1:3804249/1‑93 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                              >  1:1608556/1‑93 (MQ=255)
                          tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                              >  1:2666594/1‑93 (MQ=255)
                           cATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTTGCGCGCGGGCg                                                             >  2:113789/1‑93 (MQ=255)
                           cATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACg                                                             >  1:1772004/1‑93 (MQ=255)
                           cATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACg                                                             >  1:3299265/1‑93 (MQ=255)
                             tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                           <  2:3348930/93‑1 (MQ=255)
                               tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                         >  1:2369215/1‑93 (MQ=255)
                                 ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                       >  2:3053132/1‑93 (MQ=255)
                                 ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                       >  1:2998580/1‑93 (MQ=255)
                                   agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                     >  2:3136846/1‑93 (MQ=255)
                                    gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                    <  1:4361500/93‑1 (MQ=255)
                                       aGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaaa                                                 >  2:579334/1‑93 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                <  1:4338722/93‑1 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                >  1:3639006/1‑93 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                <  2:1903426/93‑1 (MQ=255)
                                         caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                               <  1:979202/93‑1 (MQ=255)
                                         caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                               >  1:495061/1‑93 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                              >  2:2943012/1‑93 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                              <  2:209886/93‑1 (MQ=255)
                                           ggcagCGGCGTATTCTCGACTTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                             <  1:3128048/93‑1 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATGCACGTGGCGCGCTGCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                             <  2:447138/93‑1 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                             <  1:3709306/93‑1 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                             >  2:2744824/1‑93 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                       >  1:2589731/1‑93 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                       >  1:3142457/1‑93 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                       <  2:319744/93‑1 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                       <  2:2015672/93‑1 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                      <  2:1326798/93‑1 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                      >  2:3518679/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                      >  1:4523579/1‑93 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCTGGCGCGACACGTCGCAGCTGGCGCGCGTTCGATTCATGAGAGACTTGGCTAGttctc                                   >  2:4439274/1‑92 (MQ=255)
                                                     tATCCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                   <  2:3264527/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                  <  1:2110916/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                  >  2:4199159/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                  <  2:409552/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                  >  2:4600828/1‑93 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttttttg                                 >  2:860379/1‑91 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                 >  1:3473605/1‑93 (MQ=255)
                                                       ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                 <  2:2033547/93‑1 (MQ=255)
                                                        tctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgc                                <  2:1736293/93‑1 (MQ=255)
                                                          tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                              <  2:716042/93‑1 (MQ=255)
                                                            gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                            >  1:1598363/1‑93 (MQ=255)
                                                             aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                           <  1:4236755/93‑1 (MQ=255)
                                                             aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                           >  2:3582624/1‑93 (MQ=255)
                                                             aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                           <  1:3759922/93‑1 (MQ=255)
                                                              cATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcg                          >  2:1171109/1‑93 (MQ=255)
                                                                 acacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCgcgc                       >  1:732133/1‑93 (MQ=255)
                                                                   acGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTg                     <  2:1457602/93‑1 (MQ=255)
                                                                        gcgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                <  2:1929870/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc            <  1:1030227/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc            <  1:544857/93‑1 (MQ=255)
                                                                              ctCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc          <  1:1250244/92‑1 (MQ=255)
                                                                               tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct         >  1:2323751/1‑93 (MQ=255)
                                                                               tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct         <  1:2898226/93‑1 (MQ=255)
                                                                               tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct         <  2:1632612/93‑1 (MQ=255)
                                                                                cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt        <  2:377901/93‑1 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta      <  1:4018941/93‑1 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat     >  2:494731/1‑93 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat     <  1:2771911/93‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata    <  2:566024/93‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata    >  1:4477754/1‑93 (MQ=255)
                                                                                     cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAg   <  2:2293481/93‑1 (MQ=255)
                                                                                          |                                                                                        
GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC  >  NC_000913/4270122‑4270300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303
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GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGG                                                                                             >  SRR4302535.111288/1‑94 (MQ=60)
 TTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGC                                                                                            <  SRR4302535.872225/94‑1 (MQ=60)
 TTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGC                                                                                            <  SRR4302535.1863066/94‑1 (MQ=60)
  TGAAGAATTGCATCGCACGACCAATGGTCATGCCGTAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           <  SRR4302535.719357/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           <  SRR4302535.2191061/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           <  SRR4302535.408369/94‑1 (MQ=60)
  TGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCG                                                                                           >  SRR4302535.1465611/1‑94 (MQ=60)
   GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGC                                                                                          >  SRR4302535.3779897/1‑94 (MQ=60)
    AAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCG                                                                                         <  SRR4302535.841142/94‑1 (MQ=60)
    AAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCG                                                                                         >  SRR4302535.3662744/1‑94 (MQ=60)
     AGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGT                                                                                        >  SRR4302535.859924/1‑94 (MQ=60)
         TTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCT                                                                                    >  SRR4302535.2012861/1‑94 (MQ=60)
         TTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCT                                                                                    >  SRR4302535.599950/1‑94 (MQ=60)
         TTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCT                                                                                    >  SRR4302535.4213571/1‑94 (MQ=60)
         TTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGAGCGTCCCT                                                                                    >  SRR4302535.1251920/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   <  SRR4302535.1542593/94‑1 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   >  SRR4302535.4230974/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   >  SRR4302535.1949052/1‑94 (MQ=60)
          TCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTT                                                                                   >  SRR4302535.3974454/1‑94 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 >  SRR4302535.2482257/1‑94 (MQ=60)
            CATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCG                                                                                 <  SRR4302535.3600119/94‑1 (MQ=60)
             ATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGC                                                                                >  SRR4302535.3930872/1‑94 (MQ=60)
             ATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGC                                                                                >  SRR4302535.4601696/1‑94 (MQ=60)
              TCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCA                                                                               <  SRR4302535.3916020/94‑1 (MQ=60)
              TCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCA                                                                               <  SRR4302535.378636/94‑1 (MQ=60)
               CGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAG                                                                              >  SRR4302535.3350050/1‑94 (MQ=60)
               CGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAG                                                                              >  SRR4302535.1060154/1‑94 (MQ=60)
                  ATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCGAACGAAGGCGCGTCCCTTCGCAGCTG                                                                           >  SRR4302535.1318566/1‑94 (MQ=60)
                  ATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTG                                                                           >  SRR4302535.467610/1‑94 (MQ=60)
                  ATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTG                                                                           >  SRR4302535.2096409/1‑94 (MQ=60)
                     ACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCG                                                                        >  SRR4302535.4150232/1‑94 (MQ=60)
                       CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                      >  SRR4302535.1250082/1‑94 (MQ=60)
                       CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                      <  SRR4302535.53893/94‑1 (MQ=60)
                        AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                     <  SRR4302535.3642840/94‑1 (MQ=60)
                        AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                     >  SRR4302535.3460089/1‑94 (MQ=60)
                        AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                     >  SRR4302535.657614/1‑94 (MQ=60)
                         ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                    <  SRR4302535.3150798/94‑1 (MQ=60)
                           GCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGA                                                                  >  SRR4302535.4685125/1‑94 (MQ=60)
                           GCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGA                                                                  >  SRR4302535.3937418/1‑94 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 <  SRR4302535.2039152/94‑1 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 <  SRR4302535.4686713/94‑1 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302535.2685682/1‑94 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302535.1620157/1‑94 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 <  SRR4302535.2443697/94‑1 (MQ=60)
                            CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                 >  SRR4302535.3830473/1‑94 (MQ=60)
                             TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                >  SRR4302535.275290/1‑94 (MQ=60)
                             TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                >  SRR4302535.1784845/1‑94 (MQ=60)
                             TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                >  SRR4302535.3322211/1‑94 (MQ=60)
                              CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGGGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                               <  SRR4302535.153360/94‑1 (MQ=60)
                               ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                              >  SRR4302535.3338559/1‑94 (MQ=60)
                                 GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                            >  SRR4302535.2386224/1‑94 (MQ=60)
                                  TCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCTCGGACGATTAC                                                           <  SRR4302535.4278700/94‑1 (MQ=60)
                                  TCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                           <  SRR4302535.3670901/94‑1 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCTCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          <  SRR4302535.2398884/94‑1 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          >  SRR4302535.3019606/1‑94 (MQ=60)
                                   CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                          >  SRR4302535.2334579/1‑94 (MQ=60)
                                      AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                       >  SRR4302535.3336505/1‑94 (MQ=60)
                                       GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                      <  SRR4302535.665446/94‑1 (MQ=60)
                                       GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                      <  SRR4302535.4391349/94‑1 (MQ=60)
                                         AAGCAGGCAGCGGCGTATTCTCAACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAA                                                    <  SRR4302535.286481/94‑1 (MQ=60)
                                          AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   <  SRR4302535.2977595/94‑1 (MQ=60)
                                          AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                   >  SRR4302535.3664218/1‑94 (MQ=60)
                                           GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  >  SRR4302535.498534/1‑94 (MQ=60)
                                           GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                  <  SRR4302535.4368391/94‑1 (MQ=60)
                                            CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                 <  SRR4302535.986163/94‑1 (MQ=60)
                                            CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                 >  SRR4302535.2375600/1‑94 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACTTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302535.3149832/94‑1 (MQ=60)
                                              GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                               <  SRR4302535.3734996/94‑1 (MQ=60)
                                               GCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGC                                              <  SRR4302535.3977409/94‑1 (MQ=60)
                                                CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                             <  SRR4302535.3529460/94‑1 (MQ=60)
                                                 AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                            <  SRR4302535.4528376/94‑1 (MQ=60)
                                                  GCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAA                                           <  SRR4302535.4379507/94‑1 (MQ=60)
                                                   CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                          >  SRR4302535.2608327/1‑94 (MQ=60)
                                                   CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                          >  SRR4302535.3164322/1‑94 (MQ=60)
                                                   CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                          <  SRR4302535.880477/94‑1 (MQ=60)
                                                   CGGCGAATTCTCGACATCCACGTGGCGCCCTCCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA‑TACTGATAAACTTGGCTAATT                                         <  SRR4302535.722945/94‑1 (MQ=60)
                                                    GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                         >  SRR4302535.4554571/1‑94 (MQ=60)
                                                     GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                        <  SRR4302535.2229156/94‑1 (MQ=60)
                                                       GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                      <  SRR4302535.2513107/94‑1 (MQ=60)
                                                       GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                      >  SRR4302535.4058057/1‑94 (MQ=60)
                                                         ATTCTCGACATGCACGTGGCGCGGTTCCCGCCGCAGGCGCCTCGCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302535.296753/94‑1 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302535.2115049/94‑1 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    >  SRR4302535.3497740/1‑94 (MQ=60)
                                                         ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                    <  SRR4302535.2126142/94‑1 (MQ=60)
                                                           TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                  <  SRR4302535.3628460/94‑1 (MQ=60)
                                                           TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                  <  SRR4302535.186874/94‑1 (MQ=60)
                                                              CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                               >  SRR4302535.1609892/1‑94 (MQ=60)
                                                               GACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACC                              >  SRR4302535.4291024/1‑94 (MQ=60)
                                                                ACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                             <  SRR4302535.3785910/94‑1 (MQ=60)
                                                                ACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                             <  SRR4302535.4265749/94‑1 (MQ=60)
                                                                 CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                            >  SRR4302535.3468255/1‑94 (MQ=60)
                                                                   TACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                          >  SRR4302535.737273/1‑94 (MQ=60)
                                                                       CGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCCCGTACCGCGCTGGT                      >  SRR4302535.31325/1‑94 (MQ=60)
                                                                        GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                     <  SRR4302535.2685637/94‑1 (MQ=60)
                                                                          GGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTC                   <  SRR4302535.4442142/94‑1 (MQ=60)
                                                                           GCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCC                  <  SRR4302535.2462875/94‑1 (MQ=60)
                                                                             GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                >  SRR4302535.3458231/1‑94 (MQ=60)
                                                                             GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                >  SRR4302535.3763533/1‑94 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              <  SRR4302535.1037540/94‑1 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              <  SRR4302535.1513691/94‑1 (MQ=60)
                                                                               GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT              <  SRR4302535.548663/94‑1 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             >  SRR4302535.1700922/1‑94 (MQ=60)
                                                                                CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC             >  SRR4302535.1590488/1‑94 (MQ=60)
                                                                                 TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            >  SRR4302535.2340378/1‑94 (MQ=60)
                                                                                 CTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT            <  SRR4302535.1259170/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302535.2918723/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302535.3838189/94‑1 (MQ=60)
                                                                                  TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT           <  SRR4302535.3265351/94‑1 (MQ=60)
                                                                                   CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT          <  SRR4302535.3584207/94‑1 (MQ=60)
                                                                                     CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT        <  SRR4302535.4046439/94‑1 (MQ=60)
                                                                                     CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT        <  SRR4302535.2599203/94‑1 (MQ=60)
                                                                                     CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT        >  SRR4302535.4240241/1‑94 (MQ=60)
                                                                                      GACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA       <  SRR4302535.2791650/94‑1 (MQ=60)
                                                                                      GACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA       >  SRR4302535.4508498/1‑94 (MQ=60)
                                                                                       ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG      <  SRR4302535.1885315/94‑1 (MQ=60)
                                                                                         GCAGGCGCGTCCGTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG    <  SRR4302535.16162/94‑1 (MQ=60)
                                                                                         GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG    <  SRR4302535.204256/94‑1 (MQ=60)
                                                                                           AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  SRR4302535.1116707/1‑94 (MQ=60)
                                                                                             |                                                                                           
GTTGAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC  >  NC_000913/4270119‑4270303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑