Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,235 A→G C406R (TGC→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2350AG99.7% 1064.6 / ‑5.8 345C406R (TGC→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (154/189);  total (155/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 361 total aligned reads displayed.

TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324
                                                                                          |                                                                                         
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  1:3754470/1‑93 (MQ=255)
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  2:6663549/1‑93 (MQ=255)
 gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCgg                                                                                        >  1:431855/1‑93 (MQ=255)
  cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                                                       >  1:4366124/1‑93 (MQ=255)
  cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                                                       <  1:2492526/93‑1 (MQ=255)
  cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                                                       <  2:2397248/93‑1 (MQ=255)
   ccATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      <  2:2858028/92‑1 (MQ=255)
     aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    <  1:5387728/93‑1 (MQ=255)
      tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                                                   >  2:3138870/1‑93 (MQ=255)
       gTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAtt                                                                                  >  1:4809725/1‑93 (MQ=255)
       gTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAtt                                                                                  <  2:7742109/93‑1 (MQ=255)
        gcGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                                                 <  1:810704/92‑1 (MQ=255)
        gcGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                                                 <  2:5776774/92‑1 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               <  2:3350655/93‑1 (MQ=255)
           gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTg                                                                              >  1:176461/1‑93 (MQ=255)
           gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTg                                                                              <  1:7128809/93‑1 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             >  1:231752/1‑93 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             >  2:7656033/1‑93 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            >  2:5468854/1‑93 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            >  1:5849821/1‑93 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  2:6521919/1‑93 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  1:7211059/1‑93 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  1:4596123/1‑93 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  1:6077606/1‑93 (MQ=255)
               tAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaa                                                                          >  2:6367452/1‑93 (MQ=255)
               tAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaa                                                                          >  2:1991171/1‑93 (MQ=255)
                      cagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGc                                                                   <  1:12887/93‑1 (MQ=255)
                        gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                                                 <  1:5103449/93‑1 (MQ=255)
                        gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                                                 >  1:5022864/1‑93 (MQ=255)
                        gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                                                 >  1:3495828/1‑93 (MQ=255)
                         cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                                                <  1:7286344/93‑1 (MQ=255)
                          ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                                               <  2:138992/93‑1 (MQ=255)
                          ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                                               <  2:4011233/93‑1 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              <  1:339240/93‑1 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  2:3887725/1‑93 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            >  2:568876/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  1:745519/1‑93 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:5178010/93‑1 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       <  1:3920838/93‑1 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  1:425159/1‑93 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  2:2253202/1‑93 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  1:3596209/1‑93 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  1:5336488/1‑93 (MQ=255)
                                    cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                                                     <  2:1216524/93‑1 (MQ=255)
                                    cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                                                     >  2:483800/1‑93 (MQ=255)
                                    cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                                                     <  2:4100911/93‑1 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   <  2:6112063/93‑1 (MQ=255)
                                       cATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAccc                                                  >  2:4456703/1‑92 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTATTCGCGTACcgcg                                                >  2:1828990/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  1:2932444/1‑93 (MQ=255)
                                           cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                                              <  2:4509004/93‑1 (MQ=255)
                                           cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                                              <  1:7743967/93‑1 (MQ=255)
                                           cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                                              <  1:6951768/93‑1 (MQ=255)
                                            acGTGGCGCGCTTCCCGACGCAGGCGCGTTCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTg                                             >  2:3488568/1‑93 (MQ=255)
                                              gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                                           <  1:2471318/93‑1 (MQ=255)
                                              gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                                           <  1:6632717/93‑1 (MQ=255)
                                                 gcgcgcTTCCCGACGCAGGCGCGTCGCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                                        <  1:7077858/93‑1 (MQ=255)
                                                 gcgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                                        <  2:7396359/93‑1 (MQ=255)
                                                    cgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGt                                     >  1:6469487/1‑93 (MQ=255)
                                                     gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc                                    <  1:113254/93‑1 (MQ=255)
                                                     gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc                                    >  1:1049686/1‑93 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   >  2:7860080/1‑93 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   <  1:7824820/93‑1 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  >  2:2055546/1‑93 (MQ=255)
                                                        tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  1:4686506/93‑1 (MQ=255)
                                                        tCCCGACGCAGGCGCGTCCCTTCGCAGCTGCCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  1:216863/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               >  1:2795052/1‑93 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  1:5569421/93‑1 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              <  2:5097268/93‑1 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATCCCGTCTCTTta                              <  2:7804423/93‑1 (MQ=255)
                                                            gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat                             >  2:72178/1‑93 (MQ=255)
                                                             aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata                            <  1:6816219/93‑1 (MQ=255)
                                                               gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc                          <  2:1292656/93‑1 (MQ=255)
                                                               gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc                          <  1:6566292/93‑1 (MQ=255)
                                                                cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg                         <  1:6338473/93‑1 (MQ=255)
                                                                cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg                         >  2:421798/1‑93 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    <  1:3134493/93‑1 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  1:1385851/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  2:5565292/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  1:4377201/1‑93 (MQ=255)
                                                                        gcccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 <  1:271885/92‑1 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGTACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  2:1632905/93‑1 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  2:4534173/93‑1 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  2:3723327/93‑1 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  2:2565441/93‑1 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              >  1:2017594/1‑93 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              <  1:5185396/93‑1 (MQ=255)
                                                                            ttCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatt             >  1:4071942/1‑93 (MQ=255)
                                                                            ttCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatt             >  2:1047283/1‑93 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            <  1:2237433/93‑1 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            <  1:1526035/93‑1 (MQ=255)
                                                                               gCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCTGCGCTCCAtattatc          >  1:6676928/1‑92 (MQ=255)
                                                                               gCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATg          <  2:2621632/93‑1 (MQ=255)
                                                                               gCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATg          >  2:7095947/1‑93 (MQ=255)
                                                                               gCAGCTGGCGCCCCGTCGATTACTGATAAAATTCGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATg          >  2:1148182/1‑93 (MQ=255)
                                                                                  gCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcag       >  1:4133703/1‑93 (MQ=255)
                                                                                  gCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcag       <  2:681779/93‑1 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     >  1:907375/1‑93 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     <  1:548926/93‑1 (MQ=255)
                                                                                      gcgcgcGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACg   >  2:2949183/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 213 total aligned reads displayed.
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328
                                                                                             |                                                                                             
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               >  SRR4302543.2645471/1‑94 (MQ=60)
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               >  SRR4302543.4372098/1‑94 (MQ=60)
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               <  SRR4302543.6916925/94‑1 (MQ=60)
 AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                                              >  SRR4302543.2147519/1‑94 (MQ=60)
 AAGGCTGATGTCGGAGAGAGCAGGCAGCGGCTTATTCTCGACATACACGTGGCGCGCTCCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                                              <  SRR4302543.3744113/94‑1 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302543.322941/94‑1 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             >  SRR4302543.3787502/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             >  SRR4302543.5431527/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302543.5711903/94‑1 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGTGCGCTTCCCGACGCCGGCGCGTCCCTTCGCAGCTGGCGCTCGG                                                                                            >  SRR4302543.7511655/1‑94 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            >  SRR4302543.7511653/1‑94 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            >  SRR4302543.435901/1‑94 (MQ=60)
    GCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGA                                                                                           >  SRR4302543.4405673/1‑94 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          >  SRR4302543.1064736/1‑94 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302543.3124209/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302543.2515125/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          >  SRR4302543.1765425/1‑94 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302543.6722958/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302543.1552500/94‑1 (MQ=60)
     CTCATGGCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302543.1807764/94‑1 (MQ=60)
      TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                                         >  SRR4302543.3866079/1‑94 (MQ=60)
       CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                                                        >  SRR4302543.3113734/1‑94 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302543.5436676/94‑1 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302543.7403878/94‑1 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302543.4322230/94‑1 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302543.4410895/94‑1 (MQ=60)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      <  SRR4302543.6502637/94‑1 (MQ=60)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      <  SRR4302543.1283030/94‑1 (MQ=60)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      >  SRR4302543.5308918/1‑94 (MQ=60)
          GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                                                     <  SRR4302543.7027167/94‑1 (MQ=60)
           TCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                                                    <  SRR4302543.2497938/94‑1 (MQ=60)
           GCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                                                    <  SRR4302543.817845/94‑1 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302543.7829133/94‑1 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  >  SRR4302543.177972/1‑94 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  >  SRR4302543.5221931/1‑94 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302543.2206961/94‑1 (MQ=60)
              GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGA                                                                                 <  SRR4302543.4407884/94‑1 (MQ=60)
              GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGA                                                                                 >  SRR4302543.233753/1‑94 (MQ=60)
              GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGA                                                                                 <  SRR4302543.7198085/94‑1 (MQ=60)
               GGATAGCGGGCGGCGGCGTATTCTCGCCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTTCTGAT                                                                                <  SRR4302543.7069777/94‑1 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302543.5238958/94‑1 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                >  SRR4302543.5903051/1‑94 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302543.4637861/1‑94 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302543.7280962/1‑94 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               <  SRR4302543.661014/94‑1 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302543.4637860/1‑94 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302543.6135209/1‑94 (MQ=60)
                  TAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAA                                                                             <  SRR4302543.5560515/94‑1 (MQ=60)
                   AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            >  SRR4302543.897624/1‑94 (MQ=60)
                   AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            <  SRR4302543.5018243/94‑1 (MQ=60)
                   AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            >  SRR4302543.1160500/1‑94 (MQ=60)
                     CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                                          >  SRR4302543.3121437/1‑94 (MQ=60)
                     CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                                          <  SRR4302543.6918394/94‑1 (MQ=60)
                      AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                                         <  SRR4302543.5744887/94‑1 (MQ=60)
                      AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                                         >  SRR4302543.3847143/1‑94 (MQ=60)
                      AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGACTACTGATAAACTTG                                                                         >  SRR4302543.6410728/1‑94 (MQ=60)
                       GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACTATTACTGATAAACTTGG                                                                        <  SRR4302543.7800442/94‑1 (MQ=60)
                        GCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGC                                                                       <  SRR4302543.1402233/94‑1 (MQ=60)
                        GCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGC                                                                       <  SRR4302543.4989396/94‑1 (MQ=60)
                         CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                                                      <  SRR4302543.13000/94‑1 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302543.5068574/1‑94 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302543.3527106/1‑94 (MQ=60)
                           GCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAA                                                                    <  SRR4302543.5149642/94‑1 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGCCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTCCTGATATACTTGGCTAAT                                                                   >  SRR4302543.5940029/1‑94 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                                                   <  SRR4302543.422292/94‑1 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                                                   <  SRR4302543.7356690/94‑1 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302543.6720889/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  <  SRR4302543.3759899/94‑1 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTGGCGCACGGACGATTACTGATAAACCTGGCTAATTT                                                                 >  SRR4302543.4325257/1‑94 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTTGCTAATTT                                                                 >  SRR4302543.5745805/1‑94 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 <  SRR4302543.342358/94‑1 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 <  SRR4302543.2642104/94‑1 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 <  SRR4302543.2916369/94‑1 (MQ=60)
                               CGTATTCTCGACATACGCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302543.2338244/94‑1 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302543.6858464/94‑1 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302543.1073068/94‑1 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302543.955303/1‑94 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302543.3691461/94‑1 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302543.7683054/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302543.2940509/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302543.752152/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302543.580283/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTG                                                               >  SRR4302543.1411121/1‑94 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302543.5516065/1‑94 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302543.4735877/1‑94 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302543.5258206/1‑94 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              <  SRR4302543.6920446/94‑1 (MQ=60)
                                 GATTCTCGACATACACGTGGCGCGCCTCCCGGCGCAGGCCGGTCCCTTCCCAACTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              <  SRR4302543.5358600/94‑1 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302543.5611090/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302543.6562553/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302543.3459067/1‑94 (MQ=60)
                                    TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                                           <  SRR4302543.5218309/94‑1 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          >  SRR4302543.429141/1‑94 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          <  SRR4302543.3955440/94‑1 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          >  SRR4302543.5384752/1‑94 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          >  SRR4302543.3627950/1‑94 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        <  SRR4302543.2837085/94‑1 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        >  SRR4302543.5139176/1‑94 (MQ=60)
                                        GACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACC                                                       <  SRR4302543.5529904/94‑1 (MQ=60)
                                         ACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                                                      >  SRR4302543.4389643/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302543.2959073/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302543.7980758/1‑94 (MQ=60)
                                            TACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                                                   <  SRR4302543.1310122/94‑1 (MQ=60)
                                              CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                                                 <  SRR4302543.7817112/94‑1 (MQ=60)
                                              CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                                                 <  SRR4302543.7017166/94‑1 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302543.6695297/94‑1 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302543.2493627/94‑1 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              >  SRR4302543.2171918/1‑94 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              >  SRR4302543.2666479/1‑94 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTACGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302543.7230037/94‑1 (MQ=60)
                                                  GGGCGCGCTTCCCAACGCAGGCGCGTCCCTTCGCAGCTGGCACGCAGACGATTACTGATAAACTTGGCTAATTATTCGCGTACCGCGATGGATT                                             <  SRR4302543.7099963/94‑1 (MQ=60)
                                                   GGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTC                                            >  SRR4302543.6892983/1‑94 (MQ=60)
                                                   GGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTC                                            <  SRR4302543.4174610/94‑1 (MQ=60)
                                                    GCGCGCTTCCCGACGCAGGCGCGTCGCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCC                                           <  SRR4302543.7146594/94‑1 (MQ=60)
                                                    GCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCC                                           <  SRR4302543.2801545/94‑1 (MQ=60)
                                                     CGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCG                                          >  SRR4302543.714702/1‑94 (MQ=60)
                                                      GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                                         >  SRR4302543.6530436/1‑94 (MQ=60)
                                                      GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                                         <  SRR4302543.2451297/94‑1 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGTGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302543.3985504/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302543.7873969/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302543.1059089/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302543.2513014/1‑94 (MQ=60)
                                                        GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       <  SRR4302543.114284/94‑1 (MQ=60)
                                                        GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAGTCTTCGCGTACCGCGCTGGATTCCGTCT                                       <  SRR4302543.6214794/94‑1 (MQ=60)
                                                        GCTTCCCGACGCAGGCGCGTCCCTGCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       <  SRR4302543.114285/94‑1 (MQ=60)
                                                        GCTCCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       <  SRR4302543.6635820/94‑1 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      >  SRR4302543.6714782/1‑94 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      >  SRR4302543.2354846/1‑94 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      <  SRR4302543.7898522/94‑1 (MQ=60)
                                                          TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     >  SRR4302543.4134151/1‑94 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    >  SRR4302543.7277568/1‑94 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302543.4729265/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGCCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302543.218733/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302543.2617364/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   >  SRR4302543.385358/1‑94 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   >  SRR4302543.7925671/1‑94 (MQ=60)
                                                             NCGACGCNGGCGCGTCCCTTNNCNGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  <  SRR4302543.2375596/94‑1 (MQ=60)
                                                             CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  <  SRR4302543.5620096/94‑1 (MQ=60)
                                                              CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 <  SRR4302543.1925312/94‑1 (MQ=60)
                                                              CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 <  SRR4302543.1321988/94‑1 (MQ=60)
                                                              CGACGCAGGCGCGTCCCGTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 <  SRR4302543.4161273/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302543.2142038/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302543.6880569/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302543.6571258/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302543.4675589/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302543.6025940/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302543.7201057/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302543.4675590/94‑1 (MQ=60)
                                                                  GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             <  SRR4302543.227898/94‑1 (MQ=60)
                                                                  GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             <  SRR4302543.6628084/94‑1 (MQ=60)
                                                                   CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            <  SRR4302543.6398076/94‑1 (MQ=60)
                                                                     GGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCG                          <  SRR4302543.5124467/94‑1 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        <  SRR4302543.4667417/94‑1 (MQ=60)
                                                                        GCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTC                       <  SRR4302543.3162691/94‑1 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302543.1512855/1‑94 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302543.732376/1‑94 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302543.5912071/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.327229/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.7291471/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.2270600/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.3607635/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.1398320/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.4468759/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302543.4416864/1‑94 (MQ=60)
                                                                           TCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAT                    <  SRR4302543.7166382/94‑1 (MQ=60)
                                                                           GCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAT                    <  SRR4302543.274274/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  >  SRR4302543.348108/1‑94 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302543.622254/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  >  SRR4302543.475180/1‑94 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  >  SRR4302543.2035723/1‑94 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302543.5535015/94‑1 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 <  SRR4302543.3569732/94‑1 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 >  SRR4302543.4108420/1‑94 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 >  SRR4302543.2137709/1‑94 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 <  SRR4302543.5232179/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302543.4123609/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302543.928123/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302543.1539732/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302543.2257689/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302543.3631840/94‑1 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCTGCGCTCCATATTATC              >  SRR4302543.6739963/1‑94 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATG              <  SRR4302543.2254142/94‑1 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATG              >  SRR4302543.6739964/1‑94 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             >  SRR4302543.4455181/1‑94 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             >  SRR4302543.7960637/1‑94 (MQ=60)
                                                                                   CAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCA            >  SRR4302543.2769111/1‑94 (MQ=60)
                                                                                   CAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCA            >  SRR4302543.6421414/1‑94 (MQ=60)
                                                                                   CAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCA            <  SRR4302543.4180876/94‑1 (MQ=60)
                                                                                    AGCTGGCGCGCGGACGATTACTGTCAGATTCAGCTAATTCTTCGCGTACGGCGCTGGATTTCTTCTCTTTATAGCGGCGCTCCATATTATGCAT           >  SRR4302543.4171003/1‑94 (MQ=46)
                                                                                    AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           <  SRR4302543.3373645/94‑1 (MQ=60)
                                                                                    AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           >  SRR4302543.4171004/1‑94 (MQ=60)
                                                                                     GCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGC          >  SRR4302543.4397805/1‑94 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         <  SRR4302543.5012090/94‑1 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         >  SRR4302543.915359/1‑94 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         <  SRR4302543.893366/94‑1 (MQ=60)
                                                                                       TGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAC        <  SRR4302543.553984/94‑1 (MQ=60)
                                                                                        GGCGCGCGAACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACG       <  SRR4302543.5172934/94‑1 (MQ=60)
                                                                                           GCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCT    <  SRR4302543.3339703/94‑1 (MQ=60)
                                                                                            CGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTT   <  SRR4302543.4537990/94‑1 (MQ=60)
                                                                                             GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  <  SRR4302543.385871/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑