Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,524 T→C H141R (CAC→CGC)  fdhF ← selenopolypeptide subunit of formate dehydrogenase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,5240TC97.6% 739.5 / 3.2 246H141R (CAC→CGC) fdhFselenopolypeptide subunit of formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base C (109/131);  total (112/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 259 total aligned reads displayed.

AACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTA  >  NC_000913/4296434‑4296613
                                                                                          |                                                                                         
aaCACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCgcgt                                                                                         <  1:935835/93‑1 (MQ=255)
 acacTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTc                                                                                        <  1:3609676/93‑1 (MQ=255)
  cacTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCa                                                                                       >  1:1796198/1‑93 (MQ=255)
   acTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAg                                                                                      >  2:501450/1‑93 (MQ=255)
   acTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAg                                                                                      >  1:3644892/1‑93 (MQ=255)
   acTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAg                                                                                      >  2:3818672/1‑93 (MQ=255)
   acTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAg                                                                                      >  1:1628775/1‑93 (MQ=255)
    cTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGa                                                                                     <  2:1934635/93‑1 (MQ=255)
    cTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGa                                                                                     >  2:427105/1‑93 (MQ=255)
     tAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGac                                                                                    <  2:1365456/93‑1 (MQ=255)
     tAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGac                                                                                    >  2:2208486/1‑93 (MQ=255)
     tAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGac                                                                                    >  1:3049783/1‑93 (MQ=255)
      aaaTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTCCCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGaca                                                                                   <  1:1885743/93‑1 (MQ=255)
       aaTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGacac                                                                                  >  2:1778082/1‑93 (MQ=255)
       aaTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGacac                                                                                  <  2:554174/93‑1 (MQ=255)
        aTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACg                                                                                 <  1:1542751/93‑1 (MQ=255)
        aTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACg                                                                                 <  1:4544597/93‑1 (MQ=255)
         tCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGa                                                                                >  2:2473071/1‑93 (MQ=255)
         tCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGAGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACgg                                                                                >  1:2245955/1‑92 (MQ=255)
         acGGTATTATCAATTTCGTTAATAGCATGGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGa                                                                                <  1:3840804/92‑1 (MQ=255)
          cGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAg                                                                               >  2:2977556/1‑93 (MQ=255)
          cGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAg                                                                               <  1:3365772/93‑1 (MQ=255)
           ggTCTTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCTGTGTCAGACACGAgg                                                                              >  1:2830730/1‑92 (MQ=255)
           ggTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgc                                                                              >  2:2337780/1‑93 (MQ=255)
            gTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgcg                                                                             >  2:3990842/1‑93 (MQ=255)
             tattatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgcgc                                                                            >  1:4252481/1‑93 (MQ=255)
             tattatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgcgc                                                                            <  2:4536620/93‑1 (MQ=255)
             tattatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgcgc                                                                            >  1:644274/1‑93 (MQ=255)
             tattatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAgcgc                                                                            <  1:2905081/93‑1 (MQ=255)
               ttatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCgcag                                                                          <  2:1291611/93‑1 (MQ=255)
                tatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCgcagc                                                                         >  2:3863989/1‑93 (MQ=255)
                tatCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCgcagc                                                                         <  2:3364925/93‑1 (MQ=255)
                  tCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGcagcag                                                                       <  1:1749173/93‑1 (MQ=255)
                    aaTTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTc                                                                     <  1:3559437/93‑1 (MQ=255)
                     aTTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCa                                                                    >  1:1606709/1‑93 (MQ=255)
                      tttCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCaa                                                                   >  2:4002383/1‑93 (MQ=255)
                      tttCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCaa                                                                   <  2:3710961/93‑1 (MQ=255)
                          gTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGtt                                                               >  1:1058417/1‑93 (MQ=255)
                           ttAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTa                                                              >  2:2482095/1‑93 (MQ=255)
                           ttAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTa                                                              <  2:202246/93‑1 (MQ=255)
                            tAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTAt                                                             <  1:889192/93‑1 (MQ=255)
                            tAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTAt                                                             <  1:808010/93‑1 (MQ=255)
                             aaTAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTAtt                                                            <  2:2642795/93‑1 (MQ=255)
                               tAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTgg                                                          <  2:806547/93‑1 (MQ=255)
                               tAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTgg                                                          >  1:498377/1‑93 (MQ=255)
                                aGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGt                                                         <  2:428446/93‑1 (MQ=255)
                                 gCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCCGTCAACGTTATTGGTa                                                        >  1:710173/1‑93 (MQ=255)
                                 gCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTa                                                        >  2:4319394/1‑93 (MQ=255)
                                 gCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTa                                                        >  1:1396465/1‑93 (MQ=255)
                                   aTTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTAcc                                                      >  2:2779225/1‑93 (MQ=255)
                                   aTTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTAcc                                                      >  1:441546/1‑93 (MQ=255)
                                   aTTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTAcc                                                      <  1:3183591/93‑1 (MQ=255)
                                      gCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAAt                                                   >  1:4561928/1‑93 (MQ=255)
                                      gCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAAt                                                   <  1:3969620/93‑1 (MQ=255)
                                       cTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATa                                                  >  2:1375105/1‑93 (MQ=255)
                                        tCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATaa                                                 >  1:133310/1‑93 (MQ=255)
                                        tCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATaa                                                 <  1:180689/93‑1 (MQ=255)
                                        tCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATaa                                                 <  2:4109291/93‑1 (MQ=255)
                                        tCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATaa                                                 >  2:4170813/1‑93 (MQ=255)
                                         cATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAAc                                                <  2:722461/93‑1 (MQ=255)
                                          aTTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACg                                               >  1:3503397/1‑93 (MQ=255)
                                          aTTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACg                                               <  1:1852300/93‑1 (MQ=255)
                                           ttGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACgg                                              >  2:1739594/1‑93 (MQ=255)
                                           ttGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACgg                                              >  1:3028877/1‑93 (MQ=255)
                                           ttGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACgg                                              <  2:3270955/93‑1 (MQ=255)
                                            tGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGgc                                             <  1:3992974/93‑1 (MQ=255)
                                              cgcCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGgcgc                                           >  1:3908077/1‑93 (MQ=255)
                                               gcCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACATCATTGGTACCAATAAAGgagcg                                          >  2:2137894/1‑93 (MQ=255)
                                                ccATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGgcgcgc                                         >  2:4447112/1‑93 (MQ=255)
                                                ccATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGgcgcgc                                         <  1:4338805/93‑1 (MQ=255)
                                                  aTTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGgcgcgcgc                                       >  1:4508721/1‑93 (MQ=255)
                                                   ttACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCa                                      >  1:1484742/1‑93 (MQ=255)
                                                   ttACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCa                                      >  2:1084527/1‑93 (MQ=255)
                                                    tACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCaa                                     >  2:2560824/1‑93 (MQ=255)
                                                     accgaccgATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCaaa                                    <  1:1020839/93‑1 (MQ=255)
                                                     accgaccgATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCaaa                                    >  2:600178/1‑93 (MQ=255)
                                                       cgaccgaTTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAAtt                                  <  2:1838303/93‑1 (MQ=255)
                                                        gaccgaTTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAAttt                                 >  1:2366490/1‑93 (MQ=255)
                                                         accgaTTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAAtttt                                >  2:4591651/1‑93 (MQ=255)
                                                            gaTTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGc                             >  2:2684547/1‑93 (MQ=255)
                                                             aTTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCa                            <  1:3819264/93‑1 (MQ=255)
                                                               tGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCAtt                          <  2:4476315/93‑1 (MQ=255)
                                                                ggTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTa                         >  2:4180532/1‑93 (MQ=255)
                                                                 gTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTCCCAATAACGGCGCGCGCAAATTTTTGCATTAc                        <  2:2928145/93‑1 (MQ=255)
                                                                 gTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTAc                        <  1:3115529/93‑1 (MQ=255)
                                                                 gTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTAc                        >  2:3632410/1‑93 (MQ=255)
                                                                   gCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACAt                      <  1:4106508/93‑1 (MQ=255)
                                                                    cAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATa                     <  1:1173609/93‑1 (MQ=255)
                                                                     aGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAg                    <  1:2969038/93‑1 (MQ=255)
                                                                      gACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGt                   >  1:1172998/1‑93 (MQ=255)
                                                                         cTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTgg                <  1:2690529/93‑1 (MQ=255)
                                                                           gCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGtt              <  1:1279690/93‑1 (MQ=255)
                                                                           gCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGtt              <  2:629785/93‑1 (MQ=255)
                                                                             aaCCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTc            >  2:1597240/1‑93 (MQ=255)
                                                                               ccGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGt          <  1:2202163/93‑1 (MQ=255)
                                                                                cGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGtt         <  2:2609255/93‑1 (MQ=255)
                                                                                cGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGtt         <  1:2558073/93‑1 (MQ=255)
                                                                                 gATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTa        <  2:1423444/93‑1 (MQ=255)
                                                                                   tGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTAcc      >  2:3129265/1‑93 (MQ=255)
                                                                                    gggCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTAccc     <  2:2851898/93‑1 (MQ=255)
                                                                                          |                                                                                         
AACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTA  >  NC_000913/4296434‑4296613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCAC  >  NC_000913/4296432‑4296617
                                                                                            |                                                                                             
CGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCG                                                                                              >  SRR4302535.4043444/1‑94 (MQ=60)
CGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCG                                                                                              >  SRR4302535.2253475/1‑94 (MQ=60)
 GAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGT                                                                                             <  SRR4302535.1753639/94‑1 (MQ=60)
  AACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTC                                                                                            <  SRR4302535.942462/94‑1 (MQ=60)
  AACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTC                                                                                            <  SRR4302535.3853326/94‑1 (MQ=60)
   ACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCA                                                                                           <  SRR4302535.92397/94‑1 (MQ=60)
   ACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCA                                                                                           <  SRR4302535.3634712/94‑1 (MQ=60)
   ACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCA                                                                                           <  SRR4302535.1812328/94‑1 (MQ=60)
   ACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCA                                                                                           >  SRR4302535.1809183/1‑94 (MQ=60)
   ACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCCCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCA                                                                                           <  SRR4302535.4491637/94‑1 (MQ=60)
    CACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAG                                                                                          >  SRR4302535.3670135/1‑94 (MQ=60)
    CACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAG                                                                                          >  SRR4302535.1764823/1‑94 (MQ=60)
    CACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAG                                                                                          >  SRR4302535.1640538/1‑94 (MQ=60)
     ACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGA                                                                                         >  SRR4302535.1339345/1‑94 (MQ=60)
      CTAATTCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGAC                                                                                        <  SRR4302535.3067999/94‑1 (MQ=60)
      CTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGAC                                                                                        >  SRR4302535.1888596/1‑94 (MQ=60)
      CTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGAC                                                                                        >  SRR4302535.3071147/1‑94 (MQ=60)
      CTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGAC                                                                                        >  SRR4302535.1839609/1‑94 (MQ=60)
        AAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTCCCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACAC                                                                                      <  SRR4302535.1899329/94‑1 (MQ=60)
        AAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACAC                                                                                      >  SRR4302535.716025/1‑94 (MQ=60)
        AAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACAC                                                                                      <  SRR4302535.3241563/94‑1 (MQ=60)
        AAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACAC                                                                                      <  SRR4302535.1771437/94‑1 (MQ=60)
         AATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACG                                                                                     <  SRR4302535.3643744/94‑1 (MQ=60)
         AATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACG                                                                                     <  SRR4302535.3971276/94‑1 (MQ=60)
         AATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACG                                                                                     <  SRR4302535.4273980/94‑1 (MQ=60)
          ATCGTTATTATCATTTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGCCCTGCAACCGATGGGCCGCGTCAGACACGA                                                                                    <  SRR4302535.4075640/94‑1 (MQ=60)
          ATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGA                                                                                    <  SRR4302535.1553870/94‑1 (MQ=60)
          ATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGA                                                                                    <  SRR4302535.4575678/94‑1 (MQ=60)
          ATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGAGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGG                                                                                    >  SRR4302535.2261925/1‑94 (MQ=60)
           TCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAG                                                                                   <  SRR4302535.1636723/94‑1 (MQ=60)
           TCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAG                                                                                   <  SRR4302535.1707164/94‑1 (MQ=60)
           TCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAG                                                                                   <  SRR4302535.519819/94‑1 (MQ=60)
           ACGGTATTATCAATTTCGTTAATAGCATGGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAG                                                                                   <  SRR4302535.3867270/94‑1 (MQ=60)
            CGGTCTTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCTGTGTCAGACACGAGG                                                                                  >  SRR4302535.2850822/1‑94 (MQ=60)
            CGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGC                                                                                  <  SRR4302535.3389193/94‑1 (MQ=60)
             GGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCG                                                                                 <  SRR4302535.4078101/94‑1 (MQ=60)
              GTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGC                                                                                >  SRR4302535.3482615/1‑94 (MQ=60)
              GTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGC                                                                                >  SRR4302535.4281618/1‑94 (MQ=60)
              GTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGC                                                                                >  SRR4302535.648771/1‑94 (MQ=60)
               TATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCA                                                                               <  SRR4302535.2925616/94‑1 (MQ=60)
                  TATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCA                                                                            <  SRR4302535.4688576/94‑1 (MQ=60)
                    attagttcggtaagtGCATTTCTCATTTCGCCATTACCGACCGATGGGTGCAGCCCTGGAACCGATGGGCCCCGGCAGACACGAGCGCAGCAGT                                                                          <  SRR4302535.1259326/79‑1 (MQ=37)
                    TCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGT                                                                          <  SRR4302535.1761825/94‑1 (MQ=60)
                     CAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTC                                                                         <  SRR4302535.1745352/94‑1 (MQ=60)
                     CAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTC                                                                         <  SRR4302535.4243041/94‑1 (MQ=60)
                     CAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTC                                                                         <  SRR4302535.1409409/94‑1 (MQ=60)
                      AATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCA                                                                        <  SRR4302535.3584121/94‑1 (MQ=60)
                      AATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCA                                                                        >  SRR4302535.1618302/1‑94 (MQ=60)
                       ATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAA                                                                       >  SRR4302535.1180528/1‑94 (MQ=60)
                        TTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAAC                                                                      <  SRR4302535.3858513/94‑1 (MQ=60)
                         TTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACG                                                                     <  SRR4302535.1181749/94‑1 (MQ=60)
                           CGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTT                                                                   >  SRR4302535.3431079/1‑94 (MQ=60)
                           CGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTT                                                                   >  SRR4302535.1065951/1‑94 (MQ=60)
                             TTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTAT                                                                 <  SRR4302535.695752/94‑1 (MQ=60)
                              TAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATT                                                                <  SRR4302535.813670/94‑1 (MQ=60)
                              TAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATT                                                                <  SRR4302535.895494/94‑1 (MQ=60)
                               AATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTG                                                               >  SRR4302535.369855/1‑94 (MQ=60)
                               AATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTG                                                               >  SRR4302535.2242712/1‑94 (MQ=60)
                               AATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTG                                                               <  SRR4302535.1840624/94‑1 (MQ=60)
                                ATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGG                                                              >  SRR4302535.501873/1‑94 (MQ=60)
                                ATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGG                                                              <  SRR4302535.609739/94‑1 (MQ=60)
                                ATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGG                                                              >  SRR4302535.1581877/1‑94 (MQ=60)
                                  AGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCCGTCAACGTTATTGGTA                                                            >  SRR4302535.715158/1‑94 (MQ=60)
                                  AGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA                                                            <  SRR4302535.4020202/94‑1 (MQ=60)
                                  AGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA                                                            >  SRR4302535.4402749/1‑94 (MQ=60)
                                  AGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA                                                            >  SRR4302535.1406438/1‑94 (MQ=60)
                                    CATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACC                                                          >  SRR4302535.444637/1‑94 (MQ=60)
                                     ATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCA                                                         <  SRR4302535.3205584/94‑1 (MQ=60)
                                     ATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCA                                                         >  SRR4302535.4566284/1‑94 (MQ=60)
                                       TGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAAT                                                       <  SRR4302535.1549587/94‑1 (MQ=60)
                                       TGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAAT                                                       >  SRR4302535.4593061/1‑94 (MQ=60)
                                        GCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATA                                                      <  SRR4302535.3996735/94‑1 (MQ=60)
                                        GCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATA                                                      <  SRR4302535.2884949/94‑1 (MQ=60)
                                        GCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATA                                                      >  SRR4302535.4589387/1‑94 (MQ=60)
                                         CTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAA                                                     >  SRR4302535.134244/1‑94 (MQ=60)
                                         CTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAA                                                     >  SRR4302535.3945288/1‑94 (MQ=60)
                                          TCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAAC                                                    <  SRR4302535.827442/94‑1 (MQ=60)
                                          TCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAAC                                                    <  SRR4302535.181944/94‑1 (MQ=60)
                                           CATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACG                                                   >  SRR4302535.773965/1‑94 (MQ=60)
                                           CATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACG                                                   <  SRR4302535.1375909/94‑1 (MQ=60)
                                           CATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACG                                                   >  SRR4302535.3527748/1‑94 (MQ=60)
                                            ATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGG                                                  >  SRR4302535.361240/1‑94 (MQ=60)
                                            ATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGG                                                  >  SRR4302535.3050080/1‑94 (MQ=60)
                                            ATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGG                                                  <  SRR4302535.1865645/94‑1 (MQ=60)
                                              TGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCG                                                <  SRR4302535.4020287/94‑1 (MQ=60)
                                               GCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGC                                               >  SRR4302535.3934847/1‑94 (MQ=60)
                                                CGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCG                                              <  SRR4302535.302693/94‑1 (MQ=60)
                                                  CCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCG                                            >  SRR4302535.1119316/1‑94 (MQ=60)
                                                  CCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCG                                            >  SRR4302535.2790660/1‑94 (MQ=60)
                                                  CCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCG                                            <  SRR4302535.4368474/94‑1 (MQ=60)
                                                   CATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGC                                           >  SRR4302535.4539660/1‑94 (MQ=60)
                                                    ATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCA                                          >  SRR4302535.1495366/1‑94 (MQ=60)
                                                     TTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAA                                         <  SRR4302535.2262958/94‑1 (MQ=60)
                                                      TACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAA                                        >  SRR4302535.3092747/1‑94 (MQ=60)
                                                       ACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAAT                                       <  SRR4302535.1028093/94‑1 (MQ=60)
                                                         CGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTT                                     >  SRR4302535.2383478/1‑94 (MQ=60)
                                                         CGACCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGAGCGCGCAAATTT                                     >  SRR4302535.2033878/1‑94 (MQ=60)
                                                            CCGATTGGTGCAGACCTGCAACCTATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTG                                  <  SRR4302535.4423567/94‑1 (MQ=60)
                                                            CCGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTG                                  <  SRR4302535.3536716/94‑1 (MQ=60)
                                                             CGATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGC                                 >  SRR4302535.3283791/1‑94 (MQ=60)
                                                              GATTGGTGCAGACCTGCAACCGATGGGCTGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCA                                <  SRR4302535.4541226/94‑1 (MQ=60)
                                                               ATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCAT                               <  SRR4302535.3845619/94‑1 (MQ=60)
                                                               ATTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCAT                               <  SRR4302535.3913149/94‑1 (MQ=60)
                                                                TTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATT                              <  SRR4302535.1391704/94‑1 (MQ=60)
                                                                TTGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATT                              <  SRR4302535.2227154/94‑1 (MQ=60)
                                                                 TGGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTA                             <  SRR4302535.1046802/94‑1 (MQ=60)
                                                                  GGTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTAC                            >  SRR4302535.1217186/1‑94 (MQ=60)
                                                                   GTGCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACA                           <  SRR4302535.3137219/94‑1 (MQ=60)
                                                                     GCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATA                         <  SRR4302535.4134627/94‑1 (MQ=60)
                                                                     GCAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATA                         <  SRR4302535.1572532/94‑1 (MQ=60)
                                                                      CAGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAG                        <  SRR4302535.1181986/94‑1 (MQ=60)
                                                                       AGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGT                       >  SRR4302535.220423/1‑94 (MQ=60)
                                                                       AGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGT                       >  SRR4302535.1181374/1‑94 (MQ=60)
                                                                       AGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGT                       <  SRR4302535.936861/94‑1 (MQ=60)
                                                                       AGACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGT                       <  SRR4302535.2989872/94‑1 (MQ=60)
                                                                        GACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATACCGGCGCGCGCAAATTTTTGCATTACATAGTT                      <  SRR4302535.2637383/94‑1 (MQ=60)
                                                                        GACCTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTT                      <  SRR4302535.3720306/94‑1 (MQ=60)
                                                                           CTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGT                   <  SRR4302535.2709759/94‑1 (MQ=60)
                                                                           CTGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGT                   <  SRR4302535.2405417/94‑1 (MQ=60)
                                                                            TGCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTT                  <  SRR4302535.4327452/94‑1 (MQ=60)
                                                                             GCAACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTT                 <  SRR4302535.1288825/94‑1 (MQ=60)
                                                                               AACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCG               <  SRR4302535.3164667/94‑1 (MQ=60)
                                                                               AACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCG               <  SRR4302535.480582/94‑1 (MQ=60)
                                                                                ACCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGT              >  SRR4302535.3015734/1‑94 (MQ=60)
                                                                                 CCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTT             <  SRR4302535.3598918/94‑1 (MQ=60)
                                                                                 CCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTT             <  SRR4302535.2985254/94‑1 (MQ=60)
                                                                                 CCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTT             <  SRR4302535.2954707/94‑1 (MQ=60)
                                                                                 CCGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTT             <  SRR4302535.2217857/94‑1 (MQ=60)
                                                                                  CGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTA            <  SRR4302535.146249/94‑1 (MQ=60)
                                                                                  CGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTA            <  SRR4302535.1208264/94‑1 (MQ=60)
                                                                                  CGATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTA            <  SRR4302535.2576489/94‑1 (MQ=60)
                                                                                   GATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTAC           >  SRR4302535.281052/1‑94 (MQ=60)
                                                                                   GATGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAACAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTAC           <  SRR4302535.3127305/94‑1 (MQ=60)
                                                                                     TGGGCCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCC         <  SRR4302535.2560407/94‑1 (MQ=60)
                                                                                         CCGCGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTAC     >  SRR4302535.282115/1‑94 (MQ=60)
                                                                                            CGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCAC  >  SRR4302535.1749222/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTACCAATAACGGCGCGCGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCAC  >  NC_000913/4296432‑4296617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑