Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,652	A→G	Y513H (TAT→CAT)	yjcS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,652	0	A	G	99.5%	609.9 / NA	196	Y513H (TAT→CAT)	yjcS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (78/117); minor base T (1/0); total (79/117)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 207 total aligned reads displayed.

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

tggtgCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGc                                                                                         <  2:3267405/93‑1 (MQ=255)

 ggtgCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGcc                                                                                        <  1:949326/93‑1 (MQ=255)

     ccGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGc                                                                                    >  2:3231548/1‑93 (MQ=255)

     ccGGGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGc                                                                                    <  2:2890425/93‑1 (MQ=255)

      cGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCt                                                                                   <  1:3815311/93‑1 (MQ=255)

       gTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTg                                                                                  >  1:4677097/1‑93 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  2:649400/93‑1 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  2:1418488/93‑1 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGCAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 >  2:1118068/1‑93 (MQ=255)

         ggCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGtt                                                                                >  1:562154/1‑93 (MQ=255)

         ggCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGtt                                                                                <  1:2222257/93‑1 (MQ=255)

          gCTGAACTTATGCACCCCTTCGCGCTGCTCTTTTGCGCCGTTCTGGTAGAAATCGCGCCATGTGTCGGACTCGGCCTGATGGCCCAGCTGTTc                                                                               >  2:4686887/1‑93 (MQ=255)

          gCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTc                                                                               >  1:3655770/1‑93 (MQ=255)

          gCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTc                                                                               <  1:1564646/93‑1 (MQ=255)

          gCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTc                                                                               >  1:579635/1‑93 (MQ=255)

           cTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCa                                                                              <  2:2135456/93‑1 (MQ=255)

            tGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaa                                                                             >  2:4594862/1‑93 (MQ=255)

            tGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaa                                                                             >  1:1629911/1‑93 (MQ=255)

             gAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaaa                                                                            >  2:4531901/1‑93 (MQ=255)

             gAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaaa                                                                            >  2:267271/1‑93 (MQ=255)

             gAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaaa                                                                            <  2:3905789/93‑1 (MQ=255)

             gAACTTATGCACCCCTTCGCGCAGCTCTTTCACGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaaa                                                                            <  1:368523/93‑1 (MQ=255)

              aaCTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAg                                                                           <  2:2612819/93‑1 (MQ=255)

               aCTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGt                                                                          >  2:4157718/1‑93 (MQ=255)

                cTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGtt                                                                         <  1:1200203/93‑1 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGCCTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        <  2:1818091/93‑1 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        <  1:4025906/93‑1 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        <  1:4180266/93‑1 (MQ=255)

                  tATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAt                                                                       <  1:3205273/93‑1 (MQ=255)

                    tGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTc                                                                     <  2:2346132/93‑1 (MQ=255)

                       aCCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCt                                                                  <  2:1395902/93‑1 (MQ=255)

                        ccccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCtt                                                                 >  2:531830/1‑93 (MQ=255)

                        ccccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCtt                                                                 <  2:1130051/93‑1 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               <  1:4514423/93‑1 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               <  2:3718979/93‑1 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTAGCTTGc                                                               >  1:1744984/1‑93 (MQ=255)

                           cTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCa                                                              <  2:757860/93‑1 (MQ=255)

                           cTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCa                                                              >  2:49630/1‑93 (MQ=255)

                           cTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCa                                                              <  2:2818526/93‑1 (MQ=255)

                            ttCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAg                                                             >  2:3550715/1‑93 (MQ=255)

                              cgcgCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAt                                                           >  2:3640049/1‑93 (MQ=255)

                               gcgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAtt                                                          >  2:1195833/1‑93 (MQ=255)

                               gcgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAtt                                                          <  1:1873517/93‑1 (MQ=255)

                                cgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttc                                                         <  1:3039297/93‑1 (MQ=255)

                                cgcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttc                                                         <  2:202167/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGTCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        <  2:4234486/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        <  1:2848780/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        <  2:3066643/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        >  1:1947870/1‑93 (MQ=255)

                                   aactctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      >  2:2959950/3‑93 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  2:339542/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  1:680017/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  1:3083498/93‑1 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      >  2:4097755/1‑93 (MQ=255)

                                   aGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc                                                      <  2:3991362/93‑1 (MQ=255)

                                    gCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcg                                                     <  1:3466967/93‑1 (MQ=255)

                                    gCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGGGCAAAGTGATTCGCTTGTAGAttcttgg                                                     >  2:2497337/1‑91 (MQ=255)

                                     ctctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgc                                                    >  1:4378078/1‑93 (MQ=255)

                                       ctTTCGCGCCGGTCATGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGTCCAGCTGTTCAAAGTTATTCGCTTTGAGATTCTTCACGa                                                  >  2:4670808/1‑93 (MQ=255)

                                       ctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcgc                                                  >  2:163980/1‑92 (MQ=255)

                                           cgcgCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTg                                              <  2:2213584/93‑1 (MQ=255)

                                           cgcgCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTg                                              <  1:2750568/93‑1 (MQ=255)

                                            gcgcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTgg                                             <  2:1703724/93‑1 (MQ=255)

                                                cGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAc                                         >  1:2587623/1‑93 (MQ=255)

                                                    cAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCgg                                     >  1:763322/1‑93 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  2:1535311/93‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  1:4336043/93‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   >  2:3330133/1‑93 (MQ=255)

                                                       gTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGtt                                  >  1:2480518/1‑93 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             <  1:2013053/93‑1 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             >  1:2122821/1‑93 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             >  2:3435290/1‑93 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             >  1:2805232/1‑93 (MQ=255)

                                                             aCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcggc                            >  2:1908076/1‑93 (MQ=255)

                                                             aCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcggc                            <  1:2811735/93‑1 (MQ=255)

                                                               cgcgCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCaa                          <  1:1810362/93‑1 (MQ=255)

                                                                gcgcCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAAt                         <  1:2171361/93‑1 (MQ=255)

                                                                 cgcCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATc                        <  1:698345/93‑1 (MQ=255)

                                                                 cgcCATGTGCCGGACTCGGCCTGATGCCCAAGCTGTCAAAACTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCGGGGTTGGCGGCAATc                        <  2:2981216/93‑1 (MQ=255)

                                                                  gcCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCa                       <  2:2539017/93‑1 (MQ=255)

                                                                   ccATGTGGCGGACTCGTCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCAc                      <  2:596436/93‑1 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    >  2:2451464/1‑93 (MQ=255)

                                                                          gCGGACTCGGCCTGATGGCCCAGCTGTTCAAATTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTc               <  2:159954/93‑1 (MQ=255)

                                                                            ggACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTACAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcag             <  2:641657/93‑1 (MQ=255)

                                                                               cTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagcag          >  2:3531648/1‑93 (MQ=255)

                                                                               cTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagcag          >  2:2385112/1‑93 (MQ=255)

                                                                               cTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagcag          <  1:4403294/93‑1 (MQ=255)

                                                                                tCGTCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGt         <  2:4422717/93‑1 (MQ=255)

                                                                                tCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGGGACCTCATCACACGGGCTAGCGGCCATCACCTGTTTGAGCAGt         >  1:793618/1‑93 (MQ=255)

                                                                                tCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGt         <  1:3320204/93‑1 (MQ=255)

                                                                                 cGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGtt        >  2:3839801/1‑93 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      <  2:856175/93‑1 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      >  2:3993957/1‑93 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      <  1:3403247/93‑1 (MQ=255)

                                                                                     cTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGc    >  1:3613041/1‑93 (MQ=255)

                                                                                     cTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGc    >  2:4215150/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   <  1:1022406/93‑1 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  1:2357863/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  2:379172/1‑93 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCg  <  2:1585034/93‑1 (MQ=255)

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCA  >  NC_000913/4302559‑4302745

CGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATG                                                                                               <  SRR4302535.1726187/94‑1 (MQ=60)

    GGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCC                                                                                           <  SRR4302535.956049/94‑1 (MQ=60)

       GCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGC                                                                                        >  SRR4302535.4114848/1‑94 (MQ=60)

        CCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCT                                                                                       <  SRR4302535.2721061/94‑1 (MQ=60)

         CGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTG                                                                                      >  SRR4302535.4708663/1‑94 (MQ=60)

         CGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTG                                                                                      <  SRR4302535.3841635/94‑1 (MQ=60)

           TGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTT                                                                                    >  SRR4302535.566084/1‑94 (MQ=60)

           TGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTT                                                                                    <  SRR4302535.445831/94‑1 (MQ=60)

            GGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTC                                                                                   >  SRR4302535.583711/1‑94 (MQ=60)

            GGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTC                                                                                   >  SRR4302535.3681075/1‑94 (MQ=60)

            GGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTC                                                                                   <  SRR4302535.2238074/94‑1 (MQ=60)

             GCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCA                                                                                  <  SRR4302535.1575944/94‑1 (MQ=60)

              CTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAA                                                                                 >  SRR4302535.1641682/1‑94 (MQ=60)

              CTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAA                                                                                 <  SRR4302535.2868358/94‑1 (MQ=60)

               TGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAGTCGGCCTGATGGCCCAGCTGTTCAAA                                                                                >  SRR4302535.389821/1‑94 (MQ=60)

                GAACTTATGCACCCCTTCGCGCAGCTCTTTCACGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAG                                                                               <  SRR4302535.371067/94‑1 (MQ=60)

                  ACTTATCCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTT                                                                             <  SRR4302535.1113869/94‑1 (MQ=60)

                   CTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTA                                                                            <  SRR4302535.1208803/94‑1 (MQ=60)

                    TTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAT                                                                           <  SRR4302535.4208875/94‑1 (MQ=60)

                    TTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTAT                                                                           <  SRR4302535.4053451/94‑1 (MQ=60)

                     TATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATT                                                                          <  SRR4302535.3465172/94‑1 (MQ=60)

                     TATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATT                                                                          <  SRR4302535.3198349/94‑1 (MQ=60)

                     TATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATT                                                                          <  SRR4302535.3227470/94‑1 (MQ=60)

                      ATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTC                                                                         <  SRR4302535.1807607/94‑1 (MQ=60)

                       TGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCG                                                                        <  SRR4302535.1950965/94‑1 (MQ=60)

                         CACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGCCCCATCTGTTCAAAGTTATTCGCT                                                                      <  SRR4302535.4310895/94‑1 (MQ=60)

                          CCCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTT                                                                     <  SRR4302535.2829538/94‑1 (MQ=60)

                          CCCCCCTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTT                                                                     <  SRR4302535.1532451/94‑1 (MQ=60)

                          ACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTT                                                                     <  SRR4302535.787874/94‑1 (MQ=60)

                           TCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTG                                                                    >  SRR4302535.2239888/1‑94 (MQ=60)

                            CCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGC                                                                   >  SRR4302535.363516/1‑94 (MQ=60)

                            CCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTAGCTTGC                                                                   >  SRR4302535.1757611/1‑94 (MQ=60)

                             CCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCA                                                                  >  SRR4302535.2226820/1‑94 (MQ=60)

                             CCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCA                                                                  <  SRR4302535.4545378/94‑1 (MQ=60)

                             CCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCA                                                                  <  SRR4302535.3652701/94‑1 (MQ=60)

                                 CGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATT                                                              >  SRR4302535.4371151/1‑94 (MQ=60)

                                  GCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTC                                                             <  SRR4302535.1887018/94‑1 (MQ=60)

                                   CGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCT                                                            <  SRR4302535.2457215/94‑1 (MQ=60)

                                   CGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCT                                                            >  SRR4302535.1961895/1‑94 (MQ=60)

                                   CGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCT                                                            <  SRR4302535.3060583/94‑1 (MQ=60)

                                    GCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTT                                                           <  SRR4302535.2868967/94‑1 (MQ=60)

                                      AGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGTCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCG                                                         <  SRR4302535.1833496/94‑1 (MQ=60)

                                      AGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCG                                                         <  SRR4302535.684781/94‑1 (MQ=60)

                                      AGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCG                                                         <  SRR4302535.3104985/94‑1 (MQ=60)

                                      AGCTATTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCG                                                         >  SRR4302535.441309/1‑94 (MQ=60)

                                       GCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGC                                                        >  SRR4302535.4408034/1‑94 (MQ=60)

                                       GCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGC                                                        <  SRR4302535.3491059/94‑1 (MQ=60)

                                        CTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG                                                       <  SRR4302535.4653846/94‑1 (MQ=60)

                                        CTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG                                                       >  SRR4302535.476336/1‑94 (MQ=60)

                                         TCTTTCGCGCCGGTCATGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGA                                                      <  SRR4302535.4012178/94‑1 (MQ=60)

                                          CTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAC                                                     >  SRR4302535.744881/1‑94 (MQ=60)

                                            TTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCT                                                   <  SRR4302535.581111/94‑1 (MQ=60)

                                              CGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGG                                                 <  SRR4302535.2770176/94‑1 (MQ=60)

                                                  CCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAC                                             >  SRR4302535.2606204/1‑94 (MQ=60)

                                                      TCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGG                                         <  SRR4302535.2869165/94‑1 (MQ=60)

                                                      TCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGG                                         >  SRR4302535.768698/1‑94 (MQ=60)

                                                        AGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGT                                       >  SRR4302535.1709655/1‑94 (MQ=60)

                                                         GGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTT                                      <  SRR4302535.4365699/94‑1 (MQ=60)

                                                         GGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTT                                      >  SRR4302535.2498404/1‑94 (MQ=60)

                                                            AGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGC                                   <  SRR4302535.1965819/94‑1 (MQ=60)

                                                            AGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGC                                   >  SRR4302535.1592557/1‑94 (MQ=60)

                                                              AAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGG                                 <  SRR4302535.327705/94‑1 (MQ=60)

                                                              AAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGG                                 >  SRR4302535.2825178/1‑94 (MQ=60)

                                                              AAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGG                                 >  SRR4302535.2138123/1‑94 (MQ=60)

                                                               AACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGC                                <  SRR4302535.2027555/94‑1 (MQ=60)

                                                                ACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCA                               <  SRR4302535.2831720/94‑1 (MQ=60)

                                                                  CGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAAT                             <  SRR4302535.1823456/94‑1 (MQ=60)

                                                                  CGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAAT                             >  SRR4302535.2607109/1‑94 (MQ=60)

                                                                   GCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATC                            <  SRR4302535.2186901/94‑1 (MQ=60)

                                                                    CGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCA                           <  SRR4302535.2172621/94‑1 (MQ=60)

                                                                    CGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCA                           <  SRR4302535.703245/94‑1 (MQ=60)

                                                                       CATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCT                        <  SRR4302535.336849/94‑1 (MQ=60)

                                                                       CATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCT                        <  SRR4302535.2114135/94‑1 (MQ=60)

                                                                             GCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCA                  <  SRR4302535.697402/94‑1 (MQ=60)

                                                                               GGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGC                >  SRR4302535.3093217/1‑94 (MQ=60)

                                                                                GACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCA               <  SRR4302535.868253/94‑1 (MQ=60)

                                                                                 ACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAG              <  SRR4302535.638386/94‑1 (MQ=60)

                                                                                  CTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGGGACCTCATCACACGGGCTAGCGGCCATCACCTGTTTGAGCAGT             >  SRR4302535.799194/1‑94 (MQ=53)

                                                                                  CTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGT             <  SRR4302535.4433428/94‑1 (MQ=60)

                                                                                   TCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTT            >  SRR4302535.3709405/1‑94 (MQ=60)

                                                                                   TCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTT            <  SRR4302535.3343306/94‑1 (MQ=60)

                                                                                      GCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTG         <  SRR4302535.3426932/94‑1 (MQ=60)

                                                                                      GCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTG         <  SRR4302535.2780951/94‑1 (MQ=60)

                                                                                       CCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGC        >  SRR4302535.3638094/1‑94 (MQ=60)

                                                                                        CTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCC       >  SRR4302535.2374775/1‑94 (MQ=60)

                                                                                         TGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG      >  SRR4302535.3732367/1‑94 (MQ=60)

                                                                                         TGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG      <  SRR4302535.1029670/94‑1 (MQ=60)

                                                                                          GATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGA     <  SRR4302535.2603752/94‑1 (MQ=60)

                                                                                           ATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGAC    >  SRR4302535.126237/1‑94 (MQ=60)

                                                                                            TGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACC   <  SRR4302535.51634/94‑1 (MQ=60)

                                                                                            TGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACC   >  SRR4302535.2406439/1‑94 (MQ=60)

                                                                                             GGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCA  <  SRR4302535.4615703/94‑1 (MQ=60)

                                                                                             GGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCA  <  SRR4302535.2213548/94‑1 (MQ=60)

CGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCA  >  NC_000913/4302559‑4302745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`