Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,307,603 T→C E319E (GAA→GAG)  yjcW ← putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,6030TC99.4% 1078.5 / ‑6.4 348E319E (GAA→GAG) yjcWputative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base C (178/167);  minor base A (2/0);  total (181/167)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC  >  NC_000913/4307513‑4307692
                                                                                          |                                                                                         
aTCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAtctc                                                                                         >  2:7529317/1‑93 (MQ=255)
aTCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAtctc                                                                                         >  1:374377/1‑93 (MQ=255)
aTCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAtctc                                                                                         <  1:281242/93‑1 (MQ=255)
 tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATctct                                                                                        <  1:6977605/93‑1 (MQ=255)
   ccGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTcc                                                                                      >  2:4232785/1‑93 (MQ=255)
      gcggcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGcc                                                                                   >  1:2290195/1‑93 (MQ=255)
        ggcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAg                                                                                 >  2:6162670/1‑93 (MQ=255)
         gcTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGGTATATTTGCCATTAAGAAGGATCTCTCCGCCAgc                                                                                >  1:430612/1‑93 (MQ=255)
           tttCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAgcgc                                                                              >  2:3165504/1‑93 (MQ=255)
           tttCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAgcgc                                                                              >  1:5596011/1‑93 (MQ=255)
             tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGt                                                                            <  2:1405296/93‑1 (MQ=255)
             tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGt                                                                            >  1:90283/1‑93 (MQ=255)
             tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGt                                                                            >  1:7391974/1‑93 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         >  2:6279104/1‑93 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         <  1:477914/93‑1 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         >  1:2983247/1‑93 (MQ=255)
                gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTa                                                                         <  2:292454/93‑1 (MQ=255)
                 tGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAt                                                                        <  2:2681332/93‑1 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       <  1:2639333/93‑1 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       >  1:7659815/1‑93 (MQ=255)
                  gATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGCGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATc                                                                       >  1:3598017/1‑93 (MQ=255)
                   aTGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATcc                                                                      >  2:4007571/1‑93 (MQ=255)
                    tGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCa                                                                     <  2:7678303/93‑1 (MQ=255)
                    tGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCa                                                                     <  1:5006110/93‑1 (MQ=255)
                    tGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCa                                                                     <  2:3487305/93‑1 (MQ=255)
                    tGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCa                                                                     >  1:1718896/1‑93 (MQ=255)
                        aGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGcc                                                                 <  1:4542846/93‑1 (MQ=255)
                           cATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCaaa                                                              >  1:6583868/1‑93 (MQ=255)
                            aTCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCCTTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCacac                                                             >  2:2203276/1‑93 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            <  1:7788377/93‑1 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            >  2:1989536/1‑93 (MQ=255)
                             tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACa                                                            <  2:6776297/93‑1 (MQ=255)
                                cTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAc                                                         <  2:3933161/93‑1 (MQ=255)
                                cTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAc                                                         <  1:2077107/93‑1 (MQ=255)
                                cTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGAc                                                         <  1:2272901/93‑1 (MQ=255)
                                 ttttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACa                                                        >  2:5152743/1‑93 (MQ=255)
                                  tttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                       <  1:6151715/93‑1 (MQ=255)
                                  tttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACaa                                                       >  1:4673164/1‑93 (MQ=255)
                                      tCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtca                                                   <  2:472004/93‑1 (MQ=255)
                                        aCGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATtcatc                                                 <  1:5340316/93‑1 (MQ=255)
                                              tCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttca                                           <  2:1692191/93‑1 (MQ=255)
                                              tCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttca                                           >  2:6832777/1‑93 (MQ=255)
                                               ccAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcag                                          <  2:5876204/93‑1 (MQ=255)
                                               ccAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcag                                          <  1:7724516/93‑1 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                         <  2:3734936/93‑1 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                         <  1:2010736/93‑1 (MQ=255)
                                                cAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAtcagttcagt                                         >  1:6314298/1‑93 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        <  1:721287/93‑1 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        >  2:2833116/1‑93 (MQ=255)
                                                 aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTa                                        >  2:7412363/1‑93 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       <  2:7133740/93‑1 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       <  2:4309080/93‑1 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       <  2:1600908/93‑1 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       >  1:1088730/1‑93 (MQ=255)
                                                  ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATATCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAc                                       >  2:1182119/1‑93 (MQ=255)
                                                   gggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACg                                      >  2:6630783/1‑93 (MQ=255)
                                                    ggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGt                                     <  1:2486791/93‑1 (MQ=255)
                                                     gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTc                                    <  1:7506706/93‑1 (MQ=255)
                                                     gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTc                                    <  2:5615322/93‑1 (MQ=255)
                                                      tGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTcc                                   >  1:6766918/1‑93 (MQ=255)
                                                      tGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTcc                                   <  2:4494819/93‑1 (MQ=255)
                                                           gTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                              <  2:4198426/93‑1 (MQ=255)
                                                           gTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAc                              <  1:7592470/93‑1 (MQ=255)
                                                            tGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCTTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAcc                             >  2:6344069/1‑93 (MQ=255)
                                                             ggAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCg                            >  1:1496190/1‑93 (MQ=255)
                                                             ggAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGGTTATTCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCg                            >  2:6950034/1‑93 (MQ=255)
                                                              gagagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGCACCGa                           >  2:3078924/1‑93 (MQ=255)
                                                               agagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                          >  2:6001340/1‑93 (MQ=255)
                                                               agagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                          <  1:5766207/93‑1 (MQ=255)
                                                                gagaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAcc                         <  1:5432175/93‑1 (MQ=255)
                                                                 agaTATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCa                        >  1:1414154/1‑93 (MQ=255)
                                                                   atatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCAGAACCGACCAGt                      >  1:1002659/1‑93 (MQ=255)
                                                                    tatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTc                     >  2:5362697/1‑93 (MQ=255)
                                                                    tatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTc                     >  1:3379677/1‑93 (MQ=255)
                                                                    tatCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTc                     <  1:6918643/93‑1 (MQ=255)
                                                                    tatCTTTGCCATGAAGACGGATCTCTCCGCCAGCGAGTTTATCCACGCCAAACAGACAATTCATCAGTCCAGTACGTCCGGAACCGACCAGTc                     <  2:3905439/93‑1 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    <  1:3969784/93‑1 (MQ=255)
                                                                     atCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                    >  1:2497622/1‑93 (MQ=255)
                                                                        tttGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGc                 <  2:4385263/93‑1 (MQ=255)
                                                                         ttGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa                >  1:4170319/1‑93 (MQ=255)
                                                                          tGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCaa               <  1:3677285/93‑1 (MQ=255)
                                                                          tGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCaa               <  2:261031/93‑1 (MQ=255)
                                                                            ccATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg             >  1:5868614/1‑93 (MQ=255)
                                                                             cATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc            <  1:4396740/93‑1 (MQ=255)
                                                                              aTTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc           >  2:1094269/1‑93 (MQ=255)
                                                                              aTTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc           >  2:5451003/1‑93 (MQ=255)
                                                                              aTTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc           >  1:3819799/1‑93 (MQ=255)
                                                                               ttAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCt          <  1:3096591/93‑1 (MQ=255)
                                                                               ttAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCt          >  1:3905395/1‑93 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        <  2:524754/93‑1 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        <  1:6079950/93‑1 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        <  1:1181848/93‑1 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        <  1:7341683/93‑1 (MQ=255)
                                                                                 aaGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTaa        >  2:816795/1‑93 (MQ=255)
                                                                                  aGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAat       >  2:6441672/1‑93 (MQ=255)
                                                                                   gACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAata      <  2:2755740/93‑1 (MQ=255)
                                                                                    aCGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAatat     <  1:1559740/93‑1 (MQ=255)
                                                                                       gATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTtc  >  2:6081360/1‑93 (MQ=255)
                                                                                       gATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTtc  <  2:4922446/93‑1 (MQ=255)
                                                                                       gATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTtc  >  2:2458739/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC  >  NC_000913/4307513‑4307692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  NC_000913/4307510‑4307696
                                                                                             |                                                                                             
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               >  SRR4302543.841427/1‑94 (MQ=60)
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATC                                                                                               <  SRR4302543.4408468/94‑1 (MQ=60)
 TTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCT                                                                                              >  SRR4302543.864974/1‑94 (MQ=60)
 TTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGAGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCT                                                                                              >  SRR4302543.3844048/1‑94 (MQ=60)
  TATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTC                                                                                             <  SRR4302543.980220/94‑1 (MQ=60)
  TATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTC                                                                                             >  SRR4302543.377823/1‑94 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            >  SRR4302543.4482832/1‑94 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            >  SRR4302543.736887/1‑94 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            <  SRR4302543.6634715/94‑1 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            >  SRR4302543.3847439/1‑94 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            <  SRR4302543.283735/94‑1 (MQ=60)
   ATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCT                                                                                            <  SRR4302543.2823530/94‑1 (MQ=60)
    TCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTC                                                                                           <  SRR4302543.7043269/94‑1 (MQ=60)
     CCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCC                                                                                          <  SRR4302543.810074/94‑1 (MQ=60)
      CCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCG                                                                                         <  SRR4302543.2297745/94‑1 (MQ=60)
      CCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCG                                                                                         <  SRR4302543.4528891/94‑1 (MQ=60)
        GGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCC                                                                                       >  SRR4302543.2311099/1‑94 (MQ=60)
        GGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCC                                                                                       >  SRR4302543.6915524/1‑94 (MQ=60)
         GCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCA                                                                                      >  SRR4302543.6680433/1‑94 (MQ=60)
          CGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAG                                                                                     >  SRR4302543.565554/1‑94 (MQ=60)
          CGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAG                                                                                     >  SRR4302543.1070828/1‑94 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGGTATATTTGCCATTAAGAAGGATCTCTCCGCCAGC                                                                                    >  SRR4302543.434647/1‑94 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGC                                                                                    <  SRR4302543.3731344/94‑1 (MQ=60)
           GGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAAATATCTTGGCCATTAAGACGGATCTCTCCGCCAGC                                                                                    <  SRR4302543.3731345/94‑1 (MQ=60)
            GCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCG                                                                                   <  SRR4302543.7182146/94‑1 (MQ=60)
             CTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGC                                                                                  <  SRR4302543.1666117/94‑1 (MQ=60)
             CTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGC                                                                                  >  SRR4302543.5646988/1‑94 (MQ=60)
             CTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGC                                                                                  >  SRR4302543.2518590/1‑94 (MQ=60)
             CTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGC                                                                                  >  SRR4302543.2518591/1‑94 (MQ=60)
              TTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCG                                                                                 >  SRR4302543.7968339/1‑94 (MQ=60)
               TTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGT                                                                                >  SRR4302543.7462977/1‑94 (MQ=60)
               TTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGT                                                                                >  SRR4302543.627298/1‑94 (MQ=60)
               TTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGT                                                                                >  SRR4302543.91127/1‑94 (MQ=60)
               TTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGT                                                                                >  SRR4302543.6458950/1‑94 (MQ=60)
                TCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTT                                                                               >  SRR4302543.111938/1‑94 (MQ=60)
                TCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTT                                                                               <  SRR4302543.5058019/94‑1 (MQ=60)
                 CAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTT                                                                              <  SRR4302543.7456413/94‑1 (MQ=60)
                  AGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTA                                                                             >  SRR4302543.3010336/1‑94 (MQ=60)
                  AGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTA                                                                             >  SRR4302543.1447187/1‑94 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            <  SRR4302543.3100530/94‑1 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            <  SRR4302543.3100529/94‑1 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            <  SRR4302543.482331/94‑1 (MQ=60)
                   GTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTAT                                                                            >  SRR4302543.3994205/1‑94 (MQ=60)
                    TGATGTAAGCCGTCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATC                                                                           >  SRR4302543.1571973/1‑94 (MQ=60)
                    TGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATC                                                                           >  SRR4302543.7732419/1‑94 (MQ=60)
                    TGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGCGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATC                                                                           >  SRR4302543.3629768/1‑94 (MQ=60)
                     GATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCC                                                                          <  SRR4302543.2663237/94‑1 (MQ=60)
                     GATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCC                                                                          <  SRR4302543.5835728/94‑1 (MQ=60)
                      ATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCA                                                                         >  SRR4302543.1734244/1‑94 (MQ=60)
                      ATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCA                                                                         <  SRR4302543.3878405/94‑1 (MQ=60)
                      ATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCA                                                                         <  SRR4302543.7614082/94‑1 (MQ=60)
                      ATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCA                                                                         >  SRR4302543.1506995/1‑94 (MQ=60)
                       TGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCAC                                                                        <  SRR4302543.5051675/94‑1 (MQ=60)
                        GTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACG                                                                       >  SRR4302543.7646314/1‑94 (MQ=60)
                         TAAGCCATCCCTTTTTTCACGGCATCCAGGGGGGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGC                                                                      >  SRR4302543.7107654/1‑94 (MQ=60)
                           AGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCA                                                                    <  SRR4302543.4584213/94‑1 (MQ=60)
                           AGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCA                                                                    <  SRR4302543.2055477/94‑1 (MQ=60)
                             CCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAA                                                                  <  SRR4302543.7817282/94‑1 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTTCGCCAGCGCGTTTATCCACGCCAAAC                                                                 >  SRR4302543.4341445/1‑94 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAca                                                                 >  SRR4302543.2475307/1‑92 (MQ=60)
                              CATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAAC                                                                 <  SRR4302543.7733512/94‑1 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                >  SRR4302543.3224617/1‑94 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                <  SRR4302543.5988554/94‑1 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                <  SRR4302543.422187/94‑1 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                >  SRR4302543.1366087/1‑94 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                <  SRR4302543.2970663/94‑1 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACA                                                                <  SRR4302543.3534139/94‑1 (MQ=60)
                               ATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATATCTACGCCAGCGCGTTTATTCACGCCAAACA                                                                >  SRR4302543.5443987/1‑94 (MQ=60)
                                TCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAG                                                               >  SRR4302543.916373/1‑94 (MQ=60)
                                TCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAG                                                               <  SRR4302543.7861793/94‑1 (MQ=60)
                                TCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAG                                                               <  SRR4302543.480319/94‑1 (MQ=60)
                                 CCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGA                                                              >  SRR4302543.6832070/1‑94 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGTATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            >  SRR4302543.4049168/1‑94 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302543.2095658/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302543.2919203/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302543.2293679/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            <  SRR4302543.4896157/94‑1 (MQ=60)
                                   CTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACA                                                            >  SRR4302543.7022442/1‑94 (MQ=60)
                                    TTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAA                                                           >  SRR4302543.4715765/1‑94 (MQ=60)
                                    TTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAA                                                           <  SRR4302543.6823579/94‑1 (MQ=60)
                                     TTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAAT                                                          <  SRR4302543.6209957/94‑1 (MQ=60)
                                         TCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCAT                                                      <  SRR4302543.6723876/94‑1 (MQ=60)
                                          CACGGCATCCAGGGGTGAGCGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATC                                                     <  SRR4302543.4361798/94‑1 (MQ=60)
                                          CACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATC                                                     <  SRR4302543.6213727/94‑1 (MQ=60)
                                          CACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATC                                                     >  SRR4302543.3176051/1‑94 (MQ=60)
                                           ACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCA                                                    <  SRR4302543.2556936/94‑1 (MQ=60)
                                           ACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCA                                                    <  SRR4302543.374001/94‑1 (MQ=60)
                                           ACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCA                                                    <  SRR4302543.5388627/94‑1 (MQ=60)
                                           ACGGCATCCAGGGGTGAACGTGGAGAGATATCTATGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCA                                                    <  SRR4302543.7683435/94‑1 (MQ=60)
                                            CGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAG                                                   >  SRR4302543.171412/1‑94 (MQ=60)
                                            CGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAG                                                   >  SRR4302543.5575138/1‑94 (MQ=60)
                                              GCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTT                                                 <  SRR4302543.3918348/94‑1 (MQ=60)
                                               CATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTC                                                <  SRR4302543.2870517/94‑1 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               <  SRR4302543.3854465/94‑1 (MQ=60)
                                                ATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCA                                               >  SRR4302543.2142966/1‑94 (MQ=60)
                                                 TCCGGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              >  SRR4302543.1521634/1‑94 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              <  SRR4302543.5308873/94‑1 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              <  SRR4302543.7254085/94‑1 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              <  SRR4302543.6917856/94‑1 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              <  SRR4302543.7724715/94‑1 (MQ=60)
                                                 TCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAG                                              >  SRR4302543.1583987/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             <  SRR4302543.7529441/94‑1 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             <  SRR4302543.2048130/94‑1 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             <  SRR4302543.7797544/94‑1 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             >  SRR4302543.6373688/1‑94 (MQ=60)
                                                  CCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGT                                             >  SRR4302543.7671175/1‑94 (MQ=60)
                                                   CAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTA                                            >  SRR4302543.3992089/1‑94 (MQ=60)
                                                   CAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTA                                            >  SRR4302543.4378051/1‑94 (MQ=60)
                                                   CAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTA                                            <  SRR4302543.2028802/94‑1 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           >  SRR4302543.1098514/1‑94 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           <  SRR4302543.727797/94‑1 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           >  SRR4302543.2260937/1‑94 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           >  SRR4302543.6099436/1‑94 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           >  SRR4302543.6613259/1‑94 (MQ=60)
                                                    AGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTAC                                           >  SRR4302543.3401142/1‑94 (MQ=60)
                                                     GGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACG                                          <  SRR4302543.230988/94‑1 (MQ=60)
                                                      GGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGT                                         >  SRR4302543.5713409/1‑94 (MQ=60)
                                                       GGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTC                                        <  SRR4302543.2509318/94‑1 (MQ=60)
                                                        GTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCC                                       >  SRR4302543.195957/1‑94 (MQ=60)
                                                        GTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCC                                       <  SRR4302543.5730016/94‑1 (MQ=60)
                                                        GTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCC                                       >  SRR4302543.5015853/1‑94 (MQ=60)
                                                        GTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCC                                       <  SRR4302543.7578451/94‑1 (MQ=60)
                                                        GTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCC                                       >  SRR4302543.6830825/1‑94 (MQ=60)
                                                           AACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGA                                    <  SRR4302543.618396/94‑1 (MQ=60)
                                                            ACGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAA                                   <  SRR4302543.106525/94‑1 (MQ=60)
                                                             CGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAC                                  >  SRR4302543.2587194/1‑94 (MQ=60)
                                                             CGTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAC                                  >  SRR4302543.554180/1‑94 (MQ=60)
                                                              GTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACC                                 <  SRR4302543.7664605/94‑1 (MQ=60)
                                                              GTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACC                                 <  SRR4302543.4346233/94‑1 (MQ=60)
                                                              GTGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACC                                 <  SRR4302543.1522030/94‑1 (MQ=60)
                                                               TGGAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCG                                >  SRR4302543.1509653/1‑94 (MQ=60)
                                                                 GAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAC                              >  SRR4302543.774330/1‑94 (MQ=60)
                                                                 GAGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAC                              <  SRR4302543.3990432/94‑1 (MQ=60)
                                                                  AGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATTAGTTCAGTACGTCCGGAACCGTCC                             >  SRR4302543.3468008/1‑94 (MQ=60)
                                                                  AGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACC                             >  SRR4302543.4663720/1‑94 (MQ=60)
                                                                  AGAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACC                             <  SRR4302543.5818725/94‑1 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            >  SRR4302543.1426953/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            >  SRR4302543.7394048/1‑94 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            <  SRR4302543.4115625/94‑1 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            <  SRR4302543.5481530/94‑1 (MQ=60)
                                                                   GAGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCA                            <  SRR4302543.1412331/94‑1 (MQ=60)
                                                                    AGATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAG                           >  SRR4302543.7991072/1‑94 (MQ=60)
                                                                     GATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGT                          >  SRR4302543.2029681/1‑94 (MQ=60)
                                                                     GATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGT                          <  SRR4302543.582793/94‑1 (MQ=60)
                                                                     GATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCAGAACCGACCAGT                          >  SRR4302543.1011521/1‑94 (MQ=60)
                                                                      ATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTC                         >  SRR4302543.3410003/1‑94 (MQ=60)
                                                                      ATATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTC                         >  SRR4302543.2320427/1‑94 (MQ=60)
                                                                      ATATCTTTGCCATTAAGACGGATCTCTCCGCCACCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTC                         >  SRR4302543.4069345/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCC                        >  SRR4302543.2520281/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCC                        >  SRR4302543.303172/1‑94 (MQ=60)
                                                                       TATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCC                        <  SRR4302543.6983686/94‑1 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       <  SRR4302543.4004973/94‑1 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       >  SRR4302543.125936/1‑94 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       <  SRR4302543.3677631/94‑1 (MQ=60)
                                                                        ATCTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCG                       <  SRR4302543.5543245/94‑1 (MQ=60)
                                                                          CTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGC                     >  SRR4302543.4767435/1‑94 (MQ=60)
                                                                          CTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGC                     >  SRR4302543.1162906/1‑94 (MQ=60)
                                                                          CTTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGC                     >  SRR4302543.1162905/1‑94 (MQ=60)
                                                                           TTTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCA                    >  SRR4302543.4208080/1‑94 (MQ=60)
                                                                            TTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAA                   <  SRR4302543.3787721/94‑1 (MQ=60)
                                                                            TTGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAA                   >  SRR4302543.7363691/1‑94 (MQ=60)
                                                                             TGCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAA                  <  SRR4302543.3709579/94‑1 (MQ=60)
                                                                              GCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAG                 >  SRR4302543.5922047/1‑94 (MQ=60)
                                                                              GCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAG                 <  SRR4302543.5449220/94‑1 (MQ=60)
                                                                              GCCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAG                 <  SRR4302543.5179603/94‑1 (MQ=60)
                                                                               CCATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGC                >  SRR4302543.2562230/1‑94 (MQ=60)
                                                                                CATTAAGACGGATCTCTCCGCCAGCGCGTTTTTCCACGCCAAACAGACAATTCATAAGTTCAGTACTTCCGGAACCGACCTGTCCGGCAAAGCC               >  SRR4302543.2224740/1‑94 (MQ=60)
                                                                                CATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCC               >  SRR4302543.3853483/1‑94 (MQ=60)
                                                                                 ATTAGGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              >  SRR4302543.4816889/1‑94 (MQ=60)
                                                                                 ATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              >  SRR4302543.3939873/1‑94 (MQ=60)
                                                                                 ATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              >  SRR4302543.7835190/1‑94 (MQ=60)
                                                                                 ATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              >  SRR4302543.3607073/1‑94 (MQ=60)
                                                                                 ATTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCT              >  SRR4302543.493211/1‑94 (MQ=60)
                                                                                  TTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTA             <  SRR4302543.3124444/94‑1 (MQ=60)
                                                                                  TTAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTA             >  SRR4302543.7469308/1‑94 (MQ=60)
                                                                                   TAAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAA            >  SRR4302543.7008115/1‑94 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302543.6137567/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           >  SRR4302543.3664677/1‑94 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           >  SRR4302543.5973753/1‑94 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302543.1192532/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302543.7412314/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           <  SRR4302543.7412313/94‑1 (MQ=60)
                                                                                    AAGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAAT           >  SRR4302543.4551104/1‑94 (MQ=60)
                                                                                     AGACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATA          >  SRR4302543.7609906/1‑94 (MQ=60)
                                                                                      GACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAT         <  SRR4302543.1800678/94‑1 (MQ=60)
                                                                                      GACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAT         <  SRR4302543.7828078/94‑1 (MQ=60)
                                                                                       ACGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATT        <  SRR4302543.1573674/94‑1 (MQ=60)
                                                                                        CGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTT       <  SRR4302543.6190201/94‑1 (MQ=60)
                                                                                        CGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTT       <  SRR4302543.6190200/94‑1 (MQ=60)
                                                                                        CGGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTT       <  SRR4302543.2513362/94‑1 (MQ=60)
                                                                                         GGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC      >  SRR4302543.7478470/1‑94 (MQ=60)
                                                                                         GGATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTC      >  SRR4302543.2305804/1‑94 (MQ=60)
                                                                                          GATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT     <  SRR4302543.3663545/94‑1 (MQ=60)
                                                                                          GATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT     >  SRR4302543.3676265/1‑94 (MQ=60)
                                                                                           ATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCACAGCCTAATATTTCTC    >  SRR4302543.4609256/1‑94 (MQ=60)
                                                                                           ATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTC    <  SRR4302543.6157420/94‑1 (MQ=60)
                                                                                           ATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTC    >  SRR4302543.4609257/1‑94 (MQ=60)
                                                                                           ATCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTC    >  SRR4302543.1049604/1‑94 (MQ=60)
                                                                                            TCTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCACTCCGGCAAAGCCTAATATTTCTCC   <  SRR4302543.7219004/94‑1 (MQ=60)
                                                                                             CTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  SRR4302543.5054104/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCC  >  NC_000913/4307510‑4307696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑