Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,316,203 T→A Q47L (CAG→CTG)  phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2030TA99.6% 798.8 / NA 223Q47L (CAG→CTG) phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (107/115);  minor base C (1/0);  total (108/115)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292
                                                                                         |                                                                                         
ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        <  1:2388858/93‑1 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       <  2:2106832/93‑1 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       <  1:4457726/93‑1 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGGTCGACTTCGGCAGCGTg                                                                                      >  1:1997768/1‑93 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      <  1:2766617/93‑1 (MQ=255)
    ggCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGAGGt                                                                                    >  2:2818583/1‑93 (MQ=255)
     gCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTc                                                                                   <  2:2750890/93‑1 (MQ=255)
      ccTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCt                                                                                  >  2:1519090/1‑93 (MQ=255)
      ccTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCt                                                                                  >  1:1314194/1‑93 (MQ=255)
       cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCtt                                                                                 >  1:3490331/1‑93 (MQ=255)
       cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCtt                                                                                 <  2:3413977/93‑1 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  2:205141/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  1:594654/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  2:220068/1‑93 (MQ=255)
         cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               <  2:74242/93‑1 (MQ=255)
         cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               <  1:2494371/93‑1 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              >  2:4255158/1‑93 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              >  2:2879085/1‑93 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              >  1:3270566/1‑93 (MQ=255)
          ccTCATTGCATACAGCGAACTGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              <  2:1374318/92‑1 (MQ=255)
           cTCATTACATACAGCGAACGTTTCTCGTAGTAAATTCCCCCCTGCGGCGGCGTCGGGCGCGGGGAGATCGACTTCAGCAGCGTGGTCTCCCCg                                                                             <  2:2399519/93‑1 (MQ=255)
            tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg                                                                            >  2:356973/1‑93 (MQ=255)
             cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa                                                                           >  1:4313853/1‑93 (MQ=255)
             cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa                                                                           <  2:3750359/93‑1 (MQ=255)
             cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGGCTTCCCGGa                                                                           >  2:1921406/1‑93 (MQ=255)
             cATTGCACACAGCGAACGGTTCTCGTAGTGAATTGCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGa                                                                           <  2:4386814/93‑1 (MQ=255)
              aTTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                          >  1:3505116/1‑93 (MQ=255)
               ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  1:4615420/1‑93 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  1:4197502/1‑93 (MQ=255)
                      cAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTc                                                                  <  2:3906361/93‑1 (MQ=255)
                       aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc                                                                 >  1:1770171/1‑93 (MQ=255)
                       aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc                                                                 <  2:4408840/93‑1 (MQ=255)
                       aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc                                                                 >  1:3751337/1‑93 (MQ=255)
                        gCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTccc                                                                >  1:2039803/1‑93 (MQ=255)
                         cGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  2:2716108/93‑1 (MQ=255)
                            aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                            >  1:2260023/1‑93 (MQ=255)
                              ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  1:2531852/93‑1 (MQ=255)
                              ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  2:2495238/93‑1 (MQ=255)
                              ggTTCTCGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  2:3871408/93‑1 (MQ=255)
                               gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                         >  1:3361754/1‑93 (MQ=255)
                                ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                        >  2:2655707/1‑93 (MQ=255)
                                   tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     >  1:164640/1‑93 (MQ=255)
                                   tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     >  1:830357/1‑93 (MQ=255)
                                   tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     >  2:1812919/1‑93 (MQ=255)
                                     gTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   <  1:995294/93‑1 (MQ=255)
                                      tAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGc                                                  >  1:3144114/1‑93 (MQ=255)
                                        gTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                <  1:4079345/93‑1 (MQ=255)
                                        gTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                <  2:2677362/93‑1 (MQ=255)
                                         tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt                                               <  1:3574425/93‑1 (MQ=255)
                                         tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt                                               <  2:3806271/93‑1 (MQ=255)
                                          gAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt                                              <  1:2733835/93‑1 (MQ=255)
                                           aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  1:7062/93‑1 (MQ=255)
                                           aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  2:1350676/93‑1 (MQ=255)
                                           aaTGTCCCCCTGCTGCGGCGTCAGGCGCGCGTAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCTACAATGCCCAGCACTTc                                             <  2:4073021/93‑1 (MQ=255)
                                            aTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            >  1:2278816/1‑93 (MQ=255)
                                            aTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            <  2:4494906/93‑1 (MQ=255)
                                                  cccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCCGc                                      >  1:921674/1‑93 (MQ=255)
                                                   ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     >  2:834155/1‑93 (MQ=255)
                                                    ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  2:2344020/1‑93 (MQ=255)
                                                    ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:2851509/1‑93 (MQ=255)
                                                    ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:4419166/1‑93 (MQ=255)
                                                     tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt                                   >  1:2181331/1‑93 (MQ=255)
                                                        tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                >  1:1722664/1‑93 (MQ=255)
                                                        tgcGGCGTCAGGCGCGCGGAGATCGACTACAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                <  2:4168238/93‑1 (MQ=255)
                                                         gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                               <  2:4467446/93‑1 (MQ=255)
                                                          cggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATc                              >  1:1360102/1‑93 (MQ=255)
                                                           ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             <  2:1620405/93‑1 (MQ=255)
                                                           ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             <  2:1702888/93‑1 (MQ=255)
                                                            gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaa                            >  1:1274285/1‑93 (MQ=255)
                                                              gTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTCCCCGGAGCCGGATGCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          <  2:3619235/93‑1 (MQ=255)
                                                               tCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                         >  1:2666530/1‑93 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:107536/93‑1 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        >  1:1594034/1‑93 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        >  2:1313625/1‑93 (MQ=255)
                                                                cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  1:3657957/93‑1 (MQ=255)
                                                                 aGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag                       <  1:2214911/93‑1 (MQ=255)
                                                                  ggggcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:1033305/90‑1 (MQ=255)
                                                                  ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  2:3708534/93‑1 (MQ=255)
                                                                   gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc                     <  1:4662625/93‑1 (MQ=255)
                                                                    gacgcgGAGATCGCCATCAGCAGCGTGGTCTTCACGGAGCCGGATTCCCCGACAAGGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  2:3316666/91‑1 (MQ=255)
                                                                    cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  2:1254084/93‑1 (MQ=255)
                                                                     gcgcgGAGATCGACTTCGGCAGCGTGGTCTTCCCTGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  1:4169196/93‑1 (MQ=255)
                                                                        cgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                <  1:629925/93‑1 (MQ=255)
                                                                         ggAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCt               <  2:2241666/93‑1 (MQ=255)
                                                                         ggAGATCGACTTCAGCAGCGTGGTCTGCCCAGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCt               <  2:3091205/93‑1 (MQ=255)
                                                                           agaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaa             <  1:2607000/93‑1 (MQ=255)
                                                                            gaTCGACTTCAGCAGCGTGGCCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            <  1:339557/93‑1 (MQ=255)
                                                                             aTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAg           >  1:1394222/1‑93 (MQ=255)
                                                                                gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:599509/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGTTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  1:3001557/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  1:3951903/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  1:3824879/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  2:499474/93‑1 (MQ=255)
                                                                                 aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       >  2:566304/1‑93 (MQ=255)
                                                                                  cTTCAGCAGCGTGTTGCTCCCGGAGCCCGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      <  2:350837/93‑1 (MQ=255)
                                                                                  cTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      >  1:1696649/1‑93 (MQ=255)
                                                                                  cTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      >  2:1999605/1‑93 (MQ=255)
                                                                                  cTTCAGCAGCGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      <  1:2866122/93‑1 (MQ=255)
                                                                                      agcagcGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  1:1818787/93‑1 (MQ=255)
                                                                                      agcagcGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  >  2:4654096/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCG  >  NC_000913/4316111‑4316296
                                                                                            |                                                                                             
GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                              <  SRR4302535.428299/94‑1 (MQ=60)
GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                              <  SRR4302535.1367075/94‑1 (MQ=60)
 GCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGC                                                                                             <  SRR4302535.252694/94‑1 (MQ=60)
   GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                           >  SRR4302535.600579/1‑94 (MQ=60)
   GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                           <  SRR4302535.2406030/94‑1 (MQ=60)
    GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGGTCGACTTCGGCAGCGTG                                                                                          >  SRR4302535.2012155/1‑94 (MQ=60)
    GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                          <  SRR4302535.3793641/94‑1 (MQ=60)
    GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                          <  SRR4302535.4488298/94‑1 (MQ=60)
     TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                         <  SRR4302535.2786320/94‑1 (MQ=60)
      CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGT                                                                                        >  SRR4302535.4396836/1‑94 (MQ=60)
        GCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCT                                                                                      >  SRR4302535.1323587/1‑94 (MQ=60)
         CCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTT                                                                                     >  SRR4302535.3514586/1‑94 (MQ=60)
          CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCCGCCTGGTCTTC                                                                                    >  SRR4302535.2362193/1‑94 (MQ=60)
          CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                    >  SRR4302535.598822/1‑94 (MQ=60)
          CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                    >  SRR4302535.116612/1‑94 (MQ=60)
           TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                   >  SRR4302535.490650/1‑94 (MQ=60)
            CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCC                                                                                  <  SRR4302535.2512369/94‑1 (MQ=60)
            CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCC                                                                                  >  SRR4302535.3293284/1‑94 (MQ=60)
              CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGAGTTCAGCAGCGCGGTCTTCCCGG                                                                                >  SRR4302535.1729323/1‑94 (MQ=60)
               TCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGA                                                                               >  SRR4302535.4343361/1‑94 (MQ=60)
                CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAG                                                                              >  SRR4302535.948991/1‑94 (MQ=60)
                CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAG                                                                              >  SRR4302535.3392738/1‑94 (MQ=60)
                CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAG                                                                              >  SRR4302535.3529474/1‑94 (MQ=60)
                 ATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGC                                                                             >  SRR4302535.4646784/1‑94 (MQ=60)
                  TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCC                                                                            <  SRR4302535.3641338/94‑1 (MQ=60)
                  TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCC                                                                            <  SRR4302535.1658744/94‑1 (MQ=60)
                   TGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCG                                                                           >  SRR4302535.2639346/1‑94 (MQ=60)
                    GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                          <  SRR4302535.2722116/94‑1 (MQ=60)
                     CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGA                                                                         >  SRR4302535.4226225/1‑94 (MQ=60)
                      ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                        >  SRR4302535.2948271/1‑94 (MQ=60)
                       TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                       >  SRR4302535.551116/1‑94 (MQ=60)
                         CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCC                                                                     >  SRR4302535.3777303/1‑94 (MQ=60)
                         CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCC                                                                     >  SRR4302535.1783001/1‑94 (MQ=60)
                         CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCC                                                                     <  SRR4302535.2213168/94‑1 (MQ=60)
                          AGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCC                                                                    <  SRR4302535.1351613/94‑1 (MQ=60)
                          AGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCC                                                                    >  SRR4302535.2054493/1‑94 (MQ=60)
                           GCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCC                                                                   <  SRR4302535.3150307/94‑1 (MQ=60)
                            CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                  >  SRR4302535.2752433/1‑94 (MQ=60)
                            CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                  >  SRR4302535.253150/1‑94 (MQ=60)
                            CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                  <  SRR4302535.3541659/94‑1 (MQ=60)
                              AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                                >  SRR4302535.2276115/1‑94 (MQ=60)
                              AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                                >  SRR4302535.1493830/1‑94 (MQ=60)
                                CGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAA                                                              >  SRR4302535.1284852/1‑94 (MQ=60)
                                 GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAT                                                             <  SRR4302535.2550095/94‑1 (MQ=60)
                                 GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAT                                                             >  SRR4302535.2283374/1‑94 (MQ=60)
                                 GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAT                                                             >  SRR4302535.3385149/1‑94 (MQ=60)
                                  GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAgt                                                            <  SRR4302535.429322/94‑3 (MQ=60)
                                  GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                            >  SRR4302535.3347092/1‑94 (MQ=60)
                                    TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                          <  SRR4302535.3095523/94‑1 (MQ=60)
                                    TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                          <  SRR4302535.1403920/94‑1 (MQ=60)
                                     CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                         >  SRR4302535.165791/1‑94 (MQ=60)
                                     CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                         >  SRR4302535.836174/1‑94 (MQ=60)
                                       CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                       >  SRR4302535.4456897/1‑94 (MQ=60)
                                       CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                       >  SRR4302535.3910810/1‑94 (MQ=60)
                                       CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                       <  SRR4302535.2081933/94‑1 (MQ=60)
                                        GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                      >  SRR4302535.3165983/1‑94 (MQ=60)
                                        GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                      <  SRR4302535.1002362/94‑1 (MQ=60)
                                           GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                   <  SRR4302535.4500722/94‑1 (MQ=60)
                                           GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                   >  SRR4302535.1965104/1‑94 (MQ=60)
                                           GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                   <  SRR4302535.3702492/94‑1 (MQ=60)
                                           GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                   <  SRR4302535.4107251/94‑1 (MQ=60)
                                            TGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTT                                                  <  SRR4302535.1792498/94‑1 (MQ=60)
                                            TGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTT                                                  <  SRR4302535.3599207/94‑1 (MQ=60)
                                             GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                 >  SRR4302535.1845689/1‑94 (MQ=60)
                                             GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                 <  SRR4302535.2753344/94‑1 (MQ=60)
                                             GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                 <  SRR4302535.658651/94‑1 (MQ=60)
                                              AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                                <  SRR4302535.7119/94‑1 (MQ=60)
                                              AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                                >  SRR4302535.2295093/1‑94 (MQ=60)
                                               ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTGCCC                                               >  SRR4302535.1691069/1‑94 (MQ=60)
                                                 TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                             <  SRR4302535.2760723/94‑1 (MQ=60)
                                                    CCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCCGC                                          >  SRR4302535.928208/1‑94 (MQ=60)
                                                      CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCC                                        >  SRR4302535.3186601/1‑94 (MQ=60)
                                                      CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                        >  SRR4302535.2871715/1‑94 (MQ=60)
                                                      CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                        >  SRR4302535.4449426/1‑94 (MQ=60)
                                                       CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                       >  SRR4302535.4517544/1‑94 (MQ=60)
                                                       CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                       >  SRR4302535.2599703/1‑94 (MQ=60)
                                                       CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                       >  SRR4302535.2196892/1‑94 (MQ=60)
                                                       CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                       >  SRR4302535.1743696/1‑94 (MQ=60)
                                                        TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                      >  SRR4302535.2480072/1‑94 (MQ=60)
                                                          CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                    >  SRR4302535.1735124/1‑94 (MQ=60)
                                                           TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                   <  SRR4302535.2416702/94‑1 (MQ=60)
                                                           TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                   <  SRR4302535.1324879/94‑1 (MQ=60)
                                                           TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                   <  SRR4302535.3483576/94‑1 (MQ=60)
                                                            GCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                  >  SRR4302535.1369829/1‑94 (MQ=60)
                                                            GCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                  <  SRR4302535.4005582/94‑1 (MQ=60)
                                                              GGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAA                                >  SRR4302535.1283381/1‑94 (MQ=60)
                                                               GCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAA                               <  SRR4302535.2033206/94‑1 (MQ=60)
                                                                CGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                              >  SRR4302535.3729062/1‑94 (MQ=60)
                                                                 GTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                             <  SRR4302535.691049/94‑1 (MQ=60)
                                                                 GTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                             >  SRR4302535.2685618/1‑94 (MQ=60)
                                                                 GTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGACTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                             >  SRR4302535.883334/1‑94 (MQ=60)
                                                                  TCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGA                            >  SRR4302535.1605536/1‑94 (MQ=60)
                                                                   CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                           <  SRR4302535.3683277/94‑1 (MQ=60)
                                                                   CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                           <  SRR4302535.3444755/94‑1 (MQ=60)
                                                                   CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCACCGGCCATAAATCAAAAGAG                           <  SRR4302535.2213068/94‑1 (MQ=60)
                                                                    AGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGA                          <  SRR4302535.2230684/94‑1 (MQ=60)
                                                                     GGGGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                         <  SRR4302535.1040646/94‑1 (MQ=60)
                                                                     GGCGCGCGGAGATCGCCTTAAGCAGCGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                         <  SRR4302535.4109415/94‑1 (MQ=60)
                                                                     GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                         <  SRR4302535.17768/94‑1 (MQ=60)
                                                                      GCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA                        <  SRR4302535.4694156/94‑1 (MQ=60)
                                                                       CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                       <  SRR4302535.4201272/94‑1 (MQ=60)
                                                                       CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                       <  SRR4302535.4212150/94‑1 (MQ=60)
                                                                       CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                       <  SRR4302535.3551952/94‑1 (MQ=60)
                                                                       CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                       <  SRR4302535.3346377/94‑1 (MQ=60)
                                                                       CGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                       >  SRR4302535.2294607/1‑94 (MQ=60)
                                                                        GCGCGGAGATCGACTTCGGCAGCGTGGTCTTCCCTGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATC                      <  SRR4302535.4197747/94‑1 (MQ=60)
                                                                        GCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGATACATC                      <  SRR4302535.734716/94‑1 (MQ=60)
                                                                        GCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATC                      <  SRR4302535.284296/94‑1 (MQ=60)
                                                                         CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                     <  SRR4302535.4650748/94‑1 (MQ=60)
                                                                         CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                     >  SRR4302535.2594024/1‑94 (MQ=60)
                                                                           CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                   >  SRR4302535.3886660/1‑94 (MQ=60)
                                                                           CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                   <  SRR4302535.634317/94‑1 (MQ=60)
                                                                           CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                   <  SRR4302535.2881155/94‑1 (MQ=60)
                                                                              AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA                <  SRR4302535.2625708/94‑1 (MQ=60)
                                                                               GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATCAATCAAAAGAGACATCGCTAAAG               >  SRR4302535.1547202/1‑94 (MQ=60)
                                                                               GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAG               >  SRR4302535.1404179/1‑94 (MQ=60)
                                                                               GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAG               >  SRR4302535.165197/1‑94 (MQ=60)
                                                                               GATCGACTTCAGCAGCGTGGCCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAG               <  SRR4302535.341907/94‑1 (MQ=60)
                                                                                 TCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCC             <  SRR4302535.352926/94‑1 (MQ=60)
                                                                                    ACTTCAGCAGCGTGTTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT          <  SRR4302535.3022597/94‑1 (MQ=60)
                                                                                    ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT          <  SRR4302535.3978885/94‑1 (MQ=60)
                                                                                    ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT          <  SRR4302535.3851261/94‑1 (MQ=60)
                                                                                    ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT          >  SRR4302535.1708913/1‑94 (MQ=60)
                                                                                     CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG         >  SRR4302535.2795902/1‑94 (MQ=60)
                                                                                     CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG         <  SRR4302535.2251839/94‑1 (MQ=60)
                                                                                     CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG         <  SRR4302535.2886423/94‑1 (MQ=60)
                                                                                        CAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC      <  SRR4302535.857382/94‑1 (MQ=60)
                                                                                         AGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG     <  SRR4302535.1831932/94‑1 (MQ=60)
                                                                                         AGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG     >  SRR4302535.1194461/1‑94 (MQ=60)
                                                                                         AGCAGCGTGGTCTTCCCGGAGCCGGATACCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG     <  SRR4302535.4398401/94‑1 (MQ=60)
                                                                                          GCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGG    >  SRR4302535.133252/1‑94 (MQ=60)
                                                                                            AGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGTCCGGCG  >  SRR4302535.4502255/1‑94 (MQ=60)
                                                                                            AGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGAG  >  SRR4302535.968048/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGCG  >  NC_000913/4316111‑4316296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑