Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,343 T→C T249A (ACC→GCC)  b4161 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,3430TC100.0% 593.2 / NA 190T249A (ACC→GCC) b4161hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (87/103);  total (87/103)

Only 100 of 202 total aligned reads displayed.

CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC  >  NC_000913/4388253‑4388432
                                                                                          |                                                                                         
ccAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGgtgt                                                                                         <  2:3378817/93‑1 (MQ=255)
ccAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGgcgt                                                                                         <  1:1604183/93‑1 (MQ=255)
  aGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGc                                                                                       <  1:2445882/93‑1 (MQ=255)
   ggTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCt                                                                                      <  2:3451076/93‑1 (MQ=255)
    gTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTg                                                                                     >  1:2189199/1‑93 (MQ=255)
      gCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAc                                                                                   <  2:823382/93‑1 (MQ=255)
      gCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAc                                                                                   >  1:3946394/1‑93 (MQ=255)
       ccAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAcc                                                                                  <  2:470405/93‑1 (MQ=255)
       ccAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAcc                                                                                  <  1:1340309/93‑1 (MQ=255)
       ccAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAcc                                                                                  <  1:3337081/93‑1 (MQ=255)
         agagGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGa                                                                                >  1:4371138/1‑93 (MQ=255)
           agGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGGCGTGGCGTGCCGACCGAGc                                                                              >  2:3210054/1‑93 (MQ=255)
            ggCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGcc                                                                             >  1:3567269/1‑93 (MQ=255)
              ccGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGcccc                                                                           <  1:2108097/93‑1 (MQ=255)
               cGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCg                                                                          >  1:4302091/1‑93 (MQ=255)
                 aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAg                                                                        <  2:2782027/93‑1 (MQ=255)
                 aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAg                                                                        <  1:3288899/93‑1 (MQ=255)
                  aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGt                                                                       >  2:874653/1‑93 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              <  2:1310487/93‑1 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              <  2:2032512/93‑1 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              >  1:1615815/1‑93 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              >  1:3491171/1‑93 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             >  2:1550942/1‑93 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             <  1:2645042/93‑1 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             >  2:4225065/1‑93 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            >  2:859257/1‑93 (MQ=255)
                              cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGatat                                                           <  1:2808503/93‑1 (MQ=255)
                              cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGatat                                                           <  2:1637110/93‑1 (MQ=255)
                              cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGatat                                                           >  1:4099420/1‑93 (MQ=255)
                                ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGGTGTCAGAGGTATCg                                                         >  2:1523113/1‑93 (MQ=255)
                                 gggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGt                                                        <  1:2753563/93‑1 (MQ=255)
                                   gAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg                                                      <  1:128252/93‑1 (MQ=255)
                                   gAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg                                                      >  1:569378/1‑93 (MQ=255)
                                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTgg                                                     <  1:2217543/93‑1 (MQ=255)
                                      tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTc                                                   <  2:3114953/93‑1 (MQ=255)
                                      tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTc                                                   <  1:526327/93‑1 (MQ=255)
                                      tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGGTGGTc                                                   >  2:1155230/1‑93 (MQ=255)
                                       cAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa                                                  <  2:1073328/93‑1 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 <  2:3526854/93‑1 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGCTGTCAGAGATATTGTTGGTCAg                                                 >  1:2129449/1‑93 (MQ=255)
                                         atcacatcccCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg                                                >  2:4574057/1‑93 (MQ=255)
                                          tcacatcacCGCGGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGa                                               <  1:3383420/93‑1 (MQ=255)
                                          tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGa                                               <  2:2977043/93‑1 (MQ=255)
                                          tcacatcacCGCCGGGCGGCAATTGATTCAGCCGAGCGGCGGTTGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGACATATCGTTGGTCAGGa                                               <  2:2319708/93‑1 (MQ=255)
                                           cacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt                                              <  2:332185/93‑1 (MQ=255)
                                           cacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt                                              <  2:1614681/93‑1 (MQ=255)
                                           cacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt                                              <  2:1348968/93‑1 (MQ=255)
                                            acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc                                             >  1:1474281/1‑93 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           <  1:997838/93‑1 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           <  2:1516147/93‑1 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           <  1:4567035/93‑1 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           >  2:1825900/1‑93 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           >  1:4428725/1‑93 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGCGGCGTGCTGACCGAGCCCCCAGTTGTCAGAGAGATCGTTGGGCAGGAtctc                                           >  1:1850447/1‑93 (MQ=255)
                                              atcacCGCCGTGCGGAAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGTCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc                                           <  2:2857962/93‑1 (MQ=255)
                                               tcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct                                          >  2:2390454/1‑93 (MQ=255)
                                               tcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct                                          >  2:594104/1‑93 (MQ=255)
                                                cacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtt                                         <  2:4504581/93‑1 (MQ=255)
                                                cacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtt                                         <  2:4642615/93‑1 (MQ=255)
                                                 cccgcggTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt                                        <  1:1725928/92‑1 (MQ=255)
                                                 acCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt                                        >  1:2321856/1‑93 (MQ=255)
                                                 acCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt                                        <  1:4275564/93‑1 (MQ=255)
                                                 acCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt                                        <  2:1926527/93‑1 (MQ=255)
                                                  ccgccgTGCGTGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       <  2:291156/93‑1 (MQ=255)
                                                  ccgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       >  2:770624/1‑93 (MQ=255)
                                                  ccgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       >  2:102851/1‑93 (MQ=255)
                                                  ccgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       >  2:2641641/1‑93 (MQ=255)
                                                  ccgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       <  2:3963147/93‑1 (MQ=255)
                                                    gccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTg                                     <  1:1098333/93‑1 (MQ=255)
                                                    gccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTg                                     <  2:2728708/93‑1 (MQ=255)
                                                     ccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGc                                    <  2:2505851/93‑1 (MQ=255)
                                                     ccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGc                                    >  1:2485287/1‑93 (MQ=255)
                                                     ccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGc                                    <  2:3654150/93‑1 (MQ=255)
                                                      cgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCACGATCTCTTTTTGCa                                   >  2:4460729/1‑93 (MQ=255)
                                                       gTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAg                                  >  1:1353373/1‑93 (MQ=255)
                                                       gTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAg                                  >  1:1983785/1‑93 (MQ=255)
                                                        ggCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGc                                 <  2:2953014/92‑1 (MQ=255)
                                                          ggggAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGccc                               <  1:3458694/92‑1 (MQ=255)
                                                          cGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGccc                               <  1:1800700/93‑1 (MQ=255)
                                                             gAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAg                            <  2:2578486/93‑1 (MQ=255)
                                                             gAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAg                            >  1:4459675/1‑93 (MQ=255)
                                                                 tGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTATTTTTGCAGCCCCAGCAAc                        >  1:843581/1‑93 (MQ=255)
                                                                   aTACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGc                      >  1:4494075/1‑93 (MQ=255)
                                                                      cAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCAtt                   <  2:176333/93‑1 (MQ=255)
                                                                        gCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTca                 >  2:4298050/1‑93 (MQ=255)
                                                                           gAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagca              <  2:3040661/93‑1 (MQ=255)
                                                                           gAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagca              <  1:3410599/93‑1 (MQ=255)
                                                                              cggcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGc           <  1:2976853/93‑1 (MQ=255)
                                                                              cggcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGc           >  2:2158119/1‑93 (MQ=255)
                                                                              cggcggTCGTGGCGTGCTGACCGAGACCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAAGCCTTCAGGAGGc           >  2:3284032/1‑93 (MQ=255)
                                                                                gcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTg         >  2:432683/1‑93 (MQ=255)
                                                                                gcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTg         >  2:4239476/1‑93 (MQ=255)
                                                                                gcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTg         >  2:2114632/1‑93 (MQ=255)
                                                                                  ggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGa       <  2:2268611/93‑1 (MQ=255)
                                                                                    tCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAtt     <  1:3037994/93‑1 (MQ=255)
                                                                                    tCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAtt     <  2:3894422/93‑1 (MQ=255)
                                                                                     cGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAttt    <  2:3112242/93‑1 (MQ=255)
                                                                                      gtggcgTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTg   >  1:4068514/1‑93 (MQ=255)
                                                                                       tggcgTGCTGCCCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGc  <  2:775758/93‑1 (MQ=255)
                                                                                       tggcgTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGc  >  1:2372709/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC  >  NC_000913/4388253‑4388432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  >  NC_000913/4388251‑4388436
                                                                                            |                                                                                             
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCG                                                                                              <  SRR4302535.570238/94‑1 (MQ=60)
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCG                                                                                              <  SRR4302535.2927521/94‑1 (MQ=60)
  CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTG                                                                                            <  SRR4302535.1615754/94‑1 (MQ=60)
    AGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCT                                                                                          <  SRR4302535.2463499/94‑1 (MQ=60)
     GGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTG                                                                                         >  SRR4302535.2204789/1‑94 (MQ=60)
     GGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTG                                                                                         >  SRR4302535.2204790/1‑94 (MQ=60)
       TGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAC                                                                                       >  SRR4302535.3973343/1‑94 (MQ=60)
        GCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACC                                                                                      <  SRR4302535.808809/94‑1 (MQ=60)
         CCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCG                                                                                     <  SRR4302535.3360320/94‑1 (MQ=60)
         CCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCG                                                                                     <  SRR4302535.1349872/94‑1 (MQ=60)
          CAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGA                                                                                    >  SRR4302535.2721642/1‑94 (MQ=60)
          CAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGA                                                                                    >  SRR4302535.4401048/1‑94 (MQ=60)
           AGAGGCCGAACTCACGCACTCCTGGGGAGTCCATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAG                                                                                   >  SRR4302535.2285020/1‑94 (MQ=60)
           AGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAG                                                                                   <  SRR4302535.4115250/94‑1 (MQ=60)
             AGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCC                                                                                 >  SRR4302535.332078/1‑94 (MQ=60)
             AGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCC                                                                                 >  SRR4302535.3592003/1‑94 (MQ=60)
              ggccaaactcacacactcctgggacatcggTCACATCACCGCCATGCGGGTTGTGATACAGCCGAGCGGCGGTCATGGTGTGCTGACCGAGCCC                                                                                <  SRR4302535.4716221/64‑1 (MQ=60)
               GCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCC                                                                               <  SRR4302535.3462541/94‑1 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              >  SRR4302535.4331524/1‑94 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              <  SRR4302535.2123301/94‑1 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            >  SRR4302535.1556419/1‑94 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            >  SRR4302535.1273759/1‑94 (MQ=60)
                   AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGT                                                                           <  SRR4302535.3311767/94‑1 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         <  SRR4302535.4374064/94‑1 (MQ=60)
                       CACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGATGTC                                                                       >  SRR4302535.392974/1‑94 (MQ=60)
                           CACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAG                                                                   >  SRR4302535.135977/1‑94 (MQ=60)
                           CACTCCTGCGGAGTCAACCGCGTCGCCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAG                                                                   <  SRR4302535.1963396/94‑1 (MQ=60)
                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  >  SRR4302535.1627460/1‑94 (MQ=60)
                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  >  SRR4302535.3515433/1‑94 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 >  SRR4302535.3410242/1‑94 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGTGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATA                                                                <  SRR4302535.2530509/94‑1 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATA                                                                <  SRR4302535.2663985/94‑1 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTACTGACCGAGCCCCGAGTTGTCAGAGATA                                                                <  SRR4302535.236830/94‑1 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               >  SRR4302535.4127486/1‑94 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               >  SRR4302535.4539804/1‑94 (MQ=60)
                                CTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATC                                                              >  SRR4302535.1725729/1‑94 (MQ=60)
                                CTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATC                                                              <  SRR4302535.2828471/94‑1 (MQ=60)
                                CTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATC                                                              <  SRR4302535.2818594/94‑1 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             >  SRR4302535.1325333/1‑94 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             >  SRR4302535.444415/1‑94 (MQ=60)
                                   GGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTT                                                           <  SRR4302535.3509163/94‑1 (MQ=60)
                                   GGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTT                                                           <  SRR4302535.2773185/94‑1 (MQ=60)
                                   GGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTT                                                           <  SRR4302535.2167252/94‑1 (MQ=60)
                                    GGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTG                                                          >  SRR4302535.573377/1‑94 (MQ=60)
                                     GAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGG                                                         <  SRR4302535.129156/94‑1 (MQ=60)
                                      AGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGT                                                        <  SRR4302535.2233324/94‑1 (MQ=60)
                                      AGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGC                                                        >  SRR4302535.561928/1‑94 (MQ=60)
                                       GTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTC                                                       >  SRR4302535.1438916/1‑94 (MQ=60)
                                        TCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCA                                                      >  SRR4302535.1763401/1‑94 (MQ=60)
                                        TCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCA                                                      <  SRR4302535.530002/94‑1 (MQ=60)
                                         CAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAG                                                     <  SRR4302535.867232/94‑1 (MQ=60)
                                         CAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAG                                                     >  SRR4302535.3515705/1‑94 (MQ=60)
                                         CAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGCTGTCAGAGATATTGTTGGTCAG                                                     >  SRR4302535.2144800/1‑94 (MQ=60)
                                            TCACATCACCGCGGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAT                                                  <  SRR4302535.3406941/94‑1 (MQ=60)
                                            TCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGGTGTCAGAGATATCGTTGGTCAGGGT                                                  >  SRR4302535.2466034/1‑94 (MQ=60)
                                             CACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATC                                                 >  SRR4302535.1484836/1‑94 (MQ=60)
                                             CACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATC                                                 >  SRR4302535.4465320/1‑94 (MQ=60)
                                               CATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC                                               >  SRR4302535.4459064/1‑94 (MQ=60)
                                               CATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGCGGCGTGCTGACCGAGCCCCCAGTTGTCAGAGAGATCGTTGGGCAGGATCTC                                               >  SRR4302535.1863783/1‑94 (MQ=60)
                                                ATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT                                              >  SRR4302535.1842095/1‑94 (MQ=60)
                                                ATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT                                              <  SRR4302535.1004920/94‑1 (MQ=60)
                                                ATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT                                              <  SRR4302535.4598188/94‑1 (MQ=60)
                                                  CACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTT                                            >  SRR4302535.2338472/1‑94 (MQ=60)
                                                   CCCGCGGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTT                                           <  SRR4302535.1738407/94‑1 (MQ=60)
                                                   ACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTT                                           <  SRR4302535.4304830/94‑1 (MQ=60)
                                                     CGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTG                                         <  SRR4302535.4005626/94‑1 (MQ=60)
                                                      GCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGC                                        <  SRR4302535.1106153/94‑1 (MQ=60)
                                                      GCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGC                                        >  SRR4302535.2503204/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302535.1363057/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302535.1998067/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302535.2140238/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302535.432066/1‑94 (MQ=60)
                                                          TGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCC                                    >  SRR4302535.4511763/1‑94 (MQ=60)
                                                            GGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCC                                  <  SRR4302535.3482741/94‑1 (MQ=60)
                                                            CGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCC                                  <  SRR4302535.1813717/94‑1 (MQ=60)
                                                              GGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAG                                >  SRR4302535.4490262/1‑94 (MQ=60)
                                                                  GTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTATTTTTGCAGCCCCAGCAAC                            >  SRR4302535.849508/1‑94 (MQ=60)
                                                                   TGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACG                           >  SRR4302535.4190342/1‑94 (MQ=60)
                                                                    GATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGC                          >  SRR4302535.4524923/1‑94 (MQ=60)
                                                                     ATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCA                         >  SRR4302535.3203269/1‑94 (MQ=60)
                                                                     ATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCA                         <  SRR4302535.338432/94‑1 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     <  SRR4302535.289978/94‑1 (MQ=60)
                                                                            CGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCA                  >  SRR4302535.2434322/1‑94 (MQ=60)
                                                                             GAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAG                 <  SRR4302535.3434330/94‑1 (MQ=60)
                                                                                CGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCT              <  SRR4302535.2997739/94‑1 (MQ=60)
                                                                                 GGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTG             <  SRR4302535.2051448/94‑1 (MQ=60)
                                                                                    GGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAT          <  SRR4302535.4634133/94‑1 (MQ=60)
                                                                                     GTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATT         <  SRR4302535.1622755/94‑1 (MQ=60)
                                                                                      TCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTT        <  SRR4302535.3059275/94‑1 (MQ=60)
                                                                                       CGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTG       >  SRR4302535.4096359/1‑94 (MQ=60)
                                                                                        GTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC      >  SRR4302535.2389751/1‑94 (MQ=60)
                                                                                         TGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCC     <  SRR4302535.3051099/94‑1 (MQ=60)
                                                                                          GGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCG    <  SRR4302535.3034081/94‑1 (MQ=60)
                                                                                           GCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGA   >  SRR4302535.3907518/1‑94 (MQ=60)
                                                                                            CGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  <  SRR4302535.2585389/94‑1 (MQ=60)
                                                                                            |                                                                                             
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  >  NC_000913/4388251‑4388436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑