Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,343 T→C T249A (ACC→GCC)  b4161 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,3430TC99.4% 1022.3 / ‑4.6 332T249A (ACC→GCC) b4161hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (153/177);  total (155/177)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 360 total aligned reads displayed.

CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC  >  NC_000913/4388253‑4388432
                                                                                          |                                                                                         
ccAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGgcgt                                                                                         >  1:1412663/1‑93 (MQ=255)
  aGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGc                                                                                       <  2:3829794/93‑1 (MQ=255)
   ggTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCt                                                                                      <  2:1962817/93‑1 (MQ=255)
   ggTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCt                                                                                      <  2:2233503/93‑1 (MQ=255)
    gTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGCCGTGGCGTGCTg                                                                                     >  2:880140/1‑93 (MQ=255)
     tGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGa                                                                                    >  1:7240608/1‑93 (MQ=255)
        cAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCg                                                                                 >  1:6857840/1‑93 (MQ=255)
        cAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCg                                                                                 >  2:5410797/1‑93 (MQ=255)
        cAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCg                                                                                 >  2:3439508/1‑93 (MQ=255)
        cAGAGGCCGAACTCACGCACTCCTGGGGAGTCAAGCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCg                                                                                 <  2:4235341/93‑1 (MQ=255)
           agGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGc                                                                              >  1:4638127/1‑93 (MQ=255)
           agGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGc                                                                              <  1:374549/93‑1 (MQ=255)
            ggCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGcc                                                                             >  1:3574225/1‑93 (MQ=255)
            ggCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGcc                                                                             <  1:2109112/93‑1 (MQ=255)
             gCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGccc                                                                            <  2:7711758/93‑1 (MQ=255)
              ccGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGcccc                                                                           <  2:5541869/93‑1 (MQ=255)
               cGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCg                                                                          >  1:5204512/1‑93 (MQ=255)
                gAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCgg                                                                         >  2:7106920/1‑92 (MQ=255)
                gAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGa                                                                         <  1:829770/93‑1 (MQ=255)
                gAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGa                                                                         <  1:5338848/93‑1 (MQ=255)
                 aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCgcg                                                                        >  1:6014783/1‑91 (MQ=255)
                 aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAg                                                                        >  2:1893221/1‑93 (MQ=255)
                  aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGt                                                                       >  1:3078285/1‑93 (MQ=255)
                  aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGt                                                                       <  2:2438800/93‑1 (MQ=255)
                   cTCCCGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGtt                                                                      <  1:1271760/93‑1 (MQ=255)
                   cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGtt                                                                      >  2:2662734/1‑93 (MQ=255)
                    tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTg                                                                     <  2:3608734/93‑1 (MQ=255)
                       cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCa                                                                  <  2:6721278/93‑1 (MQ=255)
                       cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCa                                                                  <  1:876336/93‑1 (MQ=255)
                        gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCag                                                                 >  2:145454/1‑93 (MQ=255)
                          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCagag                                                               >  1:1074776/1‑93 (MQ=255)
                          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCagag                                                               >  1:5452448/1‑93 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              <  1:4314986/93‑1 (MQ=255)
                           cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAgaga                                                              >  1:7446241/1‑93 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             >  2:3018883/1‑93 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             >  1:6589369/1‑93 (MQ=255)
                            tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGat                                                             <  1:3098410/93‑1 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            <  1:6253363/93‑1 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            <  2:474014/93‑1 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            >  1:5832634/1‑93 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            <  2:7418107/93‑1 (MQ=255)
                             ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGata                                                            <  2:6570829/93‑1 (MQ=255)
                              cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGatat                                                           <  1:3342707/93‑1 (MQ=255)
                               tGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                                                          <  2:5964396/93‑1 (MQ=255)
                               tGGGGAGTCAATCACATCACCGCTGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                                                          <  2:5361312/93‑1 (MQ=255)
                               tGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                                                          <  2:159245/93‑1 (MQ=255)
                                ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCg                                                         <  1:1742931/93‑1 (MQ=255)
                                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                                                       <  2:7935206/93‑1 (MQ=255)
                                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                                                       >  2:6041694/1‑93 (MQ=255)
                                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                                                       <  1:7394676/93‑1 (MQ=255)
                                    aGTCAATCACACCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTgg                                                     <  1:4079806/93‑1 (MQ=255)
                                     gTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGGTATCGTTGGt                                                    >  2:6904109/1‑93 (MQ=255)
                                      tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTc                                                   >  1:2441111/1‑93 (MQ=255)
                                      tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGGTGTCAGAGATATCGTTGGTc                                                   >  1:7166586/1‑93 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 >  1:3217739/1‑93 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 >  2:7867983/1‑93 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 <  2:5190352/93‑1 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 <  2:3254549/93‑1 (MQ=255)
                                        aaTCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg                                                 >  2:7282306/1‑93 (MQ=255)
                                         atcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCCTGGCGTGCTGACGGAGCCCGGCGGTGTCAGAGATATCTTTGGGCAga                                                >  1:2695235/1‑92 (MQ=255)
                                             catcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct                                            >  2:2332621/1‑93 (MQ=255)
                                             catcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct                                            >  2:6102066/1‑93 (MQ=255)
                                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGGTGGTCAGGAtctt                                           >  2:4598493/1‑92 (MQ=255)
                                                cacCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtt                                         >  1:6506210/1‑93 (MQ=255)
                                                 acCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt                                        >  2:5258211/1‑93 (MQ=255)
                                                  ccgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtttt                                       <  1:6310237/93‑1 (MQ=255)
                                                   cgccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttttt                                      <  2:3972020/93‑1 (MQ=255)
                                                    gccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTg                                     <  2:7736738/93‑1 (MQ=255)
                                                     ccgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGc                                    <  2:570144/93‑1 (MQ=255)
                                                      cgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCa                                   <  1:2227406/93‑1 (MQ=255)
                                                      cgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCa                                   <  2:3108008/93‑1 (MQ=255)
                                                      cgTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCa                                   <  1:7360946/93‑1 (MQ=255)
                                                       gTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAg                                  <  2:2801603/93‑1 (MQ=255)
                                                        tGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGGAGc                                 >  1:94570/1‑93 (MQ=255)
                                                           gggAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGcccc                              <  2:6273836/93‑1 (MQ=255)
                                                            ggccgTGCTACGGCCGAGCGGCGGTCGTGGCGTGCTGCCCGAGCCCCGAGTTGTCGGAGGTCTCGTTGGTCAGGATCTCTTTTTGCCGCCCCa                             <  1:6998488/89‑1 (MQ=255)
                                                            ggAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCa                             <  1:4963307/93‑1 (MQ=255)
                                                              aaGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGc                           >  2:4257866/1‑93 (MQ=255)
                                                                gTGATACAGCGGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCaa                         <  1:4343869/93‑1 (MQ=255)
                                                                gTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCaa                         <  2:1209443/93‑1 (MQ=255)
                                                                gTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCaa                         <  2:5134681/93‑1 (MQ=255)
                                                                 tGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                                                       >  1:7902855/1‑62 (MQ=255)
                                                                 tGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAc                        >  2:2155564/1‑93 (MQ=255)
                                                                 tGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAc                        <  2:2549798/93‑1 (MQ=255)
                                                                       aGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTc                  <  2:3913001/93‑1 (MQ=255)
                                                                        gCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTca                 >  1:4197985/1‑93 (MQ=255)
                                                                        gCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGAAGCCCCCGCAACGCATTca                 >  1:6780979/1‑93 (MQ=255)
                                                                        gCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTAGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTca                 <  1:1927418/93‑1 (MQ=255)
                                                                          cGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagc               >  1:2866010/1‑93 (MQ=255)
                                                                           gAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagca              >  2:1091343/1‑93 (MQ=255)
                                                                           gAGCGGCGGTCGGGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagca              <  1:1626052/93‑1 (MQ=255)
                                                                            aGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTcagcag             <  1:4827127/93‑1 (MQ=255)
                                                                               ggcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCt          >  2:1712717/1‑93 (MQ=255)
                                                                               ggcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCt          <  2:4937818/93‑1 (MQ=255)
                                                                                gcggTCTTGGCGTGCTGACCGAGCCACGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTg         <  1:7791905/93‑1 (MQ=255)
                                                                                gcggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTg         >  1:3881463/1‑93 (MQ=255)
                                                                                  ggTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGa       <  2:6391165/93‑1 (MQ=255)
                                                                                     cGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAttt    <  1:7677267/93‑1 (MQ=255)
                                                                                     cGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAttt    <  1:5675236/93‑1 (MQ=255)
                                                                                     cGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAttt    <  1:217594/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC  >  NC_000913/4388253‑4388432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  >  NC_000913/4388251‑4388436
                                                                                            |                                                                                             
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGTAGTCAATCACATCAGTGCGGGGCGGGAAGTTATACAGCCGAGCGGCGGTCGTGGCG                                                                                              <  SRR4302543.2729202/94‑1 (MQ=60)
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCG                                                                                              >  SRR4302543.4376055/1‑94 (MQ=60)
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCG                                                                                              >  SRR4302543.7251964/1‑94 (MQ=60)
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGGGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCG                                                                                              <  SRR4302543.4546745/94‑1 (MQ=60)
 TCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGT                                                                                             >  SRR4302543.1425436/1‑94 (MQ=60)
  CCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTG                                                                                            <  SRR4302543.7593882/94‑1 (MQ=60)
    caGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCT                                                                                          <  SRR4302543.1828835/92‑1 (MQ=60)
    AGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCT                                                                                          <  SRR4302543.4789615/94‑1 (MQ=60)
     GGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTG                                                                                         >  SRR4302543.4978648/1‑94 (MQ=60)
      GTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGA                                                                                        <  SRR4302543.7144563/94‑1 (MQ=60)
      GTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGA                                                                                        >  SRR4302543.7310692/1‑94 (MQ=60)
       TGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGAC                                                                                       >  SRR4302543.6022865/1‑94 (MQ=60)
         CCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCG                                                                                     >  SRR4302543.6922488/1‑94 (MQ=60)
         CCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCG                                                                                     <  SRR4302543.7057137/94‑1 (MQ=60)
          CAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGA                                                                                    >  SRR4302543.5218578/1‑94 (MQ=60)
          CAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGA                                                                                    >  SRR4302543.5418394/1‑94 (MQ=60)
            GAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGC                                                                                  >  SRR4302543.7839264/1‑94 (MQ=60)
            GAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGC                                                                                  >  SRR4302543.4680310/1‑94 (MQ=60)
             AGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCC                                                                                 >  SRR4302543.3605815/1‑94 (MQ=60)
             AGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCC                                                                                 <  SRR4302543.377998/94‑1 (MQ=60)
              GGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCC                                                                                <  SRR4302543.2127990/94‑1 (MQ=60)
              GGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCC                                                                                >  SRR4302543.6059684/1‑94 (MQ=60)
               GCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCC                                                                               <  SRR4302543.835461/94‑1 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              >  SRR4302543.3178009/1‑94 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              >  SRR4302543.7753909/1‑94 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              >  SRR4302543.5251428/1‑94 (MQ=60)
                CCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCG                                                                              >  SRR4302543.3055029/1‑94 (MQ=60)
                 CGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGA                                                                             >  SRR4302543.6874152/1‑94 (MQ=60)
                  gaacccacgctgtctTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            <  SRR4302543.1555349/79‑1 (MQ=60)
                  GAACTCGCGCACTCATAGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            <  SRR4302543.7926280/94‑1 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGCG                                                                            >  SRR4302543.6070380/1‑94 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            <  SRR4302543.5387138/94‑1 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            <  SRR4302543.4896154/94‑1 (MQ=60)
                  GAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAG                                                                            <  SRR4302543.837083/94‑1 (MQ=60)
                   AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGT                                                                           >  SRR4302543.3105966/1‑94 (MQ=60)
                   AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGT                                                                           >  SRR4302543.1378684/1‑94 (MQ=60)
                    ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTT                                                                          >  SRR4302543.2217275/1‑94 (MQ=60)
                    ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTT                                                                          <  SRR4302543.5369140/94‑1 (MQ=60)
                     CTCCCGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         <  SRR4302543.1283120/94‑1 (MQ=60)
                     CTCACGCCCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         <  SRR4302543.997937/94‑1 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.7278394/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.3664090/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.1646128/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.2247743/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.6349973/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.6645584/1‑94 (MQ=60)
                     CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTG                                                                         >  SRR4302543.2149116/1‑94 (MQ=60)
                      TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGT                                                                        <  SRR4302543.1888538/94‑1 (MQ=60)
                      TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGT                                                                        <  SRR4302543.6918411/94‑1 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     <  SRR4302543.419945/94‑1 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     >  SRR4302543.562876/1‑94 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     <  SRR4302543.932871/94‑1 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     <  SRR4302543.884129/94‑1 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     <  SRR4302543.7313040/94‑1 (MQ=60)
                         CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAG                                                                     <  SRR4302543.214689/94‑1 (MQ=60)
                           CACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAG                                                                   >  SRR4302543.1084435/1‑94 (MQ=60)
                           CACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAG                                                                   >  SRR4302543.5501892/1‑94 (MQ=60)
                            CCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  <  SRR4302543.6267453/94‑1 (MQ=60)
                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  >  SRR4302543.7434245/1‑94 (MQ=60)
                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  >  SRR4302543.7517599/1‑94 (MQ=60)
                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGA                                                                  >  SRR4302543.7434246/1‑94 (MQ=60)
                             ncnCTGGNGNGTCAATCACANNNNCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.2227329/91‑1 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.3129057/94‑1 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 >  SRR4302543.6651451/1‑94 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.3291386/94‑1 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.3866011/94‑1 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.3866012/94‑1 (MQ=60)
                             CTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGAT                                                                 <  SRR4302543.4354185/94‑1 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATA                                                                >  SRR4302543.5885684/1‑94 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATA                                                                <  SRR4302543.3126280/94‑1 (MQ=60)
                              TCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATA                                                                >  SRR4302543.6566161/1‑94 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               <  SRR4302543.110355/94‑1 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               <  SRR4302543.4223313/94‑1 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               <  SRR4302543.6312351/94‑1 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               <  SRR4302543.1950492/94‑1 (MQ=60)
                               CCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATAT                                                               >  SRR4302543.1636268/1‑94 (MQ=60)
                                CTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATC                                                              >  SRR4302543.2803144/1‑94 (MQ=60)
                                CTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATC                                                              <  SRR4302543.3372807/94‑1 (MQ=60)
                                 TGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             <  SRR4302543.2707874/94‑1 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             <  SRR4302543.3923037/94‑1 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             <  SRR4302543.3923036/94‑1 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             >  SRR4302543.3114048/1‑94 (MQ=60)
                                 TGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCG                                                             <  SRR4302543.7218961/94‑1 (MQ=60)
                                  TGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGT                                                            <  SRR4302543.4562466/94‑1 (MQ=60)
                                  GGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGT                                                            <  SRR4302543.5466970/94‑1 (MQ=60)
                                  GGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGT                                                            <  SRR4302543.1758483/94‑1 (MQ=60)
                                   GGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTT                                                           >  SRR4302543.4273929/1‑94 (MQ=60)
                                   GGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTT                                                           <  SRR4302543.2946653/94‑1 (MQ=60)
                                    GGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTG                                                          >  SRR4302543.2130914/1‑94 (MQ=60)
                                    GGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTG                                                          >  SRR4302543.3290281/1‑94 (MQ=60)
                                    GGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTG                                                          <  SRR4302543.7465694/94‑1 (MQ=60)
                                    GGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTG                                                          >  SRR4302543.7512043/1‑94 (MQ=60)
                                     GAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGG                                                         >  SRR4302543.2205196/1‑94 (MQ=60)
                                     GAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGG                                                         <  SRR4302543.3498434/94‑1 (MQ=60)
                                      AGTCAATCACACCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGT                                                        <  SRR4302543.4116376/94‑1 (MQ=60)
                                       GTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTC                                                       >  SRR4302543.2463114/1‑94 (MQ=60)
                                       GTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGGTGTCAGAGATATCGTTGGTC                                                       >  SRR4302543.7236195/1‑94 (MQ=60)
                                       GTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGACCCGAGTTGTCAGAGATATCGTTGGTC                                                       >  SRR4302543.5008065/1‑94 (MQ=60)
                                        TCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCA                                                      >  SRR4302543.7993398/1‑94 (MQ=60)
                                        TCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCA                                                      <  SRR4302543.5321764/94‑1 (MQ=60)
                                         CAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAG                                                     >  SRR4302543.7851926/1‑94 (MQ=60)
                                         CAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAG                                                     >  SRR4302543.3246811/1‑94 (MQ=60)
                                          AATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCCTGGCGTGCTGACGGAGCCCGGCGGTGTCAGAGATATCtttgggcaga                                                    >  SRR4302543.2719672/1‑84 (MQ=60)
                                           ATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGA                                                   <  SRR4302543.4834245/94‑1 (MQ=60)
                                            TCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAT                                                  <  SRR4302543.7289035/94‑1 (MQ=60)
                                              CCATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCT                                                <  SRR4302543.1684040/94‑1 (MQ=60)
                                               CATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC                                               >  SRR4302543.1708854/1‑94 (MQ=60)
                                                ATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGATCCCCTAGTTGTGCGAGATATCGTTGGTCAGGATCTCT                                              >  SRR4302543.2220317/1‑94 (MQ=60)
                                                ATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT                                              >  SRR4302543.6724493/1‑94 (MQ=60)
                                                 TCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTT                                             <  SRR4302543.3299112/94‑1 (MQ=60)
                                                 TCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTT                                             >  SRR4302543.887023/1‑94 (MQ=60)
                                                 TCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTT                                             >  SRR4302543.6567504/1‑94 (MQ=60)
                                                   ACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTT                                           <  SRR4302543.2509500/94‑1 (MQ=60)
                                                   ACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTT                                           <  SRR4302543.7938873/94‑1 (MQ=60)
                                                    ccgctggggtggagGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTT                                          <  SRR4302543.6738543/80‑1 (MQ=60)
                                                    CCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTT                                          >  SRR4302543.1536181/1‑94 (MQ=60)
                                                    CCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTT                                          <  SRR4302543.6369582/94‑1 (MQ=60)
                                                    CCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTT                                          <  SRR4302543.4754039/94‑1 (MQ=60)
                                                     CGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTG                                         >  SRR4302543.873296/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGGTATCGTTGGTCAGGGTCTCTTTTTGCAG                                      >  SRR4302543.4373536/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      <  SRR4302543.2247519/94‑1 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      <  SRR4302543.827458/94‑1 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302543.4151172/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      <  SRR4302543.7431733/94‑1 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAG                                      >  SRR4302543.5544612/1‑94 (MQ=60)
                                                        CGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGGTGTCAGAGgaatcgtggggcgggagctcttttttcac                                      >  SRR4302543.4373537/1‑65 (MQ=60)
                                                         GTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGGAGC                                     >  SRR4302543.95450/1‑94 (MQ=60)
                                                          TGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCC                                    <  SRR4302543.3606400/94‑1 (MQ=60)
                                                           GCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCC                                   <  SRR4302543.4358737/94‑1 (MQ=60)
                                                            CGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCC                                  <  SRR4302543.854449/94‑1 (MQ=60)
                                                            CGGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCC                                  >  SRR4302543.2544401/1‑94 (MQ=60)
                                                             GGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGTAGCCCCA                                 >  SRR4302543.2353369/1‑94 (MQ=60)
                                                             GGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCA                                 >  SRR4302543.7739027/1‑94 (MQ=60)
                                                             GGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCA                                 <  SRR4302543.6415256/94‑1 (MQ=60)
                                                             GGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCA                                 <  SRR4302543.668125/94‑1 (MQ=60)
                                                             GGGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCA                                 <  SRR4302543.6415255/94‑1 (MQ=60)
                                                              ggccgtgctacgGCCGAGCGGCGGTCGTGGCGTGCTGCCCGAGCCCCGAGTTGTCGGAGGTCTCGTTGGTCAGGATCTCTTTTTGCCGCCCCAG                                <  SRR4302543.7064668/82‑1 (MQ=60)
                                                              GGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAG                                <  SRR4302543.4579514/94‑1 (MQ=60)
                                                              GGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAG                                <  SRR4302543.7151367/94‑1 (MQ=60)
                                                              GGAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAG                                <  SRR4302543.5008487/94‑1 (MQ=60)
                                                               GAAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGC                               >  SRR4302543.7431532/1‑94 (MQ=60)
                                                                AAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCA                              >  SRR4302543.3376063/1‑94 (MQ=60)
                                                                AAGTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCA                              <  SRR4302543.4274983/94‑1 (MQ=60)
                                                                  GTGATACAGCGGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAC                            <  SRR4302543.4383264/94‑1 (MQ=60)
                                                                  GTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGagagcggagatcggaagagcggttcagcag                            >  SRR4302543.7977131/1‑64 (MQ=60)
                                                                  GTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAC                            <  SRR4302543.538693/94‑1 (MQ=60)
                                                                  GTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAC                            >  SRR4302543.409600/1‑94 (MQ=60)
                                                                  GTGATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAAC                            <  SRR4302543.3128966/94‑1 (MQ=60)
                                                                     ATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCA                         >  SRR4302543.1779078/1‑94 (MQ=60)
                                                                     ATACAGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCA                         >  SRR4302543.6264944/1‑94 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     >  SRR4302543.4236044/1‑94 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     <  SRR4302543.3654964/94‑1 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     <  SRR4302543.6979673/94‑1 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     >  SRR4302543.2006528/1‑94 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     >  SRR4302543.7571184/1‑94 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCA                     <  SRR4302543.7454085/94‑1 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGAAGCCCCCGCAACGCATTCA                     >  SRR4302543.6845014/1‑94 (MQ=60)
                                                                         AGCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTCTGCAGCCCCAGCAACGCATTCA                     <  SRR4302543.1399725/94‑1 (MQ=60)
                                                                          GCCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTAGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAG                    <  SRR4302543.1944777/94‑1 (MQ=60)
                                                                           CCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGC                   >  SRR4302543.2892134/1‑94 (MQ=60)
                                                                           CCGAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGC                   >  SRR4302543.2892133/1‑94 (MQ=60)
                                                                             GAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATCTCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAG                 <  SRR4302543.6866424/94‑1 (MQ=60)
                                                                             GAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAG                 >  SRR4302543.1192542/1‑94 (MQ=60)
                                                                             GAGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAG                 <  SRR4302543.6634298/94‑1 (MQ=60)
                                                                             GAGCGGCGGTCGGGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAG                 <  SRR4302543.1640538/94‑1 (MQ=60)
                                                                              AGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGGTATCGTTGGTCAGGATCTCTTTTTGCAGTCCCAGCAACGCATTCAGCAGG                >  SRR4302543.184887/1‑94 (MQ=60)
                                                                              AGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGG                <  SRR4302543.4871175/94‑1 (MQ=60)
                                                                              AGCGGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGG                <  SRR4302543.6668801/94‑1 (MQ=60)
                                                                                 GGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTG             <  SRR4302543.3035320/94‑1 (MQ=60)
                                                                                 GGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTG             >  SRR4302543.3915732/1‑94 (MQ=60)
                                                                                 GGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTG             >  SRR4302543.7481050/1‑94 (MQ=60)
                                                                                  GCGGTCTTGGCGTGCTGACCGAGCCACGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGG            <  SRR4302543.7865342/94‑1 (MQ=60)
                                                                                   GGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGA           <  SRR4302543.5828653/94‑1 (MQ=60)
                                                                                   CGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGA           <  SRR4302543.5828427/94‑1 (MQ=60)
                                                                                   CGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGA           >  SRR4302543.2259090/1‑94 (MQ=60)
                                                                                   CGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGA           <  SRR4302543.5757390/94‑1 (MQ=60)
                                                                                    GGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAT          >  SRR4302543.6320995/1‑94 (MQ=60)
                                                                                    GGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAT          >  SRR4302543.3119051/1‑94 (MQ=60)
                                                                                    GGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGAT          <  SRR4302543.7308459/94‑1 (MQ=60)
                                                                                      TCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTT        <  SRR4302543.5572012/94‑1 (MQ=60)
                                                                                       CGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTG       <  SRR4302543.219470/94‑1 (MQ=60)
                                                                                       CGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTG       <  SRR4302543.5727044/94‑1 (MQ=60)
                                                                                       CGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTG       <  SRR4302543.7749995/94‑1 (MQ=60)
                                                                                        GTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC      >  SRR4302543.911610/1‑94 (MQ=60)
                                                                                        GTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGC      >  SRR4302543.5111795/1‑94 (MQ=60)
                                                                                         TGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCC     >  SRR4302543.5211397/1‑94 (MQ=60)
                                                                                          GGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCG    >  SRR4302543.2096889/1‑94 (MQ=60)
                                                                                            CGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  <  SRR4302543.4679252/94‑1 (MQ=60)
                                                                                            CGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  <  SRR4302543.2758324/94‑1 (MQ=60)
                                                                                            |                                                                                             
CTCCAGGTGCCAGAGGCCGAACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTTTTGCAGCCCCAGCAACGCATTCAGCAGGCTGGATTTGCCGAC  >  NC_000913/4388251‑4388436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑