Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,395,834 C→G A282G (GCC→GGC)  mutL → MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,8340CG93.4% 526.9 / 24.5 181A282G (GCC→GGC) mutLMutL
Reads supporting (aligned to +/- strand):  ref base C (7/5);  new base G (85/84);  total (92/89)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

Only 100 of 196 total aligned reads displayed.

GAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTT  >  NC_000913/4395743‑4395923
                                                                                           |                                                                                         
gAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGcc                                                                                          >  1:3256230/1‑93 (MQ=255)
    ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATc                                                                                      >  1:3908792/1‑93 (MQ=255)
    ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATc                                                                                      <  2:17802/93‑1 (MQ=255)
       aGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGc                                                                                   >  1:2555099/1‑93 (MQ=255)
        gTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCa                                                                                  >  2:3276445/1‑93 (MQ=255)
        gTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCAAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCa                                                                                  >  1:1890621/1‑93 (MQ=255)
          aTTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAAc                                                                                <  1:3420598/93‑1 (MQ=255)
           ttGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAAcc                                                                               <  2:2362545/93‑1 (MQ=255)
           ttGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAAcc                                                                               >  2:3574030/1‑93 (MQ=255)
            tGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCg                                                                              <  2:291497/93‑1 (MQ=255)
              cTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGc                                                                            >  1:4436901/1‑93 (MQ=255)
               tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCa                                                                           >  2:3674741/1‑93 (MQ=255)
               tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCa                                                                           >  1:3954738/1‑93 (MQ=255)
               tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCa                                                                           >  2:2030693/1‑93 (MQ=255)
               tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCa                                                                           >  2:151944/1‑93 (MQ=255)
                 cGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCAtt                                                                         <  1:4170722/93‑1 (MQ=255)
                   tGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGTAGACAAACTAAGGGAAGATAAACAAGCGGGATTtg                                                                       >  2:4101927/1‑93 (MQ=255)
                   tGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtg                                                                       <  1:1690292/93‑1 (MQ=255)
                   tGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtg                                                                       <  1:1509208/93‑1 (MQ=255)
                    gAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtgt                                                                      <  1:2940893/93‑1 (MQ=255)
                    gAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtgt                                                                      <  2:4275573/93‑1 (MQ=255)
                    gAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtgt                                                                      <  2:1183087/93‑1 (MQ=255)
                     aaCGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTtgtg                                                                     <  2:2708232/93‑1 (MQ=255)
                      aCGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTgtgt                                                                    <  2:1239075/93‑1 (MQ=255)
                      aCGGTCCCATGATGCGCGACCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTgtgt                                                                    <  2:2997737/93‑1 (MQ=255)
                       cGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGtgtt                                                                   >  2:500162/1‑93 (MQ=255)
                        ggTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGtgttg                                                                  <  1:2124052/93‑1 (MQ=255)
                        ggTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGtgttg                                                                  >  2:2113805/1‑93 (MQ=255)
                         gTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGtgttgt                                                                 >  2:2449081/1‑93 (MQ=255)
                          tCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTa                                                                <  1:846324/93‑1 (MQ=255)
                          tCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTa                                                                <  1:3824381/93‑1 (MQ=255)
                           cGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAt                                                               >  1:2698464/1‑93 (MQ=255)
                            gCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATc                                                              >  1:2915332/1‑93 (MQ=255)
                            ccATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                                                              <  2:699078/92‑1 (MQ=255)
                             cATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCt                                                             <  1:2443318/93‑1 (MQ=255)
                             cATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCt                                                             >  1:2018179/1‑93 (MQ=255)
                             cATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCt                                                             <  2:3956224/93‑1 (MQ=255)
                              atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTg                                                            >  1:2291587/1‑93 (MQ=255)
                              atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTg                                                            >  1:3601576/1‑93 (MQ=255)
                              atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTg                                                            >  1:3549672/1‑93 (MQ=255)
                              atgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTg                                                            >  2:2801838/1‑93 (MQ=255)
                               tgatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTgg                                                           >  1:2670504/1‑93 (MQ=255)
                                gatgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGga                                                          <  2:746713/93‑1 (MQ=255)
                                 atgCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGgag                                                         <  1:127042/93‑1 (MQ=255)
                                    cgcgATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATc                                                      <  2:2318339/93‑1 (MQ=255)
                                    cgcgATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATc                                                      <  2:4239595/93‑1 (MQ=255)
                                      cgATCGCCTGATCAATCACGCGATCCTCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGa                                                    <  1:1962395/93‑1 (MQ=255)
                                       gATCGCCTGATCAATCAGGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAc                                                   <  1:4226025/93‑1 (MQ=255)
                                       gATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAc                                                   <  2:4625494/93‑1 (MQ=255)
                                       gATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAc                                                   >  2:2528213/1‑93 (MQ=255)
                                        aTCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAcc                                                  >  2:24331/1‑93 (MQ=255)
                                        aTCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAcc                                                  <  1:1621589/93‑1 (MQ=255)
                                        aTCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAcc                                                  <  2:1061658/93‑1 (MQ=255)
                                           gCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCcac                                               >  2:2626621/1‑93 (MQ=255)
                                              tGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc                                            >  2:2749681/1‑93 (MQ=255)
                                              tGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc                                            >  1:1785701/1‑93 (MQ=255)
                                              tGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc                                            <  2:3450407/93‑1 (MQ=255)
                                               gATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa                                           >  2:3158307/1‑93 (MQ=255)
                                               gATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa                                           >  1:321669/1‑93 (MQ=255)
                                                  caatcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGt                                        <  1:3944979/93‑1 (MQ=255)
                                                  caaccaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGt                                        <  1:3987020/93‑1 (MQ=255)
                                                   aatcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTg                                       <  2:4440793/93‑1 (MQ=255)
                                                    atcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTgg                                      >  2:3236313/1‑93 (MQ=255)
                                                     tcaCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGa                                     >  1:4544444/1‑93 (MQ=255)
                                                       aCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACg                                   >  1:2170226/1‑93 (MQ=255)
                                                        cgcgATCCGCCAGGCCTGCGAAGACAAACTGGGAGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGt                                  <  1:647629/93‑1 (MQ=255)
                                                         gcgATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTc                                 <  1:11246/93‑1 (MQ=255)
                                                         gcgATCCGCCAGGCCCGGGAAGACAAACTGGCGGGCGATCAGCAACCGGCATTTGTGGTGTATCTGGAGATCGACACACATCACGTGGACgcc                                 >  2:563910/1‑91 (MQ=255)
                                                          ggATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAGCCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCa                                <  1:2636457/92‑1 (MQ=255)
                                                           gATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCaa                               >  1:3716275/1‑93 (MQ=255)
                                                           gATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCaa                               <  1:2346336/93‑1 (MQ=255)
                                                           gATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCaa                               >  2:483103/1‑93 (MQ=255)
                                                            aTCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAAc                              >  1:620627/1‑93 (MQ=255)
                                                              ccGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGt                            >  1:3063343/1‑93 (MQ=255)
                                                              ccGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGt                            <  1:4143914/93‑1 (MQ=255)
                                                                gCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGc                          >  2:1950912/1‑93 (MQ=255)
                                                                  cAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAc                        >  2:3680191/1‑93 (MQ=255)
                                                                    ggCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAccc                      <  2:1646355/93‑1 (MQ=255)
                                                                    ggCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAccc                      >  1:1818698/1‑93 (MQ=255)
                                                                    ggCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAccc                      >  1:3632813/1‑93 (MQ=255)
                                                                     gCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAcccc                     <  1:2313992/93‑1 (MQ=255)
                                                                     gCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAcccc                     <  1:1735237/93‑1 (MQ=255)
                                                                       cTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGc                   >  2:155038/1‑93 (MQ=255)
                                                                            aaGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAac              <  2:404895/93‑1 (MQ=255)
                                                                             aGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAaca             <  1:2479047/93‑1 (MQ=255)
                                                                              gACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAacac            >  2:640985/1‑93 (MQ=255)
                                                                                 aaaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGac         >  2:3525723/1‑92 (MQ=255)
                                                                                 aaaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGaa         <  2:1321932/93‑1 (MQ=255)
                                                                                 aaaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGaa         <  1:1389513/93‑1 (MQ=255)
                                                                                 aaaCTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGaa         <  1:3972653/93‑1 (MQ=255)
                                                                                  aaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAg        <  2:88890/93‑1 (MQ=255)
                                                                                  aaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAg        <  1:286579/93‑1 (MQ=255)
                                                                                  aaCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAg        <  1:4334712/93‑1 (MQ=255)
                                                                                   aCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAgg       >  1:3448052/1‑92 (MQ=255)
                                                                                   aCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGt       <  1:2771263/93‑1 (MQ=255)
                                                                                   aCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGt       <  2:2183191/93‑1 (MQ=255)
                                                                                    cTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTg      <  2:2864789/93‑1 (MQ=255)
                                                                                     tGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCCAACACGAAGTGc     >  2:4340850/1‑93 (MQ=255)
                                                                                     tGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGc     <  1:1369630/93‑1 (MQ=255)
                                                                                        ggggCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGtt  <  1:525438/93‑1 (MQ=255)
                                                                                           |                                                                                         
GAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTT  >  NC_000913/4395743‑4395923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTTTCC  >  NC_000913/4395741‑4395926
                                                                                             |                                                                                            
CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCTATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGG                                                                                              >  SRR4302535.409919/1‑94 (MQ=60)
CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCTATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGG                                                                                              >  SRR4302535.409918/1‑94 (MQ=60)
 AGAAATTCAGTATTGCTACGTGAACGGTCGCATTATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGC                                                                                             >  SRR4302535.1526766/1‑94 (MQ=60)
 AGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCC                                                                                             >  SRR4302535.3278855/1‑94 (MQ=60)
  GAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCG                                                                                            <  SRR4302535.4240475/94‑1 (MQ=60)
    AATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGAT                                                                                          >  SRR4302535.357987/1‑94 (MQ=60)
     ATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATC                                                                                         >  SRR4302535.3935564/1‑94 (MQ=60)
        CAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGC                                                                                      >  SRR4302535.2573500/1‑94 (MQ=60)
        CAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGC                                                                                      <  SRR4302535.3982730/94‑1 (MQ=60)
         AGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCAAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCA                                                                                     >  SRR4302535.1904240/1‑94 (MQ=60)
          GTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAA                                                                                    >  SRR4302535.2183067/1‑94 (MQ=60)
           TATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAAC                                                                                   <  SRR4302535.2753503/94‑1 (MQ=60)
            ATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACC                                                                                  <  SRR4302535.2683494/94‑1 (MQ=60)
            ATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACC                                                                                  <  SRR4302535.3444387/94‑1 (MQ=60)
               GCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGC                                                                               >  SRR4302535.4639484/1‑94 (MQ=60)
               GCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGC                                                                               >  SRR4302535.4467309/1‑94 (MQ=60)
                CTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCA                                                                              >  SRR4302535.3981734/1‑94 (MQ=60)
                   CGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTT                                                                           <  SRR4302535.4199280/94‑1 (MQ=60)
                   CGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTT                                                                           >  SRR4302535.2709916/1‑94 (MQ=60)
                     TGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGT                                                                         <  SRR4302535.1702525/94‑1 (MQ=60)
                     TGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGT                                                                         <  SRR4302535.1520082/94‑1 (MQ=60)
                      GAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTG                                                                        <  SRR4302535.1796154/94‑1 (MQ=60)
                      GAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTG                                                                        <  SRR4302535.2961570/94‑1 (MQ=60)
                       AACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGT                                                                       >  SRR4302535.3560279/1‑94 (MQ=60)
                        ACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTT                                                                      >  SRR4302535.173525/1‑94 (MQ=60)
                          GGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGT                                                                    <  SRR4302535.2139362/94‑1 (MQ=60)
                            TCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTAT                                                                  <  SRR4302535.3850760/94‑1 (MQ=60)
                            TCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTAT                                                                  <  SRR4302535.852266/94‑1 (MQ=60)
                            TCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAT                                                                  >  SRR4302535.2717747/1‑94 (MQ=60)
                             CGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATC                                                                 >  SRR4302535.2935920/1‑94 (MQ=60)
                             CGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATC                                                                 >  SRR4302535.1809086/1‑94 (MQ=60)
                              GCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCT                                                                >  SRR4302535.2032721/1‑94 (MQ=60)
                              GCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCT                                                                <  SRR4302535.2971187/94‑1 (MQ=60)
                               CATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTG                                                               >  SRR4302535.3574309/1‑94 (MQ=60)
                               CATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTG                                                               >  SRR4302535.2817995/1‑94 (MQ=60)
                               CATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTG                                                               >  SRR4302535.3626567/1‑94 (MQ=60)
                               CATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTG                                                               <  SRR4302535.2460921/94‑1 (MQ=60)
                               CATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTG                                                               >  SRR4302535.2307980/1‑94 (MQ=60)
                                ATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGG                                                              <  SRR4302535.4370759/94‑1 (MQ=60)
                                ATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGG                                                              >  SRR4302535.2689610/1‑94 (MQ=60)
                                ATGATGCGCGATCGCCGGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGG                                                              >  SRR4302535.1897246/1‑94 (MQ=60)
                                   ATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGA                                                           <  SRR4302535.127937/94‑1 (MQ=60)
                                     GCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATC                                                         <  SRR4302535.3922563/94‑1 (MQ=60)
                                      CGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCG                                                        <  SRR4302535.4190009/94‑1 (MQ=60)
                                      CGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCG                                                        >  SRR4302535.3636304/1‑94 (MQ=60)
                                        CGATCGCCTGATCAATCACGCGATCCTCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAC                                                      <  SRR4302535.1976545/94‑1 (MQ=60)
                                         GATCGCCTGATCAATCAGGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACC                                                     <  SRR4302535.4254943/94‑1 (MQ=60)
                                         GATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACC                                                     >  SRR4302535.2955103/1‑94 (MQ=60)
                                          ATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCC                                                    <  SRR4302535.1633295/94‑1 (MQ=60)
                                            CGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCAC                                                  >  SRR4302535.1688099/1‑94 (MQ=60)
                                              CCTGATCAATCACGCGATCCGCTAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACAT                                                >  SRR4302535.4493007/1‑94 (MQ=60)
                                               CTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATC                                               >  SRR4302535.3447904/1‑94 (MQ=60)
                                               CTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATC                                               >  SRR4302535.1798624/1‑94 (MQ=60)
                                               CTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATC                                               >  SRR4302535.3977874/1‑94 (MQ=60)
                                               CTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATC                                               >  SRR4302535.1397822/1‑94 (MQ=60)
                                                TGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCA                                              >  SRR4302535.323896/1‑94 (MQ=60)
                                                 GATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAG                                             <  SRR4302535.3162901/94‑1 (MQ=60)
                                                 GATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAAACAGCATTTGTGTTGTATCTGGAGATCGACCCACATCAG                                             >  SRR4302535.207339/1‑94 (MQ=60)
                                                  ATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGG                                            >  SRR4302535.553522/1‑94 (MQ=60)
                                                   TCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGT                                           <  SRR4302535.426106/94‑1 (MQ=60)
                                                    CAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTG                                          <  SRR4302535.3971916/94‑1 (MQ=60)
                                                    CAACCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTG                                          <  SRR4302535.4014280/94‑1 (MQ=60)
                                                      ATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGA                                        >  SRR4302535.4575523/1‑94 (MQ=60)
                                                        CACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACG                                      >  SRR4302535.2185765/1‑94 (MQ=60)
                                                          CGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTC                                    <  SRR4302535.2425114/94‑1 (MQ=60)
                                                          CGCGATCCGCCAGGCCTGCGAAGACAAACTGGGAGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTC                                    <  SRR4302535.652143/94‑1 (MQ=60)
                                                           GCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCA                                   <  SRR4302535.11332/94‑1 (MQ=60)
                                                            GGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAGCCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAA                                  <  SRR4302535.2655345/94‑1 (MQ=60)
                                                            CGATCCGCCAGTCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAA                                  <  SRR4302535.2562888/94‑1 (MQ=60)
                                                            CGATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAA                                  >  SRR4302535.3742020/1‑94 (MQ=60)
                                                             GATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAAC                                 <  SRR4302535.2363153/94‑1 (MQ=60)
                                                             GATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAAC                                 >  SRR4302535.624940/1‑94 (MQ=60)
                                                             GATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAAC                                 <  SRR4302535.40167/94‑1 (MQ=60)
                                                              ATCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGTTCGACCCACATCAGGTGGACGGCAACG                                >  SRR4302535.4242212/1‑94 (MQ=60)
                                                               TCCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGT                               >  SRR4302535.3084758/1‑94 (MQ=60)
                                                                CCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTG                              <  SRR4302535.4172289/94‑1 (MQ=60)
                                                                CCGCCAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTG                              <  SRR4302535.4140460/94‑1 (MQ=60)
                                                                    CAGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACC                          >  SRR4302535.382330/1‑94 (MQ=60)
                                                                    CAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACC                          >  SRR4302535.1605927/1‑94 (MQ=60)
                                                                     AGGCCTGCGAAGACAATCTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCC                         <  SRR4302535.1334278/94‑1 (MQ=60)
                                                                     AGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCC                         >  SRR4302535.1831842/1‑94 (MQ=60)
                                                                     AGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCC                         >  SRR4302535.2636314/1‑94 (MQ=60)
                                                                     AGGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCC                         >  SRR4302535.1912435/1‑94 (MQ=60)
                                                                     AGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCC                         >  SRR4302535.3657990/1‑94 (MQ=60)
                                                                     AGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCAACC                         >  SRR4302535.3657991/1‑94 (MQ=60)
                                                                      GGCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCC                        <  SRR4302535.2026542/94‑1 (MQ=60)
                                                                       GCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCG                       <  SRR4302535.2330547/94‑1 (MQ=60)
                                                                       GCCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCG                       <  SRR4302535.1747782/94‑1 (MQ=60)
                                                                       GCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCG                       >  SRR4302535.3944548/1‑94 (MQ=60)
                                                                        CCTGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGC                      >  SRR4302535.2474504/1‑94 (MQ=60)
                                                                          TGCGAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCA                    >  SRR4302535.781681/1‑94 (MQ=60)
                                                                             GAAGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAAC                 >  SRR4302535.233117/1‑94 (MQ=60)
                                                                               AGACAAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACAC               <  SRR4302535.2496927/94‑1 (MQ=60)
                                                                                   AAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAG           <  SRR4302535.1399430/94‑1 (MQ=60)
                                                                                   AAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAG           <  SRR4302535.3361754/94‑1 (MQ=60)
                                                                                   AAACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAG           <  SRR4302535.2250189/94‑1 (MQ=60)
                                                                                   AAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAG           <  SRR4302535.3999790/94‑1 (MQ=60)
                                                                                    AACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGT          <  SRR4302535.288584/94‑1 (MQ=60)
                                                                                    AACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGT          <  SRR4302535.3255405/94‑1 (MQ=60)
                                                                                    AACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGT          <  SRR4302535.4364360/94‑1 (MQ=60)
                                                                                    AACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGG          >  SRR4302535.3472023/1‑94 (MQ=60)
                                                                                     ACTGGGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTG         <  SRR4302535.2790999/94‑1 (MQ=60)
                                                                                       TGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCG       <  SRR4302535.1379417/94‑1 (MQ=60)
                                                                                         GGGGGCTATCAGCAACCGACATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTT     <  SRR4302535.2309066/94‑1 (MQ=60)
                                                                                         GGGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTT     >  SRR4302535.2116076/1‑94 (MQ=60)
                                                                                         GGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTT     <  SRR4302535.1416800/94‑1 (MQ=60)
                                                                                          GGGGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTTT    <  SRR4302535.529107/94‑1 (MQ=60)
                                                                                            GGCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTTTCC  <  SRR4302535.4295514/94‑1 (MQ=60)
                                                                                            GGCGATCAGCAACCGGCATTTGTGTTGTAGCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTTTCC  <  SRR4302535.3105604/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGTCAACGTGCACCCCGCCAAACACGAAGTGCGTTTCC  >  NC_000913/4395741‑4395926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑