Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,431,142 T→C H154R (CAC→CGC)  ytfG ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,431,1420TC99.5% 669.0 / ‑4.8 216H154R (CAC→CGC) ytfGputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (108/107);  total (109/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

Only 100 of 239 total aligned reads displayed.

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAG  >  NC_000913/4431052‑4431230
                                                                                          |                                                                                        
  gCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTc                                                                                      <  2:1247711/93‑1 (MQ=255)
   cTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTcc                                                                                     <  1:2816920/93‑1 (MQ=255)
    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                    <  2:695942/93‑1 (MQ=255)
    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                    >  2:440191/1‑93 (MQ=255)
     aaTCCCGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                   <  2:660879/93‑1 (MQ=255)
      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                  >  2:3856674/1‑93 (MQ=255)
      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                  >  1:3438905/1‑93 (MQ=255)
       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                 >  2:230301/1‑93 (MQ=255)
       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                 <  1:1997762/93‑1 (MQ=255)
         aCGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgct                                                                               >  1:2222560/1‑93 (MQ=255)
             gTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCg                                                                           <  2:430900/93‑1 (MQ=255)
             gTGCCGCAGCTGCCGCATAATCTGCCCGCGGTGCTGACGCGATTTTGCCATCGGCCGCCGCACCGATAAATACGCAGCGTTCCAGTGCTGCCg                                                                           >  2:3774511/1‑93 (MQ=255)
                ccGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgggg                                                                        <  2:3361078/93‑1 (MQ=255)
                   caccTGCCGCATAATCGGCCCGCGTGGCTGAGGCGATTTTGCCATGGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                     <  2:2396276/90‑1 (MQ=255)
                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                     <  1:1398908/93‑1 (MQ=255)
                       tGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGc                                                                 >  1:100927/1‑93 (MQ=255)
                          cGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAg                                                              <  2:3715283/93‑1 (MQ=255)
                          cGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAg                                                              >  1:3143208/1‑93 (MQ=255)
                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAgg                                                             >  1:4200506/1‑93 (MQ=255)
                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAgg                                                             >  2:2979853/1‑93 (MQ=255)
                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                            >  1:2113225/1‑93 (MQ=255)
                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                            >  1:4056626/1‑93 (MQ=255)
                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGGGGTa                                                           >  2:128745/1‑93 (MQ=255)
                             aTAATCGGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                           <  1:2883714/93‑1 (MQ=255)
                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                          >  2:1049287/1‑93 (MQ=255)
                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                          >  1:1864562/1‑93 (MQ=255)
                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAg                                                          >  1:1444726/1‑93 (MQ=255)
                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCCGCCGGGGCGCTGGCGAGGTAg                                                          >  1:3532514/1‑93 (MQ=255)
                               aaTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGt                                                         >  1:2675819/1‑93 (MQ=255)
                               aaTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGt                                                         <  1:1213247/93‑1 (MQ=255)
                               aaTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGt                                                         >  2:1140576/1‑93 (MQ=255)
                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                        <  1:348106/93‑1 (MQ=255)
                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                        >  2:2499824/1‑93 (MQ=255)
                                 tCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGttt                                                       <  1:4415435/93‑1 (MQ=255)
                                  cTGCCCGCGTTGCTGAGGCGATTTTGCTATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtttt                                                      <  2:1604214/93‑1 (MQ=255)
                                  cTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtttt                                                      <  2:1312718/93‑1 (MQ=255)
                                   tGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTc                                                     <  1:2435091/93‑1 (MQ=255)
                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                <  1:1349229/93‑1 (MQ=255)
                                          gTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAc                                              >  2:3016676/1‑93 (MQ=255)
                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTTGCGAGGGAGTTTTCGCTGTAcc                                             >  2:2109777/1‑93 (MQ=255)
                                           tggCTGAGGCGATTTTGCCACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                             <  2:4158511/91‑1 (MQ=255)
                                             gCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAg                                           <  1:574341/93‑1 (MQ=255)
                                             gCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAg                                           >  1:3893038/1‑93 (MQ=255)
                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                        >  2:3305573/1‑93 (MQ=255)
                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                        >  2:2905222/1‑93 (MQ=255)
                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                        >  2:901787/1‑93 (MQ=255)
                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGGGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                        >  2:176216/1‑93 (MQ=255)
                                                 aGGCTATTTTGCCCTCGCCCCCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCGGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                       <  2:2792033/93‑1 (MQ=255)
                                                 aGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                       >  1:2962083/1‑93 (MQ=255)
                                                 aGGCGATTTGGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                       <  2:4075857/93‑1 (MQ=255)
                                                   gCGTTTTTCCCATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTg                                     <  2:2645504/93‑1 (MQ=255)
                                                    gaaTTTTGCCACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                    <  2:1724250/91‑1 (MQ=255)
                                                    caaTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                    <  2:3035993/91‑1 (MQ=255)
                                                    cGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                    <  2:3792514/93‑1 (MQ=255)
                                                    cGATTTTGCCATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgc                                    <  1:3953826/93‑1 (MQ=255)
                                                     gATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcg                                   >  1:3051909/1‑93 (MQ=255)
                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                  >  1:3758657/1‑93 (MQ=255)
                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                  >  1:2482774/1‑93 (MQ=255)
                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                  <  1:3819582/93‑1 (MQ=255)
                                                       ttttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgcg                                 >  1:778306/1‑92 (MQ=255)
                                                         ttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGAGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagc                               >  1:4264181/1‑93 (MQ=255)
                                                          tcccATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagca                              <  2:454933/91‑1 (MQ=255)
                                                          tGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagca                              <  1:3155764/93‑1 (MQ=255)
                                                           gcaaTCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGcagcag                             <  2:1413861/90‑1 (MQ=255)
                                                           gCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGcagcag                             <  1:861736/93‑1 (MQ=255)
                                                            ccAGCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAgg                            <  1:2053960/93‑1 (MQ=255)
                                                              aTCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgt                          <  2:3193185/93‑1 (MQ=255)
                                                                 gcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTa                       >  1:112101/1‑93 (MQ=255)
                                                                   ccgccgCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaaa                     <  2:3564994/93‑1 (MQ=255)
                                                                    cgccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAc                    >  2:3407225/1‑93 (MQ=255)
                                                                      ccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGa                  <  1:4182542/93‑1 (MQ=255)
                                                                      ccgcACCGATAAATACGCCGCGTTCCAGGGTTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGa                  <  2:2008900/93‑1 (MQ=255)
                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                 <  2:48132/93‑1 (MQ=255)
                                                                        gcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATg                >  1:3386688/1‑93 (MQ=255)
                                                                        gcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATg                >  1:1684309/1‑93 (MQ=255)
                                                                         cACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGc               <  2:4240058/93‑1 (MQ=255)
                                                                         cACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGc               <  1:2242209/93‑1 (MQ=255)
                                                                         cACCGATAAATACCCCCCGTTCCATTCCTGCCGGTGCGCTGGCGAGGTAGTTTTCGCTCTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGc               <  2:3599431/93‑1 (MQ=255)
                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGCTGcc              >  2:2286523/1‑93 (MQ=255)
                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc              >  1:3377568/1‑93 (MQ=255)
                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc              >  1:547740/1‑93 (MQ=255)
                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa             >  2:206530/1‑93 (MQ=255)
                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa             <  1:2628428/93‑1 (MQ=255)
                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa             >  1:3228924/1‑93 (MQ=255)
                                                                            cGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAg            >  2:74312/1‑93 (MQ=255)
                                                                            cGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAg            >  1:2857650/1‑93 (MQ=255)
                                                                             taTAAATACGCCGCGTTCCAGTGCTTCCGGGTCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGa           <  2:3623918/92‑1 (MQ=255)
                                                                             gATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCAAGa           >  1:2542769/1‑93 (MQ=255)
                                                                              aTAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGaa          >  2:3798447/1‑93 (MQ=255)
                                                                              aTAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGaa          >  2:786768/1‑93 (MQ=255)
                                                                              aTAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGaa          <  1:4579374/93‑1 (MQ=255)
                                                                               tAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAt         <  2:821196/93‑1 (MQ=255)
                                                                               tAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAt         >  2:893954/1‑93 (MQ=255)
                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGTCGAGGTAGTTTTCGCTGTGCCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc        <  1:1608106/93‑1 (MQ=255)
                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc        <  2:4624117/93‑1 (MQ=255)
                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc        <  2:3239717/93‑1 (MQ=255)
                                                                                 aaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATca       <  1:1172757/93‑1 (MQ=255)
                                                                                  aTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcag      <  2:112239/93‑1 (MQ=255)
                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATgagc     >  1:1067264/1‑93 (MQ=255)
                                                                                     cgccgcGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcca   >  1:4008590/1‑93 (MQ=255)
                                                                                          |                                                                                        
TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAG  >  NC_000913/4431052‑4431230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCA  >  NC_000913/4431049‑4431229
                                                                                             |                                                                                       
GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGC                                                                                         <  SRR4302535.1472403/94‑1 (MQ=60)
 CTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCG                                                                                        <  SRR4302535.2500273/94‑1 (MQ=60)
 CTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCG                                                                                        >  SRR4302535.4623950/1‑94 (MQ=60)
 CTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCG                                                                                        <  SRR4302535.2845425/94‑1 (MQ=60)
  TTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGT                                                                                       >  SRR4302535.2662318/1‑94 (MQ=60)
     GCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCC                                                                                    >  SRR4302535.4179132/1‑94 (MQ=60)
      CTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCG                                                                                   >  SRR4302535.988376/1‑94 (MQ=60)
      CTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCA                                                                                   <  SRR4302535.2836928/94‑1 (MQ=60)
       TAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAG                                                                                  <  SRR4302535.1577409/94‑1 (MQ=60)
        AATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGT                                                                                 >  SRR4302535.3462823/1‑94 (MQ=60)
         ATCTCGCGTGCCGCACCTCCCGCATAATCGGCCGCCGTTGCTGAGGCCATTTTGCCATCGCCCGCCGCACCGATAAATCCGCCGCGTTCCAGTG                                                                                <  SRR4302535.3525211/94‑1 (MQ=55)
         ATCCCGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTG                                                                                <  SRR4302535.1134508/94‑1 (MQ=60)
          TCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGC                                                                               <  SRR4302535.2012149/94‑1 (MQ=60)
           CACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCT                                                                              >  SRR4302535.2238380/1‑94 (MQ=60)
              GTGTGCAGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCTATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCC                                                                           <  SRR4302535.3405173/94‑1 (MQ=60)
              CCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCC                                                                           <  SRR4302535.500580/94‑1 (MQ=60)
                  GCCGCAGCTGCCGCATAACCTCCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGG                                                                       <  SRR4302535.1407592/94‑1 (MQ=60)
                   CCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCTATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGC                                                                      <  SRR4302535.2349059/94‑1 (MQ=60)
                   CCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGC                                                                      <  SRR4302535.2709331/94‑1 (MQ=60)
                      CAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCT                                                                   <  SRR4302535.1408895/94‑1 (MQ=60)
                         GTGCCTCATAATCTGCCCGCTTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGC                                                                <  SRR4302535.4009453/94‑1 (MQ=60)
                         CTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGC                                                                <  SRR4302535.3343781/94‑1 (MQ=60)
                         CTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGC                                                                >  SRR4302535.101638/1‑94 (MQ=60)
                         CTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGACGCACCGATAAATACGCAGCGTTCCAGTGCTGCCGGGGCGCTGGC                                                                >  SRR4302535.2611111/1‑94 (MQ=60)
                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                             >  SRR4302535.3165074/1‑94 (MQ=60)
                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                             <  SRR4302535.4503759/94‑1 (MQ=60)
                            CCGCATAATCTGCCCGCGTTGCTGAGGCGATTTGCCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAG                                                             <  SRR4302535.698759/94‑1 (MQ=60)
                             CGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGG                                                            >  SRR4302535.4229250/1‑94 (MQ=60)
                              GCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGT                                                           >  SRR4302535.4084398/1‑94 (MQ=60)
                              GCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGT                                                           >  SRR4302535.2128475/1‑94 (MQ=60)
                               CATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTA                                                          >  SRR4302535.1965788/1‑94 (MQ=60)
                               CATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTA                                                          >  SRR4302535.753139/1‑94 (MQ=60)
                               CATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTA                                                          >  SRR4302535.2520328/1‑94 (MQ=60)
                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                         >  SRR4302535.1878006/1‑94 (MQ=60)
                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                         >  SRR4302535.3440950/1‑94 (MQ=60)
                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAG                                                         >  SRR4302535.1455058/1‑94 (MQ=60)
                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGCAG                                                         >  SRR4302535.1182348/1‑94 (MQ=60)
                                ATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCCGCCGGGGCGCTGGCGAGGTAG                                                         >  SRR4302535.3557040/1‑94 (MQ=60)
                                ATAATCGGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAG                                                         <  SRR4302535.2904121/94‑1 (MQ=60)
                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGT                                                        >  SRR4302535.2694963/1‑94 (MQ=60)
                                 TAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGT                                                        <  SRR4302535.3973027/94‑1 (MQ=60)
                                  AATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTT                                                       <  SRR4302535.1221950/94‑1 (MQ=60)
                                  AATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTT                                                       >  SRR4302535.565548/1‑94 (MQ=60)
                                  AATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTT                                                       >  SRR4302535.447514/1‑94 (MQ=60)
                                   ATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTT                                                      <  SRR4302535.350504/94‑1 (MQ=60)
                                    TCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTT                                                     <  SRR4302535.4445654/94‑1 (MQ=60)
                                    TCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTT                                                     >  SRR4302535.2443173/1‑94 (MQ=60)
                                    TCTGCCCGCGTTGCTGAGGCGATTTTCCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTT                                                     <  SRR4302535.1068512/94‑1 (MQ=60)
                                      TGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCG                                                   <  SRR4302535.2452632/94‑1 (MQ=60)
                                       GCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGC                                                  >  SRR4302535.4075906/1‑94 (MQ=60)
                                       GCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGC                                                  <  SRR4302535.1876540/94‑1 (MQ=60)
                                          CGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGT                                               >  SRR4302535.2251317/1‑94 (MQ=60)
                                           GCGTTGCTGGGGCAATTTCGGCACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTA                                              <  SRR4302535.1358863/94‑1 (MQ=60)
                                           GCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTA                                              <  SRR4302535.1358864/94‑1 (MQ=60)
                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                             <  SRR4302535.860450/94‑1 (MQ=60)
                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                             >  SRR4302535.1155707/1‑94 (MQ=60)
                                            CGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAC                                             >  SRR4302535.2065646/1‑94 (MQ=60)
                                             GTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACC                                            >  SRR4302535.3542631/1‑94 (MQ=60)
                                              TTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCA                                           <  SRR4302535.1117120/94‑1 (MQ=60)
                                              TTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCA                                           <  SRR4302535.2121839/94‑1 (MQ=60)
                                               TGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAG                                          >  SRR4302535.3919725/1‑94 (MQ=60)
                                                GCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGC                                         <  SRR4302535.578370/94‑1 (MQ=60)
                                                 CTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCG                                        >  SRR4302535.1005311/1‑94 (MQ=60)
                                                 CTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCC                                        <  SRR4302535.2908575/94‑1 (MQ=60)
                                                  TGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCG                                       <  SRR4302535.3454932/94‑1 (MQ=60)
                                                   GAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGT                                      >  SRR4302535.2982874/1‑94 (MQ=60)
                                                     GGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTG                                    >  SRR4302535.3162644/1‑94 (MQ=60)
                                                      gcgtttgtccatTCGCCCGCCGCTCCGATAAATCCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGC                                   <  SRR4302535.1744957/82‑1 (MQ=60)
                                                       CGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCG                                  >  SRR4302535.3073285/1‑94 (MQ=60)
                                                       CGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCG                                  >  SRR4302535.2895384/1‑94 (MQ=60)
                                                       CGATTTTGCCATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCG                                  <  SRR4302535.3980817/94‑1 (MQ=60)
                                                        GATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGC                                 >  SRR4302535.3784643/1‑94 (MQ=60)
                                                        GATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGC                                 >  SRR4302535.2555119/1‑94 (MQ=60)
                                                        GATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGC                                 >  SRR4302535.2500676/1‑94 (MQ=60)
                                                         ATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCG                                >  SRR4302535.783774/1‑94 (MQ=60)
                                                         ATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCA                                <  SRR4302535.3845938/94‑1 (MQ=60)
                                                         ATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGGAGTTTTCGCTGTACCAGCCGTTGCGCA                                >  SRR4302535.2151099/1‑94 (MQ=60)
                                                           TTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGC                              >  SRR4302535.4390057/1‑94 (MQ=60)
                                                           TTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGAGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGC                              >  SRR4302535.4293384/1‑94 (MQ=60)
                                                           TTTGCCAGCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGC                              <  SRR4302535.3129718/94‑1 (MQ=60)
                                                             TGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAG                            <  SRR4302535.2332376/94‑1 (MQ=60)
                                                             TGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAG                            <  SRR4302535.3177681/94‑1 (MQ=60)
                                                             TGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAG                            >  SRR4302535.1582848/1‑94 (MQ=60)
                                                              GCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGG                           <  SRR4302535.867827/94‑1 (MQ=60)
                                                               CCAGCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGG                          <  SRR4302535.2068755/94‑1 (MQ=60)
                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGT                         <  SRR4302535.1413093/94‑1 (MQ=60)
                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGT                         >  SRR4302535.3291412/1‑94 (MQ=60)
                                                                CATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGT                         >  SRR4302535.3190058/1‑94 (MQ=60)
                                                                 ctccCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTG                        <  SRR4302535.2113826/90‑1 (MQ=60)
                                                                 ATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTG                        <  SRR4302535.1681494/94‑1 (MQ=60)
                                                                   CGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTA                      >  SRR4302535.112889/1‑94 (MQ=60)
                                                                    GCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAA                     >  SRR4302535.2930649/1‑94 (MQ=60)
                                                                     CCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAA                    >  SRR4302535.2454514/1‑94 (MQ=60)
                                                                     CCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAA                    <  SRR4302535.4275198/94‑1 (MQ=60)
                                                                      CCGCCGCACCGATAAATGCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                   <  SRR4302535.1911693/94‑1 (MQ=60)
                                                                      CCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                   <  SRR4302535.4228545/94‑1 (MQ=60)
                                                                      CCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAC                   <  SRR4302535.284983/94‑1 (MQ=60)
                                                                        GCCGCACCGATAAATACGCCGCGTGCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGA                 <  SRR4302535.4172023/94‑1 (MQ=60)
                                                                         CCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAT                <  SRR4302535.38500/94‑1 (MQ=60)
                                                                         CCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAT                <  SRR4302535.3465622/94‑1 (MQ=60)
                                                                         CCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAT                <  SRR4302535.4211160/94‑1 (MQ=60)
                                                                          CGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATG               <  SRR4302535.1395196/94‑1 (MQ=60)
                                                                          CGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATG               >  SRR4302535.3410253/1‑94 (MQ=60)
                                                                          CGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATG               >  SRR4302535.1696507/1‑94 (MQ=60)
                                                                           GCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGC              <  SRR4302535.3836388/94‑1 (MQ=60)
                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC             >  SRR4302535.551567/1‑94 (MQ=60)
                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC             >  SRR4302535.3401053/1‑94 (MQ=60)
                                                                            CACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCC             <  SRR4302535.2258161/94‑1 (MQ=60)
                                                                             ACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCA            >  SRR4302535.3251301/1‑94 (MQ=60)
                                                                              CCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAG           <  SRR4302535.2647262/94‑1 (MQ=60)
                                                                              CCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAG           >  SRR4302535.2877894/1‑94 (MQ=60)
                                                                               CGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGA          >  SRR4302535.3005052/1‑94 (MQ=60)
                                                                               CGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCAAGA          >  SRR4302535.2561080/1‑94 (MQ=60)
                                                                                GATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAA         >  SRR4302535.4072232/1‑94 (MQ=60)
                                                                                 ATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAT        <  SRR4302535.4610567/94‑1 (MQ=60)
                                                                                 ATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAT        <  SRR4302535.3750418/94‑1 (MQ=60)
                                                                                  TAAATACGCCGCGTGCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATC       <  SRR4302535.2723030/94‑1 (MQ=60)
                                                                                   AAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGTCGAGGTAGTTTTCGCTGTGCCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCA      <  SRR4302535.1619704/94‑1 (MQ=60)
                                                                                   AAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGAAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGCGTAAACGATGCCAGAATCA      >  SRR4302535.4289013/1‑94 (MQ=60)
                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG     <  SRR4302535.39197/94‑1 (MQ=60)
                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG     <  SRR4302535.3620560/94‑1 (MQ=60)
                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG     <  SRR4302535.1181130/94‑1 (MQ=60)
                                                                                    AATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAG     <  SRR4302535.3170392/94‑1 (MQ=60)
                                                                                     ATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATGAGC    >  SRR4302535.1074853/1‑94 (MQ=60)
                                                                                       ACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCA  >  SRR4302535.4036012/1‑94 (MQ=60)
                                                                                             |                                                                                       
GCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCA  >  NC_000913/4431049‑4431229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑