Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,440,519 A→G E187E (GAA→GAG)  ytfM → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,440,5190AG99.0% 599.1 / ‑3.2 193E187E (GAA→GAG) ytfMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (103/88);  total (104/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 197 total aligned reads displayed.

TTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAACGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGA  >  NC_000913/4440429‑4440608
                                                                                          |                                                                                         
ttATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCg                                                                                         >  1:1819566/1‑93 (MQ=255)
 tATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGt                                                                                        <  2:3578802/93‑1 (MQ=255)
   tttCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTa                                                                                      <  1:2567702/93‑1 (MQ=255)
   tttCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTa                                                                                      <  2:3547285/93‑1 (MQ=255)
   tttCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTa                                                                                      >  1:1582836/1‑93 (MQ=255)
   tttCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTa                                                                                      >  2:3275132/1‑93 (MQ=255)
    ttCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTAc                                                                                     <  1:982867/93‑1 (MQ=255)
     tCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTAcc                                                                                    <  2:1585935/93‑1 (MQ=255)
         tAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGCTACCGCtt                                                                                <  2:4581800/93‑1 (MQ=255)
          aGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCttt                                                                               >  1:4152635/1‑93 (MQ=255)
           gCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTg                                                                              <  2:739577/93‑1 (MQ=255)
            cGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTgg                                                                             <  1:226819/93‑1 (MQ=255)
             gAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTggg                                                                            >  1:158918/1‑93 (MQ=255)
             gAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTggg                                                                            >  1:1629355/1‑93 (MQ=255)
             gAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTggg                                                                            >  1:3841727/1‑93 (MQ=255)
               aTTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCa                                                                          >  1:2790073/1‑93 (MQ=255)
                tttACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAt                                                                         <  1:3747313/93‑1 (MQ=255)
                 ttACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtg                                                                        <  2:4180143/93‑1 (MQ=255)
                  tACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtgt                                                                       <  1:4071885/93‑1 (MQ=255)
                  tACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtgt                                                                       <  2:3432304/93‑1 (MQ=255)
                  tACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtgt                                                                       <  1:1112110/93‑1 (MQ=255)
                  tACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtgt                                                                       <  1:2852364/93‑1 (MQ=255)
                   aCCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAtgtg                                                                      >  1:835515/1‑93 (MQ=255)
                     cAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGAc                                                                    >  1:1973718/1‑93 (MQ=255)
                      aaaGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGAcc                                                                   >  2:1198482/1‑93 (MQ=255)
                      aaaGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGAcc                                                                   >  1:3006551/1‑93 (MQ=255)
                       aaGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCt                                                                  >  2:2700728/1‑93 (MQ=255)
                         gcgcAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCttt                                                                >  1:45557/1‑93 (MQ=255)
                         gcgcAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCttt                                                                >  1:2316231/1‑93 (MQ=255)
                         gcgcAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCttt                                                                <  1:3488911/93‑1 (MQ=255)
                           gcAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGa                                                              <  1:3080685/93‑1 (MQ=255)
                                 gggCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGGGTTACCGCTTTGGGCATGAGACCTTTGAAGGAta                                                        >  2:401420/1‑92 (MQ=255)
                                   gCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATcac                                                      >  1:1413198/1‑93 (MQ=255)
                                   gCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATcac                                                      >  2:1367265/1‑93 (MQ=255)
                                   gCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATcac                                                      <  2:2405649/93‑1 (MQ=255)
                                      ttGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACaaa                                                   <  2:3130051/93‑1 (MQ=255)
                                         cgcTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATcc                                                <  1:4522496/93‑1 (MQ=255)
                                          gcTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCcg                                               >  2:2132288/1‑93 (MQ=255)
                                            tCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCcgcg                                             >  2:547819/1‑93 (MQ=255)
                                              ggCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGAt                                           <  2:2764195/93‑1 (MQ=255)
                                               gCCTGTATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATg                                          <  1:573582/93‑1 (MQ=255)
                                                  tGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAAt                                       <  2:2934542/93‑1 (MQ=255)
                                                  tGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAAt                                       <  1:2926353/93‑1 (MQ=255)
                                                  tGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAAt                                       >  1:2685548/1‑93 (MQ=255)
                                                   gCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATa                                      >  2:4642948/1‑93 (MQ=255)
                                                   gCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATa                                      >  2:4508582/1‑93 (MQ=255)
                                                    cATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATAc                                     <  2:1665175/93‑1 (MQ=255)
                                                    cATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATAc                                     <  1:2922135/93‑1 (MQ=255)
                                                      tAAAGCCTTCTGGGATATTGATTATAACGGTGGCGAGTGTTACTGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATGCCt                                   <  2:82888/93‑1 (MQ=255)
                                                      tAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCt                                   >  2:2599536/1‑93 (MQ=255)
                                                      tAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCt                                   >  2:2025646/1‑93 (MQ=255)
                                                       aaaGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCtc                                  >  1:124142/1‑92 (MQ=255)
                                                         aGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCa                                >  2:3231317/1‑93 (MQ=255)
                                                         aGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCa                                >  1:4018587/1‑93 (MQ=255)
                                                         aGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATAAATACCTGCa                                <  2:1255137/93‑1 (MQ=255)
                                                          gCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCaa                               >  2:4125421/1‑93 (MQ=255)
                                                           ccTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCaaa                              >  1:2165005/1‑93 (MQ=255)
                                                            cTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCaaaa                             >  2:1088647/1‑93 (MQ=255)
                                                            cTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCaaaa                             >  1:4682306/1‑93 (MQ=255)
                                                             ttCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAAt                            >  2:4356563/1‑93 (MQ=255)
                                                             ttCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAAt                            >  1:3447259/1‑93 (MQ=255)
                                                             ttCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAAt                            <  1:3322013/93‑1 (MQ=255)
                                                              tCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATc                           >  1:4186411/1‑93 (MQ=255)
                                                              tCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATc                           <  1:2472123/93‑1 (MQ=255)
                                                                tGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTg                         <  2:4312640/93‑1 (MQ=255)
                                                                 gggATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTgg                        >  1:3710579/1‑93 (MQ=255)
                                                                  ggATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTggg                       >  2:1928075/1‑92 (MQ=255)
                                                                   gATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTg                      >  1:1337694/1‑93 (MQ=255)
                                                                    atatTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGc                     <  1:1942082/93‑1 (MQ=255)
                                                                    atatTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGc                     <  2:1717239/93‑1 (MQ=255)
                                                                     tatTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGAGcc                    >  1:2005578/1‑93 (MQ=255)
                                                                      atTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCg                   >  1:3382671/1‑93 (MQ=255)
                                                                      atTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCg                   >  2:3699426/1‑93 (MQ=255)
                                                                       ttGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGt                  >  1:3195981/1‑93 (MQ=255)
                                                                       ttGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGt                  >  1:695410/1‑93 (MQ=255)
                                                                         gATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGttt                >  1:78612/1‑93 (MQ=255)
                                                                         gATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGttt                >  2:1334313/1‑93 (MQ=255)
                                                                          aTTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTa               >  1:1121233/1‑93 (MQ=255)
                                                                          aTTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTa               >  1:2231690/1‑93 (MQ=255)
                                                                            tataACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTaaa             <  1:1912616/93‑1 (MQ=255)
                                                                            tataACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTaaa             >  2:2846638/1‑93 (MQ=255)
                                                                             ataACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAag            <  2:2260123/93‑1 (MQ=255)
                                                                             ataACAGTGGCGAGCGTTACCGCTTTCGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAag            <  2:3792391/93‑1 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           >  2:2116427/1‑93 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           <  1:1353616/93‑1 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           >  2:1408900/1‑93 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           >  2:558231/1‑93 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           >  1:3948083/1‑93 (MQ=255)
                                                                              taACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAaga           >  1:3508314/1‑93 (MQ=255)
                                                                               aaCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAagag          <  2:4294440/93‑1 (MQ=255)
                                                                               aaCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAagag          <  2:636100/93‑1 (MQ=255)
                                                                               aaCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAagag          <  1:2735476/93‑1 (MQ=255)
                                                                               aaCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAagag          <  2:272513/93‑1 (MQ=255)
                                                                                aCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAgg         >  2:4455628/1‑93 (MQ=255)
                                                                                aCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAgg         >  1:2133027/1‑93 (MQ=255)
                                                                                aCAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAgg         <  2:1639374/93‑1 (MQ=255)
                                                                                 cAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAggg        >  2:3738502/1‑93 (MQ=255)
                                                                                  aGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGc       <  2:179630/93‑1 (MQ=255)
                                                                                    tGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGa     <  1:4247690/93‑1 (MQ=255)
                                                                                       cGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGa  <  1:628226/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAACGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGA  >  NC_000913/4440429‑4440608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAACGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGTA  >  NC_000913/4440426‑4440611
                                                                                             |                                                                                            
AGGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAG                                                                                              >  SRR4302535.3728248/1‑94 (MQ=60)
AGGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAG                                                                                              <  SRR4302535.2891741/94‑1 (MQ=60)
AGGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAG                                                                                              >  SRR4302535.2144348/1‑94 (MQ=60)
 GGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGC                                                                                             >  SRR4302535.2593296/1‑94 (MQ=60)
  GTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCG                                                                                            >  SRR4302535.3426214/1‑94 (MQ=60)
  GTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCG                                                                                            >  SRR4302535.1832712/1‑94 (MQ=60)
     ATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTA                                                                                         >  SRR4302535.1594260/1‑94 (MQ=60)
      TTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTAC                                                                                        <  SRR4302535.2586173/94‑1 (MQ=60)
       TTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACC                                                                                       <  SRR4302535.989849/94‑1 (MQ=60)
       TTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACC                                                                                       <  SRR4302535.3391416/94‑1 (MQ=60)
         CGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGC                                                                                     >  SRR4302535.1281121/1‑94 (MQ=60)
         CGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGC                                                                                     >  SRR4302535.2265096/1‑94 (MQ=60)
          GATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCT                                                                                    <  SRR4302535.1940854/94‑1 (MQ=60)
           ATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTT                                                                                   >  SRR4302535.2804972/1‑94 (MQ=60)
            TAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTT                                                                                  >  SRR4302535.4181068/1‑94 (MQ=60)
            TAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTT                                                                                  >  SRR4302535.2208891/1‑94 (MQ=60)
             AGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTG                                                                                 >  SRR4302535.2700565/1‑94 (MQ=60)
             AGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTG                                                                                 >  SRR4302535.585819/1‑94 (MQ=60)
               CGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGG                                                                               >  SRR4302535.3868195/1‑94 (MQ=60)
               CGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGG                                                                               >  SRR4302535.160035/1‑94 (MQ=60)
               CGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGG                                                                               >  SRR4302535.1641121/1‑94 (MQ=60)
               CGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGG                                                                               <  SRR4302535.228381/94‑1 (MQ=60)
               CGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGG                                                                               >  SRR4302535.290159/1‑94 (MQ=60)
                 AATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCA                                                                             <  SRR4302535.3482253/94‑1 (MQ=60)
                 AATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCA                                                                             >  SRR4302535.2809930/1‑94 (MQ=60)
                 AATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCA                                                                             >  SRR4302535.4473633/1‑94 (MQ=60)
                 AATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCA                                                                             >  SRR4302535.3091840/1‑94 (MQ=60)
                  ATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCAT                                                                            >  SRR4302535.1985366/1‑94 (MQ=60)
                   TTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATG                                                                           <  SRR4302535.3773238/94‑1 (MQ=60)
                   TTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATG                                                                           <  SRR4302535.2815632/94‑1 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         >  SRR4302535.841374/1‑94 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         >  SRR4302535.4034591/1‑94 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         >  SRR4302535.1598580/1‑94 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         <  SRR4302535.4099751/94‑1 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         <  SRR4302535.2872573/94‑1 (MQ=60)
                     TACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTG                                                                         <  SRR4302535.1120046/94‑1 (MQ=60)
                      ACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGA                                                                        >  SRR4302535.3104818/1‑94 (MQ=60)
                       CCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGAC                                                                       >  SRR4302535.1987932/1‑94 (MQ=60)
                        CAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACC                                                                      >  SRR4302535.3027614/1‑94 (MQ=60)
                        CAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACC                                                                      >  SRR4302535.2582054/1‑94 (MQ=60)
                           AGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTT                                                                   >  SRR4302535.2332805/1‑94 (MQ=60)
                           AGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTT                                                                   >  SRR4302535.45903/1‑94 (MQ=60)
                            GCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTG                                                                  <  SRR4302535.3513131/94‑1 (MQ=60)
                              GCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAA                                                                <  SRR4302535.3102163/94‑1 (MQ=60)
                               CAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAG                                                               <  SRR4302535.238066/94‑1 (MQ=60)
                               CAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAG                                                               <  SRR4302535.4262778/94‑1 (MQ=60)
                               CAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAG                                                               <  SRR4302535.4572269/94‑1 (MQ=60)
                               CAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAG                                                               >  SRR4302535.1408001/1‑94 (MQ=60)
                                   TGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATC                                                           <  SRR4302535.1612609/94‑1 (MQ=60)
                                    GGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCA                                                          <  SRR4302535.1498919/94‑1 (MQ=60)
                                     GGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCAC                                                         >  SRR4302535.1423283/1‑94 (MQ=60)
                                     GGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCAC                                                         >  SRR4302535.1808187/1‑94 (MQ=60)
                                       CATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAA                                                       >  SRR4302535.692147/1‑94 (MQ=60)
                                            CGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCG                                                  <  SRR4302535.4553486/94‑1 (MQ=60)
                                               TCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAACGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGA                                               <  SRR4302535.3744497/94‑1 (MQ=60)
                                                  GCCTGTATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGA                                            <  SRR4302535.577607/94‑1 (MQ=60)
                                                    CTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAAT                                          >  SRR4302535.2704750/1‑94 (MQ=60)
                                                    CTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAAT                                          >  SRR4302535.4656076/1‑94 (MQ=60)
                                                     TGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATA                                         <  SRR4302535.2946982/94‑1 (MQ=60)
                                                       CATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACC                                       <  SRR4302535.2942746/94‑1 (MQ=60)
                                                        ATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCT                                      >  SRR4302535.2003744/1‑94 (MQ=60)
                                                         TAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTC                                     >  SRR4302535.125011/1‑94 (MQ=60)
                                                           AAGCCTTCTGGGATATTGATTATAACAGTGGCGGGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCA                                   >  SRR4302535.708062/1‑94 (MQ=60)
                                                           AAGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCA                                   >  SRR4302535.4046082/1‑94 (MQ=60)
                                                            AGCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAA                                  >  SRR4302535.3560339/1‑94 (MQ=60)
                                                             GCCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAA                                 >  SRR4302535.2180530/1‑94 (MQ=60)
                                                              CCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAA                                >  SRR4302535.3802815/1‑94 (MQ=60)
                                                              CCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAA                                >  SRR4302535.4713960/1‑94 (MQ=60)
                                                              CCTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCAGCGATGAATACCTGCTAAA                                >  SRR4302535.4197008/1‑94 (MQ=60)
                                                               CTTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAAT                               >  SRR4302535.3471229/1‑94 (MQ=60)
                                                                TTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATC                              >  SRR4302535.4215063/1‑94 (MQ=60)
                                                                TTCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATC                              <  SRR4302535.3345125/94‑1 (MQ=60)
                                                                 TCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCT                             <  SRR4302535.2965160/94‑1 (MQ=60)
                                                                 TCTGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCT                             <  SRR4302535.2489946/94‑1 (MQ=60)
                                                                   TGGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGG                           >  SRR4302535.3736281/1‑94 (MQ=60)
                                                                    GGGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGT                          >  SRR4302535.1358470/1‑94 (MQ=60)
                                                                     GGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTG                         >  SRR4302535.1347238/1‑94 (MQ=60)
                                                                     GGATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTG                         <  SRR4302535.2708851/94‑1 (MQ=60)
                                                                       ATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCC                       <  SRR4302535.1956071/94‑1 (MQ=60)
                                                                       ATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGAGCC                       >  SRR4302535.2020025/1‑94 (MQ=60)
                                                                       ATATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGAGTACTTTGAAGGATCACAAAACCGCGATGAATAACTGCAAAATCTGGTGCC                       >  SRR4302535.2416886/1‑94 (MQ=60)
                                                                        TATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCG                      >  SRR4302535.3406187/1‑94 (MQ=60)
                                                                         ATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGT                     >  SRR4302535.3218096/1‑94 (MQ=60)
                                                                         ATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGT                     >  SRR4302535.4070649/1‑94 (MQ=60)
                                                                         ATTGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGT                     >  SRR4302535.700288/1‑94 (MQ=60)
                                                                           TGATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTT                   >  SRR4302535.79177/1‑94 (MQ=60)
                                                                            GATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTA                  >  SRR4302535.1129228/1‑94 (MQ=60)
                                                                            GATTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTA                  >  SRR4302535.2247557/1‑94 (MQ=60)
                                                                              TTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAA                >  SRR4302535.3959180/1‑94 (MQ=60)
                                                                              TTATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAA                >  SRR4302535.1889165/1‑94 (MQ=60)
                                                                               TATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAG               <  SRR4302535.1926398/94‑1 (MQ=60)
                                                                                ATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGA              <  SRR4302535.827320/94‑1 (MQ=60)
                                                                                ATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGA              >  SRR4302535.3532695/1‑94 (MQ=60)
                                                                                ATAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGA              >  SRR4302535.3975036/1‑94 (MQ=60)
                                                                                 TAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATGCCTGCAAAATCTGGTGCCGTTTAAAGAG             <  SRR4302535.3284082/94‑1 (MQ=60)
                                                                                 TAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAG             <  SRR4302535.1363301/94‑1 (MQ=60)
                                                                                 TAACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAG             <  SRR4302535.2943454/94‑1 (MQ=60)
                                                                                  AACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGG            >  SRR4302535.2148401/1‑94 (MQ=60)
                                                                                  AACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGG            <  SRR4302535.2104967/94‑1 (MQ=60)
                                                                                  AACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGG            <  SRR4302535.2754994/94‑1 (MQ=60)
                                                                                  AACAGTGGCGAGCGTTACCGCTTGGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGG            <  SRR4302535.53341/94‑1 (MQ=60)
                                                                                   ACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGG           >  SRR4302535.4111546/1‑94 (MQ=60)
                                                                                   ACAGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGG           <  SRR4302535.1212583/94‑1 (MQ=60)
                                                                                     AGTGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCG         >  SRR4302535.228652/1‑94 (MQ=60)
                                                                                       TGGCGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGAT       <  SRR4302535.4276781/94‑1 (MQ=60)
                                                                                          CGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAG    <  SRR4302535.964794/94‑1 (MQ=60)
                                                                                          CGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAG    <  SRR4302535.632603/94‑1 (MQ=60)
                                                                                          CGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAG    >  SRR4302535.2503324/1‑94 (MQ=60)
                                                                                          CGAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAG    <  SRR4302535.2150190/94‑1 (MQ=60)
                                                                                           GAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGT   >  SRR4302535.1082644/1‑94 (MQ=60)
                                                                                           GAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGT   <  SRR4302535.1937566/94‑1 (MQ=60)
                                                                                           GAGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGT   >  SRR4302535.2182533/1‑94 (MQ=60)
                                                                                            AGCGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGTA  >  SRR4302535.1264461/1‑94 (MQ=60)
                                                                                             |                                                                                            
AGGTTATTTCGATAGCGAATTTACCAAAGCGCAGCTGGGCATTGCGCTCGGCCTGCATAAAGCCTTCTGGGATATTGATTATAACAGTGGCGAACGTTACCGCTTTGGGCATGTGACCTTTGAAGGATCACAAATCCGCGATGAATACCTGCAAAATCTGGTGCCGTTTAAAGAGGGCGATGAGTA  >  NC_000913/4440426‑4440611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑